BANDELIN SONOPULS User manual

Ultrasonic homogeniser HD 2070.2
7
Ultrasonic homogenisers
User instructions
valid for:
HD 2070.2 Volumes: 1 ml - 200 ml
HD 2200.2 Volumes: 2 ml - 1000 ml
51506-002 GB/2020-10

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Copyright and disclaimer
This document may not be reproduced, whether in full or in part, without the prior approval of BANDELIN electronic GmbH & Co. KG,
hereinafter “BANDELIN”.
The German-language original is the binding version of this document. Any differences in the translation are not binding and
have no legal effect. In case of any discrepancies between the translation and the original of this document, the original version
will take precedence.
BANDELIN accepts no responsibility or liability for damage caused by improper handling or usage contrary to the intended
purpose.
The documentation has been prepared with great care. All liability is excluded for any direct or indirect losses resulting
from incomplete or erroneous information contained in this documentation, or in its presentation or usage.
All images are provided as examples and are not true to size. Decorative elements are not included in the scope of delivery.
Subject to technical changes. Measurements are subject to manufacturing tolerances.
© 2020
1 2 GmbH & Co. KG, Heinrichstrasse 3 – 4, Germany, 12207 Berlin,
Tel.: [phone] - 0, Fax: [phone], [email protected]

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General information
The equipment, the accessories and the preparations are to be used in accordance with the user
instructions and/or the product information.
The instructions are part of the scope of delivery and are to be stored in the vicinity of the device
for later reference. This also applies if possession of the device is transferred elsewhere.
Before the device is put into operation, these instructions are to be read carefully and completely
in order for the user to become familiarised with all functions.
The warnings and safety precautions (Chapter 1.5) are always to be followed during use.
The manufacturer will not assume any responsibility for the device's safety or functional ability
in the event of improper handling or usage contrary to the intended purpose. In the event of
unauthorised alterations/modifications, both the warranty claim and the CE conformity will expire.
If service is required, please contact the authorised dealer or the manufacturer.
Symbols used:
Symbol Meaning Explanation
Danger
Used to mark information that, if not observed, could
constitute a risk to life and limb, especially as a result of
electric shock.
Caution
Used to mark information that must be observed and
complied with in order to avoid damage to the device
or injury to the user.
Warning Warning of hot surface.
Important Identifies information that is important for implementation.
Note Used to mark explanatory information.
In vitro diagnostics
information
Identifies information that is important for in vitro
diagnostics applications.
Medical note Identifies information that is important for medical use.
Do not grip inside For health reasons, touching the oscillating fluid is prohibited.
Wear ear protectors For health reasons, spending long periods of time in
the vicinity of the device without ear protectors is prohibited.
Instructions Identifies instructions that must be followed
in the described sequence.

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Table of Contents
1 Product description .........................................................................................................6
1.1 Basic working principle ...................................................................................................7
1.2 Intended purpose ...........................................................................................................7
1.3 CE conformity .................................................................................................................7
1.4 Technical data ................................................................................................................ 8
1.4.1 Ultrasonic generator (GM) ..............................................................................................8
1.4.2 Technical data, ultrasonic transducers .........................................................................10
1.4.3 Remote operation ......................................................................................................... 10
1.4.4 Electromagnetic compatibility (EMC) ........................................................................... 11
1.5 Warnings and safety precautions .................................................................................12
2 Preparation ...................................................................................................................14
2.1 Scope of delivery ..........................................................................................................14
2.2 Set-up / assembly ......................................................................................................... 14
2.3 Start-up ......................................................................................................................... 15
3 Operation ......................................................................................................................15
3.1 Operating controls ........................................................................................................15
3.1.1 Turning the homogeniser on/off.................................................................................... 16
3.1.2 Explanation of the display fields ...................................................................................16
3.2 Setting the operating parameters .................................................................................16
3.2.1 Sonication time [time] ................................................................................................... 17
3.2.2 Pulsation [pulse] ........................................................................................................... 18
3.2.3 Special functions .......................................................................................................... 19
3.3 Activating / deactivating the ultrasound ........................................................................20
4 Usage ...........................................................................................................................21
4.1 Instructions for use .......................................................................................................21
4.2 General use ..................................................................................................................21
4.3 Selecting a suitable probe ............................................................................................23
4.3.1 Mounting and dismounting of the probes ..................................................................... 24
4.3.1.1 Mounting and dismounting the titanium plates .............................................................25
4.3.1.2 Mounting and dismounting of micro and tapered tips and long probes ........................26
4.4 Dismounting and mounting of the standard and booster horns .................................... 27

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5 Cleaning and maintenance of the homogeniser ...........................................................29
5.1 Cleaning and care ........................................................................................................29
5.2 Treatment of contaminated parts in the ultrasonic transducer, vessels and accessories
pertaining to the medical field .......................................................................................30
5.3 Positioning / storage .....................................................................................................30
6 Maintenance and repair ................................................................................................31
6.1 Maintenance .................................................................................................................31
6.2 Functional checks ......................................................................................................... 31
6.2.1 Testing the ultrasonic transducer (probe check) – Error 011 ........................................ 31
6.2.2 Conducting a frequency scan (scan frequency) – Error 002/011/012 .......................... 31
6.3 Troubleshooting ............................................................................................................ 33
6.4 Repairs and service ...................................................................................................... 35
6.4.1 Decontamination certificate ..........................................................................................35
7 Accessories ..................................................................................................................36
7.1 Required accessories ...................................................................................................36
7.2 Optional accessories ....................................................................................................36
8 Consumable materials ..................................................................................................37
9 Decommissioning .........................................................................................................37
10 Keyword index .............................................................................................................. 38
General information
A Tools for mounting/dismounting

810 9
4
6
4a
5
132 7
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1 Product description
The SONOPULS ultrasonic homogeniser is essentially made up of three components:
the ultrasound generator, the ultrasonic transducer, and the working tip (probe). The individual
components can be varied using a multitude of options and accessories. The type
specification and serial number are found on the type plate.
Product features:
• Ultrasonic generator (1) in easy-care robust metal housing with connection for
the ultrasonic transducer.
• Operating and display panel (2) with LCD display (3)
• Ultrasonic transducer (4) with Start/Stop button (4a)
• Standard and/or booster horn (5)
• Probe (6)
• Mains switch (7)
Product features on the rear of the ultrasonic generator:
• Panel-mounted plug (8) with fuse holder
• Ultrasonic socket (9) and/or ultrasonic transducer
• Remote control connector (10)

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1.1 Basic working principle
The ultrasonic generator transforms the mains energy input (mains frequency 50 or 60 Hz) into
high-frequency energy with a frequency of 20 kHz. Thanks to the ultrasonic transducer that is
connected to the ultrasonic generator, the high-frequency energy of the ultrasonic generator is
converted into ultrasonic and thus into mechanical energy. This takes place with an efficient and
robust PZT ultrasonic oscillating system. Thus, mechanical deflections with the same frequency
of 20 kHZ are generated on the tip of the probe and transferred into the sonication medium as
ultrasonic waves with a high power intensity. The amplitude is held constant by the ultrasonic
transducer (AMPLICHRON®system), independently from the applied load, using a signal
feedback as long as the maximum power allowed is not exceeded. Thanks to these measures,
the reproducibility of the process parameters is guaranteed and validation of the process
is supported.
Standard and/or booster horns with probes (micro tips, tapered tips or titanium plates) are
mounted on the ultrasonic transducer. These work as mechanical transformers and enable
a multiple mechanical amplification of the ultrasonic amplitude at the tip.
1.2 Intended purpose
SONOPULS ultrasonic homogenisers generate high-performance ultrasonic with high intensities
and ultrasonic amplitudes, which are transferred into liquid media using working tools called
probes. They are used in laboratories, clinics, and in industrial research, and they perform diverse
tasks during sample preparation in Quality Assurance, scientific experiments, analyses, and in
pilot or short-series manufacture.
Examples of uses:
• Cell disruption for paternity tests
• Cell disruption (extraction of micro-organisms, tissue cells)
• Homogenising of liquids
• Emulsifying hard-to-mix liquids
• Dispersion of agglomerates
• Acceleration of chemical reactions
• Degassing of fluids
• Sample preparation in environmental analytics (wastewater tests, soil samples)
• Sonochemistry
SONOPULS ultrasonic homogenisers are also used for in-vitro diagnostic lab procedures, to
procure information from the handling of organic materials with ultrasound. Therefore, they
are classified and treated in accordance with the Directive 98/79/EC on in-vitro diagnostic
medical devices.
1.3 CE conformity
SONOPULS ultrasonic homogenisers are declared as IVD products and satisfy the
CE marking criteria for the European Directives:
- IVD Directive
- Low-Voltage Directive
- Electromagnetic Compatibility Directive
- RoHS Directive
in their currently valid versions.
A declaration of conformity can be requested from the manufacturer by providing
the serial number.

Protected against access by fingers
to hazardous parts; protected against
solid foreign bodies with a diameter
of 12 mm or larger
Not protected
against ingress
of water
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1.4 Technical data
SONOPULS ultrasonic homogenisers are interference-free and marked with CE.
Safety: EN 61010-1;
EMC: EN 61326-1
1.4.1 Ultrasonic generator (GM)
Operating voltage 230 V~ (± 10 %) 50/60 Hz, alternatively 115 V~ (± 10 %)
50/60 Hz, cable length 2 m
Protection class: I
Ultrasonic frequency: 20 kHz ± 500 Hz
Frequency control1: automatic, resonance frequency search
Time settings range: 00:01 – 99:59 [mm:ss] or continuous operation
Ultrasonic operating mode: Pulsating or continuous
Pulsation time ON (tE): 1 - 60s – (see Chapter 3.2.2)
Pulsation time OFF (tA): 1 - 60s – (see Chapter 3.2.2)
Ultrasonic control: Amplitude – (see Chapter 3.2.3)
Amplitude settings range: 10 - 100 % in 1 % steps
Amplitude indicator: Presetting and progress bars
Operating controls: Rotary knob, membrane keys
Operating data display: LCD graphic display, illuminated
Remote control (turning on/off): Button on the ultrasonic transducer, potential-free contact,
foot switch TS 8 (optional)
Protection code: IP 20 pursuant to DIN EN 60529
Device-specific:
Ultrasonic generator GM 2070.2 GM 2200.2
Rated ultrasonic output2, max. 70 W 200 W
Weight 1.9 kg 2.2 kg
Exterior dimensions (L × W × H) 245 × 215 × 140 mm
1 Frequency control:
The ultrasonic generator has an automatic search feature for resonance frequency and rate adaptation while in operation. Causes for
a change in the resonance frequency are, for example, a warming of the ultrasonic transducer and the probes, a change in the acoustic
load due to changes in viscosity, and even the mounting of another probe. A frequency drift during operation is corrected by the automatic
frequency control. The optimum working frequency is sought using the resonance frequency search, e.g. after a change in probe.
2 Rated ultrasonic output:
In the case of amplitude control, the ultrasonic power that is needed for the desired probe amplitude is dependent on the viscosity of
the medium. In order to prevent damage to the ultrasonic generator and converter, the power is limited to the maximum permitted peak
value. In the case of highly-viscous media, this can also result in the desired amplitude not always being reached.

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Ambient conditions pursuant to EN 61 010-1
Overvoltage category: II
Degree of soiling: 2
Permissible relative humidity up to 31 °C 80 %
Permissible relative humidity up to 40°C: 50 %
Permissible ambient temperature: 5 to 40°C
Temperature in storage: 0 to 50 °C
Altitude: up to 2000 m
No condensation allowed.
Only for indoor operation.
Specifications for use as a medical device
Name: Ultrasonic homogeniser
UMDNS nomenclature (ECRI / DIMDI): 17-125
Purpose: See Chapter 1.2
Medical device pursuant to
Directive 98/79/EC for in-vitro diagnostic medical devices: other IVD
Type, model, serial number, year of manufacture: See type plate on the ultrasonic
generator for information
The homogeniser has been inspected pursuant to norms currently in effect and must be
installed and put into operation pursuant to EMC directions; information in this regard is
found in the Appendix.
Specifications pursuant to the German Medical Devices Operator Ordinance (MPBetreibV):
Start-up on location, functional check
and personnel training (section 5): not required
Technical safety checks, STK (section 6): no specifications
Technical measurement checks, MTK (section 11): not applicable
e-procurement
e-cl@ss:
HD device classification: 32-08-02-02
HD accessories classification: 32-08-02-05

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1.4.2 Technical data, ultrasonic transducers
UW 2070 / UW 2200
PZT ultrasonic oscillating system: 
Start/Stop key: 
Suitable for continuous operation: 
Frequency: 20 kHz
Weight: 1.2 kg
Dimensions: Ø 70 × 120 mm
Protection class: IP 20
Ambient conditions and explanation of protection class, see Chapter 1.4.
1.4.3 Remote operation
Several options are available for remote control and remote operation. Depending
on requirements, the most convenient solution may be selected.
Operating
element Methods Functions Connection
1Button manual Ultrasonic operation ON/OFF Fixed on the ultrasonic
transducer
2Foot switch foot-operated Ultrasonic operation ON/OFF Remote control sleeve
3 External control signal • Ultrasonic operation ON/OFF
• Status confirmation
Remote control sleeve
Remote control connector
Contact assignment and functional description of the remote control connector
Diagram Contact Assignment Signal Function
1
2
3
45
1 Input 0V L
5V H
L Ultrasonic operation ON
H Ultrasonic operation OFF
2 not assigned
3 Earth
4 Output 0V L
5V H
L Ultrasonic operation OFF
H Ultrasonic operation ON
5 Output As with contact 4, but with signal inverted

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Notes:
• The inputs and outputs may not be connected to other circuits, ground planes,
or ground terminals.
• The connection of power or voltage sources of any type is not permitted.
• The source resistance for the outputs is of 2.2 kΩ. The input resistance for subsequent signal
inputs, e.g. optocouplers, must be greater than 20 kΩ.
• The function of the signal input (contact 1) depends on the mode of operation that is set for
the pulsation (see Chapter 3.2.2). With the operating mode “by hand key”, control is conducted
statically, i.e. the ultrasonic operation is always activated under L status, and deactivated
under H status. With all other modes of operation, the input works as an edge-controlled
changeover switch.
• When connecting a changeover contact (toggle function) to connector 1, the make time must be
> 100 ms.
Please note:
Proper operation will depend on the length of the connection cable. If an extension is used,
a length of 3 m may not be exceeded.
1.4.4 Electromagnetic compatibility (EMC)
The device has been checked for electromagnetic compatibility in accordance with
DIN EN 61326-1 and meets the requirements for Class B devices in accordance with EN 55011.
It is suitable for use in institutions and other areas that are directly connected to a public
low-voltage power supply, e.g., medical laboratory facilities.

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1.5 Warnings and safety precautions
General information
• Keep the device and accessories out of the reach of children and also of persons who have not
been instructed in their operation by reference to these instructions.
• The use of the device or parts thereof on humans or animals is not authorised.
• Keep the ultrasonic generator and operating controls clean and dry.
• Do not expose the device to corrosive materials.
• When working with the device, please observe hygiene instructions pursuant to Chapter 5.2.
• The connection of any type of power or voltage sources to the signal inputs or outputs
is forbidden.
• Signal wires for foot switches, temperature sensors, etc., may not exceed a maximum length
of 3 m.
• All plug connections (such as for ultrasonic transducers, foot switches) may only be plugged
or unplugged while the device is turned off.
• The ultrasonic generator and ultrasonic transducer must be transported separately.
• The homogenisers adhere to prescribed EMC limit values, such that it can be assumed that the
electromagnetic radiation emanating from the units is harmless to humans. A binding statement
for wearers of implants can only be made at the place of work and together with the implant
manufacturer. In case of doubt, information regarding the allowable electromagnetic exposure
level should be obtained from the implant manufacturer.
Operation
• Observe ambient and set-up conditions, see Chapter 1.4.
• Determine the mains voltage before connecting the ultrasonic generator.
• Only connect the ultrasonic generator to an earthed socket.
• The ultrasonic transducer may only be firmly mounted on the black housing using a suitable
support, e.g. stainless steel stand.
• Before each startup, check that the probe is firmly positioned on the standard or booster horn;
if necessary, tighten the probe (see Chapter 4.3.1).
• Do not touch any oscillating parts (e.g. standard or booster horns, micro tips or tapered tips,
titanium plates, ultrasonic transducer) during operation! Damage to health is possible.
• Do not touch the sonication vessels with the oscillating probe – probes and vessels could be
damaged.
• The sonication of liquids generates noise. Suitable accessories, such as a sound proof
box, will minimise such noise considerably. If operating without a sound proof box, wear
hearing protection.
• Warning, risk of splashing!
This is especially the case with small sample quantities and when immersing oscillating probes.
• Do not use combustible solvents in open reaction vessels since the operating safety of
the homogeniser could be compromised. Safe extraction of combustible vapours must be
guaranteed. When using a sound proof box, the vapours cannot escape.
• Before any mounting or dismounting (Chapter 4.4) of probes or standard/booster horns
as well as accessories, turn offthe device and disconnect the ultrasonic transducer from
the ultrasonic generator.

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• Only use the prescribed tool for mounting and dismounting (see Appendix A).
• Do not use any bent probes (unstable operation, loss of power).
• Liquids must not penetrate the inside of the ultrasonic transducer.
• Never twist the ultrasonic transducer's black housing toward the aluminium cylinder (ultrasonic
oscillating system). The ultrasonic oscillating system and its electrical connections would be
destroyed as a result.
• Do not operate the device without supervision.
Advice for the medical and laboratory field
• The homogeniser is exclusively intended for use by skilled medical personnel.
• It may generate radio interferences or disrupt the operation of devices nearby. It may be
necessary to take remedial measures such as realigning the device or reconfiguring the
homogeniser or the shield.
• During operation, portable or mobile HF communication systems in the vicinity of the
homogeniser should be turned off– operation may be disrupted.
Damage
• If damage to the homogeniser is detected, do not connect the homogeniser to the mains.
• In the event of defects, disconnect the mains plug immediately.
• Repairs are only to be conducted by authorised skilled personnel or by the manufacturer.
• Defective parts must only be replaced with original parts or parts of the same quality!

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2 Preparation
Carefully unpack the ultrasonic generator, ultrasonic transducer and accessories, and inspect
them for completeness or possible transportation damage. If damage or a defect is found,
communicate this to the responsible forwarding agent and supplier immediately in writing.
2.1 Scope of delivery
The scope of delivery will depend on the size of the order. However, it will generally include
the following parts:
1 Ultrasonic generator (GM) ... – see delivery note, with mains cable NL 5 xx
1 Ultrasonic transducer UW ...
1 Standard horn or booster horn SH ... (already firmly screwed-in)
1 Probe
1 Tool set WZ 2
1 Manual
Additional accessories according to order – see delivery note
2.2 Set-up / assembly
Place the ultrasonic generator atop a firm, level and dry surface. In doing so,
• do not group it or stack it over other electric or non-electric devices.
• do not cover the ventilation holes on the ultrasonic transducer's housing,
• guard against moisture and wet – risk of electric shock.
During delivery, the standard or booster horn is already firmly screwed to the corresponding
ultrasonic transducer.
Other standard or booster horns are mountable. To do so, see instructions in Chapter 4.4.
Screw together the supplied probe with the standard or booster horn, see Chapter 4.3.1.
Position the ultrasonic transducer safely and correctly inside the holder.
To do so, affix the ultrasonic transducer onto the sound proof box's supporting ring or firmly
mount the ultrasonic transducer on the black housing alone, using a suitable support, e.g.
a stainless steel stand.
Connect the ultrasonic transducer to the ultrasonic generator.
To do so, insert the plug from the ultrasonic transducer into the ultrasonic connector located on
the rear side of the generator, and tighten using the threaded ring. If necessary, see Chapter 1.
Verify that the power switch is in the “0” position.
Before the mains connection, identify the pertinent mains voltage, compare it with the type
plate on the generator bottom, and only connect a suitable type to an earthed socket.
Please note:
• Avoid any contact between the contact pins on the ultrasonic transducer's plug, since the piezo
elements could generate electric loads through temperature fluctuations with a possibility of
slight electric shocks.

green light: Ultrasonic output
red light: Error messages
flashing red: Warning messages
Off: Standby mode (PAUSE)
1 LCD Display Display of the operating parameters and status information
2 “SET” key Calling up the list of editable operating parameters, moving within
the menu (forward)
3 Rotary knob Setting the operating parameters (right/left, without dead stop)
4 “START/STOP” key Starting or stopping the ultrasonic output, ending functions,
exiting the menu
5 Control LED
1
324
5
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2.3 Start-up
Check the positioning of the ultrasonic transducer in the holder.
Check the firm positioning of the probe and, if needed, clean thoroughly before first use.
Connect the ultrasonic generator to the mains (earthed socket) and switch on.
Set the probe type (see Chapter 3.1.1)
Conduct function test as described in Chapter 6.2.1.
3 Operation
3.1 Operating controls
Operation is conducted from the operating and display panel on the front side of the ultrasonic
generator:

AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
START
STOP
BANDELIN
SONOPULS HD 2070.2
probe: MS 73
o.k. [start]
MS 73
AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
Default value
Amplitude (Am) in %
Default value
Sonication time
Minutes:Seconds
(Display omitted during
continuous operation)
Default value
Pulsation time ON
(omitted with button or
continuous operation)
Default value
Pulsation time OFF
(specification omitted
with continuous operation)
Time elapsed
Minutes:Seconds
Pause
The ultrasonic generator is in “PAUSE” state –
no output is currently being emitted.
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3.1.1 Turning the homogeniser on/off
The homogeniser is turned on using the power switch on the rear side of the ultrasonic generator.
After turning on, the LCD display must light up. Initialisation occurs automatically. The ultrasonic
homogeniser will display the manufacturer's name, type designation, and the last-connected
sonotrode [probe].
If the probe type displayed does not conform with the probe that is mounted, the correct probe
type will have to be set before the next step using the rotary knob. By pressing the “START/STOP”
key, the menu switches into stand-by mode (PAUSE) and the ultrasonic homogeniser is ready for
operation. The parameters used during the last operation will be set again and displayed.
The mains switch can also be used for powering off.
When the ultrasonic generator is powered off, the memory contents are saved and become
available again when the generator is powered on.
3.1.2 Explanation of the display fields
3.2 Setting the operating parameters
With the exception of the amplitude, the operating parameters can only be set while in stand-
by mode (PAUSE). The editing mode is activated by pressing the “SET” button. It is possible to
switch between the individual parameters by repeatedly pressing the “SET” button. In the marked
area (inverse view) of the selected parameter, the desired value is set by turning the rotary knob.
By pressing the “START/STOP” button, the editing mode is exited.

AM 25% PAUSE
time non-stop
pulse on 01s off 01s
AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
time 01:30 00:00 time non-stop
AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
time 01:30 00:00 SET
SET
AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
1 to 59 seconds1 to 99 minutes
01 01:30
SET
START
STOP
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The following operating parameters can be set:
• Default values for the amplitude [AM]
• Default values for the sonication time [time]
• Default values for the pulsation of the ultrasound (turn-on and turn-offtime) [pulse]
3.2.1 Sonication time [time]
A default value of 99 minutes and 59 seconds (99:59) is the maximum sonication time that can
be set. If the default value is exceeded during ultrasonic operation, the sonication will stop.
The parameter “non-stop” is equivalent to unlimited continuous operation until ultrasonic operation
is ended by pressing the “START/STOP” key.
a) Set continuous operation [non-stop]
b) Setting the sonication time
If a value of 59 seconds is exceeded when setting the time, or if a shortfall occurs when setting
back the time, the values for the set minutes will change accordingly.
proceed to next set value or operating parameter
back to stand-by mode

AM 25% PAUSE
time 01:30 00:00
pulse 000.5s 0001.0s
AM 25% PAUSE
time 01:30 00:00
pulse 01s 01s
pulse on 01s off 01s pulse off
AM 25% PAUSE
time 01:30 00:00
pulse 000.5s 0001.0s
pulse by hand key
AM 25% PAUSE
time 01:30 00:00
pulse 000.5s 0001.0s
SET
pulse 01s 01s
START
STOP
AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
AM 25% PAUSE
time 01:30 00:00
pulse on 01s off 01s
1 to 60 seconds
SET SET
1 to 60 seconds
01 01
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3.2.2 Pulsation [pulse]3
In addition to setting the pulse turn-on time tEand the pulse turn-offtime tA, two additional
operating modes can also be set:
offNo pulsation or continuous sound
by hand key It is possible to pulse manually with the key on the ultrasonic transducer.
Ultrasonic operation will be active as long as the key on the ultrasonic
transducer is pressed.
a) Setting the operating modes
b) Setting the time intervals
The maximum setting value is 60.0 s. The minimum setting value at 1 s.
Turning-on time tETurning-offtime tA
back to stand-by mode
3 The pulse period duration is the sum of the turn-on and turn-offtime t = tE+ tA

probe check off
scan frequency off
contrast 197
V xxxx 199547 Hz
off
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3.2.3 Special functions
Additional functions are available within a second menu level. These can be accessed by pressing
and holding the “SET” button for approximately 2 seconds. Switching between these functions is
accomplished in the manner already described. The menu level can only be exited by pressing the
“START/STOP” key. In doing so, settings and function statuses are carried over and executed.
Check the ultrasonic transducer
Search resonance frequency
Set monitor contrast
Software version and latest start frequency
Check the ultrasonic transducer [probe check]
This function is used for quick verification of whether the ultrasonic transducer is recognised by
and can be correctly activated by the ultrasonic generator. After a change in probe or in operating
conditions it is possible, for example, that the ultrasonic generator cannot synchronise with the
ultrasonic transducer and issues an error message, e.g. Error 011. The ultrasonic frequency is
reset to the base value and the function is restored if no device fault or other cause is present.
The default value is “off”. In order to activate the function, set the parameter to “start”
using the rotary knob, and then press the “START/STOP” button. See Chapter 6.2.1 for
additional information.
Search for resonance frequency [scan frequency]
This function is an expansion of the above-mentioned “probe check” function. In addition, a
frequency scan is conducted in order to determine the correct HF or ultrasonic frequency for the
ultrasonic transducer. Depending upon the probe used and the operating conditions, the frequency
can fluctuate significantly, to some extent. When switched off, the ultrasonic generator saves
the current frequency value and uses it as the start value when switched on again. If there are
significant deviations in the current ultrasonic frequency, this can lead to malfunctions.
Please note:
After a successful scan while in continuous operation (pulsation = off, time > 30 s), start with
an amplitude setting of 50 % and check the operation. Next, set the desired default value.
Setting the monitor contrast [contrast]
With this function, the monitor contrast can be adapted to the local light conditions. The contrast
values range between 1 and 255, and can be set by turning the rotary knob. Smaller values result
in a darker image and larger values result in a lighter image. The default value is 240.

Am 35%
time 01:30 00:30
pulse on 01s off 02s
Elapsed
sonication time
Actual valueSet value
20 / 42 51506-002 GB/2020-10
1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]
3.3 Activating / deactivating the ultrasound
Ultrasonic operation can be started and stopped by pressing the “START/STOP” key or the key on
the ultrasonic transducer. Ultrasonic operation ends once the target time has elapsed (if a time has
been set), and a signal tone (long + short) is emitted. When the device is restarted, the displays
for the elapsed sonication time and for the energy are set to zero.
In contrast to stand-by mode, ultrasonic operation displays the actually achieved value in bar
graph form, in addition to the pre-set amplitude.
Please note:
Depending upon the physical conditions, i.e. the viscosity of the sonicated medium and the thus-
resulting control processes, a difference between the target and the actual value, as well as a
fluctuating display, may occur.
The amplitude can be changed at any time during ultrasonic operation, using the rotary knob.
All other operating parameters can only be edited while in stand-by mode (see Chapter 3.2).
Notes:
• If the ultrasonic operation is manually interrupted before it reaches the activated target time, the
display values for the elapsed sonication time and for the energy will be saved and will continue
running upon restart. The display values can be reset to zero by pressing the “SET” key and
then pressing the “START/STOP” key.
• With the “non-stop” setting, the elapsed sonication time and energy must also be manually
reset, if needed. Once the maximum displayable time has been reached and exceeded, the
time display will start again at 00:00.