Beckman Coulter UniCel DxC 600 Installation and maintenance instructions

UniCel DxC, Customer, Daily Start Up Job Aid Page 1 of 15
Rev. F (September 2013) Beckman Coulter Professional Development
UniCel DxC 600/800
Daily Start Up
For Training Purposes Only
These job aids are shortened versions of procedures found in the reference below. Information
in the job aid is correct as of the date published. Verify you have the correct information.
References:
•UniCel®DxC Synchron Systems Instructions for Use PN A93719AB (October 2012)

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Rev. F (September 2013) Beckman Coulter Professional Development
UniCel DxC 600/800 Synchron Clinical Systems
WARNINGS AND PRECAUTIONS
Read all product manuals and consult with Beckman Coulter-trained personnel before
attempting to operate the instrument.
Beckman Coulter, Inc. urges its customers and employees to comply with all national
health and safety standards such as the use of barrier protection. This may include,
but is not limited to, protective eyewear, gloves, suitable laboratory attire when
operating or maintaining this or any other automated laboratory equipment.
INTENTION FOR USE
This document is not intended to replace the information in your Instrument
Instructions for Use Manual (IFU). Information in the Instructions for Use Manual
supersedes information in any other manual.
REVISION STATUS
Rev. F (September 2013)
Based on DxC software version 5.3 (or higher)
TRADEMARKS
Beckman Coulter, the stylized logo, Access, Microtube, Synchron, and UniCel are
trademarks of Beckman Coulter, Inc., and are registered with the USPTO. All other
trademarks are the property of their respective owners.

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Rev. F (September 2013) Beckman Coulter Professional Development
DxC Daily Start Up
Flowcharts Page
Clear Manually Assigned Racks 5
Check Reagent Status 6
Load MC Reagents 7
Load CC Reagents 8
Unload CC Reagents 9
Check Calibration Status 10
Calibrate 11
Process Quality Control using Auto Generation of
Control 12
Manually Program/Process Quality Control 13
Sample Processing 14
Low Volume Sample Processing 15

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Rev. F (September 2013) Beckman Coulter Professional Development

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Rev. F (September 2013) Beckman Coulter Professional Development
Clear Manually Assigned Racks
Select Samples from the menu bar
Select the Rack(s) field. Type the range of
rack numbers(s) to be cleared
Note: Enter 1-999 to clear all racks
Notes about Clearing Racks
Clearing Racks does not clear programming for Sample ID’s; it only
clears the assignment to a rack/position. Your Closed Tube Tracking
Database tracks which samples have been pierced by Sample ID.
You may get a message informing you that some racks are In-Progress,
Removed, Requested for calibration, or Reserved for Cal. This feature
protects you from inadvertently clearing a rack that is needed for one of
these conditions.
Select Clear F7
Select Enter
from the keyboard
Select OK
Select OK
to confirm

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Rev. F (September 2013) Beckman Coulter Professional Development
Check Reagent Status
Select Print F10
Note: Reagent status can also be viewed on the software
Review the Vol (%)/Tests Left, Days Left,
and Reagent Status columns
for reagent requirements
Select Rgts/Cal from the menu bar

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Rev. F (September 2013) Beckman Coulter Professional Development
Load MC Reagents

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Rev. F (September 2013) Beckman Coulter Professional Development
Load CC Reagents
Yes
Select Rgts/Cal from the menu bar
Page down to locate the position(s) on the CC carousel where
reagent cartridge(s) will be placed (pages 3 to 8)
When prompted by the software, open the reagent carousel door
Prepare reagent(s) if required
Procedure complete
Place cartridge in the load position in front of the blinking bar
code reader beam OR
Enter Reagent name (Chem)
Using the picture on the right as
a reference, enter the following:
Lot number (numbers only)
Serial number
Expiration date (MM/YY)
Select the Tab key on the
keyboard to save the entries
Verify the reagent information is displayed on the monitor
Move the cartridge onto the reagent carousel
Close the reagent carousel door
Additional
reagents
selected?
No
Select Load F1
Select all Positions to load
reagent cartridges
Remove caps, remove any liquid adhering to the chimneys of the
cartridge and remove any bubbles on the surface of the reagent

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Rev. F (September 2013) Beckman Coulter Professional Development
Unload CC Reagents
Yes
No
Select Rgts/Cal from the Menu bar
Page down to locate the position(s) on the CC carousel
where reagent cartridge(s) will be unloaded (pages 3 to 8)
When prompted by the software,
open the reagent carousel door
Procedure complete
Grasp the cartridge in the load position (located at the
blinking bar code reader) and move it in front of the barcode
reader beam or select Clear F1 to remove reagent
information. Verify the reagent information is removed from
the monitor. Remove the cartridge
Close the reagent carousel door
Additional
reagents
selected?
Select Load F1
Select all Positions to
unload reagent cartridges

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Rev. F (September 2013) Beckman Coulter Professional Development
Check Calibration Status
Select Print F10
Note: Calibration status can also be viewed on the software
Review the Cal Time Left and Cal Status
columns to identify reagents requiring
calibration
Select Rgts/Cal from the menu bar

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Rev. F (September 2013) Beckman Coulter Professional Development
Calibrate

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Rev. F (September 2013) Beckman Coulter Professional Development
Process Quality Control using Auto Generation of Control

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Rev. F (September 2013) Beckman Coulter Professional Development
Manually Program/Process Quality Control
No Yes
Select Samples from the menu bar
Manually Program Control
Type the Control ID in the Sample ID field
Select Enter on the keyboard
Optional: Enter rack/position number if a bar code
is not available
Select the chemistries to be run
Select Save F10
Repeat steps to program another level, if necessary
Pour the control material into sample cup(s). Nest the
sample cup(s) in the appropriate bar code labeled
tube(s) or place in assigned rack/position(s)
Procedure complete
When Quality Control is complete,
review for acceptability (defined by laboratory policy)
Note: If a manually programmed control is incomplete,
the system will only try to run the incomplete tests the
next time you run the Control ID. Clear the Control ID
to clear the incomplete QC sample and
reset Auto Generation of Control
Quality Control
acceptable?
Consult references for
troubleshooting assistance
Place the rack in the autoloader with the rack bar code
facing to the right
*Clear multiple IDs
by typing in each
Control ID
separated by a
comma
Clear Control IDs
Select Clear F7
Enter the Control ID(s) to be cleared in the
Sample ID(s) field*
Select Enter on the keyboard
Select OK
Select OK to confirm
Press the <RUN> button on the instrument

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Rev. F (September 2013) Beckman Coulter Professional Development
Sample Processing
Validated
CTS primary
tube?
No
Yes
Remove cap
Remove visible blood from the top of
capped tubes using a cotton tipped
swab moistened with DI water
Adequate
sample
volume*?
*Adequate
sample
volume
determined by
Primary Tube
Sample
Template
Use the Low Volume Sample
Processing flowchart
No
Is sample
programmed at
the LIS?
No
Yes
Place container in the
appropriate rack. Ensure
that bar code label, if
present, is visible through
the rack slot. Verify the
tube is seated properly
Load the prepared racks
on the autoloader with the
rack bar code facing to the
right
Press the <RUN> button
on the instrument
Select Samples
from the menu bar
Enter the Sample ID.
Enter Rack and Position if
readable bar code is not available
Select chemistries to be run
If necessary, select STAT check
box, Sample Type, Sample
Comment and/or Demog F2
Is there a
readable bar
code?
Select Samples
from the menu bar
Select Next F10
Enter the Sample ID. Enter Rack
and Position
Yes
Yes
No
Start Here
Select Next F10

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Rev. F (September 2013) Beckman Coulter Professional Development
Low Volume Sample Processing
Is sample
programmed at the
LIS?
Program chemistries
using instructions on
Sample Processing flowchart
Transfer the sample into a validated insert cup and
nest the cup in the bar coded primary tube.
Place in a Reserved rack
OR
Transfer the sample into a 0.5 mL sample cup
Note: Nest the 0.5 mL sample cup in a red metal
insert to reduce the dead volume to 20 µL.
Place the nested red metal insert in a Reserved
rack. Bar code label can be attached to the red
metal insert
Load the prepared rack(s) on the autoloader with
the rack bar code facing to the right
Press the <RUN> button on the instrument
No
Yes
Is there a
readable bar
code?
Yes
Select Samples
from the menu bar
Enter the Sample ID. Enter Rack
and Position
No
Select Next F10