Agilent Technologies 55B AA User manual

Agilent 55B AA Spectrometer
User’s Guide

2 Agilent 55B AA Spectrometer User’s Guide
Notices
Manual Part Number
8510154100
Edition 13, February 2019
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Safety Notices
A CAUTION notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in personal injury or death.
Do not proceed beyond a WARNING
notice until the indicated conditions are
fully understood and met.
WARNING
CAUTION

Safety Practices and Hazards
Agilent 55B AA Spectrometer User’s Guide 3
Contents
Contents 3
1Safety Practices and Hazards 11
Verify safe state 12
Electrical hazards 12
Heat, vapors and fumes 13
Compressed gases and cylinders 14
Gas hoses and connections 17
Ultraviolet radiation 18
Other 18
Warning symbols 18
CE compliance 20
Electromagnetic compatibility 21
EN55011/CISPR11 21
ICES/NMB-001 22
Flame operation 22
Flammable solvents 22
Burners 24
Nebulizer 25
Liquid trap 26
Heat hazards 27
Perchloric acid 27
Flashbacks 29
What to do in the event of a flashback 31

Safety Practices and Hazards
4 Agilent 55B AA Spectrometer User’s Guide
2Introduction 33
Site preparation requirements 33
Documentation 34
Conventions 34
Specifications 34
Environmental specifications 34
3Overview 37
Installation checklist 37
Instrument overview 37
Power 39
Gas hoses 39
Printer 39
Accessories 39
Moving your instrument 40
Lamp compartment 40
Hollow cathode lamps 40
Deuterium (D2) lamp 41
Sample compartment 41
Nebulizer block assembly 42
Burner 46
Sample compartment front panel 46
Flame shield 47
Chimney 48
4Interface 49
Front panel overview 49

Safety Practices and Hazards
Agilent 55B AA Spectrometer User’s Guide 5
Display screen 50
Keypad 53
Navigating the interface 56
Changing pages 56
Selecting menus 56
Moving between fields 58
Display pages 58
Load Method page 58
Instrument Parameters page 58
Measurement parameters page 60
Options page 61
Optimization page 63
Calibration Parameters page 64
Results page 64
5Operation 67
Pre-analysis checklist 67
Start the system 69
Method development 69
Select the language of the interface 69
Load a method 70
Save/Delete methods 71
Set the instrument parameters 72
Set the measurement parameters 72
Define the calibration standards 73
Optimization 74

Safety Practices and Hazards
6 Agilent 55B AA Spectrometer User’s Guide
Align lamps 74
Align the burner 76
Light the flame 78
Optimize the flame signal 80
Nebulizer setup 81
High vacuum setting 82
Low vacuum setting 83
Set zero uptake rate – organic solvents 83
Set the uptake rate for organic solvents 84
Flame types 84
Performance checks 85
Calibrate the method 86
Measure standards manually 86
Measure standards using SIPS 10 86
Display the calibration graph 87
Reslope the calibration 87
Sample measurement 88
Measure samples manually 88
Measure samples with SIPS 88
Results 89
Print the results 89
Output to LIMS system 90
Shut down the system 90
6Maintenance 91
Maintenance schedule - general 91

Safety Practices and Hazards
Agilent 55B AA Spectrometer User’s Guide 7
Maintenance schedule – nebulizer/spray chamber 92
Cleaning 93
General 93
Windows 93
Filters 93
Nebulizer 93
Remove the nebulizer block 94
Disassemble the nebulizer 95
Clean a blocked nebulizer 96
Reassemble the nebulizer block 97
Impact bead 101
Spray chamber 108
Disassemble the spray chamber 108
To clean the spray chamber 109
To assemble the spray chamber 109
Assemble the spray chamber and nebulizer block: 111
Attach the drain tube/float/liquid trap to the nebulizer block 111
Installing the spray chamber/nebulizer assembly 113
Burners 116
To install a burner: 116
Clean the burner 118
Gas supplies 122
Lamps 122
To install a hollow cathode lamp: 122
Removing hollow cathode lamps 123

Safety Practices and Hazards
8 Agilent 55B AA Spectrometer User’s Guide
D2lamp 124
Fuses 126
7Spare Parts 129
Sample lists 129
Other 132
Covers/doors 132
Fuses 132
Miscellaneous 133
8Troubleshooting/Errors 135
Common problems 137
Error messages 140
0804 LIMS port error 140
2851 SIPS comms error 140
3800 EEPROM storage error 140
5004 Signals not increasing 141
5005 Slope test failure 141
5006 Calibration fit failure 141
5008 Reslope signal out of range 141
6000 Check HC lamp selection 141
9159 EEPROM checksum zero wl 141
9160 EEPROM checksum mono correction 142
9307 RBC/Mains frequency below 48Hz. 142
9308 RBC/Mains frequency 142
9309 RBC/Mains frequency above 62Hz 142
9310 Optical RBC frequency 143

Safety Practices and Hazards
Agilent 55B AA Spectrometer User’s Guide 9
9311 Instrument fault +12V PSU 143
9312 Instrument fault -12V PSU 143
9313 Instrument fault 5V PSU 143
9316 Wavelength out of range 143
9317 No peak: low HC lamp energy 143
9318 No peak: high HC lamp energy 144
9319 No peak: low BC lamp energy 144
9320 No peak: high BC lamp energy 144
9321 No peak: low HC lamp energy 145
9322 No peak: high HC lamp energy 145
9323 Low emission: no peak 145
9324 High emission: no peak 146
9329 No peak detected 146
9330 No peak detected 146
9337 Instrument fault 310V PSU 147
9339 Mono resetting. Please wait 147
9415 Instrument fault: lamp code 147
9422 Instrument fault: lamp current 147
9514 Instrument signal saturation 148
9517 Low HC lamp energy 148
9518 High HC lamp energy 148
9524 Low BC lamp energy 148
9525 High BC lamp energy 149
9527 Instrument fault: EHT failed 149
9528 Background lamp failed 149
9529 Background lamp interlock 149

Safety Practices and Hazards
10 Agilent 55B AA Spectrometer User’s Guide
9530 Instrument fault: signal diagnostics 149
9531 No lamp current detected 150
9602 Instrument fault: mono datum 150
9911 No burner fitted 150
9912 N2O burner not fitted 150
9914 No gas control unit 150
9915 Flame shield open 150
9916 Fault: gas pressure sensor 151
9917 No oxidant gas pressure 151
9920 Fault: flame detected 151
9921 Flame out detected 151
9922 Flame shutdown: gas type 151
9923 Flame ignition timeout 152
9934 Flame shutdown: host offline 152
9937 Flame pressure relief bung 152
9938 Liquid trap not ready 152
9xxx GPIB fault 152
9xxx Instrument error 152
9xxx SpectrAA error 152