Esco Labculture PLUS LP2-4S Series User manual

Labculture Plus Class II Biological Safety Cabinet,
Model LP2-4S_.
Class II Biological Safety Cabinets
The Safety Solution for Life Science Laboratories

Biological Safety Cabinets • Class II Biological Safety Cabinets
2
Each cabinet is KI-Discus type tested for performance integrity.
Available in 1.2 and 1.8 meter models (4' and 6').
Shown with optional telescoping stand.
2
Main Features
Reduced height profile and noise levels compared
with Esco Labculture.
The front sash is motorized for convenient one
touch operation.
Sentinel™Gold microprocessor monitors airflow in
real-time via temperature compensated sensors and
conserves UV lamp life with unique timer function.
The Accuflow microprocessor based speed controller
maintains constant, stable airflow despite building
supply voltage fluctuations.
The angled front, narrow profile front grille, raised
armrest and frameless sash creates an ergonomic
work environment.
ULPA filters (per IEST-RP-CC-001.3) are tested
to a typical efficiency of >99.999% for 0.1 to
0.3 micron particles, better than HEPA filters.
Unique Esco Dynamic Chamber™plenum.
Negative pressure plenum surrounds
contaminated positive pressure plenum; no
fabric bags are used.
Include multi-piece tray components which lift
and remove to provide easy access encourage
surface decontamination and can be sterilized
inside autoclave.
Esco antimicrobial coating on all
painted surfaces minimizes contamination.

33
Containment and Protection
• Inow of room air enters the front air
grille to establish operator protection;
room air does not enter the work zone,
preventing product contamination.
• Auto-purge holes located at the front
side walls eliminate eddy currents and
dead air pockets in the critical area
behind the sash window.
• The ULPA downow (supply) lter is
tilted proportional to the cabinet front
angle to direct more air forward to the
front air grille.
• The inow velocity, downow velocity,
air ow path, and intake geometry are
precision tuned and tested to create an
optimum air curtain on the front aperture;
this curtain maintains personnel and
product protection even in the unlikely
event of a severe inow or downow
imbalance that would compromise
protection in a conventional cabinet.
Integrated Filtration System
Independent supply and exhaust filters
provide 99.999% typical efficiency for
particle sizes of 0.1 to 0.3 recommended
practice for ULPA performance (USA), and
EN 1822 for H14 performance (EU).
• ULPA filters (per IEST-RP-CC001.3),
are tested to a typical efficiency of
>99.999% for 0.1 to 0.3 micron particles;
these provide better ltration capability
than conventional H13 HEPA lters that
have a typical efciency of >99.99% for
0.3 micron particles.
• Modern separator-less mini-pleat lter
construction maximizes the lter surface
area to extend lter life and eliminate
possible lter media damage by thin
and sharp aluminum separators used in
conventional HEPA lter construction.
• The lter frame and media is constructed
in accordance with EN1822 requirements
for re retardant properties.
• The supply ULPA lter provides ISO Class
3 (per ISO14644.1) clean air to the work
surface in a gentle vertical laminar ow
for product protection.
• The exhaust ULPA lter traps biohazard
particles acquired from the work surface
before air is exhausted to the room,
offering personnel and environmental
protection.
• The exhaust lter media is protected
from mechanical damage by an
integrated metal screen guard, which is
absent from conventional HEPA lters.
Front Sash Assembly
• Motorized front sash allows ngertip
operation.
• Integrated sash proximity contacts
sense proper sash position, serve as an
interlock for the UV lamp, and activate
an alarm if the sash is improperly
positioned.
• The magnetic switch eliminates the
chance of wear and tear typical of a
mechanical switch.
• The back of the sash can be easily
cleaned by removing the sash track
cover and swinging up the sash glass.
Blower Efficiency
The Labculture Plus blower system is designed
for high performance operation, maxi-
mum energy efficiency and minimal
maintenance.
• The external rotor motor design allows
for optimum cooling of the motor
during extended operations and extends
the motor bearing life.
• The permanently lubricated direct-drive
external rotor motor/blower reduces
operating costs.
• Built-in RFI and electrical noise lters
eliminate interference with adjacent
instrumentation.
• An integral blower hour meter tracks
operating life for predictive maintenance
planning.
• To prevent fan damage, a paper-catch
grille traps papers or towels that may
drop down on the drain pan, preventing
them from being pulled into the column
by fan suction.
Sentinel Microprocessor Control,
Alarm, Monitoring System
The Esco Sentinel™ microprocessor-based
control system supervises operation of all
cabinet functions.
• The control panel is located on the
center of the cabinet, and angled down
for easy access by the operator.
Mini-pleat Separatorless Filter (left) vs.
Conventional Aluminium Separator Filter (right)
Esco cabinets use Swedish Camfil Farr® mini-pleat
filters without aluminum separators to increase filter
efficiency, minimize the chance of leakage, and to
prolong filter life. Filters include a lightweight
aluminum frame for structural stability and
elimination of swelling common to conventional
wood frames.
Esco ULPA Filter Efficiency
Esco cabinets use ULPA filters
(per IEST-RP-CC001.3) instead
of conventional HEPA filters
commonly found in biological
safety cabinets. While HEPA filters
offer 99.99% typical efficiency
at 0.3 micron level, ULPA filters
provide 99.999% typical efficiency
for particle sizes of 0.1 to 0.3
micron level.
Typical Penetration
Operator, Product and Environmental Protection
The Esco Labculture Plus Class II Biosafety Cabinet
provides operator, product and environmental protection
against Biosafety Levels 1, 2 and 3. This cabinet can be
used for handling Biohazard Level 4, provided that the
operator wears positive pressure suit.

Biological Safety Cabinets • Class II Biological Safety Cabinets
44
Ambient air is pulled through the
perforations located towards the work
zone front to prevent contamination of
the work surface and work product. The
inflow does not mix with the clean air
within the cabinet work zone. Inflow air
travels through a return path toward the
common air plenum (blower plenum) at
the top of the cabinet.
Approximately 32% of the air in the
common plenum is exhausted through
the ULPA filter to the room. The
remaining 68% of the air is passed
through the downflow ULPA filter and
into the work area as a vertical laminar
flow air stream bathing the work surface
in clean air.
The uniform, non-turbulent air stream
protects against cross contamination
within and throughout the work area.
Near the work surface, the downflow
air stream splits with a portion moving
toward the front air grille, and the
remainder moving to the rear air grille.
A small portion of the ULPA filtered
downflow enters the intake perforations
at the side capture zones at a higher
velocity (small blue arrows).
A combination of inflow and downflow
air streams forms an air barrier that
prevents contaminated room air from
entering the work zone, and prevents
work surface emissions from escaping
the work zone.
Air returns to the common air plenum
where the 32% exhaust and 68%
recirculation process is continued.
ULPA-filtered air
Unfiltered / potentially contaminated air
Room air / Inflow air
Cabinet Filtration System
Side capture zones
Dynamic air barrier, inflow and forward-directed downflow air converge
• Continuous monitoring of cabinet
airow is displayed on a bright, easy-to-
read LCD panel.
• Two integrated, temperature-compen-
sated, true airflow velocity sensors
provides an accurate airow reading
despite room temperature uctuation.
• All electronic parts are contained inside
a plug-and-play module that permits
easy exchange if required.
• Microprocessor software updates are
available from Esco for download via
the Internet.
Sentinel functions are factory set to default
to ON or OFF, depending on worldwide
destination and local preferences. Default
settings can be user activated through the
touchpad data entry access.
• Automatic start-up sequence will pre-
pare the cabinet for normal operation
and advise when safe conditions are
established.
• An administrator controlled PIN
(Personal Identication Number) can be
set to restrict access to main menu.
• The airow alarm can be activated or
deactivated depending on user prefer-
ence and nature of the work.
Consult your Esco Operating Manual
or contact your Sales Representative
for information on user-preference
programming capabilities built into the
Sentinel microprocessor platform.
Cabinet Construction
Robust construction and enhanced safety
features qualify the cabinet for the most
demanding laboratory applications. The
cabinet is fully assembled and ready to install
and operate when shipped.
• The interior work area is formed from a
single piece of stainless-steel with large
radius corners to simplify cleaning.
• The cabinet work zone has no welded
joints to collect contaminants or rust.
• All stainless steel work surfaces are
accessible for cleaning.
• Tray components lift and remove to
provide easy access and encourage
surface decontamination.
• A recessed central area and stainless
steel drain pan channels spills and
prevent liquids from entering the lower
ltration and blower systems.
• The drain pan is flush with the side
walls to eliminate concealed or hard-
to-clean spaces.
• There are no screws in on the front or
sides to trap contaminants or complicate
cleaning.
• Optional service ttings are offset for
easier access.
• External surfaces are coated with Esco
Isocide™ antimicrobial coating to protect
against surface contamination and
inhibit bacterial growth. Isocide
eliminates 99.9% of surface bacteria
within 24 hours of exposure.
Comfortable Ergonomic Design
The LP2 cabinet is engineered for comfort,
utility value and safety.
• The 5° angled viewing window and
narrow profile front grille improves
reach into the work area.
• The instant-start 5000k fluorescent
lamp operates on an electronic ballast
to reduce heat, improve comfort and
conserve energy.
Esco Centrifugal Fan with External Rotor Motor (left) vs.
Conventional Fan with Standard Motor (right)
Esco cabinets use German made ebm-papst®
permanently lubricated, centrifugal motor/
blowers with external rotor designs.
Integrated blades narrow the profile and
eliminate need for a motor shaft.
Motors are selected for energy efficiency,
compact design, and flat profile. The completely
integrated assembly optimizes motor cooling.
All rotating parts are unitized and balanced
for smooth, quiet, vibration-free operation.

55
Sentinel Microprocessor Control System, Programmable
Touchpad data entry buttons permit control
settings and access to diagnostics, default
settings and hierarchical menus.
Color coded indicator lamps display green for primary
function (fan operation); blue for secondary function
(fluorescent lights and electrical outlet); and orange
for caution (UV lamp ON).
When programmed ON • the start-up sequence conrms status with Air Safe and local time display.
• the Personal Identication Number (PIN) access restricts unauthorized adjustments.
• an airow alarm warns of deviations from normal velocities.
• The lamp delivers uniform lighting to
the work surface for greater comfort,
reduced glare and improved productivity;
see Technical Specications.
• The front armrest is raised above the
workzone to improve comfort and to
ensure that the operator’s arms do not
block the forward airow perforations.
• The optional adjustable support stand
provides work surface height control.
• The frameless sash eliminates opera-
tor's line of sight blockage.
• A generous sash opening allows for
easier access into the work zone, pro-
vides ample room for transferring of
small equipment.
• The sliding window can be fully opened
to insert and remove larger instrumen-
tation and equipment.
Electrical Safety and Certification
All components meet or exceed applicable
safety requirements.
• Each cabinet is individually tested for
electrical safety at factory.
• Documentation specic to each cabinet
serial number is maintained on le.
• Certied to major world standards for
biological safety cabinets, including the
EN12469 (refer to Standards Compliance
table on this page for the full list).
• Contact Esco or your Sales Representative
for site preparation information; see
Electrical Specications.
Warranty
The Labculture Plus cabinet is warranted
for 3 years excluding consumable parts
and accessories.
• Each cabinet is shipped with a compre-
hensive user’s manual complete with a
report documenting all test procedures.
• Additional IQ/OQ/PQ documentation
is available upon request.
• Contact your local Sales Representative
for specic warranty details or docu-
mentation requests.
Service fixtures are offset for easier reach. Standard cabinets include two fixture provisions on each sidewall
(one provision on each sidewall for 0.9 meter/3 ft. cabinet). Electrical outlets are mounted below service
fixtures to minimize obstructions.
Helpful for certifiers, the hinged maintenance assembly opens to a fixed position on integrated, gas spring
struts providing front service access.
All key components, with the exception of the blower/motor assembly, are mounted outside the air stream
and away from contaminated air to permit service without decontamination. These include fluorescent lamps,
UV lamp, electrical harnesses, electronic boards and microprocessor control.
Panels enclosing potentially hazardous areas or components such as microbiological contamination or
electrical shock are color-coded red to warn service technician.
The telescoping Dynamic Chamber™plenum minimizes physical lifting and accelerates filter change when required.
Work area containment is maintained even when removable components are lifted out for cleaning.
The drain pan is flush with the side walls to elliminate consealed or hard-to-clean spaces.
The lower drain trough is a single-piece fabrication with wide open angles and a channel to direct spills to
the drain.
Robust Cabinet Construction and Enhanced Safety Features
Programmable automatic UV light timer
simplifies operation, enhances contamination
control, extends UV lamp life and saves
energy.
A graphical interface indicates cabinet
performance.
Digital read-out with alpha-numeric
display indicates all input, status and alarm
functions.
All functions can be user
activated through touch-pad
programming access; see
Operations Manual.

Biological Safety Cabinets • Class II Biological Safety Cabinets
6
Input Voltage
Blower Speed
Input Voltage
Blower Speed
The Esco Accuflow™ microprocessor speed controller maintains
steady blower motor speed despite building voltage fluctuations,
thereby assuring constant face velocity and downflowfor optimum
safety, containment and protection.
The Esco Accuflow™microprocessor speed controller maintains steady motor/blower speed despite
building voltage fluctuations, thereby assuring constant face velocity and downflow for optimum safety,
containment and protection.
Esco Accuflow Microprocessor Speed Controller
6
Accessories and Options
Esco offers a variety of options and acces-
sories to meet local applications. Contact
Esco or your local Sales Representative for
ordering information.
Single Piece Work Tray
Available for applications where a sumped
work surface is desired to contain spillage.
Must be specied when ordering.
Support Stands
• Fixed height, available 711 mm (28")
or 864 mm (34")
- With leveling feet (±38.1 mm /1.5")
- With casters
• Adjustable height, hydraulic range
711 mm to 864 mm (28" to 34")
- With leveling feet
- With casters
• Telescoping height stand for leveling
feet, nominal range 660 mm or
960 mm (26" or 37.8")
• Telescoping height stand for casters,
nominal range 660 mm or 880 mm
(26" or 34.6")
- Adjustable in 25.4 mm (1") increments
• Cradle stand, electrical hydraulic,
innitely adjustable, with casters
- Elevates to seating or standing
work surface height.
- When lowered permits movement
through standard doorway.
Note: Increases exterior dimensions.
Electrical Outlets and Utility Fixtures
• Electrical outlet, ground fault,
North America
• Electrical outlet, Europe / Worldwide
• Petcock (air, gas, vacuum)
- North America (American) style
- Europe / Worldwide style DIN 12898,
DIN 12919, DIN 3537
Cabinet Accessories
• PVC armrest
-
Chemically treated, improves operator
comfort, easy-to-clean. 712 mm
(28") standard size.
• Germicidal UV lamp
- Controlled by automatic UV lamp
timer through Sentinel micro-
processor control panel.
- Emission of 253.7 nanometers for
most efcient decontamination.
- Lamp is positioned away from
operator line-of-sight for safety and
proper exposure to interior surfaces.
Note: UV lamp intensity reduces over
time and its effectiveness is subject to
factors such as relative humidity in the
cabinet, ambient air temperature and
microbial species in the work zone.
• Ergonomic lab chair
- Laboratory grade construction,
meets Class 100 cleanliness; alcohol
resistant PVC materials
-
Adjustable 395-490 mm (15.6"-19.3")
• Ergonomic foot rest
- Angled, helps maintain proper
posture.
- Adjustable height
- Anti-skid coating, chemical
resistant nish.
• IV bar, with hooks
- Stainless steel construction
-
Available for all standard Esco cabinets.
• Microscope viewing device
- Mounting and viewing pouch
integrated into sash. Factory installed;
specify when ordering.
• Solenoid valve
- Interlocked via the microprocessor
control system to shut off gas
supply when the blower is off, sash
at unsafe position or in case of an
airow alarm. Factory installed;
specify when ordering.
Dynamic Chamber™Plenum Design
Negative pressure Positive pressure
The Esco double-wall design creates a Dynamic
Chamber plenum which surrounds contaminated
areas with negative pressure, preventing the possibility
of contamination from leaks in filter seal, gasket or
cabinet structure.
Standards
Compliance
Biosafety Cabinets Air Quality Filtration Electrical Safety
EN 12469, Europe
ISO 14644.1, Class 3, Worldwide
JIS B9920, Class 3, Japan
JIS BS5295, Class 3, Japan
US Fed Std 209E, Class 1 USA
EN-1822 (H14), Europe
IEST-RP-CC001.3, USA
IEST-RP-CC007, USA
IEST-RP-CC034.1, USA
UL-61010-1, USA
CAN/CSA-22.2, No.61010-1
EN-61010-1, Europe
IEC- 61010-1, Worldwide

77
Model LP2 Biological Safety Cabinet Technical Specifications
1. Exhaust filter , H14
2. Blower
3. Downflow filter, H14
4. UV light Retrofit Kit provision
5. IV-Bar Retrofit Kit provision
6. Service fixture Retrofit Kit provision for gas,
air and vacuum
7. Electrical outlet Retrofit Kit provision
(2 single outlet)
8. Electrical & Electronic Panel
9. Fluorescent lamp
10. Stainless steel armrest
11. Esco Sentinel microprocessor control system
12. Motorised Sash Glass Window
13. Multi-piece stainless steel work surface
14. Exhaust collar (Optional)
Optional Exhaust Collar Positions for Thimble-Ducting for LP2 Models
Side View - All Sizes
320 mm
(12.6")
Top View - LP2-6S_
Top View - LP2-4S_
450 mm
(15.9")
320 mm
(12.6")
620 mm
(24.4")
250 mm
(ø9.8")
320 mm
(12.6")
173 mm
(6.8")
8
9
10
570 mm (22.4")
2
9
4
9
5
9
6
9
7
670 mm (26.4")
1390 mm (54.7") 250 mm
9.8"
(40.7"/ 52.8"/ 64.8"/ 76.8")
1035 mm / 1340 mm / 1645 mm / 1950 mm
(35.9"/ 47.9"/ 59.9"/ 71.9")
911 mm / 1216 mm / 1521 mm / 1826 mm
11
12
13
14
3
1

Biological Safety Cabinets • Class II Biological Safety Cabinets
88
General Specifications, Labculture Plus Biological Safety Cabinets (S-Series)
Note to customer: Insert electrical voltage number into last model number digit _ when ordering.
Model LP2-4S_ LP2-6S_
Nominal Size 1.2 meters ( 4') 1.8 meters ( 6')
External Dimension
(W x D x H)
Without Base Stand 1340 x 740 x 1390 mm
52.8" x 29.1" x 54.7"
1950 x 740 x 1390 mm
76.8" x 29.1" x 54.7"
With Optional Base Stand, 711
mm (28") type
1340 x 740 x 2101 mm
52.8" x 29.1" x 82.7"
1950 x 740 x 2101 mm
76.8" x 29.1" x 82.7"
Internal Dimension (W x D x H) 1216 x 570 x 670 mm
47.8" x 22.4" x 26.4"
1826 x 570 x 670 mm
71.9" x 22.4" x 26.4"
Usable Work Area 0.54 m2 (5.8 sq.ft.) 0.83 m2 (9.0 sq.ft.)
Tested Opening 175 mm (7")
Working Opening 200 mm (8")
Average Airflow
Velocity
Inow 0.45 m/s (90 fpm)
Downow 0.3 m/s (60 fpm)
Airflow Volume
Inow 341 m3 / h (201 cfm) 512 m3 / h (302 cfm)
Downow, 68% 722 m3 / h (425 cfm) 1085 m3 / h (640 cfm)
Exhaust, 32% 341 m3 / h (201 cfm) 512 m3 / h (302 cfm)
Required Exhaust With Optional
Thimble Exhaust Collar 512 m3 / h (301 cfm) 768 m3 / h (452 cfm)
ULPA Filter Typical Efficiency >99.995% for particle size between 0.1 to 0.3 microns per IEST-RP-CC001.3
Sound Emission
NSF / ANSI 49 <63 dBA <59 dBA
EN 12469 <60 dBA <56 dBA
Fluorescent Lamp Intensity >1100 Lux (>102 foot candles) >1100 Lux (>102 foot candles)
Cabinet Construction
Electrogalvanized steel with Isocide oven-baked epoxy-polyester powder coating
1.2 mm (0.05") /18 gauge
Electrical*
220-240V, AC, 50Hz, 1 LP2-4S1 LPS-6S1
Cabinet Power/ Amp 700 W / 4 A 920 W / 5.2 A
Outlet Amp Fuse 5 A 5 A
Total Amp 9 A 10.2 A
BTU/ Hr 1428 1877
220-240V, AC, 60Hz, 1 LP2-4S3 LP2-6S3
Cabinet Power/ Amp 700 W / 4 A 920 W / 5.2 A
Outlet Amp Fuse 5 A 5 A
Total Amp 9 A 10.2 A
BTU/ Hr 1428 1877
Net Weight ** 234 kg / 516 lbs 304 kg / 669 lbs
Shipping Weight ** 281 kg / 620 lbs 365 kg / 803 lbs
Shipping Dimensions, Maximum (W x D x H) ** 1400 x 860 x 1750 mm
55.1" x 33.9" x 68.9"
2010 x 860 x 1750 mm
79.1" x 33.9" x 68.9"
Shipping Volume, Maximum ** 2.11 m3 (74.5 cu.ft.) 3.03 m3 (106.9 cu.ft.)
* Additional voltages may be available; contact Esco for ordering information.
** Cabinet only, excludes optional stand.

99
Microbiological Testing
Esco performs testing in accordance
with more than 10 of the world’s most
recognized standards for local, regional
and international criteria. Testing in our
microbiology laboratory is conducted
according to NSF / ANSI 49, EN 12469, and
JIS K3800. An NSF-accredited biohazard
cabinet eld certier is available in-house
full-time to supervise all testing work.
Harmless Bacillus atrophaeus (formerly
Bacillus Subtilis) bacteria is used to
challenge the cabinet, then incubated for
48 hours and the Colony Forming Units
(CFU) are counted to determine the testing
results. Increased microbiological challenge
tests with objects inside the cabinet work
zone, Bunsen burner, external airflow
disturbance, and Human-As-Mannequin
test adapted from Fume Hood development
were performed to simulate real-world
conditions.
Personnel Protection Test
The test objective is to evaluate the safety
of the cabinet for the personnel operating
on potentially hazardous samples in the
cabinet workzone.
• A nebulizer containing 55 mL of 5 to 8
x 108 spores/mL B.atrophaeus spores is
placed inside the workzone, 10 cm (4
inches) behind the front opening sash.
• Target slit air samplers and impingers are
placed outside the workzone to capture
possibly escaping B.atrophaeus spores,
then the sample is incubated
• Acceptance: The number of Bacillus
atrophaeus CFU recovered from the agar
plates shall not exceed 10 CFU per test.
Product Protection Test
The test objective is to determine cabinet
protection to the product/samples inside
the cabinet workzone from environmental
contaminants.
• A nebulizer containing 55 mL of 5 to 8
x 106 spores/mL B.atrophaeus is placed
at 10 cm (4 inches) in front of sash
window.
• Target agar plates are placed throughout
the entire work surface.
• Acceptance: The number of Bacillus
atrophaeus CFU recovered from the
agar plates shall not exceed 5 CFU
per test.
Cross Contamination Test
The test objective is to evaluate cabinet
protection from cross contamination of
samples placed simultaneously inside the
workzone.
• A nebulizer containing 55 mL of spores
(5 to 8 x 104/mL) is placed against one
of the workzone sidewalls.
• Target agar plates are placed 36 cm
(14”) away from the same side wall
• Acceptance: The number of Bacillus
atrophaeus CFU recovered on agar
plates shall not exceed 2 CFU per test.
HPV Test Compliant:
Safer Hydrogen Peroxide
Decontamination Compatibility
Esco biological safety cabinets are
Hydrogen Peroxide Vapor (HPV) compliant
and decontaminatable cabinets approved
by BIOQUELL for this patented process.
HPV is a safer and more efcient alternative
to conventional decontamination using
formaldehyde (CH20):
• HPV is non-carcinogenic and odorless,
while formaldehyde is carcinogenic,
toxic and has pungent smell.
All exterior painted surfaces are powder-coated
with Esco Isocide, an antimicrobial inhibitor to
minimize contamination. Isocide is integrated into
the coating substrate and cannot wash out or
diminish by repeated cleaning. Performance results
are available upon request. Contact Esco or your
Esco Sales Representative for details.
�
04hours 12 hours8 hours 16 hours 20 hours 24 hours
2
4
6
8
Contact Time in Hours
Bacterial Population (10 folds)
Antimicrobial Powder-Coating
Personnel Protection Test Product Protection Test Cross Contamination Test

Biological Safety Cabinets • Class II Biological Safety Cabinets
1010
• If there is a gap on the cabinet sealing,
escaping HPV to the lab will decompose
to become oxygen and water. Escaping
formaldehyde, however, is harmful
to people in the lab. Therefore HPV
decontamination can be performed
while people still working inside the lab,
while formaldehyde decontamination
must be performed with no one present
in the lab. The HPV method improves
safety, productivity, and reduces the
time to seal the cabinet.
• HPV biological efcacy is independent
of environmental variables, whereas
formaldehyde efcacy is dependent on
such variables.
• HPV has a better penetration capacity,
resulting in a full decontamination of
the cabinet. The formaldehyde method
is known to result in incomplete
decontamination.
• HPV is more effective and rapid against
biological organisms compared to
formaldehyde.
• HPV requires approximately 4-7 hours for
set-up, decontamination, and tear-down,
compared to a total of 12-15 hours
needed to complete a formaldehyde
decontamination process.
• HPV decontamination effectiveness
is independent of temperature and
humidity. Formaldehyde requires
temperature above 20°C and relative
humidity above 65%.
• For information on the BIOQUELL HPV
methodology contact Esco or your Sales
Representative.
KI Discus Containment
Test According to EN 12469
(Potassium Iodide)
Esco is currently one of the few compa-
nies in the world equipped to perform
the KI Discus test for our customers. The
KI Discus test is dened in the European
Standard for microbiological safety cabi-
nets, EN 12469, as a test method for vali-
dating the operator/personnel protection
capabilities of the cabinet.
• The KI Discus test shows excellent
correlation with the microbiological
test method for operator protection,
and is useful for validating the actual
containment performance of the
cabinet on-site.
• The KI-Discus takes only 45 minutes as
opposed to 2 days for microbiological
testing.
• Thus, each Esco Labculture Plus (LP2)
model is type tested using the KI Discus
method for operator safety.
Purchase Specifications
LP2 Series Class II, Biological Safety Cabinet
General Performance and Certifications
1. The biological safety cabinet shall comply with
the following international standards, and the
manufacturer shall provide a certied copy of
containment and performance tests equivalent
to or greater than specified in the following
independent international standards for
biological safety, electrical and other functional
characteristics: Class II per EN 12469.
2. The cabinet shall protect (a) the operator and
laboratory environment from particulates
generated within the work zone; (b) the product
and process within the work zone from airborne
contamination from ambient air; (c) and the
product and process within the work zone from
cross contamination.
4. Each cabinet shall be listed by CE for electrical
safety.
5. Original documentation specic to each cabinet
serial number shall be provided with the cabinet
and maintained in the manufacturers’ records.
Test data verifying all performance criteria shall be
available upon request to include: (a) inow velocity
through direct inow measurement method; (b)
downow velocity and uniformity; (c) lter leak
scan with aerosol challenge for both lters; (d)
light, noise, vibration; (e) and electrical safety.
Filtration System
6. The cabinet shall have one supply downow lter
and one exhaust lter. Both lters shall be ULPA-
type per IEST-RP-CC001.3 and meet EN1822
(H14) requirements.
7. The lters shall be within an aluminum frame with
mini-pleat design without aluminum separators;
no wood or berboard shall be used in the lter
assembly.
8. Typical lter efciency shall be 99.999% for 0.1
to 0.3 microns.
9. An integral lter guard shall be afxed to prevent
damage to the lter media.
10. The lters shall be (a) individually scan tested by
the manufacturer, (b) individually scan tested after
assembly, and (b) easily accessible for scan testing
in situ by means of a dedicated upstream sampling
port accessible from within the cabinet.
Every Labculture Plus LP2 model manufactured by Esco
is individually tested, documented by serial number and
validated with the following test methods.
Inflow / downflow velocity
PAO aerosol challenge for filter integrity
Light, noise and vibration
Airflow pattern visualization
Electrical safety to IEC61010-1
KI-Discus containment
Additional microbiological testing is performed
on statistical sampling basis.
Comprehensive Performance Testing At Esco

1111
11. The supply lter shall be angled and oriented to
the 5° cabinet front angle to maximize downow
uniformity over the work surface.
12. A removable, perforated metal diffuser shall
be installed below the supply lter to optimize
airow uniformity and to protect from damage.
Blower System
13. The cabinet shall have a direct drive, permanently
lubricated centrifugal blower/motor dynamically
balanced in two planes compliant to ISO2710 for
low noise, low vibration and long lter life.
14. The blower/motor shall have an external rotor
design and include an automatic thermal cut-
out to disable the motor in case of overheating.
15. The blower/motor shall have an automatic speed
control to compensate for lter loading.
16. The blower/motor system shall be enclosed
within a dynamic chamber shaped steel plenum
and integrated with the removable supply lter
assembly to simplify lter changing.
Cabinet Design, Construction, Cleaning
18. The cabinet shall be of double wall design
whereby all positive pressure plenums capable of
handling contaminated air shall be surrounded by
negative pressure. No positive pressure areas shall
be accessible external to the cabinet.
19. The cabinet shall maintain containment
performance even when removable work area
components are removed for cleaning.
20. Single-piece or multi-piece tray, removable,
stainless steel with radius corners without crevices
or joints.
21. The cabinet shall have a stainless steel, one-
piece fabricated drain trough with open angles
to channel spills to a common drain.
22. The closed sidewall shall be sealed without
perforations, return air slots or concealed areas
which can contain contaminants.
23. The cabinet shall be free of sharp edges, non-
functional protrusions, bolts, screws or hardware,
and all metal edges shall be deburred.
Ergonomics and Convenience
25. The front sash shall be frameless to maximize
visibility, and accessible for cleaning front and
back. Sash glass shall be safety glass.
26. Motorized front sash allows nger-tip operation.
27. Magnetic, not mechanical, proximity sensors shall
work in conjunction with the control system to
indicate proper sash position for containment.
28. Fluorescent lamps shall be mounted behind the
control panel module out of the work zone.
Electronic ballasts shall be used to eliminate icker,
extend lamp life and reduce heat output.
29. The UV lamp shall operate via an automatic
timer with automatic shut-off managed by the
microprocessor controller and shall be interlocked
with the blower/motor and uorescent lights for
safety.
30. The cabinet shall be designed with a 5° angled
front to optimize user comfort, reduce glare and
maximize reach into the work area.
31. Penetrations for petcocks and service fittings
shall be provided; penetrations shall be offset to
improve user access.
32. The cabinet shall accommodate an optional
mounting stand for xed-height or adjustable-
height congurations.
Control and Alarm System
33. All cabinet functions shall be managed by a
programmable microprocessor control system
capable of software updates via Internet
downloads.
34. The microprocessor controller shall be mounted
on the main control panel facing down toward
the user.
35. The controller shall include soft-touch keypad
controls and backlit LCD displays to permit
operation of the blower/motor, light, UV lamp,
electrical outlet(s) and menu.
36. The controller shall be user programmable in
situ to enable or disable functions such as PIN
(personal identication number) access restriction,
cabinet start-up protocol, airow alarm and other
microprocessor controlled operations outlined in
the user manual.
37. When programmed ON, the start-up protocol shall
perform an automatic pre-purge and post-purge
cycle to ensure proper cabinet operation.
38. The controller shall include a blower/motor hours
meter to display aggregate motor running time to
assist in predictive maintenance.
39. Audible and visual alarms shall be provided for
unsafe conditions such as improper airow or sash
position.
40. Airow shall be monitored by two temperature
compensating, thermistor-based, true air velocity
sensor mounted in the cabinet.
41. The airflow display and alarm system shall be
individually calibrated before shipment.
42. The main control panel shall exhibit continuous
display of air velocity and a 24-hour clock display.
Certification, Service and Decontamination
43. The cabinet shall be approved for both hydrogen
peroxide vapor (HPV) and formaldehyde
decontamination protocol.
44. All panels leading to potentially contaminated
and/or hazardous areas shall be color coded red.
45. All components with the exception of blower/
motor and ULPA lters shall be located outside
of contaminated air spaces to facilitate servicing
without the need to decontaminate the cabinet.
46. All exterior surfaces shall be painted with a
permanent antimicrobial inhibitor coating to
minimize contamination.
47. Each cabinet shall undergo the following tests in
the factory.
- Inow/ downow velocity
- Filter leaks test
- Light, noise, and vibration
- Electrical safety to IEC 61010-1
- KI-Discus containment
Esco builds quality from inside out.
Multi-piece tray, removable, stainless
steel with radius corners

12
NSF / ANSI 49 Biological Safety Cabinets • Animal Containment Workstations • Fume Hoods • Clean Benches
Esco Technologies, Inc. • 2940 Turnpike Drive, Units 15-16 • Hatboro, PA 19040, USA
Toll-Free USA and Canada 888-375-ESCO • Tel [phone] • Fax [phone]
us.escoglobal.com • [email protected]
Esco Micro Pte. Ltd. • 21 Changi South Street 1 • Singapore 486 777
Tel [phone] • Fax [phone] • [email protected]
www.escoglobal.com
©2008 Esco Micro Pte. Ltd. Specications Subject to Change. ES1253_V1_3K_02/08
Esco Containment, Clean Air and Laboratory
Equipment Products
Biological Safety Cabinets, Class II, III
Fume Hoods, Conventional, High Performance, Ductless Carbon Filtered
Laminar Flow Cabinets, Horizontal, Vertical, PCR
Animal Containment Workstations
Hospital Pharmacy Isolators, Cytotoxic Safety Cabinets
Specialty Workstations: In-Vitro Fertilization, Powder Weighing
PCR Thermal Cyclers, Conventional, Real-Time
Cleanroom Fan Filter Units, Modular Rooms, Air Showers, Pass Thrus
Since 1978, Esco has emerged as a leader in the development of controlled environment, laboratory and cleanroom
equipment solutions. Products sold in more than 100 countries include biological safety cabinets, fume hoods, ductless
fume hoods, laminar flow clean benches, animal containment workstations, cytotoxic cabinets, hospital pharmacy isolators,
and PCR cabinets and instrumentation. With the most extensive product line in the industry, Esco has passed more tests,
in more languages, for more certifications, throughout more countries than any biosafety cabinet manufacturer in the
world. Esco remains dedicated to delivering innovative solutions for the clinical, life science, research and industrial
laboratory community. www.escoglobal.com.
LIVE SUPPORT
escoglobal.com
PT Esco Bintan Indonesia
Cert. No: 651333/1
Esco Micro Pte Ltd
Cert. No: 651333
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