Agilent Technologies 1260 Infinity II User manual

Agilent 1260 Infinity II
Multi-Angle Light Scattering Detector
User Manual

Notices
Manual Part Number
Document No: D0029607 Rev. B
Edition 07/2023
Copyright
© Agilent Technologies, Inc. 2023
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Safety Notices
A CAUTION notice denotes a hazard. It
calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a hazard.
It calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indicated
conditions are fully understood and
met.
WARNING
CAUTION

Multi-Angle Light Scattering Detector User Manual 3
In this Guide….
This manual covers the Agilent 1260 Infinity II Multi-Angle Light Scattering Detector
(G7885A).
Please read this manual thoroughly before using the instrument!
Thank you for purchasing this detector from Agilent Technologies. You have
acquired a state-of-the-art instrument which enables you to perform unique
experiments and the characterization of the most complex particle, polymer and
protein samples.
This manual has been written for laboratory technicians who are responsible for
operation and maintenance of laboratory equipment. It is assumed that the user of
this manual is familiar with standard laboratory terminology. To ensure optimum
operation conditions, please read the manual completely and carefully before using
the detector. Use this product only in accordance with the guidelines and
instructions listed.
This manual describes in detail:
Characteristics
Operation and Maintenance
Important:
Do not use this instrument before completely understanding the full content of
this manual.
In the event that the instrument is loaned or transferred to a new owner please
ensure this manual is passed on with the device.
If this manual or any warning labels attached to the device become lost, get
damaged or are unreadable, promptly obtain replacement documents/ labels
from your nearest Agilent representative / distributor.
To ensure safe operation at any time, please read the safety instructions before
using the instrument.
In case of any uncertainty how to operate this detector, consult your Agilent
representative before starting to use this product.
NOTE Agilent offers basic, advanced and repeated trainings to support the user.

Multi-Angle Light Scattering Detector User Manual 4
Contents
1Introduction 6
After-Sales Service 7
Safety Instructions 8
Safety Symbols 16
Device Characteristics 17
2Operation 31
General Handling 32
Commissioning 35
User Interface 37
Changing the Solvent 39
Storage 40
Leak Detection 41
Vapor Detection 44
3Maintenance 46
Preventive Maintenance 47
Routine Maintenance 48
Detector Cleaning 49
4Troubleshooting 53
System Problems 54

Multi-Angle Light Scattering Detector User Manual 5
5Identified Cables 56
Cable and Connector Specifications 57
6Appendix 58
Waste Electrical and Electronic Equipment Directive 59
Agilent Technologies on Internet 60
Miscibility Chart of Solvents 61

Introduction
Multi-Angle Light Scattering Detector User Manual 6
1Introduction
After-Sales Service 7
Safety Instructions 8
Site Requirements 9
Power Considerations 10
Solvent Information 11
Using the Instrument 12
Maintaining the Instrument 13
Cleaning the Instrument 14
Handling Leak and Waste 14
Laser Warning 14
Static Electricity Warning 15
Safety Symbols 16
Device Characteristics 17
Delivery Content 17
General Purpose 17
Working Principle 18
Safety Functions 22
Physical Specifications 23
Performance Specifications 24
Detector Setup 26
Rear Side 28
Detection Angles 30
This chapter provides an overview of the principles and theory of Light Scattering
detection. Furthermore, the most important functions as well as specifications of
the detector are discussed.

Introduction
Multi-Angle Light Scattering Detector User Manual 7
After-Sales Service
If any problem occurs with this instrument, inspect it immediately and take
appropriate corrective action as described in chapter “Troubleshooting”on page 53.
If the problem persists or symptoms occur that are not described in this document,
disconnect the mains voltage and contact your Agilent representative.
Maintenance
Each component and the entire system should be checked periodically to ensure
that they function normally, otherwise the analysis data may not be precise and
reliable anymore. Typically, a yearly service is recommended.
Agilent offers special maintenance and service contracts to ensure maximum
lifetime and proper operation of the instrument during the entire life cycle. Before
shipment from the factory, this device was thoroughly inspected and tested.
The result of the inspection is documented in the attached protocol. Referring to
this protocol, please check the results of the hardware validation on a regular basis.

Introduction
Multi-Angle Light Scattering Detector User Manual 8
Safety Instructions
The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed a comparable vocational training.
The following knowledge is required:
Fundamental understanding of liquid chromatography.
Familiarity with substances that are used for laboratory experiments.
Awareness regarding the health risks of chemicals.
Participation during a training by Agilent or an authorized person.
If you do not belong to this or a comparable professional group, you may not
perform the work described in this user manual under any circumstances. In this
case, please contact your superior.
The manufacturer does not warrant for any damage or injury resulting from
incorrect operation and maintenance, non-observance of the instructions in this
manual. Prior to delivery, this instrument has been tested for safe usage.
To ensure safe operation of the instrument, please read these safety
instructions carefully before use and follow them always while operating the
instrument.
Observe all of the Warning and Caution sections listed below. They are
extremely important for safety and to maintain a proper working device.
In this manual, Warning / Caution messages are always indicated using the
following conventions:
WARNING
A warning alerts you to situations that could cause physical injury or death.
Do not proceed beyond a warning until you have fully understood and met
the indicated conditions.
CAUTION A caution alerts you to situations that could cause loss of data, or damage of
equipment.
Do not proceed beyond a caution until you have fully understood and met the
indicated conditions.

Introduction
Multi-Angle Light Scattering Detector User Manual 9
Site Requirements
WARNING
•
Pay attention to the weight of the detectors. The detector weighs 16.5 kg
(36.4 lbs). Use proper lifting techniques to avoid potential injuries. Ensure
that you can handle the weight of the load.
•
Consider the entire weight of the combined system including all the
components. The lab table on which this detector is installed should be
sufficiently strong to support the total weight of the whole LC system. It
should be level, stable and have a depth of at least 600 mm. Otherwise, the
instrument could tip over or fall off the table.
•
Take measures to prevent the instrument from falling in the event of an
earthquake or other disaster. Strong vibrations could cause the detector to
fall over, resulting in injury and damage.
•
All other equipment connected to this instrument must be approved to an
appropriate safety standard and have reinforced insulation from the mains.
CAUTION
•
Avoid installation sites that are exposed to corrosive gases, strong vibrations, or
excessive dust. Otherwise, the performance and operability of the detector could
be impaired, and its lifetime may be shortened.
•Never place any liquid on top of the detector.
Bench Space
The module dimensions and weight (see Table “Physical Specifications”on page
23) allow you to place the module on almost any desk or laboratory bench. It needs
an additional 2.5 cm (1.0 inch) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete LC system, make sure that the bench is
designed to bear the weight of all modules.
NOTE The instrument is heavy and thus it is advisable to locate the system on a sturdy
bench.
NOTE Allow additional bench space for a PC if the instrument is to be computer-
controlled.

Introduction
Multi-Angle Light Scattering Detector User Manual 10
Power Considerations
WARNING
•
Ground the instrument.
To prevent electric shock in the event of an accident or electrical discharge,
the detector must be connected to protective earth. This is also important
to ensure stable operation and good data signals.
Ensure that all connected devices are grounded via the mains connector as
well.
•
Do not place heavy objects onto the power cord and keep any hot objects
away. This can damage the cord, resulting in fire, electrical shock, or
malfunction. If the cord is damaged, immediately unplug the mains power
cord and contact your Agilent representative.
•
Never modify the supplied power cord. Do not bend it excessively or pull on
it. Failure to do so could damage the cord, resulting in fire, electrical shock,
or malfunction.
•
Never replace the detachable power cord by an inadequately rated cord.
This can result in fire, electrical shock, or malfunction.
•
The power supply voltages and power consumption of this instrument are
listed in table “Physical Specifications”on page 23. Connect the detector
only to a power supply of the voltage indicated. Otherwise, fire or electric
shock may occur. Ensure that the power supply voltage is stable and has
sufficiently constant current capacity to operate all the components of the
system. If not, the detector will not operate at its rated performance levels.
•
If the power cord or its insulation gets damaged, unplug the cord and
contact your Agilent representative for a replacement cable immediately.
•
Only use mains connection cables admitted for use in your country. We
strongly recommend to only use cables supplied by Agilent.
•
In case of danger, immediately unplug the mains power cord either at the
wall power outlet, the power strip or on the rear of the devices.