BANDELIN Sonoplus HD 2070 User manual

Ultrasonic Homogeniser HD 2070
7Ultrasonic Homogenisers
Instructions for Use
valid for:
HD 2070 Volume: 1 ml - 500 ml
HD 2200 Volume: 2 ml - 1000 ml
51436a GB/2017-10

Copyright & limit of liability
This document may not be reproduced, either in full or in extracts, without prior approval of BANDELIN electronic GmbH & Co. KG,
hereinafter referred to as BANDELIN.
The German-language original is the binding version of this document. Any differences in the translation are not binding and have
no legal effect. In case of any discrepancies between the translation and the original of this document, the original version will take
precedence.
BANDELIN accepts no responsibility or liability for damage caused by improper handling or usage contrary to the intended
purpose.
The documentation has been prepared with great care. Liability for indirect or direct damage arising because of incomplete or
erroneous information in this documentation, as well as its delivery and usage, is excluded.
© 2017
1 2 GmbH & Co. KG, Heinrichstrasse 3 – 4, Germany, 12207 Berlin,
Tel.: [phone] - 0, Fax: [phone], [email protected]
2 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

General
The equipment, the accessories and the preparations are to be used in accordance with the user
instructions and/or the product information.
The instructions are part of the scope of delivery and should be stored in the vicinity of the
device for later reference. This also applies if possession of the device is transferred elsewhere.
Before the device is put into operation, these instructions are to be read carefully and completely
in order for the user to become familiarised with all functions.
The warnings and safety precautions (chapter 1.5) are always to be heeded during use.
The manufacturer will not assume any responsibility for the device's safety or functional ability
in the event of improper handling or usage contrary to the intended purpose. In the event of
unauthorised alterations/modifications, both the warranty claim and the CE conformity will no
longer be valid.
If Service is required, please contact the specialist dealer in charge or the manufacturer.
Symbols used:
Symbol Significance Explanation
Danger Identifies information that could signify a risk to life and limb,
especially through electric shock, if not observed.
Caution
Identifies information that must be observed and adhered to
without fail in order to avoid damage to the device and user.
When device parts are labelled with this symbol, the
documentation must be referred to.
Warning Warning of hot surface.
Important Identifies information that is important for execution.
Note Identifies information provided for explanatory purposes.
In vitro diagnostics
information
Identifies information that is important for in vitro diagnostics
applications.
Medical note Identifies information that is important for medical use.
Do not grip inside For health reasons, touching the oscillating fluid is prohibited.
Wear ear protectors For health reasons, spending long periods of time in the
vicinity of the device without ear protectors is prohibited.
Operating sequence
instructions
Identifies instructions that must be followed in the described
sequence.
3 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

Table of Contents
1 Product description .........................................................................................................6
1.1 Mode of operation ..........................................................................................................7
1.2 Purpose ..........................................................................................................................7
1.3 CE conformity .................................................................................................................8
1.4 Technical data ................................................................................................................ 8
1.4.1 HF Generator (GM) ........................................................................................................8
1.4.2 Technical data, ultrasonic transducers (UW) ................................................................ 10
1.4.3 Remote operation ......................................................................................................... 11
1.4.4 Electromagnetic ambient conditions (EMC) ................................................................. 11
1.5 Warning and safety precautions ...................................................................................12
2 Preparation ...................................................................................................................13
2.1 Scope of delivery ..........................................................................................................14
2.2 Set-up / assembly .........................................................................................................14
2.3 Start-up .........................................................................................................................15
3 Operation ......................................................................................................................15
3.1 Operating elements ......................................................................................................15
3.1.1 Types of operation ........................................................................................................ 16
3.1.2 Power - Amplitude setting ............................................................................................. 16
3.1.3 Advantages of the generator ........................................................................................ 16
4 Use ...............................................................................................................................17
4.1 Notes on use ................................................................................................................17
4.2 General use ..................................................................................................................18
4.3 Selecting a suitable probe ............................................................................................ 21
4.3.1 Mounting and dismounting of the probes .....................................................................22
4.3.1.1 Mounting and dismounting the titanium plates .............................................................23
4.3.1.2 Mounting and dismounting of micro and tapered tips and long
probes ..........................................................................................................................24
4.4 Mounting and dismounting of the standard and booster horns ....................................25
5 Cleaning and maintenance of the homogeniser ...........................................................27
4 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

5.1 Cleaning and care ........................................................................................................27
5.2 Treatment of contaminated parts in the ultrasonic transducer, vessels and accessories
pertaining to the medical field .......................................................................................28
5.3 Warehousing / storage .................................................................................................28
6 Maintenance and repair ................................................................................................29
6.1 Maintenance .................................................................................................................29
6.2 Functional checks .........................................................................................................29
6.3 Error analysis ...............................................................................................................30
6.4 Repairs and service ......................................................................................................32
6.4.1 Decontamination certificate ..........................................................................................32
7 Accessories ..................................................................................................................33
7.1 Required accessories ...................................................................................................33
7.2 Optional accessories ....................................................................................................33
7.3 Preparations - not applicable - ....................................................................................34
8 Consumable materials - not applicable - .....................................................................34
9 Taking the unit out of service ........................................................................................ 34
10 Key words .....................................................................................................................34
Informative appendices
A Options and accessories
B Spanner for mounting/dismounting
C Decontamination - sample copy
5 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

1
3
6
3a
4
5
2
7
GM 2070 and GM 2200
10
12
11
GM 2070-U and GM 2200-U
10a
10b
12
11
1 Product description
The SONOPULS ultrasonic homogeniser is essentially made up of three components: the HF
generator, the ultrasonic transducer, and the working tip (probe). The individual components can
be varied using a multitude of options and accessories. The type specification and serial number
are found on the type plate.
Product features:
• HF generator (1) in low-maintenance, robust plastic housing, with connections for the ultrasonic
transducer.
• Operating and display panel (2)
• Ultrasonic transducer (3) with Start/Stop
key (3a)
• Ultrasonic oscillating system (4)
• Booster horn (5)
• Probe (6)
• Mains switch (7)
Product features on the rear side of the HF generator:
• Depending on type, with mains cable (10) or mains socket (10a) with fuse holder and, if needed,
with a switch (10b) for voltage selection.
• HF socket (11)
• Remote control socket(12)
6 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

1.1 Mode of operation
The HF generator transforms the mains energy input (mains frequency 50 or 60 Hz) into
high-frequency energy with a frequency of 20 kHz. Thanks to the ultrasonic transducer that is
connected to the HF generator, the high-frequency energy of the HF generator is converted into
ultrasound and thus into mechanical energy. This takes place with an efficient and robust PZT
ultrasonic oscillating system. Thus, mechanical deflections with the same frequency of 20 kHZ
are generated on the tip of the probe and transferred into the sonication medium as ultrasound
waves with a high power intensity. The amplitude is held constant by the ultrasonic transducer
(AMPLICHRON® system), independently from the applied load, using a signal feedback as long
as the maximum power allowed is not exceeded. Thanks to these measures, the reproducibility of
the process parameters is guaranteed and validation of the process is supported.
Standard and/or booster horns with probes (micro tips, tapered tips or titanium plates) are
mounted on the ultrasonic transducer. These work as mechanical transformers and enable a
multiple mechanical amplification of the ultrasound amplitude at the tip.
1.2 Purpose
SONOPULS ultrasonichomogenisers generate high-performance ultrasound with high intensities
and ultrasonic amplitudes, which are transferred into liquid media using working tools called
probes. They are used in laboratories, clinics, and in industrial research, and they perform diverse
tasks during sample preparation in Quality Assurance, scientific experiments, analyses, and in
pilot or short-series manufacture.
Application examples:
• Cell disruption for paternity tests
• Cell disruption (extraction of microorganisms, tissue cells)
• Homogenising of liquids
• Emulsifying hard-to-mix liquids
• Dispersion of agglomerates
• Acceleration of chemical reactions
• Degassing of fluids
• Sample preparation in environmental analytics (wastewater tests, soil samples)
• Sonochemistry
SONOPULS ultrasonic homogenisers are also used for in-vitro diagnostic lab procedures, to
procure information from the handling of organic materials with ultrasound. Therefore, they are
classified and treated in accordance with the Directive 98/79/EC on in-vitro diagnostic medical
devices.
7 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

1.3 CE conformity
SONOPULS ultrasonic homogenisers are declared as IVD products and satisfy the CE marking
criteria for the European Directives:
- IVD directive
- Low-voltage directive
- Electromagnetic compatibility directive
in their currently valid versions.
A declaration of conformity can be requested from the manufacturer by providing the serial
number.
1.4 Technical data
SONOPULS ultrasonic homogenisers are interference-free and marked with a (-.
Safety: EN 61010-1,
EMC: EN 61326-1
/
1.4.1 HF Generator (GM)
Generator GM 2070 GM 2200 GM 2070-U GM 2200-U
HF power/2 70 W 200 W 70 W 200 W
HF frequency 20 kHz 20 kHz 20 kHz 20 kHz
Mains connection 230 V~ (± 10 %) 50/60 Hz 115 V~ or 230 V~ (± 10 %) 50/60 Hz
Weight 2.5 kg 5.5 kg
Dimensions
(l × w × h) 257 × 180 × 115 mm 255 × 272 × 115 mm
8 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

Protected against access to
dangerous parts with a wire.
Protected against solid foreign
bodies with a diameter of 1 mm or
larger.
Protected from
dripping water.
Cable length: 2 m
Ultrasound frequency: 20 kHz ± 500 Hz
Frequency control/1: Automatic
Time setting range: 0:00:01 – 99:59 [h:mm:ss] or continuous operation
Ultrasound operating mode: Pulsating or continuous
Amplitude setting range: 10 - 100 % in 1 % steps
Operating elements: Control dial, membrane keypad
Operating data display: LED display, illuminated
Remote control (turn on/off): Button on the ultrasonic transducer, potential-free contact,
foot switch TS 8 (optional)
Degree of protection: IP 41
pursuant to DIN EN 60529
Environmental conditions pursuant to EN 61 010-1
Overvoltage category: II
Degree of contamination: 2
Permissible relative humidity up to 31 °C: 80 %
Permissible relative humidity up to 40 °C: 50 %
Permissible ambient temperature: 5 to 40 °C
Condensation not allowed.
Only for indoor operation.
/1 Frequency control:
The HF generator has an automatic search feature for resonance frequency and rate adaptation while in operation. Causes for a change
in the resonance frequency are, for example, a warming of the ultrasonic transducer and the probes, a change in the acoustic load due to
changes in viscosity, and even the mounting of another probe. A frequency drift during operation is corrected by the automatic frequency
control.
/2 HF power:
In the case of amplitude control, the ultrasonic power that is needed for the desired probe amplitude is dependent on the viscosity of the
medium. In order to prevent damage to the HF generator and converter, the power is limited to the maximum permitted peak value. In the
case of highly viscous media, this can also result in the desired amplitude not always being reached.
9 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

Protected against access to
dangerous parts with fingers.
Protected against solid foreign
bodies with a diameter of 12 mm
or larger.
Not
protected
against
ingress of
water.
Specifications for use as a medical device
Name: Ultrasonic homogeniser
UMDNS nomenclature (ECRI / DIMDI): 17-125
Purpose: see chapter 1.2.
Medical device pursuant to
Directive 98/79/EC for in-vitro diagnostic medical devices: Category 5 device (miscellaneous)
Type, model, serial number, year of manufacture: See type plate on the generator for
information
The homogeniser has been inspected pursuant to norms currently in effect and must be installed
and put into operation pursuant to EMC directions; information in this regard is found in the
appendix.
Specifications pursuant to the German Medical Devices Operator Ordinance (MPBetreibV):
Start-up on location, functional check
and personnel training (section 5): not required
Technical safety check (STK) (section 6): no specifications
Technical measurement check (MTK) (section 11): not applicable
e-procurement
e-cl@ss:
HD device classification: 32-08-02-02
HD accessories classification: 32-08-92-03
1.4.2 Technical data, ultrasonic transducers (UW)
UW 2070 / UW 2200
PZT ultrasonic oscillating system: 
Start/Stop key: 
Suitable for continuous
operation: 
Frequency: 20 kHz
Weight: 1.2 kg
Dimensions: Ø 70 × 120 mm
Degree of protection: IP 20 according to
DIN 60529
For environmental conditions, see chapter 1.4.1.
10 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

1.4.3 Remote operation
Several options are available for remote control and remote operation. The most convenient
solution may be selected on the basis of the requirements.
Operating
element Methods Functions Connection
1Key manual Ultrasound operation ON/OFF fixed on the ultrasonic
transducer
2Foot switch foot-operated Ultrasound operation ON/OFF Remote control socket
3 external control signal Ultrasound operation ON/OFF Remote control socket
Remote control socket
Contact assignment and functional description of the remote control socket
Diagram Contact Assignment Function
1
2
3
45
1 Input Ultrasound operation ON
Ultrasound operation OFF
2 n/a
3 Earth
4 n/a
5 n/a
Notes:
• The inputs and outputs may not be connected to other circuits, ground planes, or ground
terminals.
• The connection of power or voltage sources of any type is not permitted.
• When connecting a changeover contact (toggle function) to connector 1, the make time must be
> 100 ms.
1.4.4 Electromagnetic ambient conditions (EMC)
The device was tested to DIN EN 61326-1 for electromagnetic compatibility (EMC) and complies
with the requirements for class B devices according to EN 55011.
It is suitable for use in facilities and areas which are directly connected to a public low-voltage
supply network, e.g. medical laboratory facilities.
11 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

1.5 Warning and safety precautions
General
• Keep the device and accessories out of the reach of children and also of persons who have not
been instructed in their operation by reference to these instructions.
• The use of the device or of its parts on humans or animals is not authorised.
• Keep the HF generator and operating elements clean and dry.
• Do not expose the unit to corroding forces.
• When working with the device, please observe hygiene instructions pursuant to chapter 5.2.
• The connection of any type of power or voltage sources to the signal inputs or outputs is
forbidden.
• Signal wires for foot switches, temperature sensors, etc., may not exceed a maximum length of
3 m.
• All plug connections (such as for ultrasonic transducers, foot switches) may only be plugged or
unplugged while the device is turned off.
• The HF generator and ultrasonic transducer must be transported separately.
• The homogenisers HD 2070 and HD 2200 adhere to prescribed EMC limit values, so that it
can be assumed that the electromagnetic radiation emanating from the units is harmless to
humans. A binding statement for wearers of implants can only be made at the place of work and
together with the implant manufacturer. In case of doubt, information regarding the allowable
electromagnetic exposure level is to be obtained from the implant manufacturer.
Operation
• Observe ambient and set-up conditions, see chapter 1.4.1.
• Determine the local mains voltage before connecting the HF generator, activate the switch if
needed.
• Only connect the HF generator to a grounded socket.
Fuse protection 10 A (main circuit breaker).
• The ultrasonic transducer should only be firmly mounted on the black housing using a suitable
support, e.g. stainless steel stand.
• Before each startup, check that the probe is firmly positioned on the standard or booster horn; if
necessary, tighten the probe (see chapter 4.3.1).
• Do not touch any oscillating parts (e.g. standard or booster horns, micro tips or tapered tips,
titanium plates, ultrasonic transducer) during operation! Damage to health is possible.
• Do not touch the sonication vessels with the oscillating probe - probes and vessels could be
damaged.
• The sonication of liquids generates noise. Suitable accessories, such as a sound proof box,
will minimise such noise considerably. If operating without a sound proof box, wear hearing
protection.
• Warning, risk of splashing!
This is especially the case with small sample quantities and when immersing oscillating probes.
• Do not use combustible solvents in open reaction vessels since the operating safety of
the homogeniser could be compromised. Safe extraction of combustible vapours must be
guaranteed. When using a sound proof box, the vapours cannot escape.
• Before any mounting or dismounting (chapter 4.4) of probes or standard/booster horns as
well as accessories, turn off the device and disconnect the ultrasonic transducer from the HF
generator.
12 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

• Only use the prescribed tool for mounting and dismounting (see appendix B).
• Do not use any bent probes (⇒unstable operation, loss of power).
• Liquids must not penetrate the inside of the ultrasonic transducer.
• Never twist the ultrasonic transducer's black housing toward the aluminium cylinder (ultrasonic
oscillating system). The ultrasonic oscillating system and its electrical connections would be
destroyed as a result.
• Do not operate the device without supervision.
Advice for the medical and laboratory field
• The homogeniser is exclusively intended for use by skilled medical personnel.
• It may generate radio interferences or disrupt the operation of devices nearby. It may be
necessary to take remedial measures such as realigning the device or reconfiguring the
homogeniser or the shield.
• During operation, portable or mobile HF communication systems in the vicinity of the
homogeniser should be turned off - operation may be disrupted.
Damage
• If damage to the homogeniser is detected, do not connect the homogeniser to the mains.
• In the event of defects, disconnect the power plug immediately.
• Repairs are only to be conducted by authorised skilled personnel or by the manufacturer.
• Defective parts must only be replaced with original parts or parts of the same quality!
2 Preparation
Carefully unpack the HF generator, ultrasonic transducer and accessories, and inspect them for
completeness or possible transportation damage. If any damage or defects are found, these must
be immediately notified in writing to the transportation company and to the supplier.
Before operation, the ultrasonic bath should be allowed to stand for 2 hours at its operating
location so that it can adjust to the ambient conditions.
13 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

2.1 Scope of delivery
The scope of delivery will depend on the size of the order. However, it will generally include the
following parts:
1 HF Generator GM .... - see delivery note
1 Ultrasonic transducer UW ...
1 Standard horn or booster horn SH ... (already firmly screwed-in)
1 Probe
1 Instructions for Use manual
Additional accessories according to order – see delivery note
2.2 Set-up / assembly
Place the HF generator atop a firm, level and dry surface. In doing so,
• do not group it or stack it over other electrical or electronic devices,
• do not cover the ventilation holes on the ultrasonic transducer's housing,
• guard against moisture and wetness - risk of electric shock.
During delivery, the standard or booster horn is already firmly screwed to the corresponding
ultrasonic transducer.
Other standard or booster horns are mountable. To do so, see instructions in chapter 4.4.
Screw together the supplied probe with the standard or booster horn, see chapter 4.3.1.
Position the ultrasonic transducer safely and correctly inside the holder.
To do so, affix the ultrasonic transducer in the sound proof box's supporting ring or firmly
mount the ultrasonic transducer on the black housing only, using a suitable support, e.g. a
stainless steel stand.
Connect the ultrasonic transducer to the HF generator.
To do so, insert the plug from the ultrasonic transducer into the HF socket located on the rear
side of the generator, and tighten using the threaded ring. If necessary, see chapter 1.
Verify that the mains switch is in the "0" position.
Before the mains connection, identify the pertinent mains voltage, compare it against the type
plate on the bottom of the generator, and only connect a suitable type to a grounded socket.
230 V~ 50/60 Hz alternatively 115 V~ 50/60 Hz
Note:
• Any contact between the contact pins on the ultrasonic transducer's jack must be avoided since
the piezo elements could generate an electric charge through temperature fluctuations ⇒with a
possibility of slight electric shocks.
14 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

1 Mains switch Turning generator on or off
2 Digital timer Display
3 Pulsation level Display
4 Power display Display
5 Power control dial Used for infinitely variable setting of the amplitude (10 - 100 %) during operation
6 Control light Lit green during operation
7 "Start/Stop" key Starts or stops the entered program.
Optional with button on the ultrasonic transducer or the foot switch.
8 Type "+" / "-": the blinking value increases or decreases by one unit.
Press and hold (> 2 sec):
the blinking value increases or decreases through a fast scroll.
9 "Mode" key Used for step-by-step modification of the time entries - minutes, seconds and pulsation
level - and at the end for saving in countdown or continuous operation.
10 "Reset" key Interrupts the current program
Switches from countdown operation to continuous operation and vice versa.
12 345
109876
2.3 Start-up
Check the positioning of the ultrasonic transducer in the holder.
Check the firm positioning of the probe and, if needed, clean thoroughly before first use.
Connect the HF generator to the mains (grounded socket) and switch on.
Conduct a function test pursuant to chapter 6.2.
3 Operation
3.1 Operating elements
Operation is conducted from the operating and display panel on the front side of the HF generator:
15 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

3.1.1 Types of operation
The device features two types of operation: Countdown operation and continuous
operation
In countdown operation, the remaining time and the pulsation level are adjustable. After
activating the START/STOP key, the button on the ultrasonic transducer, or the foot switch, the set
time starts counting backwards in one-second intervals until it reaches zero – then the ultrasonic
generator turns off.
The maximum time that can be set is 99 min and 59 sec (the setting 00:00 is not possible and is
also not displayed).
In continuous operation, the ultrasound emission can be selected as continuous or pulsed
(see table on page 6, below). After activating the START/STOP key, the button on the ultrasonic
transducer, or the foot switch, the time starts counting forward in one-second intervals until it
reaches 99 min and 59 sec, and continues running by starting again at zero.
The detailed operating steps can be found in the tables "Working in continuous operation" and
"Working in countdown operation" 4.2.
3.1.2 Power - Amplitude setting
The amplitude can be set between 10 % and 100 %, in 1% intervals. Before pressing the Start
key, turn the "Power" regulator all the way to the left. After the start of operation, set the desired
value with the control dial. The amplitude will be continuously measured and displayed. Depending
on the ultrasound-dependent change in the liquid medium, the current display may fluctuate by up
to 5 digits at higher outputs during operation.
3.1.3 Advantages of the generator
• Environmentally friendly due to very low stand-by energy consumption.
• Start - Stop functions also available via remote control through the foot switch as well as
through a button on the ultrasonic transducer.
• Large, bright, easily readable display for time, pulse and amplitude.
• Integrated digital timer for specifi cation / display of the sonication times.
• A functional check for the timer can be called up by pressing the "+" and " " keys simultaneously
(see chapter 6.2).
• Fast and easy setting of the time and pulsation level through fast scrolling.
• The most-recently entered settings are also saved in the event of power failures.
• Automatic test with error display on whether the ultrasonic transducer is connected to the
generator (see chapter 6.2).
• Works with the amplitude-constancy AMPLICHRON® control system.
• Easy operation with a membrane keypad as well as a control dial and toggle switch.
• Integrated digital timer for setting and reading out the sonication times.
• Start - Stop - functions also possible with remote control via foot switch.
• “POWER” ⇒ infi nitely variable amplitude setting.
• “CYCLE” ⇒ adjustable pulsing mode; it is used to divide pulses of 1 sec into work steps and
idle steps. The length of the working pulse can be set in 10 steps.
16 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

Pulsation
level
Work step
(sec) Idle step (sec)
1 0.1 0.9
2 0.2 0.8
3 0.3 0.7
4 0.4 0.6
5 0.5 0.5
6 0.6 0.4
7 0.7 0.3
8 0.8 0.2
9 0.9 0.1
-10
4 Use
4.1 Notes on use
• Do not use any bent probes (⇒ unstable operation, loss of power).
• Do not touch the sonication vessels with the oscillating probe - probes and vessels could be
damaged.
• The recommended immersion depth for probes is 10 - 20 mm, to prevent the aspiration and
mixing-in of air. If mixed-in air is desired, the probe may be immersed just a few millimetres.
In the case of greater immersion depths and/or the sonication of liquids with high viscosities,
the probe is more heavily damped. As a result, it is possible that the pre-set amplitude (power)
may not be reached, especially with higher default settings (>50 %). The reason is that the HF
generator can no longer provide the required power.
• The immersion depth may not exceed 20 mm in the case of micro and tapered tips.
• When producing emulsions, the probe should be immersed to the level of the interface between
the liquids to be mixed.
• Do not use combustible solvents in open reaction vessels since the operating safety of the
homogeniser could be compromised.
• In order to shut down the device, disconnect it from the mains ( pull the plug).
17 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

4.2 General use
Before starting use, the important instructions in Chapter 4.1 should always be heeded!
Countdown operation
Action Display Remark
1
Turning on the generator
Turn "POWER" control dial all
the way to the left.
Activate the mains switch
- The display is lit after
3 seconds.
MIN/SEC: last set value
CYCLE: last set value
("-" = Max.)
POWER: 000 %
The green control light is lit
When the generator is turned on, the
most recently used operating mode
becomes active.
It is possible to switch to continuous
operation using the RESET key.
After the start-up process, the
generator will not react to further
key presses!
Numeral 5
2
Entering settings
Through progressive pressing
of the MODE key, allow the
"MIN", "SEC", and "CYCLE"
displays to flash successively.
Set the time and/or the
pulsation level with the +/-
keys. If the keys are pressed
and held for more than 2
seconds, a fast scroll will
occur!
MIN/SEC: last set value
CYCLE: last set value
("-" = Max.)
POWER: 000 %
Start is possible without entering
a new program - currently saved
settings.
No input within 10 sec. (display
flashing)
- exit from input mode,
no saving. The display resets
to the last current
value.
Numeral 5
3
Ending a programming step
(saving)
Confirm settings with the
MODE key until the display
stops flashing.
MIN/SEC: newly set value
CYCLE: newly set value
POWER: 000 %
The program entered is saved.
During operation, the POWER should
be set and is modifiable.
Numeral 5
4
Programming without
permanent saving
Do not confirm settings with
the MODE key.
MIN/SEC: newly set value
CYCLE: newly set value
POWER: 000 %
Start is possible without entering a
new program
- currently saved settings,
by pressing Start within 10 seconds.
Press the RESET key for a new
entry.
Numeral 5
5
Start
Press the START/STOP key
on the generator or transducer,
or activate the foot switch.
MIN/SEC: runs in reverse
CYCLE: newly set value
POWER: for the moment in
%
If the power display expires,
the ultrasonic transducer is
not connected. To connect the
transducer, no program should be
started. Finally, restart.
6
Interrupting
Activate the START/STOP
key on the generator, the
transducer, or the foot switch.
MIN/SEC: remaining time
CYCLE: newly set value
POWER: 000 %
During the interruption, the colon
flashes between MIN/SEC.
The user has the option of entering
new settings or to
proceed with operation.
- Currently saved setting
7
Continuation
Press the START/STOP key
on the generator or transducer,
or activate the foot switch.
MIN/SEC: Time runs in
reverse
CYCLE: newly set value
POWER: for the moment in
%
At the end of the time program,
00 min, 00 sec is displayed. The
pulsation level is retained. After a
short time, the display switches to the
previously set program.
Start
Start
Start
Start
18 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

Action Display Remark
8
Cancelling
Press the RESET key
MIN/SEC: last set value
CYCLE: newly set value
POWER: 000 %
The current program can be
cancelled at any time by pressing
the RESET key. The last set program
will be automatically displayed again.
If the RESET key is pressed once
again after the cancellation, the
generator will switch to continuous
operation.
9
Switching to continuous
operation when the
generator has stopped
Press the RESET key 1 time
MIN/SEC: 00 min 00 sec
CYCLE: newly set value
POWER: 000 %
10
Calling up a saved program
when the program has
expired
Press the RESET key once.
MIN/SEC: saved value
CYCLE: saved value
POWER: 000 %
Press once:
the saved program will appear
in continuous operation
Press twice:
the saved program will appear
in countdown operation
11
Turning off the generator
Turn the control dial all the
way to the left. Activate the
mains switch.
The display goes out.
The control light is no longer lit.
The last set program is retained.
After powering off, a pause of
approx. 10 sec must be observed
before turning the device on again.
The saved countdown operation is
retained.
Caution ! Observe the maximum allowed amplitude setting for the probes - chapter 4.3.
19 / 4451436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]

Continuous operation
Action Display Remark
1
Turning on the generator
Turn the "POWER" control dial
all the way to the left. Activate
the mains switch
- the display lights up
after 3 seconds.
MIN/SEC: last set value
CYCLE: last set value
("-" = Max.)
POWER: 000 %
The green control light is lit.
When the generator is turned on, the
most recently used operating mode
becomes active.
It is possible to switch to countdown
operation using the RESET key.
2
Start
Press the START/STOP key
on the generator or transducer,
or activate the foot switch.
MIN/SEC: runs forward
CYCLE: set value
POWER: for the moment in
%
During operation, the POWER should
be set and is modifiable. If a time of
99 min 59 sec is reached, zeros are
displayed and operation continues.
After the start-up process,
the generator will not react to
further key presses!
3
Interrupting
Press the START/STOP key
on the generator or transducer,
or activate the foot switch.
MIN/SEC: elapsed time
CYCLE: set value
POWER: 000 %
During the interruption, the colon
flashes between MIN/SEC.
The user has the option of entering
new settings or
continuing operation.
- Currently saved settings
4
Continuation
Press the START/STOP key
on the generator or transducer,
or activate the foot switch.
MIN/SEC: time continues to
run forward
CYCLE: set value
POWER: for the moment in
%
5
Cancelling
Press the RESET key
MIN/SEC: 00 min 00 sec
CYCLE: set value
POWER: 000 %
The user can restart, or switch to
countdown operation by pressing the
RESET key.
6
Switching to countdown
operation
After cancelling, press the
RESET key once again.
MIN/SEC: last saved value
CYCLE: last saved value
POWER: 000 %
Any other time than 00:00 means:
continuous operation is inactive.
In countdown operation, the setting
00:00 is not an option and will also
not be displayed.
7
Turning off the generator
Turn the control dial (POWER)
all the way to the left. Activate
the mains switch.
The display goes out.
The control light is no longer lit.
The last set program is retained.
After powering off, a pause of
approx. 10 sec must be observed
before turning the device on again.
The saved continuous operation is
retained.
Caution ! Observe the maximum allowed amplitude setting for the probes - chapter 4.3.
20 / 44 51436a GB/2017-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]