ATILA Powergene Power96-1 User manual

Atila Powergene Real-Time PCR Systems User Manual Page 2
Safety and Regulatory Compliance
Important Safety Information
Please read these instructions before operating the Atila Powergene system.
Electrical Safety Precautions
Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live
circuit, xture, receptacle, or switch. Safety rules you should follow whenever working with any electrical
appliance include:
• Always shut off power at the main disconnect before changing a fuse.
• Always shut off power to the circuit before repairing or replacing a switch, receptacle, or xture.
• Always tape over the main switch, empty fuse socket, or circuit breaker you are working on.
• Always check that the circuit is dead before beginning work on it. Using a circuit tester or voltmeter
can help you determine this.
• Always unplug any appliance before repairing it.
Protective ground terminal
The ground terminal, intended for connection to an external protective conductor for protection
against electric shock in case of a fault, is located on the inside of the back panel.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case the user
will be required to correct the interference at his own expense.
Changes or modications not expressly approved by the party responsible for compliance could void the
user’s authority to operate the equipment.
Hot surface warning
Under normal conditions, the temperature of plate is below 50°C and safe to touch. However,
if the system malfunctions, it is possible that the glass surface temperature exceeds 80°C.
Please exercise caution when touch the plate surface with hand when this occurs.
CAUTION: No user serviceable parts inside!
For Research Use Only
This instrument is suitable for research use only. It must be used, therefore, only by personnel who know the
health risks associated with the reagents that are normally used with this instrument.

Atila Powergene Real-Time PCR Systems User Manual Page 3
Warranty
The Atila Powergene products are warranted against defects in materials and workmanship for one year
unless otherwise outlined on your sales order . If any defect occurs in the instrument during this warranty
period, Atila Biosystems, Inc. will repair or replace the defective parts at its discretion without charge. The
following defects, however, are specically excluded:
•Defects caused by improper operation.
•
Repair or modication done by anyone other than Atila Biosystems or the company’s
authorized agent.
•Use of spare parts supplied by anyone other than Atila Biosystems.
•Damage caused by accident or misuse.
•Damage caused by disaster.
•Corrosion caused by improper solvents or samples.
Voltage Setting Information
The Atila Powergene system has apower supply that automatically chooses the correct voltage for your
country or region.
CE Conformity
The following Powergene Model Systems ,
models : Power 96-1
and Power96-2 are in conformity with the
provisions of the following EC Directives , including all amendments , and national legislation implementing
these directives:
• EN61010-1: 2010
• EN61326-1: 2013
• EN61326-1:2013
• EN301489-1 v2.2.1:2019
• EN301489-17 v3.2.0:2017
• EN300238 v2.1.1:2016
Protection category: IP20 according IEC 60529

Atila Powergene Real-Time PCR Systems User Manual Page 4
ENG
This symbol indicates that the waste of electrical and electronic equipment must not
be disposed as unsorted municipal waste and must be collected separately. Please
contact an authorized representative of the manufacturer for information concerning
the decommissioning of your equipment.
FRA
Ce symbole indiquequelesdéchetsrelatifsàl’équipementélectriqueetélectronique
ne doivent pas être jetés commeles orduresménagèresnon-triées et doiventêtre
collectés séparément. Contact ezunreprésentant agréédu fabricant pour obtenir
desinformations sur lamise au rebut de votre équipement.
GER
Dieses Symbol kennzeichnet elektrische und elektronische Geräte,dienichtmit dem
gewöhnlichen, unsortierten Hausmüll entsorgtwerdendürfen,sondernseparatbehan
deltwerdenmüssen.BittenehmenSieKontakt miteinemautorisierten Beauftragtendes
Herstellers auf, umInformationen hinsichtlichder EntsorgungIhresGerätes zu erhalten.
ITA
Questosimboloindicacheiriutiderivantidaapparecchiatureelettricheed elettroniche
nondevonoessere smaltiti comeriuti municipaliindifferenziati e devono invece esser-
eraccoltiseparatamente.Perinformazionirelativeallemodalità di smantellamentodell-
eapparecchiaturefuoriuso,contattareunrappresentanteautorizzatodelfabbricante.
SPA
Estesímbolo indica que elequipoeléctricoy electrónicono debe tirarseconlosdesechos-
domésticosydebetratarseporseparado.Contacteconelrepresentantelocaldelfabricante-
paraobtenermásinformaciónsobrela forma de desecharel equipo.
SWE
Dennasymbolangerattelektriskaochelektroniskautrustningarintefåravyttrassomosort-
erathushållsavfallochmåstesamlasinseparat.Vargodkontakta en auktoriserad till-
verkarrepresentant för informationangåendeavyttringavutrustningen.
Waste Electrical and Electronic Equipment (WEEE)
Contact
Atila Biosystems, Inc.
740 Sierra Vista Ave, Suite E • Mountain View, CA 94043 • USA
[e-mail] • [phone]

Atila Powergene Real-Time PCR Systems User Manual Page 1
Table of Contents
1. Introduction 2
1.1 Atila Powergene Specications 2
1.2 Contacting Technical Support 2
2. Installation and Setup 3
2.1 System Placement 3
2.2 Connecting to Power 3
2.2 Turning the System On/Off 3
2.2 Software Installation 3
3. Device Software Overview 4
3.1 The Home Screen 4
3.2 Experiment Setup 4
3.2.1 Change Protocol Properties 5
3.2.2 Temperature Settings 6
3.2.3 Time Settings 7
3.2.4 Cycle Settings 7
3.2.5 Advanced Settings 8
3.2.6 Saving Run Setup Settings 9
3.2.7 Run Experiment: Real Time 9
3.3 Data Manager 9
3.4 Device Settings 10

Atila Powergene Real-Time PCR Systems User Manual Page 2
1. Introduction
The Atila Powergene Real-Time PCR instrument is a high performance tool compatible with a wide
variety of reagents for qPCR . The family of instruments includes the Atila Power 96-1 and the Atila
Power96-2.
1.1 Atila Powergene Specications
The Atila Powergene instruments have the following specications.
Specications Atila Power96-1
Real Time PCR System
Atila Power96-2
Real Time PCR System
Part number Power96-1 Power96-2
Product description
96-well Real-Time PCR instrument
with 5 dye channel lters
96-well Real-Time PCR instrument
with 10.2” touchscreen interface,
6 dye channel lters
Sample capacity (wells) 96 96
Reaction volume 10–150µL 10–150µL
Excitation source PMT LED
Detection channels 56
Multiplexing Up to 5targets Up to 6 targets
Thermal element Peltier Peltier
Max. block ramp rate 6°C/sec 6°C/sec
Avg. sample ramp rate 4°C/sec 4°C/sec
Temperature uniformity ±0.2°C ±0.2°C
Temperature accuracy ±0.1°C ±0.1°C
Dye compatibility
FAM, HEX, Rox,Cy5, Cy5.5
SYBR Green, EvaGreen, FAM,
VIC, JOE, HEX, CAL Fluor 540,
CAL Fluor Orange 560, ROX,
TAMRA, TEX615, Quasar 670,
CAL Fluor Red 610, Cy5, LIZ, Mustang
Purple, Cy5.5, Quasar 705
Custom dye/chemistry üüüü
Chemistry capability Fast/Standard Fast/Standard
Detection sensitivity 1 copy 1 copy
Sensitivity Detect differences as small as 2-fold in
target quantities in singleplex reactions
Detect differences as small as 2-fold in
target quantities in singleplex reactions
Connectivity USB, Wi-Fi, Ethernet USB, Wi-Fi, Ethernet
1.2 Contacting Technical Support
For questions about installation, setup or general use of the system, please contact
suppor[email protected] or call [phone], 9am–6pm Pacic time.

Atila Powergene Real-Time PCR Systems User Manual Page 3
2.1 System Placement
WARNING: Excessive Weight Hazard – Please use two or more people to lift the system. Failure to do so
can result in system damage and personal injury.
As with all electrical instruments, the Atila Powergene should be located away from water, solvents, or
corrosive materials, on a at and stable surface with adequate clearance on all sides. The system must
remain stationary during operation.
The system should be placed away from interfering electrical signals and magnetic elds. If possible, a
dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in
your laboratory.
The Atila Powergeneshould be installed at no more than 3000 meters above sea level.
2.2 Connecting to Power
The power entry module is located in the lower right on the back panel of the system.
Connect the power cord to a secure power outlet.
It is important to connect the system to a well-grounded power source. Atila
Biosystems recommends that you employ a surge protecting power strip to
protect against unexpected power surges that may cause damage to the
electrical components.
2.3 Turning the System On/Off
The main power switch is located at the back panel, next to the power cord.
1. First, ip the power switch to On (l).
2. To wake the integrated computer, press the power button on the front of the system
and hold for 3 seconds. The machine will signal it is powering on with a loud beep
noise. The power button on the front of the system will light up green when the
system is on.
3. To turn off the system, hold the power button on the front for 5 seconds until the
device powers off, then ip the power switch on the back to off.
Atila Biosystems recommends leaving the system on during working hours.
2.4 Software Installation
The Atila Powergene Device software is pre-installed. Once the system is done initializing, the software
displays and is ready to use.
Please contact Atila Technical support at suppor[email protected] for questions about updating
the software.
2. Installation and Setup

Atila Powergene Real-Time PCR Systems User Manual Page 4
The Atila Powergene Icon based software allows you program
and run protocols with a simple user interface.
3.1 The Home Screen
• Experiment Setup—for setting up a new run, or running an
existing protocol, see section 3.1.1.
• Data Manager—for accessing run data, see section 3.1.2
• Device Settings—access device settings, see section 3.1.3.
3.2 Experiment Setup
1. Tap this symbol next to Users or Protocol and Date
created to sort users and/or experiments in ascending
or descending order.
2. Add new user:
• Tap symbol to add a new user.
• Tap on ‘Add User’ to add a new user. Enter the new
user name using the system keyboard.
3. Add a new protocol:
• Tap symbol to add a new protocol.
• Tap on ‘Add Protocol’ to add a new user.
Enter the name for the protocol using the system
keyboard.
• You can see the new user or new protocol added to
the PROTOCOL MANAGER screen.
• The Preview window displays the default
experiment settings.
3. Device Software Overview

Atila Powergene Real-Time PCR Systems User Manual Page 5
4. Delete existing user or protocol:
• Tap on the user or protocol to be deleted.
• Tap this symbol to delete the selected user or
protocol.
Note: Deleting user will delete all existing protocol and
data stored under that user.
• The system will prompt you to conrm the deletion.
Tap ‘OK’ to delete.
3.2.1 Change Protocol Properties
1. Tap to select the protocol to edit properties.
2. To edit the selected protocol tap .
3. Tap on the rectangular text box with the protocol
to assign a name (or rename) the protocol.
• For Example, ‘cell line b’ was added to the text
box eld using system keyboard.
4. There are various buttons for Volume, Temperature
and Light Source.
• Tap on the button to select particular volume,
temperature and light source.
• The selected button will turn green from this
default color blue.
• To switch off heat, tap on the dark red ‘OFF’
button. The button is bright red when heat
is off .
5. Tap on to go back to PROTOCOL MANAGER
Screen.
6. Use the ‘EMAIL’ button to send the Properties data
to an email address. Enter the email address and
tap on ‘OK’ to send. Press cancel to exit the overlay
and return to the Properties screen.
7. Tap on ‘WELL SELECTION’ to select wells from a
96 well PCR plate.

Atila Powergene Real-Time PCR Systems User Manual Page 6
8. A screen with a diagram of 96 well PCR plate
appear on the screen. By default, all wells are
selected (in blue).
• To deselect empty well, tap on the well to clear
selection. Press ‘OK’ to save changes on the
well plate and return to Properties screen.
• You may also use ‘CLEAR ALL’ and tap to
select the wells. Press ‘OK’ to save changes on
the well plate and return to Properties screen.
9. Press ‘OK’ to save changes on the well plate and
return to Properties screen.
10. Tap to proceed to RUN SETUP.
• Tap on the switch button to turn the Melt
Curve OFF. [When blue, the Melt curve is ON;
When gray the Melt curve is OFF].
3.2.2 Temperature Settings
1. Tap on the temperature display button for each
cycle to change the temperature(°C). Enter the
numerical digits [Example: 60] using the key pad
on the screen. Press ‘DONE’ to save.
2. Tap to clear the entered numerical digits.
Example: Deselected H7 and
H8 wells.
Example: Select only well H7 and H8.

Atila Powergene Real-Time PCR Systems User Manual Page 7
3.2.3 Time Settings
1. Tap on the time display button for each cycle to change the time. Enter the temperature using
the key pad on the screen.
• Example: to set the time to 2 minutes, Tap ‘Min’ on the screen and enter the number 2
using the key pad on the screen. Press ‘DONE’ to save.
• Example 2: to set time to 1 minutes 30 seconds, tap on ‘Min’ on the screen and enter the
number 1. Tap on ‘Sec’ and enter the number 30 using the key pad on the screen. Press
‘Done’ to save.
2. Tap to clear the entered numerical digits.
3.2.4 Cycle Settings
1. Tap on the purple display button under the CYCLING stage. The number on the button
displays the number of cycles.
2. Tap on ‘CYCLES’ to enter the number of cycles you want to run.
3. Tap on ‘GO TO’ to enter the step number you want the cycle to repeat every time, after the
last step of the cycle.
• Example: To set 40 cycles consisting of step 2 and 3, tap on the purple button that
displays number of cycles. Tap on CYCLES and enter 39. Tap on ‘GO TO’, and enter 2
(Step number).
• Note: The cycle count in the system starts at 0. Thus, 0 to 1 is considered as 1 cycles.
Thus, always enter a number less than the desired number of cycles. Tap on ‘GO TO’, and
enter 2.
Add or delete a step:
1. Add a step—tap to select the step after which a
new step must be inserted.
• Tap on to add a new step.
2. Delete a step—tap to select the step which must
be deleted.
• Tap on to delete an existing step.

Atila Powergene Real-Time PCR Systems User Manual Page 8
3.2.5 Advanced Settings
• By default, the camera is disabled (light gray ).
Tap to enable the camera for the desired steps,
tap on settings . Tap on the switch button next
to CAMERA to turn the camera ON. Tap ‘Modify’
to save changes and ‘Cancel’ to return to the RUN
SETUP screen without saving changes.
• The switch button turns blue when the camera is
ON and gray when the camera is OFF.
• The camera at a particular step is active if the icon
display is dark gray.
Example: All the cameras are active. Example: The camera at STEP 3 is inactive.
• To enable the gradient of the PCR step, tap on .
An Advanced setting overlay appears on the
screen. Tap the switch button next to GRADIENT
to turn the gradient ON (the switch button will turn
blue when gradient is ON). Tap ‘Modify’ to save
changes and ‘Cancel’ to return to the RUN SETUP
screen without saving changes.
Example: Gradient is ON for Step 2 and 3
and OFF for Step 1 and 4. Gradient can be
turned on for all steps, individually.

Atila Powergene Real-Time PCR Systems User Manual Page 9
3.2.6 Saving Run Setup Settings
• Tap to save the current settings to the
protocol. This options enable the user to directly
load and repeat the same experiment again at a
later time.
3.2.7 Run Experiment: Real Time
• Tap to run the saved protocol.
• At any time, the user wishes to stop the
experiment, tap .
• At any time, the user wishes to pause the
experiment, tap . The button changes to .
Tap the green button again to continue the
experiment run.
• The progress bar at the bottom of the screen
displays the percentage of experiment completed
and time remaining.
• The temperature of the Lid ‘LID TEMP.’ and
‘SAMPLE TEMP’ are displayed to the left of the
progress bar.
• To view the amplication curve during the PCR
run, tap . The following screen will appear. Tap
the back button to return to the RUN screen.
3.3 Data Manager
In the data manager screen you can access your run data for
export to a USB.
1. Select the user, the protocol, then the le name.
2. Once the le name is selected, press the forwards
arrow .
3. When the next screen displays, insert a USB drive to the
port in the front.
4. Select your le name, and then “export”. Your le will be
exported to a USB.
The above gure illustrates the
screen display after the user taps .

Copyright © 2020 Atila Biosystems . All rights reserved . The Atila Biosystems logo , Atila Biosystems ®, and Powergene ™are
trademarks of the Company . All other trademarks , service marks and trade names appearing in this brochure are the property of
their respective owners.
www.atilabiosystems.com •[email protected]
3.4 Device Settings
In device settings, the user can customize certain information
on the instrument, as well as look up important technical
information for troubleshooting purposes.
• Device Info—within this screen, the user can see the
factory set serial number. The instrument name is also
visible, and this can be reset to the users choices.
• Date and Time—the user can set the time to reect their
time zone, and adjust the date.
• Network—within the network setting, the user can connect
the machine to Wi-Fi or Ethernet.
• Dye Manager—this tool is designed for users who need to
add a custom dye to their system. Settings include how to
set up the dye plate for calibration.
• Technical Support—this contains information on how to
contact the Atila technical support team.