Aber OPTURASPY User manual

OPTURASPY
Quick start guide
SPY sensor
OPTURASPY Hub
Verification
Standard
USB cable
Vessel Adaptors
Power Supply
USB
Contents:

Using the OPTURASPY
with OPTURAVIEW
Install Software
1. Install OpturaView on host PC.
*Ensure that the device is not connected to PC during
installation
System requirements:
• Microsoft Windows 8.1 or higher
• Minimum of 4 GB of RAM
• Minimum 10 GB free hard disk space
• 1280 x 720 resolution (or higher) display
• Available USB communications port
• Microsoft Office for viewing collected data
Components
2. Check system components:
• OPTURA Spy Base Unit
• Power Supply Unit
• USB Data Cable
• Optura Spy Sensor
• Vessel Adaptors
• Optura Spy Sensor Verification Standard
Connecting your system
3. Connect the power supply unit to the appropriate terminal
on the Optura Spy Hub
4. To connect the sensor to the Optura Spy Hub, remove the
protective dust caps from the connector end of the sensor
and the connector on the base unit; Keep dust caps safe.
5. Push the connector into the base unit ports on the front
of the unit until they have clicked into place. Ensure that
the connector face is not touched in the process.
6. Connect your Optura Spy Hub to the host PC via USB
7. Open OPTURAVIEW and click <Scan for Devices>
8. Select devices, assign a description and check <Include>
and click <OK>
9. All connected devices will be displayed in individual tabs
displaying the OPTURA Hub serial number and assigned
description.
Using a system for the first time
When using the system for the first time it is important to
set the probe type within the system as this will set the
correct sensor coefficients and Bubble Filter Algorithm.
1. Under the device you wish to set up, Go to the
<Measurement setup> tab. Select <Install New Sensor>.
2. In the pop-up window, enter the serial number of the
sensor which can be found on the sticker on the sensor
cable and any description you wish to give the sensor.
3. Select the appropriate sensor type which can be found on
a sticker on the sensor.
4. Select the Bubble Filter to use, this is based on what type
of vessel you are using or the vessel wall thickness. Once
you have selected the appropriate Bubble Filter,
select <OK> to apply these settings. If you are
unsure of which Bubble filter to use, contact
[email protected].
System Checks
1. Under the device you wish to set up, Go to the
<Measurement Setup> tab and select <Sensor
Verication Check> A pop up window for system
verification will appear.
2. Before connecting the SPY sensor to the verification
standard, ensure that no debris is present on the sensor
face.
3. In OPTURAVIEW, follow the guide on the pop up window,
connecting the sensor to the respective high and low
connectors on the verification standard.
System Care
1. Always replace dust caps on the optical terminals
on the base unit, the sensor connector end and the
measurement face when not in use.
2. To clean the measurement face, use compressed air
to remove any light dust/debris. If there are heavier
contaminants, use IPA and a lint free wipe to gently wipe
the surface.
3. To clean the optical connectors, use only compressed
air on both the sensor and the base unit connectors to
remove any dust/debris, do not touch these connectors.
4. When removing the sensor from the vessel adaptor,
ensure to pull the collar away from the vessel adaptor to
disengage the sensor.
5. Never bend the sensor cable beyond the minimum bend
radius of 60 mm.
6. When the system is taking readings, do not unplug the
sensor or move the sensor cable.
7. Ensure that a new vessel adaptor is used for each run.
www.aberinstruments.comOPTURA SPY Quick start guide

Starting your run with
cells
1. Once your vessel/bioreactor is filled
with cell free media, the vessel
adaptor must be attached to the
vessel.
N.B. The OPTURASPY must only be
used on optically clear vessels with
a wall thickness <4.3 mm
2. Flex the wings on the adaptor
before use using your thumbs to
gently work these back and forth.
3. Peel off the sticker from the back
of the adaptor and place firmly
onto your vessel in an area which
has 2.5 cm of free, unimpeded
space inside the vessel. Apply
pressure for 10 seconds.
4. Insert the OPTURASPY sensor
by removing the dust cap and
inserting the sensor into the
adaptor until you hear a click, the
sensor is keyed to be inserted in
one orientation.
5. Open the OPTURAVIEW software as
described above and connect your
OPTURASPY.
6. In OPTURAVIEW, select the tab
<Live Readings>, click <Start
Recording…> and enter your
own unique run ID, confirm the
logging interval, serial number and
whether the system checks have
been performed. Select <OK> to
start recording.
7. Your OPTURA system is now
recording.
8. When the readings are stable in
cell free media click <Add Event>
and select <Zero> from the
drop down and then <OK>. This
eliminates background readings
from your measurements.
N.B. All process conditions should be
set and stable when performing
the zero. Zeroing should be the
last action performed prior to
inoculation.
9. Monitor live trend for a couple
of minutes to verify that zeroed
reflectance readings are stable and
re-zero if necessary.
10. Inoculate your bioreactor.
11. The software should now trace the
growth profile of your process.
12. Click <Stop Recording> only when
you have completed your run.
N.B. If you are calibrating reflectance
readings to offline readings,
follow the Biomass Calibration
instructions in the manual
Flex the wings gently
1
2
3
4
Attaching the
vessel adaptor
Remove the sticker
Attach the vessel
adaptor to the vessel
Use your thumbs to apply
rm pressure to the wings
for 5 seconds
Document part & Issue number : 8500-55-00
OPTURA SPY Quick start guide