ATAGO Po-1 User manual

5050-E01
Portable Polarimeter
Instruction Manual
Cat. No. 5050

クイックマニュアル
3 Measurement and Evaluation
② (rpage 6・page 7)
①Wipe the sample stage clean.
②Fill the sample stage ith the sample
②solution up to the fill line.
③Press F.
The measurement value is displayed. The green
/red
indicators notify whether the measurement falls
within or outside the user-programmed tolerance
setting. (rpage 7)
N
See page 8 for cleaning tips.
N See page 9 for how to change the settings.
Quick Manual
1
Powering on
(rpage 4)
①Press I.
2 Zero setting (rpage 5)
①Wipe the sample stage clean.
②Fill the sample stage ith ater up to the fill
②line.
③Press G.
③000 is displayed.
The cursor indicates the last used
scale.
Sample volume
Fill line
( pprox. 3mL)
Measurement
evaluation indicator

Multi-scale: Angle of Rotation; Specific Rotation; Concentration
Main Features
Press H to select the des
ired scale
–
Specific Rotation (factory default)
; or
Concentration
The One and Only Polarimeter That oesn’t Use Observation Tubes
The instrument measures angle of rotation equivalent to a 20mm observation tube and
displays angle of rotation converted to 100mm observation tube and 200mm observatio
n
tube.
Measurement Evaluation Indicator
The measurement values are evaluated against the pre-set tolerance, and the green/red
light will indicate the results.
See page 10 for how to configure upper and lower limits.
Red light
= Outside tolerance
Green light
= Within tolerance
O
utside
tolerance
Red light Green light Red light
Within
tolerance
Outside
tolerance
Upper limit Lo er limit
Angle of Rotation
（Conv
ersion to 100mm
observation tube ）
Angle of Rotation
（
Conversion to 200mm
observation tube ）
Angle of Rotation
（
Conversion to 100mm
optical path length）
Angle of Rotation
（
Conversion to 200mm
optical path length）

Useful Features
Turn on Backlight
Backlight stays on for 1 minute after any button is pressed.
View Scale Information
Change Scale
During measurement: Press START to display the scale name abbreviation next to the
battery indicator.
After measurement: The cursor indicates the last used scale.
Concentration
Press the desired scale button.
: ngle of Rotation (Conversion to 100mm optical path length)
: ngle of Rotation (Conversion to 200mm optical path length)
H: Every time H is pressed, the other scales cycle through in the following order:
Specific Rotation→Concentration
Then, the measurement in the selected scale is displayed.
fter measurement: Press one of the scale buttons to display the following scale name abbreviation next to the
battery indicator.
Specific
Rotation
ngle of
Rotation
（Conversion to 100mm
optical path length）
Adjust Auto Shut-off Timer
(rpage 13)
Is the unit turning itself off too soon?
g
Choose from timer options of 4, 10, or 30
minutes.
Sr
℃
con
℃
100
℃
200
℃
ngle of Rotation
（
Conversion to 100mm
optical path length）
ngle of Rotation
（
Conversion to 200mm
optical path length）
User
(Specific Rotation. Concentration)
ngle of
Rotation
（Conversion to 200mm
optical path length）

Contents
Precautions for use················································································································· 1
1. Overview·································································································································· 2
1. Contents·········································································································································································2
2. Components and Functions ·································································································································2
3. Battery Replacement···············································································································································4
4. Po er ON/OFF··························································································································································4
2. Basic Operations················································································································· 5
1. Zero-setting ·································································································································································5
2. Measurements·····························································································································································6
3. Cleaning ··································································································································· 8
4. Settings···································································································································· 9
Ho to Access Settings··············································································································································9
1. Tolerance Setting····················································································································································10
2. Setting the Optical Path Length······················································································································11
3. Concentration/Specific Rotation Setting···································································································12
4. Auto Shutdo n Timer Setting ··························································································································13
5. Scales ·····································································································································14
. Error Messages ··················································································································15
7. Inspection······························································································································1
8. Storage and Maintenance ·····························································································1
9. Optional Accessories and Consumable Items·····················································17
10. Warranty and Repair······································································································18
1. Warranty·······································································································································································18
2. Replacement Parts·················································································································································18
11. Specifications···················································································································19

- 1 -
Precautions for use
ntroduction
Thank you for purchasing the instrument. Carefully read and follo all instructions. Keep this manual for
future reference.
Safety nstructions
Read and follo all safety instructions before operating the instrument.
Failure to comply ith the follo ing instructions may result in personal injury or property damage.
 WARN NG
Ensure safety when handling hazardous materials.
Observe precautionary measures and use protective
equipment, Be aware of the hazards of such chemicals
and emergency response guidelines.
Do not drop the instrument or subject it to strong
physical shock.
T GO may not be held liable for any injury or damage
arising in connection with handling of hazardous
materials during the use of the instrument.
Do not attempt to repair, modify, or disassemble the
instrument.
 CAUT ON
Carefully read this manual to have basic knowledge of
the function of each component.
Some acids may corrode the
optical path window,
and/or (metal) sample stage, which may cause
erroneous measurements.
Do not use metal tools, such as a spoon, as they may
scratch the optical path window, resulting in erroneous
measurements.
Only use the specified battery type. Observe proper
polarities, properly aligning the anodes and cathodes.
Store the instrument away from direct sunlight/heat
sources and excessive amounts of dust/debris.
Store the instrument in a dry environment.
Do not expose the instrument to a rapid change in
ambient temperature.
Do not subject the instrument to strong vibration.
Do not subject the instrument to extreme cold
temperature.
Do not place the instrument under anything heavy.
Have the batteries and battery compartment cover
removed during air transport.
〈Chemical Resistance of Body Case〉
The body case is made of BS resin. Do not expose it to water vapor or solvents.
Aqua regia chromic acid chlorosulfuric acid hydrobromic acid nitric acid hydrofluoric acid sulfuric acid phosphoric acid ethyl
acrylate butyl acrylate ethyl acetoacetate acetophenone benzyl benzoate ethylbenzene ethylene oxide ethylenediamine
ethylene chlorohydrin epichlorohydrin ethyl chloride benzyl chloride methyl chloride chlorinated solvents xylene (xylol) cresol
chloroacetone chlorotoluene chloroform acetic acid amyl acetate isopropyl acetate ethyl acetate butyl acetate propyl acetate
methyl acetate diphenyl oxide diisopropyl ketone carbon tetrachloride dioxane cyclohexanone (anone) dichlorobenzene dibutyl
phthalate (DBP) dimethylformamide (DMF) dimethylaniline phenol thioalcohol (mercaptan) tetrahydrofuran (THF) trichloroethylene
toluene (toluol) ethylene dichloride dichloromethane nitroethane nitropropane nitrobenzene nitromethane perchlorethylene
fluorobenzene methyl methacrylate methyl isobutyl ketone methyl ethyl ketone monochloroacetic acid monochlorobenzene liquid
chlorine thionyl chloride sodium peroxide bromine benzene lacquer
m Solvents that are harmful to the plastic body case include but are not limited to the above substances.

- 2 -
1. Overview
1. Contents
・ Main unit···························································1 ・ AAA batteries·················································4
・ Instruction Manual (this book)·················1 ・Disposable plastic pipettes························3
2. Components and Functions
ST RT button (F)
Take a measurement.
Select a setting item. POWER button (I)
Turn on in the measurement mode.
LCD
Displays the measurement values,
temperature/scale, and battery indicator.
Sample stage
pply the sample to be measured.
Fill line
Indicates a sufficient
amount of liquid.
Optical path window
Polarized light is
emitted and received.
Measurement Evaluation Indicator
Either the green or red light comes on when the
measurement value is within or outside the
tolerance range respectively.
Scale button
ZERO button (
G
)
ZERO set.
Confirm the selection.
100mm button ( )
Select the angle of rotation scale
(Conversion to 100mm optical path length).
Move to the next place value.
200mm button ( )
Select the angle of rotation scale
(Conversion to 200mm optical path length).
Increase the value.
User button (H)
Select from : Specific Rotation; or Concentration
Decrease the value.
Measurement modes =
Settings =

- 3 -
LCD
Bottom vie
Battery compartment
Place and remove batteries
from here.(rpage 4)
Temperature/Scale
The scale, and then,
the temperature is displayed for
each measurement.
Cursor
The cursor indicates the last used scale.
Scales
100mm : Angle of Rotation
（Conversion to 100mm
optical path length）
200mm : Angle of Rotation
（Conversion to 200mm
optical path length)
User : User
(Specific Rotation,
or Concentration)
Measurement value
Battery indicator

- 4 -
3. Battery Replacement
 CAUTION
●
When the battery icon indicates the lo po er level ( ), replace all four batteries
ith a brand ne set of AAA alkaline batteries (1.5V).
●
Check the expiration dates on batteries before purchase.
●
Remove the battery from the unit hen the unit is not to be used for a month or so.
①Turn the screw counterclockwise
with a flat-head screwdriver or a
coin to open the battery
compartment.
②Insert the batteries, observing the
correct polarity.
③Close the battery compartment and
turn the screw clockwise.
4. Power ON/OFF
Powering on
①Press I.
Turn the instrument on in the measurement mode.
The backlight stays lit for 1 minute.
The cursor indicates the last used scale.
N The backlight stays on for 1 minute after any button
is pressed.
N To access the settings, while the power is turned on,
press and hold H, then 1 second later, press
I (
r
page 9).
Powering off
①Hold do n I.
The screen ill turn off.
N The instrument will turn itself off after 4 minutes of
inactivity by default.
The auto shutdown timer can be extended to 10
minutes or 30 minutes (
r
page 13).
g g
g
Open Close

- 5 -
2. Basic Operations
 CAUTION
●
Operate a ay from direct sunlight at a stable temperature ith as little fluctuation
as possible.
●
Acclimate both the instrument and sample solution to room temperature to ensure
accurate results. Measurements may fluctuate ith hot or cold samples. Wait to
press
F
after placing the sample on the sample stage. Alternatively, press
F
multiple times.
●
Operate on a flat ork surface.
●
The displayed temperature is that of the sample stage and may not necessarily
match the temperature of the sample.
1. Zero setting
This is the operation to set the calibration point (zero).
m Zero-set the instrument at the beginning of each day before use.
m Regular zero-setting throughout the day is recommended to ensure accurate results.
①Press I to turn it on.
m Zero setting can be done in any
scales.
②Wipe the sample stage clean.
③Fill the sample stage ith tap ater up to
the fill line.
N Use room temperature tap water. The
instrument should also be acclimated to
room temperature prior to operations.
④Press G.
000 flashes and stops.
Fill line
( pprox. 3mL)

- 6 -
2. Measurements
①Wipe the sample stage clean.
②Fill the sample stage ith the sample
solution to the fill line.
③Press F.
---flashes, and then the scale name is
displayed next to the battery indicator.
Then, the measurement value and
temperature ill be displayed.
N
Measure room temperature (distilled)
samples. The instrument should also be
acclimated to room temperature prior to
operations.
N Try stirring the sample on the sensor
during measurement to improve the
repeatability of oily/fatty samples.
Fill line
(Approx. 3mL)
m
The measurement evaluation indicator will
light up if a tolerance is set for the scale
used (
r
page 10).
m The indicator will not activate for
erroneous results (including temperature
errors).
m Set the upper and lower limits to 0.00 to
turn off the measurement evaluation
indicator.

- 7 -
〈Measurement Evaluation ndicator〉
Example: When the Angle of Rotation (conversion to 200mm optical path length) is Configured ith a
lo er limit of 33.0% and an upper limit of 36.0%.
①Select the Angle of Rotation (conversion to
200mm optical path length) scale.
②Press F to take a measurement.
--- ill appear.
Sample A Sample B Sample C
32.0° 34.6° 37.5°
g
ggg
Red light Green light Red light
Sample: Sucrose solution (26.0g/100mL)
+33.0 to +36.0°
Outside tolerance Within tolerance Outside tolerance
Red light Green light Red light
Outside tolerance
32.0°
Sample A
Within tolerance
34.6°
Sample B
Outside the tolerance
37.5°
Sample C
Red/Green Light Indication

- 8 -
3. Cleaning
 CAUTION
●
Ensure that the sample stage is clean before measurement.
●
Clean the sample stage immediately after each measurement.
●
Thoroughly dry the instrument before storing.
●
Do not use benzene (paint thinner) on the instrument.
●
Store a ay from direct sunlight at a stable temperature ith as little fluctuation as
possible.
①Collect the sample
solution ith a pipette
and ipe it off.
③Dry the sample stage
completely ith dry
tissues.
Ethyl
alcohol
Mild soap
For oily samples
Clea
n oily residues ith ethyl
alcohol or mild soap, and then,
rinse ith ater.
②Apply some ater and
ipe it off.
a
a
N
Clean and the
optical path window
particularly well.
Optical path indo
Can be ashed under running ater.
Thoroughly dry the instrument before
storing.
N
Moisture may accumulate in the
grooves of the buttons, but this
will not affect the instrument.

- 9 -
4. ettings
How to Access Settings
This section ill explain ho to change settings after accessing the menu.
G
F
G
F
G
F
G
F
-
E
.
n
.
d
-
.
in
.
P
[2]
-
8.
J
.
U
[1]
-
8.
A
.
P
[3]
Exit the settings. Return to the measurement mode.
②Press F to
move to the next
setting item.
③Press G to select the setting.
1. Tolerance Setting (
r
page 10)
2. Setting the Optical Path Length
（rpage 11）
3. Concentration/Specific Rotation
Setting (rpage 12)
4. Auto Shutdo n Timer Setting
(rpage 13)
Return to [ 1] JU.
①While the po er is
turned on, press and
hold “user”, then 1
second later, press
“Po er”.
H
↓
after 1 second
H＋I

- 10 -
1. Tolerance Setting
Set upper and lower limits for a scale, and the indicator will light up to notify if a measurement falls within or outside the tolerance.
N For angle of rotation, switch scale in measurement mode.
Range: -999 to +999
Resolution
(regardless of positive or negative value) :
> 100 : 1
10.0 to 99.9 : 0.1
< 9.99 : 0.01
[Factory default: lower limit = -25.0，
upper limit = +25.0 in ngle of Rotation
(Conversion to 100mm optical path length)]
G
Confirm the
scale
.
"
Ｏ
ptical path length
" will flash. (For
angle of rotation.)
G
Confirm the upper limit.
How to access the setting (rpage 9)
-
c
J
.
U
[1]
G
① →While ⑴ is flashing a Select the sign by or
H .
② →While ⑵ is flashing a Increase the value by .
a Decrease the value by
H .
③ →While ⑶ is flashing a Choose to insert a decimal point by
or
H .
④ →While ⑷ is flashing a Same as ② above.
⑤ →While ⑸ is flashing a Same as ③ above.
⑥ →While ⑹ is flashing a Same as ② above.
⑵ Value
-
0
.
0
.
0
S
H
..
i
Enter the upper limit.
⑹ Value
⑸
Decimal
⑸ point
⑷ Value
⑶
Decimal
⑸ point
-
4
.
0
.
0
S
L
..
o
Enter the lower limit.
-
E
.
n
.
d
G
Return to the measurement mode.
G
Confirm the lower limit. “SEt” will flash on the display.
F
-
c
J
.
U
[1]
Exit the setting.
N Press G to continue to edlit
the setting.
N The tolerance range is different
from the display range.
m Set the upper and lower limits to
0.00 to turn off the measurement
evaluation indicator.
N
The last used scale will be selected
upon returning to the measurement
mode.
⑴
Sign
N
When cancelling settings, press
F
. Proceed
to lower limit value input.
N
When cancelling settings,
press F .
F
F
-
S
.
r
S
J
.
U
Specific Rotation
-
r
.
o
.
t
S
J
.
U
ngle of Rotation
F
-
c
.
o
.
n
S
J
.
U
Concentration

- 11 -
2. Setting the Optical Path Length
The instrument measures angle of rotation equivalent to a 20mm observation tube.
When using the Reduced Optional Path Adapter (optional accessory), set the Reduced Optical Path
Adapter's optical path length (as 5mm or 10mm).
-
CEL
S
r
nP
G
How to access the setting (rpage 9)
-
i
.
n
.
P
[2]
G
-
E
.
n
.
d
G
Return to the measurement mode.
G
Confirm the value. “SEt” will flash on the display.
F
-
i
.
n
.
P
[2]
Exit the setting.
N Press G
to continue to edit the
setting.
N
The last used scale will be selected
upon returning to the measurement
mode.
[Factory default: 20mm]
F
Do not select here. Do not select here.
F
F
S
r
nP
-
c
.
o
.
n
S
r
nP
-
S
.
r
.
F
20mm
F
-
b
.
2
..
0
CEL
-
b
.
3
..
5
CEL
-
b
.
1
..
0
CEL
F
10mm 5mm

- 12 -
3. Concentration/Specific Rotation Setting
Range: Concentration 0.00 to 999
Range: Specific Rotation -999 to +999
Resolution
(regardless of positive or negative value) :
> 100 : 1
10.0 to 99.9 : 0.1
< 9.99 : 0.01
-
0
.
0
.
0
S
con
G
Confirm the scale.
How to access the setting (rpage 9)
-
i
.
n
.
P
[2]
G
① →While ⑴ is flashing a Select the sign by or
H .
② →While ⑵ is flashing a Increase the value by .
a Decrease the value by
H .
③ →While ⑶ is flashing a Choose to insert a decimal point by
or H .
④ →While ⑷ is flashing a Same as ② above.
⑤ →While ⑸ is flashing a Same as ③ above.
⑥ →While ⑹ is flashing a Same as ② above.
⑵ Value
Enter the value.
⑹ Value
⑸
Decimal
⑸ point
⑷ Value
⑶
Decimal
⑸ point
-
E
.
n
.
d
G
Return to the measurement mode.
G
Confirm the value. “SEt” will flash on the display.
F
-
i
.
n
.
P
[2]
Exit the setting.
N Press G
to continue to edit the
setting.
To measure Concentration a
enter Specific Rotation
To measure Specific Rotation a
enter Concentration
N Both variables can be configured.
N
The last used scale will be selected
upon returning to the measurement
mode.
⑴
Sign
[Factory default: Concentration of 26.0g/
100mL，Specific Rotation of 66.6°]
N
When cancelling settings,
press F .
Concentration Specific Rotation
-
c
.
o
.
n
S
r
nP
-
S
.
r
.
S
r
nP
-
CEL
SrnP
F
F
F
Do not select here.

- 13 -
4. Auto Shutdown Timer Setting
Choose from the follo ing 3 timer options.
F
G
How to access the setting (rpage 9)
-
8.
A
.
P
[3]
[Factory default: 4 minutes]
G
Confirm the selection. “SEt” will flash on the display.
-
8.
A
.
P
[3]
-
E
.
n
.
d
Turn off after 4 minutes of inactivity
G
F
-
r.
o
4
.
AP
-
b
.
3
..
0
AP
-
b
.
1
..
0
AP
F
Turn off after 10
minutes of inactivity
F
Exit the setting.
N
The last used scale will be selected
upon returning to the measurement
mode.
Turn off after 30
minutes of inactivity
N Press G
to continue to edit the
setting.
Return to the measurement mode.

- 14 -
5. cales
ANGLE OF ROTATION - denoted by "α" and expressed in degrees ("° ")
The rotation of polarized light is observed when polarized light that is oscillating unidirectionally rotates as it passes
through a liquid. The measurement of the angle of such rotation is called the " ngle of Rotation."
The angle of rotation is the basic numeric value measured by polarimeters and are dependent on the concentration
of the liquid, the length of the observation tube, temperature, and the measurement wavelength.
The instrument measures angle of rotation equivalent to a 20mm observation tube and displays angle of rotation
converted to 100mm observation tube and 200mm observation tube.
SPECIFIC ROTATION - Denoted by "[α]" and expressed in degrees ("° ")
The Specific Rotation is a numeric value unique to each substance. By definition, the specific rotation value is equivalent
to the angle of rotation obtained by a measurement using a solution of 100% concentration in a 100mm observation tube
(although such a measurement is actually not practicable). The specific rotation is determined by the following formula:
Specific Rotation = (10,000 × ngle of Rotation)/(length of observation tube [mm] × Concentration [g/100mL]
*1
)
*1
m To measure the specific rotation, input the concentration value of the
※1
m sample to be measured into the instrument before taking a measurement. (
r
page 12)
CONCENTRATION - Expressed in g/100mL
The Concentration is the density of an optically active substance dissolved in water or alcohol. The concentration is
determined by the following formula:
Concentration = (10,000 × ngle of Rotation)/(length of observation tube [mm] × Specific Rotation
*2
)
*2
m To measure the concentration, input the specific rotation value of the
※2
m sample to be measured into the instrument before taking a measurement. (
r
page 12)