VWR Zippette Pro User manual

Zippette Pro
Instruction Manual
Zippette Pro 0-30 ml manual priming
Zippette Pro 0-30 ml spring-loaded
North American Catalogue Number: 75856-470
European Catalogue Number: 613-5281
Version: 1
Issued: May 2017

ZIPPETTE PRO
2VWR |INSTRUCTION MANUAL |EN
United States Europe
Manufactured for: Address of legal manufacturer
VWR International, LLC VWR International bvba
100 Matsonford Rd Researchpark Haasrode 2020
Radnor, PA 19087 Geldenaaksebaan 464
800-932-5000 B-3001 Leuven
+ 32 16 385011
http://www.vwr.com http://be.vwr.com
Instruction Manual.............................. 1 - 15
Manuel d’instructions ....................... 16 - 30
Bedienungsanleitung ........................ 31 - 45
Manual de instrucciones ................... 46 - 60
Manuale d’istruzioni ......................... 61 - 75
Manual de instruções........................ 76 - 90
EN
FR
DE
ES
IT
PT

3EN |INSTRUCTION MANUAL |VWR
WARNING!
•Always observe general safety regulations (e.g. wear suitable eye,
face, hand and general protection).
•Follow this operating instruction manual.
•NEVER use force! Breakage of any part may lead to the operator
and/or other persons being exposed to hazardous substances.
•Do not use the instrument where ammable fumes may exist.
•NEVER use damaged or deformed components.
Contents
Warning .....................................................................3
Safety information......................................................4
Package contents ....................................................... 4
Installation..................................................................4
Restrictions of use ......................................................4
Before using the Zippette Pro ..................................... 4
Assembly.................................................................... 4
Inserting.....................................................................5
Specication............................................................... 7
Instructions for use..................................................... 7
Priming reservoir ........................................................ 7
The volume adjustment knob .....................................7
User calibration ..........................................................7
Troubleshooting ........................................................8
General maintenance..................................................9
Maintenance/Cleaning................................................ 9
Fluid-path for
cleaning or replacement ........................................... 10
Sterilisation/Autoclaving ........................................... 11
Accessories & Spares ............................................... 14
Adaptors .................................................................. 14
Reservoirs................................................................. 14
Spare parts ............................................................... 15
Technical service....................................................... 15
Warranty .................................................................. 15
Tap open
Tap closed

ZIPPETTE PRO
4VWR |INSTRUCTION MANUAL |EN
Safety information
•When using the instrument, take into consideration the chemical compatibility of the materials in
contact with the liquid
•Never leave the Zippette Pro on your work bench with the piston barrel full of liquid without securing
the dispense anti-drip tap into ‘Closed’ position
•Always check that the dispense anti-drip tap is in the ‘Open’ position before attempting to dispense
•Before adjusting volume always place receiving vessel under nozzle and open tap
•Never use force
•Use only original spare parts and original accessories (See pages 14-15).
•If in doubt, consult your safety ofcer
Package contents
The Zippette Pro kit comprises:
•Zippette Pro dispenser
•Adaptors:
38 mm, 40 mm and 45 mm
•PTFE inlet tube
•Return spring
Installation
Restrictions of Use
NEVER use the Zippette Pro with:
•Liquids which are not compatible with PTFE, PVDF, FEP, borosilicate
glass, alumina ceramic or those which might attack platinum-iridium
•Hydrouoric acid
•Liquids which contain solid particles
•Temperature limits of Zippette Pro and reagent are +15 °C to +40 °C
Before using the Zippette Pro
Check that the instrument has not been damaged in transit.
Assembly
The Zippette Pro is packed with the dispense nozzle attached and the inlet feed tube removed. The 300 mm
length of PTFE inlet tube provided should be trimmed to t your particular reservoir. The bottom end should
be cut diagonally at a length that is close to the bottom of the container when the Zippette Pro is assembled
to the container. Longer lengths of inlet tube are available on request.
The threaded platform base of the Zippette Pro has a 30 mm screw thread for use with the recommended
accessories displayed on page 14. The assembled Zippette Pro is screwed to the reservoir using gentle hand
torque applied to the Zippette Pro’s threaded platform base only. Removal should also be by means of hand
torque applied to this same base.
Do not operate the piston until the unit is safely and fully mounted on the reservoir bottle.

5EN |INSTRUCTION MANUAL |VWR
Inserting the return spring (when applicable)
To insert the return self-priming spring follow the steps below:
1. Make sure that the Zippette Pro is completely empty. For ease of operation, assemble the Zippette Pro onto
an empty reservoir bottle and turn the dispense anti-drip tap to ‘Open’ position.
2. Turn the volume adjustment knob, to set the Zippette Pro to the max dispense volume of 30 ml (Drawing 1).
3. Turn the xing ring which is located at the bottom of the Zippette Pro’s piston sleeve until its raised rib is
aligned with the front piston sleeve slot and remove by gently pulling down (Drawing 1). The xing ring
should stay on the barrel protection inner sleeve (Drawing 2).
4. Press the two xing levers on the back of the piston sleeve inwards (Drawing 2), and lever the back piston
sleeve away from the front sleeve assembly (Drawing 3).
5. Remove the front sleeve assembly from the piston locator and move the piston out of its glass barrel
(Drawing 3 & 4).
6. Carefully guide the piston through the spring, and locate the piston back into its glass barrel (Drawing 5).
7. Reassemble the front Zippette Pro piston sleeve (Drawing 5) making sure that the cursor locates under the
stop ring (Drawing 5).
8. Ret the back piston sleeve and pull up the xing ring (Drawing 6) – turn it half circle to locate it onto the
Zippette Pro piston sleeve (Drawing 7). The unit is now ready for use (see operating instructions page 7).
Drawing 1 Drawing 2
Cursor
Raised rib
Threaded
platform ring Threaded platform ring
Threaded platform base
Volume
adjustment
knob
Front sleeve
Back sleeve Back sleeve
Fixing levers
Fixing ring Barrel protection
sleeve

ZIPPETTE PRO
6VWR |INSTRUCTION MANUAL |EN
Drawing 3
Drawing 5
Drawing 4
Drawing 6 Drawing 7
Return spring
Stop ring
Cursor

7EN |INSTRUCTION MANUAL |VWR
Specification
Accuracy ≤±0.3% on maximum delivery and a precision ≤0.1%CV using distilled water at 20 ºC.
Testing takes place according to DIN EN ISO 8655 using maximum nominal volume of distilled water at
20°C, with a uniform and smooth dispense action.
Instructions for use
Priming reservoir
•Place a container under the Zippette Pro’s dispense nozzle.
•Turn the volume adjustment knob to 30 ml to enable free movement of the piston.
•Open the dispense anti-drip tap.
•Prime the unit with a few gentle up and down strokes, taking the piston right down to its lowest stop
position and lifting it up approximately 25 mm.
•Repeat until a steady, bubble free ow is visible in the dispense tube.
The volume adjustment knob
Turning the volume adjustment knob (Drawing 1) clockwise, will increase the volume to be dispensed.
Eachfull revolution of the knob represents 2 ml and is shown on the cursor aligned to the graduations.
For ne volume adjustments, turn the knob a quarter turn in either direction for 0.5 ml adjustment.
User calibration procedure
Your Zippette Pro has been laboratory calibrated to its nominal volume of 30 ml. However, due to changes
in environmental conditions and the viscosity of the media which you dispense, a recalibration might be
required. You can either recalibrate at regular intervals such as once a week or whenever you notice that the
dispensed volume is different from the volume displayed by the unit.
To fully recalibrate your Zippette Pro follow the procedure below:
1. Set the Zippette Pro to the nominal volume of 30 ml or any other volume which is the most common
volume you dispense.
2. Follow the common rules for calibration used in statistical quality control (ISO 8655-3:2002). Dispense
ve full volumes of distilled water at 20 °C to establish the actual mean volume of liquid dispensed. If the
average result varies from the volume displayed, you should recalibrate the Zippette Pro.
3. Using a Cross Head screw driver no.00, slightly undo the cursor’s screw and move the cursor up or down
until it aligns with the graduation of the actual volume established in step 2.

ZIPPETTE PRO
8VWR |INSTRUCTION MANUAL |EN
Troubleshooting
Problem Possible Remedy
Air bubbles appear in dispense tube Liquid reservoir is empty Refill reservoir and prime unit
Too fast filling action Pump liquid out, refill and dispense slower
Glass barrel is not sealing against
FEP ‘O’ ring
Unscrew the threaded platform ring and make
sure that the FEP ‘O’ ring is properly fitted into its
recess, if necessary stretch ‘O’ ring slightly using
fingertips
Leaking piston Clean piston. If problem persists, replace piston
assembly (Drawing 13)
Leaking discharge valve Clean unit by flushing through - if problem
persists, replace pedestal complete assembly
(Drawings 16 & 17)
Barrel does not fill with liquid Inlet tube not fitted correctly Connect inlet tube correctly
Inlet valve stuck Free inlet valve by inserting a thin rod into the
inlet aperture
Glass barrel is not sealing against
the FEP ‘O’ ring
Unscrew the threaded platform ring and make
sure that the FEP ‘O’ ring is properly fitted into its
recess, if necessary stretch ‘O’ ring slightly using
fingertips
Dispensing not possible Blocked dispense tube nozzle Disassemble the dispense tube and flush through
with cleaning fluid
Discharge valve stuck Clean unit by immersing platform in cleaning
fluid - if problem persists, replace pedestal
complete assembly (Drawings 16 & 17)
Wrong dispense volume Instrument not calibrated Calibrate the unit - See page 5 ‘User Calibration’
Leaking valves Clean platform base - if problem persists, replace
pedestal complete assembly (Drawings 16 & 17)
Liquid appears between glass barrel
and polypropylene protection sleeve
Glass barrel is not sealing against
the FEP ‘O’ ring
Unscrew the threaded platform ring and make
sure that the FEP ‘O’ ring is properly fitted into its
recess if necessary stretch ‘O’ ring slightly using
fingertips
Damaged FEP ‘O’ ring Replace FEP ‘O’ ring

9EN |INSTRUCTION MANUAL |VWR
General maintenance
Maintenance/Cleaning
Note: All maintenance should be carried out wearing suitable eye protection and protective clothing. If in
doubt, consult your safety ofcer.
1. Make sure that the Zippette Pro is completely empty and turn the anti-drip tap to ‘Open’ position.
2. Place the instrument into an empty sink together with its reservoir. Unscrew the threaded platform base
from the reservoir and lift the dispenser’s intake tube carefully out of the reservoir, whilst tapping it
against the reservoir’s aperture to shake off any droplets from the intake tube.
3. Hold the dispense nozzle over the aperture of the reservoir and apply gentle piston strokes in order to
return any syringe contents into the reservoir.
4. Flush out remaining glass barrel contents with distilled water or a suitable solvent. This will preserve the
smooth action of the piston and free action of the inlet and outlet valves. If the inlet valve does stick and
is not freed by ushing, it may be freed by gently inserting a thin rod into the inlet aperture and gently
pushing the ball off its seating. Re-check unit for operation.
5. Empty the instrument completely after cleaning, and ush through with distilled water.

ZIPPETTE PRO
10 VWR |INSTRUCTION MANUAL |EN
Fluid-path disassembly procedure for cleaning or replacement
Disassembly should only be undertaken AFTER the unit has been cleaned, using the standard cleaning
procedure described on page 9. For autoclaving follow steps 1-3.
1. Undo the piston sleeve xing ring by turning it to align with the sleeve slot and sliding it down from the
sleeve (Drawing 9 & 10).
2. Press the two xing levers on the back of the piston sleeve inwards, and lever the back piston sleeve away
from the front sleeve assembly (Drawing 10 & 11).
3. Remove the front piston sleeve (Drawing 12).
4. Pull out the piston assembly from the glass barrel (Drawing 13).
5. Undo the threaded platform ring of the base platform (Drawing 14) and turn the stop ring clockwise and
pull it off the assembly (Drawing 15).
6. Carefully slide off the xing ring and the threaded platform ring, together with the clear polypropylene
protection sleeve (Drawing 16).
7. Take out the glass barrel from the base platform. Under the glass barrel is an encapsulated FEP ‘O’ ring
which should be handled with extreme care (Drawing 16).
8. To remove the FEP dispense tube (Drawing 17).: First turn the anti-drip tap to the closed position then lift
off the dispense tube protective cover and pull out the dispense tube from its seating.
9. Reassemble the instrument following the above points in reverse order. Make sure that the FEP ‘O’ ring is
well located into the base platform’s recess.
Important points to observe:
•Do not use hard tools to scrape off residue of reagent from the piston
•Take special care not to damage the piston when inserting it into the glass barrel
•Bevelled inside edge of the glass barrel must be at the top end when tted
•Before tightening the threaded ring fully onto the threaded platform base, rotate the polypropylene
protection sleeve so that the tooth on its base locates properly into the notch on the threaded ring
•Make sure the front end of the dispense tube is clipped into the dispense tube protection cover from the
underside
•Do not use force in assembly or disassembly
•Always wear protective clothing and eye protection when disassembling or assembling your Zippette
Pro. Protective clothing and eye protection should also be worn when dispensing