Xylem YSI 2500 User manual

USER MANUAL
525021
YSI 2500 Biochemistry
Analyzer
OPERATIONS AND MAINT
ENANCE MANUAL

i
Table of Contents
1Introduction........................................................................................................................................1-1
Description..............................................................................................................................1-11.1
General Specifications............................................................................................................1-11.2
2Safety..................................................................................................................................................2-1
Important Safety Instructions..................................................................................................2-12.1
Explanation of Symbols..........................................................................................................2-22.2
3Getting Started...................................................................................................................................3-1
Unpacking...............................................................................................................................3-13.1
Warranty Card ........................................................................................................................3-23.2
What You Need ......................................................................................................................3-23.3
Major Components .................................................................................................................3-23.4
4Basic Setup ........................................................................................................................................4-1
Install Bottle Rack...................................................................................................................4-14.1
Connect Printer.......................................................................................................................4-24.2
Connect AC Power.................................................................................................................4-24.3
Align Sipper ............................................................................................................................4-44.4
Prepare and Install Buffer Solution.........................................................................................4-74.5 4.5.1 Prepare Buffer..................................................................................................................4-7
4.5.2 Install Buffer Solution.......................................................................................................4-7
Install Calibrator Solution........................................................................................................4-74.6
Prime the Fluid System ..........................................................................................................4-84.7
Install Enzyme Membranes ....................................................................................................4-84.8
Configure Instrument............................................................................................................4-104.9 4.9.1 Assign Chemistries to Probes........................................................................................4-10
4.9.2 Buffer..............................................................................................................................4-11
4.9.3 Calibrator........................................................................................................................4-11
Check Probe Currents..........................................................................................................4-124.10
5Running the Instrument....................................................................................................................5-1
Perform Daily Operational Checks.........................................................................................5-15.1 5.1.1 Enzyme Membrane Integrity Test....................................................................................5-1
5.1.2 Linearity Test....................................................................................................................5-3
5.1.3 Results .............................................................................................................................5-4
Sample Preparation................................................................................................................5-55.2
Run Batch...............................................................................................................................5-55.3 5.3.1 Create Batches ................................................................................................................5-5
5.3.2 Export...............................................................................................................................5-7
5.3.3 Load Samples ..................................................................................................................5-7
5.3.4 Run Samples....................................................................................................................5-8
5.3.5 Status...............................................................................................................................5-8
Run Stat..................................................................................................................................5-95.4
6Advanced Functions .......................................................................................................................6-12
Settings.................................................................................................................................6-126.1 6.1.1 System ...........................................................................................................................6-12
6.1.2 Display............................................................................................................................6-15
6.1.3 Date/Time.......................................................................................................................6-19

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Service..................................................................................................................................6-206.2 6.2.1 Sipper.............................................................................................................................6-20
6.2.2 Module............................................................................................................................6-23
Data ......................................................................................................................................6-276.3 6.3.1 Plate...............................................................................................................................6-27
6.3.2 Calibration......................................................................................................................6-30
Help ......................................................................................................................................6-306.4 6.4.1 About..............................................................................................................................6-30
6.4.2 Software.........................................................................................................................6-31
Connectivity..........................................................................................................................6-336.5 6.5.1 Ethernet Port..................................................................................................................6-33
6.5.2 RS232 Port.....................................................................................................................6-35
7Chemistry Setup................................................................................................................................7-1
Sample Volume ......................................................................................................................7-17.1
Measurement Parameter Information.....................................................................................7-17.2 7.2.1 D-Glucose (Dextrose) ......................................................................................................7-2
7.2.2 L-Lactate ..........................................................................................................................7-3
7.2.3 Simultaneous Glucose and L-Lactate..............................................................................7-4
8Operational Checks and Maintenance.............................................................................................8-1
Cleaning, Disinfecting, and Decontaminating.........................................................................8-18.1 8.1.1 Touch Panel.....................................................................................................................8-1
8.1.2 Decontamination Procedures...........................................................................................8-1
Daily Maintenance..................................................................................................................8-18.2 8.2.1 Empty the Waste Bottle....................................................................................................8-1
8.2.2 Check the Calibrator Bottle..............................................................................................8-1
8.2.3 Check the Buffer Bottle....................................................................................................8-2
8.2.4 Check for Leaks...............................................................................................................8-2
8.2.5 Clean up Spills .................................................................................................................8-2
8.2.6 Daily Operational Checks.................................................................................................8-2
Monthly Maintenance .............................................................................................................8-28.3 8.3.1Calibration Pumping System Maintenance......................................................................8-2
8.3.2 Buffer Pumping System Maintenance..............................................................................8-2
8.3.3 Bottle Cap Cleaning.........................................................................................................8-3
8.3.4 Sample Module Cleaning.................................................................................................8-3
Preventive Maintenance – 6 months or 1000 Hours..............................................................8-38.4 8.4.1 Sample Module Cleaning.................................................................................................8-3
8.4.2 Waste Module Cleaning...................................................................................................8-4
8.4.3 Enzyme Probe Cleaning ..................................................................................................8-4
8.4.4 Sipper pump Seal Replacement......................................................................................8-5
8.4.5 Bottle Tubing....................................................................................................................8-6
8.4.6 Pump Tubing Replacement .............................................................................................8-7
8.4.7 Install Waste Module........................................................................................................8-9
8.4.8 Waste Tubing...................................................................................................................8-9
8.4.9 Install Sample Module......................................................................................................8-9
8.4.10 Sipper Replacement..................................................................................................8-9
8.4.11 Calibrate Sipper.......................................................................................................8-11
8.4.12 Install Bottles ...........................................................................................................8-11
8.4.13 Install Membranes ...................................................................................................8-11
8.4.14 Prime Fluid System .................................................................................................8-11
Fuse Replacement................................................................................................................8-118.5 8.5.1 Fuse Requirements........................................................................................................8-11

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9Storage ...............................................................................................................................................9-1
Instrument Storage.................................................................................................................9-19.1
Enzyme Membrane Storage...................................................................................................9-19.2
Instrument Handling/Transport...............................................................................................9-19.3
10 Troubleshooting........................................................................................................................10-1
Printout Information ..............................................................................................................10-210.1
Troubleshooting Chart..........................................................................................................10-410.2
11 Principles of Operation.............................................................................................................11-1
Enzyme Sensor Technology.................................................................................................11-111.1
Measurement Methodology..................................................................................................11-211.2
Baseline Stability ..................................................................................................................11-211.3
Calibration.............................................................................................................................11-211.4
Linearity................................................................................................................................11-311.5
Temperature Compensation.................................................................................................11-311.6
Level Sensing.......................................................................................................................11-311.7
12 Warranty and Repair.................................................................................................................12-1
Limitation of Warranty...........................................................................................................12-112.112.1.1 Shipping Instructions ...............................................................................................12-1
12.1.2 Cleaning Instructions...............................................................................................12-2
YSI Factory Authorized Service Centers..............................................................................12-212.2
13 Notices .......................................................................................................................................13-1
Declaration of Conformity.....................................................................................................13-113.1
Radio and Television Interference Notice ............................................................................13-213.2
14 Appendix A – Software Flowchart...........................................................................................14-1
15 Appendix B – Concentration Unit Conversion.......................................................................15-1
Linearity Test. Concentration Unit Conversion.....................................................................15-115.1
FCN Membrane Integrity Test. Concentration Unit Conversion...........................................15-115.2
16 Appendix C – Effects of Selected Substances.......................................................................16-1
17 Appendix D – Line Power Cord and Plug Wiring...................................................................17-1
18 Appendix E - Reagents and Accessories ...............................................................................18-1

1-1
1 Introduction
Description1.1
The 2500 Biochemistry Analyzer is a laboratory instrument intended for use in research, food-processing and
bioprocessing applications. THE 2500 Analyzer IS NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN
PERFORMANCE EVALUATION.
The 2500 Analyzer can be set up to measure glucose and/or lactate in a sample.
User Features
Slim modular design
Easily expand analytes or chemistries
Multiple units use much less bench space
Proprietary enzyme electrode
Fast, accurate, and analyte-specific results
Uses biological separation technology
No hazardous chromatography solvents to dispose of
Icon-driven user interface with touchscreen
Easy to learn
Data download options
Save data on a USB drive, send it over the network,
or access it in a searchable database anytime
Onboard FAQs
Minimizes operator learning curve
General Specifications1.2
Sample size.................................................Adjustable from 10 to 50 microliters (aspirated volume), per module
Response time ............................................Enzyme Sensors
•Sample results in 60 seconds (average)
•Complete sample-to-sample cycle in less than 2 minutes
(May vary with analyte and sample matrix.)
Output signals:
Serial.......................................................... USB and RS232
Power requirement ...............................100–240 VAC ±10%
50–60 Hz ±5%
42 Watts nominal
Working environment:
Ambient temperature................................15–35°C
Relative humidity......................................10–75% (non-condensing)
Regulatory compliance.................................. ETL, CE, RoHS
61010-1 compliance:
•Pollution degree 2
•Installation category 2
•Altitude 2000m
•Atmosphere 75 KPa to 106 KPa
•Indoor use only
Instrument dimensions ................................12" wide x 20.5" deep x 15.75" high (30.5 cm x 52.1 cm x 40 cm)
Instrument weight........................................30.5 pounds (13.9 kilograms)

2-1
2 Safety
Important Safety Instructions2.1
DO NOT PLUG THE INSTRUMENT IN AT THIS TIME. You should apply power only when directed to do so in the setup
instructions.
1. Use ONLY the line power cord supplied with the instrument. When directed to, connect the plug to a matching three-
pronged wall receptacle.
2. Use ONLY fuses of the type supplied. Replacement power cords and fuses can be obtained from YSI, or your Dealer
Representative.
3. Do NOT use an extension cord without protective grounding.
4. Do NOT remove rear cover. There are no user serviceable parts inside.
5. Repairs are to be performed only by trained and approved personnel.
6. This instrument must be connected to a protectively grounded (earthed) outlet.
7. The following notice is provided in compliance with IEC 61010-1:2010.
See Appendix for mains plug wiring and fusing instructions.
8. If the equipment is used in a manner not specified by YSI, the protection provided by the equipment may be impaired.
WARNING: For RS232 or USB connection, equipment should be EN/CSA/UL 61010 or EN/CSA/UL 60950
approved only.
9. The mains (power) switch is for functional purposes ONLY. To disconnect the instrument from the mains supply,
unplug the mains power cord from the back of the instrument.
10. Personal protective equipment (PPE) recommended—protective gloves and safety goggles or glasses.

2-2
Explanation of Symbols2.2
WARNING
AVERTISSEMENT
Warning indicates that misuse of the instrument could result in
death or serious injury to a person.
Un avertissement indique qu'une mauvaise utilisation de l'instrument
peut entraîner la mort ou une blessure grave chez une personne.
CAUTION
ATTENTION
Caution, consult accompanying documents. Caution indicates
that misuse of the instrument could result in mild or serious injury
to a person and/or damage to equipment.
Attention, consulter la documentation jointe. Cette mise en garde
indique qu'une mauvaise utilisation de l'instrument peut entraîner une
blessure légère ou grave chez une personne et/ou un endommagement
du matériel.
Biological Risks
Risques biologiques
Chemical Irritant
Irritant chimique
Manufacturer
Fabricant
Authorized Representative in the European Union
Représentant agréé dans l'Union européenne
2776
Catalog number
Numéro de référence
18A100549
Lot number
Numéro de lot
YEAR-MO
Date of manufacture
Date de fabrication
YEAR-MO
Use by Date
Date limite d'utilisation
Temperature Limitation
Limite de température
REF

3-1
3 Getting Started
Unpacking3.1
When you unpack your new 2500 Analyzer for the first time, check the packing list to make sure you have received
everything listed. Note that reagents for the 2500 Analyzer are not packaged in the same carton as the instrument. If there
is anything missing or damaged, call the dealer from whom you purchased the 2500 Analyzer. If you do not know which of
our authorized dealers sold the system to you, call YSI Life Sciences Customer Service at 800 659-8895 or 937 767-
7241, and we'll be happy to help you.
1. After removing the instrument from the shipping box, tilt the display to the full upright position.
Figure 3-1
2. Grasp the hand hold in the right side cover of the instrument and pull up and out to remove the cover.
NOTE: Leave the cover off the instrument until you have aligned the sipper and installed the membranes as
described in the following sections.
3. Carefully cut the tie strap holding the sipper.
Figure 3-2
Remove tie strap

3-2
Warranty Card3.2
Please complete the Warranty Card or register your purchase online at www.ysi.com/customer-support/warranty-card .
This will record your purchase of this instrument in our computer system. Once your purchase is recorded, you will receive
prompt, efficient service in the event any part of your 2500 Analyzer should ever need repair.
What You Need3.3
Several things are needed in order to analyze samples using the 2500 Analyzer. The following list shows the basic items
required.
•2500 Instrument (with AC Power Cord)
•Bottle Rack with Reagent Level Sensing
•YSI 2357 Buffer
•YSI Calibrator Standard (YSI 2776 or 2747)
•YSI Linearity Standard(s)
•YSI Membrane(s) (YSI 2365 Glucose, 2329 Lactate)
•YSI 2901 Printer (optional)
Major Components3.4
Figure 3-3
Cover
Sipper
Display/
Touch Panel
USB Port
Station 2
Bottle Rack
Station 1
Waste Bottle
Buffer Bottle
Calibrator Bottle

3-3
Display Graphical color LCD covered by a touch screen
USB ports The USB ports allow a flash drive to be connected to the 2500
Analyzer to download sample results or upgrade the instrument’s
software. A USB port is located on the right side of the display. An
additional USB port is located on the rear of the instrument.
Sipper Can be raised, lowered, rotated, and moved horizontally to its
various positions.
The positions are: Calibrator Well, Sample Module, and Stations 1
and 2.
The Sipper senses fluid level to control immersion depth and detect
errors.
Station 1 Plate and rack holder accepts most standard plates/racks for batch
sampling of up to 96 samples.
Station 2 Test tube holder for manual sampling.
Buffer Pump Draws buffer from the buffer bottle, pumps it through the Sipper
Pump and the Sipper, and flushes the Sample Module.
Calibrator Pump Draws the standard solution from the Calibrator Bottle and fills the
Calibrator Well.
Figure 3-4
Sipper pump It retracts its piston to draw standard from the Calibrator Well or
sample from the sample stations.
It extends its piston to dispense standard or sample into the sample
module.
Sample Module
Sipper Pump

3-4
Sample module Made of clear acrylic plastic. Sensor probes are screwed into either
side of the module.
The immobilized enzyme membranes are mounted on O-rings which
act as fluid seals.
A reference or auxiliary electrode is housed in the temperature probe
and positioned at the back of the Sample module.
Stir Bar
(not shown) Plastic encapsulated magnet activated by a motor housed below the
sample module. Provides thorough mixing inside the sample module.
Buffer, Waste and
Calibrator Bottles Are conveniently located for maintenance.
Fluid levels are monitored by sensors.
Operation is automatically halted when the Buffer or Calibrator
Bottles are empty, or when the Waste bottle is full.
Rear Panel
Figure 3-5
Power Switch The main power switch is an on/off rocker switch (0-off and I-on)
located on the back of the instrument
Fuse Holder
The fuse holder houses the power line fuse and opens up for fuse
replacement.
Power Receptacle One end of the power cord (supplied) plugs into this receptacle,
while the other end plugs into a properly grounded electrical outlet.
The instrument will automatically adjust the voltage as needed.
Ethernet Port It allows connection to a network or router via an RJ45 Ethernet port.
RS232 Serial Port The RS232 connection is a standard DB9F connector. It is used to
interface with the YSI 2901 Printer.
Power
Receptacle
RS232 Port
Power Switch
Fuse
Holder
Ethernet Port
USB Port

4-1
4 Basic Setup
The following list describes the basic steps necessary for sampling with the 2500 Analyzer.
1. Install Bottle Rack
2. Connect Printer (optional)
3. Connect AC Power
4. Align Sipper
5. Prepare and Install Buffer solution
6. Install Calibrator Solution
7. Prime the Fluid System
8. Install Membrane(s)
9. Configure Instrument Chemistries
10. Check Probe Currents
Install Bottle Rack4.1
1. Install the Bottle Rack with Reagent Level Sensing onto the right side of the instrument by sliding the slots in the
tray over the pins on the side of the instrument.
2. Then remove the packing material holding the tubing to the right side of the instrument.
3. Next, connect bottle tubing and cables
a. Insert the large diameter waste tube into the hole in the waste bottle.
b. Connect one end of a short cable to the threaded fitting on the waste bottle cap and connect the other
end to the Waste (top) fitting on the instrument.
c. Connect the tubing marked “B1” and one end of a short cable to the fittings on the buffer bottle cap and
connect the other end of the cable to the Buffer (2nd) fitting on the instrument.
d. Connect the tubing marked “C1A” and one end of a long cable to the fittings on the calibrator bottle cap
and connect the other end of the cable to the CAL (bottom) fitting on the instrument.
Figure 4-1
Buffer Bottle
Waste
Buffer
CAL
Not Used
Calibrator Bottle
CAL
Waste Bottle

4-2
Connect Printer4.2
Connect the optional YSI 2901 Printer to the 2500 Biochemistry Analyzer using the data cable provided. The small RJ12
connector plugs into the bottom of the printer and the large DB9 connector plugs into the RS232 port on the back of the
analyzer. Refer to the instruction sheet included with the printer for details of printer operation.
Connect AC Power4.3
1. Plug the power cord (included with the 2500 Analyzer) into the power receptacle on the back of the instrument,
then into a properly grounded electrical outlet provided with a 15 or 20 Amp circuit breaker. The instrument will
automatically adjust the voltage as needed.
If you are located outside the United States, see Appendix D – Line Power Cord and Plug Wiring for Line Power
Cord and Plug Wiring.
WARNING: Keep your hands clear of the sipper while the instrument is in operation.
2. Turn the instrument on with the main power switch on the rear panel. After about 30 seconds, the Initializing
window should appear.
3. The first time the instrument is powered up, the software license window will appear. Touch [No] to prevent the
license screen from appearing each time the instrument is turned on.

4-3
4. Since the top cover of the instrument is removed, the message below will appear.
5. Touch [OK] to confirm, then touch the Settings icon.
6. Touch the Interlocks [On] button.

4-4
7. Touch [Yes] to confirm and disable the safety interlocks.
8. Touch [X] in the top left of the screen to return to the main display.
Align Sipper4.4
It is very important that the sipper be accurately adjusted.
WARNING: Keep your hands clear of the sipper while the instrument is in operation.
1. Touch the Service icon.
2. From the Sipper tab of the Service screen, touch [Home].
3. Once the sipper has moved to the home position, touch [Module 1].
4. The Select Location screen will appear. Select [Module 1]. The sipper will move to sample module 1 and should
be centered above the cone shaped opening in the top of the module.
If the sipper does not move, make sure the packing material was removed.

4-5
5. If the sipper is not centered, touch [Position] and use the arrow buttons to center the sipper.
6. Make certain the Sipper is centered, then touch [Save] at the bottom of the adjustment window.
Sipper Adjustment Position
Figure 4-2
7. To test the alignment of the sipper, Touch [Inject] to lower the sipper, then touch [Retract] to raise the sipper back
up. If necessary, touch [Position] and repeat the adjustment.

4-6
8. Once the sipper enters the sample module without hitting the cone, touch [Depth] to set the sipper depth. The
Select Location screen will appear.
9. Select [Module 1]. The tip of the sipper should be right at the top of the module. Use the arrow buttons to lower or
raise the sipper until the tip of the sipper is even with the top of the module.
10. Touch [Save] at the bottom of the adjustment window.
11. Check the sipper alignment at the Cal 1A and Drain 1 locations and adjust the position if necessary.

4-7
12. Once you have aligned the sipper properly and set the depth, sipper alignment for the Module is complete.
13. Check the sipper alignment for any rack or plate you intend to use at Station 1 (see 6.2.1 Sipper).
14. Touch [X] at the top left of the screen to return to the main display.
After you have aligned the sipper with the module, return to the Settings screen and touch the Interlocks button
and change it back to [On] to enable the safety interlocks.
Prepare and Install Buffer Solution4.5
Caution: To prevent possible damage due to an electrostatic discharge, do NOT touch the metal
tips of the connectors located at the ends of the bottle leads. Handle only the insulated section of
the connectors.
4.5.1 Prepare Buffer
1. Place about 500 mL of reagent water (distilled or deionized) into a 1000 mL flask or other clean container.
2. Add two packages of YSI 2357 powder buffer concentrate and stir.
3. Add more reagent water until the total volume of solution is between 900 and 1000 mL.
4. Stir as necessary until the buffer chemicals have completely dissolved.
4.5.2 Install Buffer Solution
5. Unscrew and remove the lid from the buffer bottle.
IMPORTANT: When adding fresh buffer to the Buffer Supply Bottle, make every effort to avoid contamination of the
lid and level sensor assembly.
6. Pour the prepared buffer into the buffer bottle.
7. Install the bottle in the rack.
8. Replace the bottle lid.
Install Calibrator Solution4.6
Caution: To prevent possible damage due to an electrostatic discharge, do NOT touch the metal
tips of the connectors located at the ends of the bottle leads. Handle only the insulated section of
the connectors.

4-8
1. Unscrew and remove the lid from the empty calibrator bottle.
IMPORTANT: make every effort to avoid contamination of the lid and level sensor assembly.
2. Mark the date of installation on the label of the new bottle of YSI calibrator solution (the working life is 30 days).
3. Place the new bottle of YSI 2747 or 2776 calibrator in the bottle rack. Verify that the Calibrator setting in the
Configure screen matches the model number of the bottle you install.
4. Screw the lid and level sensor assembly onto it.
Prime the Fluid System4.7
Please note that it may take from several minutes to more than an hour to initially stabilize the probes when setting up for
the first time (or after the power has been off for more than a few minutes).
To prime the fluid system:
1. From the Service screen, touch the [Module] tab.
2. Touch the button under B1 Pump to turn the buffer pump on.
3. The instrument will prime the buffer solution.
4. Once buffer flows from the end of the sipper, touch the button under B1 Pump to stop the pump.
5. Touch the button under Cal 1A Pump to turn it on.
6. The instrument will prime the calibrator solution.
7. Once calibrator flows from the calibrator well in the module, touch the button under Cal 1A Pump to stop the
pump.
Prime the calibrator bottle daily before calibrating or sampling to remove air bubbles from the tubing.
Install Enzyme Membranes4.8
Each biosensor probe installed in your instrument is fitted with a protective "shipping membrane" which must be removed
and replaced with a new membrane. Make sure you install the correct membrane for each chemistry you are
measuring.
Enzyme membranes are color-coded, red for glucose and gray for lactate. It is important that you install the specific
membrane as indicated on each probe (A or B).
.

4-9
Probe A is on the left when looking in from the side of the instrument
Figure 4-3
To install a membrane:
1. Make sure the top cover is removed from the instrument.
2. Next, unscrew the appropriate enzyme probe retainer and gently pull the probe out of the module.
3. Remove the existing O-ring membrane assembly from the end of the enzyme probe. A lint free tissue or toothpick
or pipet tip may be needed to unseat the old membrane. Be careful not to scratch the enzyme probe surface.
4. Examine the enzyme probe surface and remove any pieces of membrane that remained.
5. Open a cavity of the plastic membrane holder.
6. Rinse the membrane inside with a few drops of salt solution (YSI 2392).
7. Place one drop of salt solution on the enzyme probe face.
8. Using the plastic membrane holder, press the O-ring membrane assembly gently onto the probe face.
Figure 4-4
9. Wipe excess salt solution from the probe body.
10. Verify there is a stir bar in the module.
11. Then return the enzyme probe to the module.
12. Finger tighten the probe retainer so that the O-ring seals the probe in place. Do not overtighten.
13. Return the membrane holder to the foil pouch and refrigerate it.
14. Note the expiration date on the membrane package.
15. Repeat this procedure for the other enzyme probe.
You may want to maintain an instrument log book in which dates and lot numbers of reagents are recorded, along with
information from daily operational checks and other relevant information.
Probe B
Probe A