Amersham Biosciences MegaBACE 500 Technical manual

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MegaBACE 500 and 1000
Planning Guide
Revision 6
December 2002
user manual
256-003
63004918 (SV# 399-326)
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AutoSeq, DYEnamic, MegaBACE, Sephadex, SNuPe, and Thermo Sequenase
are trademarks of Amersham Biosciences Limited.
Amersham and Amersham Biosciences are trademarks of Amersham plc.
ABgene and Thermo-Fast are trademarks of Advanced Biotechnologies Ltd.
CycleFoil, CyclePlate, CycleSeal, and Robbins Scientific are trademarks of
Robbins Scientific Corporation.
Dow Corning and Pyrex are trademarks of Dow Corning Corporation.
Ethernet is a trademark of the Xerox Corporation.
Liqui-Nox is a trademark of Alconox Inc.
Microseal is a trademark of MJ Research Inc.
Microsoft, Windows, and Windows NT are trademarks of Microsoft Corporation.
Pentium is a trademark of Intel Corporation.
Speed Vac is a trademark of Savant.
Texwipe is a trademark of Texwipe Company.
The polymerase chain reaction (PCR) is covered by patents owned by Roche
Molecular Systems and F Hoffmann-La Roche Ltd. A license to use the PCR process
for certain research and development activities accompanies the purchase of certain
reagents from licensed suppliers, such as Amersham Biosciences Limited and
affiliates, when used in conjunction with an authorized thermal cycler.
The PCR process for amplifying DNA is covered by US patent numbers 4,683,195
and 4,683,202 assigned to Hoffman-La Roche Inc and F Hoffmann-La Roche Ltd.
Patents are pending or issued in other countries.
The MegaBACE DNA Analysis System is for research purposes only. It is not intended
or approved for diagnosis of disease in humans or animals.
All goods and services are sold subject to the terms and conditions of sale of the
company within the Amersham Biosciences group that supplies them. A copy of
these terms and conditions is available on request.
© Amersham Biosciences Corp 2002
 All rights reserved.
December 2002
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Notice to Purchaser: Limited License
The MegaBACE instrument is a confocal scanning system licensed under
US Patent Numbers 5,091,652 and 5,274,240, and corresponding foreign patents
and patent applications, including any continuations, continuations-in-part, and
subdivisions and the like.
The instrument is also an Authorized DNA Sequencer. It is authorized under
one or more US Patent Numbers 4,849,513; 5,171,534; 5,015,733; 5,118,800;
5,161,507; 5,118,802; 4,855,225; and 5,366,860, and corresponding foreign
patents and patent applications. The purchase of this instrument includes limited,
non-exclusive rights under the subject patents to use this instrument for sequencing
and fragment length analysis when used with Authorized Reagents. The use of
this instrument with Authorized Reagents provides a limited license to perform
DNA sequencing and fragment length analysis in accordance with the label rights
accompanying such reagents. Purchase of this instrument does not itself convey
to the purchaser a complete license to perform DNA sequencing and fragment
length analysis under the subject patents. Authorized reagents may be obtained
from licensed vendors, or reagents may be authorized under separate license
arrangements from PE Applied Biosystems. The above patent rights are granted
solely for research and other uses that are not unlawful. No other licenses are
granted expressly, impliedly, or by estoppel.
Further information on purchasing licenses to perform DNA sequencing and
fragment length analysis may be obtained by contacting the Director of Licensing
at PE Applied Biosystems, 850 Lincoln Center Drive, Foster City, California 94404.
PE Applied Biosystems does not guarantee the performance of this instrument.
Amersham Biosciences Limited is a licensed vendor for authorized reagents.
The MegaBACE SNuPe genotyping kit is supplied under license from Orchid
BioSciences Inc and is covered by US patent numbers 5,888,819; 6,004,744,
and foreign equivalents.
The method for serially injecting multiple plates during a single run on a
capillary-array-electrophoresis instrument is covered by US patent number
6,156,178.
Amersham Biosciences UK Limited Amersham Place Little Chalfont
Buckinghamshire England HP7 9NA
Amersham Biosciences AB SE-751 84 Uppsala Sweden
Amersham Biosciences Corp 800 Centennial Avenue PO Box 1327 Piscataway
NJ 08855
USA
Amersham Biosciences Europe GmbH Munzinger Strasse 9 D-79111 Freiburg
Germany
Amersham Biosciences (SV) Corp 928 East Arques Avenue Sunnyvale
CA 94085-4520 USA
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MegaBACE 500 and 1000 Planning Guide ● v
Table of contents
Preface
About this guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Related publications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Special safety text . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Safety standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viii
Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viii
Chapter 1 Introduction
1.1 Preinstallation tasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.2 MegaBACE system components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.3 Additional recommended components . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.4 Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Chapter 2 Site requirements
2.1 Laboratory space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.1.1 Instrument space requirements. . . . . . . . . . . . . . . . . . . . . . . 2-1
2.1.2 Laboratory bench requirements. . . . . . . . . . . . . . . . . . . . . . . 2-3
2.2 Environmental requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.2.1 Temperature and humidity . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.2.2 Heat production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2.3 System electrical requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2.3.1 Uninterruptible power supply (UPS) . . . . . . . . . . . . . . . . . . . 2-5
2.3.2 Instrument and power supply fan module:
North America and Japan . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.3.3 Instrument and power supply fan module: Europe . . . . . . . . . 2-6
2.3.4 Computer and monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
2.4 Requirements for nitrogen cylinders and pressure regulators . . . . . . . 2-7
2.4.1 Regulator configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
2.4.2 Ordering information for the high- and low-pressure
nitrogen systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2.4.3 Requirements for the high-pressure nitrogen system . . . . . . . 2-9
2.4.4 Requirements for the low-pressure nitrogen system . . . . . . . 2-10
2.4.5 Checking the available nitrogen pressure. . . . . . . . . . . . . . . 2-10
2.4.6 Installing a nitrogen cylinder . . . . . . . . . . . . . . . . . . . . . . . . 2-10
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Table of contents
vi ● MegaBACE 500 and 1000 Planning Guide
Chapter 3 Emission filters, chemistry, and laboratory materials
3.1 Emission beamsplitters and filters. . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.1 Filter set for sequencing or SNP genotyping. . . . . . . . . . . . . . 3-1
3.1.2 Filter sets for STR genotyping . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.2 Chemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.3 Laboratory equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.4 Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.4.1 General consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.4.2 Additional consumables required for STR genotyping. . . . . . . 3-9
3.4.3 Additional consumables required for SNP genotyping . . . . . 3-10
Chapter 4 Planning for computer network and data storage
4.1 Basic MegaBACE system configuration. . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.2 Configuration for a high-throughput laboratory . . . . . . . . . . . . . . . . . . 4-2
4.3 Storage requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Chapter 5 Moving and reinstalling the instrument
5.1 Moving the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2 Required parts for reinstalling the instrument . . . . . . . . . . . . . . . . . . . 5-1
5.2.1 Major components and parts. . . . . . . . . . . . . . . . . . . . . . . . . 5-2
5.2.2 Power cords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
5.3 Connecting a new computer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Appendix A Preinstallation forms
A.1 Site information form . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
A.2 Preinstallation checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
Appendix B Training checklist
Appendix C Summary of laboratory space requirements
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MegaBACE 500 and 1000 Planning Guide ● vii
Preface
About this guide
The MegaBACE 500 and 1000 Planning Guide describes how to plan and
prepare your laboratory before you receive the MegaBACE™ DNA Analysis
System. This guide describes the requirements for the MegaBACE 500,
flexible MegaBACE 1000, and MegaBACE 1000 instruments.
• Chapters 1 through 5— Describe the system components, chemistry,
and site requirements.
• Appendixes— Provide preinstallation forms, checklists, and a summary
of the laboratory space requirements.
Related publications
In addition to the MegaBACE 500 and 1000 Planning Guide, the following
publications are available for the MegaBACE system:
• MegaBACE instrument user’s documentation provides safety information,
operation instructions, and maintenance and troubleshooting guidelines
for the instrument.
• MegaBACE instrument administrator’s guide provides information on
how to tailor the instrument control software for your laboratory and
applications.
• MegaBACE analysis software user’s guides provide instructions on
how to use the analysis software to analyze the data collected from the
MegaBACE instrument and guidelines for troubleshooting the software
and application.
Special safety text
Make sure you follow the precautionary statements presented in this guide.
Warning Indicates a possibility of severe or fatal injury to the user or other persons if the
precautions or instructions are not observed.
Caution Indicates that damage to the instrument, loss of data, or invalid data could occur
if the user fails to comply with the advice given.
Important Highlights information that is critical for optimal performance of the system.
Note: Identifies items of general interest.
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Preface
viii ● MegaBACE 500 and 1000 Planning Guide
Safety standards
The MegaBACE instrument complies with CE and other applicable
standards, such as UL and CSA. For the latest CE conformity information,
contact MegaBACE Technical Support. See Assistance for contact infomation.
Assistance
For questions regarding the planning and preparation of your laboratory,
contact MegaBACE System Technical Support.
When calling for assistance, be prepared to supply the serial number
of your instrument. The serial number is located on the lower right side
of the MegaBACE instrument. For contact by phone or fax, please use
one of the numbers below.
Asia Pacific Latin America
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Australasia Middle East and Africa
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Austria Netherlands
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Belgium Norway
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Canada Portugal
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Central, East, and Southeast Europe Russia & other C.I.S. & N.I.S.
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Denmark Southeast Asia
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Finland & Baltics Spain
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
France Sweden
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Germany Switzerland
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Italy UK
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Japan USA
Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone]
Web site
http://www.amershambiosciences.com
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MegaBACE 500 and 1000 Planning Guide ● p1-1
Chapter 1 Introduction
The MegaBACE DNA Analysis System (figure 1-1) is a high-throughput
automated gene analysis system. The system uses capillary array
electrophoresis to perform fragment separation on DNA samples and
then produces an electropherogram of the contents of each well in a
microplate. The analysis software allows you to perform detailed analysis
of the data.
This chapter provides a summary of—
• Preinstallation tasks (section 1.1)
• MegaBACE system components (section 1.2)
• Additional recommended components (section 1.3)
• Network (section 1.4)
Figure 1-1.
The MegaBACE system.
1000
100
0
1000
Power supply
fan module
Nitrogen cylinders*Laboratory bench*
* Not provided with the system.
(Optional)
Uninterruptible
power supply*
MegaBACE instrument
Monitor
Bar code
reader* Keyboard
and mouse
Computer
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Chapter 1 Introduction
p1-2 ● MegaBACE 500 and 1000 Planning Guide
1.1 Preinstallation tasks
The MegaBACE system includes unpacking and installation by Amersham
Biosciences. Before your MegaBACE system is installed, you should read this
planning guide and prepare your laboratory space. If you have questions or
concerns, contact MegaBACE System Technical Support. See Assistance in
the preface for contact information.
Warning Do not unpack or install the instrument and computer yourself. Only an Amersham
Biosciences representative should unpack and install the MegaBACE instrument
and computer. Improper unpacking and installation can cause personal injury
and damage to the system components.
Before your MegaBACE system is scheduled for installation, make sure you
complete the site information form and preinstallation checklist (appendix A).
See appendix B for the training checklist.
1.2 MegaBACE system components
The MegaBACE system consists of integrated hardware (figure 1-1), software,
reagents (chapter 3), and capillary arrays. The basic MegaBACE system
components are—
• MegaBACE instrument containing either one laser (488-nm argon-ion
blue laser) or two lasers (488-nm argon-ion blue laser and 532-nm green
solid-state laser)
• Pentium™ workstation that uses Microsoft™ Windows™ and hosts
the Instrument Control Manager and the analysis software.
Note: At a minimum, the MegaBACE software requires Windows 2000.
Call MegaBACE System Technical Support for information on the exact
configuration. See Assistance in the preface for contact information.
• Power supply fan module
• Capillary arrays
• Optical beamsplitter and filter set
• Accessory kit (cathode water tank, anode plugs, hoses, cables, fittings,
and tools)
• Other components (user documents and software CD)
• Installation and training
• One-year warranty
• Liability statement for the MegaBACE system
• (Optional) Service contract
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1.3 Additional recommended components
MegaBACE 500 and 1000 Planning Guide ● p1-3
1.3 Additional recommended components
The following components, which are not included as part of the basic
MegaBACE system, are recommended:
• Analysis workstation— Allows you to dedicate the provided workstation
for performing instrument runs. See chapter 4 for recommended
configurations.
• Uninterruptible power supply (UPS)— Protects the instrument from
power line fluctuations. See section 2.3.1 for details.
1.4 Network
The MegaBACE instrument and the workstation are connected using a
computer network—
• Instrument connection —PCI (peripheral component interconnect) SCSI
(small computer system interface) connection from the MegaBACE
instrument to the instrument control workstation
• Workstation network connection —10/100 Fast Ethernet™ adapter
See chapter 4 for recommended configurations. Contact MegaBACE System
Technical Support for specifications. See Assistance in the preface for contact
information.
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MegaBACE 500 and 1000 Planning Guide ● p2-1
Chapter 2 Site requirements
This chapter describes the site requirements for the MegaBACE system.
The topics include—
• Laboratory space requirements (section 2.1)
• Environmental requirements (section 2.2)
• System electrical requirements (section 2.3)
• Requirements for nitrogen cylinders and pressure regulators (section 2.4)
See appendix C for a summary of the laboratory space requirements.
2.1 Laboratory space requirements
This section describes the laboratory space and laboratory bench requirements.
The basic MegaBACE system includes one workstation for instrument control
and analysis. If you want additional workstations, you need to plan the space
accordingly. See chapter 4 for workstation configurations.
2.1.1 Instrument space requirements
The dimensions of the MegaBACE instrument are—
Width 1.033 m (40.7 in)
Height 0.812 m (32 in)
Depth 0.874 m (34.4 in)
When planning laboratory space for your MegaBACE instrument, you
should allow—
• 0.127 m (5 in) clearance for the laser exhaust port hose (figure 2-1)
• 0.127 m (5 in) clearance behind the instrument for service access
• 0.279 m (11 in) clearance above the instrument so that you can open
the electrophoresis chamber door
• Room for the power supply fan module
• Laboratory bench space for the computer
• Wall space for the nitrogen cylinders
• Space for an uninterruptible power supply (UPS) if you plan to use one
See figure 2-1 for details on space requirements.
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Chapter 2 Site requirements
p2-2 ● MegaBACE 500 and 1000 Planning Guide
Figure 2-1.
The MegaBACE system space requirements.
0.279 m
(11 in) 0.229 m
(9 in)
0.229 m
(9 in)
0.390 m
(15.3 in)
1000
100
0
1000
Power supply
fan module
Computer
(Optional)
Uninterruptible
power supply*
Laboratory bench*
MegaBACE instrument
Monitor
Bar code
reader* Keyboard
and mouse
0.812 m
(32 in)
0.279 m
(11 in)
Clearance for
chamber door
0.914 m
(36 in)
1.160 m
(45.7 in)
Laser exhaust port
1.524 mm
(60 in)
1.219 m (48 in) width by 0.914 m (36 in) depth minimum
Notes: 1. Allow 0.127 m (5 in) clearance behind the instrument for service access.
2. In addition to the components shown, the use of a UPS is recommended.
3. Computer and monitor dimensions can vary depending on the model provided.
* Not provided with the system.
0.432 m
(17 in)
0.563 m
(21 in)
Nitrogen cylinder
for high pressure*
Nitrogen cylinder
for low pressure*
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2.2 Environmental requirements
MegaBACE 500 and 1000 Planning Guide ● p2-3
2.1.2 Laboratory bench requirements
You must provide a level, stable laboratory bench or table space for the
MegaBACE instrument and hardware components. The bench or table
surface must be level within 0.635 cm over 0.914 m (0.25 in over 3 ft).
The laboratory bench or table you use must be rated to hold at least the
combined weight of the components (shown in figure 2-1). The approximate
weights of the components are—
MegaBACE instrument 272 kg (600 lb)
Computer monitor 005 kg (11.1 lb)
Computer tower 014 kg (30 lb)
Power supply fan module 026 kg (58 lb)
In addition, you should provide sufficient space to meet or exceed the
requirements defined in figure 2-1.
(For Europe only) A customized table capable of holding the weight of the
MegaBACE system is available for purchase. You can order the customized
table (product number 94-9900-28) from the following site:
Amersham Biosciences Europe GmbH
Munzinger Strasse 9, D-79111 Freiburg, Postfach 5480
D-790 21 Freiburg, Germany
[phone]
If the laboratory bench or table is not stationary, the legs should have a
locking mechanism. Contact MegaBACE System Technical Support for
a recommended supplier of benches that meet these requirements. See
Assistance in the preface for contact information.
2.2 Environmental requirements
This section describes the environmental requirements for the MegaBACE
instrument.
2.2.1 Temperature and humidity
For optimal performance of the MegaBACE instrument, the laboratory must
meet the following requirements:
Ambient temperature: 20–25 °C (68–77 °F)
Humidity: < 80% (noncondensing)
Pollution degree: 2
Installation category: II
Place the MegaBACE instrument away from heat and air conditioning ducts.
Do not place the instrument in direct sunlight.
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Chapter 2 Site requirements
p2-4 ● MegaBACE 500 and 1000 Planning Guide
2.2.2 Heat production
A standard power supply fan module is supplied with the instrument
(figure 2-1). If you vent the laser exhaust into the room, the rate of heat energy
production for each instrument is approximately 10 300 BTU. To reduce heat
buildup in the instrument room to approximately 4 100 BTU per instrument,
you can vent the exhaust into the ceiling space or outside the building if local
safety and building codes permit.
If you vent the exhaust into the ceiling space, make sure that your building
ventilation system can handle the approximately 6 200 BTU of heat energy
that is vented. You should also take into account that the computer and
monitor produce approximately 2 800 BTU of heat energy, depending on
the model provided.
To reduce noise in the instrument room, you can use an external exhaust
fan. Contact MegaBACE System Technical Support for more information.
See Assistance in the preface for contact information.
You can choose to vent the hot air out the back of the power supply fan
module into a duct. To do this requires a 0.102-m (4-in) flange. Contact
MegaBACE System Technical Support for details.
The airflow requirement is 1.557 m
3
/min (55 ft
3
/min) minimum at
0.038 m (1.5 in) H
2
O static pressure at the laser exhaust port on the
instrument (figure 2-1).
2.3 System electrical requirements
The MegaBACE system includes four components that require electrical
power: the instrument, the power supply fan module, the computer, and
the monitor (sections 2.3.2 through 2.3.4). Four electrical power cords
are included with each system, one for each of these main components.
The use of a UPS is recommended to protect the instrument from power
line problems (section 2.3.1).
Warning Use only the power cords supplied with the MegaBACE system. Make sure
the cords are in good condition and are not frayed. Use of incorrect power cords
can cause damage to the instrument. Use of frayed or damaged power cords can
cause injury.
Important You must locate the right side of the MegaBACE instrument within 2.5 m (8 ft)
of the electrical outlets.
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2.3 System electrical requirements
MegaBACE 500 and 1000 Planning Guide ● p2-5
2.3.1 Uninterruptible power supply (UPS)
Caution Without the recommended UPS, the instrument is vulnerable to power line
fluctuations.
In the event of a power failure, depending on the duration, the UPS might
not contain enough stored power to finish a run that is in process and allow
the capillaries to be stored properly.
Amersham Biosciences recommends the use of a UPS rated for at least 4 kVA.
Using a UPS protects the instrument, the capillaries, and your data from
damage or loss caused by unexpected power failures, surges, or AC line
fluctuations. The UPS also acts as a power line regulator, line conditioner,
and surge suppressor and works to protect against all power line problems.
If you are using a UPS, you must connect the instrument, power supply fan
module, computer, and monitor to the UPS. If you have an external blower
for laser cooling, you must also connect the blower to the UPS.
Contact MegaBACE System Technical Support for information about a
qualified UPS. See Assistance in the preface for contact information.
Note: A UPS will most likely require electrical connections at the wall that
differ from those of the MegaBACE instrument. Contact the UPS manufacturer
or MegaBACE System Technical Support for details concerning site
preparation when using a UPS. See Assistance in the preface for contact
information.
2.3.2 Instrument and power supply fan module:
North America and Japan
The MegaBACE instrument and power supply fan module are designed
to run at 200–240 V
~
worldwide. In North America and Japan, the instrument
and power supply fan module require a 200–220 V
~
circuit with a 20 A circuit
breaker. See table 2-1 for detailed voltage requirements.
Table 2-2 lists the plug and receptacle configurations that can be used for
the MegaBACE instrument, power supply fan module, and computer. The
MegaBACE system includes two power cords with standard NEMA 6-15P
plugs for the instrument and power supply fan module and two 5-15P plugs
for the computer and monitor.
Table 2-1.
Electrical requirements for the MegaBACE instrument and the power
supply fan module
Location Volts (AC) Frequency Amps
US/Canada 208 ±10% or 220 ±10% 60 Hz ±1% 15
Japan 200 ±10% 50/60 Hz ±1% 16
Europe 230 ±10% 50 Hz ±1% 14
UK and Australia 240 +6% or -10% 50 Hz ±1% 13
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Chapter 2 Site requirements
p2-6 ● MegaBACE 500 and 1000 Planning Guide
You must provide a 20 A grounded circuit that terminates with two 6-15R
or 6-20R receptacles. Termination can be at the wall, from a power strip,
or at the output of a UPS. The 6-20R receptacle will accept either the
6-15P or 6-20P plug.
Note: The receptacles are not provided. Contact MegaBACE System Technical
Support for purchasing information. See Assistance in the preface for contact
information.
2.3.3 Instrument and power supply fan module: Europe
The MegaBACE instrument and power supply fan module are designed to
run at 200–240 V
~
worldwide. (See tables 2-1 and 2-2 for detailed voltage
and outlet requirements.) The MegaBACE system includes two power cords
with European standard CEE 7/7 (“Schuko”) plugs for the instrument and
power supply fan module. This configuration allows the use of standard
plugs and receptacles that are found in continental Europe.
Use of the recommended UPS (section 2.3.1) requires special high-current
electrical hookups. Please refer to the manufacturer’s guidelines and follow
all applicable local electrical codes during the site preparation. Contact the UPS
manufacturer or MegaBACE System Technical Support for more information.
See Assistance in the preface for contact information.
Caution Without the recommended UPS, the instrument is vulnerable to power line
fluctuations.
If you do not use a UPS, you can plug the MegaBACE instrument and the
power supply fan module into separate grounded circuits capable of delivering
at least 10 A each at 230 V
~
, using the standard country-accepted outlet.
Alternatively, you can use a single grounded circuit capable of delivering at
least 20 A at 230 V
~
.
Table 2-2.
Power plug and receptacle configurations
Location Unit Plug supplied
Receptacle required
(customer supplied)
US/Canada
and Japan
Instrument and
power supply fan
module
NEMA 6-15P
(Quantity 2)
NEMA 6-15R or 6-20R
(Quantity 2)
Computer and
monitor
NEMA 5-15P
(Quantity 2)
NEMA 5-15R or 5-20R
(Quantity 2)
Europe, UK,
and Australia
Instrument,
power supply fan
module, computer,
and monitor
Country-standard 230V
(Quantity 4)
Country-standard 230V
(Quantity 4)
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2.4 Requirements for nitrogen cylinders and pressure regulators
MegaBACE 500 and 1000 Planning Guide ● p2-7
2.3.4 Computer and monitor
The computer and monitor can be connected to any appropriate AC outlet as
long as you make sure the voltage selection switch on the back of the computer
correctly matches the voltage at the outlet (table 2-3). See table 2-2 for plug
and receptacle configurations.
2.4 Requirements for nitrogen cylinders and
pressure regulators
You can use a centralized nitrogen source or provide a separate nitrogen
cylinder for each instrument. This section describes the requirements for
a single locally installed nitrogen
cylinder for both high- and low-pressure
nitrogen, but the principles apply similarly to remote nitrogen sources.
Warning When you install a nitrogen cylinder, make sure you bolt a standard cylinder
bracket to a solid permanent structure in a manner that meets or exceeds
all local seismic and safety code requirements. Failure to secure the nitrogen
cylinder can cause injury to the operator.
2.4.1 Regulator configuration
A single nitrogen source for both high- and low-pressure supplies—
• High pressure for injecting and removing the matrix.
• Low pressure for rinsing the capillaries and operating the cathode and
anode stages and the service door.
Note: If you are using multiple instruments, contact MegaBACE System
Technical Support for the recommended configuration. See Assistance in the
preface for contact information.
Warnings Do not attempt to adjust the regulators to pressure settings above those described
in this guide and in the MegaBACE instrument user’s documentation. If you are
using separate cylinders for high and low pressure, make sure that the correct
pressure is applied to each line.
The nitrogen pressure in the high-pressure line must not exceed 6.89 × 10
3
kPa
(1 000 psi). Never apply high pressure to the low-pressure line. This can damage
the instrument or the low-pressure line and can cause injury.
Use only hose types with ratings that exceed the required operating pressures.
Do not use a frayed or damaged hose, which can rupture and can cause injury.
Table 2-3.
Voltage output by location
Location Volts (AC)
US/Canada 110–120
Japan 100–120
Europe 220–240
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