Agilent Technologies InfinityLab LC Series User manual

Agilent InfinityLab LC Series
Variable Wavelength Detectors
User Manual
Agilent InfinityLab LC Series VWD User Manual

Agilent InfinityLab LC Series VWD User Manual
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Document No: SD-29000240
Part No: G7114-90001 Rev. C
EDITION 10/2019
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Agilent InfinityLab LC Series VWD User Manual 3
In This Book
This manual covers:
•the Agilent 1290 Infinity II Variable Wavelength Detector (G7114B) and
•the Agilent 1260 Infinity II Variable Wavelength Detector (G7114A).
Find information on other Agilent Variable Wavelength Detectors in separate
manuals.
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements, physical and
performance specifications.
3 Using the Module
This chapter explains the essential operational parameters of the module.
4 Preparing the Module
This chapter provides information on how to set up the detector for an analysis
and explains the basic settings.
5 Optimizing the Detector
This chapter provides information on how to optimize the detector.
6Troubleshootingand Diagnostics
Overview about the troubleshooting and diagnostic features.

Agilent InfinityLab LC Series VWD User Manual 4
7ErrorInformation
This chapter describes the meaning of detector error messages, and provides
information on probable causes and suggested actions how to recover from error
conditions.
8 Test Functions
This chapter describes the detector’s built in test functions.
9 Maintenance
This chapter provides general information on maintenance of the detector.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200 Infinity
Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent InfinityLab LC Series VWD User Manual 5
Contents
1 Introduction to the Variable Wavelength Detector 9
Introduction to the Detector 10
Product Description 11
Optical System Overview 13
Dual-Wavelength Mode 18
Leak and Waste Handling 19
Operating Principle 24
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 30
3 Using the Module 35
Magnets 36
Turn on/off 37
Status Indicators 39
Instrument Configuration 40
Set up the Detector with Agilent Open Lab ChemStation 42
The Detector User Interface 43
Detector Control Settings 45
Method Parameter Settings 46
Scanning with the VWD 50
Agilent Local Control Modules 52
4 Preparing the Module 54
Leak and Waste Handling 55
Setting up an Analysis 57
Solvent Information 64

Agilent InfinityLab LC Series VWD User Manual 6
5 Optimizing the Detector 69
Introduction 70
Match the Flow Cell to the Column 71
Set the Detector Parameters 74
Warm up of the Detector 75
6 Troubleshooting and Diagnostics 77
Available Tests versus Interfaces 78
Agilent Lab Advisor Software 79
7ErrorInformation80
What Are Error Messages 81
General Error Messages 82
Detector Error Messages 89
8 Test Functions 99
Introduction 100
Intensity Test 105
Cell Test 108
Wavelength Verification-Calibration 110
ASTM Drift and Noise Test 112
Quick Noise Test 113
Dark Current Test 115
Holmium Oxide Test 117
D/A Converter (DAC) Test 120
Other Lab Advisor Functions 123

Agilent InfinityLab LC Series VWD User Manual 7
9 Maintenance 124
Introduction to Maintenance 125
Warnings and Cautions 126
Overview of Maintenance 128
Cleaning the Module 129
Remove and Install the Doors 130
Replace the Deuterium Lamp 132
Replace the Flow Cell / Cuvette Holder 135
Repair the Flow Cells 137
Using the Cuvette Holder 141
Correcting Leaks 143
Replace Leak Handling System Parts 145
Replace the Module Firmware 146
10 Parts and Materials for Maintenance 147
Overview of Maintenance Parts 148
Standard Flow Cell 10 mm / 14 µl 150
Micro Flow Cell 3 mm / 2 µl 152
Semi-micro Flow Cell (Parts) 154
High Pressure Flow Cell (Parts) 156
Cuvette Holder (Parts) 157
Accessory Kit 158
11 Identifying Cables 159
Cable Overview 160
Analog Cables 162
Remote Cables 164
CAN Cable 168
RS-232 Cables 169
USB Cables 170

Agilent InfinityLab LC Series VWD User Manual 8
12 Hardware Information 171
Firmware Description 172
Electrical Connections 175
Interfaces 178
Setting the 6-bit Configuration Switch 186
Instrument Layout 190
Early Maintenance Feedback (EMF) 191
13 LAN Configuration 193
What You Have to Do First 194
TCP/IP parameter configuration 195
Configuration Switch 196
Initialization Mode Selection 197
Dynamic Host Configuration Protocol (DHCP) 199
Manual Configuration 202
14 Appendix 206
General Safety Information 207
Waste Electrical and Electronic Equipment (WEEE) Directive 216
Radio Interference 217
Sound Emission 218
Solvent Information 219
Declaration of Conformity for HOX2 Filter 220
Agilent Technologies on Internet 221

Agilent InfinityLab LC Series VWD User Manual 9
1 Introduction to the Variable Wavelength
Detector
Introduction to the Detector 10
Product Description 11
Product Description G7114A 11
Product Description G7114B 12
Optical System Overview 13
Flow Cell 14
Lamp 15
Source Lens Assembly 15
Entrance Slit Assembly 15
Filter Assembly 16
Mirror Assemblies M1 and M2 17
Grating Assembly 17
Beam Splitter Assembly 17
Photo Diodes Assemblies 17
Photo Diode ADC (analog-to-digital converter) 17
Dual-Wavelength Mode 18
Leak and Waste Handling 19
Leak Sensor 23
Waste Concept 23
Operating Principle 24
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

Agilent InfinityLab LC Series VWD User Manual 10
1 Introduction to the Variable Wavelength Detector
Introduction to the Detector
Introduction to the Detector
The Agilent variable wavelength detectors described in this manual are designed
for highest optical performance, GLP compliance and easy maintenance with:
•higher data rate up to 120 Hz (G7114A) or 240 Hz for ultra-fast-HPLC
(G7114B),
•deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
•optional flow-cell cartridges (standard 10 mm, 14 µL; high pressure 10 mm,
14µL;micro3mm,2µL;semi-micro6mm,5µL)andPrepCellsareavailable
and can be used depending on the application needs (other types may be
introduced later),
•Dual wavelength mode, see “Dual-Wavelength Mode” on page 18.
•easy front access to lamp and flow cell for fast replacement,
•electronic identification of flow cell and lamp with RFID (Radio Frequency
Identification) tag for unambiguous identification,
•lamp information: part number, serial number, production date, ignitions,
burn time
•cell information: part number, serial number, production date, nominal
path length, volume, maximum pressure
•built-in electronic temperature control (ETC) for improved baseline stability,
and
•built-in holmium oxide filter for fast wavelength accuracy verification.

Agilent InfinityLab LC Series VWD User Manual 11
1 Introduction to the Variable Wavelength Detector
Product Description
Product Description
Product Description G7114A
The Agilent 1260 Infinity II Variable Wavelength Detector (VWD) is the most
sensitive and fastest detector in its class.
Time-programmable wavelength switching provides sensitivity and selectivity for
your applications.
More sample information can be acquired in the dual wavelength mode.
Low detector noise (<±1.5 µAU) and baseline drift (< 1 x 10
-4
AU/h) facilitates
precise quantification of trace levels components.
High productivity can be achieved with fast analysis at up to 120 Hz data rates.
Figure 1 Overview of the detector
Status indicator
Flow cell
Lamp housing window
and air inlet
Power switch
Leak drain

Agilent InfinityLab LC Series VWD User Manual 12
1 Introduction to the Variable Wavelength Detector
Product Description
Product Description G7114B
The Agilent 1290 Infinity II Variable Wavelength Detector (VWD) is the most
sensitive and fastest detector in its class.
Time-programmable wavelength switching provides sensitivity and selectivity for
your applications.
More sample information can be acquired in the dual wavelength mode.
Low detector noise (<±1.5 µAU) and baseline drift (<1·10
-4
AU/h) facilitates
precise quantification of trace levels components.
High productivity can be achieved with fast analysis at up to 240 Hz data rates.
Figure 2 Overview of the detector
Status indicator
Flow cell
Lamp housing window
and air inlet
Power switch
Leak drain

Agilent InfinityLab LC Series VWD User Manual 13
1 Introduction to the Variable Wavelength Detector
Optical System Overview
Optical System Overview
The optical system of the detector is shown in the figure below. Its radiation
source is a deuterium-arc discharge lamp for the ultraviolet (UV) wavelength
range from 190 to 600 nm. The light beam from the deuterium lamp passes
through a lens, a filter assembly, an entrance slit, a spherical mirror (M1), a
grating, a second spherical mirror (M2), a beam splitter, and finally through a flow
cell to the sample diode. The beam through the flow cell is absorbed depending
on the solutions in the cell, in which UV absorption takes place, and the intensity
is converted to an electrical signal by means of the sample photodiode. Part of
the light is directed to the reference photodiode by the beam splitter to obtain a
reference signal for compensation of intensity fluctuation of the light source. A
slit in front of the reference photodiode cuts out light of the sample bandwidth.
Wavelength selection is made by rotating the grating, which is driven directly by a
stepper motor. This configuration allows fast change of the wavelength. The
cutoff filter is moved into the lightpath above 370 nm to reduce higher order light.
Figure 3 Optical Path of the Variable Wavelength Detector
Deuterium lamp
Filter assembly
Lens
Entrance slit
Mirror M1
Mirror M2
Grating
Reference diode
Sample diode
Flow cell
Beam splitter

Agilent InfinityLab LC Series VWD User Manual 14
1 Introduction to the Variable Wavelength Detector
Optical System Overview
Flow Cell
A variety of flow-cell cartridges can be inserted using the same quick and simple
mounting system.
The flow cells have an integrated RFID tag that contains the flow cell specific
information (e.g. part number, cell volume, path length, ...). A RFID tag reader
reads out this information and transfers it to the user interface.
Figure 4 Flow Cell with RFID tag
RFID tag
Table 1 Flow Cell Data
STD Semi-micro Micro High Pressure
Maximum pressure 40 (4) 40 (4) 120 (12) 400 (40) bar (MPa)
Path length 10 (conical) 6 (conical) 3 (conical) 10 (conical) mm
Volume 14 5 2 14 µL
Inlet i.d. 0.25 0.17 0.12 0.25 mm
Inlet length 750 250 310 750 mm
Outlet i.d. 0.30 0.17 0.17 0.17 mm
Outlet length 120 120 120 120 mm
Total volume 60.77 14.49 14.00 60.77 µL
Materials in contact
with solvent
SST, quartz, PTFE,
PEEK
SST, quartz, PTFE SST, quartz, PTFE SST, quartz, Kapton

Agilent InfinityLab LC Series VWD User Manual 15
1 Introduction to the Variable Wavelength Detector
Optical System Overview
Lamp
The light source for the UV wavelength range is a deuterium lamp. As a result of
plasma discharge in a low pressure deuterium gas, the lamp emits light over the
190 – 600 nm wavelength range.
The lamp has an integrated RFID tag that contains the lamp specific information
(e.g. part number, burn time, ...). An RFID tag reader reads out this information
and transfers it to the user interface.
Source Lens Assembly
The source lens receives the light from the deuterium lamp and focuses it onto
the entrance slit.
Entrance Slit Assembly
The entrance slit assembly has an exchangeable slit. The standard one has a
1-mm slit. For replacement and calibration purposes to optimize the alignment, a
slit with a hole is needed.

Agilent InfinityLab LC Series VWD User Manual 16
1 Introduction to the Variable Wavelength Detector
Optical System Overview
Filter Assembly
The filter assembly is electromechanically actuated. During wavelength
calibrations it moves into the light path.
Figure 5 Filter Assemby
The filter assembly has two filters installed and is processor-controlled.
A photo sensor determines the correct position.
OPEN nothing in light path at
λ <370nm
CUTOFF cut off filter in light path at
λ > 370 nm
HOLMIUM holmium oxide filter for wavelength check
SHUTTER for measurement of dark current of photo diodes
Cutoff Filter
Shutter
Cutoff Filter plus Holmium Oxide Filter

Agilent InfinityLab LC Series VWD User Manual 17
1 Introduction to the Variable Wavelength Detector
Optical System Overview
Mirror Assemblies M1 and M2
The instrument contains two spherical mirrors (M1 and M2). The beam
adjustable is vertically and horizontally. Both mirrors are identical.
Grating Assembly
The grating separates the light beam into all its component wavelengths and
reflects the light onto mirror #2.
The stepper motor reference position is determined by a plate fitted onto the
motor shaft, interrupting the beam of a photo sensor. The wavelength calibration
of the grating is done at the zero order light position and at 656 nm, which is the
emission line of the deuterium lamp.
Beam Splitter Assembly
The beam splitter splits the light beam. One part goes directly to the sample
diode. The other part of the light beam goes to the reference diode.
Photo Diodes Assemblies
Two photo diode assemblies are installed in the optical unit. The sample diode
assembly is located on the left side of the optical unit. The reference diode
assembly is located in the front of the optical unit.
Photo Diode ADC (analog-to-digital converter)
The photo diode current is directly converted to digital data direct photo current
digitalization. The data is transferred to the detector main board . The photo
diode ADC boards are located close to the photo diodes.

Agilent InfinityLab LC Series VWD User Manual 18
1 Introduction to the Variable Wavelength Detector
Dual-Wavelength Mode
Dual-Wavelength Mode
The detector provides a Dual-Wavelength mode that offers additional operation
functions.
Features:
•200 ms acquisition time per data point
•5 Hz data rate distributed to two channels
•2.5 Hz data rate for each channel,
•delta wavelength max. 150 nm,
•scans during Dual-Wavelength mode are possible,
•the second order filter is disabled when one wavelength is < 370 nm.
Timetable:
•Wavelength settings are timetable programmable (depends if enough time for
implementation is available),
•switching from Single-Wavelength mode to Dual-Wavelength mode is NOT
timetable programmable,
•filter settings are not timetable programmable.

Agilent InfinityLab LC Series VWD User Manual 19
1 Introduction to the Variable Wavelength Detector
Leak and Waste Handling
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should not
exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent
Cabinets (a printed copy of the guideline has been shipped with the solvent
cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity and
Infinity II modules can be stacked on top of each other. Waste tubes are guided
through a channel on the right hand side of the instrument, keeping the front
access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler or Sample Thermostat (condensate)
•from the pump's Seal Wash Sensor (if applicable)
•from the pump's Purge Valve or Multipurpose Valve

Agilent InfinityLab LC Series VWD User Manual 20
1 Introduction to the Variable Wavelength Detector
Leak and Waste Handling
Figure 6 Infinity II Leak Waste Concept (Flex Bench installation)