avantor VWR Mega Star 600 Series User manual

Instruction manual
VWR®Mega Star 600/600R Centrifuge Series
Cat. no 76533-880 Mega Star 600 without rotor
76533-884 Mega Star 600 package
76533-882 Mega Star 600R without rotor
76533-886 Mega Star 600R package
___
Version a Issue 06/2022

2 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Legal Address of Manufacturer
North America
Avantor
One Radnor Corporate Center
Building One, Suite 200
100 Matsonford Road
Radnor, PA 19087
1 610 386 1700
Country of origin
VWR Mega Star 600R:
VWR Mega Star 600:
Germany
China

3 v w r. c o m I Instruction manual VWR®Mega Star 600/600R

4 v w r. c o m I Instruction manual VWR®Mega Star 600/600R

5 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Table of Contents
Legal Address of Manufacturer.................................................................................... 2
Warning.......................................................................................................................... 7
Safety Information ..................................................................................................................................... 7
Set Up Conditions ..................................................................................................................................... 7
Preparation................................................................................................................................................ 8
Hazardous Substances ............................................................................................................................. 8
Operating................................................................................................................................................... 9
Maintenance.............................................................................................................................................. 9
Package Contents ....................................................................................................... 10
Delivery Content...................................................................................................................................... 10
Unpacking and Installation......................................................................................... 10
Before setting up ..................................................................................................................................... 10
Location ................................................................................................................................................... 10
Transporting ............................................................................................................................................ 11
Levelling .................................................................................................................................................. 11
Mains Connection.................................................................................................................................... 12
Storage .................................................................................................................................................... 12
Shipping................................................................................................................................................... 12
Intended use ................................................................................................................ 13
Symbols and conventions.......................................................................................... 13
Symbols used on the centrifuge.............................................................................................................. 13
Product Specifications ............................................................................................... 14
Technical data ......................................................................................................................................... 14
Refrigerant............................................................................................................................................... 15
Mains supply............................................................................................................................................ 16
Directives, Standards and Guidelines ..................................................................................................... 16
Description of Buttons and Switches........................................................................ 17
Control panel ........................................................................................................................................... 17
VWR Mega Star 600 ............................................................................................................................... 17
VWR Mega Star 600R............................................................................................................................. 18
Getting Started and Operation ................................................................................... 19
Switch on centrifuge ................................................................................................................................ 19
Open the Centrifuge Door ....................................................................................................................... 19
Close the Centrifuge door ....................................................................................................................... 19
Acceleration / Deceleration Rates........................................................................................................... 21
Programs ................................................................................................................................................. 24
Centrifugation .......................................................................................................................................... 24
Short-term centrifugation......................................................................................................................... 26
Removing the Rotor ................................................................................................................................ 26
Aerosol-tight rotors .................................................................................................................................. 27
Switch off Centrifuge ............................................................................................................................... 27

6 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
System Menu ............................................................................................................... 27
Flowchart System Menu.......................................................................................................................... 27
Troubleshooting .......................................................................................................... 29
Mechanical emergency door release ...................................................................................................... 29
Troubleshooting by Guide ....................................................................................................................... 31
When to contact customer service .......................................................................................................... 32
Repair and maintenance............................................................................................. 33
Maintenance and care............................................................................................................................. 33
User replaceable accessories and spare parts ........................................................ 37
Technical service ........................................................................................................ 37
Warranty....................................................................................................................... 38
Compliance with local laws and regulations ............................................................ 38
WEEE Conformity........................................................................................................ 39
APPENDIX.................................................................................................................... 40

7 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Warning
Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration
of the seller’s warranty.
Safety Information
WARNING
Observe the safety instructions. Not following these instructions can cause damage.
The centrifuge is to be used for its intended use only. Improper use can cause damages,
contamination and injuries with fatal consequences.
The centrifuge should be operated by trained specialists only.
In order to ensure safe operation of the VWR Mega Star 600 / 600R Centrifuge, general safety regulations
must be followed. Mind the regulations in your country.
Set Up Conditions
WARNING
•Plug the centrifuge only into sockets which have been properly grounded.
•Turn off the centrifuge at the main switch. The mains plug must be freely accessible at
all times.
Press the STOP key to shut down the centrifuge.
Pull out the power supply plug or disconnect the power supply in an emergency.
•As a safety zone, maintain a clear radius of at least 30 cm around the centrifuge.
Do not place any dangerous substances within this security zone.
•Set up in a well-ventilated environment, on a horizontally levelled and rigid surface
with adequate load-bearing capacity.

8 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Preparation
WARNING
•It is the obligation of the operator to make sure, that the proper protective clothing is
used. Mind the “Laboratory Biosafety Manual” of the World Health Organization
(WHO) and the regulations in your country.
•Do not make any changes to the mechanical components of the rotor.
•Do not touch the electronic components of the centrifuge nor alter any electronic or
mechanical components.
•Use only with rotors which have been properly installed. Follow the instructions on the
Auto-Lock™rotor exchange.
•Do not use rotors, buckets or accessories which show any signs of removed protective
coating, corrosion or cracks. Contact customer service for further advice or
inspections.
•Use only with rotors which have been loaded properly.
•Never overload the rotor.
•Always balance the samples.
•Use only rotors and accessories for this centrifuge which have been approved by
VWR.
Exceptions to this rule are commercially available glass or plastic centrifuge labware,
provided they have been designed to fit rotor or adapter cavities and are approved for
the speed or the RCF value of the rotor.
•Make sure the rotor is locked properly into place before operating the centrifuge.
•Implement measures which ensure that no one can approach the centrifuge for longer
than absolutely necessary while it is running.
•If used for foodstuffs, machinery for cosmetics or pharmaceutical products, only use
closed or aerosol-tight containers for centrifugation.
Hazardous Substances
WARNING
•Especially when working with corrosive samples (salt solutions, acids, bases), the
accessory parts and vessel have to be cleaned thoroughly.
•Do not centrifuge explosive or flammable materials or substances.
•The centrifuge is neither inert nor protected against explosion. Never use the
centrifuge in an explosion-prone environment.
•Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms
without suitable safety precautions.

9 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of the World Health
Organization (WHO) and any local regulations. When centrifuging microbiological samples from the Risk
Group II (according to the “Laboratory Biosafety Manual” of the World Health Organization (WHO)),
aerosol-tight biological seals have to be used. Look on the internet page of the World Health Organization
(www.who.int) for the “Laboratory Biosafety Manual”.
For materials in a higher risk group, extra safety measures have to be taken.
•If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate
disinfection measures have to be taken.
•Extreme care should be taken with highly corrosive substances which can cause damage and
impair the mechanical stability of the rotor. These should only be centrifuged in fully sealed tubes.
•If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area
immediately.
Operating
WARNING
•Never use the centrifuge if parts of its cover panels are damaged or missing.
•Never start the centrifuge when the centrifuge door is open.
•Do not move the centrifuge while it is running.
•Do not lean on the centrifuge.
•Do not place anything on top of the centrifuge during a run.
•Never open the centrifuge door until the rotor has come to a complete stop and this
has been confirmed in the display.
•The emergency door release may be used in emergencies only to recover the
samples from the centrifuge, e.g. during a power failure.
Do not open the centrifuge, while it is running.
In any case of severe mechanical failure, such as rotor or bucket crash, the centrifuge is not aerosol-tight.
In case of rotor failure, the centrifuge can be damaged. Leave the room. Inform customer service.
Maintenance
WARNING
The centrifuge housing is not to be opened by the operator.

10 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Package Contents
Description Quantity
VWR Mega Star 600 / 600R Centrifuge 1
Power supply cable 2
Instruction manual 1
If any parts are missing, please contact the nearest VWR representative.
Delivery Content
Part No. Package
76533-884 VWR MEGA STAR 600 PKG
76533-886 VWR MEGA STAR 600R PKG
Unpacking and Installation
Before setting up
1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and
VWR immediately if any damage is discovered.
2. Remove the packaging.
3. Check, if the items supplied are complete.
If the order is incomplete, please contact VWR.
Location
The centrifuge should only be operated indoors.
The set-up location must fulfillthe following requirements:
•Asafety zone of at least 30 cm must be maintained around the centrifuge.
People and hazardous substances must be kept out of this safety zone while centrifuging.
•The supporting structure must be stable and free of resonance.
•The supporting structure must be suitable for horizontal setup of the centrifuge.
•The centrifuge is not to be exposed to heat and strong sunlight.
WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
•The set-up location must be well-ventilated at all times.

11 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Transporting
WARNING Always lift the centrifuge from both sides. Never lift the centrifuge by its front panel,
its back panel or at its door.
Always remove the rotor before moving the centrifuge.
Due to its weight, the centrifuge should be carried by at least two people. Always lift the centrifuge at both
sides.
To prevent possible injuries, at least two people should lift and carry the centrifuge by holding it at the
bottom from opposite sides.
Transport the centrifuge and accessories upright within the associated packaging, if possible.
NOTICE
Store the original centrifuge packaging. Contact a shipping company or the customer service
for the transport.
Always remove the rotor before moving the centrifuge. If you do not remove the rotor you
might damage the centrifuge drive or centrifuge spindle.
Levelling
CAUTION If the centrifuge isn’t levelled, imbalances can occur, and the centrifuge can be
damaged.
Do not place anything under the centrifuge feet to level the centrifuge.
The centrifuge is to be placed on horizontal and level supporting structures or benching.
Horizontal level is to be checked when moving the centrifuge to a new location.

12 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Mains Connection
NOTICE
Plug the centrifuge into grounded electrical sockets only.
Power supply switch on the backside of the
VWR Mega Star 600
Power supply switch on the right side of the
VWR Mega Star 600R
1. Turn off the power supply switch.
2. Check whether the cable complies with the safety standards of your country.
3. Make sure that the voltage and frequency correspond to the figures on the rating plate.
4. Establish the connection to the power supply with the connecting cable.
Storage
WARNING When removing the centrifuge and accessories from use if necessary clean,
disinfect or decontaminate the entire system. In doubt contact the VWR customer service.
•Before storing the centrifuge and the accessories, it must be cleaned and if necessary disinfected
and decontaminated.
Centrifuge, rotors, buckets and accessories have to be thoroughly dried before storage.
•Store the centrifuge in a clean, dust-free location.
•Be sure to place the centrifuge on its feet.
•Avoid direct sunlight.
Shipping
WARNING Before shipping the centrifuge and accessories you have to clean and if necessary,
disinfect or decontaminate the entire system. In doubt contact the VWR customer service.
Before shipping the centrifuge please bear the following in mind:
•The centrifuge must be clean and decontaminated.
•The decontamination must be confirmed in a decontamination certificate. Contact customer service
for more details.

13 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Intended use
This centrifuge is a laboratory product used to separate substance mixtures of different densities.
This centrifuge has to be operated by trained personnel only.
Symbols and conventions
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to electrical hazards.
Symbols used on the centrifuge
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and
your environment safe.
This symbol refers to information on hazards, described within the manual.
This symbol demands to disconnect mains before transporting or servicing the
centrifuge.
This symbol demands to check, if the rotor is installed correct by lifting it slightly at
the handle.

14 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Product Specifications
Technical data
VWR Mega Star 600
Environmental Conditions -Use in interior spaces
-Altitudes of up to 2000 m above sea level
-Max. relative humidity 80 % up to 31 °C;
decreasing linearly to 50 % relative humidity at 40 °C
Environmental Conditions during Storage
and Shipping
Temperature: -10 °C to +50 °C
Humidity: 15 % to 85 %
Permissible Ambient Temperature during
Operation
+2 °C to +35 °C
Heat Dissipation1120 V, 60 Hz 0.31 kWh/h; 1060 Btu/h; 1120 kJ/h
Overvoltage Category II
Pollution Degree 2
IP 20
Running Time 99 h 59 min 50 sec, hold
Maximum Speed nmax 16000 rpm
Minimum Speed nmin 300 rpm
Maximum RCF Value at nmax 24328 x g
Noise Level at Maximum Speed1< 58 dB (A) (measured with a TX-150 rotor)
Maximum Kinetic Energy 8.12 kJ
Dimensions
Height (open door / closed door)
Width
Depth
660 mm / 310 mm
370 mm
490 mm
Weight235 kg
1Front Side Measurement, 1 m in front of the instrument at 1.6 m height
2Without Rotor

15 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
VWR Mega Star 600R
Environmental Conditions -Use in interior spaces
-Altitudes of up to 3000 m above sea level
-Max. relative humidity 80 % up to 31 °C;
decreasing linearly to 50 % relative humidity at 40 °C
Environmental Conditions during Storage
and Shipping
Temperature: -10 °C to +50 °C
Humidity: 15 % to 85 %
Permissible Ambient Temperature during
Operation
+2 °C to +35 °C
Heat Dissipation1120 V, 60 Hz 0.35 kWh/h; 1170 Btu/h; 1140 kJ/h
Overvoltage Category II
Pollution Degree 2
IP 20
Running Time 99 h 59 min 50 sec, hold
Maximum Speed nmax 17850 rpm
Minimum Speed nmin 300 rpm
Maximum RCF Value at nmax 30279 x g
Noise Level at Maximum Speed1< 52 dB (A) (measured with a MicroClick 24 x 2 rotor),
< 56 dB (A) (measured with a TX-150 rotor)
Maximum Kinetic Energy 10.1 kJ
Temperature Setting Range -10 °C to +40 °C
Dimensions
Height (open door / closed door)
Width
Depth
700 mm / 320 mm
460 mm
670 mm
Weight274 kg
1Front Side Measurement, 1 m in front of the instrument at 1.6 m height
2Without Rotor
Refrigerant
Part No. Centrifuge Refrigerant Quantity Pressure GWP CO2e
76533-882 VWR Mega Star 600R R-134a 0.265 kg 21 bar 1430 0.38 t
Contains fluorinated greenhouse gases in a hermetically sealed system.

16 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Mains supply
The following table contains an overview of the electrical connection data. This data is to be taken into
consideration, when selecting the mains connection socket.
Unit VWR Mega Star 600 VWR Mega Star 600R
Mains Voltage 120 V ±10 % 120 V ±10 %
Frequency 60 Hz 60 Hz
Rated Current 5 A 8 A
Power Consumption 310 W 700 W
Equipment Fuse 10 AT 15 AT
Building Fuse 15 AT 15 AT
Directives, Standards and Guidelines
Region Directive Standard
North America
120V 60 Hz
ANSI/UL 61010-1
UL 61010-2-020

17 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Description of Buttons and Switches
Control panel
The control panel contains the keys and displays of the centrifuge (only the power switch is located on the
right side (VWR Mega Star 600R) or on the backside (VWR Mega Star 600) of the device).
VWR Mega Star 600
No Function Display Contents
1 Status The status of the centrifuge is displayed here.
2 Speed / RCF Value The speed (rpm) or RCF value (x g) is displayed here.
3 Running Time The running time is displayed here.
4 Acceleration / Deceleration
Profiles
Press the key multiple times to cycle through the available
profiles.
5 PULSE Key Press the PULSE key to immediately start the centrifugation run
and accelerate up to the maximal permissible end speed
(depending on the used rotor). Releasing the key initiates,
a stopping process at the highest braking curves.
6 OPEN Key Press the OPEN key to activate the automatic door release
(possible only when device is switched on and when the rotor is
fully stopped).
7 STOP Key Press the STOP key to manually end the centrifugation run.
8 START Key Press the START key to start a centrifugation run or to accept the
current settings.
9 Arrow Keys Use these keys in order to modify the displayed value.
10 TOGGLE Key for Speed / RCF
Value
Use the TOGGLE key to change the display mode.
(Speed / RCF Value).
11 Program Keys Use the Program Keys to save and load programs.

18 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
VWR Mega Star 600R
No Function Display Contents
1 Status The status of the centrifuge is displayed here.
2 Speed / RCF Value The speed (rpm) or RCF value (x g) is displayed here.
3 Running Time The running time is displayed here.
4 Temperature The temperature is displayed here.
5 Acceleration / Deceleration
Profiles
Press the key multiple times to cycle through the available
profiles.
6 PULSE Key Press the PULSE key to immediately start the centrifugation run
and accelerate up to the maximal permissible end speed
(depending on the used rotor). Releasing the key initiates,
a stopping process at the highest braking curves.
7 OPEN Key Press the OPEN key to activate the automatic door release
(possible only when device is switched on and when the rotor is
fully stopped).
8 STOP Key Press the STOP key to manually end the centrifugation run.
9 START Key Press the START key to start a centrifugation run or to accept the
current settings.
10 SNOWFLAKE Key Use the key for prewarming or precooling the centrifuge.
11 Arrow Keys Use these keys in order to modify the displayed value.
12 TOGGLE Key for Speed / RCF
Value
Use the TOGGLE key to change the display mode.
(Speed / RCF Value).
13 Program Keys Use the Program Keys to save and load programs.

19 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Getting Started and Operation
NOTE Only display pictures of the VWR Mega Star 600R centrifuge are shown. The display of the VWR
Mega Star 600 is the same, only the missing details for temperature.
Switch on centrifuge
1. Turn on the power switch of the device.
The device performs a self-check of its software.
a. When the centrifuge door is closed the display shows:
The speed and time display read “0” and “00:00”; the current temperature inside the rotor chamber
is displayed.
b. When the centrifuge door is open the display shows:
The speed and time display show the pre-set values; the set temperature inside the rotor
chamber is displayed.
Open the Centrifuge Door
Press the OPEN key.
Close the Centrifuge door
WARNING Do not reach into the gap between the centrifuge door and the housing.
Use the emergency release only for malfunctions and power failures
Ensure that the centrifuge platform is clear from objects.
Keep hands and objects well clear of the underside and side of the centrifuge door when closing.
Close the centrifuge door by pressing down on it lightly in the middle or on both sides of it. The centrifuge
door mechanism will click and lock in place. Lids should not be slammed as excessive force may cause
damage or disrupt samples.
NOTICE The centrifuge door should audibly click into place.

20 v w r. c o m I Instruction manual VWR®Mega Star 600/600R
Rotor Installation
CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the
centrifuge.
The centrifuge is equipped with an Auto-Lock-locking system.
This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be
bolted on to the centrifuge spindle.
Proceed as follows:
1. Open the centrifuge door and if necessary, remove any dust, foreign objects or residue from the
chamber.
Auto-Lock and O-Ring must be clean and undamaged.
2. Place the rotor over the centrifuge spindle and lower vertically and slowly down the centrifuge
spindle. If necessary, a light push may be required to ensure connection.
The rotor clicks automatically into place.
CAUTION Do not force the rotor onto the centrifuge spindle.
If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle with
little pressure.
3. Check if the rotor is properly installed by lifting it slightly on the handle or from beneath the rotor.
If the rotor can be pulled up, then it must be reconnected to the centrifuge spindle.
WARNING If the rotor cannot be properly locked in place after several attempts, then the
Auto-Lock is defective, and you are not permitted to operate the rotor.
Check for any damage to the rotor and the spindle: Damaged rotors must not be used.
Keep the centrifuge spindle area of the rotor clear of objects.
If in doubt, please call VWR customer service.
CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by
pulling it at its handle or from beneath the rotor. The rotor has to be locked tight.
Auto-Lock
O-Ring
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