Axygen MAXYGENE II User manual

DOMINIQUE DUTSCHER SAS

DOMINIQUE DUTSCHER SAS

patent owned or controlled
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DOMINIQUE DUTSCHER SAS

2.1 FEATURES........................................................................................................................2
2.2 PRODUCT OVERVIEW.......................................................................................................2
3.1 UNPACKING.....................................................................................................................6
3.2 INITIAL OPERATION .........................................................................................................6
3.3 HEATED LID ....................................................................................................................6
3.4 LID OPENING/CLOSING ...................................................................................................7
3.5 FUNCTION MAP .............................................................................................................10
3.6 KEY ENTRY SYSTEM ..................................................................................................... 11
3.7 LOADING THE REACTION VESSEL.................................................................................. 11
4.1 VIEWING A USER FOLDER .............................................................................................12
4.2 CREATING A NEW USER FOLDER ...................................................................................13
4.3 EDITING A USER FOLDER...............................................................................................13
4.4 DELETING A USER FOLDER............................................................................................13
5.1 RUNNING A PROTOCOL ..................................................................................................15
5.2 PAUSE A PROTOCOL .......................................................................................................16
5.3 VIEWING RUNNING PROTOCOL INFORMATION...............................................................16
5.4 TERMINATING A PROTOCOL ...........................................................................................17
6.1 CHANGING TEMPERATURE/TIME VALUES......................................................................18
6.2 INSERTING/DELETING TEMPERATURE SEGMENT ...........................................................19
6.3 CHANGING TEMPERATURE SEGMENT COUNT (TEMP) AND CYCLE COUNT (CYC)..........19
6.4 ADVANCED SETTINGS....................................................................................................19
6.5 SAV I N G A PROTOCOL .....................................................................................................23
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9.1 DATA LOG .....................................................................................................................26
9.2 DATE & TIME ................................................................................................................27
9.3 LID TEMPERATURE ........................................................................................................27
9.4 BEEPER .........................................................................................................................28
9.5 ADMINISTRATOR............................................................................................................28
9.6 SERVICE MODE..............................................................................................................28
9.7 RAMPING SPEED............................................................................................................28
9.8 TM CALCULATOR ..........................................................................................................29
10.1 CLEANING THE UNIT....................................................................................................30
10.2 CLEANING THE HEATED LID .........................................................................................30
10.3 REPLACING A FUSE......................................................................................................30
10.4 CHANGING THE THERMAL BLOCK ...............................................................................30
1. OPERATING TEMPERATURE ENVIRONMENT MAY BE UNSUITABLE.................................31
2. ELECTRONIC COOLING ELEMENT MAY BE DAMAGED OR AGED.....................................31
11.1 ERROR MESSAGES.......................................................................................................32
DOMINIQUE DUTSCHER SAS

DOMINIQUE DUTSCHER SAS

Before using the MaxyGene II thermal cycler for the first time, please read this entire
operating manual carefully. To guarantee problem free, safe operation of the MaxyGene II
thermal cycler, it is essential to observe the following points.
1. Do not use the machine in a potentially explosive environment or with potentially
explosive chemicals.
2. Install the machine in location free of excessive dust.
3. Avoid placing the machine in direct sunlight.
4. Choose a flat, stable surface capable of withstanding the weight of the machine.
5. Install the machine in the room temperature 15 ~ 30 C, relative humidity 20
~80%.
6. DO NOT block the air vents.
7. Keep the side and rear of the machine at least 30cm from the wall or other
machine.
8. Make sure the power source conforms to the required power supply specifications.
9. To avoid electric shock, make sure the machine is plugged into a grounded
electrical outlet.
10. Do not allow water or any foreign objects to enter the various openings of the
machine.
11. Switch off the machine before cleaning or performing service on the machine,
such as replacing the fuses.
12. Repairs should be carried out by authorized service personnel only.
Please be aware of the heated block.
Please be aware of the danger of electric shock or other dangers.
DOMINIQUE DUTSCHER SAS

The MaxyGene II thermal cycler is a powerful, reliable and affordable thermal cycler. It
is equipped with a gradient function that enables the user to set a maximum 24 C
temperature gradient between the BLOCK 1 to BLOCK 6 (16 well/block).
Easy-to-operate slide and lever type lid which prevents accidental skin burn
Large LCD panel enhances visibility and ease-of-operation.
Graphic display makes programming quick and simple.
Simple and easy to use graphical interface.
Extended Temperature return function that makes touchdown possible
Gradient function enables optimization of annealing temperatures.
Preset protocols that make starting amplification easier.
Air intake vents on the front and sides and exhaust vents on the rear that reduce
heat-based interference with other equipment.
Interchangeable blocks.
Automatically creates operating history and error logs.
Heated lid to prevent the formation of condensation.
Robust and attractive design.
Auto restart after power failure.
The front panel of MaxyGene II thermal cycler includes a display panel and 22-key
keypad which consists of function keys (F1-F5), alphanumeric keys, directional arrow
keys and the key.
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Use the function keys (F1 to F5) to activate the “button” displayed on
the screen above the key; for example, F1 for RUN.
The four Directional Arrow keys can move the cursor into different
directions.
The current selection is activated or accepted when the ENTER key is
pressed.
The alphanumeric keys are usually used as number keys for the entry
of time, temperature, password and cycle numbers. When entering a
user name or protocol name, a small screen will pop out to display the
letters after pressing the Alphanumeric keys. Press the selected key
until the desired letter is highlighted. Press the ENTER key to select
the letter or wait 5 seconds.
Press CLEAR key will erase the entry in an input field.
By pressing STOP, the Protocol current running will be terminated.
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The lid with heating pad is designed to protect against evaporation
resulting from heating of the reaction tube and reaction plate.
Rotate Lid Knob in clockwise will lower the heating plate to place
pressure on the cap of tubes. This forces the tubes to sit firmly in the
block for better contact. Rotate Lid Knob in counter-clockwise
direction until the Lid pops up. Only after the Lid pops, can users
slide the Lid to the rear.
Section of the unit in which sample tubes and plates are placed.
Used to enter the various Protocols and settings.
which includes Display and Key-Pad
Displays current status of the various system features and functions.
for air output
for air output
for air intake
DOMINIQUE DUTSCHER SAS