Syngene NuGenius User manual

NuGenius/NuGenius+ User manual
USER MANUAL

NuGenius/NuGenius+ User manual
Contents Page
SAFETY PRACTICES........................................................................................................................................................ 4
CHAPTER ONE - INTRODUCTION ..................................................................................................................................12
1.1 APPLICATIONS SUPPORTED .......................................................................................................................................... 12
1.2 HARDWARE.............................................................................................................................................................. 12
1.2.1 Specification................................................................................................................................................ 12
1.2.2 System Components ................................................................................................................................... 13
1.2.3 Accessories.................................................................................................................................................. 14
CHAPTER TWO - INSTALLATION ...................................................................................................................................15
2.1 INSTALLATION........................................................................................................................................................... 15
2.1.1 Transilluminator set-up............................................................................................................................... 15
2.2.2 Power on/off............................................................................................................................................... 15
2.2 USER INTERFACE ....................................................................................................................................................... 15
2.3 DYNAMIC FIELD CORRECTION........................................................................................................................................ 16
2.4 EXTENDED DYNAMIC RANGE (EDR).............................................................................................................................. 16
2.5 SELECT REGIONAL POWER FREQUENCY .......................................................................................................................... 16
2.6 INSTALLING GENETOOLS ON A SEPARATE PC................................................................................................................... 16
CHAPTER THREE - IMAGE ACQUISITION.......................................................................................................................17
3.1 CAPTURING AN IMAGE OF AN AGAROSE ETBR OR SIMILAR GEL ............................................................................................ 17
Step 1 –Position sample and choose lighting ........................................................................................................... 17
Step 2 –Adjust lens ................................................................................................................................................... 17
Step 3 –Set exposure time........................................................................................................................................ 17
3.2 CAPTURING AN IMAGE OF A PROTEIN GEL (COOMASSIE,SILVER STAINED)OR OTHER WHITE LIGHT IMAGE (COLONY PLATE,AUTORAD,
MICROTITRE PLATE ETC)........................................................................................................................................................ 18
Step 1 –Position sample and choose lighting ........................................................................................................... 18
Step 2 –Adjust lens ................................................................................................................................................... 18
Step 3 –Set exposure time........................................................................................................................................ 18
3.3 CAPTURING AN IMAGE OF AN AGAROSE "SAFE"DYE GEL .................................................................................................... 19
Step 1 –Position sample and choose lighting ........................................................................................................... 19
Step 2 –Adjust lens ................................................................................................................................................... 19
Step 3 –Set exposure time........................................................................................................................................ 19
3.4 CAPTURING AN IMAGE OF A STAIN-FREE GEL (NUGENIUS+)............................................................................................... 20
Step 1 –Position sample and choose lighting ........................................................................................................... 20
Step 2 –Adjust lens ................................................................................................................................................... 20
Step 3 –Set exposure time........................................................................................................................................ 20
CHAPTER FOUR - SAVING / OPENING IMAGES AND PRINTING IMAGES .......................................................................21
4.1 SAVING IMAGES ........................................................................................................................................................ 21
4.2 OPENING IMAGES ...................................................................................................................................................... 21
4.3 PRINTING................................................................................................................................................................. 22
CHAPTER FIVE - IMAGE ENHANCEMENT ......................................................................................................................23
5.1 IMAGE ENHANCEMENT ............................................................................................................................................... 23
5.2 ANNOTATION ........................................................................................................................................................... 24
5.3 DIGITAL ZOOM.......................................................................................................................................................... 24
CHAPTER SIX - USER PREFERENCES ..............................................................................................................................26
6.1 SETTINGS ................................................................................................................................................................. 26
6.2 STROBE COMPENSATION ............................................................................................................................................. 26
6.3 UV SAFETY............................................................................................................................................................... 26

NuGenius/NuGenius+ User manual
6.4 PRINTER…………………………………………………………………………………………………………………………………………………………. 27
6.5 DATE & TIME …………………………………………………………………………………………………………………………………………………. 28
CHAPTER SEVEN - TROUBLESHOOTING AND CONTACT INFORMATION .……………………………………………………………………. 29
7.1 TROUBLESHOOTING ….………………………………………………………………………………………………………………………………………... 29
7.2 CONTACT SYNGENE ..……………………………………………..…………………………………………………………………………………………… 29
APPENDIX A - LOOKING AFTER YOUR NUGENIUS/NUGENIUS+ SYSTEM.......................................................................30
APPENDIX B – DISPOSING OF YOUR IMAGINGSYSTEM .................................................................................................31

Safety Information
NuGenius/NuGenius+ User manual 4
Safety practices
This document describes the general safety practices and precautions that must be
observed when operating the NuGenius/NuGenius+.
This advice is intended to supplement, not supersede, the normal safety codes in the
user’s country. The information provided does not cover every safety procedure that
should be followed. Ultimately, maintenance of a safe laboratory environment is the
responsibility of the user and the user’s organization.
Please consult all documentation supplied with the NuGenius/NuGenius+ before you
start working with the instrument. Carefully read the safety information in this
document and in the other documentation supplied. When setting up the
instrument or performing analyses or maintenance procedures, strictly follow the
instructions provided.
Warning notices
We use ‘Warnings’ to highlight information or instructions that MUST be followed to
avoid personal injury to yourself or other people in the vicinity.
For example: Switch off the mains voltage and remove the mains cord before
cleaning.
Ensure that all instrument operators read and understand the precautions listed
below.
You are advised to post a copy of the precautions near or on the instrument shelf.
The following precautions must be observed when using the
NuGenius/NuGenius+.
Be sure that the voltage of the NuGenius/NuGenius+ instrument
corresponds to the voltage used in your laboratory.
Never remove the rear panel of the NuGenius/NuGenius+ instrument
without shutting down the instrument and disconnecting the instrument
power cord from line power.
The power cord must be an appropriately rated and approved cord-set in
accordance with the regulations of the country it is used in.
Do not replace the power cord with one of inadequate rating.

Safety Information
NuGenius/NuGenius+ User manual 5
Symbols
Symbol
Definition
Attention: See instructions for use.
Attention: Use of UV lights. Please Read UV Safety Warning.
Attention: Danger of electric shock.
Serial Number.
Symbol indicating "Not for general waste." For European
Union (EU) States, this symbol should be used to mark devices
that are reusable and not contaminated at the end of the
device life.
This symbol is a mandatory marking for devices entering the
European market to indicate conformity with the essential
health and safety requirements set out in European Directives.
Symbol for "Manufacturer." This symbol shall be adjacent to
the name and address of the manufacturer.
Symbol for "temperature limitation." The upper and lower
temperature limits will be indicated on either side of the
symbol.
Symbol used to indicate that the product should be kept dry.
Symbol indicating that the device is "fragile" and should be
handled with care.
Symbol indicating the correct upright position of the transport
package.

Safety Information
NuGenius/NuGenius+ User manual 6
Warning labels attached to the instrument draw your attention to specific
hazards. You must refer to this user guide and other documentation provided
with your system for more details concerning the potential hazard and any
precautions or other actions that must be taken.
Warning Labels
The following labels are displayed on the outer surfaces of the instrument:
UV Safety Warning
The NuGenius/NuGenius+ imaging system has a UV Transilluminator. If you open the
door the system will automatically switch the UV off. If you wish to override the UV,
please refer to section 6.3.
UV Warning
Electrical
Safety
Warning
Power
Rating and
Serial
Number
abel

Safety Information
NuGenius/NuGenius+ User manual 7
Before you override the UV safety interlock, read the following recommendations:
You should wear appropriate personal protection. As a minimum,
we recommend the use of full-face shields that meet the necessary
levels of UV protection. Those meeting the standard will be marked
with the ANSI Z87.1. notation [ANSI Z87.1-89, Practice for
Occupational and Educational Eye and Face Protection, IBR
approved for 29CFR1910.133(b)(1)].
We recommend that all users of UV be made familiar with the
guidelines published by the various national authorities. In the USA
these guidelines are published by OSHA (www.osha.gov) in their
standard 29CFR1910. This provides up to date safety information
and guidance. In the UK the NRPB (www.nrpb.org) issues similar
guidelines. Employers must familiarise themselves with these
guidelines and their obligations described in the standards.
In addition to face shields, we recommend that you should consider
wearing appropriate clothing to protect potential exposure to areas
of skin (face, arms, and hands for example).
N.B. The door safety interlock is reset when you close the door
General operating conditions
The NuGenius/NuGenius+ has been designed and tested in accordance with the
safety requirements of the International Electrotechnical Commission (IEC). The
NuGenius/NuGenius+ conforms to IEC61010-1 (Safety Requirements for electrical
equipment for measurement, control and laboratory use) as it applies to IEC Class 1
(earthed) appliances, and therefore meets the requirements of EC directive
2014/35/EU.
If possible, avoid any adjustment, maintenance or repair to the instrument while
it is open and operative. However, if any adjustment, maintenance or repair is
necessary while the instrument is open, this must be done by a skilled person
who is aware of the hazards involved.
Whenever circumstances arise that mean your NuGenius/NuGenius+ may be unsafe,
make it inoperative. In particular, a NuGenius/NuGenius+ may be unsafe if it:
Shows visible damage
Fails to perform the intended measurement
Has been subjected to severe transport stresses
Has been subjected to prolonged storage in unfavorable conditions

Safety Information
NuGenius/NuGenius+ User manual 8
Transportation and Storage Conditions
The system should only be transported and stored in its original packaging to ensure
maximum protection. It is recommended to keep the original packaging.
The unit should be transported and stored in an environment -10°C to +50°C, not
condensing.
If you must move the imaging system any great distance please contact your local
distributor to advise you about moving your system.
Environmental conditions
The instrument should only be used under the following conditions:
Indoors
Altitudes below 2000m
Ambient temperature between 5oC and 40oC
Relative humidity below 80% for temperatures up to 31oC, decreasing linearly
to 50% relative humidity at 40oC
Electrical supply fluctuations not exceeding +10% of the nominal voltage
The protection provided by the equipment may be impaired if the operating
conditions do not lie within these parameters.
The NuGenius/NuGenius+ instrument
requires two people to lift it safely.
The NuGenius/NuGenius+ weighs 20kg.

Safety Information
NuGenius/NuGenius+ User manual 9
Electrical safety
The instrument has been designed to protect the operator from potential electrical
hazards. This section describes some recommended electrical safety practices.
Lethal voltages are present at certain points within the instrument.
When the instrument is connected to line power, removing the instrument
covers is likely to expose live parts.
Even when the power switch is set to off, high voltages can still be present –
capacitors within the instrument may still be charged even if the instrument has
been disconnected from all voltage sources.
The instrument must be correctly connected to a suitable electrical supply. The
supply must have a correctly installed protective conductor (earth ground) and
must be installed or checked by a qualified electrician before connecting the
instrument.
Any interruption of the protective conductor (earth ground) inside or outside
the instrument, or disconnection of the protective conductor terminal is likely
to make the instrument dangerous.
Intentional interruption of the protective conductor is prohibited
Ensure that the electricity supply inlets on the instrument are not
obstructed, i.e. leave a gap to allow easy disconnection from the electricity
supply.
When working with the instrument:
Connect the instrument to a correctly installed line power outlet that has a
protective conductor connection (earth ground).
Do not operate the instrument with any covers or internal parts
removed.
Do not attempt to make internal adjustments or replacements except as
directed in the manuals.
Disconnect the instrument from all voltage sources before opening it for any
adjustment, replacement, maintenance or repair. If the opened instrument
must be operated for further adjustment, maintenance or repair, this must
only be done by your supplier’s Service Engineer.
Whenever it is possible that the instrument is no longer electrically safe
for use, make the instrument inoperative and secure it against any
unauthorised or unintentional operation. The electrical safety of the
instrument is likely to be impaired if, for example, the instrument:
-Shows visible damage
-Has been subjected to prolonged storage in unfavorable conditions

Safety Information
NuGenius/NuGenius+ User manual 10
-Has been subjected to severe stress during transportation
Electrical protection
Insulation: Class I rating for external circuits. Only connect equipment that
meets the requirements of IEC 61010-1, IEC 60950 or equivalent standards.
Installation Category: The instruments are able to withstand transient over
voltages typically present on the MAINS supply. The normal level of transient
over voltages is impulse withstand (overvoltage) category II of IEC 60364-4-
443.
Pollution Degree 2: Normally only non-conductive pollution occurs.
Occasionally, however, temporary conductivity caused by condensation
must be expected.
Electrical Specifications
The NuGenius/NuGenius+ power rating is as follows:
Voltage
100-240Vac
Frequency
50-60Hz
Current
2A
Fuse
2 of 20 mm x 5 mm IEC127 T 6.3 A H 250V
Changing fuses
There are two sets of fuses that you may need to replace: the first set is located
near the electrical power cord socket on the rear of the darkroom; the second
set is located on the rear of the UV Transilluminator inside the darkroom (if
supplied).
To change the fuses on the rear of the darkroom:
Switch off the darkroom and remove the line power cord from the electrical
supply.
Gently pull out the fuse holder on the rear of the darkroom:
Replace the fuse(s) with new ones of the same type and rating. The fuse
type is 20 mm x 5 mm IEC127 T 6.3 A H 250V for instruments in all
countries.
Replace the fuse holder.
Power socket
Fuse holder