Agilent Technologies InfinityLab LC Series User manual

Agilent InfinityLab LC Series
Variable Wavelength Detectors
User Manual
Agilent InfinityLab LC Series VWD User Manual

Agilent InfinityLab LC Series VWD User Manual
Notices
Document Information
Document No: SD-29000240
Part No: G7114-90001 Rev. C
EDITION 10/2019
Copyright
© Agilent Technologies, Inc. 2014-2019
No part of this manual may be repro-
duced in any form or by any means
(including electronic storage and retrieval
or translation into a foreign language)
without prior agreement and written con-
sent from Agilent Technologies, Inc. as
governed by United States and interna-
tional copyright laws.
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this document
is provided “as is,” and is subject to being
changed, without notice, in future edi-
tions. Further, to the maximum extent
permitted by applicable law, Agilent dis-
claims all warranties, either express or
implied, with regard to this manual and
any information contained herein, includ-
ing but not limited to the implied warran-
ties of merchantability and fitness for a
particular purpose. Agilent shall not be lia-
ble for errors or for incidental or conse-
quential damages in connection with the
furnishing, use, or performance of this
document or of any information con-
tained herein. Should Agilent and the user
have a separate written agreement with
warranty terms covering the material in
this document that conflict with these
terms, the warranty terms in the separate
agreement shall control.
Technology Licenses
The hardware and/or software described
in this document are furnished under a
license and may be used or copied only in
accordance with the terms of such
license.
Restricted Rights Legend
U.S. Government Restricted Rights. Soft-
ware and technical data rights granted to
the federal government include only
those rights customarily provided to end
user customers. Agilent provides this
customary commercial license in Soft-
ware and technical data pursuant to FAR
12.211 (Technical Data) and 12.212
(Computer Software) and, for the Depart-
ment of Defense, DFARS 252.227-7015
(Technical Data - Commercial Items) and
DFARS 227.7202-3 (Rights in Commer-
cial Computer Software or Computer
Software Documentation).
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could result
in damage to the product or loss
of important data. Do not proceed
beyond a
CAUTION notice until
the indicated conditions are fully
understood and met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in personal injury or death.
Do not proceed beyond a WARN-
ING notice until the indicated
conditions are fully understood
and met.

Agilent InfinityLab LC Series VWD User Manual 3
In This Book
This manual covers:
•the Agilent 1290 Infinity II Variable Wavelength Detector (G7114B) and
•the Agilent 1260 Infinity II Variable Wavelength Detector (G7114A).
Find information on other Agilent Variable Wavelength Detectors in separate
manuals.
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements, physical and
performance specifications.
3 Using the Module
This chapter explains the essential operational parameters of the module.
4 Preparing the Module
This chapter provides information on how to set up the detector for an analysis
and explains the basic settings.
5 Optimizing the Detector
This chapter provides information on how to optimize the detector.
6Troubleshootingand Diagnostics
Overview about the troubleshooting and diagnostic features.

Agilent InfinityLab LC Series VWD User Manual 4
7ErrorInformation
This chapter describes the meaning of detector error messages, and provides
information on probable causes and suggested actions how to recover from error
conditions.
8 Test Functions
This chapter describes the detector’s built in test functions.
9 Maintenance
This chapter provides general information on maintenance of the detector.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200 Infinity
Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent InfinityLab LC Series VWD User Manual 5
Contents
1 Introduction to the Variable Wavelength Detector 9
Introduction to the Detector 10
Product Description 11
Optical System Overview 13
Dual-Wavelength Mode 18
Leak and Waste Handling 19
Operating Principle 24
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 30
3 Using the Module 35
Magnets 36
Turn on/off 37
Status Indicators 39
Instrument Configuration 40
Set up the Detector with Agilent Open Lab ChemStation 42
The Detector User Interface 43
Detector Control Settings 45
Method Parameter Settings 46
Scanning with the VWD 50
Agilent Local Control Modules 52
4 Preparing the Module 54
Leak and Waste Handling 55
Setting up an Analysis 57
Solvent Information 64

Agilent InfinityLab LC Series VWD User Manual 6
5 Optimizing the Detector 69
Introduction 70
Match the Flow Cell to the Column 71
Set the Detector Parameters 74
Warm up of the Detector 75
6 Troubleshooting and Diagnostics 77
Available Tests versus Interfaces 78
Agilent Lab Advisor Software 79
7ErrorInformation80
What Are Error Messages 81
General Error Messages 82
Detector Error Messages 89
8 Test Functions 99
Introduction 100
Intensity Test 105
Cell Test 108
Wavelength Verification-Calibration 110
ASTM Drift and Noise Test 112
Quick Noise Test 113
Dark Current Test 115
Holmium Oxide Test 117
D/A Converter (DAC) Test 120
Other Lab Advisor Functions 123

Agilent InfinityLab LC Series VWD User Manual 7
9 Maintenance 124
Introduction to Maintenance 125
Warnings and Cautions 126
Overview of Maintenance 128
Cleaning the Module 129
Remove and Install the Doors 130
Replace the Deuterium Lamp 132
Replace the Flow Cell / Cuvette Holder 135
Repair the Flow Cells 137
Using the Cuvette Holder 141
Correcting Leaks 143
Replace Leak Handling System Parts 145
Replace the Module Firmware 146
10 Parts and Materials for Maintenance 147
Overview of Maintenance Parts 148
Standard Flow Cell 10 mm / 14 µl 150
Micro Flow Cell 3 mm / 2 µl 152
Semi-micro Flow Cell (Parts) 154
High Pressure Flow Cell (Parts) 156
Cuvette Holder (Parts) 157
Accessory Kit 158
11 Identifying Cables 159
Cable Overview 160
Analog Cables 162
Remote Cables 164
CAN Cable 168
RS-232 Cables 169
USB Cables 170

Agilent InfinityLab LC Series VWD User Manual 8
12 Hardware Information 171
Firmware Description 172
Electrical Connections 175
Interfaces 178
Setting the 6-bit Configuration Switch 186
Instrument Layout 190
Early Maintenance Feedback (EMF) 191
13 LAN Configuration 193
What You Have to Do First 194
TCP/IP parameter configuration 195
Configuration Switch 196
Initialization Mode Selection 197
Dynamic Host Configuration Protocol (DHCP) 199
Manual Configuration 202
14 Appendix 206
General Safety Information 207
Waste Electrical and Electronic Equipment (WEEE) Directive 216
Radio Interference 217
Sound Emission 218
Solvent Information 219
Declaration of Conformity for HOX2 Filter 220
Agilent Technologies on Internet 221

Agilent InfinityLab LC Series VWD User Manual 9
1 Introduction to the Variable Wavelength
Detector
Introduction to the Detector 10
Product Description 11
Product Description G7114A 11
Product Description G7114B 12
Optical System Overview 13
Flow Cell 14
Lamp 15
Source Lens Assembly 15
Entrance Slit Assembly 15
Filter Assembly 16
Mirror Assemblies M1 and M2 17
Grating Assembly 17
Beam Splitter Assembly 17
Photo Diodes Assemblies 17
Photo Diode ADC (analog-to-digital converter) 17
Dual-Wavelength Mode 18
Leak and Waste Handling 19
Leak Sensor 23
Waste Concept 23
Operating Principle 24
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

Agilent InfinityLab LC Series VWD User Manual 10
1 Introduction to the Variable Wavelength Detector
Introduction to the Detector
Introduction to the Detector
The Agilent variable wavelength detectors described in this manual are designed
for highest optical performance, GLP compliance and easy maintenance with:
•higher data rate up to 120 Hz (G7114A) or 240 Hz for ultra-fast-HPLC
(G7114B),
•deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
•optional flow-cell cartridges (standard 10 mm, 14 µL; high pressure 10 mm,
14µL;micro3mm,2µL;semi-micro6mm,5µL)andPrepCellsareavailable
and can be used depending on the application needs (other types may be
introduced later),
•Dual wavelength mode, see “Dual-Wavelength Mode” on page 18.
•easy front access to lamp and flow cell for fast replacement,
•electronic identification of flow cell and lamp with RFID (Radio Frequency
Identification) tag for unambiguous identification,
•lamp information: part number, serial number, production date, ignitions,
burn time
•cell information: part number, serial number, production date, nominal
path length, volume, maximum pressure
•built-in electronic temperature control (ETC) for improved baseline stability,
and
•built-in holmium oxide filter for fast wavelength accuracy verification.