Agilent Technologies InfinityLab G4237A User manual

Agilent Technologies
Agilent InfinityLab Quick
Change Valve G4237A
Instructions
This technical note describes the installation and application of the Agilent
InfinityLab Quick Change 4 column selector Valve Head G4237A in a 1260/1290
Infinity II Multicolumn Thermostat (MCT).
Contents
Typical Applications 2
Multicolumn Selection 2
Method Development 3
Delivery Checklist 3
Install the Valve Heads 6
Remove the Transportation Lock and the Valve Dummy 6
Install the Valve Head and Connect Capillaries 8
Install the Capillaries 12
Setup Examples 14
Valve Specifications 15
Valve Parts 15

2
Typical Applications
Multicolumn Selection
Advantages
•Increase productivity
•Higher instrument up-time
The valve facilitating quick changes allows the selection between up to four
different stationary phases for a variety of applications, or the usage of
identical stationary phases in columns with different dimensions for either
faster run-times (short columns) or higher resolution (long columns) or for
loading studies with different internal diameters.
Figure 1 The G7116A 1260 Infinity II Multicolumn Thermostat equipped with an
InfinityLab Quick Change 4 Column Selector Valve Head

3
Method Development
Advantages:
•Faster method development
•Automated method development possible
Figure 2 Totally different chromatographic results by using the same sample but three
different stationary phases
Delivery Checklist
Check the content of the delivery. You should have received the following:
G4237A:
p/n Description
5067-4279 4 column selector valve head, 800 bar
5067-6596 Cap kit 0.12 mm, 4-col, incl. QC-HE
(Optional)
5067-4300 Cap kit 0.17 mm, 4-col, incl. QC-HE
(Optional)
P$8
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4
The different Capillary Kits for the 4-Column Selector contain the following
parts:
Capillary Kit (5067-6596)
# p/n Description
4 G7116-60015 Quick Connect Heat Exchanger Standard
4 G7116-68003 Column Holder Clips (2/Pk)
1 5500-1202 Capillary ST 0.12 mm x 500 mm M4-SL PS-PS
autosampler to valve
4 5500-1199 Capillary ST 0.12 mm x 130 mm M4-SL PS-PS
valve to heat exchanger
4 5500-1200 Quick Turn Capillary ST 0.12 mm x 130 mm
SL/M
column to valve
1 5063-6591 PEEK Fittings 10/PK
column outlet
4 5500-1201 Capillary ST 0.12 mm x 105 mm SL-- PS-LS
heat exchanger (PS-SL) to column
1 5500-1203 Capillary ST 0.12 mm x 280 mm M4-SL PS-PS
valve to detector
1 5500-1204 Capillary ST 0.12 mm x 150 mm M4-M4 PS-PS
valve to valve (column bypass)
8 G1314-68703 Cap fitting kit special
1 5023-2504 Hex driver SW-4 slitted
1 5067-6141 M4 Blank nut
1 G1375-87326 Waste tube
1 5067-6654 Number Kit 1-8 colored

5
Capillary Kit (5067-4300)
Capillary and Fitting Information
•M4 = fitting thread size for micro valve ports
•PS = pre-swaged fitting
•NS = non-swaged fitting
•SL = fitting screw long
•LS = long capillary socket (required for special fittings as the A-Line Quick
Turn Fittings)
•SV = removable high pressure fitting
# p/n Description
4 G7116-60051 Quick-Connect Heat Exchanger Large ID
4 G7116-68003 Column Holder Clips (2/Pk)
1 5067-6188 Capillary ST 0.17 mm x 500 mm SL-M4 PS-PS
autosampler to valve
4 5067-5109 Capillary ST 0.17 mm x 90 mm SL/M
valve to heat exchanger
4 5067-4746 Capillary ST 0.12 mm x 250 mm SLV/M
long column to valve
4 5500-1200 Quick Turn Capillary ST 0.12 mm x 130 mm SL/M
short column to valve
2 0100-1516 Fingertight fitting PEEK, 2/pk
column outlet
4 5500-1193 Capillary ST 0.17 mm x 105 mm, long socket
heat exchanger to column
1 5500-1203 Capillary ST 0.12 mm x 280 mm M4-SL PS-PS
valve to detector
1 5500-1204 Capillary ST 0.12 mm x 150 mm M4-M4 PS-PS
valve to valve (column bypass)
1 5065-4454 Fitting screw long
10/pk
1 5023-2504 Hex driver SW-4 slitted
1 5067-6141 M4 Blank nut
1 G1375-87326 Waste tube
2 G1314-68703 Cap fitting kit special
1 5067-6654 Number Kit 1-8 colored

6
Install the Valve Heads
The valve drives are factory-installed in the Multicolumn Thermostat. The
valve heads are interchangeable and can be easily mounted.
At the first installation, the transportation lock and the dummy valve have to
be removed, see “Remove the Transportation Lock and the Valve Dummy” on
page 6. The valve heads can be installed by mounting the valve heads onto the
valve drives and fastening the nut manually (do not use any tools).
Be sure that the guide pin snaps into the groove of the valve drive thread.
Remove the Transportation Lock and the Valve Dummy
The following procedure demonstrates the necessary steps for installing the
valve head to the valve drive of a Multicolumn Thermostat (MCT).
For the installation of a valve head to a G1170A Valve Drive you can ignore the
steps that describe the MCT features of the transportation lock and spring
loaded valve drive.
1Switch off the module.
2When unscrewing the transportation lock, push it back until the last screw
is removed - the valve rail is spring-loaded.
NOTE
The valves are mounted on pull-out rails to allow easy installation of
capillaries. Push the valve gently into its housing until it snaps into
the inner position, push it again and it slides out.
When all capillaries are installed, push the valve back into its
housing, see “Install the Valve Head and Connect Capillaries” on
page 8.

7
3Press on the valve dummy (1.) to release it (2.) (spring-loaded valve rail).
4
5
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8
Install the Valve Head and Connect Capillaries
For bio-inert modules use bio-inert parts only!
CAUTION
The valve actuator contains sensitive optical parts, which need to
be protected from dust and other pollution. Pollution of these
parts can impair the accurate selection of valve ports and
therefore bias measurement results.
➔Always install a valve head for operation and storage. For
protecting the actuator, a dummy valve head can be used
instead of a functional valve. Do not touch parts inside the
actuator.
CAUTION
Column Damage or Bias Measurement Results
Switching the valve to a wrong position can damage the column
or bias measurement results.
➔Fit the lobe to the groove to make sure the valve is switched to
the correct position.
CAUTION
Valve Damage
Using a low pressure valve on the high pressure side can damage
the valve.
➔When using multiple column compartments as part of a
method development solution, make sure that the high
pressure valve head is connected to the autosampler and the
low pressure valve head is connected to the detector.

9
CAUTION
Sample degradation and contamination of the instrument
Metal parts in the flow path can interact with the bio-molecules
in the sample leading to sample degradation and contamination.
➔For bio-inert applications, always use dedicated bio-inert parts,
which can be identified by the bio-inert symbol or other
markers described in this manual.
➔Do not mix bio-inert and non-inert modules or parts in a
bio-inert system.
CAUTION
Wrong combination of fitting with valve
The InfinityLab Quick Turn fitting (5067-5966) is not compatible
with the G5639A Bio-inert 4-Column Selector Valve. Misuse can
lead to extra dead volume and leaks.
➔As fitting, use UHP fitting (5067-5403) instead.
NOTE
For information about the compatibility mode of 800 bar valve heads
see Information on RFID Tag Technical Note (01200-90134).
NOTE
For a correct installation of the valve head, the outside pin (red) must
completely fit into the outside groove on the valve drive’s shaft (red).
A correct installation is only possible if the two pins (green and blue)
on the valve head fit into their corresponding grooves on the valve
drive’s actuator axis. Their match depends on the diameter of the pin
and groove.
NOTE
The tag reader reads the valve head properties from the valve head
RFID tag during initialization of the module. Valve properties will not
be updated, if the valve head is replaced while the module is on.
Selection of valve port positions can fail, if the instrument does not
know the properties of the installed valve.
NOTE
To allow correct valve identification, power off the valve drive for at
least 10 s.
NOTE
For firmware requirements see Information on new RFID Tag
Assembly Version Technical Note (01200-90133) which is included to
each valve head.

10
The following procedure shows the valve head installation with an
G7116A/B (MCT) module as an example. For other modules it is similar.
1Insert the valve head into the valve shaft.
OR
If the outside pin does not fit into the outside groove, you have to turn the
valve head until you feel that the two pins snap into the grooves. Now you
should feel additional resistance from the valve drive while continuously
turning the valve head until the pin fits into the groove.

11
2When the outer pin is locked into the groove, manually screw the nut onto
the valve head.
NOTE
Fasten the nut manually. Do not use any tools.
3Install all required capillary connections to the valve.
4Push the valve head until it snaps in and stays in the rear position.
5Power on or power-cycle your module, so the valve head gets recognized
during module initialization.

12
Install the Capillaries
1Install the In and Out connectors.
•from sampler to the valve ( Capillary ST 0.12 mm x 500 mm M4-SL PS-PS
(5500-1202)) or on a standard system (with 0.17 mm capillaries)
Capillary ST 0.17 mm x 500 mm SL-M4 PS-PS (5067-6188)
•from valve to the detector ( Capillary ST 0.12 mm x 280 mm M4-SL PS-PS
(5500-1203))
The In port is hydraulically connected to the column inlet ports 1-4 while
the Out port connects to the column outlet ports 1`-4`.
CAUTION
Damage to the rotor seal
Instant pressure release within the valve will lead to water jet
effects that can harm internal parts of the valve. This pressure
release typically happens if the valve gets switched under high
pressure over unused or open channels.
➔Block all unused channels properly with the M4 blank nut.
NOTE
To minimize valve movement over open connections it is
recommended to plumb the column connected channels in one row.
e.g.:
•channel 1 – column 1
•channel 2 – column 2
•channel 3 – blocked
•channel 4 – waste
NOTE
The blank nuts are only required for the ports on the inner circle that
connect the valve with the column inlet.
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13
2Install the column inlet and outlet connections.
•- ports 1-4 for connections from valve to the heat exchanger ( Capillary
ST 0.12 mm x 130 mm M4-SL PS-PS (5500-1199)) or on a standard system
(with 0.17 mm capillaries) Capillary ST 0.17 mm x 90 mm SL/M
(5067-5109) or waste line (Waste tube (G1375-87326))
•- ports 1`-4` for connections from column outlet to valve, use
fingertight PEEK fittings for connecting the column outlet.
For a short column: use Quick Turn Capillary ST 0.12 mm x 130 mm SL/M
(5500-1200)
For a long column: use Capillary ST 0.12 mm x 250 mm SLV/M
(5067-4746)
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14
Setup Examples
11-4 column-selection
Figure 3 Hydraulic flow path schematics for a 4-column selection setup
2Two column selection with purge line and valve-bypass
Figure 4 Hydraulic flow path schematics for 2-column selection setup with purge
and valve bypass line

15
Valve Specifications
Valve Parts
Replacement Parts
Table 1 G4237A (5067-4279), 4ps/10pt, 4-column selector, 800 bar
Type Specification
Maximum pressure 800 bar
Typical application 4 column selection
Port size Accepts M4 male threaded fittings
Liquid contacts PEEK, Stainless Steel
pH range 0 – 14*
* incompatible with some mineral acids. For more information see Solvent Information.
Table 2 Replacement Parts
Valve Rotor
Seal
Stator
Head
Bearing
Ring
Stator Screws
(Pack of 10)
Stator
Ring
4 column selector
valve head, 800 bar
(5067-4279)
5068-0264 5068-0263 1535-4045 5068-0019 n.a.

*G4237-90010*
*G4237-90010*
G4237-90010
Part Number:
G4237-90010 Rev. B
Edition: 10/2018
Printed in Germany
© Agilent Technologies, Inc
2016-2018
Agilent Technologies, Inc
Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
Valve Head Parts
Figure 5 Valve Head Parts (example)
NOTE
The figure below illustrates replacement parts for the valve heads, with
the 12ps/13pt Selector valve as an example. The valves can vary in
their appearance and do not necessarily include all of the illustrated
parts. Neither, every spare part is available for each flavor of the valve.
1 Stator screws
2 Stator head assembly
3 Stator ring screws (not available)
4 Stator ring (available for service only)
5 Rotor seal
6 Bearing ring
7 Spanner nut (available for service only)
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