Esco Labculture PLUS LP2-4S Series User manual

Labculture Plus Class II Biological Safety Cabinet,
Model LP2-4S_.
Class II Biological Safety Cabinets
The Safety Solution for Life Science Laboratories

Biological Safety Cabinets • Class II Biological Safety Cabinets
2
Each cabinet is KI-Discus type tested for performance integrity.
Available in 1.2 and 1.8 meter models (4' and 6').
Shown with optional telescoping stand.
2
Main Features
Reduced height profile and noise levels compared
with Esco Labculture.
The front sash is motorized for convenient one
touch operation.
Sentinel™Gold microprocessor monitors airflow in
real-time via temperature compensated sensors and
conserves UV lamp life with unique timer function.
The Accuflow microprocessor based speed controller
maintains constant, stable airflow despite building
supply voltage fluctuations.
The angled front, narrow profile front grille, raised
armrest and frameless sash creates an ergonomic
work environment.
ULPA filters (per IEST-RP-CC-001.3) are tested
to a typical efficiency of >99.999% for 0.1 to
0.3 micron particles, better than HEPA filters.
Unique Esco Dynamic Chamber™plenum.
Negative pressure plenum surrounds
contaminated positive pressure plenum; no
fabric bags are used.
Include multi-piece tray components which lift
and remove to provide easy access encourage
surface decontamination and can be sterilized
inside autoclave.
Esco antimicrobial coating on all
painted surfaces minimizes contamination.

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Containment and Protection
• Inow of room air enters the front air
grille to establish operator protection;
room air does not enter the work zone,
preventing product contamination.
• Auto-purge holes located at the front
side walls eliminate eddy currents and
dead air pockets in the critical area
behind the sash window.
• The ULPA downow (supply) lter is
tilted proportional to the cabinet front
angle to direct more air forward to the
front air grille.
• The inow velocity, downow velocity,
air ow path, and intake geometry are
precision tuned and tested to create an
optimum air curtain on the front aperture;
this curtain maintains personnel and
product protection even in the unlikely
event of a severe inow or downow
imbalance that would compromise
protection in a conventional cabinet.
Integrated Filtration System
Independent supply and exhaust filters
provide 99.999% typical efficiency for
particle sizes of 0.1 to 0.3 recommended
practice for ULPA performance (USA), and
EN 1822 for H14 performance (EU).
• ULPA filters (per IEST-RP-CC001.3),
are tested to a typical efficiency of
>99.999% for 0.1 to 0.3 micron particles;
these provide better ltration capability
than conventional H13 HEPA lters that
have a typical efciency of >99.99% for
0.3 micron particles.
• Modern separator-less mini-pleat lter
construction maximizes the lter surface
area to extend lter life and eliminate
possible lter media damage by thin
and sharp aluminum separators used in
conventional HEPA lter construction.
• The lter frame and media is constructed
in accordance with EN1822 requirements
for re retardant properties.
• The supply ULPA lter provides ISO Class
3 (per ISO14644.1) clean air to the work
surface in a gentle vertical laminar ow
for product protection.
• The exhaust ULPA lter traps biohazard
particles acquired from the work surface
before air is exhausted to the room,
offering personnel and environmental
protection.
• The exhaust lter media is protected
from mechanical damage by an
integrated metal screen guard, which is
absent from conventional HEPA lters.
Front Sash Assembly
• Motorized front sash allows ngertip
operation.
• Integrated sash proximity contacts
sense proper sash position, serve as an
interlock for the UV lamp, and activate
an alarm if the sash is improperly
positioned.
• The magnetic switch eliminates the
chance of wear and tear typical of a
mechanical switch.
• The back of the sash can be easily
cleaned by removing the sash track
cover and swinging up the sash glass.
Blower Efficiency
The Labculture Plus blower system is designed
for high performance operation, maxi-
mum energy efficiency and minimal
maintenance.
• The external rotor motor design allows
for optimum cooling of the motor
during extended operations and extends
the motor bearing life.
• The permanently lubricated direct-drive
external rotor motor/blower reduces
operating costs.
• Built-in RFI and electrical noise lters
eliminate interference with adjacent
instrumentation.
• An integral blower hour meter tracks
operating life for predictive maintenance
planning.
• To prevent fan damage, a paper-catch
grille traps papers or towels that may
drop down on the drain pan, preventing
them from being pulled into the column
by fan suction.
Sentinel Microprocessor Control,
Alarm, Monitoring System
The Esco Sentinel™ microprocessor-based
control system supervises operation of all
cabinet functions.
• The control panel is located on the
center of the cabinet, and angled down
for easy access by the operator.
Mini-pleat Separatorless Filter (left) vs.
Conventional Aluminium Separator Filter (right)
Esco cabinets use Swedish Camfil Farr® mini-pleat
filters without aluminum separators to increase filter
efficiency, minimize the chance of leakage, and to
prolong filter life. Filters include a lightweight
aluminum frame for structural stability and
elimination of swelling common to conventional
wood frames.
Esco ULPA Filter Efficiency
Esco cabinets use ULPA filters
(per IEST-RP-CC001.3) instead
of conventional HEPA filters
commonly found in biological
safety cabinets. While HEPA filters
offer 99.99% typical efficiency
at 0.3 micron level, ULPA filters
provide 99.999% typical efficiency
for particle sizes of 0.1 to 0.3
micron level.
Typical Penetration
Operator, Product and Environmental Protection
The Esco Labculture Plus Class II Biosafety Cabinet
provides operator, product and environmental protection
against Biosafety Levels 1, 2 and 3. This cabinet can be
used for handling Biohazard Level 4, provided that the
operator wears positive pressure suit.

Biological Safety Cabinets • Class II Biological Safety Cabinets
44
Ambient air is pulled through the
perforations located towards the work
zone front to prevent contamination of
the work surface and work product. The
inflow does not mix with the clean air
within the cabinet work zone. Inflow air
travels through a return path toward the
common air plenum (blower plenum) at
the top of the cabinet.
Approximately 32% of the air in the
common plenum is exhausted through
the ULPA filter to the room. The
remaining 68% of the air is passed
through the downflow ULPA filter and
into the work area as a vertical laminar
flow air stream bathing the work surface
in clean air.
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
Near the work surface, the downflow
air stream splits with a portion moving
toward the front air grille, and the
remainder moving to the rear air grille.
A small portion of the ULPA filtered
downflow enters the intake perforations
at the side capture zones at a higher
velocity (small blue arrows).
A combination of inflow and downflow
air streams forms an air barrier that
prevents contaminated room air from
entering the work zone, and prevents
work surface emissions from escaping
the work zone.
Air returns to the common air plenum
where the 32% exhaust and 68%
recirculation process is continued.
ULPA-filtered air
Unfiltered / potentially contaminated air
Room air / Inflow air
Cabinet Filtration System
Side capture zones
Dynamic air barrier, inflow and forward-directed downflow air converge
• Continuous monitoring of cabinet
airow is displayed on a bright, easy-to-
read LCD panel.
• Two integrated, temperature-compen-
sated, true airflow velocity sensors
provides an accurate airow reading
despite room temperature uctuation.
• All electronic parts are contained inside
a plug-and-play module that permits
easy exchange if required.
• Microprocessor software updates are
available from Esco for download via
the Internet.
Sentinel functions are factory set to default
to ON or OFF, depending on worldwide
destination and local preferences. Default
settings can be user activated through the
touchpad data entry access.
• Automatic start-up sequence will pre-
pare the cabinet for normal operation
and advise when safe conditions are
established.
• An administrator controlled PIN
(Personal Identication Number) can be
set to restrict access to main menu.
• The airow alarm can be activated or
deactivated depending on user prefer-
ence and nature of the work.
Consult your Esco Operating Manual
or contact your Sales Representative
for information on user-preference
programming capabilities built into the
Sentinel microprocessor platform.
Cabinet Construction
Robust construction and enhanced safety
features qualify the cabinet for the most
demanding laboratory applications. The
cabinet is fully assembled and ready to install
and operate when shipped.
• The interior work area is formed from a
single piece of stainless-steel with large
radius corners to simplify cleaning.
• The cabinet work zone has no welded
joints to collect contaminants or rust.
• All stainless steel work surfaces are
accessible for cleaning.
• Tray components lift and remove to
provide easy access and encourage
surface decontamination.
• A recessed central area and stainless
steel drain pan channels spills and
prevent liquids from entering the lower
ltration and blower systems.
• The drain pan is flush with the side
walls to eliminate concealed or hard-
to-clean spaces.
• There are no screws in on the front or
sides to trap contaminants or complicate
cleaning.
• Optional service ttings are offset for
easier access.
• External surfaces are coated with Esco
Isocide™ antimicrobial coating to protect
against surface contamination and
inhibit bacterial growth. Isocide
eliminates 99.9% of surface bacteria
within 24 hours of exposure.
Comfortable Ergonomic Design
The LP2 cabinet is engineered for comfort,
utility value and safety.
• The 5° angled viewing window and
narrow profile front grille improves
reach into the work area.
• The instant-start 5000k fluorescent
lamp operates on an electronic ballast
to reduce heat, improve comfort and
conserve energy.
Esco Centrifugal Fan with External Rotor Motor (left) vs.
Conventional Fan with Standard Motor (right)
Esco cabinets use German made ebm-papst®
permanently lubricated, centrifugal motor/
blowers with external rotor designs.
Integrated blades narrow the profile and
eliminate need for a motor shaft.
Motors are selected for energy efficiency,
compact design, and flat profile. The completely
integrated assembly optimizes motor cooling.
All rotating parts are unitized and balanced
for smooth, quiet, vibration-free operation.

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Sentinel Microprocessor Control System, Programmable
Touchpad data entry buttons permit control
settings and access to diagnostics, default
settings and hierarchical menus.
Color coded indicator lamps display green for primary
function (fan operation); blue for secondary function
(fluorescent lights and electrical outlet); and orange
for caution (UV lamp ON).
When programmed ON • the start-up sequence conrms status with Air Safe and local time display.
• the Personal Identication Number (PIN) access restricts unauthorized adjustments.
• an airow alarm warns of deviations from normal velocities.
• The lamp delivers uniform lighting to
the work surface for greater comfort,
reduced glare and improved productivity;
see Technical Specications.
• The front armrest is raised above the
workzone to improve comfort and to
ensure that the operator’s arms do not
block the forward airow perforations.
• The optional adjustable support stand
provides work surface height control.
• The frameless sash eliminates opera-
tor's line of sight blockage.
• A generous sash opening allows for
easier access into the work zone, pro-
vides ample room for transferring of
small equipment.
• The sliding window can be fully opened
to insert and remove larger instrumen-
tation and equipment.
Electrical Safety and Certification
All components meet or exceed applicable
safety requirements.
• Each cabinet is individually tested for
electrical safety at factory.
• Documentation specic to each cabinet
serial number is maintained on le.
• Certied to major world standards for
biological safety cabinets, including the
EN12469 (refer to Standards Compliance
table on this page for the full list).
• Contact Esco or your Sales Representative
for site preparation information; see
Electrical Specications.
Warranty
The Labculture Plus cabinet is warranted
for 3 years excluding consumable parts
and accessories.
• Each cabinet is shipped with a compre-
hensive user’s manual complete with a
report documenting all test procedures.
• Additional IQ/OQ/PQ documentation
is available upon request.
• Contact your local Sales Representative
for specic warranty details or docu-
mentation requests.
Service fixtures are offset for easier reach. Standard cabinets include two fixture provisions on each sidewall
(one provision on each sidewall for 0.9 meter/3 ft. cabinet). Electrical outlets are mounted below service
fixtures to minimize obstructions.
Helpful for certifiers, the hinged maintenance assembly opens to a fixed position on integrated, gas spring
struts providing front service access.
All key components, with the exception of the blower/motor assembly, are mounted outside the air stream
and away from contaminated air to permit service without decontamination. These include fluorescent lamps,
UV lamp, electrical harnesses, electronic boards and microprocessor control.
Panels enclosing potentially hazardous areas or components such as microbiological contamination or
electrical shock are color-coded red to warn service technician.
The telescoping Dynamic Chamber™plenum minimizes physical lifting and accelerates filter change when required.
Work area containment is maintained even when removable components are lifted out for cleaning.
The drain pan is flush with the side walls to elliminate consealed or hard-to-clean spaces.
The lower drain trough is a single-piece fabrication with wide open angles and a channel to direct spills to
the drain.
Robust Cabinet Construction and Enhanced Safety Features
Programmable automatic UV light timer
simplifies operation, enhances contamination
control, extends UV lamp life and saves
energy.
A graphical interface indicates cabinet
performance.
Digital read-out with alpha-numeric
display indicates all input, status and alarm
functions.
All functions can be user
activated through touch-pad
programming access; see
Operations Manual.