Amersham Biosciences AKTAexplorer User manual

18-1139-59
Installation Guide
ÄKTAexplorer
18113959AC_TK.book Page i Monday, June 23, 2003 4:45 PM

18113959AC_TK.book Page ii Monday, June 23, 2003 4:45 PM

Important user information
All users must read this entire manual to fully
understand the safe use of ÄKTAexplorer.
WARNING!
The WARNING! sign highlights instructions
thatmustbefollowedtoavoidpersonalinjury.
Do not to proceed until all stated conditions
are clearly understood and met.
Caution!
The Caution! sign highlights instructions that
must be followed to avoid damage to the
product or other equipment. Do not to
proceed until all stated conditions are clearly
understood and met.
Note
The Note sign is used to indicate information
important for trouble-free and optimal use of
the product.
CE Certification
This product meets all requirements of applicable CE-
directives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corresponding declaration of
conformity is valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked Amersham
Biosciences instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from
Amersham Biosciences except for
alterations described in this manual.
WARNING!
This is a Class A product. In a domestic environment, it
may cause radio interference, in which case the user
may be required to take suitable measures.
Terms and Conditions of Sale
Unless otherwise agreed in writing, all goods and
services are sold subject to the terms and conditions of
sale of the company within the Amersham Biosciences
group which supplies them. A copy of these terms and
conditions is available on request.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Biosciences AB
SE–751 84 Uppsala
Sweden
Trademarks
Drop Design, ÄKTA, ÄKTAexplorer, Superloop and
UNICORN are trademarks of Amersham Biosciences
Limited.
Amersham and Amersham Biosciences are trademarks
of Amersham plc.
Microsoft and Windows are either registered
trademarks or trademarks of Microsoft Corporation in
the United States and/or other countries.
Office Addresses
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Amersham Biosciences UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Biosciences Corp.
800 Centennial Avenue
PO Box 1327
Piscataway NJ 08855
USA
Amersham Biosciences Europe GmbH
Munzinger Strasse 9
D-79111 Freiburg
Germany
Amersham Biosciences K.K.
Sanken Building
3-25-1 Hyakunincho, Shinjuku-ku
Tokyo 169–0073
Japan
Amersham Biosciences Limited
13/F., Tower I
Ever Gain Plaza
88 Container Port Road
Kwai Chung, New Territories
Hong Kong
www.amershambiosciences.com
© Copyright Amersham Biosciences AB 2003
- All rights reserved
18113959AC_TK.book Page iii Monday, June 23, 2003 4:45 PM

18113959AC_TK.book Page iv Monday, June 23, 2003 4:45 PM

Contents
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC v
Contents
1 About this installation guide ..........................7
2 Safety ..........................................................8
3 Pre-requisites .............................................10
4 Installation overview ...................................11
5 Installation of ÄKTAexplorer ........................12
5.1 Unpacking .................................................................................12
5.2 Installing the pH flow cell ...........................................................13
5.3 Installing column holders and tubing holders .............................14
5.4 Connecting tubings ....................................................................15
5.5 Installing the computer and the mains cable ..............................16
6 Installation test ..........................................18
6.1 Preparation of ÄKTAexplorer ......................................................18
6.2 Priming and purging Pump P-900 .............................................19
6.3 Preparing the installation test .....................................................21
6.4 Running the installation test method ..........................................22
6.5 Evaluating the installation test results .........................................25
6.6 Correcting faulty evaluation results .............................................28
7 Test record .................................................29
7.1 Gradient test result ....................................................................29
7.2 Step response test result ............................................................29
7.3 UV response test result ..............................................................29
8 Installation record ......................................30
9 Registration form ........................................31
9.1 Components ..............................................................................32
18113959AC_TK.book Page v Monday, June 23, 2003 4:45 PM

Contents
vi ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
18113959AC_TK.book Page vi Monday, June 23, 2003 4:45 PM

About this installation guide 1
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC 7
1 About this installation guide
ÄKTAexplorer™is assembled and fully tested before shipping.
For safe transportation, however, some components have been
detached and thus need to be remounted.
Cables, capillaries, accessories, column holder, etc. are enclosed in
paper boxes or in Box-900 located at the top of the instrument pile.
This guide describes how to install ÄKTA™explorer. The guide is
divided into two parts; one describing the installation and one
describing how to run the installation test. After the installation
procedure has been performed, your ÄKTAexplorer is ready for
purification work.
For full details of specifications, methods, maintenance, etc., refer to
the respective User Manuals and Instructions.
18113959AC_TK.book Page 7 Monday, June 23, 2003 4:45 PM

2Safety
8ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
2 Safety
•The system is designed for indoor use only.
•Do not use in a dusty atmosphere or close to spraying water.
Refer to Technical Specifications in the System Manual for detailed
environmental pre-requisites.
WARNING! The individual instruments must not be opened by the
user. They contain high voltage circuits that can give a lethal electric
shock.
WARNING! Monitor UV-900 uses high intensity ultra-violet light. Do
not disconnect the optical unit while the lamp is ON.
WARNING! ÄKTAexplorer must be connected to a grounded mains
socket.
WARNING! There must always be a sample loop or Superloop™
connected to ports 2 and 6 of the injection valve. This prevents liquid
spraying out of the ports when switching the valve, which is especially
dangerous if hazardous chemicals are being used.
WARNING! Two people are required to lift the system.
WARNING! Never block the ports on the outlet valve with stop plugs,
since this will create over-pressure and might result in injury.
WARNING! Only spare parts approved or supplied by Amersham
Biosciences may be used for maintaining and servicing the system.
18113959AC_TK.book Page 8 Monday, June 23, 2003 4:45 PM

Safety 2
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC 9
WARNING! If the system is turned around or the fraction collector
removed, the external capillaries and other tubings may become
entangled in nearby objects and be pulled from their connections
causing leakage.
WARNING! Never place waste containers on the top of the system. If
they become full and overflow, liquid may penetrate the system
causing a short-circuit.
WARNING! If heavier instrument components such as the pump are
removed from the rack and the door is then opened fully, the shift in
the centre of gravity of the system may cause it to tip over.
WARNING! Never place buffer containers on the top of the valve door.
If this is done, the containers may fall down when the valve door is
opened. Place the buffer containers on the buffer tray above Box-900.
WARNING! If the door is quickly pulled open to its full extent, the
internal capillary tubings may be pulled from their connections
causing leakage.
18113959AC_TK.book Page 9 Monday, June 23, 2003 4:45 PM

3Pre-requisites
10 ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
3 Pre-requisites
•Two people are required to lift ÄKTAexplorer onto the working
bench.
•To install ÄKTAexplorer, a working area of about 200 x 80 cm is
required.
•ÄKTAexplorer requires 100-120/220-240 V~, 50/60 Hz electrical
supply with safety grounding.
•Cutting-pliers are recommended for cutting plastic straps.
•A waste flask is needed.
•The installation test requires the following solutions:
- 1000 ml of distilled water for priming and purging the pump.
- 500 ml of 0.4% acetone in distilled water.
- 100 ml of 20% ethanol in distilled water.
WARNING! ÄKTAexplorer must be connected to a grounded mains
socket.
18113959AC_TK.book Page 10 Monday, June 23, 2003 4:45 PM

Installation overview 4
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC 11
4 Installation overview
•Unpack ÄKTAexplorer ...........................................................12
•Detach packing material, and install items enclosed.................12
•Unpack and install the computer..............................................16
•Connect mains power cabling..................................................17
•Connect UniNet-1 data communication chain cabling.............16
•Complete the first two sections of the installation record.........30
•Prepare ÄKTAexplorer for the installation test ........................18
•Run the installation test method ..............................................22
•Evaluate the gradient ...............................................................27
•Evaluate the step response .......................................................27
•Evaluate the UV response ........................................................27
•Complete the test record..........................................................29
•Complete the registration form................................................31
•Complete the final section of the installation record ................30
•Store photocopies of all records and forms in the System Logbook.
•Store the Installation Guide in the User Manual box.
18113959AC_TK.book Page 11 Monday, June 23, 2003 4:45 PM

5Installation of ÄKTAexplorer
12 ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
5 Installation of ÄKTAexplorer
Begin by creating a clean and dry working area of 200 x 80 cm that
allows easy access. Then follow the step-by-step instructions below and
fill in the installation record as you go along, see page 30.
Note: Some components are packed in Box-900, located at the top of
the system.
Note: Some packing lists are included in the paper boxes.
5.1 Unpacking
1 After havingremoved thecardboard hood,
check the contents against the attached
packing list.Check alsoall includedboxes.
Store all the enclosed paper boxes and
plastic bags in a convenient nearby place.
2 Release and remove the red strap (1)
holding the system to the pallet.
3 Lift ÄKTAexplorer onto the work area
using the four strap handles (2). Two
people are required to lift the system. Do
not raise to upright position yet. Lay the
system on the same side as on the pallet.
4 Release the two red straps with the strap
handles. Allow the straps to remain taped
to the plastic cover.
5 Pull back the plastic cover (3) to
uncover the swivel platform (4) and
raise the system to an upright
position.
6 Remove the plastic cover with the
red straps from the system.
7 Save all the original packing
material. If, for any reason, the equipment has to be repacked, for
transportation or otherwise, it is important that the system can be
safely packed using the original packing material.
1
2
4 3
18113959AC_TK.book Page 12 Monday, June 23, 2003 4:45 PM

Installation of ÄKTAexplorer 5
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC 13
5.2 Installing the pH flow cell
1 Install the pH flow cell holder (packed in Box-900) in the groove
directly below the outlet valve (V4) on the outside of the valve door.
2 Push the slide clamp upwards to secure the pH flow cell holder.
3 Connect capillary G12 to the inlet of the pH flow cell.
4 Connect the outlet of the pH flow cell to the FR-904 flow restrictor
(marked IN) using the G13 capillary.
5 Connect the outlet of the FR-904 flow restrictor to the centre port
of the V4 outlet valve using the G14 capillary.
6 Place the FR-904 flow
restrictor (14) in the hole in
the pH flow cell holder.
7 Mount the pH electrode (16),
according to the figure, for
experiments where pH is to be
measured. Otherwise, mount
the pH electrode dummy in
the hole in the pH flow cell
holder.
Note: Donotinstall the pH electrodeuntilthe installationtesthasbeen
performed.
Note: Whenusing thepHelectrode,the flowrestrictor FR-904must be
replaced with the supplied flow restrictor FR-902. Otherwise,
the long term stability and lifetime of the pH electrode will
deteriorate.
Note: For calibration of the pH electrode, refer to the
UNICORN™User Manual.
MonitorP910
MonitorP910
MonitorP910
ÄKT
A
explorer
G12
14
16
18113959AC_TK.book Page 13 Monday, June 23, 2003 4:45 PM

5Installation of ÄKTAexplorer
14 ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
5.3 Installing column holders and tubing holders
1 Install the column holders (17a, 17b packed in Box-900) onto the
valve door. They should be placed close to the column selection
valves (V2 and V3).
Note: To release the column holders, press the slide clamp (18a, 18b)
downward.
2 Install the tubing holder (19), the tube holders (20) and the sample
tray (21) by pushing them into the slits below the
sample valve (V5).
18a
17a
17b
18b
MonitorP910
MonitorP910
MonitorP910
ÄKT
A
explorer
21
23
19
20
24
MonitorP910
MonitorP910
MonitorP910
ÄKTA
explorer
18113959AC_TK.book Page 14 Monday, June 23, 2003 4:45 PM

Installation of ÄKTAexplorer 5
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC 15
3 Locate the sample pump waste tubing (23) penetrating the valve
door. Remove the wire clips (22) from the tubing and place the
outlet in an E-flask (24) placed on the sample tray.
Note: For calibration of the sample pump, refer to the Pump P-960
User Manual.
5.4 Connecting tubings
1 Turn ÄKTAexplorer on its swivel platform to access the rear and
remove the plastic bag from the inlet tubings marked A11, A2, B1
and B2.
2 Locate the buffer valve (V6) and connect the tubing marked A1
(25) to the central port of the buffer valve.
21
23
19
20
24
22
MonitorP910
MonitorP910
MonitorP910
ÄKTA
explorer
25
26
18113959AC_TK.book Page 15 Monday, June 23, 2003 4:45 PM

5Installation of ÄKTAexplorer
16 ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
3 Snap the inlet tubings A11, A2, B1 and B2 into the tubing holder
(26) located at the upper left.
4TurnÄKTAexplorer on its swivel platform to access the valve door.
5 Remove the wire clips from the waste tubing connected to port 5
(29) on the injection valve (V1).
6 Place the wastetubing in a waste bottle and placethe bottle infront
of ÄKTAexplorer or where convenient.
Note: The installation of a fraction collector (optional) is described in
detail in the ÄKTAexplorer Optional Configurations User
Manual. Refer to the section that describes your fraction
collector.
5.5 Installing the computer and the mains cable
Unpack and install the computer and printer according to the
manufacturer’s instructions. Place them to the left of thesystem. Do not
switch them on!
1 Complete the UniNet-1 data
communication chain by
connecting a UniNet-1 cable
between the computer and
Pump P-900.
MonitorP910
MonitorP910
MonitorP910
ÄKTA
explorer
29
CAUTION! The mains power to ÄKTAexplorer must be switched OFF
before the UniNet-1 cabling is installed.
37
18113959AC_TK.book Page 16 Monday, June 23, 2003 4:45 PM

Installation of ÄKTAexplorer 5
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC 17
2 If using a fraction
collector, connect
UniNet 1 cable (35)
between Pump P-900 and
the fraction collector, and
UniNet 1 cable (36)
between the fraction
collector and the
computer.
3 All other UniNet-1 cables
are connected at delivery.
4 Connect a mains cable
(39) supplied between
ÄKTAexplorer and a
properly grounded
mains socket according
to the circled figure. Do
not switch on.
5 If using a fraction
collector, connect a
mains cable supplied
(38) between the
fraction collector and a
mainssocketatthe rear
of ÄKTAexplorer.
6 Complete the two first
sections of the Installation record on page 30.
7 The installation phase of ÄKTAexplorer is now completed.
CAUTION! The UniNet connection to the computer MUST be made
to the board with four LEDs (37).
35
36
Fraction
collector
(optional)
WARNING! ÄKTAexplorer must be connected to a grounded mains
socket.
To mains socket
To fraction
collector
39
38
Fraction
collector
(optional)
18113959AC_TK.book Page 17 Monday, June 23, 2003 4:45 PM

6Installation test
18 ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
6 Installation test
The installation test is designed to check the function of the liquid
delivery and the UV monitoring system of ÄKTAexplorer. The
installation test can also be used at any time to check the condition of
the system, e.g. after a prolonged stop.
Correct gradientformation is tested by producing a linear gradient and
a series of concentration steps of acetone.
Correct UV monitoring is tested by monitoring the acetone
concentration at 265, 254 and 280 nm and calculating the absorbance
ratios 265 nm/254 nm and 265 nm/280 nm.
6.1 Preparation of ÄKTAexplorer
6.1.1 Startup of the ÄKTAexplorer separation unit
1 Switch on the separation unit using the mains switch located to the
left on the base platform.
6.1.2 Startup of the computer and UNICORN software
1 Switchonthecomputer,thedisplayand theprinteraccordingtothe
instructions in the manufacturer manuals.
2 Log into Windows®by first pressing Ctrl-Alt-Del, and then clicking
OK.
3 When the Windows desktop appears, start UNICORN by double-
clicking on the UNICORN icon.
4 Select user default and enter default as password. Click OK.
Ä
KTA
explorer
Mains switch
18113959AC_TK.book Page 18 Monday, June 23, 2003 4:45 PM

Installation test 6
ÄKTAexplorer Installation Guide 18-1139-59 Edition AC 19
5 Click the System Control button in the taskbar.
6.2 Priming and purging Pump P-900
Prime the piston seal rinsing system of pump P-900 as follows:
1 Immerse the rinsing tubing in a flask (1) containing 20% ethanol in
distilled water.
2 Connect a syringe to the rinsing tubing that is connected
underneath (2) the left pump head on pump A. Slowly draw rinsing
solution to the syringe. When rinsing solution starts to enter the
syringe, continue to draw a few millilitres.
3 Loosen the syringe and put the tubing in the rinsing solution (1).
Purge the pump P-900 as follows:
1 Immerse the filters of inlet tubing A11 and B1 in the distilled water.
Note: Never place the reservoir flask below the level of the pump
inlet.
2 Connect a male Luer
syringeofabout30ml
to the open end of the
purge tubing.
3 Connect the male
Luer connector at the
other end of the purge
tubing to the left
purge valve at pump
module A.
4 Turn the purge valve
counter clockwise half a turn to open it andslowly draw eluent into
the syringe.
5 When fluid starts to enter the syringe continue to draw a few
millilitres before closing the purge valve. Check that there is no
visible air left in the inlet tubing.
6 Repeat steps 3 to 5 for all pump heads.
System Control button
AB
1
2
Purge valve
Outlet tubing
Purge tubing
18113959AC_TK.book Page 19 Monday, June 23, 2003 4:45 PM

6Installation test
20 ÄKTAexplorer Installation Guide 18-1139-59 Edition AC
6.2.1 Testing pressure stability
Perform a pressure test to establish that all air has disappeared from the
pump heads. Perform as follows:
1 Connect a column bypass capillary between the injection valve,
port 1, and the top of the UV optical unit.
2ÄKTAexplorer 10 (to check pump module A):
Run 0.2 ml/min at 0%B (distilled water). Check on the pump
display that the pressure reading is stable (variation < ±0.02 MPa).
ÄKTAexplorer 100 (to check pump module A):
Run 10 ml/min at 0%B (distilled water). Check on the pump
display that the pressure reading is stable (variation < ±0.2 MPa).
3ÄKTAexplorer 10 (to check pump module B):
Run 0.2 ml/min at 100%B (distilled water). Check on the pump
display that the pressure reading is stable (variation < ±0.02 MPa).
ÄKTAexplorer 100 (to check pump module B):
Run 10 ml/min at 100%B (distilled water). Check on the pump
display that the pressure reading is stable (variation < ±0.2 MPa).
4 Proceed to step 5 if the pressure is stable. If not, consult the
Pump P-900 User Manual for troubleshooting instructions.
5Click
END.
18113959AC_TK.book Page 20 Monday, June 23, 2003 4:45 PM