Ankom Delta User manual

Operator’s Manual
2052 O’Neil Rd, Macedon NY 14502
Telephone: [phone]
Fax: [phone]
www.ankom.com
Rev 4/24/20

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Table of Contents
Introduction..................................................................................................................................................................5
Warranty.......................................................................................................................................................................5
Filter Bags .....................................................................................................................................................................5
Operating Environment ................................................................................................................................................5
Contact Information .....................................................................................................................................................6
Instrument Description.................................................................................................................................................7
Safety Precautions ......................................................................................................................................................10
Instrument Installation ...............................................................................................................................................11
Fiber Analysis Support Items ......................................................................................................................................12
Analysis Options using the ANKOMDELTA Automated Fiber Analyzer..........................................................................12
ADF Analysis................................................................................................................................................................13
NDF Analysis ...............................................................................................................................................................19
Crude Fiber Analysis ...................................................................................................................................................25
Flush Procedure..........................................................................................................................................................29
Periodic Maintenance.................................................................................................................................................31
Troubleshooting & Replacement Parts.......................................................................................................................36

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Operator’s Manual
Rev 4/24/20 pg. 5
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical
laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM Technology
can provide you with products for determining or monitoring detergent fibers, dietary fibers, fat, digestibility,
microbial fermentation (anaerobic or aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of
customer needs.
Congratulations on your purchase of the ANKOMDELTA Automated Fiber Analyzer.We are confident that this product
will effectively serve your needs.
By carefully following the operating instructions in this manual, you will minimize errors in results. Experience
indicates that errors in results are usually associated with minor variations in carrying out the procedure. This manual
will provide you with details that will help assure accuracy of your results.
NOTE:
Please review the entire contents of this manual before you begin operatingthis
instrument.
Warranty
Unless otherwise stated or agreed upon, ANKOM Technology warrants the ANKOMDELTA Automated Fiber Analyzer
against any defects due to faulty workmanship or material for one year after the original date of purchase. This
warranty does not include damage to the instrument resulting from neglect or misuse. During the warranty period,
should any failure result from defects in workmanship or materials, ANKOM Technology will, at its discretion, repair
or replace the instrument free of charge.
Extended warranties are available for purchase if desired.
Filter Bags
ANKOM Technology Filter Bags (part # F57 and F58) are designed to produce precision and accurate fiber results.
The F57 bags should be used when grinding samples using a cutter type mill with a 1 mm screen which is typically
referenced by the approved methods. For finer ground samples or if particle loss is suspected, use the F58 bags which
have a finer pore size.
Use of other types of filtration media not tested and approved by ANKOM Technology may cause damage to electrical
valves and other components and void your warranty. Filter bags can be purchased from ANKOM Technology or
from your local authorized ANKOM distributor.
Operating Environment
Your ANKOMDELTA Automated Fiber Analyzer is designed to operate within the following environments:
•Ambient Temperature Range: 15°−30°C
•Humidity: 20−60% RH
•Power (domestic): 110V−120V ~ 50/60Hz 15A
•Power (international): 220V−240V ~ 50/60Hz 10A

Operator’s Manual
pg. 6 Rev 4/24/20
Contact Information
At ANKOM Technologywe are committed to your total satisfaction and therefore always available to help you get
the most from your ANKOM products. We are also very interested in any comments or suggestions you may have to
help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
•Telephone: [phone]
•Fax: [phone]
•Email: [e-mail]
•www.ankom.com

Operator’s Manual
Rev 4/24/20 pg. 7
Instrument Description
General Description
The ANKOMDELTA Automated Fiber Analyzer is designed to efficiently and accurately determine Acid Detergent
Fiber (ADF), Neutral Detergent Fiber (NDF), and Crude Fiber within food and/or feed samples. Enabled by Filter
Bag Technology, up to 24 samples can be processed at one time.
During analysis cell contents are removed as the encapsulated sample is subjected to the appropriate chemical (AD,
ND, or crude fiber acid and base) solutions, leaving the desired fiber fraction. Results are determined gravimetrically.
The filter bags are designed to allow proper flow of solutions while retaining non-soluble components. The fiber
residue captured in the filter bag can be used for follow-on assays such as ADIN, NDIN, and ADL.
Like the ANKOM200 and ANKOM2000 Fiber Analyzers, digestion and rinse operations are all performed within the
same instrument, allowing for the elimination of the separate filtration step. Process temperatures are precisely
controlled while providing proper agitation to ensure a uniform flow of chemical solutions and rinses across each
sample. The ANKOMDELTA Automated Fiber Analyzer contains a series of pumps that automatically pull the
appropriate chemical into the instrument from a chemical container, i.e. cubitainer. No gravity feed is necessary.
Below are detailed views of the ANKOMDELTA Automated Fiber Analyzer.
Keypad
Display
Vessel Lid
Vessel
Vessel Clamp Handle
Temperature Controller
Power Cord Inlet
Power Switch

Operator’s Manual
pg. 8 Rev 4/24/20
Amylase port (Used during ND analysis)
Vent Tube
Neutral Detergent
Drain Hose
Acid Detergent
Water
Left Side View
Right Side View
Power Switch
Power Cord Inlet
Temperature Controller
Fuses
Domestic: 15A/120V
International: 10A/220V
Crude Fiber Acid
Crude Fiber Base
Window

Operator’s Manual
Rev 4/24/20 pg. 9
Internal Components
Vent Valve
(part # A22)
24 VDC
Power
Supply
(part # 963)
High Voltage DIN Rail
Drain Valve
Level Sensor
Fitting
(part # A32)
Pump
(part # 6178)
Water
Supply Valve
(part # A21)
Maintenance
Alert
Collector
(part # F27)
Motor Coupler
(part # 632)
Motor
(part # A08 –120/60)
(part # A10 –220/50)
Temperature
Probe
(part # A17)
LCD Screen
(part # X90)
I/O Board
(part# 8300)
Pressure Transducer (part # 6121)
&
Fitting Assembly (part # A25)
Splat Controller
(part # A09)
Spice
(part # 6104)
Valves
(part # 6145)
Level
Sensor
Module
(part # A29)

Operator’s Manual
pg. 10 Rev 4/24/20
Safety Precautions
Hazardous Pressure –Do NOT open the Vessel Lid during operation. The
contents of the Vessel are hot and under pressure. Failure to observe this
caution may result in scalding or burning.
Hot Surfaces –Do NOT touch the Vessel surfaces during operation. The
surface can exceed 70°C (158°F). Failure to observe this caution may result
in burning.
Hazardous Voltages –Do NOT operate the instrument with the cover
removed. Hazardous voltages are present during operation. The Power Cord
must be disconnected prior to removal of the rear panel. Failure to observe
this caution may result in electrical shock or electrocution.
Hazardous Materials –Caution should be used when handling hot effluent
that may be caustic or corrosive. If necessary, the solution can be collected in
a container and neutralized before disposal. Follow safe laboratory practices
according to your local regulations when installing and using this instrument
and associated chemicals.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may
result in serious injury or even death.
This system is designed to meet and/or exceed the applicable
standards of CE, CSA, NRTL and OSHA.
IMPORTANT:
•The Power Switch must be in the OFF position before plugging the
instrument Power Cord into the power source.
•In the event of an instrument malfunction, the internal heater will be
automatically turned off by one of the following safety devices:
1) Electrical Fuses
2) The Emergency Temperature Shut-off Switch (ETS)
3) The Pressure Transducer
•Do NOT open the Vessel Lid during or after an operation until both pressure
and liquid are thoroughly exhausted. Connect and secure the Drain Hose
along the path to the drain so it will not move when hot pressurized fluid is
exhausted. Failure to secure the hose could result in uncontrolled
chemical flow.
NOTE:
Please review the entire contents of this manual before you begin operatingthis
instrument.

Operator’s Manual
Rev 4/24/20 pg. 11
Instrument Installation
Site Requirements
To install and operate the ANKOMDELTA Automated Fiber Analyzer you will need the following:
•Adjustable wrench
•Water supply located within 6 feet (3 meters) of the ANKOMDELTA regulated to 10-20 psi or less, and
capable of heating water to 50°C for Crude Fiber and 70°C for ADF/NDF analyses
•Adequate power (see “Operating Environment” section)
•Drain
Instrument Installation Procedure
To install the ANKOMDELTA Automated Fiber Analyzer, follow the procedure detailed below.
1.
Remove the instrument from the shipping container and place it in an area that is within 6 feet of a drain
and water supply on a surface that is firm and level. The instrument must not be subject to excessive
shock, vibration, dirt, moisture, oil, or other fluids.
IMPORTANT:
Do NOT place this instrument near microwave ovens or mechanical
devices.
Your instrument comes complete with a Power Cord, a Drain Hose, a Vent Tube, a Bag Suspender
Assembly (including Bag Suspender Trays and a Bag Suspender Weight), an Amylase Container, and,
unless otherwise agreed upon, four six foot chemical tubes for the acid detergent, neutral detergent, crude
fiber acid and crude fiber base valves.
2.
With the Power Switch in the OFF position, plug the Power Cord into the Power Cord Inlet on the
instrument.
3.
Plug the Power Cord into the power source.
4.
Install the Water Regulator Filter assembly. Attach ¼” copper tubing to the hot water source (+50°C
Crude Fiber, +70°CND and AD) and to the Water Supply Fitting located on the leftside of the instrument.
5.
Connect and secure the Drain Hose so that it will not move when hot pressurized fluid is exhausted.
6.
Connect the four chemical tubes to their appropriate valves. Place the other end securely in your
chemical container. If you are using cubitainers for your chemicals, they can be placed anywhere within
six feet of the instrument. If you are using another form of chemical container (i.e. large tanks) they may
be placed anywhere within six feet of the instrument. The system will pump the chemical into the
instrument, it is not gravity fed.

Operator’s Manual
pg. 12 Rev 4/24/20
Fiber Analysis Support Items
The following support items are needed to perform the fiber analysis procedures:
Item
Recommended Product
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags
ANKOM #F57, #F58
Bag Holder (used for adding sample to an empty filter bag)
ANKOM #101.2
Heat Sealer for sealing the filter bags
ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker
ANKOM #F08
Desiccant Pouch
ANKOM #X45
Oven for drying (capable of maintaining 102°C ± 2°)
ANKOM #RD (120V), #RDI (220V)
Sample
Spoon
Hot Water Heater
ANKOM A02
Water Filter Regulator Assembly
Acid Detergent
ANKOM FAD20CB cubitainer
ANKOM FAD20 Concentrate
ANKOM FAD20C, Concentrate-dry CTAB powder
Neutral Detergent
ANKOM FND20 cubitainer
ANKOM FND20C Dry Concentrate
Crude Fiber Acid
ANKOM FCFA20CB cubitainer
ANKOM FCFA20 Concentrate
Crude Fiber Base
ANKOM FCFB20CB cubitainer
ANKOM FCFB20 Concentrate
Alpha Amylase
ANKOM FAA
Analysis Options using the ANKOMDELTA Automated Fiber Analyzer
The ANKOMDELTA Automated Fiber Analyzer can be configured to run ADF, NDF, and Crude Fiber analyses. The
instrument will run with default digestion and rinse time settings unless you select the Custom analysis option. This
option allows you to do ADF, NDF, or Crude Fiber analyses using custom digestion and rinse time settings.
For maintenance purposes, the ANKOMDELTA also flushes the chemical lines and valves after each run automatically.
The following sections provide the information you will need to use and maintain the ANKOMDELTA Automated Fiber
Analyzer.

Operator’s Manual
Rev 4/24/20 pg. 13
ADF Analysis
ADF Calculation
ADF contained within a food or feed sample can be calculated using the following formula:
% ADF
=
100 x (W3–(W1 x C1))
(as-received basis)
W2
Where:
W1
=
Bag tare weight
W2
=
Sample weight
W3
=
Dried weight of filter bag with fiber after extraction process
C1
=
Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
ADF Sample Preparation Procedure
To prepare samples for fiber analysis, follow the procedure detailed below.
IMPORTANT:
When using the ANKOMDELTA Automated Fiber Analyzer for ADF analysis, at
least one blank filter bag should be included with the sample set as an indicator
of particle loss. A running average of the blank bag weights is used in the fiber
calculation as the C1 correction factor. A C1value larger than 1.0000 indicates
that sample particles were lost from filter bags and deposited on the blank bag.
Any fiber particle loss from the filter bags will generate erroneous results. If
particle loss is observed, the grinding method for the specific sample should be
evaluated.
1.
Using a Solvent Resistant Marker, number all of the filter bags you will use during
the fiber analysis.
2.
Weigh and record the weight of each empty filter bag (W1).
3.
Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to
sealer.)
4.
Seal at least one empty filter bag (to be used as a blank) within
4mm of its open end. Keep the sealer arm down for 2 –3
seconds after the red sealer light turns off (to cool the seal). The
seal can be seen as a solid melted stripe along the top edge of
the filter bag (as shown to the right). If the seal is not strong, re-
seal the bag.
5.
Place an empty filter bag in the Bag Weigh Holder in an open position.
6.
Tare the weight of the empty filter bag and the holder together.
7.
Add 0.45 –0.50g of sample to the filter bag. Keep all particles away from the sealing
area of the filter bag.
8.
Record the weight of the sample (W2).
Seal

Operator’s Manual
pg. 14 Rev 4/24/20
9.
Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 –3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
10.
Spread the sample out uniformly within the filter bag by shaking and flicking the bag to eliminate
clumping.
11.
Repeat steps 5 –10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed
during one procedure.)
IMPORTANT:
If your samples contain >5% fat:
Before doing the ADF analysis in the ANKOMDELTA, you will need to do a pre-
extraction. For samples containing non-roasted soybean or >5% fat, follow the
pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean:
Before doing the ADF analysis in the ANKOMDELTA, you will need to do a pre-
extraction. For samples containing roasted soybean, follow the pre-extraction
steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak for
twelve hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
12.
Place the filter bags with sample and at least one empty bag (used as a Blank) into
the Bag Suspender trays as shown (maximum of three bags per tray).
13.
Stack each tray on the BagSuspender rod (eight trays in total) with each tray rotated
120 degrees from the tray below.
IMPORTANT:
You must use all eight trays even if they are empty.
14.
Add the 9th tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a cover.
NOTE:
The samples are now ready for the ADF analysis procedure.

Operator’s Manual
Rev 4/24/20 pg. 15
ADF Analysis Procedure using the ANKOMDELTA Automated Fiber Analyzer
NOTE:
If you are following the acid detergent procedure with an acid detergent
lignin, you can find the lignin procedure on our website at www.ankom.com.
To perform ADF analysis on prepared samples, follow the procedure detailed below.
NOTE:
The instrument automatically flushes the chemical lines and valves after
each run.
1.
Verify that the hot water supply is on and the drain hose is securely positioned in the drain.
2.
Ensure the AD chemical tube is securely connected to the acid detergent valve and to your AD solution
container (cubitainer, tank, etc.)
NOTE:
The chemicals are pumped into the instrument via the pump and valves.
3.
Open the Vessel Lid.
4.
Place the Bag Suspender with the samples into the Vessel.
5.
Place the Bag Suspender Weight onto the Bag Suspender rod to hold the trays in place.
6.
Turn the instrument Power Switch to the ON position. The Display will light and allow you to select an
analysis procedure.
7.
Press the arrow keys on the Keypad until you see “Select ADF” on
the Display.
IMPORTANT:
The ADF analysis will use the following default settings:
•60 minute digestion
•four 5 minute rinses
8.
Press ENTER on the Keypad and follow the prompts on the Display to set up the instrument for ADF
analysis.
9.
Close the Vessel Lid and tighten it by turning the Vessel Clamp Handle.
NOTE:
There are many ways you can supply solutions to yourinstrument. The flexible
chemical tubes that attach to each valve allow you to pull solution from any
type of chemical container: cubitainer, large chemical tanks, etc. If you do not
want the instrument to automatically add your solutions, you can manually fill
the vessel for each procedure. First make sure that the tubes to the chemical
containers are disconnected. Immediately after executing step 10 below, pour
2L of solution directly into the vessel. Please note that the solution must cover
the level sensor in order for the instrument tostart its operation. Once agitation
begins, close the Vessel Lid and continue the procedure with step 11.
Select ADF
v<Enter>

Operator’s Manual
pg. 16 Rev 4/24/20
10.
Press START on the Keypad. Solution will flow into the vessel through the acid detergent chemical tube
and valve.
NOTE:
Once the analysis begins, digestions and rinses occur automatically, with the
analyzer Display providing information about the process time remaining, the
temperature, andthe pressure. Pressing STOP on the Keypadat any time during
the analysis ends the operation and opens the drain to exhaust the solution.
11.
When the “Extraction Done” message appears on the Display, the analyzer operation is complete. Open
the Vessel Lid, remove the bags and place them in an appropriately sized beaker.
12.
With your hands, gently press out excess water from the bags into the
beaker and pour off the water from the beaker.
13.
With the bags in the beaker, add enough acetone to cover them. Let the bags
soak in acetone for 3 –5 minutes. Then pour off the acetone.
14.
With your hands, gently press out excess acetone from the bags into the
beaker and pour off the acetone from the beaker.
15.
Remove the bags from the beaker and place them on a wire screen to air-dry.
Hazardous Materials –Do NOT place bags in an oven until all acetone has
evaporated.
16.
Place air-dried bags in the oven and heat at 102°C ± 2° for 2 –4 hours (depending on the oven).
IMPORTANT:
When running a lignin procedure or a sequential (NDF/ADF or
NDF/ADF/Lignin) with F57 Filter Bags it is important not to dry the bags
overnight after the NDF or ADF procedure. A drying timeframe of 2-4 hours
at 100°C to 105°C is sufficient to thoroughly dry the bags after each procedure.
Extended drying times or too high a temperature can compromise the bag’s
filtration media. In addition, be sure to check the water of the fourth rinse
during the ADF procedure to ensure all the sulfuric acid has been removed
from the bags. If litmus paper shows the presence of acid during the fourth hot
water rinse, repeat until neutral.
17.
Remove the bags from the oven and place them in a Desiccant Pouch.
IMPORTANT:
Do NOT use conventional countertop or cabinet desiccators for this analysis.
18.
Allow the bags to cool to room temperature. This should take about 10 –15 minutes.
19.
Remove one filter bag from the Desiccant Pouch. Press the pouch to remove ambient air and zip it tight.
20.
Re-weigh the filter bag (W3) immediately.
21.
Repeat steps 19 and 20 for each filter bag in the Desiccant Pouch.

Operator’s Manual
Rev 4/24/20 pg. 17
22.
Calculate ADF using the following formula.
% ADF
=
100 x (W3–(W1 x C1))
(as-received basis)
W2
Where:
W1
=
Bag tare weight
W2
=
Sample weight
W3
=
Dried weight of filter bag with fiber after extraction process
C1
=
Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
NOTE:
If you are following the acid detergent procedure with an acid detergent
lignin, you can find the lignin procedure on our website at www.ankom.com.

Operator’s Manual
pg. 18 Rev 4/24/20
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Operator’s Manual
Rev 4/24/20 pg. 19
NDF Analysis
NDF Calculation
NDF contained within a food or feed sample can be calculated using the following formula:
% NDF
=
100 x (W3–(W1 x C1))
(as-received basis)
W2
Where:
W1
=
Bag tare weight
W2
=
Sample weight
W3
=
Dried weight of filter bag with fiber after extraction process
C1
=
Blank bag correction (running average of final oven-dried weight divided
by original blank bag weight)
NDF Sample Preparation Procedure
To prepare samples for fiber analysis, follow the procedure detailed below.
IMPORTANT:
When using the ANKOMDELTA Automated Fiber Analyzer for NDF analysis, at
least one blank filter bag should be included with the sample set as an indicator
of particle loss. A running average of the blank bag weights is used in the fiber
calculation as the C1 correction factor. A C1value larger than 1.0000 indicates
that sample particles were lost from filter bags and deposited on the blank bag.
Any fiber particle loss from the filter bags will generate erroneous results. If
particle loss is observed, the grinding method for the specific sample should be
evaluated.
1.
Using a Solvent Resistant Marker, number all of the filter bags you will use during
the fiber analysis.
2.
Weigh and record the weight of each empty filter bag (W1).
3.
Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to
sealer.)
4.
Seal at least one empty filter bag (to be used as a blank) within
4mm of its open end. Keep the sealer arm down for 2 –3
seconds after the red sealer light turns off (to cool the seal). The
seal can be seen as a solid melted stripe along the top edge of
the filter bag (as shown to the right). If the seal is not strong, re-
seal the bag.
5.
Place an empty filter bag in the Bag Weigh Holder in an open position.
6.
Tare the weight of the empty filter bag and the holder together.
7.
Add 0.45 –0.50g of sample to the filter bag. Keep all particles away from the sealing
area of the filter bag.
8.
Record the weight of the sample (W2).
Seal

Operator’s Manual
pg. 20 Rev 4/24/20
9.
Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 –3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
10.
Spread the sample out uniformly within the filter bag by shaking and flicking the bag to eliminate
clumping.
11.
Repeat steps 5 –10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed
during one procedure.)
IMPORTANT:
If your samples contain >5% fat:
Before doing the NDF analysis in the ANKOMDELTA, you will need to do a pre-
extraction. For samples containing non-roasted soybean or >5% fat, follow the
pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean:
Before doing the NDF analysis in the ANKOMDELTA, you will need to do a pre-
extraction. For samples containing roasted soybean, follow the pre-extraction
steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak for
twelve hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
12.
Place the filter bags with sample and at least one empty bag (used as a Blank) into
the Bag Suspender trays as shown (maximum of three bags per tray).
13.
Stack each tray on the BagSuspender rod (eight trays in total) with each tray rotated
120 degrees from the tray below.
IMPORTANT:
You must use all eight trays even if they are empty.
14.
Add the 9th tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a cover.
NOTE:
The samples are now ready for the NDF analysis procedure.