Zeiss Axioscope 5 User manual

Instruction Manual
ZEISS Axioscope 5, Axioscope 5/7 MAT
Upright Microscope for Routine and Entry-level Research

ZEISS Axioscope 5, Axioscope 5/7 MAT
Original Manual
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Suzhou Co., Ltd.
Modern Industrial Square 3-B, No.333 XingPu Road SIP
215126 Suzhou
China
Document Name: Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT
Order Number: 430035-7344-001
Revision: 13
Language: en-US
Effective from: 04/2022
© 2022 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced or
transmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any in-
formation or filing system. The right to make backup-copies for archiving purposes shall remain unaffected thereby. Any viola-
tions may be prosecuted as copyright infringements.
The use of general descriptive names, registered names, trademarks, etc. in this document does not imply that such names are
exempt from the relevant intellectual property laws and regulations and therefore free for general use. This shall also apply if
this is not specifically referred to. Software programs shall entirely remain the property of ZEISS. No program or subsequent up-
grade thereof may be disclosed to any third party, copied or reproduced in any other form without the prior written consent of
ZEISS, even if these copies or reproductions are destined for internal use at the customer's only, the only exception being one
single back-up copy for archiving purposes.

ZEISS Table of Contents
Table of Contents
1 About this Instruction Manual ........................................................ 8
1.1 Text Conventions and Link Types ........................................................................ 9
1.2 Explanation of Warning Messages and Additional Information............................ 10
1.3 Explanation of Symbols ....................................................................................... 11
1.4 Further Applicable Documents ............................................................................ 12
1.5 Contact............................................................................................................... 13
2 Safety ............................................................................................. 14
2.1 Intended Use ...................................................................................................... 14
2.1.1 Intended Purpose.................................................................................. 14
2.1.2 Lifetime................................................................................................. 15
2.1.3 Optical Risk Grouping............................................................................ 15
2.1.4 EMC Information................................................................................... 15
2.2 General Safety Information ................................................................................. 16
2.2.1 Requirements for Operators .................................................................. 16
2.2.2 Safe Operating Condition...................................................................... 16
2.3 Prevention of Hazards ......................................................................................... 16
2.3.1 Mechanical Hazards .............................................................................. 16
2.3.2 Electrical Hazards .................................................................................. 17
2.3.3 Hazards Generated with the Operating Environment............................. 17
2.3.4 Ergonomic Hazards ............................................................................... 17
2.3.5 Hazards Generated by Materials and Substances................................... 17
2.3.6 Hazards Generated by Radiation ........................................................... 18
2.3.7 Thermal Hazards ................................................................................... 18
2.4 Labels and Lights ................................................................................................ 19
2.4.1 Labels on the Axioscope........................................................................ 19
2.5 Safety Devices and Interlocks .............................................................................. 22
3 Product and Functional Description ................................................ 23
3.1 Main Components .............................................................................................. 24
3.1.1 Axioscope 5 TL...................................................................................... 24
3.1.2 Axioscope 5 TL/FL ................................................................................. 25
3.1.3 Axioscope 5 TL/RL Pol ........................................................................... 26
3.1.4 Axioscope 5 TL/RL................................................................................. 27
3.1.5 Axioscope 7 TL/RL MAT......................................................................... 28
3.1.6 Axioscope 5 Vario ................................................................................. 29
3.2 Controls and Functional Elements on Components.............................................. 30
3.2.1 Axioscope 5 TL Stand............................................................................ 30
3.2.2 Axioscope 5 TL/FL Stand ....................................................................... 32
3.2.3 Axioscope 5 TL/RL Pol Stand ................................................................. 34
3.2.4 Axioscope 5 TL/RL Stand ....................................................................... 36
3.2.5 Axioscope 7 TL/RL MAT Stand............................................................... 38
3.2.6 Axioscope 5 Vario Stand ....................................................................... 40
3.2.7 Functions of Stand Keys and Display Elements....................................... 41
3.2.8 Binocular Tubes..................................................................................... 43
3.2.9 Eyepieces .............................................................................................. 46
3.2.10 Nosepiece with Objectives .................................................................... 47
3.2.11 Condenser Carrier ................................................................................. 48
3.2.12 Condensers ........................................................................................... 48
3.2.13 Stages................................................................................................... 50
3.2.14 Reflector Inserts .................................................................................... 53
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 3

Table of Contents ZEISS
3.3 Light Manager Function ...................................................................................... 54
3.4 Microscopy and Contrast Methods...................................................................... 54
3.4.1 Transmitted Light Brightfield Microscopy Using the KÖHLER Method.... 54
3.4.2 Transmitted Light Darkfield Microscopy Using the KÖHLER Method...... 54
3.4.3 Transmitted Light Phase Contrast Microscopy ....................................... 55
3.4.4 Transmitted Light Differential Interference Contrast Microscopy............ 55
3.4.5 Transmitted Light PlasDIC Microscopy................................................... 55
3.4.6 Transmitted Light Polarization............................................................... 55
3.4.7 Reflected Light Brightfield Microscopy Using the KÖHLER Method........ 59
3.4.8 Reflected Light Darkfield Microscopy Using the KÖHLER Method .......... 59
3.4.9 Reflected Light DIC and C-DIC Microscopy ............................................ 59
3.4.10 Reflected Light TIC Microscopy ............................................................. 60
3.4.11 Reflected Light Polarization Microscopy ................................................ 62
3.4.12 Reflected Light Fluorescence Microscopy .............................................. 62
4 Installation...................................................................................... 63
4.1 Unpacking and Setting up the Microscope .......................................................... 63
4.2 Assembling the Upper Stand Part on the Stand Column ...................................... 64
4.3 Asssembling the Binocular Tube.......................................................................... 65
4.4 Assembling Components to the Binocular Tube .................................................. 66
4.5 Assembling Objectives ........................................................................................ 67
4.6 Assembling the Reflector Turret .......................................................................... 68
4.7 Assembling the Stage.......................................................................................... 69
4.7.1 Assembling the Stable Mechanical Stage and Specimen Holder............. 69
4.7.2 Assembling the Rotatable Mechanical Stage ......................................... 70
4.7.3 Assembling the Motorized Mechanical Stage on the Axioscope 7 Mo-
torized Material Stand........................................................................... 70
4.8 Assembling the Stage Carrier .............................................................................. 71
4.9 Assembling the Condenser Carrier....................................................................... 72
4.10 Assembling the Dry Darkfield Condenser............................................................. 73
4.11 Assembling the Transmitted Light Light Source ................................................... 74
4.11.1 Assembling the LED10 Light Source for Transmitted Light Illumination.. 74
4.11.2 Assembling the HAL 100 Light Source for Transmitted Light Illumina-
tion....................................................................................................... 75
4.12 Assembling the Reflected Light Light Source ....................................................... 77
4.12.1 Assembling the LED10 Light Source for Reflected Light Illumination...... 77
4.12.2 Assembling the HAL 100 Light Source for Reflected Light Illumination .. 77
4.12.3 Assembling the Adjustment Tool of HBO 100........................................ 77
4.12.4 Assembling the HBO 100 Light Source .................................................. 78
4.12.5 Assembling the Colibri 3 LED Light Source ............................................ 79
4.12.6 Assembling the HXP 120 V Light Source ............................................... 80
4.13 Connecting the Microscope to the Mains............................................................ 80
5 Operation........................................................................................ 81
5.1 Prerequisites for Commissioning and Operation .................................................. 81
5.2 Switching On the Microscope ............................................................................. 81
5.3 Adjusting ........................................................................................................... 82
5.3.1 Adjusting the Position of the Eyepieces ................................................. 82
5.3.2 Adjusting for Ametropia when Using Eyepiece Reticles.......................... 83
5.3.3 Adjusting the Upper Stand Part Vertically .............................................. 83
5.3.4 Adjusting the Height Stop on the Condenser Carrier ............................. 84
5.3.5 Adjusting the Height Stop on the Focusing Drive................................... 84
4 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001

ZEISS Table of Contents
5.3.6 Using the Light Manager Function ........................................................ 85
5.3.7 Setting the ECO/Permanent Mode......................................................... 87
5.4 Setting Up for Transmitted Light Techniques....................................................... 87
5.4.1 Setting Up for Transmitted Light Brightfield Microscopy Using the KÖH-
LER Method .......................................................................................... 87
5.4.2 Setting Up for Transmitted Light Darkfield Microscopy Using the KÖH-
LER Method .......................................................................................... 92
5.4.3 Setting the Darkfield Contrast with a Dry Darkfield Condenser .............. 94
5.4.4 Setting the Darkfield Contrast with an Immersion Oil Darkfield Con-
denser................................................................................................... 95
5.4.5 Setting Up for Transmitted Light Phase Contrast Microscopy................. 96
5.4.6 Setting Up for Transmitted Light DIC Microscopy .................................. 97
5.4.7 Setting Up for Transmitted Light PlasDIC Microscopy ............................ 98
5.4.8 Setting Up for Transmitted Light Polarization ........................................ 99
5.5 Setting Up for Reflected Light Techniques...........................................................105
5.5.1 Setting Up for Reflected Light Brightfield Microscopy Using the KÖHLER
Method................................................................................................. 105
5.5.2 Setting Up for Reflected Light Darkfield Microscopy Using the KÖHLER
Method................................................................................................. 108
5.5.3 Setting Up for Reflected Light DIC Microscopy ...................................... 109
5.5.4 Setting Up for Reflected Light C-DIC Microscopy................................... 110
5.5.5 Setting Up for Reflected Light TIC Microscopy....................................... 111
5.5.6 Setting Up for Reflected Light Polarization Microscopy – Proof of Bire-
flectance and Reflection........................................................................ 113
5.5.7 Setting Up for Reflected Light Fluorescence Microscopy........................ 114
5.6 Switching Off the Microscope .............................................................................116
6 Care and Maintenance ....................................................................117
6.1 Safety During Cleaning and Maintenance............................................................117
6.2 Maintenance Schedule........................................................................................118
6.3 Maintenance Work .............................................................................................118
6.3.1 Cleaning an Optical Surface .................................................................. 119
6.3.2 Removing Water-soluble Contamination ............................................... 119
6.3.3 Replacing the 12V, 50W Halogen lamp of the HAL50 Halogen Illumi-
nator..................................................................................................... 120
6.3.4 HAL100 Light Source ............................................................................ 121
6.3.5 Adjusting the HBO 100 Light Source ..................................................... 125
6.3.6 Replacing the LED Modules of the Colibri 3 LED Light Source................ 126
6.3.7 Recovering the Stage Travel Range in X-Axis ......................................... 127
6.3.8 Exchanging the Fuses in the Stand ........................................................ 128
6.3.9 Exchanging the Fuses in the External Power Supply for HAL 100........... 129
7 Troubleshooting .............................................................................130
7.1 Resetting the Microscope to the Factory Settings................................................134
8 Decommissioning and Disposal.......................................................135
8.1 Decommissioning................................................................................................135
8.2 Transport and Storage ........................................................................................135
8.3 Disposal ..............................................................................................................136
8.4 Decontamination ................................................................................................136
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 5

Table of Contents ZEISS
9 Technical Data and Conformity.......................................................137
9.1 Performance Data and Specifications ..................................................................137
9.2 Applicable Standards and Regulations.................................................................140
9.3 Usability of LED Modules for the Colibri 3 LED Light Source ................................140
10 Accessories and System Expansions................................................141
10.1 Binocular Tubes ..................................................................................................144
10.1.1 Binocular Tube 30°/23 .......................................................................... 144
10.1.2 Binocular Photo Tube Pol 20°/23 (100:0/0:100)..................................... 145
10.1.3 Binocular Ergo Photo Tube 20°/23 (100:0/0:100) .................................. 146
10.1.4 Binocular Ergo Photo Tube 15°/23 (50:50) ............................................ 147
10.2 Light Sources ......................................................................................................148
10.2.1 HAL 100 Light Source ........................................................................... 148
10.2.2 HBO 100 Light Source........................................................................... 150
10.3 Installing the Reversible Eyecups .........................................................................153
10.4 Analyzer Sliders...................................................................................................153
10.4.1 Analyzer Slider TL/RL, Fixed ................................................................... 153
10.4.2 Analyzer Slider TL/RL, with Lambda Plate, 360° Rotatable ..................... 154
10.4.3 Analyzer Slider TL/RL with Lambda Plate, each Rotatable +/- 10°........... 154
10.5 DIC Slider C 6x20 ................................................................................................154
10.6 Stop Sliders for Aperture and Luminous-Field Diaphragms...................................155
10.7 Stages.................................................................................................................156
10.7.1 Mechanical Stage, 75x50/240° Rotatable.............................................. 156
10.7.2 Rotary Stage Pol 360° with Clamping Device......................................... 158
10.7.3 Setting the Drive Length on the Stage Drive .......................................... 161
10.7.4 Removing the Additional Sleeves on the Ergonomic Stage Drive............ 161
10.7.5 Adjusting the Friction of the Coaxial Knurled Knobs on the Stage Drive. 162
10.7.6 Adjusting the Friction of the Coaxial Knurled Knobs on the Ergonomic
Stage Drive ........................................................................................... 163
10.8 Loading the Reflector Module.............................................................................164
10.8.1 Assembling Reflector Modules .............................................................. 164
10.8.2 Changing the Filters of a Reflector Module FL P&C................................ 165
10.8.3 Changing the Beam Splitter of a Reflector Module FL P&C .................... 166
10.9 Loading the Condenser .......................................................................................168
10.9.1 Assembling the Modulator Disk in the Condenser 0.9 BF Pol................. 168
10.9.2 Assembling the Slit-Diaphragm for PlasDIC into the Modulator Disk...... 169
10.9.3 Changing PhC DIC PlasDIC Diaphragms on the 0.9 BF DF PhC DIC
Achromatic-Aplanatic Condenser .......................................................... 170
10.10 Assembling the Sample Space Extension, 60 mm ................................................170
10.10.1 Removing the Cover of the Upper Stand Part ........................................ 171
10.10.2 Disconnecting the Cable Connections ................................................... 171
10.10.3 Removing the Upper Stand Part ............................................................ 172
10.10.4 Assembling the Sample Space Extension ............................................... 172
10.10.5 Assembling the Upper Stand Part on the Sample Space......................... 173
10.10.6 Establishing the Cable Connections ....................................................... 174
10.10.7 Assembling the Cover of the Upper Stand Part...................................... 174
10.11 Polarizers ............................................................................................................175
10.11.1 Polarizer D, Fixed, Removable ............................................................... 175
10.11.2 Polarizer D, 90°, Rotatable, Removable ................................................. 175
10.11.3 Polarizer, Fixed, with Lambda Plate, Rotatable ...................................... 176
10.11.4 Polarizer, Rotatable, with Color Filter Carrier ......................................... 176
10.11.5 Circular Polarizer D................................................................................ 177
10.11.6 Color Filter Carrier 3x for Filter d=32mm ............................................... 177
10.11.7 Low-power System for Objectives 2.5x/4x............................................. 178
6 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001

ZEISS Table of Contents
10.12 Assembling the Pol Components.........................................................................181
10.12.1 Assembling the Pol Object Guide .......................................................... 181
10.12.2 Assembling the Focusable Pol Eyepiece ................................................. 181
10.12.3 Centering the Objectives of the Polarization Stand ................................ 182
10.13 Axiocam 202 Mono/208 Color............................................................................183
10.13.1 Assembling the Axiocam 202 mono or Axiocam 208 color.................... 184
10.13.2 Operating Modes Using the Axiocam 202 mono/208 color .................. 185
10.14 Condenser, achromatic-aplanatic 0.9 BF DF PhC DIC...........................................191
10.14.1 Assembling the Condenser, Chromatic-aplanatic 0.9 BF DF PhC DIC...... 191
10.14.2 Centering the Darkfield Diaphragm of the Condenser ........................... 193
10.14.3 Centering the Annular Phase Diaphragm of the Condenser ................... 193
10.15 Assembling the Base Plate on the Stand..............................................................194
10.16 Assembling the Intermediate Plate for Analyzer Slider .........................................195
10.17 Assembling the Tube Lens Turret ........................................................................196
10.18 Assembling and Adjusting the Magnification Changer 4x....................................196
10.19 Replacing Filters in the Filter Wheel for Transmitted Light ...................................197
Revision History ..............................................................................198
Glossary ..........................................................................................199
Index...............................................................................................201
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 7

1 About this Instruction Manual |  ZEISS
1 About this Instruction Manual
This Instruction Manual (further called "document") is considered to be part of the Axioscope 5,
Axioscope 5/7 MAT, herein after referred to as "microscope".
This document contains basic steps and safety information that must be observed during opera-
tion and maintenance. Therefore, the document must be read by the operator prior to commis-
sioning and must always be available at the place of use of the microscope.
This document is an essential part of the microscope and, if the microscope is resold, the docu-
ment must remain with the microscope or be handed over to the new owner.
Axioscope 5 microscopes include:
§Axioscope 5 TL (430035-9201-000)
§Axioscope 5 TL HAL 50 (430035-9032-000)
§Axioscope 5 TL/FL (430035-9061-000)
Axioscope 5/7 MAT microscopes include:
§Axioscope 5 RL (430035-9091-000)
§Axioscope 5 TL/RL (430035-9121-000)
§Axioscope 5 TL/RL Pol (430035-9291-000)
§Axioscope 5 TL Pol (430035-9261-000)
§Axioscope 7 TL/RL MAT (430035-9330-000)
§Axioscope 5 Vario (430035-9150-000)
8 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001

ZEISS 1 About this Instruction Manual | 1.1 Text Conventions and Link Types
1.1 Text Conventions and Link Types
Example Explanation
The names of controls and important infor-
mation are shown in bold letters, for in-
stance:
Click Start. Software controls and GUI elements.
Press the Standby button. Hardware controls and elements.
Press Enter on the keyboard. Key on the keyboard.
Press Ctrl+Alt+Del. Press several keys on the keyboard simultane-
ously.
Select Tools>Goto Control Panel>Air-
lock.
Follow a path in the software.
Enter example.pdf in this field. Text to be entered by the user.
Programming and Macros Anything typed in literally during program-
ming, including, for example, macro codes,
keywords, data types, method names, vari-
ables, class names, and interface names.
Tab.1: Text conventions
Example Explanation
See: Text Conventions and Link Types [
} 9].Link to further information for this topic.
https://www.zeiss.com/corporate/int/
home.html
Link to a website on the internet.
Tab.2: Link types
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 9

1 About this Instruction Manual | 1.2 Explanation of Warning Messages and Additional Information ZEISS
1.2 Explanation of Warning Messages and Additional Information
DANGER, WARNING, CAUTION, and NOTICE are standard signal words used to determine the lev-
els of hazards and risks of personal injury and property damage. Not only the safety and warning
messages in the Safety chapter are to be considered also all safety and warning messages in
other chapters. Failure to comply with these instructions and warnings can result in both personal
injury and property damage and involve the loss of any claims for damages.
The following warning messages indicating dangerous situations and hazards are used in this doc-
ument.
DANGER
Type and source of danger
DANGER indicates an imminently hazardous situation which, if not avoided, will result in death
or serious injury.
WARNING
Type and source of danger
WARNING indicates a potentially hazardous situation which, if not avoided, may result in
death or serious injury.
CAUTION
Type and source of danger
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury.
NOTICE
Type and source of danger
NOTICE indicates a potentially harmful situation which, if not avoided, may result in property
damage.
Info
Provides additional information or explanations to help operator better understand the con-
tents of this document.
10 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001

ZEISS 1 About this Instruction Manual | 1.3 Explanation of Symbols
1.3 Explanation of Symbols
Symbol Explanation
CE marking (Conformité Européene)
CSA label: product tested by CSA to meet U.S. and Canadian standards.
CSA approval master number optionally given adjacent to this symbol
Manufacturer
Country of manufacture. "CC" is the country code, e.g. "DE" for Germany,
"CN" for China.
Date of manufacture optionally given adjacent to this symbol
Importer
Authorized representative in the European Community
In-vitro diagnostic medical device
Serial number
Catalogue number
WEEE label: Do not discard as unsorted waste. Send to separate collection
facilities for recovery and recycling
Tab.3: Explanation of symbols
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 11

1 About this Instruction Manual | 1.4 Further Applicable Documents ZEISS
1.4 Further Applicable Documents
Please take also note of the following documents:
Brochures and
Certificates
For brochures, ISO certificates, CSA certificates, and EU declarations of conformity ask your ZEISS
Sales & Service Partner.
Local and National
Health and Safety
Regulations
Observe local and national health and safety regulations for the location of installation and during
the use of the microscope.
Consult with your ZEISS Sales & Service Partner if these regulations are in conflict with the installa-
tion requirements of the microscope.
Safety Data Sheets Observe the enclosed safety data sheets. The instructions and guidelines given in the respective
safety data sheets must be complied with.
System and third-
party Components,
Accessories
Information about the individual components, enhancements, and accessories can be obtained
from your ZEISS Sales & Service Partner. Also refer to the documentation of third-party manufac-
turers.
Instruction
Manuals
For detailed information refer to the following Instruction Manuals of:
§Axiocam 208 color
§Axiocam 202 mono
§Light sources (e.g. HBO100, HXP120, HAL100, HAL50, Colibri3)
§Mechanical stage, 80X60, motorized
12 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001

ZEISS 1 About this Instruction Manual | 1.5 Contact
1.5 Contact
If you have any questions or problems, contact your local ZEISS Sales & Service Partner or one of
the following addresses:
Headquarters
Phone: [phone]
Fax: [phone]
Email: [email protected]
Microscopy Courses, Training, and Education
For information on microscopy courses, training, and education contact us on our homepage
(https://www.zeiss.com/microscopy/int/service-support/training-and-education.html#contact).
ZEISS Portal
The ZEISS Portal (https://portal.zeiss.com/) offers various services that simplify the daily work with
your ZEISS systems (machines and software). It is constantly improved and extended to meet your
needs and requirements better.
ZEISS Sales & Service Partner
You can find a ZEISS Sales & Service Partner in your area under https://www.zeiss.de/mikroskopie/
website/forms/sales-and-service-contacts.html.
Service Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 13

2 Safety | 2.1 Intended Use ZEISS
2 Safety
This chapter contains general requirements for safe working practices. Any person using the mi-
croscope or commissioned with installation or maintenance must read and observe these general
safety instructions. Knowledge of basic safety instructions and requirements is a precondition for
safe and fault-free operation. Operational safety of the supplied microscope is only ensured if it is
operated according to its intended use.
If any work is associated with residual risks, this is mentioned in the relevant parts of this docu-
ment in a specific note. When components must be handled with special caution, they are
marked with a warning label. These warnings must always be observed.
Any serious incident that has occurred in relation to the microscope and its components shall be
reported to these institutions:
§the competent authority of the Member State in which the user is established
§ZEISS
– for users within the EU:
Carl Zeiss Microscopy GmbH, Jena, Germany
– for users outside the EU:
Carl Zeiss Suzhou Co., Ltd., Suzhou, China
2.1 Intended Use
Improper use of the microscope and its components can easily lead to impairment of their func-
tion or even damage them. Damage caused by incorrect operation, negligence, or unauthorized
intervention, in particular by removing, modifying, or replacing parts of the microscope or its com-
ponents, cannot be held liable by the device manufacturer. Third-party devices or components
that are not expressly approved by ZEISS may not be used.
2.1.1 Intended Purpose
Axioscope 5 is an instrument for general microscopic imaging for the in vitro-examination of vari-
ous biological samples including samples collected from humans or animals. This imaging provides
information to further assess physiological and pathological conditions.
The microscope is intended to be used by trained professionals only.
Axioscope 5 microscopes include:
§Axioscope 5 TL (430035-9201-000)
§Axioscope 5 TL HAL 50 (430035-9032-000)
§Axioscope 5 TL/FL (430035-9061-000)
The Axioscope 5/7 MAT microscopes are universally usable microscopes for applications such as
materials analyses. It is not intended to either directly or indirectly generate medical diagnostic re-
sults.
Axioscope 5/7 MAT microscopes include:
§Axioscope 5 RL (430035-9091-000)
§Axioscope 5 TL/RL (430035-9121-000)
§Axioscope 5 TL/RL Pol (430035-9291-000)
§Axioscope 5 TL Pol (430035-9261-000)
§Axioscope 7 TL/RL MAT (430035-9330-000)
§Axioscope 5 Vario (430035-9150-000)
NOTICE
The catalogue number can be found on the type plate, see Labels and Lights [ } 19].
14 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001

ZEISS 2 Safety | 2.1 Intended Use
2.1.2 Lifetime
A microscope is an opto-electronic device. Its availability for use is significantly determined by the
performed maintenance. ZEISS guarantees the ability for maintenance and repair within eight
years after initial operation. This is ensured by a corresponding service and spare parts concept,
thus enabling the intended purpose within this duration.
2.1.3 Optical Risk Grouping
According to EN 62471 sources of optical radiation are classified into risk groups subject to their
potential photobiological hazard. Sources are classified into the following four groups according
to hazard, based on the emission limit as well as permissible exposure time before hazard ex-
ceeded.
Risk group Description
Exempt No photobiological hazard.
1 No hazard due to normal behaviourial limitations on exposure.
2 No hazard due to the aversion response to very bright light sources or
thermal discomfort.
3 Hazardous even for momentary exposure.
The following table lists the risk grouping of the available light sources/illumination units accord-
ing to the mentioned standard:
Light source/Illumination unit Risk group
Colibri3 3 (high risk)
HBO100 2 (moderate risk)
HXP120 2 (moderate risk)
LED modules 2 (moderate risk)
HAL100 2 (moderate risk)
HAL50 1 (low risk)
See also
2Usability of LED Modules for the Colibri 3 LED Light Source [}127]
2.1.4 EMC Information
The microscope is intended to be used in a basic electromagnetic environment. The electromag-
netic environment should be evaluated prior to operation of the microscope. Do not use the mi-
croscope in proximity to sources of strong electromagnetic radiation, as these can interfere with
proper operation.
Use of this microscope in a dry environment, especially if synthetic materials are present (synthetic
clothing, carpets, etc.), may cause electrostatic discharges that may cause erroneous results.
Electromagnetic interference (EMI) according to CISPR 11 Group 1:
§Class A (only Axioscope 5 Vario)
§Class B (all other Axioscope 5 and Axioscope 7 stands)
If in doubt, contact a ZEISS service representative.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 15

2 Safety | 2.2 General Safety Information ZEISS
2.2 General Safety Information
This document must be read before commissioning in order to ensure safe and uninterrupted op-
eration. Pay particular attention to all listed safety notes. Make sure, that
§the operating personnel has read and understood this manual, associated documents and
particularly all safety regulations and instructions, and applies them.
§the local and national safety and accident prevention regulations must be observed, as well as
the applicable laws and regulations in your country.
§this document is always available at the place of use of the microscope.
§the microscope is always in perfect condition.
§the microscope is secured against access by unauthorized persons.
§maintenance and repair work, retrofitting, removal or replacement of components, as well as
any other intervention in the microscope not described in this document, may only be carried
out by the manufacturer ZEISS or persons expressly authorized by ZEISS to do so.
2.2.1 Requirements for Operators
The microscope, components, and accessories may only be operated and maintained by autho-
rized and trained personnel. The microscope may only be used in accordance with this document.
If the microscope is not used as described, the safety of the user may be impaired and/or the mi-
croscope may be damaged.
Any unauthorized intervention or use other than within the scope of the intended use shall void
all rights to warranty claims. The regional regulations on health protection and accident preven-
tion must be observed at all times and during all work on and with the microscope.
Training Authorized ZEISS personnel will provide basic training in operating the microscope. As well as in-
formation on equipment safety and maintenance work that can be conducted by the operator.
The training will be documented by ZEISS and its completion is to be confirmed by the operator.
Special application training is offered for a fee. Current training dates, additional information and
the registration form can be found at https://www.zeiss.com/microscopy/int/service-support/train-
ing-and-education.html.
2.2.2 Safe Operating Condition
If circumstances occur which impair safety and cause changes in operating behavior, the micro-
scope must be shut down immediately and a ZEISS service representative should be informed.
The microscope may only be operated if the operating conditions are adhered to.
§Do not operate the microscope until you have completely read and understood the entire
documentation.
§Make sure that all protective cover panels are installed and all warning labels are available and
legible.
§Ensure conditions and take measures to prevent the build up of electrostatic charge on the
workplace.
2.3 Prevention of Hazards
This section summarizes potential hazards and recommended safety precautions. Failure to follow
the safety instructions and instructions may result in personal injury and property damage.
2.3.1 Mechanical Hazards
Crushing Hazards
due to Motorized
Components
The microscope contains motorized components. Fingers could be trapped. Do not reach into the
working area of motorized components when they are in operation.
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ZEISS 2 Safety | 2.3 Prevention of Hazards
Property Damage
due to Transport
There is a risk of injury and property damage if the microscope is improperly handled and trans-
ported.
§Only use the handle, if applicable, for transport of the microscope. Otherwise hold the micro-
scope with one hand and the base plate with the other hand.
2.3.2 Electrical Hazards
Voltage Hazards Risk of electric shock in case of contact with live parts.
The microscope must be plugged into a properly installed power socket with protective earth con-
tact using the supplied mains cable. The protective earth connection must not be impaired by the
use of extension cables.
Detachable mains supply cords must not be replaced with inadequately rated cords. Always use
the power cords supplied by ZEISS. When an unsuitable power cord is used, ZEISS can no longer
guarantee the electrical safety and functionality of the microscope.
§Shut down the microscope when not using the microscope.
§Disconnect the power supply before cleaning.
§Set up and operate the microscope so that the connectors are easily accessible.
§Position the microscope in a way so that you can easily unplug the power cable at any time.
2.3.3 Hazards Generated with the Operating Environment
Explosion Hazard Do not operate the devices included in the scope of supplies in a potentially explosive atmos-
phere, in the presence of volatile anesthetics or flammable solvents such as alcohol, gasoline or
similar substances.
Do not hold any flammable or easily combustible materials into the light beam.
Dirt, Dust, and
Moisture
Dirt, dust, and moisture can impair the microscope’s functionality.
§Shut down the microscope whenever it is not used and cover it with a dust protection cover.
§Always cover unused openings/ports with the corresponding system component or with blind
caps.
§Regular maintenance and cleaning according to the instructions in this document and accord-
ing to the instructions in the associated stand manual.
§Never expose the microscope to inadmissible climate conditions (high humidity and tempera-
ture).
2.3.4 Ergonomic Hazards
Prevention of
Musculoskeletal
Disorders
Musculoskeletal disorders (MSDs) affect the muscles, nerves, blood vessels, ligaments and ten-
dons. Workers in many different industries and occupations can be exposed to risk factors at
work, such as lifting heavy items, bending, reaching overhead, pushing and pulling heavy loads,
working in awkward body postures and performing the same or similar tasks repetitively. Employ-
ers are responsible for providing a safe and healthful workplace for their workers.
2.3.5 Hazards Generated by Materials and Substances
Infection Hazards Direct contact with the eyepieces can be a potential way of passing on bacterial and viral infec-
tions.
§The risk can be lowered by using personal eyepieces or eyecups. If eyepieces need to be disin-
fected frequently, ZEISS recommends to use the eyepieces without eyecups.
§To avoid infections, the use of personal protective equipment (PPE), e.g. gloves, for operation,
cleaning, and decontamination is highly recommended. Disposable gloves can be decontami-
nated with alcohol for example, if necessary, or should be changed frequently to minimize the
risk of contamination.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 17

2 Safety | 2.3 Prevention of Hazards ZEISS
Biological Hazard Biological substances/agents may pose a risk to the health of humans and other living organisms.
§Keep a logbook of the known biological substances/agents used when working with the mi-
croscope and show it to the ZEISS service representative before they perform any work on the
microscope.
Consumable
Hazards
Incorrect handling of consumables and cleaning agents can lead to property damage or skin and
eye injuries. Consumables that are not approved by ZEISS can lead to property damage. Consult
your ZEISS Sales & Service Partner to learn what consumables you can order and how to handle
them.
Hazard of Skin
Irritation
The immersion fluid can cause skin irritation.
§Avoid any contact with skin, eyes and clothes.
§Read and observe the safety data sheet of the immersion fluid.
§In the event of skin contact, wash the oil off with plenty of water and soap.
§In the event of eye contact, flush eyes with copious amounts of water for a minimum of 5
minutes. See a medical specialist if the irritation persists.
Hazardous
Substances
The microscope and other components can come into contact with various specimens and sub-
stances that can be hazardous to humans and the environment.
§Make sure that the microscope was not in contact with hazardous substances (check the lab-
oratory logbook); otherwise, the microscope must be cleaned/decontaminated/disinfected.
§Check the components also. If necessary, clean the components as meticulously as possible.
Label contaminated/infected components that cannot be properly cleaned.
§Contaminated parts shall not be returned to any ZEISS department. Decontaminated parts can
be sent to ZEISS accompanied by a signed „Customer Declaration of Decontamination“.
§Wear gloves.
2.3.6 Hazards Generated by Radiation
Optical Radiation
Hazards
Gas discharge lights, LED lights and other sources of white light emit strong optical radiation (e.g.
UV, VIS, IR). Optical radiation may cause damage to the skin and eyes. The extent of the damage
depends on the parameters such as wavelength, exposure time, mode of operation (continuous or
pulsed), etc.
§Avoid exposure of eyes and skin to radiation.
§Do not introduce reflective objects into the beam path.
§Never remove covers or cover panels during operation.
§Do not disable any interlock system elements.
§Use suitable protective equipment / protective clothing if required.
Electromagnetic
Radiation Hazards
In a domestic environment the microscope may cause radio interference, which may be mitigated
by relocating or re-orienting the equipment. The use of non-specified accessories, cables, or other
auxiliary parts from the field of information technology may lead to increased electromagnetic
emissions and reduced immunity to interference. Any integration into the system may result in a
degradation of the EMC performance.
2.3.7 Thermal Hazards
Burning Hazards Hot surfaces, radiation and/or aggressive chemicals can cause burns.
§Use suitable protective equipment / protective clothing if mandatory.
§Always observe the cooling time of the hot surfaces.
Heat Accumulation Covering the ventilation openings can lead to heat accumulation that may damage the micro-
scope and, in extreme cases, can cause a fire.
§Keep ventilation openings unobstructed at all times.
§Do not cover devices or openings emitting heat.
§Do not obstruct ventilation.
§Comply with minimum distance from walls.
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ZEISS 2 Safety | 2.4 Labels and Lights
2.4 Labels and Lights
This chapter shows labels and, where applicable, indicator lights.
All parts that may pose specific hazards are marked with warning labels.
Always observe all warning labels!
§Check all warning labels for availability and legibility.
§Immediately replace damaged or illegible warning labels.
In case a label is missing please contact your ZEISS service representative for free of charge re-
placement.
2.4.1 Labels on the Axioscope
1
2
5
3
4
6
Fig.1: Position of warning labels on microscopes with LED light source for transmitted light
7
Fig.2: Position of warning labels on microscopes with HAL100 for transmitted light and
HBO100 for reflected light
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 19

2 Safety | 2.4 Labels and Lights ZEISS
5
Fig.3: Position of the warning label on microscopes with LED light source for reflected light
8
Fig.4: Position of the warning label on the Axioscope7 stand using the motorized 80x60 me-
chanical stage
6
Fig.5: Position of the warning label on the power supply for two HAL100 lamps
6
9
Fig.6: Position of the warning labels on the power supply unit for HBO100
20 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001