N-BIOTEK NB203QR User manual

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NB203QR Operation Manual
CO2 incubator + Roller
Model : NB-203QR

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Table of Contents
NO.
CONTENTS
PAGE
1
SAFETY AND GENERAL PRECAUTION
3
2
TRANSPORTATION, STORAGE,
AND LOCATION OF INSTALLATION
5
3
PREREQUISITE AND CONFIGURATION
7
4
FEATURES AND SPECIFICATIONS OF THE UNIT
9
5
CONTROL PANEL
10
6
OPERATION
11
7
SAFETY SWITCH
16
8
ROLLER OPERATION
16
9
HOW TO DETACH THE ROLLER
17
10
TROUBLESHOOTING
18

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1. SAFETY AND GENERAL PRECAUTION
1.1 General information on precaution
⚫Precaution is to prevent the possible accident or danger during operation.
So, you must keep it.
⚫Precaution is divided into caution and warning. And, each of them has following meanings.
If you don't keep this warning, you can get an accident or a fire.
If you don't keep this caution, you can get injured as well as a property loss.
1.1.1 Safety warning symbols
1.2 Precaution for using the power cable
Do not make the power plug be pressed by back of the product.
(A space between the product and the plug must be 30cm at least.)
The power outlet must be only for this product.
(Using various products simultaneously can cause a fire.)
Clean the power plug with a dry towel and connect it properly.
(Foreign substances or unsafe connection can cause a fire.)
Do not bend the power cable hardly and do not make it to be pressed by
heavy products. (When it is damaged, it can cause a fire.)
Do not touch the power cord with wet hands. (It can cause an electric shock.)
Do not use the damaged power cord and outlet.
(It can cause an electric shock and a fire.)
When you see smoke coming from the product or smell burning, you have to pull out the power
cord and stop using it. (It can cause an electric shock and a fire.)

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1.3 Precaution for ground connection
Please ground before use the product, if you don't ground, you could get an electric shock
when malfunction or an electric leakage occurs.
At the place where you can't ground,
* Please buy the equipment to prevent any electrical leakage.
* An electric shock, an electric leakage and a fire can be occurred without
an electric leakage breaker.
Do not ground to these places; Gas Pipe, water pipe, pipe, lighting rod,
telephone wire, etc.
* Wrong ground connection can cause electrical leakage which eventually results in fire.
If you don't have the outlet for AC 220V, then bury it under the ground
after connecting the ground line to copper plate.
* No ground connection can bring an electrocution, an electric leakage and a fire.
1.4 Precaution for use
You must not disassemble, fix and remodel the product by yourself.
(You can damage the product throughout a fire and malfunction or get a property
loss as well as experimental loss.
Do not use the product for different purpose.
(It can cause malfunction or poor function. Consequently, you will get a wrong result.)
Do not use an inflammable spray near the product.
(The switch and other electric connection parts can cause a fire.)
When you use inflammable substances such as benzene, thinner, alcohol
and LP gas, please be careful. (It can cause a fire and an explosion.)
To prevent water and experiment material from going into the control
panel during the experiment, make sure to clean the control panel with a
dry cloth. (It can cause an electric leakage and a fire.)
Do not wash the product with excessive quantity of water, thinner, benzene
and Petroleum.
(It can cause an electric leakage, and malfunction or damage on the surface.)

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When you don't use the product or clean it, please pull out the power plug.
(It is to prevent an eclectic leakage.)
Open and close the door softly and please use a door knob.
(A heavy shock can damage the product and breakdown the operating part.
Also your hands can be stuck between the door and body.)
Do not detach the built-in lamp and electrical devices.
(It can cause an electric shock and a fire.)
Please be sure to prevent foreign substances from getting into the sealing silicon
of the door. (The inflow of open air can cause the change of temperature in chamber and
discoloration of the packing part by a foreign substance.)
2. TRANSPORTATION, STORAGE, AND LOCATION OF INSTALLATION
2.1 Transportation
DO NOT try to slide or tilt the unit
Lift the unit on each corner of bottom with the aid of 2 people.
Permissible ambient temperature range for transport: -10℃to 60℃.
2.2 Storage
Do not keep it at place in High Humidity. Permissible ambient humidity: max. 70%
storage in a cold location is the place you transfer the unit to the installation site for start-up,
condensation may form. In this case, Wait at least one hour until the CO2 incubator has
attained temperature and is completely dry.
Please check the voltage & Hertz written on serial label.
(Over-voltage, under-voltage can damage the product and poor performance.)
Do not install at a humid place.
(It causes an electric leakage accident and a corrosive of the product.)
Keep this product out of the direct ray of sun and do not install at a hot place or
a place that is near an electric heat.
(The proper indoor temperature is 20℃~ 30℃.)

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2.3 Location of installation and ambient conditions
Do not put flammable substances near the product.
(It may cause a fire.)
When you install the product, you are required to keep the distance at least 30cm
from the wall. To completely separate the unit from the power supply, power plug
must be disconnected. Install the unit in the way that the power plug is easily accessible
and can be easily pulled in case of danger.
Install the unit at a flat surface, free from vibration and in a well-ventilated location.
(If the ground is not flat, it may cause an excessive vibration of the product.)
When you move the product, do not lay down to its side or reverse the head to bottom.
(It may cause a malfunction.)
When you move the product, hold the door and other movable parts of
the product with a tape. (When the product is moved, the movable door can cause
injury of you and damage of the product.)
When you move the product, you must hold up the product.
(Pushing or pulling the product can damage the bottom part of the product.)

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3. PREREQUISITE AND CONFIGURATION
3.1 Prerequisite
Inspection of Boxes
When you have received the instrument which is packed on pallet, inspect the box carefully for any
damages that may have caused any damages to product during shipping.
Please report any damage to the carrier or to your local N-BIOTEK distributor immediately.
LOCATION
The incubator is designed to operate at temperature 5℃above ambient, and recommended to operate
at minimum ambient (temperature in the place for use), 15℃. Maximum Room Temperature is 32℃.
To avoid place for use this incubator is as below.
1. Near Heater or Freezer(if it may generate heat and affect temperature control of incubator)
2. Near Equipment generating heat or cold air to incubator.
3. Directly Sunlight Exposed to incubator
4. Uneven ground or table head
5. The place where is being vibrated
Cleaning before use
Before conducting cell culture, It is recommended to clean up entire chamber and shelves, water tray by
using at least 70% Ethanol mixed of 30% distilled water and soft clothes.
Inserting shelves
Shelves are mounted onto the shelf racks in such a way that the edge of shelves which is slightly bent up
about 8mm goes to the back of the chamber until it is too close to the back wall. Basically, 3 shelve are
provided. Insert the shelves from top to bottom.
Level the incubator by adjusting feet. Place a small level on the second shelf of the incubator adjust the
levelling fee until the incubator is level and stable.
After inserting the shelves, place humidity tray in the bottom of chamber if humidification is required for your
application.

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3.2 Configuration
Exterior Configuration of CO2 incubator.
<Figure #1>
Control panel
Air jacket
-6 side direct heating
Ventilation fan Power switch
Glass door heater
-Prevent condensation Magnetic door seal
Stainless steel shelve Glass door
- Perforated to improve uniformity
Inner door seal Door latch
- Airtight to prevent CO2 leakage
Outer door
Roller apparatus
Roller rack fixing pin
Water pan
-Heated by 6 sides direct heating for humidity
Outer door heater
-prevent condensation
CO2 inlet
Safety switch
Power cord
Fuse holder
NOTE
1. The figure 1 is to show you the name of each part of exterior incubator.
2. The diameter of blue PVC tube that we use for inflow of gas is 6mm. (total diameter 6mm, hole 4mm)
Connecting Power Cord:
1. Verify your supply voltage matches the voltage of your incubator.
2. Insert the power cord into its receptacle.
3. Plug the cord into power supply outlet.

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4. FEATURES AND SPECIFICATIONS OF THE UNIT
4.1 Features
1. Excellent Uniformity of Temperature, CO2.
2. 6 Side Direct Heat for Temperature Uniformity and Fast Recovery
3. IR CO₂Sensor detects precise density of CO₂
4. Outer Heated Door ensures no condensation on glass door.
5. Microprocessor PID control for Temperature, CO₂
6. Natural Humidification System by water tray and circulation fan.
7. Simple operation for Roller apparatus.
4.2 Specifications
Items
NB-203QR
Temperature
Range
Ambient +5℃to 60℃
Accuracy
±0.25℃at 37℃
Controller
Microprocessor Digital PID
Humidity
70% ~ 80% at 37℃
CO2
Range
0% to 20%
Accuracy
±0.1% at 5% at 37℃
CO2 Increment
0.1%
CO2 Sensor
IR CO2 Sensor
Roller
2 stairs Roller apparatus
RPM
2 rpm to 30 rpm
Accuracy
±0.1 rpm
Increment
0.1 rpm
Time
Range
Continuous or up to 99h 59min
Outer door
Silicon Packing Magnet Door
Inner door
Tempered Safety Glass Door
Control panel display
LED Display
Jacket type
Dry wall type (6 sides direct heating type)
Chamber material
Stainless Steel (304)
Weight
89kg
Capacity / Shelve
179Liter / 1 shelf
Chamber dimension
473x528x710(H)mm
Overall dimension
560x640x940(H)mm
Power
110/220V, 50/60Hz

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5. CONTROL PANEL
1 2 3 4 5 6 7 8 9
18 17 16 15 14 13 12 11 10
1. Alarm ON/OFF switch
2. Safety Activation Indication Lamp: If Safety is activated, it will be ON.
3. Heating Signal : To show status of heating activation at 3 parts.
Glass door means the heater around glass door.
4. Temperature display LED window
5. CO₂Gas % display window
6. Pilot Lamp for CO2 valve: Pilot lamp ON position means inflow of gas
7. Roller RPM display
8. Roller operating pilot lamp (ON running, OFF not running)
9. Roller power lamp
10. Start/Stop button
11. Adjustment button for roller RPM/TIME
12. SET button for roller RPM/TIME
13. Mode key : RPM/TIME selection button
14. Calibration setting button
15. CO₂Gas setting button
16. Adjustment button for Temperature and CO₂
17. Temperature setting button
18. Alarm mute button

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6. OPERATION
* Before Switch ON, insert water tray filling with distilled water. Make sure connection of gas supply. And
Open CO2 gas cylinder or supply with the pressure of regulator set to 4.5Psi or 0.3 bar.
* Please check connection of gas supply. And Open CO2 gas cylinder for supply with the pressure of
regulator set to a certain level.
Co2 incubator set-up process
6-1. Place and install the product.
Install the product at the desired place and check the level in all directions (side by side, front to back
and ground).
6-2. Connect the power
Prior to connect the power plug, make sure that the POWER S/W is off
6-3. Connect the CO2 gas
▶Check whether CO2 gas is leaking at any point of regulator.
If leak is found, please take a measure to stop leaking before supply of CO2 gas to incubator.
▶Clear the air passage for gas input gasket at the rear of the unit.
Also check the gas tube and get rid of any obstacles for smooth gas flow.
▶Before supply of CO2 gas to incubator by gas tube, check the remaining gas volume in CO2 Gas
cylinder.

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▶When previous stage is cleared, connect the gas tube to regulator and incubator.
At this point, Make sure that valves of all the part besides gas cylinder, Regulator are locked.
(④and ⑤have the opposite lock direction each other. ④is clockwise and ⑤is counterclockwise)
▶Open #5(Master valve of cylinder) and #4(the regulator valve), #3 Flow meter. While Flow meter
fully open, do adjust regulator valve at 0.3bar.
Note
Once power is on with all connection for CO2 gas done, please check the solenoid valve works well by
solenoid sounding. Also, observe the CO2 density on display goes up well. Factory setup for initial CO2 gas
supply is up to 3%. This will be covered more at page 16.
6-4. Power switch on
Once power is on, digital readout of current temperature and CO2 % in chamber will be displayed after
below message passed.
6-5. Setting temperature
a. Connect the power, then the LED screen will display the temperature in the chamber.
b. Press the "TEMP/SET" key, then, the LED screen will flicker and display.
c. Then, input the desired temperature by pushing UP (▲) and DOWN (▼).
d. Press "TEMP/SET" key again after putting the desired value. “SAVE” is shown up on the LED
screen as below.
Note
If you don't press "SET" key lastly after set-up, the new set-up value will not be saved at all.
Set-up Temperature range is Ambient -8℃(lowest 15’C at 24’C RT ~ 45℃)
6-6. Setting Up CO2
a. Press "CO2/SET" key. Then, LED screen will flicker continually.
b. Input the desired value of Co2 density by pushing UP (▲) and DOWN (▼) key
c. Press "SET" key again after input. “SAVE” is shown up on LED screen as below.
Note
If you don't press "CO2/SET" key again after set-up, the new set-up value will not be saved at all.

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6-7 Calibration for temperature and CO2
Please follow up below procedure for calibration in case of discrepancy between actual
value (measured by reliable measurement device) in chamber and displayed value.
Measure CO2 density and Temperature after incubator is stabilized in which takes about more
than 2 hours (you might want to perform this stabilization process at night before home)
Please note that low deviation range such as ±0.1 ~ 0.3% may not be corrected precisely by this
calibration.
No.
DISPLAY
FUNCTION
1
Chamber Temperature
2
Door Heater Temperature
3
Glass Door Heater Temperature
4
CO2 Calibration
5
Heating control
6
CO2 gas supply control
7
To apply a new value
a. Press and hold “CAL/SET”for 10 seconds. Then, LED will be flickering as below.
Channel 1 is at chamber’s Main Temp calibration stage.
Press UP (▲) as much as difference from measured value by precise analyzer if it is higher.
Press DOWN (▼) as much as difference from measured value by precise analyzer if it is lower.
Ex) If measured temperature is 38℃and Display shows 37℃, then press up 1℃.
Note
* Calibration range for temperature is ±5℃
* To go to next channel is to press “CAL/SET”button. After 5th channel, the LED is back to
temperature display.

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b. Second Click “CAL/SET” ☞ Outer door’s Temp calibration
Channel 2 is purposed to remove water condensing on glass door caused by high temperature
difference between chamber and outside. Recommend to use calibration at Channel 2 in case of
water condensing on glass door.
Note
Except water condensing on glass door, calibration of channel 2 and 3 is not recommendable.
Check if the water condensing is removed in 3 Hours after calibration of CH2 is done.
c. Third Click “CAL/SET” ☞ Door Frame Heater calibration
Channel 3 is purposed to remove excessive water condensing on glass door caused by high
temperature difference between chamber and outside. When failed to remove water condensing by
calibrating CH2, try to calibrate
d. Fourth Click “CAL/SET” ☞ CO2 density calibration
Channel 4 is at CO2 density calibration stage.
Press UP (▲) as much as difference from measured value by precise analyzer if it is higher.
Press DOWN (▼) as much as difference from measured value by precise analyzer if it is lower.
Ex) If measured CO2 value is 5% and Display shows 4%, then press up 1%.
e. Fifth Click "CAL/SET" ☞ Heating control
* This is pre-programmed mode before releasing from manufacturer.
Push UP (▲) and DOWN (▼) to set the value.

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NOTE
Channel 5 is to set heating control point.
If it is set at 3, then the heating control works from 34’C against setting value i.e. 37’C.
This is in order to minimize overshooting and faster reaching time to setting value.
Therefore, user is kindly required not to change this value.
f. Sixth Click "CAL/SET" ☞ CO2 gas supply control.
* This is pre-programmed mode before releasing from manufacturer.
Push UP (▲) and DOWN (▼) to set the value
NOTE
Channel 6 is to set starting point of solenoid control for CO2 supply.
Factory pre-programmed set point is at 2 (2 for NB203XL, 3 for NB203) to optimize set-up at 5% and it
means that CO₂control valve is in open position until the CO₂density reaches to 3%. From 3% of CO2 to
setting value, solenoid valve takes control of CO₂supply until it gets to setting value.
When above stage is cleared, please press the button to save the new value.
6-8 Alarm
In order to activate alarm system, it should maintain ±1℃, ± 1% to set point for 3 minutes.
This course is recognised as stabilizing process.
After stabilizing process, alarm activation is as below.
Alarm Activation
Temperature (beep beep beep ---)
If it stays out of ±1℃ from set point for 8~9 minutes, it will give you an alarm.
CO2 : (beep -------)
If it stays out of ± 1% from set point for 8~9 minutes, it will give you an alarm.
Door Open :
Alarm is on 1 minute after door opening. No alarm if closed within 1 minute.
After 1 minute of door open with alarming,
-- Alarm will stop 2.5 seconds after door closed.
-- Mute button will give no alarm. But alarm will activate every 10 minutes of mute button.

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7. SAFETY SWITCH
It is the safety device to prevent the heater from overheating when the
temperature controller is malfunctioning.
■ Set the Safety S/W higher than setting point.
■The Safety S/W has wide deviation
■ Safety S/W is the safety device for preventing the heater to
overheat when TEMP. CONTROL is malfunctioning.
8. ROLLER OPERATION
8.1 Connecting the Roller
After cleaning the surface of chamber or separating the roller, please connect the roller with the shaft
correctly.
Axis of rotation
Connected with Motor Shaft of roller Correct connection
8.2 RPM & TIME Set up
RPM setting range is from 2～30rpm, and time range is from 00.00 ~ 99hours 59minutes.
RPM setting change can be done by UP(∧), DOWN(∨) button and possible to change setting value
during running.
When the roller stops by power failure or is turned off and on by user’s mistake, then RPM shows by
a message of n _ _ _ on display.
(RPM is based on the number of roller rotating.)
<RPM setting>
①Press RPM/TIME button to have RPM mode on display (r _ _ _)
②Press SET button for display of (r _ _ _) flickering.
③Press UP(∧), DOWN(∨) button to change the RPM value.
④Press SET button for saving it then rSAVE and RPM value message will appear.
⑤Then press START/STOP button to run it.
User can change RPM value during its operation by UP(∧), DOWN(∨) button and with no saving a
new value.

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<TIME setting>
①Please STOP the product first.
②Press RPM/TIME button and get TIME MODE DISPLAY( t 00.00 ).
③Press SET button then first 2 digits of DISPLAY TIMER will be flickering.
④Set the desired time of hour by UP(∧), DOWN(∨) button.
⑤Press SET button once more, then last 2 digits of DISPLAY TIMER will be flickering.
⑥Set the desired time of minute by UP(∧), DOWN(∨) button.
⑦Press SET button to save then t SAVE on display will appear.
⑧Start operation by START/STOP button and it will stop after set time is over with END message
on display.
9. HOW TO DETACH THE ROLLER
Note : Make sure to turn off the product first prior to disconnect the roller from the product.

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10.TROUBLESHOOTING
Fault description
Possible cause
Recommended fix
Heating
Chamber heating permanently,
set point not held
SSR relay defective
Replace SSR relay
Control panel display defective
Replace display screen
Temperature sensor defective
Replace temperature sensor
Chamber does not heat up.
SSR relay defective
Replace SSR relay
Power not supplied to heating
circuit
Reconnect the power plug on
the panel.
Contact N-BIOTEK service
Unit does not switch on
(main switch is in position “l”
The miniature fuse has blown.
Replace the fuse with type
5x20mm, 220V(5A),110V(7A).
If the newly inserted fuse
triggers again, there is short
circuit: contact N-BIOTEK
service.
Switch defective
Replace the switch
Gas
CO2 concentration in chamber
is too high/ too low.
Defective function of the CO2
controller
Reset the alarm.
CO2 sensor system defective
Contact N-BIOTEK service.
The concentration of CO2 or
O2 does not reach the
adjusted set value.
Gas inlet defective
Replace the gas tube
Gas leaking from inner tube
connecting region.
Replace the inner tube.
Solenoid valve defective.
Replace solenoid valve
Humidity
Condensations inside the
chamber
Fan defective
Replace the fan.
(contact N-BIOTEK service)
Condensation on the door
Improper temperature
distribution b/w the door and
the chamber
Increase the value of door
heating temperature. (Ch.2)
No or too low humidity inside
Water pan empty
Fill the water pan with distilled,
sterile water.
Roller
Not running roller
Main board defective possibly
Replace main board
Not proper engaged with axis
and shaft
Make sure to fix both axis and
shaft in.
Manufactured By : N-BIOTEK, Inc.
402-803 Techno-park, 655 Pyeongcheon-ro, Bucheon si
Gyeonggi Province, Korea
Tel : [phone] E-mail : [e-mail]