Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Diode Array and Multiple
Wavelength Detector
User Manual

Agilent 1260 Infinity DAD and MWD User Manual
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Manual Part Number
G1315-90013
Edition
06/10
Printed in Germany
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For Research Use Only

Agilent 1260 Infinity DAD and MWD User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1260 Infinity Diode Array and Multiple
Wavelength Detector modules:
•G1315C - 1260 DAD VL+
•G1365C - 1260 MWD
•G1315D - 1260 DAD VL
•G1365D - 1260 MWD VL
1 Introduction
This chapter gives an introduction to the detector, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your system
and the installation of your module.
4 LAN Configuration
This chapter provides information on connecting the detector to the Agilent
ChemStation PC.
5 Using the Detector
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
6 How to optimize the Detector
This chapter provides information on how to optimize the detector.

4 Agilent 1260 Infinity DAD and MWD User Manual
In This Guide…
7 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
9TestFunctions
This chapter describes the detector’s built in test functions.
10 Maintenance
This chapter describes the maintenance of the detector.
11 Parts for Maintenance
This chapter provides information on parts for maintenance.
12 Identifying Cables
This chapter provides information on cables used with the 1200 series of
HPLC modules.
13 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity DAD and MWD User Manual 5
Contents
Contents
1 Introduction 9
Introduction to the Detector 10
Optical System 11
Early Maintenance Feedback (EMF) 14
Instrument Layout 15
Electrical Connections 16
Interfaces 18
Setting the 8-bit Configuration Switch 24
2 Site Requirements and Specifications 33
Site Requirements 34
Physical Specifications 37
Performance Specifications 38
3 Installing the Module 43
Unpacking the Detector 44
Optimizing the Stack Configuration 46
Installing the Detector 50
Flow Connections to the Detector 53
Setting up the LAN access 56
4 LAN Configuration 57
What you have to do first 58
TCP/IP parameter configuration 59
Configuration Switch 60
Initialization mode selection 61
Link configuration selection 65
Automatic Configuration with BootP 66
Storing the settings permanently with Bootp 76
Manual Configuration 77

6 Agilent 1260 Infinity DAD and MWD User Manual
Contents
5 Using the Detector 83
Setting up an Analysis 84
Special Settings of the Detector 100
Special Setups with Multiple DAD-MWDs 114
6 How to optimize the Detector 115
Introduction 116
Optimization Overview 117
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 119
Optimizing Selectivity 129
7 Troubleshooting and Diagnostics 133
Overview of the Module’s Indicators and Test Functions 134
Status Indicators 135
User Interfaces 137
Agilent Lab Advisor Software 138
8 Error Information 139
What Are Error Messages 141
General Error Messages 142
Detector Error Messages 150
9 Test Functions 161
Self-test 162
Filter Test 164
Slit Test 166
Dark-Current Test 167
Intensity Test 170
Holmium Oxide Test 174
Spectral flatness test 177
ASTM Noise Test 178
Cell Test 179
Using the Built-in Test Chromatogram 181
Wavelength Verification and Recalibration 183
Test Chromatogram 184
Diagnosis Information on Agilent ChemStation 186
D/A Converter (DAC) Test 188

Agilent 1260 Infinity DAD and MWD User Manual 7
Contents
10 Maintenance 191
Introduction to Maintenance 192
Cautions and Warnings 193
Overview of Maintenance 195
Cleaning the Module 196
Exchanging a Lamp 197
Exchanging a Flow Cell 200
Maintenance of Standard, Semi-Micro or Micro Flow Cell 203
Maintenance of High Pressure Flow Cell 207
Replacing Capillaries on a Standard Flow Cell 209
Replacing Capillaries on a Semi-Micro and Micro Flow Cell 214
Nano Flow Cell - Replacing or Cleaning 218
Cleaning or Exchanging the Holmium Oxide Filter 223
Correcting Leaks 226
Replacing Leak Handling System Parts 227
Replacing the CompactFlash Card (G1315C/G1365C only) 228
Replacing the Module’s Firmware 229
11 Parts for Maintenance 231
Overview of Maintenance Parts 232
Standard Flow Cell 234
Semi-Micro Flow Cell Parts 236
Micro Flow Cell 238
Prep Flow Cell - SST 240
Prep Flow Cell - Quartz 242
Nano Flow Cells 244
High Pressure Flow Cell 248
Accessory Kits 250
12 Identifying Cables 253
Cable Overview 254
Analog Cables 256
Remote Cables 258
BCD Cables 261
CAN/LAN Cables 263
Agilent 1200 module to PC 264

8 Agilent 1260 Infinity DAD and MWD User Manual
Contents
13 Appendix 265
General Safety Information 266
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 269
Radio Interference 270
Sound Emission 271
UV-Radiation 272
Solvent Information 273
Declaration of Conformity for HOX2 Filter 275
Agilent Technologies on Internet 276

9
Agilent 1260 Infinity DAD and MWD User Manual
Agilent Technologies
1
Introduction
Introduction to the Detector 10
Optical System 11
Early Maintenance Feedback (EMF) 14
Instrument Layout 15
Electrical Connections 16
Serial Number Information (ALL) 17
Rear view of the module 17
Interfaces 18
Interfaces Overview 20
Setting the 8-bit Configuration Switch 24
Communication Settings for RS-232C 28
Special Settings 30
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1260 Infinity DAD and MWD User Manual
1Introduction
Introduction to the Detector
Introduction to the Detector
The detector is designed for highest optical performance, GLP compliance and
easy maintenance. It includes the following features:
•80 Hz data acquisition rate for (ultra-) fast LC applications (requires
internal hard disk, G1315C and G1365C only),
•data recovery (DRC) feature provides data-never-lost insurance (requires
internal hard disk, G1315C and G1365C only),
•RFID tags for all flow cells and UV-lamps provides traceable information
about these assemblies,
•long-life deuterium with RFID tag and tungsten lamps for highest intensity
and lowest detection limit over a wavelength range of 190–950 nm,
•no loss in sensitivity for up to eight wavelengths simultaneous,
•programmable slit from 1–16 nm for complete optimization of sensitivity,
linearity and spectral resolution,
•optional flow-cell cartridges with RFID tag (standard 10 mm 13 µl,
semi-micro 6 mm 5 µl, micro 3 mm 2 µl, 80 nl, 500 nl, 10 mm, high pressure
10 mm 1.7 µl and prep-cells) are available and can be used depending on
the application needs,
•easy front access to lamps and flow cell for fast replacement, and
•built-in holmium oxide filter for fast wavelength accuracy verification,
•built-in temperature control for improved baseline stability,
•additional diagnostic signals for temperature and lamp voltage monitoring,
For specifications, see “Performance Specifications” on page 38.