Leica CM1520 User manual

Leica CM1520
Cryostat
Instructions for Use
English
Order No.: 14 0491 81101 - Revision K
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.0, Revision K - 10.2019

3
Leica CM1520
The information, numerical data, notes and value judgments contained in this Instructions for Use
represent the current state of scientic knowledge and state-of-the-art technology as we understand it
following thorough investigation in this eld.
We are under no obligation to update the present Instructions for Use periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this Instructions for Use.
To the extent permitted in accordance with the national legal system as applicable in each individual
case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc. contained
in this Instructions for Use. In particular, no liability whatsoever is accepted for any nancial loss or other
consequential damage caused by or related to compliance with statements or other information in this
Instructions for Use.
Statements, drawings, illustrations and other information regarding the contents or technical details of
the present Instructions for Use are not to be considered warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical specications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve our technology and manufacturing
techniques used in our products.
This document is protected under copyright laws. All copyrights to this documentation are held by Leica
Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (also of any parts thereof) by means of print, photocopy,
microche, web cam or other methods – including all electronic systems and media – requires express
prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the nameplate on the back of
the instrument.
Note
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
69226 Nussloch
Germany
Tel.: +49 - (0) 6224 - 143 0
Fax: +49 - (0) 6224 - 143 268
Web: www.LeicaBiosystems.com

4Version 2.0, Revision K
Table of Contents
1. Important Information ..................................................................................................6
1.1 Symbols used in the text and their meaning................................................................................. 6
1.2 Qualicationofpersonnel .......................................................................................................... 9
1.3 Intended use of the instrument ................................................................................................... 9
1.4 Instrument type ....................................................................................................................... 10
2. Safety......................................................................................................................11
2.1 Safety Notes............................................................................................................................ 11
2.2 Hazard warnings...................................................................................................................... 12
2.3 Safety devices ......................................................................................................................... 12
2.3.1 Locking/unlocking the handwheel............................................................................................. 12
2.3.2 Safety guard ............................................................................................................................ 13
2.4 Operating Conditions ............................................................................................................... 13
2.5 Operating the instrument.......................................................................................................... 14
2.6 Cleaning and disinfection......................................................................................................... 15
2.7 Removing the microtome.......................................................................................................... 15
2.8 Maintenance............................................................................................................................ 16
3. Technical Data ..........................................................................................................17
4. Installing the Instrument.............................................................................................20
4.1 Installation site requirements ................................................................................................... 20
4.2 Unpacking instructions for the Leica CM1520 cryostat .............................................................. 21
4.3 Standard delivery - packing list ................................................................................................. 24
4.4 Installing the handwheel........................................................................................................... 25
5. Instrument Setup.......................................................................................................26
5.1 Electrical connection................................................................................................................ 26
5.2 Preparations for starting up the instrument ............................................................................... 26
5.3 General overview of the Leica CM1520...................................................................................... 28
5.4 Power switch and circuit breaker .............................................................................................. 30
5.5 Switching on the instrument ..................................................................................................... 30
6. Operating the Instrument............................................................................................31
6.1 Control panel 1 ........................................................................................................................ 31
6.2 Conguringthedesiredvalues.................................................................................................. 31
6.2.1 Setting the time ....................................................................................................................... 31
6.2.2 Setting the automatic defrost time (cryochamber)..................................................................... 32
6.2.3 Programming the temperature of the cryochamber .................................................................... 32
6.2.4 Manual defrosting of the quick freeze shelf............................................................................... 33
6.2.5 Manual defrosting of the cryochamber...................................................................................... 33
6.2.6 Setting the section thickness.................................................................................................... 34
6.3 Display lock............................................................................................................................. 34
6.4 Control panel 2 – Electric coarse feed....................................................................................... 35
7. Daily Use of the Instrument .........................................................................................36
7.1 The sectioning process ............................................................................................................ 36
7.2 Specimen freezing ................................................................................................................... 36
7.2.1 Quick-freeze shelf.................................................................................................................... 37

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Leica CM1520
Table of Contents
7.3 Specimen discs ....................................................................................................................... 38
7.3.1 Inserting the specimen discs into the specimen head ................................................................ 38
7.3.2 Specimen orientation ............................................................................................................... 38
7.4 Sectioning ............................................................................................................................... 39
7.4.1 Inserting the blade/knife holder base........................................................................................ 39
7.4.2 Clearance angle adjustment ..................................................................................................... 40
7.4.3 Premium blade holder .............................................................................................................. 41
7.4.4 Blade holder CE ....................................................................................................................... 42
7.4.5 Blade holder CE-TC .................................................................................................................. 47
7.4.6 Knife holder CN........................................................................................................................ 48
7.4.7 Cleaning the blade and knife holders......................................................................................... 53
7.5 Temperature selection chart (in minus °C)................................................................................. 56
7.6 Defrosting ............................................................................................................................... 57
7.6.1 Automatic defrosting of the cryochamber ................................................................................. 57
7.6.2 Manual defrosting of the cryochamber...................................................................................... 57
7.6.3 Manual defrosting of the quick freeze shelf............................................................................... 58
7.7 Ending work............................................................................................................................. 58
7.7.1 Finishing the daily routine......................................................................................................... 58
7.7.2 Switching off the instrument for a longer period ........................................................................ 59
8. Troubleshooting........................................................................................................60
8.1 Error messages in the display................................................................................................... 60
8.2 Temperature control button ...................................................................................................... 61
8.3 Possible sources of error, causes and remedies ........................................................................ 62
8.3.1 Changing the battery................................................................................................................ 65
9. Cleaning, Disinfection, Maintenance.............................................................................66
9.1 Cleaning.................................................................................................................................. 66
9.2 Disinfection at room temperature.............................................................................................. 66
9.3 Maintenance............................................................................................................................ 67
9.3.1 General maintenance instructions............................................................................................. 67
9.3.2 Replacing the fuses ................................................................................................................. 69
9.3.3 Removing the sliding window ................................................................................................... 70
9.3.4 Replacing the LED illumination ................................................................................................. 70
10. Ordering Information, Components and Consumables......................................................71
10.1 Ordering information................................................................................................................ 71
10.2 Mobile heat extractor - application............................................................................................ 94
10.3 Cold extraction - using the Thermal block.................................................................................. 94
10.4 Stationary heat extractor (optional) - application ....................................................................... 95
10.5 Installing the shelf (movable) (optional).................................................................................... 96
11. Warranty and Service .................................................................................................97
12. DecontaminationCerticate........................................................................................98

6Version 2.0, Revision K
Important Information
1
1. Important Information
1.1 Symbols used in the text and their meaning
Warning
Leica Biosystems GmbH assumes no liability for consequential loss or damage due to failure to
observe the following instructions, particularly in relation to transportation and package handling, and
failure to observe the instructions for handling the instrument carefully.
Symbol: Title of the symbol: Hazard warning
Description: Warningsaredisplayedinawhiteeldwithanorange
title bar and are marked by a warning triangle.
Symbol: Title of the symbol: Note
Description: Notes, i.e. important information for the user, are
displayedinawhiteeldwithabluetitlebarandare
markedbyanoticationsymbol.
Symbol: Title of the symbol: Item number
→"Fig. 7 - 1"Description: Item numbers for numbering illustrations.
Numbers in red refer to item numbers in illustrations.
Symbol: Title of the symbol: Buttons and switches on the instrument
Power switch Description: Buttons and switches on the instrument that are
supposed to be pressed by the user in various
situations are displayed as bold, gray text.
Symbol: Title of the symbol: Function key
Save Description: Software symbols that have to be pressed on the
display or information that is output on the display are
displayed as bold, gray text.
Symbol: Title of the symbol: Notice
Description: Indicates the need for the user to consult the
Instructions for Use for important cautionary
information such as warnings and precautions that
cannot, for a variety of reasons, be presented on the
medical device itself.
Symbol: Title of the symbol: Manufacturer
Description: Indicates the manufacturer of the medical device.
Symbol: Title of the symbol: Date of Manufacture
Description: Indicates the date when the medical device was
manufactured.

7
Leica CM1520
Important Information 1
Symbol: Title of the symbol: CE Compliance
Description: The CE marking is the manufacturer's declaration that
the medical device meets the requirements of the
applicable EC directives.
Symbol: Title of the symbol: In vitro diagnostic medical device
Description: Indicates a medical device that is intended to be used
as an in vitro diagnostic medical device.
Symbol: Title of the symbol: China RoHS
Description: Environmental protection symbol of the China RoHS
directive. The number in the symbol indicates the
"Environment-friendly Use Period" of the product in
years. The symbol is used if a substance restricted
in China is used in excess of the maximum permitted
limit.
Symbol: Title of the symbol CSA test symbol (USA/Canada)
Description: The CSA test symbol means that a product has
beentestedandfulllstheapplicablesafetyand/
or performance standards, including the relevant
standardsdenedoradministeredbytheAmerican
National Standards Institute (ANSI), Underwriters
Laboratories (UL), the Canadian Standards
Association (CSA), the National Sanitation Foundation
International (NSF) and others.
Symbol: Title of the symbol: WEEE symbol
Description: The WEEE symbol, indicating the separated
collection of electrical and electronic equipment
wastes, consists of the crossed-out wheeled bin
(§ 7 ElektroG).
Symbol: Title of the symbol: Alternating current
Symbol: Title of the symbol: Article number
Description: Indicates the manufacturer's catalog number so that
themedicaldevicecanbeidentied.
Symbol: Title of the symbol: Serial number
Description: Indicates the manufacturer's serial number so that a
specicmedicaldevicecanbeidentied.
Symbol: Title of the symbol: Consult Instructions for Use
Description: Indicates the need for the user to consult the
Instructions for Use.

8Version 2.0, Revision K
Important Information
1
Symbol: Title of the symbol: Flammable liquid
Description: Package labeling in accordance with German
Hazardous Freight Ordinance Road and Rail
(GGVSE)/European Agreement concerning the
International Carriage of Dangerous Goods by Road
(ADR) for transporting hazardous goods.
Class 3: FLAMMABLE LIQUID.
Symbol: Title of the symbol: Flammable freezing sprays prohibited
Description: Thissymbolalertstheuserthattheuseofammable
freezing sprays in the cryostat chamber is prohibited
due to explosion hazard.
Symbol: Title of the symbol: IPPC symbol
Description: The IPPC symbol includes:
IPPC symbol
• Country code in accordance with ISO 3166,
e.g. DE for Germany
• Regionalidentier,e.g.HEforHessen
• Producer/treatment provider code, uniquely
assigned number starting with 49
• Treatment method, e.g. HT (heat treatment)
Symbol: Title of the symbol: Stack limit
Description: Stacking of the transport packages is not allowed and
no load should be placed on the transport packages.
Symbol: Title of the symbol: Fragile, handle with care
Description: Indicates a medical device that can be broken or
damaged if not handled carefully.
Symbol: Title of the symbol: Store dry
Description: Indicates a medical device that needs to be protected
from moisture.
Symbol: Title of the symbol: Upper
Description: Indicates the correct upright position of the package.

9
Leica CM1520
Important Information 1
Symbol: Title of the symbol: Temperature limit for transport
Description: Indicates the temperature limits for transport to which
the medical device can be safely exposed.
Symbol: Title of the symbol: Temperature limit for storage
Description: Indicates the temperature limits for storage to which
the medical device can be safely exposed.
Appearance: Indication: Tilt indicator
Description: Indicator to monitor whether the shipment has been
transported and stored in upright position according
to your requirements. With a pitch of 60° or more,
thebluequartzsandowsintothearrow-shaped
indicator window and sticks there permanently.
Improper handling of the shipment is immediately
detectableandcanbeprovendenitively.
1.2 Qualicationofpersonnel
The Leica CM1520 may only be operated by trained laboratory personnel. Prior to operating the
instrument, the operator must carefully read and understand these Instructions for Use and must
familiarize him/herself with all technical details of the instrument. The instrument is intended for
professional use only.
1.3 Intended use of the instrument
TheLeicaCM1520isahigh-performancecryostatforroutineandresearchapplicationsintheeldsof
biology, medicine and industry. The instrument is used to freeze and section specimen material quickly.
The instrument is not designed for the storage of specimen material. The instrument may only be
operated within the scope of its designated use as described above and as per the instructions given in
these Instructions for Use. The Leica CM1520 is suitable for in-vitro diagnostics (IVD).
Any other use of the instrument is considered improper.

10 Version 2.0, Revision K
Important Information
1
1.4 Instrument type
All information contained in these Instructions for Use applies exclusively to the instrument type
indicated on the title page. A nameplate bearing the instrument serial number (SN) is attached to the
back of the instrument.
These Instructions for Use include important instructions and information related to the operating safety
and maintenance of the instrument. The Instructions for Use are an important part of the product, and
must be read carefully prior to startup and use and must always be kept near the instrument.
If additional requirements on accident prevention and environmental protection apply in the country
of operation, these Instructions for Use must be supplemented by appropriate instructions to ensure
compliance with such requirements.
Proper and intended use includes the observance of all instructions in the Instructions for Use and
compliance with all inspection and maintenance instructions.

11
Leica CM1520
Safety 2
2. Safety
2.1 Safety Notes
Warning
The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes
even if you are already familiar with the operation and use of other Leica products.
These Instructions for Use include important instructions and information related to the operating safety
and maintenance of the instrument.
The Instructions for Use are an important part of the product, must be read carefully prior to startup and
use and must always be kept near the instrument.
This instrument has been built and tested in accordance with the safety requirements for electrical
equipment for measurement, control and laboratory use.
Residual risks
The instrument has been designed and constructed with the latest state-of-the-art technology and
according to recognized standards and regulations with regard to safety technology. Operating or
handling the instrument incorrectly can place the user or other personnel at risk of injury or can cause
damage to the instrument or other property. The machine may be used only as intended and only if all
of its safety features are in proper working condition. Malfunctions that impair safety must be remedied
immediately.
To maintain this condition and ensure safe operation, the user must observe all notes and warnings
contained in these Instructions for Use.
Only original spare parts and permitted original accessories may be used.
Note
These Instructions for Use must be appropriately supplemented as required by the existing regulations
on accident prevention and environmental safety in the operator's country.
The instrument's EC Declaration of Conformity can be found on the Internet at:
http://www.LeicaBiosystems.com
Warning
• Theprotectivedevicesonbothinstrumentandcomponentsmustneitherberemovednormodied.
OnlyservicepersonnelqualiedbyLeicamayrepairtheinstrumentandaccesstheinstrument's
internal components.
• Duetoexplosionhazard,theuseofammablefreezingspraysinsidethecryostatchamber,while
the instrument is turned on, is prohibited.

12 Version 2.0, Revision K
Safety
2
2.2 Hazard warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for accident
prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as the
designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following instructions
and warnings.
2.3 Safety devices
The instrument is equipped with the following safety devices: Handwheel lock and safety guard on the
knife holder.
The consistent use of these safety features and strict observation of the warnings and cautions in these
Instructions for Use will safeguard the operator by and large from accidents and/or personal injury.
Personal safety precautions
Warning
When working with cryostats, personal safety precautions must always be taken. The wearing of work
safety shoes, safety gloves, laboratory coat, mask and safety goggles is mandatory.
2.3.1 Locking/unlocking the handwheel
Warning
Rotate the handwheel only if the cryostat is cooled and the cryochamber is cold.
Warning
Always lock the handwheel prior to manipulating the knife and specimen, changing the specimen, or
taking a break.
To lock the handwheel, move its handle to the 12 o'clock (→"Fig. 1-1") or 6 o'clock (→"Fig. 1-2")
position. Press the lever (→"Fig. 2-1") fully outward; gently rock the handwheel back and forth until the
locking mechanism clicks into place noticeably.
To release the handwheel, press the lever (→"Fig. 2-2") on the handwheel towards the cryostat housing.

13
Leica CM1520
Safety 2
Fig. 1
2
1
Fig. 2
1
2
2.3.2 Safety guard
Warning
Prior to manipulating the knife and specimen, changing the specimen or taking a break, always lock the
handwheel and cover the cutting edge with the safety guard.
The CN knife holder and the premium blade holder feature safety guards; the glass anti-roll plate of the
CE and CE-TC blade holders also serves as a safety guard.
2.4 Operating Conditions
Transport and installation
• After transporting, do not turn the instrument on for a minimum of 4 hours!
• A waiting period is necessary to allow the compressor oil, which may have been displaced during
transport, to return to its original position. Failure to comply with this may cause damage to the
instrument.
• The instrument may not be operated in hazardous locations!
• To ensure proper functioning of the instrument, it must be set up while maintaining a minimum
distance on all sides from walls and furniture:
- rear: 15 cm
- right: 30 cm
- left: 15 cm.

14 Version 2.0, Revision K
Safety
2
Electrical connection
• Before connecting the instrument to an AC power outlet, ensure that the AC power of your laboratory
matches the values on the nameplate of the instrument!
• The minimum rated voltage must be maintained during the startup phase of the cooling system - (→
P.17–3.TechnicalData)! The compressor draws a starting current between 45 and 50 A. Therefore,
the electric circuit at the installation site must be inspected by an electrician to ensure that it meets
the requirements for a smooth operation of the instrument. A stable power supply in accordance with
theinstrument'sspecicationsisessentialtoitsproperfunctioning.Failuretocomplywiththeabove
can damage the instrument.
• No additional extension cord may be used.
Defrosting
Warning
The quick freeze shelf can become very hot during the defrosting process! Therefore, do not touch it!
2.5 Operating the instrument
• Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp
and can cause serious injuries!
Warning
We strongly recommend using the safety gloves included in (→P.24–4.3Standarddelivery-
packing list)!
• Before leaving the instrument or taking it out of operation, always remove the knife/blade from the
knife/blade holder and do not leave open knives/blades lying around after removal!
• Never place a knife on a table with the cutting edge facing upward!
• Never try to catch a falling knife/blade!
• Alwaysclampthespecimenrst,thentheknife/blade!
• Before handling the specimen or the knife/blade, changing the specimen or taking a break, lock the
handwheel and ensure that the knife is covered by the safety guard!
• Extended skin contact with the cold parts of the instrument may cause freezer burns!
• Toensureasecureowofcondensateandtopreventapotentialcontaminationrisk,thedraintube
located on the side next to the instrument (→"Fig. 54-1") must be inserted into a suitable waste
container!

15
Leica CM1520
Safety 2
2.6 Cleaning and disinfection
We strongly recommend defrosting the Leica CM1520 cryostat completely for approx. 24 hours, 1-2x
annually or as needed.
• It is not necessary to remove the microtome for routine disinfection.
Note
Remove section waste after EVERY change of specimen! Do not start disinfection until you have done
this! Each new specimen is a potential source of contamination. When disinfecting the instrument, take
appropriate protective measures (gloves, mask, protective clothing, etc.).
Warning
• Use only alcohol-based disinfectants for disinfecting the cryochamber.
• Do not use any solvents (xylene, acetone, etc.) for cleaning or disinfection.
• Prior to disinfection with alcohol-based disinfectants, switch off the instrument and unplug the
power plug.
• Whenusingdetergentsanddisinfectants,observethespecicationsoftherespectivemanufacturer.
• Risk of explosion when using alcohol: Provide proper ventilation and ensure the instrument is
switched off.
• Risk of icing – Disinfected accessories must be fully dry before reinserting them in the chamber. Do
not switch the instrument back on before the cryochamber is fully dry.
• Dispose of waste liquids from disinfection/cleaning as well as of section waste according to
applicable regulations on disposal of special category waste!
• Refer to (→P.66–9.2Disinfectionatroomtemperature) for information on disinfection at room
temperature.
Aftereachdisinfection,thechambermustbewipedcarefullywithaclothandbrieyventilated!Before
switching on the instrument again, proper ventilation of the chamber shall be ensured.
Note
Please contact Leica Biosystems for further details on adequate disinfection measures.
2.7 Removing the microtome
• The microtome is encapsulated and therefore does not require removal by the user.

16 Version 2.0, Revision K
Safety
2
2.8 Maintenance
Replacing the fuses
• Turn the instrument off and disconnect the power plug before replacing the fuses.
• Useonlythefusetypesspeciedin(→P.17–3.TechnicalData). Failure to comply with this can
cause severe damage to the instrument and its surroundings!

17
Leica CM1520
Technical Data 3
3. Technical Data
Note
Operatingtemperaturerange(ambienttemperature):18 °Cto35 °C.Allspecicationsrelatedto
temperatureofthecoolingunitarevalidonlyforanambienttemperatureof22 °Candarelative
humidityofnomorethan60 %.
Equipmentidentication
Instrument type Model numbers
CM1520 14049148255 14049148055 14049148053,
14049148056
14049148054
Electricalspeciations
Nominal voltage (+10 %) 100 VAC 120 VAC 230 VAC 240 VAC
Nominal frequency 50/60 Hz 60 Hz 50/60 Hz 50/60 Hz
Power consumption 1440 VA 1440 VA 1440 VA 1440 VA
Power supply IEC 60320-1 C-20
Max. start-up current for 5 sec. 45 Aeff. 45 Aeff. 45 Aeff. 45 Aeff.
Mains input fuse (automatic circuit
breaker)
T15A M3 T12A M3 T10A T1 T10A T1
Secondary fuses F2:T1,6AL250 VAC(6.3x32)
F3:T1,0AL250 VAC(6.3x32)
F4:T6.25AL250 VAC(6.3x32)
Dimensionsandweightspecication
Overall size of device, without
handwheel (width x depth x
height)
600 x 722 x 1206 mm
Overall size of device, with
handwheel (width x depth x
height)
730 x 722 x 1206 mm
Working height (armrest) 1025 mm
Overall size of packaging (width
x depth x height)
960 x 820 x 1400 mm
Empty weight (without
accessories)
135 kg

18 Version 2.0, Revision K
Technical Data
3
Environmentalspecication
Operating altitude Max. 2000 m above sea level
Operating temperature +18 °Cto+35 °C
Relative humidity (operation) 20to60 %RHnon-condensing
Transport temperature -29 °Cto+55 °C
Storage temperature +5 °Cto+50 °C
Relative humidity
(transport / storage)
10to85 %RHnon-condensing
Minimum distance to walls Back:150 mm
Right:300 mm
Left:150 mm
Emmisions and boundary conditions
Overvoltage category
accordingtoIEC 61010-1
II
Pollution degree
accordingtoIEC 61010-1
2
Means of protection
accordingtoIEC 61010-1
Class 1
Degree of protection
accordingtoIEC 60529
IP20
Heat emission 1440 J/s
A-weighted noise level, measured at
1 mdistance
< 70 dB (A)
EMC class B (FCC Rules part 15)
B (CISPR 11, IEC 61326, CAN ICES-3 (A)/NMB)
Warning
Observe (→P.20–4.1Installationsiterequirements)!
Chamber cooling
Temperature range 0 °Cto-30 °C ±3K,adjustablein 1Kincrements,atambient
temperatureof22 °C
Coolingtimeto-30 °C Max.6 h,atstartingpointandambienttemperatureof22 °Cand
60 %relativehumitity
Max. operating pressure 25 bar
Refrigerant* 315 g,refrigerantR-452A*
Defrosting Hot gas defrosting
Manual defrosting Yes
Automatic defrosting Yes
Programmable Yes (hot gas defrost), selectable time
Defrosting intervals 1 defrost in 24 h or manual hot gas defrost
Defrost duration 12 minutes
Automatic turn-off defrost Atchambertemperatureofmorethan-5 °C
Atevaporatortemperatureofmorethan+38 °C

19
Leica CM1520
Technical Data 3
Quick-freeze shelf
Lowest temperature -35 °C (+3/-5K),atchambertemperatureof-30 °Candat
ambienttemperatureof22 °C
Number of freezing stations 8+2
Defrosting Hot gas defrosting
Defrost duration 12 minutes
Automatic turn-off defrost Atchambertemperatureofmorethan-5 °C
Atevaporatortemperatureofmorethan+38 °C
Manual start Yes
Automatic start No
Manual abort Yes
Warning
*)Refrigerantandcompressoroilmustbereplacedbyservicepersonnelqualiedandauthorized
through Leica only.
Microtome
Type Rotary microtome, encapsulated
Section thickness range 2 µmto60 μm
Horizontal specimen feed 25 mm+3 mm
Vertical specimen stroke 59 mm±1 mm
Specimen retraction none
Maximum specimen size 55 mmx55 mmor50 mmx80 mm
Specimen orientation ±8 °(x-,y-axis)
Coarse feed Slow:max.600 μm/s
Fast:min.900 μm/s

20 Version 2.0, Revision K
Installing the Instrument
4
4. Installing the Instrument
4.1 Installation site requirements
Warning
Do not operate the instrument in rooms with an explosion hazard! To ensure proper functioning of
the instrument, it must be set up while maintaining a minimum distance on all sides from walls and
furniture (→P.13–Transportandinstallation).
The place of installation must meet the following requirements:
• The instrument requires an installation area of approx. 600 x 730 mm
• Room volume must be at least 8 m3
• Roomtemperatureconsistently18 °C-35 °C
• Temperaturerangeduringstorage:5 °C-50 °C
• Relativehumidity,maximum60 %(non-condensing)
• Elevation: up to a max. of 2000 m above sea level
Note
Condensation water can form in the instrument if there is an extreme difference in temperature
between the storage location and the installation site and if humidity is high at the same time. In this
case, wait at least four hours before switching on the instrument. Failure to comply with this may
cause damage to the instrument.
• The instrument is designed for indoor use only.
• The power plug/circuit breaker must be freely and easily accessible. The power socket may not be
located further than 2.5 m from the instrument.
• Power supply at a distance no greater than the length of the power cord – an extension cord must NOT
be used.
• Theoormustbevibration-freeasfaraspossibleandhavesucientloadcapacityandrigidityforthe
weight of the instrument.
• Avoidimpacts,directsunlightandexcessivetemperatureuctuations.
• The instrument must be connected to a compatible grounded power socket. Use only the power cord
provided, which is intended for the local power supply.
• Thechemicalstobeusedfordisinfectionarebothhighlyammableandhazardoustohealth.
Therefore the installation location must be well ventilated, and must contain no sources of ignition of
any kind.
• Furthermore, this instrument must NOT be operated directly under the outlet of an air-conditioning
system, since the increased air circulation accelerates icing of the cryochamber.
• The installation location must be protected against electrostatic charge.
Note
Room temperatures and humidity levels in excess of the recommendations above will affect the
cryostat's cooling capacity and the lowest stated temperatures will not be reached.