Axygen AxySpin R C2500-R-AXY User manual

AxySpin R
Refrigerated Microcentrifuge
Operations Manual
Version 1.0

Table of Content
1. Safety Precautions....................................1
1.1. Operation Safety Precautions.............1
2. General Description..................................2
2.1. Features .............................................2
3. Getting Started..........................................2
3.1. Unpacking...........................................2
3.2. Initial Operation...................................2
3.3. Opening and Closing the Lid...............3
3.4. Loading the Rotor ...............................3
3.5. Starting and Stopping a Run ...............4
3.6. Emergency Lid Release......................4
4. Overview....................................................5
5. Operation...................................................7
5.1. Setting the Time (TIME)......................7
5.2. Setting the Speed (RPM) ....................7
5.3. Setting the Centrifugal Force (RCF)....7
5.4. Setting the Temperature (TEMP).........7
5.5. Cooling ...............................................8
5.6. Quick-Spin Operation..........................8
5.7. Changing the Settings During a Run...8
6. Service and Maintenance.........................8
6.1. Centrifuge Service ..............................9
6.2. Cleaning the Centrifuge ......................9
6.3. Cleaning the Rotor..............................9
6.4. Disinfection.........................................9
6.5. Replacing the Rotor............................9
6.6. Replacing fuses ..................................9
7. Troubleshooting......................................10
Appendix A: Technical Specifications..........11
Appendix B: Warranty....................................12
Axyspin R Refrigerated Microcentrifuge

1. Safety Precautions
Before using the AxySpin R refrigerated microcentrifuge for the first time, please read this entire
operating manual carefully. To guarantee problem free, safe operation, it is essential to observe the
following points:
1.1.Operation Safety Precautions
When using infectious, radioactive, toxic and other solutions which may pose health risks, please
observe the appropriate safety precautions.
Do not use this machine in a potentially explosive environment or with potentially explosive
chemicals.
Install the machine in location free of excessive dust.
Avoid placing the machine in direct sunlight.
Choose a flat, stable surface capable of withstanding the weight of the machine.
Install the machine in the room temperature 15~30°C, relative humidity 20~80%.
Don’t block the air vents.
Make sure the power source conforms to the required power supply specifications.
To avoid electric shock, make sure the machine is plugged into a grounded electric outlet.
Do not allow water or any foreign objects to enter the various openings of the machine.
Switch off the power switch before cleaning or performing any service on the machine, such as
replacing the fuses.
The rotor and the rotor lid must always be installed securely on the motor shaft.
Always load the rotor symmetrically. Each tube should be counterbalanced by another tube
of the same type and weight.
To guarantee sufficient ventilation, ensure that the centrifuge has at least 30 cm of free
space on all sides, including the rear.
Repair should be carried out by Axygen Scientific' authorized service personnel only.
Use original spare parts and accessories only.
Warning Label: Please be aware of the danger of electric shock or other dangers.
AxySpin R™ Refrigerated Microcentrifuge 1

2. General Description
The AxySpin R refrigerated microcentrifuges is a compact, bench-top centrifuge which is suitable for
both research and clinical laboratories. The motor is brushless and requires no routine maintenance.
It is supplied with a 24 x 1.5/2.0 ml rotor for micro samples. The maximum speed of AxySpin R is
13,500 rpm (17,135 rcf). The temperature range is from –10°C to 40°C.
2.1.Features
Brushless motor drive, control speed up to 13,500rpm/17,135xg
Powerful refrigeration system, maintain 4°C at max. speed
Includes unique easy access rotor (24 x 1.5ml)
Exceptionally quiet and compact
Optional StripSpin adapter available for 0.2ml tubes and strips
Compact, ergonomic design
User-friendly operation interface.
Quick button for instant run without setting
Low noise level
Complies with CE, RoHS
3. Getting Started
3.1.Unpacking
The centrifuge is delivered in an external carton and an internal carton with protective PE foam
cushions. Remove the centrifuge from each carton. All packaging should be retained until it has
been established that the centrifuge is working properly.
Open the AxySpin R refrigerated microcentrifuge package and confirm that all items are included:
AxySpin R unit with a 24 x 1.5/
2ml
rotor and a rotor lid
Operation manual

Electric fuses (7A &3.15A)

Warranty card

Rotor wrench

Power cord
If there are any items missing, damaged, or not according to your order, please contact your
distributor or sales representative immediately.
Note: Please use 7A fuse in power source 100~120/50~60Hz countries, while 3.15A fuse is for power
source 200~230V/50~60Hz countries.
3.2.Initial Operation
Place the machine on a stable, flat table. Please keep the machine at least 30 cm from the
surrounding area or objects.
2

Mains power and frequency “MUST”be compatible with the information given on the product label on
the rear of the centrifuge.
Connect the power cord to the power socket of the centrifuge, then to the power source. Switch the
Power On/Off switch to turn on the centrifuge. After the LCD display is on and the buzzer beeps twice,
the centrifuge is ready to operate.
3.3.Opening and Closing the Lid
The lid can be opened only when the centrifuge is powered on. Press button to open the lid.
The lid will be locked automatically when the user presses down the front edge of the lid.
Note: Do not slam the lid!
3.4.Loading the Rotor
For safety reasons, the rotor must be loaded with centrifuge tubes symmetrically. The difference in
the weight between the tubes should not exceed 0.1 gram.
The centrifuge tubes should be loaded in pairs opposite each other and each should contain roughly
the equal weight of sample. One or two additional loaded tubes may need to be added to achieve this.
Please refer Figure 1 to see an example of a properly loaded rotor.
Attention: To reduce air friction and noise, the rotor lid should be attached to the rotor during
centrifugation. Make sure that the rotor lid snaps securely into place, by pressing down on
center catch.
Figure 1. Loading the rotor
Securing
screw
AxySpin R™ Refrigerated Microcentrifuge 3

3.5.Starting and Stopping a Run
Please refer Chapter 5 Operation to set up your preferred settings of time, speed and temperature.
To start a run, press down the (START/STOP) knob.
When the pre-set time expires, the centrifuge will stop automatically. To stop the centrifuge prior to
the expiration of set time, press the (START/STOP) knob again.
When the time is set as “continuous”( - - ), the centrifuge will run continuously. To stop the
continuous run, press the (START/STOP) knob.
By pressing and holding the button, the centrifuge will continue a short-run of set speed. The
short-run will stop if the button is released.
Note:
1. For safety reasons, the (START/STOP) knob will be inactivated when the lid is open. A
symbol “” will be flashing until the lid is closed.
3.6.Emergency Lid Release
When the lid can’t be opened by pressing the button, users can open the lid manually. First, turn
off the power of the centrifuge. Second, use a flat-head screw driver or other tool to remove the
Emergency Lid Release Plug ( Please refer Figure 2 ) on the right-hand side of the machine. Third,
pull the wire (attached to the plug) while gently pushing downward on the lid. The lid will now
release.
4

4. Overview
This section presents an overview of the AxySpin R microcentrifuge’s various components and
control panel (see Figure 2) as well as the symbols and indicators on the LCD display (see Figure 3).
Figure 2. AxySpin R refrigerated microcentrifuge overview
Figure 3. LCD display panel
Keypad Function
Button Symbol Function Description
START/STOP
and
Value
Adjustment
Knob
Start/Stop a run by pressing
down

Rotate to Increase/decrease the
values of time, temperature,
speed
or g force
TIME button To adjust run time setting
RPM button
To adjust speed setting
RCF button
To adjust RCF setting
Air vents
Power On/Off switch
& fuse box
Emergency lid
release plug
Lid lock hole
START/STOP
knob
AxySpin R™ Refrigerated Microcentrifuge 5

TEMP button
To adjust temperature setting
LID button
Open the lid
QUICK button
Quick-Spin centrifugation
Audible Signals
The audible signals are beneficial when you are familiarizing yourself with the operating procedure of
the AxySpin R refrigerated microcentrifuge. They can also be disabled if desired by pressing
button six times. The audible signal function can be resumed by pressing button six times again.
Definition of the audible sounds are shown in the table below.
Audible Sound
Operation
2 beeps
Power on machine
3 beeps
End of a run
6

5. Operation
Users can pre-set the time, temperature and speed (RPM/RCF) before a run.
Attention: Never attempt to operate the centrifuge with rotors or adapters that show signs of
corrosion or mechanical damage. Never centrifuge strongly corrosive materials that may
damage the rotors, accessories or bowl of the unit.
5.1.Setting the Time (TIME)
Operating time can be selected from 0.5 min to 99 min or “continuous”. Press button to change
the time setting. The 2 digits of TIME on the display will flash. Turn the (START/STOP) knob to
increase or decrease the value. Press button again to confirm the selection or press
(START/STOP) knob to store and run the selection. The flashing of time value will stop and the
time value will be stored automatically if no button is pressed after 6 seconds of the adjustment.
Operating time can be set as “continuous” by turning the (START/STOP) knob clockwise or
counter-clockwise to make the 2 digits of TIME to display “- -“. When the time is set as “Continuous”,
the centrifuge will continue to run without a stop. Press (START/STOP) knob to end the
continuous operation.
5.2.Setting the Speed (RPM)
The speed (RPM) can be selected from 500 to 13,500 rpm. Press button to change the speed
setting. The 5 digits of speed (RPM) on the display will flash. Turn the (START/STOP) knob to
increase or decrease the value. Press button again to confirm the selection or press
(START/STOP) knob to store and run the selection. The flashing of RPM value will stop and the
RPM value will be stored automatically if no button is pressed after 6 seconds of the adjustment.
5.3.Setting the Centrifugal Force (RCF)
The speed can be selected from 20 to 17,200 x g, too. Press button to change the RCF setting.
The 5 digits of RCF on the display will flash. Turn the (START/STOP) knob to increase or
decrease the value. Press button again to confirm the selection or press (START/STOP)
knob to store and run the selection. The flashing of RCF value will stop and the RCF value will be
stored automatically if no button is pressed after 6 seconds of the adjustment.
5.4.Setting the Temperature (TEMP)
The temperature can be selected from -10 to 40°C. Press button to change the temperature
setting. The 2 digits of temperature on the display will flash. Turn the (START/STOP) knob to
increase or decrease the value. Press button again to confirm the selection or press
(START/STOP) knob to store and run the selection. The flashing of TEMP value will stop and the
value will be stored automatically if no button is pressed after 6 seconds of the adjustment.
AxySpin R™ Refrigerated Microcentrifuge 7

5.5.Cooling
The temperature can be set from -10 °C and + 40 °C. The set temperature can also be changed
during the run.
Pre-Cooling
Pre-Cooling starts a temperature-control run at 6,000RPM. It allows the rotor to be cooled
down to the new, pre-set temperature quickly. Cooling down from room temperature to 4
°C takes approximately 8 min. (max. 16 minutes.) To activate Pre-Cooling function, press
and hold button, then press (START/STOP) key. Both “RPM” and “TEMP” icons
will flash during Pre-Cooling. The Pre-Cooling can be stopped by pressing the
(START/STOP) key. The Pre-Cooling will function only when the set temperature is
lower than the ambient temperature.
Standby Cooling
When the centrifuge is powered on, the refrigeration system will cool down the chamber to
the pre-set temperature before or after the run if the chamber temperature is above the
pre-set temperature.
Note:
1. To extend the life cycle of the compressor, always remember to inactivate the Standby
Cooling (by increasing the set temperature above room temperature) or power off the
centrifuge when it is not in use.
2. When the lid is open, the Standby Cooling will stop after 3 minutes.
5.6.Quick-Spin Operation
The centrifuge can be operated for a short run by pressing and holding the button. The centrifuge
will continue to run as long as the button is depressed and the time, in seconds, will count up on
the time display. The Quick-Spin speed and temperature is based on the last run settings remaining in
the memory. The icon “RPM” will flash during Quick-Spin operation.
5.7.Changing the Settings During a Run
Users can change the settings while the rotor is running. Through a one-time pressing of any function
buttons ( , , , ) , the current value will switch into the pre-set value mode.
The to-be-changed value flashes, and can then be changed. Once the display switches into the
current value mode after completion of the entered values, the new values are activated.
The new values are stored and executed by pressing the function button again or by waiting 6
seconds.
6. Service and Maintenance
Always unplug the power plug before service and maintenance.
8

6.1.Centrifuge Service
The brushless motor in the AxySpin R requires no routine maintenance. Any required service should
be performed by authorized, qualified personnel only. Repairs performed by unauthorized personnel
may void the warranty.
6.2.Cleaning the Centrifuge
Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories clean. All parts should
be wiped down periodically with a soft cloth. For more thorough cleaning, use a neutral cleaning agent
(pH between 6 and 8) applied with a soft cloth. Excessive amounts of liquid should be avoided. Liquid
should not come into contact with the motor. After cleaning, ensure that all parts are dried thoroughly
by hand or in a warm air cabinet ( Maximum temperature: 50°C).
6.3.Cleaning the Rotor
The rotor should be cleaned after each use. When spinning samples containing phenol or phenol
chloroform, the rotor should be cleaned immediately after use
6.4.Disinfection
Should a spill of infectious materials occur within the rotor or chamber, the unit should be disinfected.
This should be performed by qualified personnel with proper protective equipment.
6.5.Replacing the Rotor
The AxySpin R comes complete with a standard 24-place rotor installed. To remove the rotor, remove
the rotor securing screw from the motor shaft by turning the screw, using the rotor wrench on two
holes of the securing screw (Please refer Figure 1). Lift the rotor directly upward in a straight vertical
motion.
To replace rotor, first make sure the motor shaft and rotor mounting hole are clean. Place the rotor on
the motor shaft. Reinstall the rotor securing screw on the motor shaft by turning it clockwise. Hold the
rotor with one hand and tighten the rotor securing screw, using the rotor wrench.
6.6.Replacing fuses
Check fuse when it is recommended in the Technical Specifications of this manual. The fuse holder is
located in the power inlet on the right-hand side of the unit. Disconnect the power cord from the power
inlet. Open the fuse holder drawer by inserting a small screwdriver under the tab and prying it open.
Remove the innermost (operative) fuse from its retaining tabs and replace the fuse if necessary. A
spare fuse is located in the outer most chamber of the fuse drawer. Replace only with a fuse of the
exact same value as the original.
AxySpin R™ Refrigerated Microcentrifuge 9

7. Troubleshooting
To ensure the product’s quality and performance, the AxySpin R refrigerated microcentrifuge is 100%
inspected by the manufacturer. Error messages will appear on the display if the AxySpin R
refrigerated microcentrifuge fails to perform the attempted action properly. In the case of Error (Err)
messages or faults, please refer to the solutions in the following table to clear the error messages or
faults.
If the following solutions are not able to resolve the problem, please contact Axygen Scientific Inc.’
Service Department to arrange for authorized service.
Symptom
Possible Cause
Solution
“ ” Error Press (START/STOP)
knob when Lid is open.
Close the lid
Defective lid lock
sensor
Call for service
“ ” Error
(rotor
imbalance)
Tubes are not inserted
symmetrically in rotor
holes
Load tubes
symmetrically
Sample liquid in tubes
not properly balanced Make sure that the same volume of
liquid is in each tube
Defective or improperly
adjusted balance
sensor
Call for service
Rotor is stuck when a
run starts
Call for service
Centrifuge
cannot be
started,
although power
is on
Lid not closed correctly
Close lid correctly
No speed or time has
been selected Set speed and/or time
Centrifuge will
not start No power supply Check power source
Check the power
cord connection
Check the power
cord quality
Blown fuse Check the fuse and
replace if necessary
Lid Lock will
not release Defective lid lock Open manually and
have unit serviced
No power from PC
board
Call for service
Lid lock jammed
Call for service
10

Centrifuge is not
receiving power
See “Centrifuge will
not start”
Appendix A: Technical Specifications
Model C2500-R-AXY
Max. Speed 13,500 rpm
Max. RCF 17,135 g
Acceleration Time < 17 seconds
Deceleration Time < 18 seconds
Temperature Range –10°C to 40°C
Max. Rotor Capacity 24 x 2.0/1.5ml tubes
Rotor Lid Easy snap-on type
Time Range 0.5 to 99 min. or “continuous”
Admissible Sample Density 1.2Kg/ml
Noise at Max. Speed 56 dB
Operating Temperature 5°C to 30°C
Operating Humidity (RH) 20~80%
Power Supply
100V/50~60 Hz, 6.0A
110V/ 50~60 Hz, 5.5A
230V/ 50~60 Hz, 2.8A
Dimensions (W x L x H) 280 x 430 x 248 mm
Weight (with Rotor) 22 Kgw
Certification Complies with CE, Class B (EN60101-1-2, EN 55011)
Complies RoHS
Note:
1. Specifications are subject to change without prior notice
AxySpin R™ Refrigerated Microcentrifuge 11

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© 2014 Corning Incorporated. All Rights Reserved.
Declaration of Conformity
Number: CE 00502
Manufacturer: Labnet International, Inc, 31 Mayfield Ave., Edison, NJ 08837
Labnet International declares that the devices described below are in
conformity with the EC directives listed. In the event of unauthorized
modification of any of the devices listed below, this declaration becomes
invalid.
Device Name: AxySpin R Refrigerated Microcentrifuge
Device Models Numbers: C2500-R-AXY
Relevant EC Directives: Low Voltage Directive 2006/95/EC
EMC Directive 2004/108/EC
WEEE 2002/96/EC
RoHS Directive 2002/95/EC
Harmonized Standards EN 61010-1 :2010
EN 61010-2-020:2006
EN 61326-1:2006
EN 55011:2007
EN 61000-3-3:1995+A1:2001 + A2:2005
EN 61000-4-2:1995 + A1: 1998 + A2:2000
EN 61000-4-3:2006
EN 61000-4-4:2004
EN 61000-4-5:2005
EN 61000-4-8:1993 + A1:2000
EN 61000-4-11:2004
Date: July 1, 2012
____ __________________________
Peter Will
Product Line Manager

Axygen Scientific.
33210 Central Avenue
Union City, CA 94587 U.S.A.
TEL: [phone]
FAX; 510-494-0700
EMAIL: [e-mail]
P/N: 401-lrmlab-10, 2008/8
AxySpin R™ Refrigerated Microcentrifuge 13