Bertin Technologies PRECELLYS Evolution Touch User manual

PRECELLYS®
Evolution Touch
WWW.BERTINTECHNOLOGIES.COM
USER MANUAL
PRECELLYS®Evolution Touch

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PRECELLYS®Evolution Touch
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USER MANUAL
PRECELLYSEVOLUTION
BERTIN TECHNOLOGIES
Parc d’activités du Pas du Lac
10 bis, avenue Ampère
78180 Montigny le Bretonneux
France
Tél : + 33 (0)1 39 30 61 60
Precellys® is a registered trademark of Bertin Technologies.
© 2021 Bertin Technologies All rights reserved
Original instructions
Document reference : 23405-800-DU002-A
DISCLAIMER
Bertin Technologies and/or its subsidiaries disclaim all liability relating to
this document, either explicitly or implicitly, including, but not limited to
commercial warranties or specic warranties.
Unless the law provides otherwise, neither Bertin Technologies nor its
subsidiaries shall be held liable, under tort or contract, and regarding
guarantees, or any system whatsoever, for any damage whatsoever,
including, but not limited to, during the use of this document.
Neither Bertin Technologies nor its employees shall be held liable for
losses and costs resulting from this use.
The information in this document is designed only for use with this Bertin
Technologies product. Bertin Technologies is not liable of any use of this
information that is applied to other products.
Bertin Technologies reserves the right to revise this document and to
make changes without prior notice.
This limitation clause is governed and interpreted in accordance with
French law. Any dispute relating to the content of this clause falls under
the exclusive jurisdiction of the Courts of Paris, France.
OWNERSHIP AND CONFIDENTIALITY
The information, studies, plans and diagrams contained in this document
remain the property of Bertin Technologies and are condential.
The information contained in this document may not be used, in whole
or in part, or disclosed or reproduced without the prior consent of Bertin
Technologies.
CONTENTS
1 INTRODUCTION
2 PRECAUTIONS AND RECOMMENDATIONS
2.1 Safety symbols
2.2 Recap of the advice for using the power supply
2.3 Risk of electric shock
2.4 Biological risks
2.5 Non-ionising radiation
2.6 Risk of use
3 DESCRIPTION OF PRECELLYS® EVOLUTION TOUCH
3.1 Presentation of the equipement
3.2 Characteristics
3.3 Noise level
3.4 Warranty 28
3.5 Manufacturer’s Address
3.6 Technical assistance
3.7 Standardisation requirements
3.8 Device reference 30
4 TRANSPORT AND STORAGE
4.1. Transport
4.2. Storage
5. INSTALLATION
5.1 Unpacking the device
5.2 Installation and connection
6. OPERATINGINSTRUCTIONS
6.1 Samples preparation
6.2 Launching a grinding protocol
7. ALARMS
8. CLEANING AND DESINFECTION
8.1 Recommendations
8.2 Disinfection protocol
9 MAINTENANCE AND SERVICING
9.1 Repair
9.2 Problems encountered
9.3 Wear parts replacement
9.4 Support
10. ELIMINATION
11 APPENDICES :
11.1 Electrical diagram
11.2 Precellys® returns

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Read carefully user manual
before operating.
Lire attentivement la notice avant utilisation.
Read carefully user manual before operating.
Ne pas ouvrir le capot lors du fonctionnement.
Do not open the cover while running.
2 PRECAUTIONS AND RECOMMENDATIONS
This manual must be read carefully by the user before any use of the
PRECELLYS® Evolution Touch homogenizer.
Handling this device without following the instructions set out in this
manual could reduce the level of protection provided by the device.
In the event of non-compliance with the safety instructions set out in
this document, Bertin Technologies disclaims all liability for any resulting
damage to property or physical injury.
Contact the local distributor immediately if there is any doubt as to the
safety of the appliance.
2.1. Safety symbols
The following symbols can be found in various places on the device.
Follow the instructions associated with these symbols.
The equipment may only be repaired by personnel who have been autho-
rized by the dealer or manufacturer.
The waste resulting from normal analysis operations must be disposed of
in biological bins and treated by specialised companies.
This manual must be read carefully by the user before using the PRECELLYS®
Evolution Touch homogenizer.
1. Introduction
Thank you for purchasing a Precellys® Evolution Touch by Bertin
Technologies.
Precellys® Evolution Touch is a universal tissue homogenizer that is both
intuitive to use and eective such that it can be adapted for routine or
R&D projects.
The Precellys® Evolution Touch can homogenize a large number of
dierent samples in between a few seconds and two minutes.
It has a patented automatic tube locking system that uses a vacuum
called «Press and Block» which makes the Precellys® Evolution Touch
safe and easy to handle. Evolving in the eld of Life Sciences, the
Precellys® Evolution Touch is compatible with work environments up
to biosafety level 3 (BSL3). The instruments in the Precellys range are
used by thousands of scientists around the world. The instrument’s
unique 3D motion delivers the same high level of energy and eective
homogenization to each tube.
This manual presents all the information needed for the unpacking,
installation, routine use and maintenance of the Precellys® Evolution
Touch.
The product’s technical specications and the following information are
subject to change without notice.
Earth connection: safety of the electrical installation. By
allowing to reduce the risks of electrocution and electrication.
The sticker with the «Biohazards» symbol is delivered with the
device and must be stuck on the front of the device for use with
potentially infectious samples.

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Heavy load hazard: there is a heavy load sticker on the packaging
of the equipment. The Precellys Evolution Touch should be
carried by two operators, preferably. Safety shoes must be worn
by the operator during transportation.
High temperature hazard: this sticker warns about a hot surface.
The maintenance operator must wait at least 60 minutes after
the end of a cycle before carrying out work on the equipment.
Electrical hazard: before any maintenance operation, the
operator must disconnect the mains supply.
Only use the device with the type of electrical power source
indicated on the label.
Do not pull on the cords. Hold the plug and not the cable when
plugging in or unplugging the cord.
Do not expose the device to sudden variations in temperature
and humidity, avoid shocks, intended for indoor use only.
Do not disassemble or repair the power supply by yourself.
There is a risk of electrocution.
Do not immerse the device, do not use the device in explosive
atmospheres or in wet environments.
Unplug the cord from the wall outlet before cleaning the unit.
The power supply may become hot during use. Before
transporting it, disconnect it and let it cool down.
2.2. Recap of the advice for using the power supply
2.3 Risk of electric shock
Although the device is perfectly insulated and earthed, it is important
that all users are made aware of the risks associated with the use of liquids
near an electrical power supply. If any liquid is spilled, the device must be
immediately disconnected from the power supply by unplugging the mains
plug even if the device is in operation. The device should then be dried and
any spilled liquid should be wiped up.
To avoid any risk of an electric shock, the device’s power supply must be
connected to an installation that complies with the applicable standards.
2.3.1 Fuse
The device contains an external fuse located on the rear panel. If they need to
be changed, they must be replaced with a fuse 5x20 - T 10 A - H 250 V. It can
be easily replaced by the user using a screwdriver (see § 9.3.3).
The means of disconnection of the equipment is the mains
connector.
Disconnecting the power cord from the device cuts o the
power supply to the device.
Do not put the device in a place where it would be dicult to
operate the disconnecting mechanism.
The power cord must not be replaced with a part number other
than the one supplied with the product.
- Do not plug the device back in until it has been
checked.
- The casing must not be removed by the operator
under any circumstances - Risk of electrocution.
If the system does not start, the operator should
contact the manufacturer.
Failure to follow the instructions associated with
the symbol may result in physical injuries and
environmental damages.

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Protective glasses Gloves
2.4. Biological risks
Always wear gloves and protective glasses when handling bio-hazardous
samples and take all necessary precautions to prevent any risk of
contamination. Use the best practices implemented in your laboratory.
2.5. Non-ionising radiation
WARNING:
This device is a Class A apparatus. In a residential environment, this
device may cause radio interference. In such case, the user should take
appropriate measures.
2.6 Risk of use
2.6.1 Use of lysing kits
Depending on the user settings of the PRECELLYS® Evolution Touch
(speed, number of cycles, duration of a cycle, time delay between two
cycles), excessive heat of the lysing tube may lead to their sudden
degradation.
In order to ensure the good working order of the Precellys® Evoution
Touch, it is strongly recommended to use the Lysing Kits produced by
Bertin Technologies and to comply with the operating limits dened for
each Lysing kit. These operating limits specify the maximum levels of the
settings (speed, number of cycles, duration of a cycle, time delay between
two cycles, etc.) that should not be exceeded for the biological samples to
be prepared correctly.
The operating limits of the lysing kits are available on the website www.
bertin-technologies.com.
2.6.2 Incorrect operation
Handling this device without following the instructions set out in this
manual could reduce the level of protection provided by the device.
Bertin Technologies shall be exempt from any claim for compensation for
any damage to property or physical injury resulting from a non-compliance
with the safety instructions set out in this document.
3 DESCRIPTION OF PRECELLYS® EVOLUTION TOUCH
3.1 Presentation of the equipement
3.1.1 Instrument
The PRECELLYS® Evolution Touch is a device designed to lyse and
homogenize biological samples contained in tubes at variable speeds,
in order to extract proteins, nucleic acids, drugs, etc. It simultaneously
processes tubes at high speed which may contain several millilitres of
sample (see the range of tubes oered at www.bertin-instruments.fr).
The PRECELLYS® Evolution Touch oers the following key advantages:
Do not tilt the device: the PRECELLYS® Evolution Touch must
always be resting on its four feet. If not, the internal components
can get damaged or the plastic casing break.
Do not handle the instrument if the casing is partially or
completely disassembled or if it is damaged: high voltage
levels in the device are hazardous.
Do not handle the instrument if the earth connection is
disconnected.
Do not install any unauthorised electronic board, component
or accessories as this may aect the protection provided by the
device and will void the warranty.
Check that the supply voltage specied on the rear panel of the
device matches the voltage of the power supply.
Do not immerse the device.
Easy tube loading: innovative and automated locking system.
Easy decontamination: the areas to be cleaned are very easily
accessible.
Flexible and easy programming cycles (cycle time, speed).
No degradation of the biological material and no cross-
contamination between samples.
Eective and identical grinding in all the tubes and whatever
their volume.

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Figure 1 : Home screen
3.1.4 Présentation de l’interface de commande
The touch screen lights up when the PRECELLYS® Evolution Touch is
powered on and displays the main menu after a few seconds of system
boot up.
Lid: closes the equipment.
Locking handle: locks the Precellys® Evolution Touch lid and
prevents it from opening.
Human Machine Interface: allows the user to program and
start the protocols.
Tube blocking plate: blocks the lysing tubes inserted in the
tube holder.
Tube holder: supports the lysiing tubes.
Metal support: supports the tube holder.
Containment seal: allows the sealing of the Precellys® Evolu-
tion Touch.
Air inlet: diuses the air in the Precellys® Evolution Touch.
USB port: exports/imports data using a USB stick.
Additional 24V DC connection: allows the connection of a
Cryolys Evolution unit.
On/O button: turns the Precellys® Evolution Touch on and o
Power plug: connects the Precellys® Evolution Touch to the
power supply.
Fuse: protects the Precellys® Evolution Touch from power
surges.
Additional 3V - 5V DC connection: allows the connection of a
Cryolys Evolution unit.
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3.1.2 Tube kinetics
Given the design and symmetry of the machine, each of the tubes of
the same type placed in the rack has strictly the same kinetics, which
guarantees the same quality of lysis and homogenization in each of the
tubes.
The centre of gravity of the tubes moves through a complex hemispherical-
shaped pathway. The mixture contained in the tubes therefore undergoes
a three-dimensional motion that prioritizes vertical movements, thereby
ensuring the eciency of the lysis. The movement generated by the
PRECELLYS® Evolution Touch is a precessional motion, which means that
the tubes are not rotated.
The geometry of the tubes used must be compatible with the rack and be
able to withstand accelerations of 600 g for 5 minutes without becoming
deformed (see recommendations for use § 2.6.1).
The precessional motion undergone by the biological samples causes the
temperature of the sample and apparatus to rise.
3.1.3 Description of the equipment

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2
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Protocol parameters Values
Speed* From 4500 to 6800 rpm and from 7200 rpm
to 10 000 rpm increments of 100 rpm
Number of cycles From 1 to 10
Grinding time 5 to 120 s by increment of 1 second
Time delay between 2 periods 5 to 120 s by increment of 1 second
Tube size From 0.3 mL to 50 mL
** Cooling module ON or OFF
** Setpoint temperature From 0 to 10°C by increment of 1°C
** Cooling control mode Manual or automatic
3.1.4.1 Programming
The PRECELLYS® Evolution Touch is designed to operate at a maximum
speed of 10 000 rpm.
A PRECELLYS® Evolution Touch operating protocol is made up of several
successive cycles. Each protocol is composed of dierent parameters
listed in the table here-after and their setting can be made in the“Protocol
settings”or “Protocol library” menus.
* Programming speed between 6900 rpm and 7100 rpm is not permitted,
in order to ensure good functioning and a maximum product life.
** The cooling module is an option.
The operating protocol is under the responsibility of the operator. The
manufacturer recommends for each speed an operating range that
prevents a strong rise in the temperature of the device, the following
paragraph gives the limits of the adjustment parameters accessible to the
operator.
LED: indicates the status of the equipment:
• green: equipment operational and ready to start a cycle
• red: alarms
Screen: displays all the information related to the settings and
faults of the equipment
Start/Stop Button: starts and stops the cycle
1
2
3
Access to the
protocol settings.
Access to the list of
saved protocols
Access to the settings
of the device.
3.1.4.2 Description of the command interface

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3.1.4.3 Setting up protocols on the Precellys® Evolution Touch
The Precellys® Evolution Touch operates at a maximum speed of10000
rpm.
A homogenization protocol for a Precellys® Evolution Touch is made up of
one or more steps between which the lid must not be opened.
The Speed and other settings can be set using the touch screen.
In order to program an homogenization protocol, press the “Protocol
Setup”icon on the home screen. The screen below then appears:
1. Click on an icon on the left side to choose the setting that you want to
modify.
2. An adjustment bar appears on the right of the screen.
3. Change the value by dragging the slider from right to left or by using
the -/+ symbols.
Once all the parameters are set, it is possible to either run the protocol by
pressing the start button on the instrument below the screen, or to save
the protocol in the instrument library.
3.1.4.4 Saving the protocol
To save a protocol, press on the disc icon on the cycles parameters screen
(bottom left of the screen). The screen below then appears:
Protocol settings Icon Values
Grinding time 5 to 120 s
Speed
4500 to 6800 rpm and
7200 to 10 000 rpm,
100 rpm increments
Number of grinding cycles 1 to 10
Pause time between cycles 5 to 120 s
Tube size 0.3 mL to 50 mL
Cooling module ON or OFF
Setpoint temperature From 0 to 10°C in
increments of 1°C
Cooling control mode Manual or Automatic

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1. Enter a protocol name using the virtual keyboard
2. Select icon to be associated with the protocol.
3. Save by using the button, , the protocol is directly added to the list
of the protocols available in the library menu. However, it is possible to
cancel the saving operation and return to the setting part if necessary by
clicking on the bouton .
The operator is responsible for the settings of the equipment. For each
lysing kit, the manufacturer recommends ranges of use.
Note: to prevent the motor from overheating during a long grinding
phase, the Precellys® Evolution Touch has a safety system that prevents
the device from running when the motor temperatures are too high.
Should this occur, let the device cool down before using it.
3.1.4.5 Access to the protocol list
In order to select an existing protocol, press the “Protocol library” icon on
the home screen. The screen below then appears:
1. Global protocol settings overview.
2. Name of the saved protocol.
3. Create a new protocol based on the selected protocol.
By choosing a protocol in the list, (right side of the screen) the associated
parameters are visible (left side of the screen).
Once a protocol selected it is possible:
• To run the protocol by pressing the start button on the instrument
(below the screen). The system creates a vacuum underneath the
indented plate. Once the tubes are correctly held in place (i.e. sucient
vacuum), the protocol begins.
• Edit a protocol by pressing the Edit icon. When the required parameters
of the protocol are set, launch the protocol by pressing the start button of the
instrument (below the screen) or save the modied protocol under another
name.
3.1.4.6 Running protocol
While a protocol is running on the instrument, the following screen is displayed:
Light signal:
- Flashing green: lysing cycle in progress.
- Flashing red: equipment fault message will appear (see
paragraphs 7 for further details) .
3.1.4.7 Protocol end
At the end of a protocol, the following screen appears:
Click Ok on the pop-up message, then open the lid by pinching the locker..
12
3
Green light signal
Cycle number/ total number
of cycle
Cycle running time
Pause time
Total remaining time

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3.1.4.8 Access to the settings of the Precellys Evolution Touch device
In order to modify the setting of the device, press the setting icon on the
home screen. The screen below then appears:
3.1.4.8.1 Preference
3.1.4.8.1.1 General
In order to Adjust the settings, press the Preferences icon on Parameters
screen. The screen below then appears:
1. Adjust the luminosity of the screen by moving the adjustment bar.
2. Click on the icon of the appropriate language. The choice of language is
eective the next time the equipment is switched on (French, English and
Spanish languages are available).
3. Set the time and date.
4. Press the top right arrow to back to the settings screen.
3.1.4.9 Access to maintenance
The Precellys® Evolution Touch is composed of wearing parts that must
be replaced regularly at least according the dened intervals (see § 9.3)
or before if the intensive use of the device leads to a visible wear of parts.
This menu is composed of a list of spare parts and species the maintenance
actions that users must perform on a regular basis in order to ensure the
continuous good working order of Precellys® Evolution Touch:
The rst indicator shows the number of days since the part was replaced.
The second indicator shows the number of cycles performed with the
part.
When any of the indicators exceed the recommended maximum, The tool
icon in the top bar panel. The operator must then proceed to replace
the part (§9.3) or contact the local distributor.
Press the top right arrow to return to the Settings menu.
For any other issues, refer to paragraph 9 or contact your local
distributor.
3.1.4.10 Data exchange :
In order to exports/imports data using the USB port (located on the rear
panel of the equipment), press the Data exchange icon on Parameters
screen. The screen below then appears:

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1. Click on “Export logs to USB” icon to back up the equipment’s le
history: protocols launched, alerts, date changes
2. Click on the «Export library to USB» icon to save the list of protocols of
the equipment.
3. Click on the «import library from USB» icon to replace the library of the
equipment with the one saved on the USB stick.
WARNING: this action will delete all the protocols registered in the
unit.
Press the arrow to go back to the Parameters screen then press the home
icon to back to home screen.
3.2 Characteristics
3.3 Noise level
In normal operation (rotation at 10 000 rpm), the device emits a noise
level lower than 70 dB.
3.4 Warranty
Bertin Technologies warrants that the equipment is free of defects when it
is shipped.
This warranty is limited to a period of one (1) year and does not include
the following parts: fuses, anti-rotation kit, vacuum and containment seals,
blocking plate and tube holder.
This warranty begins when the equipment is installed and registered online
on the www.bertin-technologies.com .
It does not apply in the following cases:
3.5 Manufacturer’s Address
Bertin Technologies
Parc d’activité du Pas du Lac
10 bis, avenue Ampère
78180 MONTIGNY-LE-BRETONNEUX
France
3.6 Technical assistance
If you cannot resolve a problem after reading this manual, contact the
local oce of your dealer.
Technical characteristics
Supply voltage and frequency 110 V~ / 230 V~ (+/- 10%)- 50 Hz / 60 Hz
Consumption <1 kVA
Fuse 5x20 – T 10A – H 250
Button battery Panasonic CR-2032/BN : 3Vdc – 225 mAh
Safety Class I device
Physical characteristics
Width 380 mm
Depth 520 mm
Height 400 mm (635 mm with the lid open)
Mass 27.2 kg
Temperature of use and storage 15-30°C
Humidity 50-85% RH
Altitude <2,000 m
Noise <70 dB
Pollution level No. 2: standard environment
Speed 4500 –10000 tr/min
Number of periods 1 - 10
Duration of period 10 – 90 secondes
Time delay between two periods 1 – 120 secondes
Operating characteristics
User interface
Keyboard 1 push Button
Display 1 x 5-inch LCD capacitive touchscreen
1 indicator (green and red)
Capacity
Number of tubes 96 24 12 6 3
Total volume of a tube 0.3ml 0.5 ml/2 ml 7 ml 15 ml 50 ml
The equipment has not been installed, operated or maintained
in accordance with the instructions described in this manual.
The equipment has been repaired or altered by unqualied
personnel.
The serial number of the equipment is damaged or removed.

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3.7 Standardisation requirements
This equipment complies with the requirements of the CE marking except
for requirement 6.3.2 b) of IEC 61010-1. However, with respect to this
requirement, the equipment complies with the Low Voltage Directive and
the standard governing IT equipment EN 62368-1.
3.7.1 Intensive operation
If the device is used intensively (high speed, long cycle times, full tube
load, sequenced cycles), the normal rise in the overall temperature of
the system may trigger the thermal safety device. In this case, the power
supply to the motor is automatically cut o in order to prevent the device
from overheating.
3.8 Device reference
Reference of Precellys® Evolution Touch: 23405-300-RD001.
4. TRANSPORT AND STORAGE
4.1 Transport
Avoid violent shocks which could make the device not work properly.
Do the following each time you transport the device:
1- Reposition the tube holder locking foam.
2- Close the lid.
3- Use the items from the original packaging.
4.2 Storage
The device must be stored in a dry place and at a temperature between +10°C
and +40°C.
5 INSTALLATION
5.1 Unpacking the device
1. Check the contents of the box using the following list:
Should the delivery be incomplete, contact your local dealer immediately.
* A suitable adapter or cable (not supplied) is required in countries using power
outlets other than: Europe, United Kingdom, United States, Switzerland and
Australia.
1 user manual (French OR English)
1 PRECELLYS® Evolution Touch (including 1 blocking plate, 1
vacuum joint and 1 containment seal)
1 mains cable*
1 replacement vacuum joint
1 replacement mains fuse
1 individual quality control form
1 CE certicate
1 manufacturer’s inspection certicate
Do not connect the device to the power supply until
you have completed the installation.
Do not tilt the device: the Precellys® Evolution Touch
must always be resting on its four feet to avoid
damaging internal components or breaking the plastic
casing.

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2.Carefully remove the Precellys® Evolution Touch from its box and place
it on a clean, horizontal and stable work surface (weight of the device
= 27 kg): the height of the 4 feet can be adjusted in order to make the
equipment more stable.
Given its mass, the device should preferably be handled by two operators.
3.Check its external appearance. Immediately report any defect to the
carrier.
4. Keep the original carton box, the protective foam pads and the original
documentation. These items will be needed in the event of a return.
5.2 Installation and connection
1.Remove the foam pad from the tube holder and replace the containment
seal if necessary (voir § 9.3.2).
2.Fit the vacuum joint (voir §9.3.1).
Do not lift the device by holding the lid when
removing it from its box. It must be lifted either using
the extraction handles provided or by holding the
equipment from underneath at the notches provided
on the side of the casing (see paragraph 2.1). There are
also notches provided in the protective foam.
In the event of a signicant temperature dierence between
the storage area and the laboratory, allow the device to
return to room temperature to avoid any condensation.
Keep the foam pad, which must be put back in place
before transporting the device. If the device is returned
to the dealer or manufacturer without this protection,
the warranty will not apply.
The containment seal must be tted in accordance with
the instructions in this manual in order to adequately
protect the operator. Indeed, the containment seal is
an important element of the system used to contain
the electrical parts of the device and protect them
from getting sprayed by liquids.

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3.Check that the technical characteristics of the device provided in this
manual are consistent with the voltage of your power supply (voir §3.2).
4.Check that the air intake of the fan and the base of the casing are
unobstructed: the air outlet is located under the device.
5.Connect the Precellys® Evolution Touch to the mains power using the
appropriate mains cable.
6 OPERATINGINSTRUCTIONS
6.1 Samples preparation
The samples must be prepared in the lysing kits recommended by Bertin
Technologies listed on www.bertin-technologies.com website.
Only the lysing kits recommended by Bertin Technologies should be used.
Using any other accessories may reduce the level of protection provided by
the device.
6.2 Launching a grinding protocol
6.2.1 Switching on the equipment
After connecting the device, the power is turned on using the On/O switch
located on the rear panel, near the power cable terminal.
A few seconds after starting the device, the main menu is displayed. The
operator can choose a stored program from the library or program the cycle
directly in the cycle setup page. (voir paragraphe 3.1.4.3).
6.2.2 Opening the lid
To open the lid: lift the locking handle and tilt the lid as far as the rear limit
stop.
6.2.3 Loading the tubes
The tubes containing the samples are held in the tube holder by the collar
and are retained by the blocking plate that must be removed to load or
unload the tubes.
The holder is removable and designed in such a way to allow it to stand on
its feet even when fully loaded with tubes. It is also possible to operate the
equipment without fully loading the holder.
A vacuum system keeps the blocking plate pressed down on the tube
holder. The branches of the blocking plate hold the tubes in place when this
system is activated (at the start of a cycle) and release them when there is
no longer any vacuum (a few seconds after stopping a cycle).
Using an incompatible power supply can irreversibly
damage the electronics of the device.
Leave about 10 cm of clearance around the air inlets
and outlets.
This device must be powered by a mains outlet with a
protective earth terminal.
Wearing gloves and taking all necessary precautions
according to the risk of infection are recommended
when using the device and handling samples.
Never open the lid if the tube holder is in motion.
If the lid is opened during a grinding protocol, the
protocol is automatically stopped.

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Figure 2 : 2mL holder and
associated blocking plate
example
1.Removing the blocking plate
2.Place the holder on the instrument and place tubes on the holder.
3. Replace the blocking plate
6.2.4 Closing the lid
Tilt the lid until the locking handle engages in its clasp.
6.2.5 Running a grinding protocol
After conrming the start of a grinding protocol with the «Start» button, the
vacuum system lowers the blocking plate.
When the tubes are correctly locked in place (when the pressure is low
enough), the protocol begins and the green light ashes.
The timer (in seconds) displays the time remaining before the complete end
of the programmed protocol.
6.2.6 End of the grinding protocol
At the end of a grinding protocol, the system returns to the «Protocol
settings» menu. The vacuum under the blocking plate stops a few seconds
after the system stops: the blocking plate cannot be removed from the tube
holder as long as the vacuum is not fully released.
Note :
The time and speed cannot be changed when the instrument is running.
6.2.7 Stopping the grinding protocol
During the cycle, the user can stop the current cycle at any time by pressing
the «Stop» button.
Note: When loading tubes, ensure that:
The vacuum joint is correctly positioned in its slot on the holder.
The blocking plate is centered on the holder and rests on the
vacuum joint. A coding device helps to put it in place.
Wait for the system to come to a complete stop before
opening the lid.
In order to avoid overheating of the device, the
Precellys® Evolution Touch is equipped with a safety
system that prevents the device from operating at
high motor temperatures. If this happens, let the
device cool down before using it.
Wait for the system to come to a complete stop before
opening the lid.

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After the user has stopped the cycle, the display shows the remaining
time of the grinding protocol. Pressing the «Start» button allows the
cycle to continue. Pressing the OK button on the screen cancels the cycle
and the screen returns to the «Settings Protocol» or «Library» menu. The
vacuum under the blocking plate stops a few seconds after the system
stops: the blocking plate cannot be removed from the tube holder during
this period.
6.2.8 Operating limits of the grinding kits
The lysing kits produced by Bertin Technologies have dierent usage
limitations and must be used within these limits. The litis are indicated on
the www.bertin-instruments.fr website.
7 ALARMS
Before any maintenance and servicing operation, the
device must be cleaned and disinfected in accordance
with the risk of infection associated with the samples
treated and with the protective equipment required by
the rules in force. After any maintenance and servicing
operation, check that the device is safe.
The list of the main problems which can occur during operation and the
measures to resolve them are set out in the following table:
If one of these faults persists, contact the technical team.
Alarm messages Possible cause(s) Remedial measures
The tubes recommended by
Bertin Technologies are incorrectly
positioned on the tube holder
1.Reposition the tubes
2.Press in the centre of the blocking
plate during the vacuum process
(lid open).
The blocking plate or the tube hol-
der are not positioned correctly.
Put the blocking plate or tube holder
back in position on the tube holder,
making sure that the blocking
plate is correctly guided by the
mistake-proong key.
The vacuum joint is incorrectly
positioned.
Put the vacuum joint back in place
on the metal holder making sure
the joint is sitting properly inside its
groove.
Pressure error
Alarm messages Possible cause(s) Remedial measures
Pressure error The blocking plate is damaged. Replace the blocking plate.
The vacuum joint is damaged. Replace the vacuum joint.
The vacuum circuit is defective. 1.Switch o the device.
2.Contact the technical support.
The lid is not properly closed.
1.Check that nothing is preventing
the lid from closing.
2.Press the lid down and make sure
that the locking handle is properly
engaged in the clasp of the housing.
The detection system is defective.. 1.Switch o the device.
2.Contact the technical support..
The temperature of the motor has
reached its safe limit
1.Leave the device on so that the
ventilation system can operate.
2.Make sure that the fan air inlets/
outlets are unobstructed.
3.If, after 30 minutes of ventilation,
the alarm is still on in the display,
contact the technical support..
The power supply to the system is
unsuitable.
Check that the supply voltage
specied on the rear panel of the
device matches the voltage of the
power supply.
The speed control or the detection
system is faulty..
1.Switch o the device.
2.Contact the technical support.
Pressure error
Lid error
Motor error
Using the lysing kits beyond the operating limits can
lead to the sudden deterioration of the tubes.

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9 MAINTENANCE AND SERVICING
9.1 Repair
The equipment must be cleaned and decontaminated in accordance with
the procedure described in § 8.2 before shipment for repair.
The equipment must be sent with a return form indicating clearly, at the
very least, the procedure used, the persons involved and the date on which
cleaning and/or decontamination was performed. (see appendix 1).
If the return form is missing from the consignment, Bertin Technologies
after-sales team reserves the right to refuse to perform repairs.
9.2 Problems encountered
8 CLEANING AND DESINFECTION
8.1 Recommendations
For safety reasons, and to avoid damaging the equipment, the following
recommendations must be observed:
The exterior of the equipment can be cleaned with a sponge or cloth that
has been moistened with water or 70% alcohol.
8.2 Disinfection protocol
Should a tube burst during operation, for example, the parts likely to be
contaminated by infectious agents must be disinfected with a suitable
disinfectant.
In case of possible contamination of the instrument, the external parts
of the instrument (shell, cover, screen, grinding housing) can be cleaned
with a cloth moistened with water and then decontaminated with a
cloth moistened with a bleach solution (1000ppm/6°Cl), or an anionic
disinfectant. The use of ethanol can be considered but should be avoided
on rubber seals.
The user shall assume full responsibility for their choice of disinfection
process.
If another disinfection process must be implemented, contact the
technical support service beforehand in order to conrm that the
procedure is compatible with the equipment.
Do not spray liquids directly onto the device, especially the
electrical connectors and the openings of the casing (vents and
air inlet).
Unplug the equipment before cleaning.
Do not use a scraper sponge: risk of damage to the device.
Do not use caustic soda or acetone: the device will be
irreversibly damaged.
Immediately remove any liquid from the equipment with a dry
cloth. Encountered issues Possible cause(s) Remedial measures
There is no power at the mains
socket.
1.Check the mains voltage.
2.Check that the voltage supplied by
the mains matches that of the device.
3.Check the connection of the device
to the mains.
The fuse is defective. Replace the fuse.
The ventilation system is defective. 1.Switch o the device .
2.Contact the technical support..
There is no power at the mains
socket.
1.Check the mains voltage.
2.Check that the voltage supplied
by the mains matches that of the
device.
3.Check the connection of the
device to the mains..
The fuse is defective. Replace the fuse..
The display system is defective. 1.Switch o the device.
2.Contact the technical support.
The cap was screwed on incorrectly
or the tube is defective.
The tube used is not a Precellys® tube
The operating limits were not
respected
For safety reasons, the blocking
plate stays locked in position by the
vacuum system.
Switch the device back on in order to
remove the blocking plate.
The fan is not turning.
The screen does not turn on.
A tube is no longer sealed.
If the product contained in the tube
is hazardous or potentially hazardous,
perform the appropriate disinfection
procedure.
Power cut

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9.3.1 vacuum joint replacement
The vacuum joint located on the metal holder wears
out during operation. It is recommended to change it
when the «Pressure error» alarm appears permanently or
recurrently. As a preventive measure, this seal should be
replaced at least once every 6 months.
9.3.2 Containment seal replacement
The containment seal should be changed at least once a year. The tube
holder does not have to be dismantled when replacing it.
Switch o the appliance and unplug
the power cord before carrying out
this operation.
1. Place the inner groove of the containment seal over the
metal ange.
2. Place the outer groove of the containment seal on
the housing. The containment seal should be changed
at least once a year. This procedure does not require the
dismantling of the metallic support ring.
9.3.3 Fuse replacement
Switch o the appliance and unplug the power cord
before carrying out this operation.
Remove the fuse holder by pinching the retaining clips .
9.3 Wear parts replacement
This paragraph species the maintenance that users must perform on a
regular basis to ensure that the Precellys® Evolution Touch works properly
and that the tubes are maintained during the homogenization phase:
The voltage levels in the device are hazardous. In order
to guarantee the safety of the user, including during the
cleaning and disinfection phases, the containment seal
must not be damaged (puncture, tear, etc.)
DO NOT tilt the device: the Precellys® Evolution Touch must always be
resting on its four feet. If not, the internal components can get damaged
or the plastic casing will break.
Wearing part Reference Frequency of
replacement Why ?
2 mL blocking plate +
tube holder P002274-PEVT0-A.0 when damaged
Necessary for maintaining
tubes in place during
homogenization.
7 mL blocking plate +
tube holder P000911-PEVT0-A.0 when damaged
Necessary for maintaining
tubes in place during
homogenization.
15 mL blocking plate
+ tube holder P000810-PEVT0-A.0 when damaged
Necessary for maintaining
tubes in place during
homogenization.
Vacuum joint P000806-PEVT0-A.0 6 months or when
damaged
Necessary for maintaining
tubes in place during
homogenization.
Containment seal P000805-PEVT0-A.0 1 year or when
damaged
Necessary to guarantee the
tightness of the device.
Mains fuse P002574-PEVT0-A.0 when damaged
Anti-rotation kit P002378-PEVT0-A.0 1 year or when
damaged
Necessary for maintaining
tubes in place during
homogenization.
at screwdriver

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9.4 Support
If any information is not present in this manual, contact your local
distributor.
For the latest information on our services, you can consult the www.
bertin-technologies.com page.
You can contact the Bertin Instruments team at the following address:
[e-mail]
spare fuse
Fuse
10 ELIMINATION
The cleaning and disinfection procedure is mandatory before disposing
of the equipment in order to protect people and the environment.
The equipment, its spare parts and its consumables are to be sorted
and recycled in accordance with the directive on waste electrical and
electronic equipment (WEEE) 2012/19/EU.
Bertin Technologies is responsible for processing WEEE
for its products sold in France (via RECYLUM). For
other countries, the importer is responsible for waste
processing.
11 APPENDICES :
11.1 Electrical diagram
11.2 Precellys® returns
11.2.1 Precellys® Evolution Touch decontamination
Before any return, decontaminate the device (see §8.2) and attach the
decontamination certicate.

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11.2.2 Preparing Precellys® Evolution Touch
11.2.3 Positioning the original packaging
11.2.4 Packing
1. Open the lid and
place the black foam
padding inside.
5. Place the blocking
plate and tubes
together as in the gure
and place them into
the special cutout of
the top foam.
1. Close the box with
strong packing tape
when ready.
2. Place the box on a
pallet and secure it with
strapping.
1. Place the cardboard
inserts on a at surface
and position the
bottom foam at its
center.
3. Place the Precellys®
Evolution Touch in the
box using the handles.
Close the lid
2. Place the Precellys®
Evolution Touch in its
space, while ensuring that
the housing and feet are
fully inserted into the foam.
4. Place the upper foam.
Do not hold the device by the lid when putting it in
its box. It must be held using either the extraction
handles provided or by holding the equipment from
underneath at the notches provided on the side of the
casing. There are also notches provided in the lower
protective foam pad.
Given its mass, the device should preferably be
handled by two operators.
Handle the device with care as signicant shocks can
damage the equipment.