Amersham Biosciences AKTA design P-960 User manual

Pump P-960
18-1172-73
User Manual
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Important user information
All users must read this entire manual to fully
understand the safe use of Pump P-960.
WARNING!
The WARNING! sign highlights instructions
that must be followed to avoid personal
injury. Do not proceed until all stated
conditions are clearly understood and met.
Caution!
The Caution! sign highlights instructions that
must be followed to avoid damage to the
product or other equipment. Do not proceed
until all stated conditions are clearly
understood and met.
Note
The Note sign is used to indicate information
important for trouble-free and optimal use of
the product.
CE certification
This product meets all requirements in applicable CE-
directives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corresponding declaration of
conformity, is valid for the kit and instrument when:
– used as a stand-alone unit, or
– connected to other CE-marked Amersham
Biosciences instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from
Amersham Biosciences except for
alterations described in this manual.
WARNING!
This is a Class A product. In a domestic environment
this product might cause radio interference in which
case the user might be required to take adequate
measures.
Terms and Conditions of Sale
Unless otherwise agreed in writing, all goods and
services are sold subject to the terms and conditions of
sale of the company within the Amersham Biosciences
group which supplies them. A copy of these terms and
conditions is available on request.
Should you have any comments on this product, we will
be pleased to receive them at:
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Trademarks
Drop Design, UNICORN, ÄKTA and ÄKTAexplorer
are trademarks of Amersham Biosciences Limited.
Amersham and Amersham Biosciences are trademarks
of Amersham plc.
Office Addresses
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Amersham Biosciences UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Biosciences Corp.
800 Centennial Avenue
P.O. Box 1327
Piscataway, N.J. 08855
USA
Amersham Biosciences Europe Gmbh
Munzinger Strasse 9
D-79111 Freiburg
Germany
Amersham Biosciences KK
Sanken Building
3-25-1 Hyakunincho, Shinjuku-ku
Tokyo 169–0073
Japan
www.amershambiosciences.com
© Copyright Amersham Biosciences AB 2003
– All rights reserved
Pump P-960
User Manual 18-1172-73 Edition AA
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Pump P-960 User Manual 18-1172-73 Edition AA 5
Contents
1 Introduction
1.1 General ...................................................................................... 7
1.2 Safety ......................................................................................... 8
2 Installation
2.1 Unpacking .................................................................................. 9
2.2 General precautions .................................................................... 9
2.3 Installing the pump ..................................................................... 9
2.4 Connecting UniNet-2 cables ..................................................... 10
2.5 Connecting the tubing ............................................................... 11
2.6 Connecting the flow restrictor .................................................... 12
2.7 Initializing Pump P-960 in UNICORN ........................................ 13
2.8 Removing air from the sample flow path .................................... 13
2.9 Connecting an air sensor to Pump P-960 (optional) ................... 13
3 Operation
3.1 General .................................................................................... 14
3.2 User interface ........................................................................... 14
3.3 Starting and stopping the pump ................................................ 14
3.4 Setting the pressure limit .......................................................... 16
3.5 Calibration ................................................................................ 16
3.6 Filling the sample inlet tubings .................................................. 17
3.7 Ending the run and storage ....................................................... 18
3.8 Changing liquid ........................................................................ 18
3.9 Restart after power failure ......................................................... 18
4 Maintenance
4.1 Periodic maintenance ............................................................... 19
4.2 General care ............................................................................. 20
4.3 Cleaning-in-place ...................................................................... 20
4.4 Removing and installing the connection part ............................. 21
4.5 Replacing the connection part O-rings ...................................... 22
4.6 Cleaning and replacing the check valves ................................... 22
5 Troubleshooting
5.1 Error symptoms and corrective actions ...................................... 25
5.2 Removing trapped air bubbles from the sample pump .............. 28
6 Reference information
6.1 Description ............................................................................... 31
6.2 Cable connections .................................................................... 31
6.3 Liquid delivery .......................................................................... 32
6.4 Technical specifications ............................................................ 33
6.5 Ordering information ................................................................. 35
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6Pump P-960 User Manual 18-1172-73 Edition AA
Short instructions on back page
About this manual
This manual comprises two parts; a practical part (sections 1–5) and a
reference part (section 6). Sections 1–5 contain the necessary
information for installing, operating and maintaining the instrument.
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Introduction 1
Pump P-960 User Manual 18-1172-73 Edition AA 7
1 Introduction
1.1 General
Pump P-960 is a compact single-channel pump for use in liquid
chromatography and other applications where constant flow rate is
required. In ÄKTA™design chromatography systems, it is used as a
sample pump.
Pump P-960 features:
• Operating pressure up to 2.0 MPa (20 bar, 290 psi).
• Flow rates up to 50 ml/min.
• A pressure sensor connected to the pump outlet.
• Interface for connecting an air sensor directly to the pump.
Pump P-960 works with a wide range of columns and media supplied
by Amersham Biosciences.
The pump is controlled from a PC running UNICORN™control
system, version 4.12 or later.
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1Introduction
8Pump P-960 User Manual 18-1172-73 Edition AA
1.2 Safety
• The unit is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
• Do not block the outlet of the unit.
WARNING! HAZARDOUS CHEMICALS. When using hazardous
chemicals, all suitable measures, such as wearing protective glasses,
must be taken.
WARNING! CORROSIVE CHEMICALS. NaOH is corrosive and
therefore dangerous to health. Avoid spillage and wear protective
glasses.
WARNING! HAZARDOUS CHEMICALS. Incorrectly fitted tubing
might loosen, causing a jet of liquid to spray out. This is especially
dangerous if hazardous chemicals are being used. Connect the
tubing by first inserting the tubing fully, then tightening the connector
fingertight.
CAUTION! The mains power to the ÄKTAdesign chromatography
system must be switched OFF before connecting the unit to the
UniNet-2 link.
CAUTION! Always disconnect the UniNet-2 connectors before
attempting to replace any item on the unit.
CAUTION! Before the start of each run, always ensure that there is an
adequate supply of eluent in the reservoir. Avoid pumping without
liquid, since this might affect the lifetime of the sealing parts in the
pump.
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Installation 2
Pump P-960 User Manual 18-1172-73 Edition AA 9
2 Installation
2.1 Unpacking
Unpack the unit and check the items against the supplied packing list.
Inspect the items for obvious damage that may have occurred during
transportation.
Keep all packing materials if onward transport of the unit is expected.
2.2 General precautions
The unit should be installed in a non-corrosive atmosphere.
The unit should be located in a place of low temperature variations,
away from heat sources, draught and direct sunlight.
The unit may be operated at normal ambient temperature in the range
4 °C to 40 °C.
2.3 Installing the pump
When installing P-960 as an accessory, refer to the Optional
Configurations User Manual for detailed instructions on how to install
the sample pump in your ÄKTAdesign system.
CAUTION! Read the following information carefully to ensure that the
unit is installed correctly.
CAUTION! Make sure that the sample pump is installed and operated
at an upright position.
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2Installation
10 Pump P-960 User Manual 18-1172-73 Edition AA
2.4 Connecting UniNet-2 cables
Pump P-960 is controlled from a PC running UNICORN version 4.12
or higher via a UniNet-2 cable. Power is also supplied through this
cable.
The UniNet-2 chain connects, in series, Pump P-900/Pump P-920 with
Pump P-960 and other modules.
In ÄKTAdesign chromatography systems that include Pump P-960 as a
standard component, the UniNet-2 cables are installed at delivery.
To connect the UniNet-2 cable:
1 Remove the termination plug from the last component in the
UniNet-2 chain in the system.
2 Connect the supplied UniNet-2 cable
between the last component and the
UniNet-2 socket (the leftmost socket)
underneath Pump P-960.
The pump is now connected at the end of
the UniNet-2 chain. The termination
plug is not required since the sample
pump has an internal termination.
CAUTION! The mains power to the ÄKTAdesign chromatography
system must be switched OFF before connecting the unit to the
UniNet-2 link.
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Installation 2
Pump P-960 User Manual 18-1172-73 Edition AA 11
2.5 Connecting the tubing
If Pump P-960 is a standard component in the chromatography system,
the tubings are installed at delivery.
The exact tubing dimensions required for different sample application
techniques are described in the system documentation, or in the
ÄKTAdesign Optional Configurations User Manual.
2.5.1 Connection part to pressure
sensor tubing
This is a pre-bent tubing (PEEK,
i.d. 1.0 mm, o.d. 1/16"), which is
connected at delivery.
The tubing is connected with 1/16"
finger-tight fittings between one of
the connection part outlets and the
pressure sensor inlet.
A stop plug is connected to the other
connection part outlet.
2.5.2 Pump inlet tubing
1 Connect a 1/16" female/ M6 male union to the pump inlet.
2 Connect the pump inlet tubing (ETFE, i.d. 1.0 mm) to the union
using a 1/16" finger-tight connector.
Note: In ÄKTAexplorer™ systems, route this tubing through the
Connection part to pressure sensor tubing loop. Otherwise,
it might become entangled in nearby objects when closing
the door.
2.5.3 Pressure sensor outlet tubing
Connect the outlet tubing (ETFE, i.d. 1.0 mm) to the upper port on the
pressure sensor housing. Use a 1/16" finger-tight PEEK connector or a
connector with the same threading (UNF 10–32).
WARNING! HAZARDOUS CHEMICALS. Incorrectly fitted tubing
might loosen, causing a jet of liquid to spray out. This is especially
dangerous if hazardous chemicals are being used. Connect the
tubing by first inserting the tubing fully, then tightening the connector
fingertight.
Connection part to
pressure sensor
tubing
Pressure sensor
tubing
Pump inlet
outlet tubing
Connection part
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2Installation
12 Pump P-960 User Manual 18-1172-73 Edition AA
2.6 Connecting the flow restrictor
If the sample vessel is placed at a higher level than the end of the sample
waste tubing, sample might flow to waste. Flow restrictor FR-902
supplied is used in the sample flow path to eliminate this siphon effect
by creating a back-pressure of 0.2 MPa. Thereby, sample vessels can be
placed, for example, on top of the system.
The position of the flow restrictor in the flow path depends on how the
sample is applied with the sample pump (see Table 2-1). The injection
valve position refers to when sample otherwise might flow straight
through to waste.
Table 2-1. Position of the flow restrictor
When connecting the flow restrictor to the sample pump (Filling a
sample loop), use a 22 cm long tubing with suitable inner diameter
between the pump outlet and the flow restrictor IN port. Route the
tubing through the hole in the door and connect the flow restrictor
outside the door.
When connecting the flow restrictor to the injection valve, use a 10 cm
long tubing with suitable inner diameter between the flow restrictor IN
port and the injection valve.
Sample application type Flow restrictor port IN
connected to1
1A tubing should be used between the flow restrictor and the port. See
instruction below.
Injection valve
position
Filling a sample loop Sample pump P-960
outlet port
LOAD
Direct loading onto a column Injection valve V1, port 4 INJECT
Loading a Superloop2
2The flow restrictor is required only if filling the Superloop several times
during the run.
Injection valve V1, port 5 INJECT
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Installation 2
Pump P-960 User Manual 18-1172-73 Edition AA 13
2.7 Initializing Pump P-960 in UNICORN
1 Make sure that the correct strategy is installed.
2In
UNICORN Main menu, select Admininstration:System Setup.
3 Select the system used and click Edit.
4 Click Component.
5 Select Sample pump P-960 and click OK.
6 Click OK again and then Close to complete the initialization.
2.8 Removing air from the sample flow path
Note: Make sure that any air in the sample flow path is removed before
beginning to use Pump P-960. Air in the sample pump or in the
flow path might cause pressure pulsation and inaccurate flow.
The procedure for filling the sample flow path is decribed in section
5.2.2 Removing air using a purge tubing. You can also find this
information in ÄKTAdesign Optional Configurations User Manual.
2.9 Connecting an air sensor to Pump P-960 (optional)
Air sensor Air-912N (standard), Air-912, Air-925 or Air-925N can be
used at the sample inlet before the pump to prevent air from entering
the flow path.
The signal cable from Air sensor Air-912N (or Air-925N) is connected
directly to the air sensor socket underneath the pump. That minimizes
the time required to stop the pump when air is detected.
For detailed instructions on how to install the air sensor in your
ÄKTAdesign system, refer to the ÄKTAdesign Optional
Configurations User Manual.
2.9.1 Initializing the air sensor in UNICORN
1In
UNICORN Main menu, select Admininstration:System Setup.
2 Select the system used and click Edit.
3 Click Component.
4 Select Airsensor P-960 and click OK.
5 Click OK again and then Close to complete the initialization.
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3Operation
14 Pump P-960 User Manual 18-1172-73 Edition AA
3 Operation
3.1 General
Pump P-960 switches on automatically when the chromatography
system is switched on.
Pump P-960 is controlled from a PC running UNICORN version 4.12
or higher. It cannot be used as a stand-alone instrument. Control of the
pump can be achieved automatically from a method, or manually via
the functions available in UNICORN.
3.2 User interface
Pump P-960 is equipped with a green LED on the front panel. The LED
indicates the following:
• Flashing light indicates that the power is on.
• Steady light indicates that UniNet-2 is connected.
3.3 Starting and stopping the pump
3.3.1 Preparation before starting the pump
1 Make sure that all liquid used in the pump is filtered.
2 Place the liquid vessels on the workbench or on top of the system
then use the flow restrictor, see section 2.6 Connecting the flow
restrictor).
Do not close the vessels off completely. The pump might not work
if a vessel is sealed, or if it is placed too far below the pump inlet.
3 Check that there is enough liquid present for the run, and that the
ends of the inlet tubings are fully immersed. If no solvent filter is
used, fasten the tubings to keep the tubings ends at the bottom of
the liquid vessel. To change liquid, see section 3.8 Changing liquid.
CAUTION! Before a run, always make sure that there is a sufficient
supply of liquid in the reservoirs. Avoid pumping without liquid, since
this might affect the lifetime of the sealing parts in the pump.
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Operation 3
Pump P-960 User Manual 18-1172-73 Edition AA 15
4 Purge the pump before use as described in section 5.2 Removing
trapped air bubbles from the sample pump or in ÄKTAdesign
Optional Configurations User Manual.
5 Set the pressure limit as described in section 3.4 Setting the pressure
limit. If the pressure limit is exceeded, the pump is paused.
Note: When applying the sample directly onto the column, some
columns generate a back-pressure above 2.0 MPa (the upper
pressure limit of Pump P-960) at their recommended flow
rates. To maintain a back-pressure below 2.0 MPa when
using these columns, we recommend decreasing the flow
rate.
Note: We recommend decreasing the flow rate of the system pump
during equilibration, before sample application, and before
switching the sample pump in-line. The sample pump will
not start if the back-pressure exceeds 2.0 MPa.
3.3.2 Emergency stop
In case of emergency, switch off the power to the ÄKTAdesign system.
3.3.3 Setting the flow rate, starting and stopping the pump
To set the flow rate and start the pump manually:
1IntheSystem Control module, select Manual:Pump.
2 Select the instruction SampleFlow_960 in the list.
3 Type the desired flow rate.
4 Click on the Execute button. The pump starts.
5 To stop the pump, set the flow rate to 0 and click on Execute.
The pump can be run without liquid, but this should be avoided since
it might increase the mechanical wear.
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3Operation
16 Pump P-960 User Manual 18-1172-73 Edition AA
3.4 Setting the pressure limit
When applying the sample directly onto the column, it is important to
set the upper pressure limit of the sample pump according to the
pressure specification of the column that is used.
To set the upper pressure limit:
1In
System Control, select Manual:Alarm&Mon.
2 Select the Alarm_SamplePressure_P960 in the instruction list.
3 Select the Enabled mode.
4 Type the desired pressure limit in the HighAlarm field.
5 Click on the Execute button.
3.5 Calibration
The sample pump is calibrated at the factory.
If pressure pulsation or flow inaccuracy occur, first remove any trapped
air from the sample flow path as described in section 5.2 Removing
trapped air bubbles from the sample pump. Alternatively, follow the
instruction for your configuration in the ÄKTAdesign Optional
Configurations User Manual.
If the flow rate remains out of specification, contact the local
Amersham Biosciences service representative.
3.5.1 Calibrating the pressure offset
To calibrate the pressure offset:
1 Expose the pressure sensor to atmospheric pressure only.
2IntheSystem Control module in UNICORN, select System:Calibrate.
3 Select P960Press.
4 Click on the Start Calibrate button.
The system adjusts the pressure offset of the pump.
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Operation 3
Pump P-960 User Manual 18-1172-73 Edition AA 17
3.6 Filling the sample inlet tubings
We recommend using a purge tubing for filling the sample inlet tubings
and the sample pump. When the tubings are empty or the pump is dry,
the purge tubing must be used.
The procedure for using the purge tubing is described in section
5.2.2 Removing air using a purge tubing.
3.7 Using samples with high viscosity
When using samples with high viscosity (4–5 cP) at flow rates above
25 ml/min, it is necessary to use teflon tubings with i.d. 1.6 mm and
o.d. 1/8" as inlet tubings to Air-912N and Pump P-960.
When using samples with very high viscosity (up to 10 cP) the
maximum flow rate is 10 ml/min. We also recommend using teflon
tubings with i.d. 1.6 mm and o.d. 1/8" as inlet tubings to Air-912N and
Pump P-960.
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3Operation
18 Pump P-960 User Manual 18-1172-73 Edition AA
3.8 Ending the run and storage
If no further runs are planned, Pump P-960 should be flushed
immediately with at least 50 ml of pure, distilled water. If aqueous
buffers have been in use, this is particularly important to prevent salt
precipitation.
Note: If buffers or water are stored at room temperature, there is a risk
that bacterial growth might occur.
3.8.1 Weekend and long-term storage
Flush the pump with at least 50 ml of pure and distilled water and then
fill it with 20% ethanol.
3.9 Changing liquid
When changing from one liquid to another, it is important that the two
liquids are totally miscible with one another. If the two are immiscible,
the pump should be flushed first with an intermediate liquid, which is
miscible with both liquids. Failure to do so will disrupt the flow from
the pump.
3.10 Restart after power failure
If the power to the pump is interrupted, it automatically performs a
selftest when the power is restored. The calibration values are retained.
The default settings for flow rate and pressure limit are reset
automatically. The pump is then ready for operation.
CAUTION! To prevent precipitation of crystals when changing from a
salt-containing buffer to an organic solvent, always flush the pump
with water as a intermediate liquid.
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Maintenance 4
Pump P-960 User Manual 18-1172-73 Edition AA 19
4 Maintenance
The flow path components in Pump P-960 have a limited lifetime,
which depends on the flow rate, pressure and eluents used.
4.1 Periodic maintenance
WARNING! Always disconnect the power supply before attempting to
replace any item on the unit.
WARNING! HAZARDOUS CHEMICALS. Incorrectly fitted tubing
might loosen, causing a jet of liquid to spray out. This is especially
dangerous if using hazardous chemicals. Connect the tubing by first
inserting the tubing fully, then tightening the connector fingertight.
CAUTION! Only spare parts approved or supplied by Amersham
Biosciences may be used for maintaining and servicing the pump.
Interval Action (see procedures below)
Daily General care
When required Replace the O-rings in the connection part.
Clean the check valves. If necessary, replace the
O-rings or the check valves.
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4Maintenance
20 Pump P-960 User Manual 18-1172-73 Edition AA
4.2 General care
Inspect the pump daily for liquid leaks.
If you suspect that air bubbles are trapped in the sample flow path, go
through the air removal procedure for your configuration described in
section 5.2 Removing trapped air bubbles from the sample pump, or in
ÄKTAdesign Optional Configurations User Manual.
4.2.1 General recommendations for all eluents
It is essential that all liquids passing through the pump are clean and
pure. Impure or dirty liquid might block the channels in the pump
assembly, and hence shortening their lifetime. Degassing prevents
formation of air bubbles, which can cause pressure pulsation and
inaccurate flow rate.
4.2.2 Additional recommendations for aqueous liquids
After a run with an aqueous liquid, the pump should always be
thoroughly washed with pure, distilled water to prevent salt
precipitation.
4.3 Cleaning-in-place
Pump a cleaning or sanitizing agent through the pump. The standard
recommendation is to use 1 M NaOH and then wash out immediately
with buffer and distilled water.
WARNING! CORROSIVE CHEMICALS. NaOH is corrosive and
therefore dangerous to health. Avoid spillage and wear protective
glasses.
CAUTION! NaOH might damage the wetted parts in the pump if left
for longer periods. After cleaning using NaOH, flush the pump
immediately with buffer and distilled water.
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