Ankom TDF User manual

Rev 9/20/18
Operator’s Manual
2052 O’Neil Rd, Macedon NY 14502
Telephone: [phone]
Fax: [phone]
www.ankom.com

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Table of Contents
Introduction ..................................................................................................................................................................5
Warranty.......................................................................................................................................................................5
Filter Bags .....................................................................................................................................................................5
Contact Information .....................................................................................................................................................5
Method Comparison (Manual vs. Automated).............................................................................................................6
Instrument Description.................................................................................................................................................7
Service Access Position...............................................................................................................................................10
Operating Environment ..............................................................................................................................................10
Safety Precautions ......................................................................................................................................................10
Instrument Installation ...............................................................................................................................................11
Dietary Fiber Analysis Support Items..........................................................................................................................16
Analysis Options using the ANKOMTDF Dietary Fiber Analyzer ...................................................................................16
Blanks..........................................................................................................................................................................16
IDF/SDF Analysis .........................................................................................................................................................17
TDF Analysis (AOAC 991.43) .......................................................................................................................................31
TDF Analysis (AOAC 985.29 / 2001.03).......................................................................................................................44
Protein Determination................................................................................................................................................59
Ash Determination......................................................................................................................................................61
Productivity Enhancement .........................................................................................................................................63
Status Screen ..............................................................................................................................................................67
Fault Handling.............................................................................................................................................................71
Diagnostics Mode .......................................................................................................................................................73
Periodic Maintenance.................................................................................................................................................81
Troubleshooting & Replacement Parts.......................................................................................................................82
QC / Calibration Checks ..............................................................................................................................................83
Appendix A –Reagents (AOAC 991.43, 985.29, 2001.03) ..........................................................................................89
Appendix B –Instrument Out-of-use Procedure........................................................................................................91
Appendix C –De-fatting Procedure ............................................................................................................................93
Appendix D –Accessories (sold separately) ...............................................................................................................95

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Operator’s Manual
Rev 9/20/18 pg. 5
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories
around the world in the food, feed, bio-energy, agricultural, and environmental industries. ANKOM Technology can provide
you with products for determining or monitoring dietary fiber, detergent fiber, crude fiber, fat, digestibility, microbial
fermentation (anaerobic or aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of customer needs.
Congratulations on your purchase of the ANKOMTDF Dietary Fiber Analyzer.We are confident that this product will
effectively serve your needs.
The ANKOMTDF Dietary Fiber Analyzer was designed to eliminate most of the manual steps required by the method. This
reduces technician variation, increasing precision. By carefully following the operating instructions in this manual you will
understand the details of sample and filter bag handling as well as instrument control, helping you to achieve the best possible
results.
Please review the entire manual before you begin operating this product.
Warranty
ANKOM Technology warrants the ANKOMTDF Dietary Fiber Analyzer against any defects in workmanship or material for one
year after the original date of purchase. This warranty does not include damage to the instrument resulting from neglect or
misuse. During the warranty period, should any failure result from defects in workmanship or materials, ANKOM Technology
will, at its discretion, repair or replace the instrument free of charge.
Extended warranties are available upon request.
Filter Bags
Use ANKOM Technology filter bags (part #’s DF-I, DF-S, DF-FT) in your ANKOMTDF Dietary Fiber Analyzer. Filter bags can
be purchased from ANKOM Technology or from your local authorized ANKOM distributor.
Contact Information
Committed to your total satisfaction, we are always available to help you get the most from your ANKOM products and to
receive any comments or suggestions you might have to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
•Telephone: [phone]
•Fax: [phone]
•Email: [e-mail]
•www.ankom.com

Operator’s Manual
pg. 6 Rev 9/20/18
Method Comparison (Manual vs. Automated)
AOAC 991.43 Method –IDF/SDF assay without automation
Below is the AOAC 991.43 method flow done manually by a technician using glass crucibles and beakers, vacuum to assist
with filtering, and water baths to control temperature and mixing. DE refers to Diatomaceous Earth.
Prepare for Test
Digest Sample
Isolate IDF Residue
Isolate SDF Residue
1
Prepare crucibles
5
Heat to 95°C
17
Transfer to filter
26
Add 95% EtOH to fitrate
1a
Ash crucibles overnight
6
Add buffer
18
Filter with vacuum assist
27
Precipitate for 60 min
1b
Clean with vacuum
7
Add Amylase
19
Scratch filter bed as
needed
28
Transfer to filter
1c
Soak 1 hr @ room temp
8
Digest at 95°C for 35 min
29
Filter with vacuum assist
1d
Rinse with 3 solutions
9
Scrape beaker walls and
rinse with H2O to move
all residue to bottom
20
Rinse with 70°C H2O
30
Scratch filter bed as
needed
1e
Prepare DE filter bed
21
Rinse with 78% EtOH
1f
Dry @ 130°C
22
Rinse with 95% EtOH
31
Rinse with 78% EtOH
1g
Cool 1 hr and weigh
10
Cool to 60°C
23
Rinse with Acetone
32
Rinse with 95% EtOH
2
Weigh samples
11
Add Protease
24
Dry IDF residue
33
Rinse with Acetone
3
Prepare H2O bath(s)
12
Digest at 60°C for 30 min
25
Weigh IDF residue
34
Dry SDF residue
4
Place samples in beakers
13
Add HCl
35
Weigh SDF residue
14
Add AMG
15
Check/adjust pH
Calculate Results
16
Digest at 60°C for 30 min
36
Correct for Protein
37
Correct for Ash
38
Calculate results using
weights and corrections
AOAC 991.43 Method –IDF/SDF assay with ANKOMTDF Dietary Fiber Analyzer automation
Below is the AOAC 991.43 method flow when using the ANKOMTDF Dietary Fiber Analyzer. Steps highlighted in YELLOW
represent tasks done by a technician. Steps highlighted in GREEN (1a-1g, 9, 19, and 30) are no longer needed because of Filter
Bag Technology. All other steps (including mixing) are done automatically by the ANKOMTDF Dietary Fiber Analyzer under
computer control. DE refers to Diatomaceous Earth.
Prepare for Test
Digest Sample
Isolate IDF Residue
Isolate SDF Residue
5
Heat to 95°C
17
Transfer to filter
26
Add 95% EtOH to fitrate
6
Add buffer
18
Filter with pressure assist
27
Precipitate for 60 min
7
Add Amylase
28
Transfer to filter
8
Digest at 95°C for 35 min
29
Filter with pressure assist
20
Rinse with 70°C H2O
21
Rinse with 78% EtOH
22
Rinse with 95% EtOH
31
Rinse with 78% EtOH
1
Weigh filter bags
10
Cool to 60°C
23
Rinse with Acetone
32
Rinse with 95% EtOH
2
Weigh DE and samples
11
Add Protease
24
Dry IDF residue
33
Rinse with Acetone
3
Install filter bags
12
Digest at 60°C for 30 min
25
Weigh IDF residue
34
Dry SDF residue
4
Add DE & Sample to bags
13
Add HCl
35
Weigh SDF residue
14
Add AMG
15
Check/adjust pH
Calculate Results
16
Digest at 60°C for 30 min
36
Correct for Protein
37
Correct for Ash
38
Calculate results using
weights and corrections
This section is just an overview of how the ANKOMTDF Dietary Fiber Analyzer
executes the AOAC 991.43 method. Please see the “IDF/SDF Analysis” and “TDF
Analysis” sections of this manual for detailed instructions on how to perform
IDF/SDF and TDF assays with the analyzer.

Operator’s Manual
Rev 9/20/18 pg. 7
Instrument Description
The ANKOMTDF Dietary Fiber Analyzer is designed to efficiently, accurately, and precisely recover Insoluble Dietary Fiber
(IDF), Soluble Dietary Fiber (SDF), and Total Dietary Fiber (TDF) residue from food and/or feed samples in accordance with
the AOAC 991.43, AOAC 985.29, AOAC 2001.03, AACC 32-07.01, and NMKL 129, 2003 methods.
Contact ANKOM Technology about an upgrade to Total Integrated Dietary Fiber
(AOAC 2009.01 and 2011.25 methods). See the Diagnostics section of this
document for instructions on how to enable these methods.
Enabled by Filter Bag Technology, the instrument can run six digestion and six precipitation processes at the same time. The
filter bags are designed to capture the appropriate fiber particles while allowing all non-fiber components to pass through. The
instrument will use IDF bags and SDF bags when configured to recover IDF and SDF residue. The instrument will use IDF
Flow-Thru bags and SDF bags when configured to recover TDF residue.
The automated fiber recovery is achieved by first digesting samples within bags using enzyme treatments. Depending on the
filter bag configuration, the instrument then collects the IDF and SDF residue using two separate filters, or collects the TDF
residue using one filter. Once captured in the filters the residue is dried, weighed, and corrected for protein and ash content to
determine the IDF, SDF, and/or TDF values.
Digestion, precipitation, rinse (H2O and EtOH), and filter operations are performed by the instrument, eliminating manual
transfer and filtration steps. An on-board computer precisely controls process temperatures, agitation, and fluid delivery along
with digestion, precipitation, and filtration times.
External Components –Front View
Touch
Screen
Display
Filtrate Cup
(TDF53 - #1 of 6)
Waste Tray
IDF Station (#4 of 6)
SDF Delivery
Nozzle
(#1 of 6)
Clamp Bar A
Clamp Bar B
Control
Buttons
Hook for SDF Bag
(#3 of 6)
IDF Delivery
Nozzle
(#1 of 6)
Control Panel (TDF17)
Clamp Bar D
Clamp Bar C
SDF Station (#6 of 6)
Heating Paddle (#1 of 6)
Mixing Pad
(#2 of 6)
Service Access
Screw #1
Service Access Screw #2
Front Cover
(In UP position)
Pressure Gauge

Operator’s Manual
pg. 8 Rev 9/20/18
External Components –Left Side View
External Components –Right Side View
Control Panel (TDF17)
95% EtOH Container (TDF42)
HCl Container (TDF38)
Enzyme Containers
(TDF35, TDF36, TDF37)
Nitrogen Supply Port
80-100 psi
(5.5-6.9 bar)
Front Cover Arm
Power Switch
Front Cover (TDF56)
78% EtOH Container (TDF41)
Buffer Container (TDF39)
Distilled Water Container (TDF40)
Front Cover Arm
Three Container Holder
Single Container Holder
Pressure Gauge

Operator’s Manual
Rev 9/20/18 pg. 9
Internal Components –Rear View
N2
Check Valve
(1 of 12)
Main Electrical Cabinet
IDF Inlet Valve
Stations 4, 5 & 6
SDF Inlet Valve
Stations 4, 5 & 6
In-Line Heater
Tubing Support Panel
IDF Inlet Valve
Stations 1, 2 & 3
SDF Inlet Valve
Stations 1, 2 & 3
Waste Valves
(1 –6)
Supply Valves
−Water
−Buffer
−78% EtOH
−95% EtOH
Vacuum Sensor
Supply
Manifold
Clamp Bar Cylinder
(1 of 8)
HCl
Supply Valve
Peristaltic
Pump
Pressure
Sensors
Drain Tube
Supply Valves
−Amylase
−Protease
−AMG
Torsion Bar (for
Front Cover)

Operator’s Manual
pg. 10 Rev 9/20/18
Service Access Position
If your instrument must be located in such a way as to restrict access to the back, you can tilt it forward to access the rear of the
instrument by removing the two Service Access Screws on the front of the instrument and gently pulling the top of the Main
Electrical Cabinet forward and down. The instrument will pivot at its center.
Operating Environment
Your ANKOMTDF Dietary Fiber Analyzer is designed to operate within the following environments:
•Ambient Temperature Range: 19°−30°C
•Humidity: 20–60% RH
•Power: 100V−120V ~ 50/60Hz 15A
220V−240V ~ 50/60Hz 8A
If after running this instrument you plan to leave it unused for 1 week or longer, you
must clear out the fluid lines to ensure proper operation. See “Appendix B –
Instrument Out-of-use Procedure” for more information.
Safety Precautions
Hot Surfaces –Do NOT touch the Heating Paddle surfaces during operation. The
surface can exceed 100°C (212°F). Failure to observe this caution may result in
burns.
Hazardous Voltages –Do NOT operate the instrument with the back of the Main
Electrical Cabinet removed. Hazardous voltages are present during operation. Failure
to observe this caution may result in electrical shock or electrocution.
Hazardous Materials –Ethanol is used within this instrument. Follow both local
and federal regulations for vent hood requirements when operating this instrument.
Do NOT heat seal or place filter bags in an oven until all acetone has evaporated.
Failure to observe this caution may be hazardous to your health.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may result in
serious injury or even death.
This system is designed to meet and/or exceed the applicable standards of CE and
CSA.
The Power Switch must be in the OFF position before plugging the instrument Power
Cord into the power source.
Please review the entire manual before you begin operating this instrument.
Service Access
Screw #1
Service Access
Screw #2
Main Electrical
Cabinet