Agilent Technologies G1323B User manual

A
Agilent 1200 Series
Handheld Control
Module
User’s Guide

1200 Series Handheld Control Module Users’s Guide
Notices
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Manual Part Number
G1323-90010
Edition
02/2006
Printed in Germany
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1200 Series Handheld Control Module Users’s Guide 3
In This Guide…
This book describes how to operate the Agilent 1200 Series modules and
systems for HPLC using the control module.
Solvent cabinet
Vacuum degasser
Pump
Column compartment
Autosampler
Detector
Control module

4 1200 Series Handheld Control Module Users’s Guide
The control module provides complete local control and monitoring of a single
module or an entire Agilent 1200 Series system. There is no data evaluation in
the control module. The control module allows you to do a variety of HPLC
tasks including automated sample preparation and injection, isocratic,
gradient and multiple method analyses.
Chapter Overview
Part 1 Using the Agilent 1200 Series Control Module
This part describes the control mode, its features and its functionality.
1 The Agilent 1200 Series Control Module
This chapter gives an overview over the Agilent 1200 Series control module.
2 Working with the Control Module
This chapter describes how to use the Agilent 1200 Series control module.
Part 2 Using the Agilent 1200 Series Modules
This part describes how to use the individual HPLC modules to run isocratic,
gradient and multiple-vial analyses using a single method or more than one
method.
3UsingthePump
This chapter contains operational details for the Agilent 1200 Series pumping
systems.
4UsingtheDegasser
This chapter contains operational details for the Agilent 1200 Series vacuum
degasser.
5 Using the Autosampler
This chapter contains operational details for the Agilent 1200 Series
autosampler.

1200 Series Handheld Control Module Users’s Guide 5
6 Using the Manual Injection Valve
This chapter contains operational details for the Agilent 1200 Series manual
injection valve.
7 Using the Detectors
This chapter contains operational details for the Agilent 1200 Series variable
wavelength, multiple wavelength, refractive index, fluorescence light, and
diode array detectors.
8 Using the Column Compartment
This chapter contains operational details for the Agilent 1200 Series
thermostatted column compartment.
Part 3 Using the Agilent 1200 Series LC System With Control Module
This part describes how to run isocratic, gradient and multiple-vial analyses
using a single method or more than one method.
9 Running an Isocratic Analysis
This chapter describes how to analyze the Agilent Technologies isocratic
standard sample using a single injection analysis.
10 Running a Gradient Analysis
This chapter describes how to analyze the Agilent Technologies isocratic
standard sample using a gradient analysis.
11 Running Multiple-Vial Analyses
This chapter descibes how to setup multiple vial analyses using the same
method and different methods.
12 Running an Injector Program
This chapter descibes how to create an injector program.
13 Maintaining the Control Module
This chapter shows the repair items.

6 1200 Series Handheld Control Module Users’s Guide
A Appendix
This chapter contains safety information.
Related Documents
Each HPLC module is supplied with a Reference Manual or User Manual.
The control module is supplied with the following:
• User’s Guide
• Software Overview Guide

1200 Series Handheld Control Module Users’s Guide 7
Contents
1 The Agilent 1200 Series Control Module 15
Control Module Features 16
Control Module Keys 17
The i (info) key - Online Information System 19
The m (menu) key 20
The Esc key 20
The Enter key 21
Navigation keys 21
Action keys 21
Selection keys 21
Direction keys 21
Numeric/Alphanumeric keys 22
Control Module Software 23
The Control Module User Interface 23
Analysis Screen 23
System Screen 27
Status Screen 29
Samples Screen 30
Plot Screen 31
Control Module Versions 32
2 Working with the Control Module 35
Installing the Control Module 36
General Functions 37
Turning On/Off LC System and Modules 37
Setting Date and Time 37

8 1200 Series Handheld Control Module Users’s Guide
Contents
Setting Display Contrast 37
Adjusting View 38
Configuring the LC System 38
Troubleshooting 39
Troubleshooting the Control Module 39
Inserting and Removing PC cards 40
Working with Methods 41
Loading a Method 41
Modifying a Method 42
Specifying a Method Name 42
Protecting a Method 43
Saving a Method 44
Deleting a Method 45
Exchanging Methods With the PC Card 45
Transferring Methods Between LC Systems 46
Time Programming 47
Automating Analyses 48
Displaying Data Graphically 50
Selecting Signals 50
Rescaling the Plot Screen 51
Toggling Signals 52
Displaying Exact Signal Values 52
Print Graph 52
Logbooks 53
Maintenance Logbook 53
Error Logbook 54
System Logbook 54
EMF (Early Maintenance Feedback) 54
Printing Screens 55
Print Plot 55

Contents
1200 Series Handheld Control Module Users’s Guide 9
Print Logbooks 56
Print Method 56
Print Timetable 56
Print Sequence 56
Print Injector Program 56
Connecting External Devices 57
APG Remote 57
GPIB 59
Serial / RS-232 59
MIO 59
BCD 59
External Contacts 59
Firmware Updates 60
Coexecution with Agilent ChemStation 61
Features 61
Restrictions 62
3UsingthePump63
Turning the Pump On and Off 64
Entering Settings 64
Purging the Pump 64
Purging Procedure 65
Adjusting Compressibility 66
Adjusting Stroke Volume 68
Automatic Shut-down 68
Troubleshooting the Pump 68
Tracking Pump History / Pump EMF Limits 69
Resetting the Pump 70

10 1200 Series Handheld Control Module Users’s Guide
Contents
Resetting the Pump Settings 70
4UsingtheDegasser71
Starting the Degasser 72
Removing Gas Bubbles 72
Changing Solvents 73
5 Using the Autosampler 75
Configuring the Autosampler 76
Configuring Interfaces 76
Entering Settings 77
Setting the Injection Mode 77
Optimizing Autosampler Performance 78
Setting up the Thermostatted Autosampler 79
Aligning Transport Arm/Gripper 80
Tracking Autosampler History / EMF limits 81
Resetting the Autosampler 81
Reset to Default Settings 81
Troubleshooting the Autosampler 82
6 Using the Manual Injection Valve 83
About the Injection Valve 84
Making An Injection 85
Completely Filling the Sample Loop 85
Partially Filling the Sample Loop 86
How Much Sample Is Actually Injected? 86
How Much Sample Do I Need? 87
An Alternative Way to Fill The Loop 87

Contents
1200 Series Handheld Control Module Users’s Guide 11
7 Using the Detectors 89
Turning the Lamp On and Off 90
Entering Settings 91
Diode Array Detector 91
Variable Wavelength Detector 91
Fluorescence Light Detector 91
Multiple Wavelength Detector 92
Refractive Index Detector 92
Resetting the Baseline 93
Configuring the Detector 94
Troubleshooting the Detector 95
Tracking Detector History 96
Resetting the Detector 97
Lamp Ignition Routine (VWD only) 98
Configuring the Analog Channel Output 99
8 Using the Column Compartment 101
Turning the Column Compartment On and Off 102
Entering Settings 103
Configuring the Column Compartment 104
Configuring the Column ID Module 105
Selecting Separated or Combined Mode 106
Selecting the Column Switching Valve (optional) 107
Troubleshooting the Column Compartment 108
Tracking Column Compartment History 109
Tracking Column ID History 110
Resetting the Column Compartment 111

12 1200 Series Handheld Control Module Users’s Guide
Contents
9 Running an Isocratic Analysis 113
What You Will Need 114
Preparing the LC System 115
Entering Settings 116
Saving Settings in a Method 118
Observing the Chromatogram 119
Starting the Analysis 121
10 Running a Gradient Analysis 123
What You Will Need 124
Preparing the LC System 125
Entering Settings 126
Saving Settings in a Method 128
Observing the Chromatogram 129
Starting the Analysis 131
11 Running Multiple-Vial Analyses 133
Analyzing Multiple Vials Using the Same Method 134
Analyzing Multiple Vials Using Different Methods 135
Single-Level Calibration Sequences 137
Multiple-Level Calibration Sequences 139
Recalibrating With the Same Group of Standards 139
Recalibrating With Multiple Groups of Standards 141
Synchronizing Analyses with External Devices 144
Standard 144
Send Single Start Request 145
Send Repeated Start Request 146
Wait for Single (External) Start Request 146

Contents
1200 Series Handheld Control Module Users’s Guide 13
Wait for Repeated Start Request 147
12 Running an Injector Program 149
Creating an Injector Program 150
Entering Injector Program Settings 151
Injector Program Lines 152
Saving the Method 153
13 Maintaining the Control Module 155
Control Module Parts 156
A Appendix 157
Safety Information 158
General 158
Operation 158
Safety Symbols 159
Radio Interference 161
Test and Measurement 161
Agilent Technologies on Internet 161
Index 163

14 1200 Series Handheld Control Module Users’s Guide
Contents

15
Agilent 1200 Series Handheld Control Module
User’s Guide
Agilent Technologies
1
The Agilent 1200 Series Control Module
Control Module Features 16
Control Module Keys 17
Control Module Software 23
Control Module Versions 32

16 1200 Series Handheld Control Module Users’s Guide
1 The Agilent 1200 Series Control Module
Control Module Features
Control Module Features
The control module provides complete local control and monitoring of a single
module or an entire Agilent 1200 Series system. You have easy access on every
supported function, you can easily control all parameters and settings and you
can configure various communication channels with other devices, in order to
comfortably analyze the generated data.
•Install any desired configuration of Agilent 1200 Series modules. The
control module software will reflect which modules are present in the LC
system and adjust the screens accordingly.
•Enter parameter settings for every module, perform reset and on/off
functions as well as calibration and configuration settings in a
self-explanatory and intuitive way.
•Define automated analyses including methods, timetables, injector
programs, method sequences and automated calibration settings using the
control module.
•Protect your method from any inadvertent keyboard changes by setting
method protection.
•Use PC cards to store and transfer methods and sequences between
Agilent1200 systems.
•Monitor all operations and error events using the self-updating logbooks.
•Use the context-sensitive online information system to get further
information on all topics.
•Use the context-sensitive menu function to have the quickest access on
related functions.
•To help comply with Good Laboratory Practice (GLP) regulations you can
select a variety of module tests that will check the performance of the LC
system.
•The early maintenance feedback (EMF) limits can be used for scheduling
maintenance work.
•Display data graphically using the Plot screen where as many as three
different signals can be monitored at the same time.
•Print information to a PCL3 compatible printer connected to the serial
RS232 port of a Agilent1200 module.

1200 Series Handheld Control Module Users’s Guide 17
The Agilent 1200 Series Control Module 1
Control Module Keys
Control Module Keys
Figure 1 The Agilent 1200 Series Control Module
i (info) key
provides
context
specific
information for
current item
selected
Enter key
accepts
current input
entry or action
Selection keys
select settings, values or
alphabetic characters
Direction keys
move the cursor in
the screen display
Navigation keys
access different
software areas
Esc key
reverses current
input entry or
action
Numeric keys
enter numbers 0 to 9
Action keys
execute
specific
operations and
access Plot
screen
m (menu) key
provides
navigational
shortcuts and
actions
Alphanumeric keys
press numbers 1 to 9 multiple times
(e.g. 1 A B C, 2 D E F, 3 G H I ,…)
NOTE A short description of the main keys and the product and serial number is located on the
rear of the control module.

18 1200 Series Handheld Control Module Users’s Guide
1 The Agilent 1200 Series Control Module
Control Module Keys
The display will show you a variety of menu buttons [F1-F5] (in the lower
section) or function buttons [F6-F8] (in the right hand section) that can be
accessed with the corresponding Navigation (for menus) and Action (for
functions) keys.
NOTE In this context the expression “button” will always refer to a menu or function shown on
the display, whereas “key” refers to the actual keys on the keyboard. The key
corresponding to a certain button is shown in brackets [F1-F8]

1200 Series Handheld Control Module Users’s Guide 19
The Agilent 1200 Series Control Module 1
Control Module Keys
The i (info) key - Online Information System
The online information system provides a quick and convenient way to look up
information about a task you are doing or a feature or screen you would like to
know more about. The online information system is context-sensitive and
provides information related to the current topic.
You can access the online information system by using the i (info) key on the
control module keyboard.
Some words are underlined which indicates available further information. By
pressing the Enter key you can display screens providing more specific
information about the underlined word.
Some words have a dotted underline which indicates there is an available
definition of the word. By pressing Enter you can display a small overlay screen
with information. You can remove this overlay screen by pressing Enter.
•To exit from the online information system press Esc.
•To find out what other information topics are available select the Search
button [F8]. From there you can choose between Contents and Index.
•To access further screens containing related information on the currently
selected screen select the Related button [F7].
•To move back to the previous information screen select the Previous button
[F6].
Figure 2 Online Information System - Table of Contents

20 1200 Series Handheld Control Module Users’s Guide
1 The Agilent 1200 Series Control Module
Control Module Keys
The m (menu) key
You can use the m(menu) key on the control module keyboard to access
selected functionality quickly wherever you are in the software. For example,
from the Table of Contents you have quick access to the Print function. In
other screens you can easily select the Restart or Default functions or a
schematic diagram for explanation purposes. Press the Esc key to exit the
menu. See the Quick Reference Guide on the availability of context sensitive
menus. In Figure 3 Analysis Screen context menus are shown. Depending on
the active parameter entry field context sensitive menus provide different
options.
The Esc key
The Esc (Escape) key allows you to exit the current window or screen and
leads you back to the last window or screen you were working with.
If you are in one of the main screens, you can use Esc to toggle between the
current and the previous screen.
In an edit field the previous value can be restored by pressing ESC.
Figure 3 Context Menus in the Analysis Screen