BANDELIN SONOPULS HD 5020 User manual

51846-000 en/2022-07
Instructions for use
SONOPULS
Ultrasonic homogeniser
Valid for:
SONOPULS HD 5020
SONOPULS HD 5050
SONOPULS HD 5100
SONOPULS HD 5200
SONOPULS HD 5400
Dokument:51846-000Sprache: enVersion:2022-07

2/36 51846-000 en/2022-07
© 2022
1 2 GmbH & Co. KG, Heinrichstr. 3–4, 12207 Berlin, Ger-
many
Phone: [phone], Fax: [phone], [email protected]
Certified in accordance with ISO 9001 and ISO 13485

51846-000 en/2022-07 3/36
Table of contents
Table of contents
1 About this operating manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1 Use of the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Explosion hazard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.3 Hazards due to high temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.4 Danger of electric shock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5 Danger due to ultrasound. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.6 Danger due to the sonicating medium . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.7 Harmful to health due to ultrasound noise . . . . . . . . . . . . . . . . . . . . . 8
2.8 Keep out of reach of children . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.9 Damage to the probe and sonication vessel . . . . . . . . . . . . . . . . . . . . 8
2.10 Disturbance of wireless communication . . . . . . . . . . . . . . . . . . . . . . . 8
3 Design and function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.2 Symbols and buttons on the touch screen . . . . . . . . . . . . . . . . . . . . . 10
3.3 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4 Preparation for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.1 Set up homogeniser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.2 Mounting/removing the probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.3 Mounting/removing standard horn or booster horn. . . . . . . . . . . . . 12
4.4 Switching the device on and off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.5 Select probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.1 Immersion depth notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2 Preselect parameters for sonication . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2.1 Set amplitude or power. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2.2 Enter sonication time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.2.3 Set pulsation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.2.4 Setting temperature monitoring. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.3 Start sonication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.4 Creating and starting programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.4.1 About programs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Table of contents
4/36 51846-000 en/2022-07
5.4.2 Creating a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.4.3 Activating and deactivating a program . . . . . . . . . . . . . . . . . . . . . . . . 18
5.4.4 Starting a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.5 Creating documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.6 Set settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.6.1 Adjusting the button function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.6.2 Adjust brightness. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.6.3 Set the language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.6.4 Setting the time and date . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.6.5 Set sleep mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.6 Configuring the network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6.7 Set temperature unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.7 Troubleshooting a malfunction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.1 Cleaning the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.2 Refurbish probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.3 Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
8 Device information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8.1 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
8.3 CE conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
9 Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.1 Available probes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
9.2 Optional accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
9.3 Optional tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

51846-000 en/2022-07 5/36
About this operating manual/1
1 About this operating manual
These instructions for use contain necessary and useful information on how to
operate the ultrasound bath safely and efficiently.
• Read these instructions for use before using the ultrasound bath.
• Pay special attention to chapter 2Safety.
• If you pass this ultrasound bath on to others, please enclose these instruc-
tions for use.
• Contact your specialist dealer or BANDELIN if any of your questions are not
answered in these instructions for use. Information on service can be found in
chapter 6.3Repair.
Illustrations are provided as examples and are not to scale.

2 / Safety
6/36 51846-000 en/2022-07
2Safety
2.1 Use of the device
The device is used in laboratories, clinics and in industrial research. It performs
diverse tasks during sample preparation in Quality Assurance, scientific experi-
ments, analyses, and in pilot or short-series manufacture.
Examples of applications are:
• Disruption of cells, bacteria, viruses, tissue
• Production of the finest emulsions
• Homogenising of substances
• Degassing of liquids
• Sample preparation for the grain size analysis
• Acceleration of chemical reactions
• Sample preparation for sewerage analysis
2.2 Explosion hazard
Do not use the device with combustible liquids as a sonicating medium.
Combustible vapours may be generated when flammable liquids are sonicated.
Vapours may ignite, e.g. on electrical components, resulting in an explosion.
Do not use combustible solvents in open sonication vessels, as the operational
safety of the device could be compromised. Safe extraction of combustible
vapours must be guaranteed.
2.3 Hazards due to high temperature
Ultrasound heats up the sonicating medium. The device, the sonicating medium
and the sonication vessel can become very hot during prolonged ultrasound oper-
ation. Contact with these products may cause burns.
• Do not leave the ultrasound switched on for longer than necessary.
• Allow the probe and sonication vessel to cool down before touching them.
Non-aqueous liquids can heat up many times faster than water. A possible flash-
point can be reached or exceeded after a very short sonication time. In the case of
high-boiling liquids, the temperature can rise to over 120 °C due to the energy
input of the ultrasound. This can lead to fires and severe burns.

51846-000 en/2022-07 7/36
Safety/2
2.4 Danger of electric shock
The device is an electrical device. Failure to follow safety rules can result in a life-
threatening electric shock.
• Protect the device from moisture and wetness. Keep the surfaces and the
touchscreen clean and dry.
• Do not spray or expose the device to splash water.
• Do not cover the device.
• Position the device so that it is easy to unplug the mains connection at any
time and without difficulty.
• Only connect the device to a power outlet with a grounded socket.
• If you notice a defect in the device, disconnect the mains plug immediately. Do
not connect a defective device to the mains.
• Repairs should only be carried out by the manufacturer.
2.5 Danger due to ultrasound
Strong ultrasound destroys cell structures. Exposing a body part to ultrasound
can lead not just to skin damage, but also to internal tissue damage. On fingers,
the periosteum can become damaged.
• Do not touch the probe, the booster horn or the standard horn during opera-
tion. Do not touch the sonication fluid during operation.
• Never sonicate living beings.
2.6 Danger due to the sonicating medium
The sonicating media treated with the device can be toxic, corrosive or otherwise
harmful to health. Vapours and aerosols can also be dangerous.
• Wear gloves and safety goggles when handling hazardous preparations.
• Make sure that the sonicating medium does not splash. This can particularly
occur with small quantities of sonicating medium. Only switch on the device
once the probe has been immersed in the sonicating medium. Start sonication
with a low amplitude.
• Do not lean over the ultrasound bath, in order to avoid vapours from coming
into contact with the eyes or from being inhaled.
• In case of dangerous vapours, use a suction device.

2 / Safety
8/36 51846-000 en/2022-07
2.7 Harmful to health due to ultrasound noise
The ultrasound noise typical of the process can be perceived as very unpleasant.
Remaining within a radius of 2 m for an extended period of time may cause dam-
age to health.
• Use a sound proof box; see 9Accessories. The sound proof box significantly
reduces the noise level.
• When operating without a sound proof box, wear hearing protection.
2.8 Keep out of reach of children
Children cannot identify hazards posed by the ultrasound bath. Therefore, keep
the ultrasound bath out of the reach of children.
2.9 Damage to the probe and sonication vessel
Probes are sensitive to shock. Handle the probes with care.
Make sure that the probe does not touch the sonication vessel. This can damage
the probe and the vessel.
Foreign bodies on the contact surface between the probe and standard horn or
booster horn can destroy both parts. Therefore, always clean the contact surfaces
thoroughly before mounting the probe.
The sound-emitting surface of the probe is subject to erosion. Erosion reduces
the efficiency of the sonication process. The sonication of corrosive liquids leads
to increased wear.
The sound-emitting surface can be reconditioned up to a certain point of erosion.
See 6.2Refurbish probe.
2.10 Disturbance of wireless communication
The device may interfere with other wireless communication ultrasound baths in
the immediate vicinity.
If interference occurs with the operation of a wireless device, move it further
away from the ultrasound bath.

51846-000 en/2022-07 9/36
Design and function/3
3 Design and function
3.1 Overview
Fig. 1 Overview of the device
1Ultrasoundgenerator
2 Touchscreen
3 Connector for ultrasonic converter
4LANconnection
5USBTypeBport
6USBTypeAport
7Mainsswitch
8 Cold device socket with fuse holder
9 Ultrasonic converter
10 Start/Stop key
11 Standard horn/booster horn
12 Probe
21
5
6
7
8 10
11
12
9
4
3

3 / Design and function
10/36 51846-000 en/2022-07
3.2 Symbols and buttons on the touch screen
3.3 Function
The device transforms the absorbed grid energy into high-frequency energy. The
ultrasonic converter converts the high-frequency energy into ultrasound. At the
tip of the probe, mechanical deflections are created that are transferred into the
sonicating medium as ultrasonic waves with a high power density. The standard
horn or booster horn allows multiple amplification of the mechanical amplitude at
the probe tip.
For sonication, the power or amplitude can be selected as the reference variable.
If the amplitude is selected as the reference variable, it is kept constant by the
ultrasonic converter regardless of the load, provided that the permissible maxi-
mum power is not exceeded. An optional temperature sensor enables the tem-
perature of the sonicating medium to be monitored.
The device saves documentation of its function. Documentation can be backed up
via a USB port or LAN port for evaluation and archiving.
The device is operated with a touchscreen. In addition, the ultrasound can be
switched on and off with a button on the ultrasonic converter.
Return to previous screen
Reset values
Confirm/Save
Go to startup screen
Calling up the screen help
Start
Pause
Stop