Bertin Technologies Cryolys Evolution User manual

Document reference: CLYS-800-DU001-A
Original Instructions
Cryolys®is a registered trademark of Bertin Technologies.
© 2017 Bertin Technologies. All rights reserved
For research use only
Document reference: CLYS-800-DU002-A
Translated instructions from the French original version
Cryolys® is a registered trademark of Bertin Technologies.
© 2017 Bertin Technologies. All rights reserved
For Research purposes only

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Restricted User License:
By purchasing or using the Product, the user accepts the terms and conditions of
this License in full.
The purchase or use of the Product confers on the User the non-transferable right
to use the Product for Research purposes only. The term Research denotes any
scientific research programs conducted directly by the User for their own
purposes; the User renounces the right to use the Product for the purposes of
developing a treatment or diagnostic method as well as any use of the Product
for Commercial purposes.
The term "Commercial" includes in particular use of the Product or its
Components, (i) for manufacture, (ii) for the provision of services, information or
data to a Third Party, (e.g. paid sample testing), (iii) for resale, whether within the
context of Research or not, (iv) for quality control or providing quality assurance
for the manufacturer of a commercialized product.
The User is not authorized to modify, resell or transfer the Product or its
components.

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Legal Information:
Bertin Technologies and/or its subsidiaries deny any liability either explicit or
implicit and particularly but not exclusively for commercial or individual
warranties linked to this document.
Except where otherwise provided for by law, neither Bertin Technologies nor its
subsidiaries can be held liable, either in contractual or criminal law, and in terms
of a guarantee or under any other regime that may be, for any damage in
whatever form which may occur particularly but not exclusively as a result of the
use of this document.
Neither Bertin Technologies, nor its employees, can be held liable for losses or
costs resulting from its use.
The information contained in this document is provided solely for use with this
Bertin Technologies product under the terms and conditions of the Restricted
User License. Bertin Technologies is not liable for any use of this information when
applied to other products.
Bertin Technologies reserves the right to modify this document and make any
changes at any time without prior notice.
This liability waiver clause is written and interpreted according to French law. Any
legal action relating to the content of this clause is the exclusive jurisdiction of the
Courts of Law in Paris, France.
© 2017 by Bertin Technologies.
This document is protected and contains information which is the property of
Bertin Instruments. The Bertin Instruments, Precellys® Evolution and Cryolys®
Evolution logos are registered trademarks of Bertin Technologies.

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Table of Contents
............................................................................................................................................1
1Introduction..................................................................................................................5
1.1 Safety instructions and recommendations...................................................5
1.2 Warranty..............................................................................................................9
2Description of the Cryolys®Evolution ....................................................................10
2.1 Description of the equipment.......................................................................10
2.2 Specifications...................................................................................................11
2.3 Generating cold air.........................................................................................12
3Installing the Cryolys®Evolution..............................................................................14
3.1 Unpacking and installation............................................................................14
3.2 Packaging and transport...............................................................................19
3.3 Storage..............................................................................................................20
4Use...............................................................................................................................21
4.1 Description of user interface..........................................................................21
4.2 Starting temperature-controlled homogenization....................................22
5Cleaning and decontamination............................................................................26
5.1 Recommendations..........................................................................................26
5.2 Decontamination protocol............................................................................26
6Support and documentation .................................................................................27
6.1 Frequently asked questions ...........................................................................27
6.2 Troubleshooting................................................................................................28
6.3 Support..............................................................................................................29
6.4 Disposal..............................................................................................................29
7Appendix: Example of CE Declaration.................................................................30
8Appendix: Electrical diagram.................................................................................31

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1INTRODUCTION
Thank you for purchasing a Cryolys®Evolution by Bertin Instruments.
The Cryolys®Evolution is a cooling system specifically designed for the Precellys®
Evolution. Based on the use of cooled atmospheric air through the sublimation of
dry ice, it provides optimal protection for temperature-sensitive molecules such
as proteins, small RNAs or even particular chemical compounds by maintaining a
temperature between 0 and 10°C. The Cryolys®Evolution is the solution for
preserving the molecules in your samples and therefore for obtaining the best
possible results.
This user manual includes all the information you need to use and maintain your
Cryolys®Evolution. The technical specifications of this product, along with all the
information contained in this manual may be modified at any time without prior
notice.
1.1 Safety instructions and recommendations
Read this user manual carefully before using your Cryolys®Evolution.
If you are in any doubt about the safety of the device, please contact us by
email at this address: [e-mail] or contact your local
distributor, whose address is listed on our website: Bertin Instruments.
Bertin Technologies cannot be held liable for any damage or injuries caused by
using this device in a manner other than that described in this manual.

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Reference labels are attached to the device's components. Users must comply
with the information printed thereon.
1.1.1 Risk of electric shock
As the Cryolys®Evolution is connected electrically to the rear panel of the
Precellys® Evolution, usage precautions and recommendations against electric
shocks are as described in the Precellys® Evolution User Manual (02520-800-
DU003-B).
1.1.2 Biological risks
Always wear the necessary personal protection equipment (PPE) when cleaning
any parts soiled by biological samples, for example inside the Precellys®lid.
Note: Refer to the Precellys®Evolution manual (02520-800-DU003-B) as well as to
the procedures currently in place in your laboratory
Eye and face protection
Hand and skin protection

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1.1.3 Risks linked to dry ice
Dry ice (cardice) is made from an accumulation of carbon dioxide in a solid
state with a temperature of -80°C.
It sublimates in air into CO2, a colorless and odorless gas. The principal risks linked
to the handling of this product are asphyxia and burns. In order to protect
yourself against projectiles and the risk of asphyxia, the user must wear protective
gear for the eyes, face and skin. Store and handle dry ice in a ventilated area.
CAUTION! Ensure you are in possession of the safety data sheet covering the risks
and precautions linked to the use of dry ice (handling using protective gloves,
transport and storage under particular conditions, etc.).
1.1.4 Mechanical risks
The lid is liable to self-closure. We recommend keeping it in the open position
with your hand during all operations requiring it to be opened. If not, we
recommend closing it.
When opening the lid, please be aware of the risk of pinching between the
hinge and the rear of the Precellys®Evolution.
Risk of burns: Dry ice at -80°C
Risk of asphyxia
RISK OF
PINCHING

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1.1.5 Special Safety Symbol
Comply with the safety instructions associated with this symbol.
1.1.6 Recommendations
Any use of this device other than that described in this manual can alter its
external protection and damage its internal components.
We recommend that you:
-Do not install unauthorized components or accessories; this can alter the
protection afforded by the device and void its warranty.
-Do not drop the device: the Cryolys®Evolution module must always sit
atop the Precellys®Evolution or on a horizontal surface, or its
components may become damaged.
-Dot not handle the device if it is fully or partially dismounted or if it is
damaged.
-Do not invert the connections as this could irreparably damage the
connectors.
-Do not plug the Cryolys®Evolution in to a power supply other than the
one supplied by Bertin Instruments.
-Do not obstruct the fan.
-ONLY use sticks of dry ice (do not use nitrogen or H2O ice).
-Do not fill the dry ice container beyond its limit (see "4. How to Use")
-Do not ship the device if the container contains dry ice.
-Do not ship the device in anything other than the original packaging.

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1.2 Warranty
The manufacturer's warranty is limited to one (1) year. This warranty is valid from
the date the equipment is registered on our website: (www.bertin-
instruments.com). This covers failure or malfunction of any of the components in
the device where it has been used in compliance with the recommendations
contained in this manual.
The warranty does not apply in the following cases:
- Use or maintenance which does not comply with the instructions contained in
this manual.
- Opening, repair or modification of the device by unauthorized persons.
- Alteration or removal of the label on which the device's serial number is printed.
Manufacturer:
Bertin Technologies
Parc d’activité du Pas du Lac
10bis Avenue Ampère
78180 MONTIGNY-LEBRETONNEUX
FRANCE

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2DESCRIPTION OF THE CRYOLYS®EVOLUTION
The Cryolys®Evolution is a device designed to keep biological samples in the
Precellys®Evolution at a temperature between 0°C and 10°C to avoid any
degradation linked to a temperature increase during homogenization.
The Cryolys®Evolution allows you to:
-Keep the sample temperature below 10°C throughout the
homogenization process.
-Control the temperature from the Precellys®Evolution’s screen.
-Protect temperature-sensitive molecules from degradation.
2.1 Description of the equipment
5
6
4
3
1
2
9
7
8

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1
Main seal: ensures a good seal between the cover and the module.
2
Diffuser Plate: this is attached to the cover and directs air over the dry ice.
3
Cooling module: ensures uniform diffusion of cooled air inside the Precellys®
Evolution.
4
Module cover: Encloses the cooling module.
5
Locking levers: allow removal or locking of the cooling module from/to the
Precellys® lid.
6
Precellys® lid: its fixed base receives the cooling module.
7
Fan: propels atmospheric air through the module. The four-pin connector
must be connected to the back of the Precellys®Evolution.
8
Temperature sensor: measures the temperature inside the Precellys®
Evolution and detects whether the module is attached to the Precellys® lid.
The three-pin connector must be connected to the back of the Precellys®
Evolution.
9
Hinge reinforcement: reinforces the hinge of the Precellys®Evolution in order
to support the Cryolys®Evolution.
2.2 Specifications
Technical specifications
Supply voltage
110 - 230V
Power consumption
<1 kVA
Physical specifications
Width
330 mm
Depth
336 mm
Height
111 mm

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1: The atmospheric air propelled by the fan is cooled as it
travels over the dry ice
2: The cold air is injected into the tube container via the
Precellys® lid
1
2
Figure 1: cooling principle
Weight (empty module)
1.1 kg
Operating temperature
15-30°C
Humidity
15-85% RH
Altitude
< 2,000 m
Operating specifications
Maximum air flow
168 m3/hour
Dry ice reservoir
1.8 kg
Dry ice consumption
Approx. 1.5 kg/30 mins
Minimum dry ice volume
0.5 kg
Noise level
< 60dB
Temperature control
Between 0°C and 10 °C (1°C increments)
2.3 Generating cold air
Cold air is produced and maintained at the temperature set by the user.

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Temperature during homogenization without Cryolys®Evolution
Temperature during homogenization with Cryolys®Evolution
Precellys®Evolution homogenization cycles
Figure 2: Temperature inside the tubes during homogenization with and
without Cryolys®Evolution

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3INSTALLING THE CRYOLYS®EVOLUTION
3.1 Unpacking and installation
It is important to check the delivered package carefully and inform your carrier
of any damage.
3.1.1 Package contents
The package contains the following items:
-One Cryolys®Evolution (module + specific Precellys® lid)
-Two mounting screws attached to the Precellys® lid
-Hinge reinforcement for the Precellys®Evolution
-Cable tie
-USB stick containing Cryolys®Evolution control software
-User manual
-CE compliance declaration.

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3.1.2 Unpacking the package
1. Open the cardboard
box.
2. Pull out the inner box
using the central handle.
3. Open the inner box
and pull out the top foam
protector.
4. Check that all
components are
present.
5. Separate the Cryolys®
Evolution from its bottom
protector, and place it on a
flat surface.
6. Lift the hinge
reinforcement out of
the bottom protector

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3.1.3 Installing the Cryolys®Evolution
Note: Make sure the Precellys® Evolution is disconnected from the power supply before
installation.
Required tool: No5 Allen (hex) key
1. Unscrew the 2 screws
on the cover of the
Precellys®Evolution
2. Detach the hinge and
remove the original
cover
3. First remove the
cooling module and then
place the lid on top of
the Precellys®Evolution
CAUTION - IMPORTANT
Do not hold the Cryolys®Evolution by its cover

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4. Insert the hinge
reinforcement into the
hinge screw holes
5. Gently tighten the 2
screws supplied with the
cover
6. Connect both fan and
temperature sensor
connectors to their
respective connections
at the back of the
Precellys®Evolution.
7. Attach the fan and board interface cables to the hinge reinforcement using the
cable tie provided.
3.1.4 Updating the software
In order to optimize the use of your instrument, we recommend always having
the latest available software version. You can check the installed software
version via the instrument's "settings" menu.

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Registering your product will ensure you are informed when new software
versions are made available for the Precellys® Evolution. This can be done via the
Bertin Instruments website (www.bertin-instruments.com).
1. Insert the USB stick
containing the latest
version of the software.
2. Turn the Precellys®
Evolution on and select
"Data manager (USB)"
from the Menu.
3. Select "Update
software version".
Figure 3: Precellys
®
Evolution "settings" menu

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4. Press OK and wait 60 seconds.
5. The Precellys®Evolution will restart
automatically.
3.2 Packaging and transport
Before transporting the device, you must:
1. Check that there is absolutely no dry ice inside the module
2. Ensure there is no humidity or, if necessary, dry any humid areas
If you have to transport or ship the Cryolys®Evolution, arrange and pack it by
following these steps:
1. Separate the module
from the Precellys® lid.
2. Disconnect both fan
and board interface
connectors from their
respective connections
at the back of the
Precellys®Evolution.
3. Unscrew the 2 screws
on the cover of the
Precellys®Evolution.

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4. Detach the hinge
reinforcement from the
hinge on the Precellys®
Evolution.
5. Lift the lid off the
Precellys®Evolution.
6. Replace the module
on top of the Precellys®
lid and place the entire
unit inside the foam
protector and on the
inner box
7. Place the top foam
protector over the Cryolys®
Evolution
8. Close the inner box
9. Place inside the main
cardboard box using
the handle
3.3 Storage
The device must be stored in a dry location at a temperature between +0°C and
+50°C on a horizontal surface.
The device may be stored after checking the following points:
1. The module is empty
2. The device is dry, clean and decontaminated