Zeiss Axiocam 105 color User manual

Instruction Manual
ZEISS Axiocam 105 color
Version R2

ZEISS Axiocam 105 color
Original Manual
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
Document Name: Instruction Manual ZEISS Axiocam 105 color
Order Number: 426555-7010-001
Revision: 2
Language: en-US
Effective from: 09/2022
© 2022 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor
reproduced or transmitted in any form or by any means - including electronic or mechanic methods, by photocopy-
ing, recording or by any information or filing system. The right to make backup-copies for archiving purposes shall
remain unaffected thereby. Any violations may be prosecuted as copyright infringements.
The use of general descriptive names, registered names, trademarks, etc. in this document does not imply that
such names are exempt from the relevant intellectual property laws and regulations and therefore free for general
use. This shall also apply if this is not specifically referred to. Software programs shall entirely remain the property
of ZEISS. No program or subsequent upgrade thereof may be disclosed to any third party, copied or reproduced in
any other form without the prior written consent of ZEISS, even if these copies or reproductions are destined for
internal use at the customer's only, the only exception being one single back-up copy for archiving purposes.

Content ZEISS
Content
1 About this Instruction Manual....................................................... 5
1.1 Introduction ............................................................................................... 5
1.2 Text Conventions and Link Types................................................................ 5
1.3 Explanation of Warning Messages and Additional Information ................... 6
2 Safety ............................................................................................ 8
2.1 Intended Purpose ....................................................................................... 8
2.2 General Safety Information......................................................................... 8
2.2.1 Requirements for Operators........................................................ 8
2.2.2 Safe Operating Condition ........................................................... 8
2.3 Prevention of Hazards ................................................................................ 9
3 Technical Data and Conformity ..................................................... 10
3.1 Axiocam 105 color R2 ................................................................................ 10
3.1.1 Spectral Sensitivity...................................................................... 11
3.2 Applicable Standards and Regulations ........................................................ 11
4 Shipment ....................................................................................... 12
5 Connecting the Camera ................................................................. 13
5.1 Installing the interface card ........................................................................ 13
5.2 Mounting the camera on the microsope..................................................... 13
5.3 Connecting the camera with PC ................................................................. 13
5.4 Function indicator ...................................................................................... 14
6 Installing Software and Drivers ..................................................... 15
7 Care and Maintenance................................................................... 16
7.1 Optical System ........................................................................................... 16
7.2 Cleaning the infrared filter or protective glass............................................. 16
8 Troubleshooting ............................................................................ 17
8.1 The camera does not appear in the menu of selectable cameras ................ 17
8.2 You don't see a camera image on your screen ........................................... 17
8.3 The color of my image does not correspond to the impression through the
ocular......................................................................................................... 17
8.4 The performance of the camera is not consistent or is too slow ................. 17
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 3

Content ZEISS
9 Disposal......................................................................................... 18
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 4

1 About this Instruction Manual | 1.1 Introduction ZEISS
1 About this Instruction Manual
This Instruction Manual (further called "document") is considered to be part of the microscope
camera, herein after referred to as the "product" or the "camera".
This document contains basic steps and safety information that must be observed during opera-
tion and maintenance. Therefore, the document must be read by the operator prior to commis-
sioning and must always be available at the place of use of the product.
This document is an essential part of the product and, if the product is resold, the document must
remain with the product or be handed over to the new owner.
1.1 Introduction
This camera is a high-resolution digital microscope camera with a color CMOS sensor and USB 3.0
interface for light microscopy applications. To make it easier for you to set up the camera, follow
the instructions in these chapters step by step. In this manual the interface between the camera
and the computer is called USB 3.0.
Content This document contains all the information you need to set up the microscope, start the software,
and perform troubleshooting and maintenance. It contains the following chapters:
Chapter Content
About this Instruction Manual Introduction and overview about this manual.
Safety Important information on safe handling with the camera.
Read this chapter before unpacking the camera and
putting it into operation.
Technical Data and Conformity Here you will find the camera's technical data.
Shipment The contents of delivery and optional attachments will be
described here.
Connecting the Camera In this chapter you will find detailed instructions on con-
necting and using the camera.
Installing Software and Drivers Here you will learn how to install ZEISS software and cam-
era drivers.
Care and Maintenance This chapter describes some measures for the maintenance
and care of your camera. In case of greater damage, always
contact ZEISS support.
Troubleshooting In this chapter we have listed some solutions to various
problems. If you can not solve your problem, contact ZEISS
support.
Disposal Information on the disposal of the product.
1.2 Text Conventions and Link Types
Example Explanation
The names of controls and important infor-
mation are shown in bold letters, for in-
stance:
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 5

1 About this Instruction Manual | 1.3 Explanation of Warning Messages and Additional Infor-
mation ZEISS
Example Explanation
Click Start. Software controls and GUI elements.
Press the Standby button. Hardware controls and elements.
Press Enter on the keyboard. Key on the keyboard.
Press Ctrl+Alt+Del. Press several keys on the keyboard simultane-
ously.
Select Tools>Goto Control Panel>Air-
lock.
Follow a path in the software.
Enter example.pdf in this field. Text to be entered by the user.
Programming and Macros Anything typed in literally during program-
ming, including, for example, macro codes,
keywords, data types, method names, vari-
ables, class names, and interface names.
Tab.1: Text conventions
Example Explanation
See: Text Conventions and Link Types [ } 5].Link to further information for this topic.
https://www.zeiss.com/corporate/int/
home.html
Link to a website on the internet.
Tab.2: Link types
1.3 Explanation of Warning Messages and Additional Information
CAUTION, and NOTICE are standard signal words used to determine the levels of hazards and
risks of personal injury and property damage. Not only the safety and warning messages in the
Safety chapter are to be considered also all safety and warning messages in other chapters. Fail-
ure to comply with these instructions and warnings can result in both personal injury and property
damage and involve the loss of any claims for damages.
The following warning messages indicating dangerous situations and hazards are used in this doc-
ument.
CAUTION
Type and source of danger
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury.
NOTICE
Type and source of danger
NOTICE indicates a potentially harmful situation which, if not avoided, may result in property
damage. In addition, NOTICE warns of data loss or corrupt data as well.
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 6

1 About this Instruction Manual | 1.3 Explanation of Warning Messages and Additional Infor-
mation ZEISS
Info
Provides additional information or explanations to help operator better understand the con-
tents of this document.
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 7

2 Safety | 2.1 Intended Purpose ZEISS
2 Safety
2.1 Intended Purpose
The product is a high definition microscope camera for color imaging. It is suitable for use as an
accessory for educational and routine microscopy in laboratory environments and for use by
trained laboratory personnel. The camera has been designed to be used in the field of light mi-
croscopy for general observation, routine work, and simple applications in which a sufficient
amount of light is available.
The camera should only be used for training and research. The images / videos from this camera
must not be used for the direct generation of diagnostic results.
2.2 General Safety Information
This document must be read before commissioning in order to ensure safe and uninterrupted op-
eration. Pay particular attention to all listed safety notes. Make sure, that
§the operating personnel has read and understood this manual, associated documents, and
particularly all safety regulations and instructions, and applies them.
§the local and national safety and accident prevention regulations must be observed, as well as
the applicable laws and regulations in your country.
§this document is always available at the place of use of the product.
§the product is always in perfect condition.
§the product is secured against access by unauthorized persons.
§maintenance and repair work, retrofitting, removal or replacement of components, as well as
any other intervention in the product not described in this document, may only be carried out
by the manufacturer ZEISS or persons expressly authorized by ZEISS to do so.
2.2.1 Requirements for Operators
The product, components, and accessories may only be operated and maintained by authorized
and trained personnel. The product may only be used in accordance with this document. If the
product is not used as described, the safety of the user may be impaired and/or the product may
be damaged.
Any unauthorized intervention or use other than within the scope of the intended use shall void
all rights to warranty claims. The regional regulations on health protection and accident preven-
tion must be observed at all times and during all work on and with the product.
2.2.2 Safe Operating Condition
If circumstances occur which impair safety and cause changes in operating behavior, the product
must be shut down immediately and a ZEISS service representative should be informed.
The product may only be operated if the operating conditions are adhered to.
§Do not operate the product until you have completely read and understood the entire docu-
mentation.
§Ensure conditions and take measures to prevent the build up of electrostatic charge on the
workplace.
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 8

2 Safety | 2.3 Prevention of Hazards ZEISS
2.3 Prevention of Hazards
This product has been manufactured and tested by ZEISS according to the regulations specified in
CE and has left the manufacturer’s premises in perfect working order. The camera is intended to
be used in a basic electromagnetic environment while the noise emission conforms to CISPR 11
Class B level. The EMC performance might be impaired while integrated into a system. In order to
ensure that this condition is maintained and to avoid any risks when operating the system, the
user must comply with any notes and warnings contained in this manual. The manufacturer shall
be exempt from statutory liability for accidents should the operator fail to observe the safety regu-
lations.
CAUTION
Personal Injury
To avoid personal injury, read and adhere to the safety notes below.
4To avoid the risk of fire or explosion, do not use the camera near inflammable liquids or
gases.
4Setup, expansions, re-adjustments, alterations, and repairs must be carried out only by
persons who have been authorized by ZEISS.
4Do not allow any cables, particularly power cords, to trail across the floor, where they can
be snagged by people walking past.
4Protect the cables from excessive heat (e.g. halogen lamps, microscope fluorescence illu-
mination).
4To avoid injuries due to potentially high surface temperatures, do not touch the camera's
surface for a prolonged time.
4Do not position the equipment in a way that makes it difficult to operate or disconnect the
device.
NOTICE
To avoid equipment damage, data loss, or corrupted data, read and adhere to the safety notes
below.
4Protect the camera against mechanical impact. External damage may affect the operation
of inner components.
4Keep chemicals and fluids away from the camera.
4Make sure there is sufficient ventilation of the camera head. Avoid direct exposure to sun-
light and locations near heat sources (radiators, stoves). Overheating can cause noisy im-
ages.
4Use the camera in a clean and dry location.
4Attach all connectors firmly and securely.
4Use only the accessories supplied by ZEISS, when applicable.
4Use only normal microscope cleaning material to clean the camera housing.
4Contact your local ZEISS service organization if a repair is necessary. Do not disassemble
the camera by yourself, otherwise the warranty will be lost.
4Save all your data, such as images, measurement data, archives, reports, forms and docu-
ments, at regular intervals on an external storage medium. Otherwise it cannot be avoided
that access to this data may be lost as a result of operational errors or hardware defects.
ZEISS accepts no liability for consequential damage resulting from insufficient data protec-
tion.
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 9

3 Technical Data and Conformity | 3.1 Axiocam 105 color R2 ZEISS
3 Technical Data and Conformity
3.1 Axiocam 105 color R2
Feature Value
Sensor Type CMOS color sensor
Number of Pixels 2592 px x 1944 px (horizontal x vertical) = 5 megapixels
Pixel Size 2.2 μm x 2.2 μm
Chip Size 5.70 mm x 4.28 mm, corresponds to 1/2.24"
Live Image Frame Rate 2592 px x 1944 px (horizontal x vertical)
Frame rate @1 ms: 30 fps
Sensor Subarea Readout
(ROI)
Freely adjustable
Min. size: 864 px x 1 px (horizontal x vertical)
Digitization 8 Bit / pixel
Exposure Time 30 μs up to 1 s
Interface (camera) USB 3.0 Micro-B
Interface (PC / card) USB 3.0 Standard-A
Optical Interface C-Mount adapter
Dimensions 29 mm x 29 mm x 17.0 mm (height x width x depth)
Operating System Microsoft® Windows 10
Certificates CE, FCC Class B, RoHS
Power Supply Via USB 3.0 interface
USB Power 5 V, max. 400 mA
Environment Conditions 0° ... + 50° Celsius, max. 80% relative air humidity, no con-
densation, free air circulation required
Order Number 426555-9010-000
Info
Computer hardware, operating system and software may decrease the frame rates. Selecting a
part of the sensor area or applying binning can increase the frame rate. All specifications are
subject to change without notice.
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 10

3 Technical Data and Conformity | 3.2 Applicable Standards and Regulations ZEISS
3.1.1 Spectral Sensitivity
Fig.1: Spectral Sensitivity Axiocam 105 color R2
3.2 Applicable Standards and Regulations
Observe all general and country-specific safety regulations as well as applicable environmental
protection laws and regulations. This product is in compliance with the requirements of the fol-
lowing regulations and directives:
2014/30/EU Electromagnetic Compatibility
2011/65/EU and amendment directive (EU) 2015/863 - RoHS -
Restriction of the use of certain hazardous substances in
electrical and electronic equipment
CE The product fulfills the standards and regulations mentioned
above and is compliant to the CE guideline
For more information on CE Declarations of Conformity, contact your ZEISS Sales & Service Part-
ner.
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 11

4 Shipment ZEISS
4 Shipment
Content Ord. no
1 x Axiocam 105 color R2 camera 426555-9010-000
1 x USB 3.0 data cable
1 x Download information for ZEISS software and instruction
manual
Optional accessories (recommended): PCI express interface card 000000-0591-463
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 12

5 Connecting the Camera | 5.1 Installing the interface card ZEISS
5 Connecting the Camera
5.1 Installing the interface card
This chapter describes how to install the PCI express interface card (2 x USB 3.0) into your PC.
NOTICE
Static Electricity
Static electricity can damage electronic components. To protect electronic components against
static electricity, do not touch them until you have grounded yourself to the casing of the de-
vice. Never touch the contacts of the electronic components. We also recommend that you
work only on an antistatic mat.
Prerequisite üYou have read the documentation of the interface card and of your computer before inserting
the interface card.
1. Switch off your PC and all connected peripherals.
2. Disconnect the PC and the peripherals from the mains and open the computer case.
3. Insert the PCIe interface card into the appropriate slot on your computer.
4. Close the computer case and reconnect the peripherals.
You can now mount the camera on the microscope and connect it to the PC, see chapter Con-
necting the camera to the PC.
5.2 Mounting the camera on the microsope
To mount the camera onto your microscope’s camera port, use a C-mount adapter. NOTICE!The
adapter is not included in the scope of delivery. You will find some suitable examples for
adapters in the list below:
Camera Adapter Description
Camera Adapter 60N-C 2/3" 0.5x 426112-0000-000
Camera Adapter 60N-C 2/3" 0.63x 426113-0000-000
Camera Adapter 95P-C 1/2" 0.5x 415501-1811-000
Camera Adapter 95P-C 2/3" 0.65x 415501-1810-000
1. Remove the dust cap from the camera's C-mount port.
2. Mount the C-mount camera adapter to the camera.
3. Attach the camera with the adapter to the microscope's 60N (P95) port.
4. Orient the camera to the stand and fix its position by tightening the ring nut.
5.3 Connecting the camera with PC
1. Lay the supplied USB 3.0 cable carefully between the camera and the PC.
NOTICE!Make sure that the cable is in a safe distance from hot light sources on
the microscope, to prevent damage from heat.
2. Connect the USB 3.0 cable to the camera. Tighten the screws with a screwdriver until it is
finger tight.
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5 Connecting the Camera | 5.4 Function indicator ZEISS
3. Connect the other end of the USB 3.0 cable to the USB 3.0 interface of the main board
(and not USB 2.0) or the recommened USB 3.0 PCIe interface card.
4. Switch on your PC if its not switched on already. The camera is ready for use.
Info
Power is supplied to the camera via the USB 3.0 interface. If you switch off the PC, the camera
is also switched off.
5.4 Function indicator
After you have switched on the PC, the LED on the camera will light up red. As a rule, if the LED is
red, this indicates that power is being properly supplied to the camera via the data cable. How-
ever the driver has not yet been installed. For the definitions of the LED color signals, refer to the
list below:
Signal Meaning
Red Power supplied; no driver loaded, camera not detected
Green flash 3x USB 3.0 connection detected
Green Driver loaded; camera ready
Off No power supplied
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 14

6 Installing Software and Drivers ZEISS
6 Installing Software and Drivers
Prerequisite üTo install the software for image acquisition, you must install the ZEISS Microscopy Installer.
The camera drivers will be installed automatically during the installation of the software.
üBefore starting the installation of the software and drivers, the camera must be connected to
the PC.
1. Install the software according to the installation guide delivered with the software.
2. During the installation you will be asked to install the camera drivers. Follow the procedure
by confirming the upcoming messages.
3. Restart your PC after the installation.
4. You can check in the device manager if the driver installation was successful.
You can now start acquiring images with your camera using ZEISS software. For more informa-
tion, please read the software documentation.
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7 Care and Maintenance | 7.1 Optical System ZEISS
7 Care and Maintenance
7.1 Optical System
The internal optical components of the camera should always be protected. If no lens, or camera
adapter with optics, is screwed into the camera's C-Mount thread, the camera's sensor and pro-
tective glass must be protected by screwing the protective cap onto the camera's C-Mount
thread.
7.2 Cleaning the infrared filter or protective glass
Contamination of the IR filter (for color cameras only) or the protective glass has an adverse effect
on the quality of the resulting image (dark points, cloudy structures in the image). If there is dry
dust on the front side of the infrared filter or protective glass, you can clean it with a soft brush or
with cotton (wool). Use cleaning fluid for optics/lenses only to clean the IR filter. Do not use tap
water to clean the IR filter.
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8 Troubleshooting | 8.1 The camera does not appear in the menu of selectable cameras ZEISS
8 Troubleshooting
8.1 The camera does not appear in the menu of selectable cameras
§Make sure that you have connected the camera and installed the software and drivers in ac-
cordance to the instructions in this manual.
§Make sure that you installed the software and drivers with administrative rights. Install the
driver with administrative rights again if necessary.
8.2 You don't see a camera image on your screen
§Check if you have started ZEN and clicked the live image button.
§Check the light path setting of the microscope.
§Is the status LED on the camera on? If not, check the cable connections between camera and
computer.
§Reset the camera parameters to default values.
§Execute an automatic exposure measurement.
§Check the display adjustments for the live image.
§Check the aperture diaphragm of the microscope.
§Check the position of the beam splitter between the ocular and the camera port.
For operating the camera with the software, see ZEN Help. Press the F1 key to access the context-
sensitive ZEN Help.
8.3 The color of my image does not correspond to the impression through the
ocular
In case of color cameras:
§Check white balance and, if necessary, repeat white balance.
§Check the monitor’s color temperature setting. If necessary, reduce this to the lowest value
that can be set (usually 5200 K).
In case of monochrome cameras:
§Images of monochrome cameras are displayed by using overlay colors to represent the colors
of fluorescence dyes on the monitor. If the color is different to the visual impression in the
eyepiece, select a different overlay color.
8.4 The performance of the camera is not consistent or is too slow
§Generally the performance of the camera is dependent on the performance of the PC. Make
sure the PC hardware and setup are optimal for large data transfers.
§Check that the camera is connected to the USB 3.0 interface of the main board (and not USB
2.0) or the recommened USB 3.0 interface card on the PC.
§Make sure no other devices are plugged into the USB 3.0 interface card. This can potentially
reduce the speed of the USB 3.0 port.
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9 Disposal ZEISS
9 Disposal
The product must not be disposed of as domestic waste or through municipal disposal compa-
nies. They must be disposed of in accordance with applicable regulations (WEEE Directive
2012/19/EU). ZEISS has implemented a system for the return and recycling of devices in member
states of the European Union that ensures suitable reuse according to the EU Directives men-
tioned. The customer is responsible for decontamination.
Info
Detailed information on disposal and recycling is available from your ZEISS Sales & Service Part-
ner.
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001 18

Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
phone: [phone]
fax: [phone]
[email protected]
www.zeiss.com/microscopy
Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | Modifications reserved.