avantor VWR 76289-386 User manual

Instruction manual
VWR®PCR Workstation HEPA
US Plug: 76289-386
EU Plug: 732-3409
UK Plug: 732-3410
BE-FR Plug: 732-3901
___
Version 03Issue 04/2023

2vwr.com I Instruction manual VWR®PCR Workstation HEPA
Legal address of Manufacturer
Europe
VWR International GmbH,
Hilpertstraße 20a,
64295 Darmstadt, Germany
[phone]
de.vwr.com
UK Importer:
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE17 4XN
uk.vwr.com
United States
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
[phone]
www.vwr.com
Country of Origin : GERMANY

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Table of contents
1Introduction ...........................................................................................................................................................5
2General warning...................................................................................................................................................5
3Safety instructions...............................................................................................................................................5
4Delivery contents ................................................................................................................................................. 7
5Unpacking..............................................................................................................................................................9
6Assembling the PCR Workstation ...................................................................................................................9
7Specifications...................................................................................................................................................... 14
7.1 VWR PCR Workstation ............................................................................................................................ 14
7.2 Power supply............................................................................................................................................... 14
8Connections......................................................................................................................................................... 15
8.1 AC power inlet............................................................................................................................................ 15
8.2 AC power outlets....................................................................................................................................... 16
9Operating the PCR Workstation ....................................................................................................................17
9.1 Use of PCR equipment inside the workstation...................................................................................17
9.2 Operating the overhead UV and white lights ....................................................................................17
9.3 Operating the UV Air Recirculator ........................................................................................................17
9.4 Operating the HEPA/UV System........................................................................................................... 18
9.5 Timer ............................................................................................................................................................. 18
9.6 Control panel description ....................................................................................................................... 19
9.7 Select day and time................................................................................................................................. 20
9.8 Software menu.......................................................................................................................................... 20
9.9 Set up UV Surface time........................................................................................................................... 20
9.10 Set up Auto Decontamination routine ................................................................................................ 21
9.11 Checking and resetting the hours of duty..........................................................................................23
9.12 Settings.........................................................................................................................................................24
10 UV tube intensity................................................................................................................................................26
10.1 Measuring UV tube intensity ..................................................................................................................26
10.2 Bacterial destruction chart.....................................................................................................................26
11 Maintenance .......................................................................................................................................................28
11.1 Care and cleaning.....................................................................................................................................28
11.2 Replacing the UV germicidal or white light tubes ...........................................................................28
11.3 Replacing the UV Air Recirculator UV tube .......................................................................................29
11.4 Replacing the HEPA/UV tube ................................................................................................................29
11.5 Replacing the filter of the UV Air Recirculator..................................................................................29
11.6 Replacing the HEPA and Carbon filter............................................................................................... 30
11.7 Replacing the Pre-filter........................................................................................................................... 30
11.8 Replacing the fuse.................................................................................................................................... 30
12 Ordering Information........................................................................................................................................ 31
13 Technical service................................................................................................................................................ 31
14 Warranty ..............................................................................................................................................................32

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15 Compliance with local laws and regulations.............................................................................................32
16 Disposal Instruction...........................................................................................................................................33

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1Introduction
The VWR PCR Workstation is designed as an ideal environment for the manipulation of DNA and RNA,
especially for the set-up of PCR assays. Contamination can lead to false or misleading results which
costs time and money. The VWR PCR Workstation minimises the risk of contamination. It provides a
'separate room', e.g. for the set-up of PCR reactions. In addition, the HEPA filter (H14 Standard)
provides a barrier against dust, bacteria and mold with a filtration efficiency of 99,995% down to 0.3
microns of particles. The HEPA system provides an ultra-clean particle free working space reducing the
chance of contamination by the air which blows into the PCR chamber.
The high intensity surface UV tubes inactivate DNA as a source of contamination between experiments.
In addition, a UV Air Recirculator is integrated into the workstation system which reduces airborne
contaminants during experiments. UV light is effectively blocked by solid polycarbonate screens to
ensure maximum protection for the user.
The surface of the workstation is constructed of stainless steel, which is very robust and can easily be
cleaned plus has an antimicrobial effect.
The VWR PCR Workstation offers a controlled environment for PCR and RNA applications that
protects your samples and helps to achieve optimal results.
2General warning
The PCR Workstation has been designed for laboratory use and it should not be used at temperature
below 15 °C. If the instrument is at a temperature below 10 °C place it in a warmer room and wait at
least two hours before using it. Before plugging the instrument into a power supply, check that the
voltage and frequency on the label matches the power supply specifications. Before any operation
ensure that the ventilation grids are not obstructed and the door is properly closed.
3Safety instructions
The PCR Workstation is designed with function, reliability, and safety in mind.
Before using the PCR Workstation please read this entire manual and strictly follow the safety
instructions!
Caution: The unit includes shortwave UV, which is a powerful source of UV radiation that will cause
damage to unprotected eyes and skin if directly exposed to the UV lamps. Before operating any unit,
ensure that the PCR Workstation is not damaged, and that instructions for use of this equipment are
followed. A safety shut-off switch automatically turns the UV light off when the front panel is open,
protecting users from UV exposure. The polycarbonate panels are UV-blocking.
Intended use of the PCR Workstation: Working environment for protection of PCR reactions / reaction
set ups and genetic substances against pollution and contamination.

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Safety and protection of the user cannot be ensured if the PCR Workstation is used in any manner that
is not specified in these instructions. Special notice must be taken of the following:
Caution: Dangerous voltage
Ensure that the voltage requirements of the PCR Workstation exactly
match your local AC power supply.
Only use the original AC cable supplied with the PCR Workstation.
Caution: Liquids
Ensure that no liquids can enter the device. Do not place liquids on
top of the PCR Workstation.
Caution: Dangerous explosive material
Do not use reactive or explosive substances in the PCR Workstation.
Caution: Damage
Check for transport damage and completeness of the accessories
upon arrival. If any damage is noticed, contact your distributor or
manufacturer immediately. If you detect any damage of the PCR
Workstation or one of its accessories, do not connect the PCR
Workstation to the AC wall outlet and do not use the device in any
manner!
Caution: Optical radiation
Do not switch on the PCR Workstation if not completely assembled.
See “ASSEMBLING THE PCR WORKSTATION”.
Do not look directly into the UV lamps.

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4Delivery contents
After receipt of the system, please check if the following items are included in the shipment:
1. Base (1x)
2. Rear panel (1x)
3. Side panel (2x)
4. Front panel (1x)
5. Shelf (2x)
6. Top assembly (1x)
7. Ventilation cover (1x)

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8. Screw (31x)
9. Screwdriver (1x)
10. Main cable (1x)
11. Pre-filter (1x)
12. Carbon filter (1x)
13. HEPA filter (1x)
14. Filter holder (1x)

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5Unpacking
−Remove the instrument from its packaging and inspect it for signs of damage. If any are discovered,
inform your supplier immediately
−The instrument must be placed on a stable, level surface and positioned such that air can circulate
freely around the casing
−Ensure your proposed installation site conforms to the environmental conditions for safe operation
−The instrument is designed for indoor use only, temperature range of 10 to 35 °C and humidity of 35
to 70% are recommended
−If the instrument has just been unpacked or has been stored in a cold environment, it should be
allowed to come to room temperature for 2 – 3 hours in the laboratory before switching on. This will
prevent calibration failure because of internal condensation
−The instrument must be connected to the power supply with the power cord supplied.
−It is recommended that users should thoroughly read this manual prior to use
−Contact your supplier if you experience any difficulties with this instrument
6Assembling the PCR Workstation
The PCR Workstation should be assembled by two people, directly at the site of operation. We
recommend the wearing of safety gloves for hand protection.
Step 1
Put the base on an even surface with the rubber
feet facing down and the sensor cable outlet
facing to the front. Lay the back panel onto the
base with the power outlets facing down, and
the power input and the type label facing to the
right.
Connect both sensor cables.
Base

Back panel


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Slide the connection assembly into the cavity of
the back panel.
Be careful not to damage the blue sensor
cables. Warranty for this type of damage
is excluded.
Slot the back panel onto the base . The
connecting link of the base must fit in the back
panel . Take care not to squeeze the sensor
cable.
Connect both parts by screwing 3 screws into the back side. Use the screw driver to do this. Turn
the parts around 90° on the site of operation and incline both screwed parts to the back part of the
back panel . Screw both panels with 2 screws from underneath. Tilt back the panels in starting
position with the back panel facing backwards.

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Step 2
Remove the protective foil from the side panels.
Insert the side panels into the guiding rails at
the base and push up against the back
panel within the guiding rails at both sides.
Carefully screw the side panels with 3 screws
each in the base and the back panel ,
respectively. Use the supplied screws and the
screwdriver .
Step 3
Put the shelves into the shelf brackets at the
back panel .
Remove the protective foil from the front panel.
Put the front panel onto the side panels .
Take care that the screw holes of the front panel
match the threaded holes in the side panels .
Hold the front panel in place. Screw the front
panel to both side panels using 3 screws 
each. Use the screwdriver .
Step 4
Turn the assembled parts around 180°.
Remove the small UV tube at the back of the top
assembly.
Put the top assembly onto the panels. Take care
not to squeeze the cables protruding from the
back panel. Screw the top assembly to the back
panel at two positions. Use the screws and
screwdriver supplied.
Connect the sensor cables from the back panel 
and the top assembly . Put the power plug of the
back panel into the power outlet of the top
assembly .
Back panel

Base

Side panel

Side panel

Shelves

Front panel

Side panel

Side panel

Top assembly

Back panel


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Step 5
Insert the UV tube back in place.
Cover the service access of the top assembly 
with the ventilation cover . Screw both parts
together with 6 screws . Use the screw-driver .
Turn the system around 180° and place it at the
site of operation as needed.
Step 6
Unpack the HEPA filter from foil.
To ensure the HEPA filter efficiency please follow the
installation steps below.
•Unscrew the six star grip screws until their end is
flush with the end of the threaded holes.
•Insert the HEPA filter into the filter holder. Pay
attention to the correct alignment (air flow).
•Place the activated carbon filter with the
air outlet side facing upwards and
grease the seal generously with
petroleum jelly.
Ventilation cover

Top assembly


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•Place the activated carbon filter with the greased sealing side on the HEPA filter. Pay attention
to the correct alignment (air flow). Grease the now above seal generously with petroleum jelly.
•Insert the air filter holder prepared in this
way into the hood and press it in firmly.
•Screw in the six star grip screws evenly until
the gap between the HEPA filter and the
filter holder is about 7 to 10 mm (0,25 – 0,4”)
in size. Then the seals are pressed sufficiently
strongly.
Finally connect the system to the power supply by plugging the power plug from the back panel 
into the local power outlet.
The system is now ready for operation now.

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7Specifications
7.1 VWR PCR Workstation
Light sources integrated into the top assembly:
•2x 25 W 254 nm UV light tubes – for effective surface decontamination
•1x 8 W 254 nm UV light tube – for decontamination of air during work in UV Air
Recirculator
•1x 8 W 254 nm UV light tube – for decontamination of air during work with HEPA Filter
•1x 15 W white light tube – for a bright work surface
Features:
•Four power outlets
•Two shelves made from stainless steel
•Electronic UV timer
•Working area and back panel made from stainless steel
•Front and side panels made from polycarbonate (Makrolon®) that blocks wavelengths
below 400 nm
•Three-stage filter system with Pre-filter, Carbon filter and HEPA filter
•Hole for HEPA filter certification (please check Chapter DOP test).
LxWxH: 33 x 29.5 x13 in ; 750x620x795 mm
Work Surface (W×D) : 715×540 mm
Weight: 114.6lbs ; 52 Kg
Pollution rate: 2
Environmental temperature range: +10 to +30 °C
Maximum relative humidity: 70%
Maximum height above sea level: 2000 m
Noise level (at 0,5m/sec): 43 dB (A)
Noise lxel (max): 60 dB (A)
Circulation rate fan: 56 m3/h (UV Air Recirculator)
50 – 250 m³/h (HEPA Filter)
7.2 Power supply
EU/FR-BE/UK Version : 220 - 240 V AC, 50/60 Hz, max. 1650 VA
US Version : 120 V, 60 Hz, max. 900 VA
Fuse: 10 AT, 5 x 20 mm
For indoor use, only!

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8Connections
8.1 AC power inlet
Before first use, ensure that the voltage requirements of the PCR Workstation exactly match your local
AC power supply. Connect the PCR Workstation to the AC power supply only by the AC power cord
delivered with your PCR Workstation. Only connect the PCR Workstation to electric power systems
with an earth conductor. The AC power inlet and the AC power switch are located at the rear of the
workstation. The AC power switch serves as a disconnection of the AC power supply from the device.
The accessibility of the AC power switch must be not hindered or obscured in any way.
The electric fuse is placed at the back of the device between the power switch and the AC power inlet.
After pulling off the AC power cord and pushing upwards a safety catch, the fuse can be changed. Use
only a fuse with correct values (see "SPECIFICATIONS"). Indications about the fuse type are also
located at the back of the device.
If a service is required, contact your local distributor or the manufacturer (see "TECHNICAL SERVICE
AND ORDER INFORMATION").
The serial number of the PCR Workstation is located at the rear of the instrument. The replacement of
single components (except polycarbonate panels, UV tubes and the UV Air Recirculator fuse and filter)
must not be done by the user but exclusively by authorised specialists using original replacement parts.
Therefore, the device must be sent to the manufacturer. Unauthorised work on the device voids the
warranty.
The surface of the device should be decontaminated with a lint-free cloth soaked with 70% ethanol
before sending. Please fill in and sign the decontamination certificate and send it to the manufacturer
together with the device.
For safety reasons the device must be switched off and the mains plug pulled before cleaning is
performed!
AC power inlet
AC power switch
Fuse

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8.2 AC power outlets
The 4 AC power outlets serve as power supplies for devices used inside the PCR chamber.
PCR workstation version
reference
Plug Type
Sockets type
Voltage
Grounded
North American
76289-386
Type B (NEMA 5-15)
Type B (NEMA 5-15)
120 V
Yes
Europe
732-3409
Type F, CEE 7/4
Type F, CEE 7/4
230 V
Yes
UK
732-3410
Type G, BS 1363
Type G, BS 1363
230 V
Yes
BE-FR
732-3901
Type E, CEE 7/6
Type E, CEE 7/6
230 V
Yes
For PCR workstation 732-3409 :
When attaching appliance with Type E CEE 7/6 (French) plug, use correct socket adapter!
When attaching appliance with Type J SN 441011 (Swiss) plug, use correct socket adapter!
The sum of the maximum power ratings of devices connected to these power outlets must
not exceed the values specified for the PCR Workstation.
See chapter "OPERATING THE PCR WORKSTATION ", for further information.
4x AC power outlet

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9Operating the PCR Workstation
9.1 Use of PCR equipment inside the workstation
Shelves are provided for the placing of small tools for storage and decontamination.
Power outlets allow use of shakers, rockers and other equipment for PCR experiments inside the
PCR chamber. The equipment can be decontaminated between experiments. To operate
equipment within the chamber, plug the equipment into the power plug. Specific power plugs are
installed as shown in the illustration "AC power outlets".
Timing of UV decontamination can be programmed for time-controlled every day
decontamination.
NOTE: When operating, equipment plugged into the outlets, combined amp total should not exceed
6 amps.
NOTE: Crazing is a normal process for polycarbonate panels exposed to UV light. Crazing will occur
over a period of time. Reduce crazing by keeping exposure to UV to a minimum. Crazing may occur
within the warranty period and is regarded as normal wear and tear not covered by the warranty.
Polycarbonate panels are available as spare parts and can be replaced.
9.2 Operating the overhead UV and white lights
The PCR Workstation provides an automated process for eliminating contamination by using the
decontaminating properties of shortwave 254 nm ultraviolet light. The UV light is typically operated
when no samples are inside the chamber. The chamber is decontaminated and DNA as a possible
contamination is inactivated.
The overhead white light can remain lit at any time throughout an experiment or a decontamination
process. The fluorescent white light provides a bright light within the PCR Workstation.
9.3 Operating the UV Air Recirculator
The VWR PCR Workstation includes a built-in UV Air Recirculator for elimination of airborne microbes
during experiments.
NOTE: The UV Air Recirculator can operate while the front panel is open.
The UV Air Recirculator circulates airflow into and out of the chamber. The air is sucked into the UV Air
Recirculator in the top of the chamber, the air outlets are at the bottom of the back panel. The drawing
indicates the location of the air vents.
NOTE: Equipment in front of the air vents will block airflow.

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9.4 Operating the HEPA/UV System
Push the HEPA button to operate the HEPA/UV filter system. The airflow fan setting can be
adjusted in four steps (0.3m/s, 0.5m/s (Factory Default), 0.8m/s, 1.2m/s) by pressing the or button
in the start screen. The UV light starts first followed by the airflow fan, to avoid contaminations from
inside of the top assembly.
The filter system consists of:
•Pre-filter to preserve the life of other filters by capturing large dust particles
•Carbon filter to remove ozone and gases
•HEPA filter (H14 Standard) provides a barrier against dust, bacteria and mold.
9.5 Timer
The default setting is 30 minutes adjustable at 5 minutes’ increments down to a minimum of 5 minutes.
Note: A magnetic sensor is built into the bottom of the front panel. When the front panel is opened,
the ultraviolet light will automatically shut off!
Note: Do not attempt to perform PCR procedures with the germicidal lamp on. The germicidal lamp is
used to decontaminate the chamber between experiments.

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9.6 Control panel description
To scroll or increase selected value
To scroll or decrease selected value
Enables to select and change the displayed program settings. Several clicks to
jump from value to value.
Enables to open and close the software menu
Switch white light on and off
Switch UV light on and off
It automatically activates the programme to decontaminate the workspace.
The default time for the decontamination process is 30 minutes.
The display will show the remaining time of the decontamination programme.
Remaining time could be change with the arrow buttons.
Switch Recirculator on and off
Switch HEPA/UV system on and off

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9.7 Select day and time
Press from the home screen to change
day and time. Pressing the or button will
modify the selected value.
Press to go to the next values: hours,
minutes and safe.
9.8 Software menu
Press to open the software menu.
Pressing the

or

button will go through the
menu items. An arrow is displayed near the
selected item.
The items are:
- UV Surface
- Auto Decontamination
- Duty hours
- Settings
−
9.9 Set up UV Surface time
Select “UV Surface” and click on
.
The time
starts flashing. Pressing the or

button will
modify the time between 30 min and 5 min in
steps of 5 minutes. To store the selected value
press .