Ankom TDF User manual

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 Operator’sManua
l
RevA4/30/12
2052O’NeilRd,MacedonNY14502
Telephone:(315)986‐8090
Fax:(315)986‐8091
www.ankom.com

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TableofContents

Introduction..................................................................................................................................................................5
Warranty.......................................................................................................................................................................5
FilterBags.....................................................................................................................................................................5
OperatingEnvironment................................................................................................................................................5
ContactInformation.....................................................................................................................................................6
InstrumentDescription.................................................................................................................................................6
SafetyPrecautions......................................................................................................................................................10
InstrumentInstallation...............................................................................................................................................11
DietaryFiberAnalysisSupportItems..........................................................................................................................15
AnalysisOptionsusingtheANKOMTDFDietaryFiberAnalyzer...................................................................................15
IDF/SDFAnalysis.........................................................................................................................................................17
TDFAnalysis................................................................................................................................................................30
ProteinDetermination................................................................................................................................................43
AshDetermination......................................................................................................................................................46
Enhanceyourproductivity..........................................................................................................................................49
UnderstandtheStatusScreen....................................................................................................................................53
FaultHandling.............................................................................................................................................................56
DiagnosticsMode.......................................................................................................................................................59
PeriodicMaintenance.................................................................................................................................................71
Troubleshooting..........................................................................................................................................................71
AppendixA–Reagents(AOAC991.43,AACC32‐07.01,NMKL129,2003)................................................................72
AppendixB–StorageProcedure................................................................................................................................74
AppendixC–Parts&Assemblies...............................................................................................................................75
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Operator’sManual
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RevA4/30/12   pg.5
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Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories
around the world in the environmental, agricultural, biomass, and food industries. ANKOM Technology can provide you with
products for determining or monitoring dietary fiber, detergent fiber, fat, digestibility, microbial fermentation (anaerobic or
aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of customer needs.
Congratulations on your purchase of theANKOMTDF Dietary Fiber Analyzer.We are confident that this product will
effectively serve your needs.
The ANKOMTDF Dietary Fiber Analyzer was designed to eliminate most of the manual steps required by the method. This
reduces technician variation, increasing precision. By carefully following the operating instructions in this manual you will
understand the details of sample and filter bag handling as well as the instrument controls, helping you to achieve the best
possible results.
Please review the entire manual before you begin operating this product.
Warranty
ANKOM Technology warrants the ANKOMTDF Dietary Fiber Analyzer against any defects in workmanship or material for one
year after the original date of purchase. This warranty does not include damage to the instrument resulting from neglect or
misuse. During the warranty period, should any failure result from defects in workmanship or materials ANKOM Technology
will, at its discretion, repair or replace the instrument free of charge.
Extended warranties are also available upon request.
FilterBags
Use ANKOM Technology filter bags (part #’s DF-I, DF-S, DF-FT) in your ANKOMTDF Dietary Fiber Analyzer. Filter bags can
be purchased from ANKOM Technology or from your local authorized ANKOM distributor.
OperatingEnvironment
Your ANKOMTDF Dietary Fiber Analyzer is designed to operate within the following environments:
Ambient Temperature Range: 19°−30°C
Humidity: 20–60% RH
Power: 110V−120V ~ 50/60Hz 15A / 220V−240V ~ 50/60Hz 8A
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If after running this instrument you let it sit unused for 2 weeks or more, you must
clear out the fluid lines to ensure proper operation. See “Appendix C – Storage
Procedure” for more information.
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Operator’sManual
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pg.6 RevA4/30/12
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ContactInformation
Committed to your total satisfaction, we are always available to help you get the most from your ANKOM products and to
receive any comments or suggestions you may have to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
Telephone: [phone]
Fax: [phone]
Email: [email protected]
www.ankom.com
InstrumentDescription
The ANKOMTDF Dietary Fiber Analyzer is designed to efficiently, accurately, and precisely recover IDF, SDF, and TDF
residue from food and/or feed samples in accordance with the AOAC 991.43, AACC 32-07.01, and NMKL 129, 2003 methods.
Enabled by Filter Bag Technology, the instrument can run six digestion and six precipitation processes at the same time.
The filter bags are designed to capture the appropriate fiber particles while allowing all non-fiber components to pass through.
The instrument will use an IDF bag and an SDF bag when configured to recover IDF and SDF residue. The instrument will use
an IDF Flow-Thru bag and an SDF bag when configured to recover TDF residue.
The automated fiber recovery is achieved by first digesting samples within bags using enzyme treatments. Depending on the
filter bag configuration, the instrument then collects the IDF and SDF residue using two separate filters, or collects the TDF
residue using one filter. Once captured in the filters the residue is dried, weighed, and corrected for protein and ash content to
determine the IDF, SDF, and TDF values.
Digestion, precipitation, rinse (H2O and EtOH), and filter operations are performed by the instrument, eliminating manual
transfer and filtration steps. Process temperatures, agitation, and fluid transfer along with digestion, precipitation, and filter
times are precisely controlled by the on-board computer.
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Operator’sManual
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RevA4/30/12   pg.7
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ExternalComponents–FrontView
Touch
Screen
Display
FiltrateCup
(
#1of6
)
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WasteTray
ClampBarD
ClampBarC
HeatingPaddle(#2of6)
MixingPad
(#3of6)
SDFStation(#6of6)
IDFStation(#4of6)
SDFDelivery
Nozzle
(#1of6)
ClampBarA
ClampBarB
Control
Buttons
HookforSDFBag
(#2of6)
IDFDelivery
Nozzle
(#1of6)
ControlPanel
FrontCover
(Clear)

Operator’sManual
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pg.8 RevA4/30/12
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ExternalComponents–LeftSideView
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ExternalComponents–RightSideView
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ControlPanel
95%EtOHContainer
HClContainer
EnzymeContainers
NitrogenSupplyPort
100psi(6.9bar)
FrontCoverArm
PowerSwitch
FrontCover
78%EtOHContainer
BufferContainer
DistilledWaterContainer
FrontCoverArm
ChemicalContainerHolder
HClContainerHolder

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InternalComponents–RearView
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Peristaltic
Pump
Supply
Manifold
Pressure
Sensors
WasteValves
(1–6)
MainElectricalCabinet
ClampBarCylinder
(
1of8
)

In‐LineHeater
“t”Manifold
Water,Buffer,
78%EtOH,
95%EtOH
SupplyValves
IDFInletValve
Stations4,5&6
SDFInletValve
Stations4,5&6
IDFInletValve
Stations1,2&3
SDFInletValve
Stations1,2&3
Enzyme
SupplyValves
HCl
SupplyValve

Operator’sManual
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pg.10 RevA4/30/12
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SafetyPrecautions
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Hot Surfaces – Do NOT touch the Heating paddle surfaces during operation. The
surface can exceed 100°C (212°F). Failure to observe this caution may result in
burns.
Hazardous Voltages – Do NOT operate the instrument with the Main Electrical
Cabinet removed. Hazardous voltages are present during operation. Failure to
observe this caution may result in electrical shock or electrocution.
Hazardous Materials – Ethanol is used within this instrument. Follow both local and
federal regulations for vent hood requirements when operating this instrument. Do
NOT heat seal or place filter bags in an oven until all acetone has evaporated. Handle
Sodium Azide with caution only after reviewing MSDS. Do NOT add Sodium Azide
to a solution until the pH has been adjusted. Failure to observe this caution may be
hazardous to your health.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may result in
serious injury or even death.
This system is designed to meet and/or exceed the applicable standards of CE
and CSA.
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The Power Switch must be in the OFF position before plugging the instrument Power
Cord into the power source.
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Please review the entire manual before you begin operating this instrument.
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