Bertin Technologies Cryolys Evolution User manual

CRYOLYS
Evolution
WWW.BERTINTECHNOLOGIES.COM
USER MANUAL
CRYOLYS Evolution

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CRYOLYS®Evolution
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USER MANUAL
PrecellysEvolutionTouch
BERTIN TECHNOLOGIES
Parc d’activités du Pas du Lac
10 bis, avenue Ampère
78180 Montigny le Bretonneux
France
Tél : + 33 (0)1 39 30 61 60
Cryolys® is a registered trademark of Bertin Technologies.
© 2024 Bertin Technologies All rights reserved
Translated instructions from the French original version
Document reference : CLYS-800-DU002-A
RESTRICTED USER LICENSE:
By purchasing or using the Product, the user accepts the terms and conditions of
this License in full.
The purchase or use of the Product confers on the User the non-transferable right
to use the Product for Research purposes only. The term Research denotes any
scientic research programs conducted directly by the User for their own purposes;
the User renounces the right to use the Product for the purposes of developing a
treatment or diagnostic method as well as any use of the Product for Commercial
purposes.
The term «Commercial» includes in particular use of the Product or its
Components, (i) for manufacture, (ii) for the provision of services, information or
data to a Third Party, (e.g. paid sample testing), (iii) for resale, whether within the
context of Research or not, (iv) for quality control or providing quality assurance for
the manufacturer of a commercialized product.
The User is not authorized to modify, resell or transfer the Product or its
components.
LEGAL INFORMATION:
Bertin Technologies and/or its subsidiaries deny any liability either explicit or
implicit and particularly but not exclusively for commercial or individual warranties
linked to this document. Except where otherwise provided for by law, neither Bertin
Technologies nor its subsidiaries can be held liable, either in contractual or criminal
law, and in terms of a guarantee or under any other regime that may be, for any
damage in whatever form which may occur particularly but not exclusively as a
result of the use of this document.
Neither Bertin Technologies, nor its employees, can be held liable for losses or costs
resulting from its use. The information contained in this document is provided
solely for use with this Bertin Technologies product under the terms and conditions
of the Restricted User License. Bertin Technologies is not liable for any use of this
information when applied to other products. Bertin Technologies reserves the right
to modify this document and make any changes at any time without prior notice.
This liability waiver clause is written and interpreted according to French law. Any
legal action relating to the content of this clause is the exclusive jurisdiction of the
Courts of Law in Paris, France.
© 2017 by Bertin Technologies.
This document is protected and contains information which is the property of
Bertin Instruments. The Bertin Instruments, Precellys® Evolution Touch and Cryolys®
Evolution logos are registered trademarks of Bertin Technologies.
CONTENTS
1 INTRODUCTION
1.1 Safety instructions and recommandations
1.2 Warranty
2 DESCRIPTION OF THE CRYOLYS® EVOLUTION
2.1 Description of the equipment
2.2 Specications
2.3 Generating cold air
3 INSTALLING THE CRYOLYS® EVOLUTION
3.1 Unpacking and installation
3.2 Packaging and transport
3.3 Storage
4 USE
4.1. Description of user interface
4.2. Starting temperature-controlled homogenization
5. CLEANING AND DECONTAMINATION
5.1 Recommendations
5.2 Decontamination protocol
6. SUPPORT AND DOCUMENTATION
6.1 Frequently asked questions
6.2 Troubleshooting
6.3 Support
6.4 Disposal
7. APPENDIX: EXAMPLE OF CE DECLARATION
8. APPENDIX: ELECTRICAL DIAGRAM

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Read carefully user manual
before operating.
1.1.2. Biological risks
Always wear the necessary personal protection equipment (PPE) when
cleaning any parts soiled by biological samples, for example inside the
Precellys® lid.
Note: Refer to the Precellys® Evolution Touch manual (23405-800-DU002-A)
as well as to the procedures currently in place in your laboratory
1.1.3. Risks linked to dry ice
Dry ice (cardice) is made from an accumulation of carbon dioxide in a solid
state with a temperature of -80°C.
It sublimates in air into CO2, a colorless and odorless gas. The principal risks
linked to the handling of this product are asphyxia and burns. In order to
protect yourself against projectiles and the risk of asphyxia, the user must
wear protective gear for the eyes, face and skin. Store and handle dry ice in
a ventilated area.
1. Introduction
Thank you for purchasing a Cryolys® Evolution by Bertin Instruments.
The Cryolys® Evolution is a cooling system specically designed for the
Precellys® Evolution Touch. Based on the use of cooled atmospheric air
through the sublimation of dry ice, it provides optimal protection for
temperature-sensitive molecules such as proteins, small RNAs or even
particular chemical compounds by maintaining a temperature between
0 and 10°C. The Cryolys® Evolution is the solution for preserving the
molecules in your samples and therefore for obtaining the best possible
results.
This user manual includes all the information you need to use and
maintain your Cryolys® Evolution. The technical specications of this
product, along with all the information contained in this manual may be
modied at any time without prior notice.
1.1. Safety instructions and recommendations
Read this user manual carefully before using your Cryolys® Evolution.
If you are in any doubt about the safety of the device, please contact us
by email at this address: [email protected] or contact
your local distributor, whose address is listed on our website: Bertin
Instruments.
Bertin Technologies cannot be held liable for any damage or injuries
caused by using this device in a manner other than that described in this
manual.
Reference labels are attached to the device’s components. Users must
comply with the information printed thereon.
1.1.1. Risk of electric shock
As the Cryolys® Evolution is connected electrically to the rear panel of
the Precellys® Evolution Touch, usage precautions and recommendations
against electric shocks are as described in the Precellys® Evolution Touch
User Manual (23405-800-DU002-A).
Eye and face protection
Risk of burns:
Dry ice at -80°C
Hand and skin protection
Risk of asphyxia
Ensure you are in possession of the safety data sheet
covering the risks and precautions linked to the use
of dry ice (handling using protective gloves, transport
and storage under particular conditions, etc.).

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1.1.4 Mechanical risks
The lid is liable to self-closure. We recommend keeping it in the open
position with your hand during all operations requiring it to be opened. If
not, we recommend closing it.
When opening the lid, please be aware of the risk of pinching between
the hinge and the rear of the Precellys® Evolution.
1.1.5 Special Safety Symbol
Comply with the safety instructions associated with this symbol.
1.1.6 Recommendations
Any use of this device other than that described in this manual can alter
its external protection and damage its internal components.
We recommend that you:
RISK OF
PINCHING
Do not install unauthorized components or accessories; this
can alter theprotection aorded by the device and void its
warranty.
Do not drop the device: the Cryolys® Evolution module must
always sitatop the Precellys® Evolution or on a horizontal
surface, or itscomponents may become damaged.
Dot not handle the device if it is fully or partially dismounted
or if it isdamaged.
Do not invert the connections as this could irreparably damage
theconnectors.
Do not plug the Cryolys® Evolution in to a power supply other
than theone supplied by Bertin Instruments.
Do not obstruct the fan.
ONLY use sticks of dry ice (do not use nitrogen or H2O ice).
Do not ll the dry ice container beyond its limit (see «4. How
to Use»)
Do not ship the device if the container contains dry ice.
Do not ship the device in anything other than the original
packaging.
1.2. Warranty
The manufacturer’s warranty is limited to one (1) year. This warranty is valid
from the date the equipment is registered on our website: (www.bertin-ins-
truments.com). This covers failure or malfunction of any of the components
in the device where it has been used in compliance with the recommenda-
tions contained in this manual.
The warranty does not apply in the following cases:
Manufacturer:
Bertin Technologies
Parc d’activité du Pas du Lac
10bis Avenue Ampère
78180 MONTIGNY-LEBRETONNEUX
FRANCE
2. DESCRIPTION OF THE CRYOLYS® EVOLUTION
The Cryolys® Evolution is a device designed to keep biological samples in
the Precellys® Evolution at a temperature between 0°C and 10°C to avoid
any degradation linked to a temperature increase during homogenization.
The Cryolys® Evolution allows you to:
Use or maintenance which does not comply with the
instructions contained inthis manual.
Opening, repair or modication of the device by unauthorized
persons.
Alteration or removal of the label on which the device’s serial
number is printed.
Keep the sample temperature below 10°C throughout
thehomogenization process.
Control the temperature from the Precellys® Evolution’s screen.
Protect temperature-sensitive molecules from degradation.

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Weight (empty module) 1.1 kg
Operating temperature 15-30°C
Humidity 15-85% RH
Altitude < 2,000 m
Operating specications
Maximum air ow 1.8 kg
Dry ice reservoir ON or OFF
Dry ice consumption Approx. 1.5 kg/30 mins
Minimum dry ice volume 0.5 kg
Noise level < 60dB
Temperature control Between 0°C and 10 °C (1°C increments)
2.1. Description of the equipment
1
2
3
4
8
9
5
6
7
Main seal: ensures a good seal between the cover and the
module.
Diuser Plate: this is attached to the cover and directs air over
the dry ice.
Cooling module: ensures uniform diusion of cooled air inside
the Precellys® Evolution.
Module cover: Encloses the cooling module.
Locking levers: allow removal or locking of the cooling mo-
dule from/to the Precellys® lid.
Precellys® lid: its xed base receives the cooling module.
Fan: propels atmospheric air through the module. The four-
pin connector must be connected to the back of the Precellys®
Evolution.
Temperature sensor: measures the temperature inside
the Precellys® Evolution and detects whether the module is
attached to the Precellys® lid. The three-pin connector must be
connected to the back of the Precellys® Evolution.
Hinge reinforcement: reinforces the hinge of the Precellys®
Evolution in order to support the Cryolys® Evolution.
1
2
3
4
5
6
7
8
9
2.2. Specications

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Figure 1: cooling principle
Figure 2: Temperature inside the tubes during
homogenization with and without Cryolys® Evolution
3. INSTALLING THE CRYOLYS® EVOLUTION
3.1. Unpacking and installation
It is important to check the delivered package carefully and inform your
carrier of any damage.
3.1.1. Package contents
The package contains the following items:
3.1.2. Unpacking the package
2.3. Generating cold air
Cold air is produced and maintained at the temperature set by the user.
The atmospheric air propelled by the fan is cooled as it travels over the
dry ice
The cold air is injected into the tube container via the Precellys® lid
Temperature during homogenization without Cryolys® Evolution
Temperature during homogenization with Cryolys® Evolution
Precellys® Evolution homogenization cycles
1
2
1
2
One Cryolys® Evolution (module + specic Precellys® lid)
Two mounting screws attached to the Precellys® lid
Hinge reinforcement for the Precellys® Evolution
Cable tie
USB stick containing Cryolys® Evolution control software
User manual
CE compliance declaration
1. Open the cardboard box.
4. Check that all
components arepresent.
2. Pull out the inner boxusing
the central handle.
5. Separate the Cryolys®
Evolution from its bottom
protector, and place it on aat
surface.
3. Open the inner boxand pull
out the top foam protector.
6. Lift the hinge reinforcement
out ofthe bottom protector

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Figure 3: Precellys® Evolution Touch «settings» menu
Precellys Evolution Touch
-
3.1.3. Installing the Cryolys® Evolution
Note: Make sure the Precellys® Evolution Touch is disconnected from the
power supply before installation.
Required tool: No 5 Allen (hex) key
CAUTION - IMPORTANT
Do not hold the Cryolys® Evolution by its cover
1. Unscrew the 2 screwson
the cover of thePrecellys®
Evolution
2. Detach the hinge and
remove the originalcover
3. First remove the cooling
module and thenplace the
lid on top ofthe Precellys®
Evolution
4. Insert the hinge
reinforcement into
the hinge screw holes
7. Attach the fan and board interface cables to the hinge
reinforcement using thecable tie provided.
5. Gently tighten the 2
screws supplied
with the cover
6. Connect both fan and
temperature sensor connectors to
their respective connections at the
back of thePrecellys® Evolution.
3.1.4. Updating the software
In order to optimize the use of your instrument, we recommend always
having the latest available software version. You can check the installed
software version via the instrument’s «settings» menu.

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Registering your product will ensure you are informed when new software
versions are made available for the Precellys® Evolution Touch. This can
be done via the Bertin Technologies website (www.bertin-technologies.
com).
3.2. Packaging and transport
Before transporting the device, you must:
1.Check that there is absolutely no dry ice inside the module
2.Ensure there is no humidity or, if necessary, dry any humid areas
If you have to transport or ship the Cryolys® Evolution, arrange and pack
it by following these steps:
1. Insert the USB stick
containing the latestversion
of the software.
1. Separate the module
from the Precellys® lid.
4. Detach the hinge
reinforcement from
the hinge on the
Precellys®Evolution Touch.
7. Place the top
foamprotector over the
Cryolys®Evolution.
2. Turn the
Precellys®Evolution Touch
on and select»Data manager
(USB)»from the Menu.
2. Disconnect both fanand
board interface connectors
from their respective
connections at the back of
thePrecellys® Evolution Touch.
5. Lift the lid o thePrecellys®
Evolution Touch.
8. Close the inner box.
4. Press OK and wait 60
seconds.
3. Select «Updatesoftware
version».
3. Unscrew the 2 screws on
the cover of thePrecellys®
Evolution Touch.
6. Replace the module on top
of the Precellys®lid and place
the entire unit inside the foam
protector and on the inner box
9. Place inside the main
cardboard box using the
handle
5. The Precellys® Evolution will
restartautomatically.
A REVOIR AVEC LES BONS ELEMENTS

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3.3. Storage
The device must be stored in a dry location at a temperature between
+0°C and +50°C on a horizontal surface.
The device may be stored after checking the following points:
1.The module is empty
2.The device is dry, clean and decontaminated
4. USE
4.1. Description of the user interface
4.1.1. Main interface
Note: for information about navigating the Precellys® Evolution from
the screen interface, please refer to the Precellys® Evolution user manual
(23405-800-DU002-A).
4.2 Starting temperature-controlled homogenization
4.2.1 Installing your Cryolys® Evolution
Use of the Cryolys® Evolution requires dry ice sticks.
Note: Wear personal protection equipment (PPE) as described in paragraphs
1.1.2 and 1.1.3.
1. Fill the cooling module with dry ice
Note: Fill the cooling module up to the level of the interior ns to optimize
the production of cool air.
2. Close the cooling module with the cover
3. Clip the cooling module to the Precellys® lid
4.2.2 Prepare the samples
1. Place the samples into the Precellys tubes and add the required buer
if necessary
Note: Bertin Instruments recommends the use of the Precellys® Lysing Kits.
2. Close the tubes and arrange them ad hoc in the tube holder
Note: Bertin Instruments guarantees the compatibility of all Precellys®
consumables and accessories with the Cryolys® Evolution
4.2.3 Homogenizing the samples
Select a saved program or use the «work» function (See the Precellys®
Evolution Touch user manual 23405-800-DU002-A)
Note: Saved protocols must include Cryolys® Evolution parameters. The
Cryolys® Evolution module’s activation eld must be set to ON.
The Cryolys® Evolution allows two operating modes, one automatic and
one manual.
Figure 4: Precellys® Evolution Touch main interface screen
A REVOIR AVEC LES BONS ELEMENTS
1 2
3
4
5
6
Mixer protocol settings interface: see Precellys® Evolution
Touch user manual 23405-800-DU002-A.
Cooling protocol settings’ interface: allows you to set the
variousparameters of the Cryolys® Evolution.
Note: the temperature display disappears and the Cryolys®
Evolution interface is grayed-out and deactivated.
Current temperature: indicates the temperature under the
Precellys® lid.
Cryolys® Evolution module activation eld (ON/OFF) : al-
lows you to activateor deactivate the Cryolys® Evolution
Operating temperature selection eld: allows you to set the
temperaturebetween 0 and 10°C
Operating mode selection eld: allows you to choose manual
or automaticmode
1
2
3
4
5
6
Do not inhale dry ice vapors: risk of asphyxia.
Ensure the dry ice source container is closed after
lling the Cryolys® Evolution.
Use the device in a ventilated area.
Do not open the Precellys® Evolution Touch lid or the
cooling module cover until the cycle completes.

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Manual Mode
Automated Mode
Tubes insertion
Tubes insertion
Tubes collection
Tubes collection
Pre-cooling
Pre-cooling
Homogenization
protocol
Homogenization
protocol
Target temperature maintained
Target temperature maintained
Figure 5: diagram showing automatic and manual modes
Program the homogenization parameters via the dedicated
interface: volume, speed, number & duration of cycle and pause
Program cooling parameters via the dedicated interface: ON/
OFF, temperature and mode
Note: You can use a saved protocol if the Cryolys® Evolution
module activation eld is set to ON.
Place the tubes and tube holder (tted with the plate) into the
Precellys® Evolution (refer to the Precellys® Evolution Touch
user manual 23405-800-DU002-A)
Close the Precellys® lid
Press the START button on the Precellys®
Evolution Touch
Note: When the chosen temperature is
reached, the following message appears:
«Temp. is reached.» Protocol starting». The
homogenization cycle starts.
1
3a
4a
5a
2
Automatic mode 4.2.4 End of cycle
If there are no more tests to carry out:
1.Empty the dry ice from the Cryolys® Evolution into a suitable container.
2.Clean the Cryolys® Evolution using a wipe (reservoir, reservoir air out-
let,interior of diuser module, etc.).
3.Turn the Cryolys® Evolution switch to OFF.
At the end of the cycle, open the Precellys® lid and retrieve the
tubes and the tube holder
Insert new samples and close the Precellys® lid
Note: Ensure that the volume of dry ice is sucient. If no more
homogenization is required, empty the cooling module.
Close the Precellys® lid
Press the START button on the Precellys® Evolution
When the chosen temperature is reached,
the following message appears: «Temp. is
reached.» Open the Precellys® lid
Place the tubes and the tube holder
(tted with the plate) into the Precellys®
Evolution (refer to the Precellys® Evolution Touch user manual
23405-800-DU002-A)
Close the Precellys® lid
Press the START button on the Precellys® Evolution
At the end of the cycle, open the Precellys® lid and retrieve the
tubes and the tube holder
Insert new samples and close the Precellys® lid
Note: Ensure that the volume of dry ice is sucient. If no more
homogenization is required, empty the cooling module
6a
3m
7a
4m
5m
6m
7m
8m
9m
10m
Manual mode
Avoid all direct contact between your skin and cold parts of
the Precellys® Evolution Touch and Cryolys® Evolution.
Do not heat up the dry ice using a hair dryer or similar in order
to accelerate evaporation.
Handle only with suitable gloves whenever the module
contains dry ice.
If using the Precellys® Evolution Touch without cooling, ensure
that the Cryolys® Evolution is tted to the diusion cover to
avoid any projectiles in the event of a leak from a tube.

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5. CLEANING AND DECONTAMINATION
5.1. Recommendations
The device must be stored in a dry location at a temperature between
+0°C and +50°C on a horizontal surface.
The exterior of the device can be cleaned using a sponge or cloth which
has been dampened with water or household alcohol.
5.2. Decontamination protocol
In the event of splashing, any parts that might have been contaminated by
infectious agents must be decontaminated using a suitable disinfectant.
The user alone is responsible for the choice of decontamination
procedure.
Any parts that might have been contaminated may, for example, be
cleaned using a wipe dipped in bleach diluted to 6° Cl.
Do not spray anything directly onto the device, particularly on
theelectrical connectors.
Clean the instrument when it is unplugged.
Do not use a sponge-scourer to avoid damage to the device.
Do not use an airborne decontamination process.
Do not use caustic soda crystals or acetone which will
irreparablydamage the equipment.
Immediately remove any surplus liquids using a dry cloth.
6. SUPPORT AND DOCUMENTATION
6.1. Frequently asked questions
Before transporting the device, you must:
1.Check that there is absolutely no dry ice inside the module
2.Ensure there is no humidity or, if necessary, dry any humid areas
If you have to transport or ship the Cryolys® Evolution, arrange and pack
it by following these steps:
Questions Answers
Can the Cryolys® Evolution fall o when I open the
cover of the Precellys® Evolution Touch?
We recommend separating the cooling module
containing dry ice from the cover when operating
the cover
Can I use any type of ice (ice cubes, blue ice, ice
gel, etc.) instead of dry ice?
The Cryolys® Evolution must absolutely be used
with dry ice sticks only
Is it possible to save a protocol’s cooling
parameters?
Just as for Precellys® Evolution protocols, you
can save a protocol using the Cryolys® Evolution.
Homogenization and cooling parameters will be
saved simultaneously.
What is the maximum/minimum quantity of dry
ice that I can place inside the Cryolys® Evolution?
We recommend a minimum of 0.5 kg of dry ice
in the module which appears as a uniform layer
across the bottom of the module.
The maximum is dened by your ability to close
the module cover, or around 1.5 kg of dry ice..

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6.2. Troubleshooting
If any of these faults persist, contact technical support (see 6.3 Support).
Problem encountered Possible cause(s) Action(s) to be performed
Module is badly positioned Reposition the module
on the Precellys® lid.
Bad connection
1.Check that the interfaceboard
connector isplugged in
2.Check the state of theinterface
board, its cableand connector.
Not enough dry ice Check that the dry ice covers at least
the center of the reservoir.
The cooling system is not receiving
any air
1.Check that the fanconnector is
plugged in
2.Check the state of the fan,its cable
and connector.
Diuser holes are blocked Wait for the interior of the module
to defrost.
Cut the power Disconnect the Precellys® Evolution
from the power supply
Message appears:
«No Cryolys® Evolution
has been detected»
Message
«Temp. not reached» or
«Temp. not maintained
during protocol» appears
6.3. Support
If, despite the information contained in this manual, you are still unable to
resolve your problem, please contact your distributor.
For the latest information about our services and support, please go to
our website http://www.bertin-technologies.com.
You can also contact the Bertin Instruments team via email at: sample-
[email protected]
6.4. Disposal
This equipment is subject to selective collection in accordance with the
European directive on waste electrical and electronic equipment (WEEE)
2012/19/EU.
Bertin Technologies is responsible for treatment of
WEEE for products sold in France (via RECYLUM).
For other countries, the importer is responsible for
treatment and this waste treatment is dependent on
current regulations.

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7. APPENDIX: EXAMPLE OF CE DECLARATION 7. APPENDIX: EXAMPLE OF CE DECLARATION

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NOTESNOTES

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