Axygen MAXYGENE II User manual

DOMINIQUE DUTSCHER SAS

DOMINIQUE DUTSCHER SAS

patent owned or controlled
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DOMINIQUE DUTSCHER SAS

2.1 FEATURES........................................................................................................................2
2.2 PRODUCT OVERVIEW.......................................................................................................2
3.1 UNPACKING.....................................................................................................................6
3.2 INITIAL OPERATION .........................................................................................................6
3.3 HEATED LID ....................................................................................................................6
3.4 LID OPENING/CLOSING ...................................................................................................7
3.5 FUNCTION MAP .............................................................................................................10
3.6 KEY ENTRY SYSTEM ..................................................................................................... 11
3.7 LOADING THE REACTION VESSEL.................................................................................. 11
4.1 VIEWING A USER FOLDER .............................................................................................12
4.2 CREATING A NEW USER FOLDER ...................................................................................13
4.3 EDITING A USER FOLDER...............................................................................................13
4.4 DELETING A USER FOLDER............................................................................................13
5.1 RUNNING A PROTOCOL ..................................................................................................15
5.2 PAUSE A PROTOCOL .......................................................................................................16
5.3 VIEWING RUNNING PROTOCOL INFORMATION...............................................................16
5.4 TERMINATING A PROTOCOL ...........................................................................................17
6.1 CHANGING TEMPERATURE/TIME VALUES......................................................................18
6.2 INSERTING/DELETING TEMPERATURE SEGMENT ...........................................................19
6.3 CHANGING TEMPERATURE SEGMENT COUNT (TEMP) AND CYCLE COUNT (CYC)..........19
6.4 ADVANCED SETTINGS....................................................................................................19
6.5 SAV I N G A PROTOCOL .....................................................................................................23
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9.1 DATA LOG .....................................................................................................................26
9.2 DATE & TIME ................................................................................................................27
9.3 LID TEMPERATURE ........................................................................................................27
9.4 BEEPER .........................................................................................................................28
9.5 ADMINISTRATOR............................................................................................................28
9.6 SERVICE MODE..............................................................................................................28
9.7 RAMPING SPEED............................................................................................................28
9.8 TM CALCULATOR ..........................................................................................................29
10.1 CLEANING THE UNIT....................................................................................................30
10.2 CLEANING THE HEATED LID .........................................................................................30
10.3 REPLACING A FUSE......................................................................................................30
10.4 CHANGING THE THERMAL BLOCK ...............................................................................30
1. OPERATING TEMPERATURE ENVIRONMENT MAY BE UNSUITABLE.................................31
2. ELECTRONIC COOLING ELEMENT MAY BE DAMAGED OR AGED.....................................31
11.1 ERROR MESSAGES.......................................................................................................32
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DOMINIQUE DUTSCHER SAS

Before using the MaxyGene II thermal cycler for the first time, please read this entire
operating manual carefully. To guarantee problem free, safe operation of the MaxyGene II
thermal cycler, it is essential to observe the following points.
1. Do not use the machine in a potentially explosive environment or with potentially
explosive chemicals.
2. Install the machine in location free of excessive dust.
3. Avoid placing the machine in direct sunlight.
4. Choose a flat, stable surface capable of withstanding the weight of the machine.
5. Install the machine in the room temperature 15 ~ 30 C, relative humidity 20
~80%.
6. DO NOT block the air vents.
7. Keep the side and rear of the machine at least 30cm from the wall or other
machine.
8. Make sure the power source conforms to the required power supply specifications.
9. To avoid electric shock, make sure the machine is plugged into a grounded
electrical outlet.
10. Do not allow water or any foreign objects to enter the various openings of the
machine.
11. Switch off the machine before cleaning or performing service on the machine,
such as replacing the fuses.
12. Repairs should be carried out by authorized service personnel only.
Please be aware of the heated block.
Please be aware of the danger of electric shock or other dangers.
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The MaxyGene II thermal cycler is a powerful, reliable and affordable thermal cycler. It
is equipped with a gradient function that enables the user to set a maximum 24 C
temperature gradient between the BLOCK 1 to BLOCK 6 (16 well/block).
Easy-to-operate slide and lever type lid which prevents accidental skin burn
Large LCD panel enhances visibility and ease-of-operation.
Graphic display makes programming quick and simple.
Simple and easy to use graphical interface.
Extended Temperature return function that makes touchdown possible
Gradient function enables optimization of annealing temperatures.
Preset protocols that make starting amplification easier.
Air intake vents on the front and sides and exhaust vents on the rear that reduce
heat-based interference with other equipment.
Interchangeable blocks.
Automatically creates operating history and error logs.
Heated lid to prevent the formation of condensation.
Robust and attractive design.
Auto restart after power failure.
The front panel of MaxyGene II thermal cycler includes a display panel and 22-key
keypad which consists of function keys (F1-F5), alphanumeric keys, directional arrow
keys and the key.
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Use the function keys (F1 to F5) to activate the “button” displayed on
the screen above the key; for example, F1 for RUN.
The four Directional Arrow keys can move the cursor into different
directions.
The current selection is activated or accepted when the ENTER key is
pressed.
The alphanumeric keys are usually used as number keys for the entry
of time, temperature, password and cycle numbers. When entering a
user name or protocol name, a small screen will pop out to display the
letters after pressing the Alphanumeric keys. Press the selected key
until the desired letter is highlighted. Press the ENTER key to select
the letter or wait 5 seconds.
Press CLEAR key will erase the entry in an input field.
By pressing STOP, the Protocol current running will be terminated.
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The lid with heating pad is designed to protect against evaporation
resulting from heating of the reaction tube and reaction plate.
Rotate Lid Knob in clockwise will lower the heating plate to place
pressure on the cap of tubes. This forces the tubes to sit firmly in the
block for better contact. Rotate Lid Knob in counter-clockwise
direction until the Lid pops up. Only after the Lid pops, can users
slide the Lid to the rear.
Section of the unit in which sample tubes and plates are placed.
Used to enter the various Protocols and settings.
which includes Display and Key-Pad
Displays current status of the various system features and functions.
for air output
for air output
for air intake
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Power cable socket and fuse.
Power On/Off switch
Control the brightness of LCD panel. Please use Philip-head screw
driver to adjust it.
For run summary download to computer
For run summary download to computer
For air output
Indicate the model name, serial number, power specification, and
other important information
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Open the MaxyGene II thermal cycler package and confirm that all items below are
included:
If there are any items missing, damaged, or incorrect items, please contact your
distributor or sales representatives immediately.
Note: Please use 7A fuse in power source 100~120/50~60Hz countries, while 3.15A fuse
is for power source 200~220V/50~60Hz countries.
Place the machine on a steady, flat table. Please keep the machine at least 30cm from the
wall or other machine. Connect the power cord to the power socket in the rear of the
machine. Check the power source is compatible with your machine’s fuse rating input.
Press Power On/Off Button to switch on the machine. The screen will show the software
version for 10 seconds and then enter User Folder screen. Pressing any key will go to
User Folder screen immediately.
The Heated Lid heats the air in the upper part of the sample vessels at higher temperature
than the reaction mixture. This prevents condensation of the evaporated water vapor on
the vessel walls, keeping the concentration unchanged during the heating and cooling
cycles. The top also places pressure on the caps of tubes and film on titer plates to prevent
vapor loss and cross contamination of samples.
When using plates it is highly recommended to use a Silicone Compression Mat (Axygen
TC96-CM-10, for 96-well models), to assist in placing even pressure on the plates.
Note:
1. If MaxyGene II is started cold, approximately two to three minutes will be required
before heated lid is at operating temperature.
MaxyGene II thermal cycler unit Operation manual
Electric fuses (7A & 3.15A) AC power cord
Warranty card 0.2 ml Axygen tubes
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2. To avoid heat dissipation, the Heated Lid should be closed and tighten during a
protocol run. Keeping the heated lid open during a protocol run might cause
“HEATER ERROR”, because the heated lid fails to reach the set temperature.
3. To evapo rate the c ondensation water inside sample block, a n d t o avoid “HEATER
ERROR”, users should turn off the Lid Temperature under SYSTEM mode before
heating the sample block and keeping the lid open.
There are two ways to open the lid: 1) Slide the lid to the rear, 2) lift up the lid. The first
method is recommended for common operations and to prevent the skin burn from the
heated lid. The second method is used to clean up the heating plate of the lid.
Rotate Lid Knob in a clockwise direction to lower down the heating plate and press down
on the cap of tubes, tighten until you feel resistance. This adjustment will provide better
contact between the tube or plates and the block for better performance. Rotate the Lid
Knob in counter-clockwise direction as shown in Figure 4 until the Lid pops out. Only
when the Lid pops out, users can slide the Lid to the rear as shown in Figure 5.
To lift up the lid for heating plate cleaning, please refer Figure 6 ~ 8.
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The User Screen includes 5 function options; 1) folders under selected User folder,
2) (Create) a User, 3) a User, 4) a User, 5) . Once a user
folder is selected and the password is approved, users can; 1) a Protocol,
2) (Create) a protocol, 3) a Protocol, and 4) a Protocol.
The function map is shown in Figure 9. Various functions, including Gradient, can be
added to the selected/created Protocol.
Protocol
Protocol
Protocol
Protocol
User User User
User Folder
User
Insert
Delete
Advanced
Optimization
Increment
Pause
Data Log
Date &Time
Lid Temp.
Beeper
Administrator
Service Mode
Ramping Speed
Tm Calculator
Save
Main Menu
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Note that the alphanumeric keys are assigned multiple characters, selectable by repeatedly
pressing the key to cycle through the available characters. The highlighted character or
number will automatically be entered into the name of a newly created or edited Protocol
(or User) after a very short period of time, if users don’t continue to press the key.
0, space, +, =, <, >, &, (, ), %
1, . , , ,-, ?, ‘, #, : , ; ,/
A, B, C, a, b, c, 2
D, E, F, d, e, f, 3
G, H , I , g , h , i , 4
J, K, L, j, k, l, 5
M, N, O, m, n, o, 6
P, Q , R, S, p, q, r, s, 7
T, U, V, t, u, v, 8
W, X, Y, Z, w, x, y, z, 9
For the optimal performance of the MaxyGene II, we recommend Axygen tubes and
plates.
The recommended sample volume for 0.2 ml tubes or 96-well plates is 5-100ul. If using
the heated lid with 96 well plates it is highly recommended to use a Silicone Compression
Mat ( Axygen TC96-CM-10, Axygen CM-96-RD ) to assist in placing even pressure on
the plates.
Note: To avoid deformation of the sample tubes or Strips, it’s important to load at least
one tube in each of the four corners and at least four tubes in the center of the block, this
will distribute the pressure evenly throughout the surface.
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After the unit is turned on, the initial screen which contains software version and
manufacturer’s name will show up for 10 seconds and then enter User Folder screen.
Pressing any key will go to User Folder screen immediately.
A sample screen is shown below.
Users can use the function keys( ) above to view, create, edit and delete the User
folders. is used for system settings.
Note:
1. The [ PRESET ] and [ PUBLIC ] folders are pre-registered folders which require no
Personal Identification Number(PIN) to enter.
2. The [ PRESET ] folder includes some frequently-used Protocols defined by vendor,
while the [ PUBLIC ] folder is open to all users.
All Protocols are stored inside the User folders. Use directional arrow keys to select a
User folder. Press to view the names of all Protocols inside the selected folder.
To view the contents of the User, will require you to enter the 4-digit
F 1 F 2 F 3 F 4 F 5
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Directory User Folder Name Status Bar Date & Time
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as shown in the screen below. After the PIN is entered,
press or key to confirm the PIN. To run a Protocol, please refer
Section 5.1.
Under User Folder screen, press can create a new User folder. After entering the
new User name, press to accept the new User folder name. The MaxyGene
II will prompt you to enter a 4 digit PIN. Pressing will save the new folder
name and PIN.
Under the User Folder screen, use directional arrow keys to select a User folder. Press
to edit the User name. A PIN is required to edit the User folder name. After
editing the User folder name, press to save the edited User name.
Use directional arrow keys to select a User folder. Press to delete the
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F 1 F 2 F 3 F 4 F 5
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selected User folder. You are required to enter the PIN and press or
. The screen will prompt “Do you want to delete”. Press to
confirm the deletion. Press to abort the deletion.
Note: User folders which contain any protocols can’t be deleted except by administrator.
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