Agilent Technologies G1323B User manual

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Agilent 1200 Series
Handheld Control
Module
User’s Guide

1200 Series Handheld Control Module Users’s Guide
Notices
© Agilent Technologies, Inc. 2000-2006
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Manual Part Number
G1323-90010
Edition
02/2006
Printed in Germany
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1200 Series Handheld Control Module Users’s Guide 3
In This Guide…
This book describes how to operate the Agilent 1200 Series modules and
systems for HPLC using the control module.
Solvent cabinet
Vacuum degasser
Pump
Column compartment
Autosampler
Detector
Control module

4 1200 Series Handheld Control Module Users’s Guide
The control module provides complete local control and monitoring of a single
module or an entire Agilent 1200 Series system. There is no data evaluation in
the control module. The control module allows you to do a variety of HPLC
tasks including automated sample preparation and injection, isocratic,
gradient and multiple method analyses.
Chapter Overview
Part 1 Using the Agilent 1200 Series Control Module
This part describes the control mode, its features and its functionality.
1 The Agilent 1200 Series Control Module
This chapter gives an overview over the Agilent 1200 Series control module.
2 Working with the Control Module
This chapter describes how to use the Agilent 1200 Series control module.
Part 2 Using the Agilent 1200 Series Modules
This part describes how to use the individual HPLC modules to run isocratic,
gradient and multiple-vial analyses using a single method or more than one
method.
3UsingthePump
This chapter contains operational details for the Agilent 1200 Series pumping
systems.
4UsingtheDegasser
This chapter contains operational details for the Agilent 1200 Series vacuum
degasser.
5 Using the Autosampler
This chapter contains operational details for the Agilent 1200 Series
autosampler.

1200 Series Handheld Control Module Users’s Guide 5
6 Using the Manual Injection Valve
This chapter contains operational details for the Agilent 1200 Series manual
injection valve.
7 Using the Detectors
This chapter contains operational details for the Agilent 1200 Series variable
wavelength, multiple wavelength, refractive index, fluorescence light, and
diode array detectors.
8 Using the Column Compartment
This chapter contains operational details for the Agilent 1200 Series
thermostatted column compartment.
Part 3 Using the Agilent 1200 Series LC System With Control Module
This part describes how to run isocratic, gradient and multiple-vial analyses
using a single method or more than one method.
9 Running an Isocratic Analysis
This chapter describes how to analyze the Agilent Technologies isocratic
standard sample using a single injection analysis.
10 Running a Gradient Analysis
This chapter describes how to analyze the Agilent Technologies isocratic
standard sample using a gradient analysis.
11 Running Multiple-Vial Analyses
This chapter descibes how to setup multiple vial analyses using the same
method and different methods.
12 Running an Injector Program
This chapter descibes how to create an injector program.
13 Maintaining the Control Module
This chapter shows the repair items.

6 1200 Series Handheld Control Module Users’s Guide
A Appendix
This chapter contains safety information.
Related Documents
Each HPLC module is supplied with a Reference Manual or User Manual.
The control module is supplied with the following:
• User’s Guide
• Software Overview Guide

1200 Series Handheld Control Module Users’s Guide 7
Contents
1 The Agilent 1200 Series Control Module 15
Control Module Features 16
Control Module Keys 17
The i (info) key - Online Information System 19
The m (menu) key 20
The Esc key 20
The Enter key 21
Navigation keys 21
Action keys 21
Selection keys 21
Direction keys 21
Numeric/Alphanumeric keys 22
Control Module Software 23
The Control Module User Interface 23
Analysis Screen 23
System Screen 27
Status Screen 29
Samples Screen 30
Plot Screen 31
Control Module Versions 32
2 Working with the Control Module 35
Installing the Control Module 36
General Functions 37
Turning On/Off LC System and Modules 37
Setting Date and Time 37

8 1200 Series Handheld Control Module Users’s Guide
Contents
Setting Display Contrast 37
Adjusting View 38
Configuring the LC System 38
Troubleshooting 39
Troubleshooting the Control Module 39
Inserting and Removing PC cards 40
Working with Methods 41
Loading a Method 41
Modifying a Method 42
Specifying a Method Name 42
Protecting a Method 43
Saving a Method 44
Deleting a Method 45
Exchanging Methods With the PC Card 45
Transferring Methods Between LC Systems 46
Time Programming 47
Automating Analyses 48
Displaying Data Graphically 50
Selecting Signals 50
Rescaling the Plot Screen 51
Toggling Signals 52
Displaying Exact Signal Values 52
Print Graph 52
Logbooks 53
Maintenance Logbook 53
Error Logbook 54
System Logbook 54
EMF (Early Maintenance Feedback) 54
Printing Screens 55
Print Plot 55

Contents
1200 Series Handheld Control Module Users’s Guide 9
Print Logbooks 56
Print Method 56
Print Timetable 56
Print Sequence 56
Print Injector Program 56
Connecting External Devices 57
APG Remote 57
GPIB 59
Serial / RS-232 59
MIO 59
BCD 59
External Contacts 59
Firmware Updates 60
Coexecution with Agilent ChemStation 61
Features 61
Restrictions 62
3UsingthePump63
Turning the Pump On and Off 64
Entering Settings 64
Purging the Pump 64
Purging Procedure 65
Adjusting Compressibility 66
Adjusting Stroke Volume 68
Automatic Shut-down 68
Troubleshooting the Pump 68
Tracking Pump History / Pump EMF Limits 69
Resetting the Pump 70

10 1200 Series Handheld Control Module Users’s Guide
Contents
Resetting the Pump Settings 70
4UsingtheDegasser71
Starting the Degasser 72
Removing Gas Bubbles 72
Changing Solvents 73
5 Using the Autosampler 75
Configuring the Autosampler 76
Configuring Interfaces 76
Entering Settings 77
Setting the Injection Mode 77
Optimizing Autosampler Performance 78
Setting up the Thermostatted Autosampler 79
Aligning Transport Arm/Gripper 80
Tracking Autosampler History / EMF limits 81
Resetting the Autosampler 81
Reset to Default Settings 81
Troubleshooting the Autosampler 82
6 Using the Manual Injection Valve 83
About the Injection Valve 84
Making An Injection 85
Completely Filling the Sample Loop 85
Partially Filling the Sample Loop 86
How Much Sample Is Actually Injected? 86
How Much Sample Do I Need? 87
An Alternative Way to Fill The Loop 87