NanoEnTek ADAMII-CD34 User manual

User manual
Bench-top fluorescence cell counter
-CD34

2
All the materials in this user manual are protected by Korean and international copyright laws.
They cannot be reproduced, translated, published or distributed without the permission of the
copyright owner.
Every effort has been made to ensure that all the information contained in the ADAMII™ User
Manual is correct at the time of printing. However, NanoEntek Inc. reserves the right to make any
changes necessary without notice as part of ongoing product development.
ADAMII™ User Manual
Website: www.nanoentek.com
E-mail: [email protected]
Manufactured by NanoEntek, Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone] Fax. [phone]
MT Promedt Consulting GmbH
Altenhofstrasse 80, 66386 St. Ingbert, Germany
NanoEntek America, Inc.
220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA
Tel. [phone] Fax. [phone]
Copyright © NanoEntek Inc. All rights reserved.
Published in Korea.
Documentation: NESMU-ACD34-001E (V.1.0)
Revision history: V.0.0 Date: MAR 2017
V.0.5 Date: AUG 2020
V.1.0 Date: MAY 2022

ADAMII™ © NanoEntek, Inc. 3
Introduction .................................................................... 4
Product components ..................................................... 5
Product description ....................................................... 6 ~ 7
Installation ...................................................................... 8 ~ 9
Home menu .................................................................... 10 ~ 11
Calibration ...................................................................... 12 ~ 19
Measurement ................................................................. 20 ~ 33
Data …….......................................................................... 34 ~ 43
Setting ………….............................................................. 44 ~ 48
Cleaning & Maintenance ............................................... 49
Troubleshooting ............................................................. 50 ~ 51
Error code ....................................................................... 52 ~ 53
Warranty ......................................................................... 54
Safety precautions ......................................................... 55
Consignes de securite ................................................... 56
Safety symbols ............................................................... 57
Product specifications .................................................. 58
Ordering information ..................................................... 59
Technical support .......................................................... 60
Table of contents

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ADAMII-CD34 is an image-based bench-top cell counter that quantifies CD34+ cells.
ADAMII-CD34 is a FDA 510(k) cleared instrument that can be used for mobilized peripheral blood (MPB)
and hematopoietic progenitor cells apheresis (HPC-A) samples.
ADAMII-CD34 is a CE approved instrument that can be used for mobilized peripheral blood (MPB) and
hematopoietic progenitor cells apheresis (HPC-A) samples.
ADAMII-CD34 reports absolute counts of total and viable CD34+ cells, absolute counts of total and
viable CD45+ cells, and % of viable CD34+ cells in viable CD45+ cells.
[Features]
• Easy to use and maintain
• Accurate and precise measurement
• 4 channels (one BF + 3 FL)
• Automatic stage to capture 75 images
Introduction

ADAMII™ © NanoEntek, Inc. 5
ADAMII-CD34 consists of the following components.
If any of the components is missing or damaged, please contact your local sale representative or send
an email to [email protected].
Product components
ADAMII™ CD34
1 EA
User manual
1 EA
Focus guide
1 EA
4 pcs/1 SET
Power cord with 4 adaptor cords
(for U.S./Canada/ Taiwan/Japan, Europe or UK)
1 EA
USB connection cable
Laptop pre-installed with
ADAMII-CD34 software
(Please note: Laptop will be
packaged separately.)
1 set

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Product description
Front view
Slide holder
Slide door
State LED
Blue LED lights up when the instrument is
connected to the laptop and the ADAMII-CD34
software is running.
State LED
Where one can access the Slide holder.
Where ADAMII-CD34 Assay Slide is inserted to.Slide holder
Slide Door

ADAMII™ © NanoEntek, Inc. 7
Product description
Rear view
To connect USB cable to communicate
with the laptop
To turn power On and Off
To connect the power adapter
USB 3.0 port
Power supply port
Power switch
Power
supply port
Power switch USB 3.0 port
USB 3.0 port

8
Installation
Unpacking
Connect cables
1. Open the box and remove the foam protector.
2. Carefully lift the instrument with two hands.
3. Place the instrument on a flat, stable and level surface.
1. Plug the power cord into an electrical outlet and plug the DC output jack to
the power supply port.
2. Connect USB 3.0 cable to the USB ports in the ADAMII-CD34 and the
laptop.
2. Connect the laptop and the ADAMII™ using two USB cables.
2. Connect the laptop and the ADAMII™ using the USB cable.
Power
supply port
Power switch USB 3.0 port
USB 3.0 port

ADAMII™ © NanoEntek, Inc. 9
Run ADAMII
CD34 software
Installation
1. Turn on the laptop and the ADAMII-CD34.
2. Double-click ‘ADAM’ icon on the laptop to start the ADAMII-CD34
software.
CAUTION:
• The instrument must be turned ON before starting ADAMII-CD34
software in the laptop.
If the instrument is not on when the software starts, the software
will give a warning after searching the instrument for more than
10 seconds.
☞
Note: When it is turned on, the ADAMII-CD34 will be ready to use after
a quick initialization.
☞ Note: When the ADAMII-CD34 is connected to the laptop and
the ADAMII-CD34 software is running, the state LED will
light up.

10
Home menu
Log in
User
management
At the ‘Home’ screen, enter the user ID and password to log in.
Default ID and password are both ‘master’.
Click ‘User management’ when you need to manage accounts (e.g.
to add a new user or to change password). To create a new ID,
fill out the blanks (user name, user ID, password, confirm PW) and
click ‘Add’.
☞ Note: Only the master user can access the ‘User management’ .

ADAMII™ © NanoEntek, Inc. 11
Home menu
Audit trail Click ‘‘Audit trail” to search and trace detailed information of each
user’s activities.
1. Enter the User ID and set the date that you want to search.
2. Click ‘Search’ button.
3. The record of the selected user is displayed.
☞ Note: Only the master user can access the ‘’Audit trail’ .

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Calibration
1. After turning on the ADAM II CD34 first time, the instrument needs
to be calibrated. Subsequently, it is recommended that the calibration
is done regularly or at least once a week.
A pop-up window for calibration appears as shown below.
Calibration
procedure

ADAMII™ © NanoEntek, Inc. 13
Calibration
Calibration
procedure
2. Shake tube labeled with ADAMII Calibration Beads vigorously or vortex
briefly for 5 seconds before use.
☞
Note: ADAMII Calibration Beads are included in ADAMII-CD34 kit.
Before use, the ADAMII Assay Slide should be removed from
refrigerator and kept at room temperature for at least 30 minutes.
The reagents should be kept at 4ºC.
3. Once again, vortex for 4 seconds and pipette 25uL immediately.
4. Load the Calibration Beads into an ADAMII Assay Slide.
[Shake bottle vigorously] [Vortexing]
4 sec.
[Vortexing]
[Loading volume: 25 uL]
[Pipetting Calibration Beads]
5 sec.

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Calibration
Calibration
procedure
Warning:
• Calibration Beads loading error:
- Ensure that calibration bead solution is fully loaded into slide.
- If loading volume is not enough, calibration can be inaccurate.
CAUTION:
• When loading samples to the slide, please release sample gently
and slowly to avoid bubbles because bubbles interfere image analysis.
5. Leave for 20 seconds (Calibration Beads needs time to settle).
6. To insert the Assay Slide, open the slide door and press the black
bar in the slide holder. Once the cover of the slide holder pops
open, insert the Assay Slide all the way in.
For detailed steps, see page 21.
[Low volume] [Correct volume]
20 sec.

ADAMII™ © NanoEntek, Inc. 15
CAUTION:
• Lot number has to be entered after inserting slide.
Calibration
Calibration
procedure
7. Enter lot number of Calibration Beads.
8. Select ‘Bright’ channel and adjust the focus using the focus buttons.
To view beads 2x bigger, press ‘Zoom in’ button.

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Calibration
Calibration
procedure
[Focusing example - representative images]
• Bright - Good Focus: Compared to gray background, dark inside
of beads and bright boundaries around beads.
• Bright - Bad Focus: bright inside of beads or blurry boundaries
around beads.
• FL - Good Focus: Compared to dark background, white inside of beads
and crisp boundaries around beads.
• FL - Bad Focus: gray inside of beads and fuzzy boundaries.
Good Focus VS. Bad Focus
☞ Note: Please check ‘ADAMII™ Focus guide’ before setting the focus.
9. Press the ‘Save BR Focus’ after adjusting focus.
* Enlarged image
Bright- Bad Focus
Bright- Good Focus
* Enlarged image
FL- Bad Focus
FL- Good Focus

ADAMII™ © NanoEntek, Inc. 17
Calibration
Calibration
procedure
11. Press the ‘Save FL Focus’ after adjusting focus.
10. Select FL3 channel and adjust the focus using the focus button.
To view cells 2x bigger, press the ‘ZOOM IN’ button.
12. Press the ‘Run’ button.
☞ Note: After saving the focuses of “Bright” and “FL3”, the focuses
of the other channels (FL2 and FL1) will be automatically
adjusted.
3~4 min

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Calibration
Calibration
procedure
13. When the calibration is completed, the results will be displayed.
14. You can see the previous record of calibration by clicking “Record”
button.
☞
Note: If any of the results are “FAIL”, please contact the manufacturer
or distributor.
☞ Note: If any of the results are “Low”, please check the focus or
expiration date of the Calibration Beads. Or please re-test
using another slide.

ADAMII™ © NanoEntek, Inc. 19
Calibration
Calibration
procedure
15. The calibration results are displayed. To save the results, click
‘Save’ button.
16. Press “Close” button to finish calibration.

20
Measurement
Sample
preparation
Load sample
Prepare sample following the instruction enclosed in ADAMII-CD34
kit.
To measure a sample, click ‘Measure’ menu.
Load 25 uL stained sample into an ADAMIITM Assay Slide.
[Load 25μL sample]
☞ Note: Please refer to page 14 to check cautions for loading correct volume .
☞ Note: Before loading the stained sample or Calibration Beads to
the Assay Slide, Please mix the sample thoroughly.
(e.g. vortex for a few seconds).