Ankom 2000 User manual

Operator’s Manual
2052 O’Neil Rd, Macedon NY 14502
Telephone: [phone]
Fax: [phone]
www.ankom.com
Rev 05/12/21

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Table of Contents
Introduction..................................................................................................................................................................5
Warranty.......................................................................................................................................................................5
Filter Bags .....................................................................................................................................................................5
Operating Environment ................................................................................................................................................5
Contact Information .....................................................................................................................................................6
Instrument Description.................................................................................................................................................7
Safety Precautions ......................................................................................................................................................10
Instrument Installation ...............................................................................................................................................11
Fiber Analysis Support Items ......................................................................................................................................12
Analysis Options using the ANKOM2000 Fiber Analyzer...............................................................................................12
ADF Analysis................................................................................................................................................................13
NDF Analysis ...............................................................................................................................................................19
Crude Fiber Analysis ...................................................................................................................................................25
Flush Procedure..........................................................................................................................................................29
Periodic Maintenance.................................................................................................................................................31
Troubleshooting & Replacement Parts.......................................................................................................................35
Appendix A - Analytical Procedures............................................................................................................................36
Appendix B –Wiring Diagrams (1 of 4).......................................................................................................................40

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Operator’s Manual
Rev 05/12/21 pg. 5
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical
laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM
Technology can provide you with products for determining or monitoring detergent fibers, dietary fibers, fat,
digestibility, microbial fermentation (anaerobic or aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of
customer needs.
Congratulations on your purchase of the ANKOM2000 Fiber Analyzer.We are confident that this product will
effectively serve your needs.
By carefully following the operating instructions in this manual, you will minimize errors in results. Experience
indicates that errors in results are usually associated with minor variations in carrying out the procedure. This
manual will provide you with details that will help assure accuracy of your results.
Please review the entire contents of this manual before you begin operating
this instrument.
Warranty
ANKOM Technology warrants the ANKOM2000 Fiber Analyzer against any defects due to faulty workmanship or
material for one year after the original date of purchase. This warranty does not include damage to the instrument
resulting from neglect or misuse. During the warranty period, should any failure result from defects in workmanship
or materials, ANKOM Technology will, at its discretion, repair or replace the instrument free of charge.
Extended warranties are available for purchase if desired.
Filter Bags
ANKOM Technology filter bags (part # F57 and F58) are designed to produce precision and accurate fiber results.
The F57 bags should be used when grinding samples using a cutter type mill with a 1 mm screen which is typically
referenced by the approved methods. For finer ground samples or if particle loss is suspected, use the F58 bags
which have a finer pore size.
Use of other types of filtration media not tested and approved by ANKOM Technology may cause damage to
electrical valves and other components and void your warranty. Filter bags can be purchased from ANKOM
Technology or from your local authorized ANKOM distributor.
Operating Environment
Your ANKOM2000 Fiber Analyzer is designed to operate within the following environments:
•Ambient Temperature Range: 15°−30°C
•Humidity: 20−60% RH
•Power (domestic): 110V−120V ~ 50/60Hz 15A
•Power (international): 220V−240V ~ 50/60Hz 10A

Operator’s Manual
pg. 6 Rev 05/12/21
Contact Information
At ANKOM Technologywe are committed to your total satisfaction and therefore always available to help you get
the most from your ANKOM products. We are also very interested in any comments or suggestions you may have
to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
•Telephone: [phone]
•Fax: [phone]
•Email: [e-mail]
•www.ankom.com

Operator’s Manual
Rev 05/12/21 pg. 7
Instrument Description
General Description
The ANKOM2000 Fiber Analyzer is designed to efficiently and accurately determine Acid Detergent Fiber (ADF),
Neutral Detergent Fiber (NDF), and Crude Fiber within food and/or feed samples. Enabled by Filter Bag
Technology, up to 24 samples can be processed at one time.
During analysis cell contents are removed as the encapsulated sample is subjected to the appropriate chemical (AD,
ND, or crude fiber acid and base) solutions, leaving the desired fiber fraction. Results are determined
gravimetrically. The filter bags are designed to allow proper flow of solutions while retaining non-soluble
components. The fiber residue captured in the filter bag can be used for follow-on assays such as ADIN, NDIN, and
ADL.
Like the ANKOM200 Fiber Analyzer, digestion and rinse operations are all performed within the same instrument,
allowing for the elimination of the separate filtration step. Process temperatures are precisely controlled while
providing proper agitation to ensure a uniform flow of chemical solutions and rinses across each sample.
Below are detailed views of the ANKOM2000 Fiber Analyzer.
Keypad
Display
Vessel Lid
Vessel
Vessel Clamp Handle
Temperature Controller
Power Cord
Power Switch

Operator’s Manual
pg. 8 Rev 05/12/21
Port A –Used for Crude Fiber
Acid solution and ND solution
Vent Tube
Line to Hot Water Supply
Drain Hose
Water Filter
Water Supply Fitting
Left Side View
Right Side View
Power Switch
Port B –Used for Crude Fiber Base solution, AD
solution, and Diluted Amylase
Power Cord Inlet
Temperature Controller
Fuses
Domestic: 15A/120V
International: 10A/220V

Operator’s Manual
Rev 05/12/21 pg. 9
Internal Components
SICK Fiber
Optic
Module - for
Instruments
delivered
before 4/09
(part # A18)
Vent Valve
(part # A22)
24 VDC
Power
Supply
(part # 963)
High Voltage DIN Rail
Drain Valve
Level Sensor
Fitting
(part # A32)
ETS Switch
(part # 622)
Water
Supply Valve
(part # A21)
Maintenance
Alert
Collector
(part # F27)
Motor Coupler
(part # 632)
24V DIN Rail
Motor
(part # A08 –120/60)
(part # A10 –220/50)
Digestion
Vessel
Temperature
Probe
(part # A17)
Analog Level
Sensor
Module - for
Instruments
delivered
after 4/09
(part # A29)
A2000 Computer
(part # A09)
Check Valve
Assembly
Pressure Transducer (part # 6121)
&
Fitting Assembly (part # A25)
Valve B
(part # A19 B)
Valve A
(part # A19 A)

Operator’s Manual
pg. 10 Rev 05/12/21
Safety Precautions
Hazardous Pressure –Do NOT open the Vessel Lid during operation. The
contents of the Vessel are hot and under pressure. Failure to observe this
caution may result in scalding or burning.
Hot Surfaces –Do NOT touch the Vessel surfaces during operation. The
surface can exceed 70°C (158°F). Failure to observe this caution may
result in burning.
Hazardous Voltages –Do NOT operate the instrument with the cover
removed. Hazardous voltages are present during operation. The Power Cord
must be disconnected prior to removal of the rear panel. Failure to observe
this caution may result in electrical shock or electrocution.
Hazardous Materials –Caution should be used when handling hot effluent
that may be caustic or corrosive. If necessary, the solution can be collected in
a container and neutralized before disposal. Follow safe laboratory practices
according to your local regulations when installing and using this instrument
and associated chemicals.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may
result in serious injury or even death.
This system is designed to meet and/or exceed the applicable
standards of CE, CSA, NRTL and OSHA.
•The Power Switch must be in the OFF position before plugging the
instrument Power Cord into the power source.
•In the event of an instrument malfunction, the internal heater will be
automatically turned off by one of the following safety devices:
1) Electrical Fuses
2) The Emergency Temperature Shut-off Switch (ETS)
3) The Pressure Transducer
•Do NOT open the Vessel Lid during or after an operation until both
pressure and liquid are thoroughly exhausted. Connect and secure the
Drain Hose along the path to the drain so it will not move when hot
pressurized fluid is exhausted. Failure to secure the hose could result in
uncontrolled chemical flow.
Please review the entire contents of this manual before you begin operating
this instrument.

Operator’s Manual
Rev 05/12/21 pg. 11
Instrument Installation
Site Requirements
To install and operate the ANKOM2000 Fiber Analyzer you will need the following:
•Adjustable wrench
•Water supply located within 6 feet (3 meters) of the ANKOM2000 and capable of heating water to 50°C for
Crude Fiber and 70°C for ADF/NDF analyses
•Adequate power (see “Operating Environment” section)
•Drain
Instrument Installation Procedure
To install the ANKOM2000 Fiber Analyzer, follow the procedure detailed below.
1.
Remove the instrument from the shipping container and place it in an area that is within 6 feet of a drain
and water supply on a surface that is firm and level. The instrument must not be subject to excessive
shock, vibration, dirt, moisture, oil, or other fluids.
Do NOT place this instrument near microwave ovens or mechanical devices.
Your instrument comes complete with a Power Cord, a Drain Hose, a Vent Tube, a Bag Suspender
Assembly (including Bag Suspender Trays and a Bag Suspender Weight), and an Amylase Container.
2.
With the Power Switch in the OFF position, plug the Power Cord into the Power Cord Inlet on the
instrument.
3.
Plug the Power Cord into the power source.
4.
Install the Water Filter assembly. Attach ¼” copper tubing to the hot water source (+50°C Crude Fiber,
+70°C ND and AD) and to the Water Supply Fitting located on the left side of the instrument.
5.
Connect and secure the Drain Hose so that it will not move when hot pressurized fluid is exhausted.

Operator’s Manual
pg. 12 Rev 05/12/21
6.
If you are using Cubitainers for your chemicals, install the Cubitainer Shelf shown below (Optional).
The solutions are gravity fed into the instrument ports. The bottom of the
solution level must be at least 5” (13cm) above the port. The top of the
solution level can be no more than 20” (50cm) above either port or the
solution will overflow into the drain.
Fiber Analysis Support Items
The following support items are needed to perform the fiber analysis procedures:
Item
Recommended Product
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags
ANKOM #F57, #F58
Bag Holder (used for adding sample to an empty filter bag)
ANKOM #101.2
Heat Sealer for sealing the filter bags
ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker
ANKOM #F08
Desiccant Pouch
ANKOM #X45
Oven for drying (capable of maintaining 102°C ± 2°)
ANKOM #RD (120V), #RDI (220V)
Sample
Spoon
Analysis Options using the ANKOM2000 Fiber Analyzer
The ANKOM2000 Fiber Analyzer can be configured to run ADF, NDF, and Crude Fiber analyses. The instrument
will run with default digestion and rinse time settings unless you select the Custom analysis option. This option
allows you to do ADF, NDF, or Crude Fiber analyses using custom digestion and rinse time settings.
For maintenance purposes, the ANKOM2000 also provides the capability to flush the solution lines.
The following sections provide the information you will need to use and maintain the ANKOM2000 Fiber Analyzer.

Operator’s Manual
Rev 05/12/21 pg. 13
ADF Analysis
ADF Calculation
ADF contained within a food or feed sample can be calculated using the following formula:
% ADF (as-received basis)
=
100 x (W3–(W1 x C1))
W2
Where:
W1
=
Bag tare weight
W2
=
Sample weight
W3
=
Dried weight of filter bag with fiber after extraction process
C1
=
Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
ADF Sample Preparation Procedure
To prepare samples for fiber analysis, follow the procedure detailed below.
When using the ANKOM2000 Fiber Analyzer for ADF analysis, at least one
blank filter bag should be included with the sample set as an indicator of
particle loss. A running average of the blank bag weights is used in the fiber
calculation as the C1 correction factor. A C1value larger than 1.0000 indicates
that sample particles were lost from filter bags and deposited on the blank
bag. Any fiber particle loss from the filter bags will generate erroneous
results. If particle loss is observed, the grinding method for the specific
sample should be evaluated.
1.
Using a Solvent Resistant Marker, number all of the filter bags you will use
during the fiber analysis.
2.
Weigh and record the weight of each empty filter bag (W1).
3.
Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to
sealer.)
4.
Seal at least one empty filter bag (to be used as a blank) within
4mm of its open end. Keep the sealer arm down for 2 –3
seconds after the red sealer light turns off (to cool the seal).
The seal can be seen as a solid melted stripe along the top
edge of the filter bag (as shown to the right). If the seal is not
strong, re-seal the bag.
5.
Place an empty filter bag in the Bag Holder in an open position.
6.
Tare the weight of the empty filter bag and the holder together.
7.
Add 0.45 –0.50g of sample to the filter bag. Keep all particles away from the
sealing area of the filter bag.
8.
Record the weight of the sample (W2) and tare.
Seal

Operator’s Manual
pg. 14 Rev 05/12/21
9.
Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 –3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
10.
Spread the sample out uniformly within the filter bag by shaking and flicking the bag to eliminate
clumping.
11.
Repeat steps 5 –10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed
during one procedure.)
If your samples contain soybean products or >5% fat:
Before doing the ADF analysis in the ANKOM2000, you will need to do a pre-
extraction. For samples containing non-roasted soybean or >5% fat, follow
the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a
top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean:
Before doing the ADF analysis in the ANKOM2000, you will need to do a pre-
extraction. For samples containing roasted soybean, follow the pre-extraction
steps below:
1. Place the filter bags with sample (up to 23) into a container with a
top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak
for twelve hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
12.
Place the filter bags with sample and at least one empty bag (used as a Blank) into
the Bag Suspender trays as shown (maximum of three bags per tray).
13.
Stack each tray on the Bag Suspender rod (eight trays in total) with each tray
rotated 120 degrees from the tray below.
You must use all eight trays even if they are empty.
14.
Add the 9th tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a cover.
The samples are now ready for the ADF analysis procedure.

Operator’s Manual
Rev 05/12/21 pg. 15
ADF Analysis Procedure using the ANKOM2000 Fiber Analyzer
If you are following the acid detergent procedure with an acid detergent lignin,
you can find the lignin procedure on our website at www.ankom.com.
To perform ADF analysis on prepared samples, follow the procedure detailed below.
If you are changing from one analysis type to another, flush the system before
running. See the “Flush Procedure” section of this manual for more
information.
1.
Verify that the hot water supply is on and the drain hose is securely positioned in the drain.
2.
If you are using Cubitainers for your chemicals, attach the AD solution
hose first to the Cubitainer and then to Port B on the instrument.
The solutions are gravity fed into the instrument ports. The bottom of the
solution level must be at least 5” (13cm) above the port. The top of the
solution level can be no more than 20” (50cm) above either port or the
solution will overflow into the drain.
3.
Open the Vessel Lid.
4.
Place the Bag Suspender with the samples into the Vessel.
5.
Place the Bag Suspender Weight onto the Bag Suspender rod to hold the trays in place.
6.
Turn the instrument Power Switch to the ON position. The Display will light and allow you to select an
analysis procedure.
7.
Press the arrow keys on the Keypad until you see “Select ADF” on the
Display.
The ADF analysis will use the following default settings:
•60 minute digestion
•four 5 minute rinses
If you want to run a custom ADF analysis that allows you to set the digestion
time and the number of rinses, press the arrow keys until you see “Select
Custom”on the Display.
Select ADF
v<Enter>

Operator’s Manual
pg. 16 Rev 05/12/21
8.
Press ENTER on the Keypad and follow the prompts on the Display to set up the instrument for ADF
analysis.
9.
Close the Vessel Lid and tighten it by turning the Vessel Clamp
Handle.
If you do not want to use the Cubitainers to automatically add your solutions,
you can manually fill the vessel for each procedure. First make sure that the
hoses to the Cubitainers are disconnected. Immediately after executing step
10 below, pour 2L of solution directly into the vessel. Please note that the
solution must cover the level sensor in order for the instrument to start its
operation. Once agitation begins, close the Vessel Lid and continue the
procedure with step 11.
10.
Press START on the Keypad. Solution from the Cubitainer will flow into the vessel through Port B.
Once the analysis begins, digestions and rinses occur automatically, with the
analyzer Display providing information about the process time remaining, the
temperature, and the pressure. Pressing STOP on the Keypad at any time
during the analysis ends the operation and opens the drain to exhaust the
solution.
11.
When the “Extraction Done” message appears on the Display, the analyzer operation is complete. Open
the Vessel Lid, remove the bags and place them in an appropriately sized beaker.
12.
With your hands, gently press out excess water from the bags into the
beaker and pour off the water from the beaker.
13.
With the bags in the beaker, add enough acetone to cover them. Let the
bags soak in acetone for 3 –5 minutes. Then pour off the acetone.
14.
With your hands, gently press out excess acetone from the bags into the
beaker and pour off the acetone from the beaker.
15.
Remove the bags from the beaker and place them on a wire screen to air-dry.
Hazardous Materials –Do NOT place bags in an oven until all acetone has
evaporated.
16.
Place air-dried bags in the oven and heat at 102°C ± 2° for 2 –4 hours (depending on the oven).
When running a lignin procedure or a sequential (NDF/ADF or
NDF/ADF/Lignin) with F57 Filter Bags it is important not to dry the bags
overnight after the NDF or ADF procedure. A drying timeframe of 2-4 hours
at 100°C to 105°C is sufficient to thoroughly dry the bags after each
procedure. Extended drying times or too high a temperature can compromise
the bag’s filtration media. In addition, after the fourth rinse during the ADF
procedure if you would like to confirm the acid has been completely rinsed
from the bags, capture some water from the waste tube and check the water
with litmus paper.

Operator’s Manual
Rev 05/12/21 pg. 17
17.
Remove the bags from the oven and place them in a Desiccant Pouch.
Do NOT use conventional countertop or cabinet desiccators for this analysis.
18.
Allow the bags to cool to room temperature. This should take about 10 –15 minutes.
19.
Remove one filter bag from the Desiccant Pouch. Press the pouch to remove ambient air and zip it tight.
20.
Re-weigh the filter bag (W3) immediately.
21.
Repeat steps 19 and 20 for each filter bag in the Desiccant Pouch.
22.
Calculate ADF using the following formula.
% ADF (as-received basis)
=
100 x (W3–(W1 x C1))
W2
Where:
W1
=
Bag tare weight
W2
=
Sample weight
W3
=
Dried weight of filter bag with fiber after extraction process
C1
=
Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
If you are following the acid detergent procedure with an acid detergent
lignin, you can find the lignin procedure on our website at www.ankom.com.

Operator’s Manual
pg. 18 Rev 05/12/21
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Operator’s Manual
Rev 05/12/21 pg. 19
NDF Analysis
NDF Calculation
NDF contained within a food or feed sample can be calculated using the following formula:
% NDF (as-received basis)
=
100 x (W3–(W1 x C1))
W2
Where:
W1
=
Bag tare weight
W2
=
Sample weight
W3
=
Dried weight of filter bag with fiber after extraction process
C1
=
Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
NDF Sample Preparation Procedure
To prepare samples for fiber analysis, follow the procedure detailed below.
When using the ANKOM2000 Fiber Analyzer for NDF analysis, at least one
blank filter bag should be included with the sample set as an indicator of
particle loss. A running average of the blank bag weights is used in the fiber
calculation as the C1 correction factor. A C1value larger than 1.0000 indicates
that sample particles were lost from filter bags and deposited on the blank
bag. Any fiber particle loss from the filter bags will generate erroneous
results. If particle loss is observed, the grinding method for the specific
sample should be evaluated.
1.
Using a Solvent Resistant Marker, number all of the filter bags you will use
during the fiber analysis.
2.
Weigh and record the weight of each empty filter bag (W1).
3.
Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to
sealer.)
4.
Seal at least one empty filter bag (to be used as a blank) within
4mm of its open end. Keep the sealer arm down for 2 –3
seconds after the red sealer light turns off (to cool the seal).
The seal can be seen as a solid melted stripe along the top
edge of the filter bag (as shown to the right). If the seal is not
strong, re-seal the bag.
5.
Place an empty filter bag in the Bag Holder in an open position.
6.
Tare the weight of the empty filter bag and the holder together.
7.
Add 0.45 –0.50g of sample to the filter bag. Keep all particles away from the
sealing area of the filter bag.
8.
Record the weight of the sample (W2) and tare.
Seal

Operator’s Manual
pg. 20 Rev 05/12/21
9.
Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 –3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
10.
Spread the sample out uniformly within the filter bag by shaking and flicking the bag to eliminate
clumping.
11.
Repeat steps 5 –10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed
during one procedure.)
If your samples contain soybean products or >5% fat:
Before doing the NDF analysis in the ANKOM2000, you will need to do a pre-
extraction. For samples containing non-roasted soybean or >5% fat, follow
the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a
top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean:
Before doing the NDF analysis in the ANKOM2000, you will need to do a pre-
extraction. For samples containing roasted soybean, follow the pre-extraction
steps below:
1. Place the filter bags with sample (up to 23) into a container with a
top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak
for twelve hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
12.
Place the filter bags with sample and at least one empty bag (used as a Blank) into
the Bag Suspender trays as shown (maximum of three bags per tray).
13.
Stack each tray on the Bag Suspender rod (eight trays in total) with each tray
rotated 120 degrees from the tray below.
You must use all eight trays even if they are empty.
14.
Add the 9th tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a cover.
The samples are now ready for the NDF analysis procedure.