Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Diode Array and Multiple
Wavelength Detector
User Manual

Agilent 1260 Infinity DAD and MWD User Manual
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© Agilent Technologies, Inc. 2006-2010
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Manual Part Number
G1315-90013
Edition
06/10
Printed in Germany
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For Research Use Only

Agilent 1260 Infinity DAD and MWD User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1260 Infinity Diode Array and Multiple
Wavelength Detector modules:
•G1315C - 1260 DAD VL+
•G1365C - 1260 MWD
•G1315D - 1260 DAD VL
•G1365D - 1260 MWD VL
1 Introduction
This chapter gives an introduction to the detector, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your system
and the installation of your module.
4 LAN Configuration
This chapter provides information on connecting the detector to the Agilent
ChemStation PC.
5 Using the Detector
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
6 How to optimize the Detector
This chapter provides information on how to optimize the detector.

4 Agilent 1260 Infinity DAD and MWD User Manual
In This Guide…
7 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
9TestFunctions
This chapter describes the detector’s built in test functions.
10 Maintenance
This chapter describes the maintenance of the detector.
11 Parts for Maintenance
This chapter provides information on parts for maintenance.
12 Identifying Cables
This chapter provides information on cables used with the 1200 series of
HPLC modules.
13 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity DAD and MWD User Manual 5
Contents
Contents
1 Introduction 9
Introduction to the Detector 10
Optical System 11
Early Maintenance Feedback (EMF) 14
Instrument Layout 15
Electrical Connections 16
Interfaces 18
Setting the 8-bit Configuration Switch 24
2 Site Requirements and Specifications 33
Site Requirements 34
Physical Specifications 37
Performance Specifications 38
3 Installing the Module 43
Unpacking the Detector 44
Optimizing the Stack Configuration 46
Installing the Detector 50
Flow Connections to the Detector 53
Setting up the LAN access 56
4 LAN Configuration 57
What you have to do first 58
TCP/IP parameter configuration 59
Configuration Switch 60
Initialization mode selection 61
Link configuration selection 65
Automatic Configuration with BootP 66
Storing the settings permanently with Bootp 76
Manual Configuration 77

6 Agilent 1260 Infinity DAD and MWD User Manual
Contents
5 Using the Detector 83
Setting up an Analysis 84
Special Settings of the Detector 100
Special Setups with Multiple DAD-MWDs 114
6 How to optimize the Detector 115
Introduction 116
Optimization Overview 117
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 119
Optimizing Selectivity 129
7 Troubleshooting and Diagnostics 133
Overview of the Module’s Indicators and Test Functions 134
Status Indicators 135
User Interfaces 137
Agilent Lab Advisor Software 138
8 Error Information 139
What Are Error Messages 141
General Error Messages 142
Detector Error Messages 150
9 Test Functions 161
Self-test 162
Filter Test 164
Slit Test 166
Dark-Current Test 167
Intensity Test 170
Holmium Oxide Test 174
Spectral flatness test 177
ASTM Noise Test 178
Cell Test 179
Using the Built-in Test Chromatogram 181
Wavelength Verification and Recalibration 183
Test Chromatogram 184
Diagnosis Information on Agilent ChemStation 186
D/A Converter (DAC) Test 188

Agilent 1260 Infinity DAD and MWD User Manual 7
Contents
10 Maintenance 191
Introduction to Maintenance 192
Cautions and Warnings 193
Overview of Maintenance 195
Cleaning the Module 196
Exchanging a Lamp 197
Exchanging a Flow Cell 200
Maintenance of Standard, Semi-Micro or Micro Flow Cell 203
Maintenance of High Pressure Flow Cell 207
Replacing Capillaries on a Standard Flow Cell 209
Replacing Capillaries on a Semi-Micro and Micro Flow Cell 214
Nano Flow Cell - Replacing or Cleaning 218
Cleaning or Exchanging the Holmium Oxide Filter 223
Correcting Leaks 226
Replacing Leak Handling System Parts 227
Replacing the CompactFlash Card (G1315C/G1365C only) 228
Replacing the Module’s Firmware 229
11 Parts for Maintenance 231
Overview of Maintenance Parts 232
Standard Flow Cell 234
Semi-Micro Flow Cell Parts 236
Micro Flow Cell 238
Prep Flow Cell - SST 240
Prep Flow Cell - Quartz 242
Nano Flow Cells 244
High Pressure Flow Cell 248
Accessory Kits 250
12 Identifying Cables 253
Cable Overview 254
Analog Cables 256
Remote Cables 258
BCD Cables 261
CAN/LAN Cables 263
Agilent 1200 module to PC 264

8 Agilent 1260 Infinity DAD and MWD User Manual
Contents
13 Appendix 265
General Safety Information 266
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 269
Radio Interference 270
Sound Emission 271
UV-Radiation 272
Solvent Information 273
Declaration of Conformity for HOX2 Filter 275
Agilent Technologies on Internet 276

9
Agilent 1260 Infinity DAD and MWD User Manual
Agilent Technologies
1
Introduction
Introduction to the Detector 10
Optical System 11
Early Maintenance Feedback (EMF) 14
Instrument Layout 15
Electrical Connections 16
Serial Number Information (ALL) 17
Rear view of the module 17
Interfaces 18
Interfaces Overview 20
Setting the 8-bit Configuration Switch 24
Communication Settings for RS-232C 28
Special Settings 30
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1260 Infinity DAD and MWD User Manual
1Introduction
Introduction to the Detector
Introduction to the Detector
The detector is designed for highest optical performance, GLP compliance and
easy maintenance. It includes the following features:
•80 Hz data acquisition rate for (ultra-) fast LC applications (requires
internal hard disk, G1315C and G1365C only),
•data recovery (DRC) feature provides data-never-lost insurance (requires
internal hard disk, G1315C and G1365C only),
•RFID tags for all flow cells and UV-lamps provides traceable information
about these assemblies,
•long-life deuterium with RFID tag and tungsten lamps for highest intensity
and lowest detection limit over a wavelength range of 190–950 nm,
•no loss in sensitivity for up to eight wavelengths simultaneous,
•programmable slit from 1–16 nm for complete optimization of sensitivity,
linearity and spectral resolution,
•optional flow-cell cartridges with RFID tag (standard 10 mm 13 µl,
semi-micro 6 mm 5 µl, micro 3 mm 2 µl, 80 nl, 500 nl, 10 mm, high pressure
10 mm 1.7 µl and prep-cells) are available and can be used depending on
the application needs,
•easy front access to lamps and flow cell for fast replacement, and
•built-in holmium oxide filter for fast wavelength accuracy verification,
•built-in temperature control for improved baseline stability,
•additional diagnostic signals for temperature and lamp voltage monitoring,
For specifications, see “Performance Specifications” on page 38.

Agilent 1260 Infinity DAD and MWD User Manual 11
Introduction 1
Optical System
Optical System
The optical system of the detector is shown in Figure below. Its illumination
source is a combination of a deuterium-arc-discharge lamp for the ultraviolet
(UV) wavelength range and a tungsten lamp for the visible (VIS) and
short-wave near-infrared (SWNIR) wavelength range. The image of the
filament of the tungsten lamp is focused on the discharge aperture of the
deuterium lamp by means of a special rear-access lamp design which allows
both light sources to be optically combined and share a common axis to the
source lens. The achromat (source lens) forms a single, focused beam of light
through the flow cell. Each cell room and lamp are separated by a quartz
window which can be cleaned or replaced. In the spectrograph, light is being
dispersed onto the diode array by a holographic grating. This allows
simultaneous access to all wavelength information.
Figure 1 Optical System of the Detector
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12 Agilent 1260 Infinity DAD and MWD User Manual
1Introduction
Optical System
Lamps The light source for the UV-wavelength range is a deuterium lamp with a
shine-through aperture. As a result of plasma discharge in low-pressure
deuterium gas, the lamp emits light over the 190 nm to approximately 800 nm
wavelength range. The light source for the visible and SWNIR wavelength
range is a low noise tungsten lamp. This lamp emits light over the wavelength
range 470 – 950 nm.
Achromat
(Source Lens)
The achromat receives the light from both lamps and focuses it so that the
beam passes through the flow cell.
Holmium Oxide
Filter
The holmium oxide filter is electromechanically actuated. During the holmium
filter test it moves into the light path.
Cell Support
Window
The cell support window assembly separates the holmium filter area from the
flow cell area.
Flow Cell
Compartment
The optical unit has a flow cell compartment for easy access to flow cells. A
variety of optional flow cells can be inserted using the same quick, simple
mounting system. The flow cell can be removed to check the optical and
electronic performance of the detector without having influences from the
flow cell.
Spectrograph The spectrograph material is ceramic to reduce thermal effects to a minimum.
The spectrograph consists of the spectrograph lens, the variable entrance slit,
the grating and the photodiode array with front-end electronics. The
spectrograph lens refocuses the light beam after it has passed through the flow
cell. The sampling interval of the diode array is < 1 nm over the wavelength
range 190 – 950 nm. Depending on the wavelength this varies from 1.0 to 1.25
diodes per nanometer (for example a diode every 0.8 to 1 nm).
For a small wavelength range, the small non-linearity could be neglected. With
the wavelength range from 190 – 950 nm a new approach is required to
achieve wavelength accuracy over the full range. Each spectrograph is
calibrated individually. The calibration data is stored in the spectrograph on
an EEPROM. Based on these data, the built-in processors calculate absorbance
data with linear intervals (1.0, 2.0, …) between data points. This results in an
excellent wavelength accuracy and instrument-to-instrument reproducibility.
Variable Entrance
Slit System
The micro-slit system makes use of the mechanical properties of silicon
combined with the precise structuring capabilities of bulk micro-machining. It
combines the required optical functions — slit and shutter — in a simple and
compact component. The slit width is directly controlled by the
micro-processor of the instrument and can be set as method parameter.

Agilent 1260 Infinity DAD and MWD User Manual 13
Introduction 1
Optical System
Grating The combination of dispersion and spectral imaging is accomplished by using
a concave holographic grating. The grating separates the light beam into all its
component wavelengths and reflects the light onto the photodiode array.
Diode Array The diode array is a series of 1024 individual photodiodes and control circuits
located on a ceramic carrier. With a wavelength range from 190 – 950 nm the
sampling interval is < 1 nm.

14 Agilent 1260 Infinity DAD and MWD User Manual
1Introduction
Early Maintenance Feedback (EMF)
Early Maintenance Feedback (EMF)
Maintenance requires the exchange of components which are subject to wear
or stress. Ideally, the frequency at which components are exchanged should be
based on the intensity of usage of the module and the analytical conditions,
and not on a predefined time interval. The early maintenance feedback (EMF)
feature monitors the usage of specific components in the instrument, and
provides feedback when the user-selectable limits have been exceeded. The
visual feedback in the user interface provides an indication that maintenance
procedures should be scheduled.
EMF Counters
EMF counters increment with use and can be assigned a maximum limit which
provides visual feedback in the user interface when the limit is exceeded.
Some counters can be reset to zero after the required maintenance procedure.
Using the EMF Counters
The user-settable EMF limits for the EMF Counters enable the early maintenance
feedback to be adapted to specific user requirements. The useful maintenance
cycle is dependent on the requirements for use. Therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.
Setting the EMF Limits
The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially the default EMF limits should be set. When instrument
performance indicates maintenance is necessary, take note of the values
displayed by the EMF counters. Enter these values (or values slightly less than
the displayed values) as EMF limits, and then reset the EMF counters to zero.
The next time the EMF counters exceed the new EMF limits, the EMF flag will be
displayed, providing a reminder that maintenance needs to be scheduled.

Agilent 1260 Infinity DAD and MWD User Manual 15
Introduction 1
Instrument Layout
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers of foam plastic spacers in which the mechanical
and electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
•virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
•the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
•the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
•the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.

16 Agilent 1260 Infinity DAD and MWD User Manual
1Introduction
Electrical Connections
Electrical Connections
•The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer and
synchronization.
•Two independent analog outputs provide signals for integrators or data
handling.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
•With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
•The power input socket accepts a line voltage of 100 – 240 VAC ± 10 % with a
line frequency of 50 or 60 Hz. Maximum power consumption varies by
module. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.

Agilent 1260 Infinity DAD and MWD User Manual 17
Introduction 1
Electrical Connections
Serial Number Information (ALL)
The serial number information on the instrument labels provide the following
information:
Rear view of the module
Figure 2 Rear View of Detector
CCXZZ00000 Format
CC Country of manufacturing (DE Germany)
X Alphabetic character A-Z (used by manufacturing)
ZZ Alpha-numeric code 0-9, A-Z, where each combination
unambiguously denotes a module (there can be more than one
code for the same module)
00000 Serial number

18 Agilent 1260 Infinity DAD and MWD User Manual
1Introduction
Interfaces
Interfaces
The Agilent 1200 Infinity Series modules provide the following interfaces:
Tab le 1 Agilent 1200 Infinity Series Interfaces
Module CAN LAN/BCD
(optional)
LAN
(on-board)
RS-232 Analog APG
Remote
Special
Pumps
G1310B Iso Pump
G1311B Quat Pump
G1311C Quat Pump VL
G1312B Bin Pump
G1312C Bin Pump VL
1376A Cap Pump
G2226A Nano Pump
2 Yes No Yes 1 Yes
G4220A/B Bin Pump 2 No Yes Yes No Yes
G1361A Prep Pump 2 Yes No Yes No Yes CAN-DC- OUT for CAN
slaves
Samplers
G1329B ALS
G2260A Prep ALS
2 Yes No Yes No Yes THERMOSTAT for
G1330B
G1364B FC-PS
G1364C FC-AS
G1364D FC-μS
G1367E HiP ALS
G1377A HiP micro ALS
G2258A DL ALS
2 Yes No Yes No Yes THERMOSTAT for
G1330B
CAN-DC- OUT for CAN
slaves
G4226A ALS 2 Yes No Yes No Yes
Detectors
G1314B VWD VL
G1314C VWD VL+
2 Yes No Yes 1 Yes
G1314E/F VWD 2 No Yes Yes 1 Yes

Agilent 1260 Infinity DAD and MWD User Manual 19
Introduction 1
Interfaces
•CAN connectors as interface to other modules
•LAN connector as interface to the control software
•RS-232C as interface to a computer
•REMOTE connector as interface to other Agilent products
•Analog output connector(s) for signal output
G4212A/B DAD 2 No Yes Yes 1 Yes
G1315C DAD VL+
G1365C MWD
G1315D DAD VL
G1365D MWD VL
2 No Yes Yes 2 Yes
G1321B FLD
G1362A RID
2 Yes No Yes 1 Yes
G4280A ELSD No No No Yes Yes Yes EXT Contact
AUTOZERO
Others
G1316A/C TCC 2 No No Yes No Yes
G1322A DEG No No No No No Yes AUX
G1379B DEG No No No Yes No No AUX
G4227A Flex Cube 2 No No No No No
G4240A CHIP CUBE 2 Yes No Yes No Yes CAN-DC- OUT for CAN
slaves
THERMOSTAT for
G1330A/B (NOT USED)
Tab le 1 Agilent 1200 Infinity Series Interfaces
Module CAN LAN/BCD
(optional)
LAN
(on-board)
RS-232 Analog APG
Remote
Special
NOTE The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via
LAN. The inter-module communication is done via CAN.

20 Agilent 1260 Infinity DAD and MWD User Manual
1Introduction
Interfaces
Interfaces Overview
CAN
The CAN is inter-module communication interface. It is a 2-wire serial bus
system supporting high speed data communication and real-time requirement.
LAN
The modules have either an interface slot for an LAN card (e.g. Agilent
G1369A/B LAN Interface) or they have an on-board LAN interface (e.g.
detectors G1315C/D DAD and G1365C/D MWD). This interface allows the
control of the module/system via a connected PC with the appropriate control
software.
RS-232C (Serial)
The RS-232C connector is used to control the module from a computer
through RS-232C connection, using the appropriate software. This connector
can be configured with the configuration switch module at the rear of the
module. Refer to Communication Settings for RS-232C.
The RS-232C is designed as DCE (data communication equipment) with a
9-pin male SUB-D type connector. The pins are defined as:
NOTE If an Agilent detector (DAD/MWD/FLD/VWD/RID) is in the system, the LAN should be
connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If no Agilent
detector is part of the system, the LAN interface should be installed in the pump or
autosampler.
NOTE There is no configuration possible on main boards with on-board LAN. These are
pre-configured for
•19200 baud,
•8 data bit with no parity and
•one start bit and one stop bit are always used (not selectable).