Accuris isoPURE Mini User manual

Operation Manual
V1.2
AP1016

Thank you for purchasing the IsoPure™ Mini Automated Purification System. This
user manual details the instrument’s features, specifications, as well as complete
operating instructions; please read it carefully before operation. Keep this user
manual for later use.
Important:
Please keep the box and packaging material for this instrument. If service is
required, the box will be needed to ship the instrument to our Service
Department.
Initial Inspection
Please inspect the instrument as well as all included accessories when you first
open the packaging. If you find anything damaged or missing, please contact
Benchmark Scientific or your local distributor immediately.
BENCHMARK SCIENTIFIC / ACCURIS
INSTRUMENTS
PO Box 709
Edison, NJ 08818
USA
Phone: [phone]
Website: www.benchmarkscientific.com
/ www.accuris-usa.com
Email: [email protected] /
[e-mail]

Safety Warnings and Guidelines
1. Important information for safe use
Users should understand how to use this instrument before operating. Please
read this manual carefully prior to operation.
Any improper operation may cause injury. Please read this manual
carefully and operate safely according to the guidelines.
2. Operation and Maintenance
The operation and maintenance of the instrument should comply with the basic
guidelines and warnings below. Incorrect operation or maintenance will have
detrimental effects on the life, performance, and safety features of the instrument.
The instrument is a normal indoor instrument which conforms to
classⅠof the GB 4793.1 standard.
This instrument is designed for use in a laboratory
environment. The device must be operated by skilled
laboratory personnel with appropriate training.
To prevent injury or voiding the warranty, the operator should not
attempt to repair the instrument without explicit guidance from
Accuris Instruments. If service is required, please contact Accuris
Instruments or your local distributor for repair.
Before powering on, confirm that the voltage used meets the electrical
requirements of the instrument as stated on the rating plate. If the
electric cord is damaged, replace it with the same type of cord. Hold the
socket firmly before pulling the plug from an outlet. Do not pull the
electric cord.
The temperature of the heating block can be high; please do not touch
it while it is in use to prevent injury.
The instrument should be installed in an environment of standard room
temperature, low dust, low humidity, and away from direct sunlight,
electromagnetic interference, and heat sources. Do not block the vents
on the instrument.
Always power off the instrument when you are finished using it. Unplug
the power cord and cover the instrument with a cloth or plastic sheet to
prevent excessive dust from entering the housing.

Pull the connector plug from the electrical outlet immediately and
contact the vendor in the event of:
•Liquid entering the housing.
•Abnormal operation: such as any abnormal sound or smell.
•The instrument is dropped or there is any damage to the
housing.
•Any malfunction.
3. Maintenance
The instrument should be cleaned regularly using a soft cloth dampened with a small
amount of alcohol.
4. Transportation and storage requirements
Ambient temperature: 10℃ ~ 35℃
Relative humidity: ≤ 80%
Atmosphere pressure range: 500 ~ 1060hpa
Place in a well-ventilated room, away from corrosive gas.

Table of Contents
Chapter 1 Introduction..............................................................................................................1
1. Key Features ............................................................................................................................1
Chapter 2 Specifications ..........................................................................................................2
1. Required Installation Environment ...........................................................................................2
2. Specifications ...........................................................................................................................2
3. Dimensions...............................................................................................................................3
Chapter 3 Instrument Overview...............................................................................................4
1. Structure ...................................................................................................................................4
2. Control Panel............................................................................................................................6
Chapter 4 Instrument Setup .....................................................................................................7
1. Preparation Before Use............................................................................................................7
2. 96 Deep Well Plate Installation ................................................................................................8
3. Tip Comb Installation................................................................................................................9
4. Removing Tip Combs...............................................................................................................9
Chapter 5 Programming & Operation....................................................................................10
1. Android App Installation..........................................................................................................10
2. App Main Interface .................................................................................................................11
3. Protocol Select & Transmit Interface......................................................................................11
4. Create New Protocol Interface ...............................................................................................20
5. Acquire Protocol From Instrument Interface ..........................................................................21
6. Instrument Bluetooth Connection Interface............................................................................23
7. Instrument Software Interface ................................................................................................25
Chapter 6 Troubleshooting.....................................................................................................32

1
Chapter 1 Introduction
The IsoPure™ Mini is an automated purification system for DNA/RNA, proteins, and
cells. It can bind, transfer and release magnetic beads by using moveable magnetic
rods. Users can extract 1~16 samples simultaneously with appropriate magnetic bead
kits and reagents available from many suppliers. The IsoPure™ series can be used
with samples from animal/plant tissue, blood, and body fluids.
1. Key Features
➢User-Friendly Input & Operation - Touch screen, 3 operation shortcut keys,
included mouse.
➢Android™ app and PC software included for programming.
➢Internal LED Lighting for visualization of sample processing chamber
➢Open software system – Editable protocols with storage for up to 100
protocols.
➢Heating function – Heating strips for lysis and elution.
➢UV Sterilization function (3W UVC bulb) to reduce risk of cross-contamination.
➢Safe and reliable - Fully automated with disposable consumables.
➢Fast extraction - 10 ~ 60min depending on protocol & optimization.

2
Chapter 2 Specifications
1. Required Installation Environment
Environmental Temperature: 10°C～40°C
Relative Humidity: ≤ 80%
Input Voltage: AC 100～240V, 50Hz/60Hz
2. Specifications
Model
IsoPure™ Mini
Display
4.3-inch touch screen
Purification Method
Magnetic Bead-Based Purification
Sample Volume
50μL—1000μL
Throughput
Up to 16 samples using deep well plates or the
included aluminum rack
Stability
CV ≤ 3%
Extraction Time
10 ~ 60min
Lysis & Elution
Temperature
Ambient +5°C – 120°C
Temperature Accuracy
± 1°C
Programming Control
Equipped with an Android app software to
create, edit and transfer protocols to the
instrument
Program Storage
Store up to 100 protocols in internal memory
Communication Ports
USB for mouse & flash drive
RS232 port for QR code scanning system
Data Transfer
Bluetooth, USB, QR code Scanner
QR Code Scanning
System
Handheld scanner for instant protocol transfer
Consumables
Standard 96 deep well plates, Magnetic rod tip
combs, Aluminum rack, 6-well sample strips

3
UV Sterilization
UV Lamp (3W UVC, 253.7 λ)
Magnetic Bead Recovery
> 95%
Internal Lighting
LED lamp
Power
Standby 10W, MAX 150W
Dimensions
20.8cm x 25.8cm x 31.5cm
Weight
7 kg / 15.4 lbs
3. Dimensions
Fig 1. Dimensions
Dimensions (W×D×H): 7.9x10.2x11.8 in / 208×258×315mm
258mm / 10.2 in
315mm / 11.8 in
208mm / 7.9 in

4
Chapter 3 Instrument Overview
1. Structure
Front
Back
The IsoPure™ Mini is a newly launched automated extraction and
purification system for DNA/RNA, proteins, and cells. It can absorb,
transfer, and release magnetic beads via magnetic rods. Users can
extract 1~16 samples simultaneously with a 96-Deepwell plate. It can
extract samples of animal/plant tissue, blood, and body fluids, etc. with
different kinds of magnetic bead nucleic acid extraction reagents. Users
can extract 1~16 samples
simultaneously with a 96-Deepwell
Operational LED
Indicator
Chamber
Door
Power Switch
Fan
Power Socket Fuse
Port For QR code
Scanning System
Fig 3. Back
Fig 2. Front

5
Side
Fig 4. Side
Sample Processing Chamber
Fig 5. Sample Processing Chamber
PC Port
USB Port
Fuse
UV lamp
Cooling fan (underneath
heating block)
LED lamp (top of processing
chamber)
Magnetic rod
assembly
housing
Tip Comb
holder
Tip Combs
96 deep well
plate

6
2. Control Panel
Fig 6. Touch screen control
panel
Touch screen: For operational control (mouse also can be connected for operation).
TAB: Switch between protocols.
RUN: Start the protocol.
STOP: Stop the protocol.
RUN
TAB
RUN
STOP
4.3-inch touch
screen

7
Chapter 4 Instrument Setup
1. Preparation before use
Remove the instrument from the shipping box. To release the horizontal motor, use
the included Allen key to unscrew the M6 hexagonal screws found underneath the
instrument. Remove the tape holding the chamber door to open the door. Remove the
zip ties between the magnetic rod assembly and the tip comb holder.
Fig 7. Remove the M6 screws
Note: Do NOT power the instrument on prior to removing the M6 hexagonal
screws and zip ties. Please be careful with the magnetic rods when removing
the M5 screws to prevent damage.
Fig 8. Remove the zip ties

8
2. Plate Installation
96 Deep Well Plate Installation
Open the chamber door. First, place the plate in the position according to the blue
arrow ①. Then press the plate into the heating block according to the arrow ②. Note
the orientation of the plate (plate corner A1 should correspond with the mark A1 on the
base of the processing chamber) to avoid the wrong sequence of reagents. After the
plate is in place, close the door.
Fig 9. 96 Deep well plate installation
Aluminum Sample Rack Installation
The Aluminum Sample Rack is inserted in the same way as deep well plates. The
difference is that the Aluminum Sample Rack can process 1~8 samples using 6-well
sample strips.
Fig 10. Aluminum Sample Rack installation
96 deep well plate
Aluminum Sample
Rack
6-well sample
strips

9
3. Tip Comb Installation
Slide the tip combs into the grooves of the tip comb holder following the arrow in Fig.
11.
Fig 11. Tip comb installation
4. Removing Tip Combs
Press down the tab found on the tip comb’s handle according to the blue arrow ①.
While pressing down the tab, pull the tip combs in the direction of the blue arrow ②.
Fig 12. Removing tip combs
Tip comb

10
Chapter 5 Programming & Operation
Protocol programming is done using the Android App software which is saved on the
included USB flash drive. Protocols are then transferred to the instrument where they
are saved in the internal memory and can be selected for operation. For users that do
not have an Android device, PC software can also be used for protocol programming.
The PC software is also saved on the included USB flash drive.
1. Android App Installation:
Connect the included USB flash drive and the Android device to a computer. On the
Android device: Please make sure that downloading from unknown sources is allowed
on your “Files” app setting (Settings→Apps & Notifications→Advanced→Special
App Access→ Install Unknown Apps→Files→ Grant permission), and that “File
Transfer” is selected under “USB Preferences” in the device’s settings
(Settings→Connected Devices→USB→Use USB for “File Transfer”). Copy
the .APK file from the USB flash drive and transfer it into the downloads folder under
the internal storage of the Android device. On the Android device, find the .APK file in
the ”Files” app under “Downloads”, and click the .APK file to install (Fig. 13).
Fig 13. IsoPure™ Mini App Location
Fig 14. App installation

11
2. App Main Interface
After the app has been installed and the startup has completed, the main interface will
be displayed. Four main functions are available: Protocol select and transmit,
Create new protocol, Acquire protocol from instrument, and Instrument
Bluetooth Connection.
3. Protocol Select and Transmit Interface
Click the “Protocol select and transmit” button on the main interface. This interface
offers options for local creation and instrument acquisition (Fig. 16).
Local Creation: Shows a list of protocols saved in the memory on the Android device.
Or click the “ “ icon to create and edit a new protocol. Protocols can be selected
for modification.
Icon
Name
Definition
Protocol
select and
transmit
Quickly select a
protocol for viewing
or transmitting to the
IsoPure Mini
Create new
protocol
Create and edit a
new protocol
Acquire
protocol
from
instrument
Download protocols
from IsoPure Mini
onto app
Instrument
Bluetooth
connection
Connect the Android
device to the IsoPure
Mini via Bluetooth
Fig 15. App main interface
Table 1. App Main Interface Legend

12
Instrument Acquisition: Shows a list of protocols saved in the internal memory of
the instrument. Protocols can be selected for transmission to the app.
Local Creation:
Select a protocol or multiple protocols (Fig. 17, Fig. 18). 4 buttons will appear at the
bottom of the screen: Transmit, Delete, Display QR Code, and Save as.
Fig 17. One Protocol
selected
Fig 18. Multiple protocols
selected
Fig. 16: Protocol Select and
Transmit Interface

13
Transmit: Transfer the selected protocols from the app to the instrument.
Delete: Remove protocols from app memory.
Display QR Code: Generate the protocol specific QR code. Use the included
handheld scanner to instantly transfer the protocol to the instrument. Press “Save”
when the code is displayed to save the code to the photo gallery on the Android device
(Fig. 19).
Save as: The selected protocol can be saved under a new name
Note: “Display QR Code” and “Save as” buttons can only be used when only 1
protocol is selected. When multiple protocols are selected, only Transmit and
Delete can be performed.
Fig 19. QR Codes

14
Preview: Select the blue “Preview” button to the right of a protocol to see the protocol
steps with details (Fig. 20).
New protocol
Click the "New" icon on the “Protocol select and transmit” interface to create a new
protocol (Fig. 21). Set the name of the protocol. The default protocol name is the
current time. For example, the current time is 14:46, so the first protocol is 14461; a
second protocol created within the same minute would be named 14462.
Fig 21. Create & name a new protocol
Fig. 20: Preview of protocol steps