Ankom TDF User manual
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Operator’sManua
l
RevA4/30/12
2052O’NeilRd,MacedonNY14502
Telephone:(315)986‐8090
Fax:(315)986‐8091
www.ankom.com
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TableofContents
Introduction..................................................................................................................................................................5
Warranty.......................................................................................................................................................................5
FilterBags.....................................................................................................................................................................5
OperatingEnvironment................................................................................................................................................5
ContactInformation.....................................................................................................................................................6
InstrumentDescription.................................................................................................................................................6
SafetyPrecautions......................................................................................................................................................10
InstrumentInstallation...............................................................................................................................................11
DietaryFiberAnalysisSupportItems..........................................................................................................................15
AnalysisOptionsusingtheANKOMTDFDietaryFiberAnalyzer...................................................................................15
IDF/SDFAnalysis.........................................................................................................................................................17
TDFAnalysis................................................................................................................................................................30
ProteinDetermination................................................................................................................................................43
AshDetermination......................................................................................................................................................46
Enhanceyourproductivity..........................................................................................................................................49
UnderstandtheStatusScreen....................................................................................................................................53
FaultHandling.............................................................................................................................................................56
DiagnosticsMode.......................................................................................................................................................59
PeriodicMaintenance.................................................................................................................................................71
Troubleshooting..........................................................................................................................................................71
AppendixA–Reagents(AOAC991.43,AACC32‐07.01,NMKL129,2003)................................................................72
AppendixB–StorageProcedure................................................................................................................................74
AppendixC–Parts&Assemblies...............................................................................................................................75
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Operator’sManual
RevA4/30/12 pg.5
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories
around the world in the environmental, agricultural, biomass, and food industries. ANKOM Technology can provide you with
products for determining or monitoring dietary fiber, detergent fiber, fat, digestibility, microbial fermentation (anaerobic or
aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of customer needs.
Congratulations on your purchase of theANKOMTDF Dietary Fiber Analyzer.We are confident that this product will
effectively serve your needs.
The ANKOMTDF Dietary Fiber Analyzer was designed to eliminate most of the manual steps required by the method. This
reduces technician variation, increasing precision. By carefully following the operating instructions in this manual you will
understand the details of sample and filter bag handling as well as the instrument controls, helping you to achieve the best
possible results.
Please review the entire manual before you begin operating this product.
Warranty
ANKOM Technology warrants the ANKOMTDF Dietary Fiber Analyzer against any defects in workmanship or material for one
year after the original date of purchase. This warranty does not include damage to the instrument resulting from neglect or
misuse. During the warranty period, should any failure result from defects in workmanship or materials ANKOM Technology
will, at its discretion, repair or replace the instrument free of charge.
Extended warranties are also available upon request.
FilterBags
Use ANKOM Technology filter bags (part #’s DF-I, DF-S, DF-FT) in your ANKOMTDF Dietary Fiber Analyzer. Filter bags can
be purchased from ANKOM Technology or from your local authorized ANKOM distributor.
OperatingEnvironment
Your ANKOMTDF Dietary Fiber Analyzer is designed to operate within the following environments:
Ambient Temperature Range: 19°−30°C
Humidity: 20–60% RH
Power: 110V−120V ~ 50/60Hz 15A / 220V−240V ~ 50/60Hz 8A
If after running this instrument you let it sit unused for 2 weeks or more, you must
clear out the fluid lines to ensure proper operation. See “Appendix C – Storage
Procedure” for more information.
Operator’sManual
pg.6 RevA4/30/12
ContactInformation
Committed to your total satisfaction, we are always available to help you get the most from your ANKOM products and to
receive any comments or suggestions you may have to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
Telephone: (315) 986-8090
Fax: (315) 986-8091
Email: [email protected]
www.ankom.com
InstrumentDescription
The ANKOMTDF Dietary Fiber Analyzer is designed to efficiently, accurately, and precisely recover IDF, SDF, and TDF
residue from food and/or feed samples in accordance with the AOAC 991.43, AACC 32-07.01, and NMKL 129, 2003 methods.
Enabled by Filter Bag Technology, the instrument can run six digestion and six precipitation processes at the same time.
The filter bags are designed to capture the appropriate fiber particles while allowing all non-fiber components to pass through.
The instrument will use an IDF bag and an SDF bag when configured to recover IDF and SDF residue. The instrument will use
an IDF Flow-Thru bag and an SDF bag when configured to recover TDF residue.
The automated fiber recovery is achieved by first digesting samples within bags using enzyme treatments. Depending on the
filter bag configuration, the instrument then collects the IDF and SDF residue using two separate filters, or collects the TDF
residue using one filter. Once captured in the filters the residue is dried, weighed, and corrected for protein and ash content to
determine the IDF, SDF, and TDF values.
Digestion, precipitation, rinse (H2O and EtOH), and filter operations are performed by the instrument, eliminating manual
transfer and filtration steps. Process temperatures, agitation, and fluid transfer along with digestion, precipitation, and filter
times are precisely controlled by the on-board computer.
Operator’sManual
RevA4/30/12 pg.7
ExternalComponents–FrontView
Touch
Screen
Display
FiltrateCup
(
#1of6
)
WasteTray
ClampBarD
ClampBarC
HeatingPaddle(#2of6)
MixingPad
(#3of6)
SDFStation(#6of6)
IDFStation(#4of6)
SDFDelivery
Nozzle
(#1of6)
ClampBarA
ClampBarB
Control
Buttons
HookforSDFBag
(#2of6)
IDFDelivery
Nozzle
(#1of6)
ControlPanel
FrontCover
(Clear)
Operator’sManual
pg.8 RevA4/30/12
ExternalComponents–LeftSideView
ExternalComponents–RightSideView
ControlPanel
95%EtOHContainer
HClContainer
EnzymeContainers
NitrogenSupplyPort
100psi(6.9bar)
FrontCoverArm
PowerSwitch
FrontCover
78%EtOHContainer
BufferContainer
DistilledWaterContainer
FrontCoverArm
ChemicalContainerHolder
HClContainerHolder
Operator’sManual
RevA4/30/12 pg.9
InternalComponents–RearView
Peristaltic
Pump
Supply
Manifold
Pressure
Sensors
WasteValves
(1–6)
MainElectricalCabinet
ClampBarCylinder
(
1of8
)
In‐LineHeater
“t”Manifold
Water,Buffer,
78%EtOH,
95%EtOH
SupplyValves
IDFInletValve
Stations4,5&6
SDFInletValve
Stations4,5&6
IDFInletValve
Stations1,2&3
SDFInletValve
Stations1,2&3
Enzyme
SupplyValves
HCl
SupplyValve
Operator’sManual
pg.10 RevA4/30/12
SafetyPrecautions
Hot Surfaces – Do NOT touch the Heating paddle surfaces during operation. The
surface can exceed 100°C (212°F). Failure to observe this caution may result in
burns.
Hazardous Voltages – Do NOT operate the instrument with the Main Electrical
Cabinet removed. Hazardous voltages are present during operation. Failure to
observe this caution may result in electrical shock or electrocution.
Hazardous Materials – Ethanol is used within this instrument. Follow both local and
federal regulations for vent hood requirements when operating this instrument. Do
NOT heat seal or place filter bags in an oven until all acetone has evaporated. Handle
Sodium Azide with caution only after reviewing MSDS. Do NOT add Sodium Azide
to a solution until the pH has been adjusted. Failure to observe this caution may be
hazardous to your health.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may result in
serious injury or even death.
This system is designed to meet and/or exceed the applicable standards of CE
and CSA.
The Power Switch must be in the OFF position before plugging the instrument Power
Cord into the power source.
Please review the entire manual before you begin operating this instrument.
Operator’sManual
RevA4/30/12 pg.11
InstrumentInstallation
SiteRequirements
To install and operate the ANKOMTDF Dietary Fiber Analyzer you will need the following:
Adjustable wrench
Adequate power (see “Operating Environment” section of this manual)
Drain or waste container to capture waste fluids
100 psi (6.9 bar) Nitrogen Supply
Because the instrument is powered by pneumatics, do NOT run it without an
adequate Nitrogen Supply. You can run approximately 5 to 10 full assays
(IDF/SDF or TDF) with a 304 cu ft tank of nitrogen.
InstrumentInstallationProcedure
To install the ANKOMTDF Dietary Fiber Analyzer, follow the steps below.
1. Unpackandplacetheinstrumentwhereitwillbeused.
The instrument shipping container consists of three separate cardboard pieces: a top, a bottom, and a sleeve that forms the
body of the container. Remove the top and the sleeve of the instrument shipping container. Lift the instrument off of the
shipping container bottom and place it on a surface that is firm and level in an area near a Nitrogen Supply and a drain o
r
chemical waste container.
Waste fluids are drained from the instrument using gravity. Therefore, position the
instrument so that the drain tube can run down from the Waste Tray.
The instrument must not be subjected to excessive shock, vibration, dirt, moisture, oil, or other fluids.
Ethanol is used within this instrument. Follow both local and federal regulations for
vent hood requirements when installing this instrument.
2. Unpacktheattachmentsandaccessories.
Within the shipping container is a cardboard box containing attachments and accessories. Open the box and verify that all
of the following items are there.
Control Panel
Bag Drying and Weighing: 2 – Desiccant Pouches, 1 – Drying Rack, 1 – Bag Weigh Holder
Operator’sManual
pg.12 RevA4/30/12
Waste Fluid Automatic Elimination: 1 – Waste Tray, 1 – Drain Tube, 1 – Drain Tube elbow connector
Enzyme Containers: 1 – Amylase, 1 – Protease, 1 – AMG
Waste Fluid Collectors: 6 – Filtrate Cups
Chemical Containers with lids: 1 – HCl, 1 – Distilled Water, 1 – Buffer, 1 – 78% EtOH, 1 – 95% EtOH
Chemical Container Holders: 1 – single container holder, 1 – three container holder
3. ConnecttheChemicalContainerHolderstothefeetoftheinstrument.
Remove the nuts from the outside of the left and right feet of the instrument. Slide the small single container holder onto
the bolts on the left foot and slide the large three container holder onto the bolts on the right foot. Secure the holders in
place using the nuts.
Operator’sManual
RevA4/30/12 pg.13
4. ConnecttheControlPaneltotheinstrument.
Remove the nuts from the right side of the instrument. Connect the 25 pin connector from the instrument to the Control
Panel. Slide the Control Panel onto the bolts and secure it in place using the nuts.
5. PlacetheWasteTrayunderthefrontoftheinstrument.
With the Drain Tube connector pointing away from you, slide the tray under the front of the instrument until it can’t go
back any farther. (The side ridges of the tray will contact the front face of the steel frame.)
6. ConnecttheDrainTube.
Connect and secure the Drain Tube to the Waste Tray and run it to either a drain or a waste container, avoiding twists in
the tube. If necessary cut the Drain Tube and use the elbow connector to meet the needs of your location.
ElbowConnector
Boltstoattachthe
threecontainerholder
Boltstoattachthe
singlecontainerholder
Boltsfor
ControlPanel
25pinconnector
Operator’sManual
pg.14 RevA4/30/12
7. PlacetheChemicalContainersintheirholders.
Place the HCl Container in the holder on the left side of the instrument. In the holder on the right side of the instrument,
place the Distilled Water Container in the front section, the Buffer Container in the middle section, and the 78% EtOH
Container in the back section. The 95% EtOH Container sits on the bench behind the container holder.
To ensure proper fluid delivery, you must use the Chemical Containers provided
with the instrument, and place them in their specified locations.
8. ConnecttheChemicalContainerstothecorrespondingtubes.
The Chemical Containers and associated tubes are all labeled and color coded for easy connection using push-on
connectors. Make sure that you hear a click sound when you connect the tubes to the containers.
9. ConnecttheEnzymeContainerstothecorrespondingports.
The Enzyme Containers connect into labeled and color coded ports on the left side of the instrument using push-on
connectors. Make sure that you hear a click sound when you connect the enzyme containers to the ports.
10. ConnecttheNitrogenSupplytotheNitrogenSupplyPort.
11. WiththePowerSwitchintheOFFposition,plugthePowerCordintothepowersource.
Push‐on
Connector
Push‐on
Connector
NitrogenSupplyPort
100psi(6.9bar)
Operator’sManual
RevA4/30/12 pg.15
DietaryFiberAnalysisSupportItems
The following support items needed to perform the fiber analysis may be purchased separately:
ItemANKOMPart#
Electronic Balance with four-place readout TB
Electronic Balance Software TBS
Heat Sealer for sealing the filter bags1915 (120V), 1920 (220V)
IDF Filter Bags DF-I
SDF Filter Bags (used for SDF and TDF procedures) DF-S
IDF Flow-Thru Bags (TDF procedure only) DF-FT
Solvent Resistant Marker F08
Rinse Stand TDF51
Diatomaceous Earth (DE) XTC
AnalysisOptionsusingtheANKOMTDFDietaryFiberAnalyzer
The ANKOMTDF Dietary Fiber Analyzer can be configured to recover IDF and SDF residue, or to recover TDF residue. To
maximize productivity, this instrument also allows a user to start new procedures (IDF/SDF and TDF) before the previous ones
have completed. The following describes these analyses along with their specific filter bag configurations.
Analysis
Name
AnalysisDescriptionUpperBagLowerBag
IDF/SDF This analysis is used to determine IDF and SDF residue separately using
two different filter bags. TDF is calculated by adding the IDF and SDF
residue weights corrected for protein and ash content.
IDF SDF
TDF This analysis is used to determine TDF residue in one filter. In this
analysis, the TDF value consists of the weighed residue corrected for
protein and ash.
IDF Flow-Thru
(no filter)
SDF
A computer controlled Diagnostics mode is provided through the Control Panel for maintenance and troubleshooting purposes.
Operator’sManual
pg.16 RevA4/30/12
Thispageintentionallyleftblank
Operator’sManual
RevA4/30/12 pg.17
IDF/SDFAnalysis
An IDF/SDF analysis measures the amount of IDF and SDF within a given sample. This requires the use of ANKOM DF-I and
DF-S filter bags. When starting a new run, the ANKOM
TDF
Dietary Fiber Analyzer must have bags installed at all stations (a
total of twelve bags).
To enhance the productivity of your instrument, you can begin the IDF process for
a new run while the SDF process of a previous run is finishing. See the “Enhance
your productivity” section of this manual for more details.
To perform an IDF/SDF analysis, follow the steps below.
1. Preparechemicalsandenzymes.
When using the ANKOM
TD
F
Dietary Fiber Analyzer for the AOAC 991.43, AACC 32-07.01, and NMKL 129, 2003
methods use the chemicals and enzymes referred to therein. For your convenience the list of chemicals and enzymes is
located in Appendix A of this manual.
To match the enzyme requirements of the approved method, dilute the standard enzymes referred to in the method with
a
solution of 25% glycerol and 0.02% Sodium Azide in Distilled Water as follows.
EnzymeDilution
Alpha amylase (Megazyme) Dilute 1.25 ml of amylase to 25 ml
Protease (Megazyme) Dilute 2.5 ml of protease to 25 ml
Amyloglucosidase (AMG – Megazyme) Dilute 5.0 ml of AMG to 25 ml
Hazardous Materials – Handle Sodium Azide with caution only after reviewing
MSDS. Do NOT add Sodium Azide to a solution until the pH has been adjusted.
To ensure that you have enough fluids to run a complete IDF/SDF procedure, you must begin with fluid levels above the
Minimum Level lines on the chemical containers and at least 10 ml of each enzyme. Add fluids and enzymes as
necessary.
Do NOT leave the enzyme ports on the instrument open to the air or the enzymes
in the valves may dry up and plug the ports.
2. De‐fatandde‐sugaryoursamplesasneededaccordingtotheAOAC991.43,AACC32‐07.01,or
NMKL129,2003methods.
3. LabelthefilterbagsusingaSolventResistantMarker.
Operator’sManual
pg.18 RevA4/30/12
4. Weighfilterbags.
Using a Bag Weigh Holder is critical to eliminate the effects of static electricity
during the weighing process.
Roll or fold each bag and place it in a tared Bag Weigh Holder. Place the Bag Weigh Holder in the center of a scale and
record the weight.
Because different scales have different sensitivities, the Bag Weigh Holder should
be placed in the center of the scale for best results.
5. WeighDiatomaceousEarth(DE).
DE is used during fiber analysis to enhance the flocculation and filtration of the SDF fraction. Place ca 1 g of DE in each
of six tared tins and record the weights.
6. WeighSamples.
P
lace 0.50.05 g of sample in each of six tared tins and record the weights.
7. InstallSDFfilterbagsontheANKOMTDFDietaryFiberAnalyzer.
7.1 Remove Clamp Bars A, B, C, and D.
7.2 Pull the black SDF Delivery Nozzle out towards you.
ClampBarC
ClampBarD
ClampBarB
ClampBarA
Operator’sManual
RevA4/30/12 pg.19
7.3 Place a labeled and weighed SDF bag up underneath the SDF Delivery Nozzle so that the Delivery Nozzle is
inside the top part of the bag. Pull the bag up so that the top of the bag is about 35 mm above the top of Clamp
Bar C and return the Delivery Nozzle to its original position. This will hold the back of the bag in place.
As part of normal operation, solution from the IDF bag will flow into the SDF bag.
Therefore, when installing the SDF filter bags it is very important to position them
high enough vertically so that at least 20 mm (0.75 inches) of the filter part of the
IDF bag can fit inside the top of the SDF bag.
7.4 With the bag being held by the Delivery Nozzle, center it horizontally between the lines on the back part o
f
Clamp Bar C.
7.5 Repeat steps 7.2 – 7.4 for all six stations.
7.6 Re-install Clamp Bar D.
7.7 Flatten the bags to remove any wrinkles. With fingers away from the clamp bars, press the Clamp Bar D button
on the control panel to pinch the bags just above the filter.
8. AddDEtotheSDFfilterbagsontheANKOMTDFDietaryFiberAnalyzer.
8.1 Open the top of the SDF bag and add a weigh tin of DE to the bag by folding the tin and then dipping it down into
the bag below the tip of the Delivery Nozzle.
When adding DE to the filter bags it is very important to keep it below the tip of
the Delivery Nozzle so that the DE material can be properly rinsed.
CenteringLines
Operator’sManual
pg.20 RevA4/30/12
8.2 Rinse the tin with no more than 3 ml of Distilled Water to ensure complete transfer.
8.3 Repeat steps 8.1 – 8.2 for all six stations.
9. InstallIDFfilterbagsontheANKOMTDFDietaryFiberAnalyzer.
9.1 Pull the black IDF Delivery Nozzle out towards you.
9.2 Place a labeled and weighed IDF bag up underneath the Delivery Nozzle so that the Delivery Nozzle is inside the
top part of the bag. Pull the bag up so that the top of the filter part of the IDF bag is just below the bottom o
f
Clamp Bar B and return the Delivery Nozzle to its original position. This will hold the back of the bag in place.
9.3 With the bag held by the Delivery Nozzle, center it horizontally between the Centering Lines on the back part o
f
Clamp Bar A.
For proper mixing during the IDF process the IDF bags must be horizontally
centered over the Heating Paddles and between the Centering Lines on the back
part of Clamp Bar A.
9.4 Place at least 20 mm (0.75 inches) of the filter section of the IDF bag inside the top of the SDF bag to allow fo
r
the flow of liquid into the SDF bag after the IDF process is complete.
9.5 Repeat steps 9.1 – 9.4 for all six stations.
9.6 Re-install Clamp Bar B.
9.7 Flatten the IDF bags to remove any wrinkles. With fingers away from the clamp bars, press the Clamp Bar B
button on the control panel to pinch the IDF bags just above the filter. The mixing pads will make contact with the
bags when Clamp Bar B pinches the IDF bags.
9.8 Re-install Clamp Bar C.
CenteringLines
HeatingPaddle
ClampBarB
Toppartofthefilteris
positionedjustbelowthe
bottomofClampBarB20mm(0.75inches)of
theIDFfilterinsidethe
topoftheSDFbag
HeatingPaddle
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