Thermo Scientific TCF-32 User manual

Continuous Flow Rotor
TCF-32
Instruction Manual english
Analyze •Detect •Measure •Control™
The original copy of this manual is
valid. Thermo Scientific cannot
guarantee the accuracy or
completeness of subsequent copies.

CONTENTS
1. PREFACE ································································································································3
2. SPECIFICATIONS·····················································································································3
3. COMPOSITION·························································································································4
4. USAGE ··································································································································11
4.1 General description on usage ·························································································11
4.2 Usage·······························································································································12
4.3 Capacity in rotor ·············································································································25
4.4 Determination of sample flow rate····················································································26
4.5 Lubricating unit ············································································································· 26
5. PRECAUTIONS IN USING ROTOR······················································································ 28
6. TROUBLE SHOOTING ········································································································· 31
7. LIFE AND WARRANTY OF ROTOR····················································································· 33
8. WARRANTY OF LUBRICATING UNITAND SEALATTACHMENT ASSEMBLY··················· 34
9. MAINTENANCE ······················································································································35
10. SEPARATION CHARACTERISTICS ··················································································· 38
11. COMPONENTS AND ACCESSORIES ················································································ 39
12. INSTRUCTION FOR 940ML CORE······················································································ 44
13. Preventive Maintenance ······································································································ 47

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Precautions on Handling rotor
Do not spin a rotor beyond the allowable speed.
{The maximum speed marked on a rotor refers to an allowable one when the maximum density
of the sample or density gradient solution is less than 1.2.
{When a sample or density gradient solution of more than 1.2 in maximum density is used, the
maximum speed must be reduced according to the instruction manual for the rotor.
{Upon reaching the end of the service life of a rotor specified in its instruction manual, the
allowable speed should be reduced by 10%.
Set a rotor gently and securely onto the drive shaft hub.
{Practice setting repeatedly to acquire the knack.
Confirm that the rotor is free from corrosion and scores.
{If white corrosion is recognized on a tube hole bottom of an angle rotor, or a hole bottom of a
bucket for swing rotor, immediately stop using it and contact the nearest agent of Thermo
Scientific.
{When operation is over, store the rotor after cleaning and drying.
Make it a practice to keep a logbook for rotors.
{Keeping a logbook for rotors is indispensable for controlling the life or when the warranty
clause is involved. Keep the logbook up to date and store it properly.

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Do not rotate this rotor at a speed of more than 3,000 rpm unless all components (rotor
assembly, seal attachment assembly and lubricating unit assembly) are assembled.
Do not rotor at a speed of more than 3,000 rpm unless the rotor is filled with liquid.
If the above cautions are not observed, shaft (D) could be broken or the core could be
damaged.
Always ground the ultracentrifuge, Iubricating unit and low-temperature circulating chamber
before use.
Be sure to read article on 9. MAINTENANCE.

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1. PREFACE
We have developed a continuous flow rotor which permits sizable processing with samples
allowed to flow continuously.
Utilize this continuous flow rotor of high performance and reliability for your studies or
processing
2. SPECIFICATIONS
Table 1shows the specifications of this continuous flow rotor.
Table 1 Specifications
Type of Rotor TCF-32
Max. speed 32,000 RPM
Max. centrifugal force 101,685 Xg
K factor 42
Total capacity 430 ml
Maximum flow rate 150 ml/min (9 l/hr)
Sample pH range PH 4~10
Maximum Dia. of rotor 24 cm
Supply power for lubricating unit AC 100-240V, 1A(50/60Hz)
NOTES
For operation of Model TCF-32, the following apparatus are required besides the standard
configurations of Model TCF-32 and Preparative Ultracentrifuge, which have to be prepared for
installation of Model TCF-32 at customer's site.
1. Sample Loading Pump
Specifications
Flow rate: Has to be as fast as operating flow rate required for separation of samples.
Exhaust pressure: More than 1.5kg/cm2.
Model without pulsating motion is recommended.
2. Low Temperature Circulating Water Cooling Bath
Cooling capacity: More than 300 Kcal/hr (water temp. at 5°C)
(Refrigerator: as strong as 300W)
Circulating Pump
Flow rate: More than 1 l/min. (pressure: 0.5-1 kg/cm2)
3. Density Gradient Pump
Required for density gradient separation.

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3. COMPOSITION
Fig.1 shows the overall construction
Lubricant is supplied
to bearing
Fig.1 Overall construction
Lubricating unit
Ass’y
SealAttachment
Ass’y
Rotor body ass’y
Sample outlet
Sample inlet
Water outlet
Oil discharged from
bearing is sucked
and recovered
Cooling water is
supplied
Oil outlet Oil inlet
Water inlet
Door adapter
Bearing
Bearing housing
Shaft (D)
Bearing housing
Seal body ass’y
Collar
Seal Door
Rotor

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3.1 Rotor body assembly
(1) Rotor and cover
The rotor and cover are made of titanium alloy which is excellent in mechanical strength
and corrosion resistance, and is coated in black (see Fig.2)
(2) Core titanium sleeve and blades
All of them are introduced in the rotor. The core forms a passage of sample and density
gradient solution in the rotor. Its material is Noryl.
The titanium sleeve is for reinforcing the core, and is put on the outside of the core. It is
made of titanium alloy.
The blades are for preventing the sample and density gradient solution in the rotor from
whirling up. It is made of Noryl.
(see Fig. 3)
Fig. 2 Rotor body assembly Fig. 3 Core, titanium sleeve and blades
Core
Blade
Titanium sleeve

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(3) Shaft (D) and Swagelok nut
The shaft (D) is made of titanium alloy. The section sliding with the bearing is coated with
molten tungsten carbide to improve the wear resistance.
The Swagelok nut is made of stainless steel. It fastens the shaft (D) on the cover (see Fig. 4)
Shaft (D) is a critical component. Be careful of handling it so that it is not bent, etc.
(4) Seal tube head and seal
The shaft area of seal tube head is inserted in the (polyphenylene oxide resin).shaft up to
the core, and serves as a passage for sample etc. It is fastened on the shaft with left hand
threads.The material is stainless steel. The seal is mounted on the top of the seal tube head.
It is an important component in charge of letting in and out sample, etc. to and from spinning
rotor. The employed material is polyimid resin whose sealability is excellent at a high
peripheral speed (see Fig. 5 and 6).
Fig. 4 Shaft (D) Fig. 5 Seal tube head Fig. 6 Seal

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3.2 Seal attachment assembly
(1) Door adapter
It is screwed in the door of the ultracentrifuge, and supports the entire seal attachment
(see Fig. 7).
(2) Bearing housing
The bearing housing holds the bearing made of white metal, and is engaged with the rotor
shaft (D). It is supplied with lubricant and cooling water from the peripheral connector (see
Fig. 8).
Fig. 7 Door adapter Fig. 8 Bearing housing
(3) Seal body assembly, collar and screw sockets
The seal body assembly consists of seal piece which is in contact with the rotor seal and
transfers sample, etc., pipe, spring, manifold and connector. The seal body assembly is
pushed against the seal by the spring. As for materials, the seal piece is made of tungsten
carbide and others are made of stainless steel.
The collar which is made of stainless steel is put on the outside of the seal body assembly,
and seals the cooling water which cools the seal body assembly.
The screw sockets (A) and (B) fasten the seal body assembly and collar (see Fig. 9 and
10).

-8-
Fig. 9 Assembly of seal body Fig. 10 Seal body assembly
Assembly, collar and
Screw sockets
3.3 Lubricating unit assembly
The lubricating unit assembly is used when operating this continuous flow rotor.
The specification and operation procedure of this unit are as follows.
(1) Specification
Item Specification Remarks
1. Lubricating oil supply
flow rate 80 ± 20 ml/min lFor the seal attachment assembly lubrication
2. Drain suction
pressure 81 kPa (The pump simple substance pressure).
lFor the DRAIN collection
3. Malfunction detection lLubricating oil flow detection
(The pressure detection
formula)
lCooling water flow detection
(The pressure detection
formula)
lIt is an extraordinary caution
when Lubricating oil doesn't
flow.
lIt is an extraordinary caution
when cooling water doesn’t
flow.
4. Malfunction warning There is a warning in the
electronic buzzer.
5. The container The glass bottle of 500 ml
For lubricating oil and the waste
oil
6. Noise 60 dB (A scale) l1m of the front
7. Input power lInput power :
AC 100-240V, 50/60Hz
lInner supply voltage :
DC 12V, 4.17A max.
lIt uses AC adapter
8. Size 273 X 318 X 219
9. Weight 10.8 kg
Sealbody
ass’y
Collar
Screw
socket (A)
Screw
socket (B)