VWR avantor 612-6857 User manual

Instruction Manual
VWR® BOTTLE TOP DISPENSER
With Recirculation valve
EU cat. no
Version 01 Issue 03/2022
NA cat. no 76319-582
76319-584
76319-586
76319-588
76319-590
76319-592
612-6857
612-6858
612-6859
612-6860
612-6861
612-6862

2. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve
Legal address of Manufacturer
Europe
VWR International bv,
Researchpark Haasrode 2020
Geldenaaksebaan 464 B-3001 Leuven
[phone]
be.vwr.com
United States
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
[phone]
www.vwr.com
Country of Origin
India
EN
Instruction Manual
01 - 22
DE
Bedienungsanleitung
45 - 66
FR
Mode d’emploi
23 - 44
ES
Manual de instrucciones
67 - 88
Table of Contents

Contents
Safety information
Package contents
Installation
Intended use
Symbols and conventions
Product specifications
Instructions for use
Troubleshooting
General maintenance
Accessories and spares
Technical service
Warranty
Appendix
3. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Every user must read and understand this operating manual before operation.
Follow general instructions for hazard prevention and safety instructions e.g. wear
protective clothing, eye protection and gloves.
Observe all specifications provided by reagent manufacturers.
When dispensing inflammable media, make sure to avoid the built up of static charge, e.g.
do not dispense into plastic vessels do not wipe instruments with a dry cloth.
Use the instrument only for dispensing liquids, with strict regard to the defined limitations of
use and operating limitations. Observe operating exclusions. If in doubt, contact the
manufacturer or supplier.
Always use the instrument in such a way that neither the user nor any other person is
endangered. When dispensing, the discharge tube must always point away from you or any
other person. Avoid splashes. Only dispense into suitable vessels.
Never press down the piston when the discharge tube closure is attached.
Never remove the discharge tube while the dispensing cylinder is filled.
Reagents can accumulate in the cap of the discharge tube. Thus, it should be cleaned
regularly.
Never carry the mounted instrument by the cylinder sleeve or the valve block.
Breakage or loosening of the cylinder may also lead to personal injury from chemicals.
Never use force on the instrument. Use smooth gentle movements to operate the piston
upwards and downwards. Use only original manufacturer’s accessories and spare parts.
Do not attempt to make any technical alterations. Do not dismantle the instrument any
further than is described in the Operation Manual.
Always check the instrument for visual damage before use.
If there is any sign of a potential malfunction (e.g. piston difficult to move, stuck valve or
leakage), immediately stop dispensing. Consult the 'Troubleshooting' section of this
Operation Manual and contact the manufacturer if needed.
Safety Information
VWR Bottle Top Dispenser may sometimes be used with hazardous materials, operations
and equipments. It is beyond the scope of this manual to address all of the potential risks
associated with its use in such applications. It is the responsibility of the user of this
instrument to consult and establish appropriate safety and health practice and determine
the applicability of regulatory limitations prior to use.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Package Contents
Description
Bottle Top Dispenser
Telescopic tube
Calibration Tool
Bottle Adapters
Dispenser Thread Reducer
Calibration Certificate
Operation Manual
Quantity
1
1
1
5
1
1
1
Installation
2. Fix the telescoping tube. The tube’s ends have different diameters.
Please select the correct side for your dispenser.
(Fig. 2)
Fig.1
Fig.2
3. Choose the correct adapter for the bottle.
The threaded platform base of dispenser has a 30 mm screw thread.
Five adapters are supplied to suit containers with a 28, 32, 38, 40, 45
mm and 30 mm (inbuilt adapter) screw neck.
(Fig. 3)
Fig.3
1. Adjust length of telescoping inlet tube to fit your particular reservoir.
If you require a longer tube, it is provided on request.
(Fig. 1)
5. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

4. Fix the adapter.
(Fig. 4)
5. Mount the dispenser-
Screw it to the reservoir by applying gentle hand torque applied to
the threaded platform base only. In case of removal, apply the same
technique to the base, in the opposite direction.
(Fig. 5)
6. Ready to Use
Intended Use
Do not operate the piston until the unit is safely and fully
mounted on the reservoir bottle.
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution.
Product Specifications
Specifications as per ISO 8655
Volume
Range (ml)
Increment
(ml)
Accuracy
±% ml
CV
±% ml
0.25-2.5 0.05 0.5 0.0125 0.2 0.005
0.5-5 0.1 0.5 0.025 0.2 0.010
1-10 0.2 0.5 0.050 0.1 0.010
2.5-30 0.5 0.5 0.150 0.1 0.030
5-60 10 0.5 0.300 0.1 0.060
10-100 20 0.5 0.500 0.1 0.100
Fig. 4
Fig. 5
VWR Bottle Top Dispenser is a general purpose laboratory instrument intended for use in
laboratories for dispensing reagents and chemicals which are compatible with the
instrument.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Symbols and Conventions
6. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Error Limits related to the nominal capacity (= maximum volume) indicated on the instrument,
are obtained when instrument and distilled water are equilibrated at ambient temperature
(20°C/68°F). Testing takes place according to DIN EN ISO 8655-6 with a completely
assembled instrument and with uniform and smooth dispensing.
Instructions for Use
Overview
The bottle top dispenser is designed for dispensing liquids directly from the reservoir
bottle. The instrument is calibrated according to the requirements of the DIN EN ISO
8655 – 5. When the instrument is correctly used, the dispensed liquid comes into contact
with only the following chemically resistant materials: PTFE, FEP and Borosilicate glass.
2. Volume Adjustment
Knob
3. Re-Circulation
Valve
1. Cap
7. Main Housing
4. Delivery
Pipe Cover
6. Nozzle Cap
8. Retainer Ring
9. Middle Body
10. Threaded
Platform Base
11. Bottle Adapter
5. Delivery Pipe
Nozzle
7. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Getting Started
Priming
Open the cap of the dispensing tube (Fig. 6). For safety hold the discharge
tube orifice on the inner wall of a suitable receiving vessel.
Fig. 6
Fig. 7
1. Set the valve to ‘Recirculate’. (Fig. 7)
For priming gently pull up the piston approx, 30mm and push it down
rapidly until the lower stop. Repeat this procedure 5 times
(Fig. 8).
2. Turn valve to ‘Dispense’ (Fig.9).
To avoid splashes when priming hold the discharge tube on the inner
wall of a suitable receiving vessel and dispense liquid to prime the
discharge tube until it is bubble free, Wipe away any remaining drops from
the discharge tube.
Fig. 8
Note :
Before using the instrument for the first time, ensure it is rinsed carefully and
discard the first few samples dispensed.
Avoid splashing.
Important Guidelines
Operating limitations
Fig. 9
This instrument is designed for dispensing liquids, observing the following physical limits:
● Use temperature from +15°C to +40°C (from 59°F to 104°F) of instrument and reagent
● Vapor pressure up to max. 600 mbar. Aspirate slowly above 300 mbar, in order to
prevent the liquid from boiling.
● Kinematic viscosity 500 mm2/s
(dynamic viscosity [mPas] = kinematic viscosity [mm2/s] x density [g/cm3)
● Density: up to 2.2 g/cm3
8. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

● Liquids, which form deposits may make the piston difficult to move or may cause
jamming (e.g.,) crystallizing solutions or concentrated alkaline solutions). If the piston
becomes difficult to move, the instrument should be cleaned immediately.
● Compatibility of the instrument for a specific application(e.g. trace material analysis, food
sector etc.) must be checked by the user.
● Approvals for specific applications, e.g. for production and administration of food,
pharmaceuticals and cosmetics are not available.
Operating Exclusions
Never use with-
● Liquids attacking FEP, PFA and PTFE (e.g. dissolved sodium azide*)
●Liquids attacking borosilicate glass (e.g. hydrofluoric acid)
● Hydrochloric acid > 40% | Tetrahydrofuran | Trifluoroacetic acid
●Explosive liquids (e.g. carbon disulfide)
●Suspensions (e.g. of charcoal) as solid particles may clog or damage the instrument
●Liquids attacking PP (cap)**
* Dissolved sodium azide permitted up to a concentration of max. 0.1%.
Storage Conditions
● Store the instrument and accessories only in clean conditions in a cool and dry place.
● Storage temperature: from – 20°C to +50°C (from – 4°F to 122°F)
● Always wear protective gloves when touching the instrument or the bottle, especially
when using dangerous liquids. When mounted to a reagent bottle, always carry the
instrument as shown in the figure (5).
● Never press down the piston when the cap is on. Avoid splashing the reagent.
The reagent can drip out from the discharge tube and cap.
(Fig. 6)
Operation
9. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Volume Setting
The Volume Adjustment Knob is simple and easy to
operate. There are two positions of the knob as shown in
Fig. 10-A
Position 1 : Locked Position | Position 2 : Unlocked Position
To set the Volume, follow these simple steps-:
1. Turn the Knob from Position 1 to Position 2 by rotating it
ANTICLOCKWISE as shown in Fig. 10-B1.
2. The slider is now loose and can be moved up and down.
3. Set your desired volume by aligning the pointer with the
scale.
4. To lock the set volume, turn the Knob from Position 2 to
Position 1 by rotating it CLOCKWISE as shown in
Fig. 10-B2 .
Wear protective clothing, eye protection and gloves. Liquid may accumulate in the cap.
To avoid splashes dispense slowly. Follow all safety instructions and observe limitations
of use and operating limitations.
1. Remove cap from the discharge tube. (Fig. 11)
2. When using the instrument (with recirculation valve) turn the
valve to Dispensing. (Fig. 12)
Fig.11
Fig.12
Dispensing
Pointer
Slider
Volume
Adjustment
Knob Fig.10
Fig.10 A
Fig.10 B1
Fig.10 B2
10. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

3. Hold the discharge tube orifice on the inner wall of a suitable
receiving vessel. (Fig. 13)
4. Gently lift the piston until the upper stop and then depress piston
slowly and steadily with minimal force until the lower stop.
(Fig. 14)
5. Wipe off the discharge tube against the inner wall of the receiving vessel.
6. Reattach cap to discharge tube.
(Fig. 15)
Fig.13
Fig.14
Fig.15
11. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Troubleshooting
Trouble Possible cause Solution
Stop dispensing immedately.
Loosen piston with circular
motion, but do not
disassemble.
Follow all cleaning instructions.
Formation of crystals, dirty
Air bubbles appear
in the Instrument
Piston difficult to move
Reagent with high vapor
pressure has been drawn
in too quickly
The instrument has not
been primed
Filling tube is loose or
damaged
Liquid reservoir is empty
Too fast filling action
Leaking Piston
Leaking discharge valve
Slowly draw in reagent.
Prime the instrument.
Push the filling tube on firmly. if
necessary cut off approx. 1 cm
of the tube at upper end and
then re-connect it or replace
filling tube.
Refill reservoir and prime unit.
Fill & Dispence slowly
Clean piston, If Problem persist
replace piston.
Clean by flushing throughly
with distilled water.
Dispensing not possible
Instrument not calibrated
Blocked Dispense nozzle Disassemble the dispense
nozzle and flush through
with distilled water.
Clean Unit by immersing valve
assembly in distilled water
Discharge valve stuck
Wrong Dispenser
Volume
Barrel does not
fill with liquid
Inlet tube not fitted
firmly
Connect inlet tube correctly.
Follow steps of user
calibration.
Filling Not Possible Volume adjustment to
minimum setting
Set to required volume.
12. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

General Maintenance
VWR Bottle Top Dispenser should be cleaned in the following situations:
●Immediately when the piston is difficult to move
●Before changing the reagent
●Prior to long term storage
●Prior to dismantling the instrument
●Prior to autoclaving
●Prior to changing the valve
●Regularly when using liquids which form deposits (e.g. crystallizing liquids)
● Regularly when liquids accumulate in the cap.
Pre Dis-assembly Procedure
All maintenance should be carried out wearing suitable eye protection and protective
clothing. If in doubt, consult your safety officer.
1. Make sure that the Dispenser is completely empty.
2. Place the instrument into an empty sink together with its reservoir.
3. Unscrew the threaded platform base from the reservoir and lift the dispenser’s intake
tube carefully out of the reservoir, whilst tapping it against the reservoir’s aperture to
shake off any droplets from the intake tube.
4. Hold the dispense nozzle over the aperture of the reservoir and apply gentle piston
strokes in order to return any contents into the reservoir.
5. Empty the instrument completely and flush thoroughly with distilled water.
6. If the piston barrel is still not completely clean, you need to dis-assemble the dispenser.
Refer Dis-assembling procedure given below.
Dis-assembly and assembly Procedure
1. Pull the cap outwards to expose the Calibration Nut.
(Fig. 16)
2. Unscrew the Calibration Nut with the help of calibration tool to
dis-assemble the Piston and shaft out of the main housing.
(Fig. 17)
Fig.16
Fig.17
13. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

3. After unscrewing pull out the shaft.
(Fig. 18)
4. Rinse the piston and shaft with deionized water.
(Fig. 19)
5. Clean the cylinder with a bottle-brush. If necessary carefully remove
deposits at the edge of the glass cylinder.
(Fig. 20)
6. Then flush all the parts of the instrument with deionized water.
(Fig. 21)
8. Snap back the cap to complete the assembly.
(Fig. 23)
Fig.18
Fig.19
Fig.20
Fig.21
Fig.22
Fig.23
7. Insert the piston completely into the cylinder and then reassemble the
instrument using the calibration tool by screwing back the piston.
(Fig. 22)
14. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Dis-assembling and Assembling the Delivery Pipe
1. Unscrew the chuck nut and pull out the delivery pipe.
(Fig. 24) & (Fig. 25)
2. Clean the delivery pipe with deionized water.
3. First push the delivery pipe into the lower housing till
it stops going in further. (Fig. 26)
4. Screw the chuck nut to complete the assembly.
(Fig. 27)
Fig.24
Fig.25
Fig.26
Fig.27
Autoclaving
This instrument is autoclavable at 121° C (250° F) 1 bar absolute (15 psi) with a holding time of
at least 15 minutes.
Note-Only the piston needs to be removed for autoclaving the instrument.
Dis-assembling for Autoclaving
1. Pull the cap outwards to expose the Calibration Nut.
(Fig. 28)
2. Unscrew the Calibration Nut with the help of calibration tool
to dis-assemble the Piston and shaft out of the main housing.
(Fig. 29)
Fig.28
Fig.29
15. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

3. After unscrewing pull out the shaft.
(Fig. 30)
4. This is the piston-shaft sub-assembly.
(Fig. 31)
5. Autoclave the two sub-assemblies at 121°C and 15 psi
pressure for 10-15 mins.
(Fig. 32)
Re-assembling after Autoclaving
1. Insert the piston completely into the cylinder and then reassemble the
instrument use in the calibration tool by screwing back the piston.
(Fig. 33)
2. Snap back the cap to complete the assembly.
(Fig. 34)
3. Dispenser is now ready for use.
(Fig. 35)
Re-calibation is required after autoclaving.
Fig.30
Fig.31
Fig.32
Fig.33
Fig.34
Fig.35
6. The volume adjustment knob should always be kept in the
‘unlocked’ position while autoclaving.
(Refer fig. 10.A)
16. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

User Calibration
VWR Bottle Top Dispenser has been laboratory calibrated at its nominal volume. However, due
to changes in environmental conditions and the viscosity of the media which you dispense, we
recommend gravimetric testing every 3-12 months.
Gravimetric volume testing according to DIN EN ISO 8655-6 is performed as follows:
Re-Calibration Procedure
1. Set the instrument to the nominal volume or any other volume which is
most commonly used by you. Follow the common rules for calibration
used in statistical quality control (ISO 8655-5).
Set the volume and dispense five full volumes of distilled water at 20°C
on an electronic balance to establish the actual mean volume of liquid
dispensed. If the gravitational average result varies from the volume
displayed, you should re-calibrate the instrument.
Fig. 38
2. For re-calibration pull the cap outwards to expose the Calibration nut.
(Fig. 37)
3. Using the calibration tool, turn the calibration nut clockwise to reduce
the volume and anticlockwise to increase the volume.
Repeat this procedure till the desired volume is achieved on the
electronic balance. (Fig. 38)
Fig. 37
Fig. 36
17. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Accessories and Spares
18. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve
Descripton Quantity NA Cat. No. EU Cat. No.
VWR ADAPTER ASSORTMENT DISENSER PK4 1 76319-594 612-6865
VWR ADAPTER BOTTLE TOP DSPN 38MM PK3 1 76319-602 612-6866
VWR ADAPTER BOTTLE TOP DSPN 45MM PK3 1 76319-598 612-6867
VWR DISCHARGE TUBE F/10-100ML NOZZLE 1 76319-580 612-6872
VWR ADAPTER BOTTLE TOP DSPN 32MM PK3 1 76319-596 612-6868
VWR ADAPTER BOTTLE TOP DSPN 40MM PK3 1 76319-600 612-6869
VWR DISCHARGE TUBE F/1-10ML NOZZLE 1 76319-578 612-6873
VWR ADAPTER BOTTLE TOP DSPN 28MM PK3 1 76319-604 612-6870
VWR ADAPTER BOTTLE TOP DSPN 25MM PK3 1 76319-606 612-6871

Technical service
Web Resources
Visit the VWR website at www.vwr.com for:
●Complete technical service contact information
●Access to the VWR Online Catalogue, and information about accessories and related products
●Additional product information and special offers
Contact us For information or technical assistance contact your local VWR representative
or visit.www.vwr.com
Warranty
VWR warrants that this product will be free from defects in material and workmanship for a
period of two (2) years from date of delivery. If a defect is present, VWR will, at its option and
cost, repair, replace, or refund the purchase price of this product to the customer, provided it
is returned during the warranty period. This warranty does not apply if the product has been
damaged by accident, abuse, misuse, or misapplication, or from ordinary wear and tear. If the
required maintenance and inspection services are not performed according to the manuals
and any local regulations, such warranty turns invalid, except to the extent, the defect of the
product is not due to such non-performance.
Items being returned must be insured by the customer against possible damage or loss. This
warranty shall be limited to the aforementioned remedies. IT IS EXPRESSLY AGREED THAT
THIS WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE
WARRANTY OF MERCHANTABILITY
Compliance with Local Laws and Regulations
The customer is responsible for applying for and obtaining the necessary regulatory
approvals or other authorisations necessary to run or use the Product in its local environment.
VWR will not be held liable for any related omission or for not obtaining the required approval
or authorisation, unless any refusal is due to a defect of the product.
19. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve

Appendix
Chemical Compatibility Chart
Chemicals from A to Z
The following tables enlist the most frequently
used reagents.
They provide useful information for the safe and
efficient use of dispenser.
All the safety precautions and recommendations
within this Operation Manual must be followed
carefully.
Code explanations:
A = Good resistance
B = Acceptable with limitations
C = Not recommended
1 = Acid vapours (better resistance with lower
concentration).
Do not leave the instrument on the bottle.
Rinse with distilled water after use.
2 = Risk of damage, softening or discoloration of
external parts through vapours.
Do not leave the instrument on the bottle.
Rinse with distilled water after use.
3 = Chemical degradation of glass parts
(plunger/barrel).
20. vwr.com I Instruction manual VWR®Bottle Top Dispenser with Recirculation Valve