SCIEX CESI 8000 Plus Technical manual

CESI 8000 Plus High Performance
Separation-ESI Module
Site Planning Guide
October 2018RUO-IDV-05-3345-C | B11953AF

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEX
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USA
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1 Introduction..............................................................................................................................................5
Customer Site Planner Responsibilities..............................................................................................................................5
FSE Responsibilities............................................................................................................................................................6
During Installation.......................................................................................................................................................6
Customer Familiarization.............................................................................................................................................6
Technical Support..............................................................................................................................................................6
2 Site Planning Checklist.............................................................................................................................7
Customer Information........................................................................................................................................................7
Requirements.....................................................................................................................................................................7
Site Layout...................................................................................................................................................................7
Electrical Requirements................................................................................................................................................8
Ventilation and Waste Collection Requirements..........................................................................................................8
Environmental Requirements.......................................................................................................................................9
Computer, Network, and Software Requirements........................................................................................................9
Mass Spectrometer Requirements (CESI-MS Systems Only).........................................................................................9
Solutions and Equipment Requirements....................................................................................................................10
Customer Preparation................................................................................................................................................10
Mass Spectrometer Information.......................................................................................................................................11
Customer Profile..............................................................................................................................................................11
Comments and Exceptions...............................................................................................................................................12
Signoff.............................................................................................................................................................................12
A Site Requirements.................................................................................................................................13
Site Layout Requirements................................................................................................................................................13
Laboratory Layout and Clearances.............................................................................................................................13
Weights and Dimensions...........................................................................................................................................14
Electrical Requirements....................................................................................................................................................15
Mains Supply Connections.........................................................................................................................................15
Mains Supply Fluctuations.........................................................................................................................................15
Protective Earth Conductor........................................................................................................................................15
System Electrical Specifications..................................................................................................................................15
Ventilation and Waste Collection Requirements..............................................................................................................16
Environmental Requirements...........................................................................................................................................16
BioSafety Requirements.............................................................................................................................................17
Computer, Software, and Network Requirements............................................................................................................17
Controller Requirements............................................................................................................................................17
Printer Requirements.................................................................................................................................................17
LAN Connection (Optional)........................................................................................................................................17
Customer-Supplied Solutions and Equipment..................................................................................................................17
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Contents

Customer Preparation Requirements...............................................................................................................................18
Mass Spectrometer Requirements (CESI-MS Systems Only).............................................................................................18
SCIEX Mass Spectrometers........................................................................................................................................19
Thermo Scientific Mass Spectrometers.......................................................................................................................19
Bruker Mass Spectrometers.......................................................................................................................................20
Waters Mass Spectrometers.......................................................................................................................................20
B Equipment Safety Categories...............................................................................................................21
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Contents

This guide is for the site planner, the individual responsible for preparing the facility for the installation of the
system.
Note: Refer to the Safety Manual for instructions for safe use of the system.
Customer Site Planner Responsibilities
Complete the Site Planning Checklist on page 7 in consultation with facilities services personnel (electrical,
ventilation, and information technology [IT]), and return it to the SCIEX field service employee (FSE) before the
completion date. Refer to Signoff on page 12.
Note: The FSE will follow up if the checklist is not received prior to the scheduled installation date.
•Verify that adequate space and the required shipping or receiving facilities are available. Refer to Site Layout
Requirements on page 13.
•Provide all required electrical receptacles. Refer to Electrical Requirements on page 15.
•Verify that the requirements for ventilation and waste collection are met. Refer to Ventilation and Waste
Collection Requirements on page 8.
•Verify that the requirements for the operating environment are met. Refer to Environmental Requirements on
page 16.
•Verify that the requirements for the computer and network are met. Refer to Computer, Software, and Network
Requirements on page 17.
•Verify that all required solutions and laboratory supplies are available. Refer to Customer-Supplied Solutions
and Equipment on page 17.
•Verify that up to two operators have been identified for training. Refer to Customer Preparation Requirements
on page 18.
•For installations where the CESI 8000 Plus system will be used with a mass spectrometer:
•Verify the mass spectrometer to be used with the system is operational by executing a performance test.
•Verify that any required additional equipment for the ion source or mass spectrometer is present.
•Verify that someone who knows how to operate the mass spectrometer will be available during the
installation.
Refer to Mass Spectrometer Requirements (CESI-MS Systems Only) on page 18.
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1
Introduction

•When the shipment arrives, inspect the packaging exterior for damage. If there is any damage, then note any
issues on the delivery receipt and notify SCIEX immediately.
•Contact SCIEX Customer Service or the local FSE to schedule the installation.
FSE Responsibilities
Note: If the site preparation tasks are not complete when the SCIEX Field Service Employee (FSE) arrives, then
the scheduled installation will be postponed.
•Review the checklist and discuss outstanding issues with the site planner.
•Unpack and set up the CE equipment.
•Verify the performance of the system.
During Installation
WARNING! Lifting Hazard. Make sure that at least four people or a lifting device
are available to lift the CE system. Follow established safe lifting procedures. Refer
to the Site Planning Guide for the weights of system components.
The FSE unpacks the system (with the assistance of customer staff), sets up the system, and then confirms its
operation. When the system is installed, the FSE conducts an installation qualification.
Customer Familiarization
During installation, the FSE provides a system and software overview, reviews data, and provides some basic
operator familiarization.
Technical Support
SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughout
the world. They can answer questions about the system or any technical issues that might arise. For more
information, visit the SCIEX website at sciex.com.
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Introduction

Customer Information
Contact name
Organization
Address
City
ZIP code/Postal code
State/Province/Region
Country
Telephone
E-mail address
Requirements
Site Layout
Refer to Site Layout Requirements on page 13.
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2
Site Planning Checklist

N/ACompleteRequirements
There is adequate lab space to accommodate the equipment.
If the mass spectrometer inlet is lower or higher than the range of the CESI 8000
Plus cart, a table or stand of the appropriate height for the CESI 8000 Plus system
is available.
Additional personnel or lifting equipment are available to help the FSE move the
equipment. Four people are recommended.
Electrical Requirements
Refer to Electrical Requirements on page 15.
N/ACompleteRequirement
Installation of electrical supplies and fixtures complies with local regulations and
safety standards.
One branch circuit is provided for the CE components. One AC mains supply outlet
is required.
Note: Do not use extension cords.
The mains supply voltage does not fluctuate more than ± 10% from the nominal
voltage.
The mains supply includes a correctly installed protective earth conductor.
A qualified electrician has determined the appropriate mains supply configuration
based on the system electrical specifications. Refer to System Electrical
Specifications on page 15.
Ventilation and Waste Collection Requirements
Refer to Ventilation and Waste Collection Requirements on page 16.
N/ACompleteRequirement
Ventilation of the laboratory environment in which the system will be used
complies with local regulations and the air exchange rate is appropriate for the
work performed.
Waste containers are available as required by local regulations.
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Site Planning Checklist

Environmental Requirements
Refer to Environmental Requirements on page 16.
CompleteRequirement
The altitude does not exceed 2000 m (6562 ft) above sea level.
Temperature and humidity requirements have been met.
BioSafety Requirements
Refer to BioSafety Requirements on page 17.
N/ACompleteRequirement
The site is not designated as BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4).
Computer, Network, and Software Requirements
Refer to Computer, Software, and Network Requirements on page 17.
N/ACompleteRequirement
(Optional) A network printer or a dedicated printer and necessary print drivers
are available.
(Optional) An active, tested LAN connection is available.
Mass Spectrometer Requirements (CESI-MS Systems Only)
If the CESI 8000 Plus system will be used stand-alone, then select the check box and do not
complete the following table.
Refer to Mass Spectrometer Requirements (CESI-MS Systems Only) on page 18.
CompleteRequirement
A mass spectrometer will be available during the installation.
A performance test has been performed on the mass spectrometer and a printout of the results
is attached.
Any required equipment for the ion source or mass spectrometer is available at the site.
A person knowledgeable in operating the mass spectrometer will be available during the
installation.
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Site Planning Checklist

Solutions and Equipment Requirements
Refer to Customer-Supplied Solutions and Equipment on page 17.
N/ACompleteRequirement
All of the required solutions and bottles are available.
Customer Preparation
Refer to Customer Preparation Requirements on page 18.
N/ACompleteRequirement
Up to two customers have been identified for familiarization.
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Site Planning Checklist

Mass Spectrometer Information
If the CESI 8000 Plus system will be used stand-alone, then select the check box and do not
complete the following table.
Mass spectrometer manufacturer
Mass spectrometer model
Mass spectrometer serial number
Software version
Firmware version
Ion source model
Ion source serial number
Customer Profile
(Optional) To allow the FSE to provide site-specific familiarization, enter the following information.
Number of operators
Years of experience with mass spectrometry
Years of experience with capillary electrophoresis
Applications to be performed
Will the CESI 8000 Plus system be used stand-alone?
If the system will be used stand-alone, which detectors will be used?
UV
LIFPDA
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Site Planning Checklist

Comments and Exceptions
Signoff
Site planner contact name
I acknowledge that all of the installation requirements, as specified in this document, have been
met.
Completion date
(yyyy-mm-dd)
Site planner signature
Return date
(yyyy-mm-dd)
FSE name
FSE e-mail
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Site Planning Checklist

Site Layout Requirements
Return to checklist.
Laboratory Layout and Clearances
•The floor is smooth and level so that the mobile cart does not roll.
•The location allows at least 15.2 cm (6 inches) on all sides of the system.
•The location is away from heaters or cooling ducts, and not in direct sunlight.
•The location is away from vibrating equipment, such as refrigerators or centrifuges.
•For configurations where the CESI 8000 Plus controller is connected to the mass spectrometer acquisition
computer with a 4.3 m (14 feet) cable, the maximum distance between the controller and the acquisition
computer is 4 m (13 feet).
Figure A-1 Suggested Laboratory Layout
DescriptionItem
Distance to wall, minimum 15.2 cm (6 inches)1
CESI 8000 Plus cart with controller and CESI 8000 Plus system2
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A
Site Requirements

DescriptionItem
Distance to mass spectrometer approximately 10.2 cm (4 inches) (determined at installation)3
Mass spectrometer4
Height Requirements (CESI-MS Systems Only)
The alignment mark on the outside of the CESI 8000 Plus system is 36 cm (14 inches) from the bottom of the
system. This mark must be vertically aligned with the mass spectrometer inlet. To align the two systems, the height
of the cart provided with the CESI 8000 Plus system is adjustable.
The cart can be used with mass spectrometers with inlets that are located between 105 cm (41 inches) and 148
cm (58 inches) from the floor. If the height of the inlet is outside of this range, the customer must provide a table
or stand for the CESI 8000 Plus system.
Weights and Dimensions
Refer to the following table for weights and dimensions and make sure that the system can be moved to the
installation site. Make sure that the installation site can accommodate the equipment dimensions, weight, and
associated clearance.
WeightDepthWidthHeightEquipment
85.3 kg
(188 lbs)
72.4 cm
(28.5 inches)
63.5 cm
(25 inches)
Cover open: 99.1 cm (39
inches)
Cover closed: 73.1 cm (29
inches)
CESI 8000 Plus system
69.0 kg
(152 lbs)
73.7 cm
(29 inches)
91.4 cm
(36 inches)
66.6 cm to 111.8 cm
(27 inches to 44 inches)
Rolling cart for CESI 8000
Plus system
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Site Requirements

Electrical Requirements
Return to checklist.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the
installation of all of the electrical supplies and fixtures and make sure that
all of the installations adhere to local regulations and safety standards.
Provide a branch circuit with an AC mains supply outlet for the cart.
Do not use extension cords.
Mains Supply Connections
WARNING! Electrical Shock Hazard. Make sure that the system can be
disconnected from the mains supply outlet in an emergency. Do not block
the mains supply outlet.
Mains Supply Fluctuations
The CESI 8000 Plus system requires consistent voltage between 100 VAC to 240 VAC.
Protective Earth Conductor
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the
protective earth conductor. Any interruption of the protective earth conductor
creates an electrical shock hazard.
The mains supply must include a correctly installed protective earth conductor that must be installed or inspected
by a qualified electrician before the system is connected.
System Electrical Specifications
Note: Specifications are subject to change without notice.
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Site Requirements

Table A-1 CESI 8000 Plus High Performance Separation-ESI Module Electrical Specifications
ValueSpecification
Instrument: 100 VAC to 240 VAC, auto-ranging
Cart: 100 VAC to 240 VAC, auto-ranging
Controller: 100 VAC to 240 VAC
Monitor: 100 VAC to 240 VAC
Nominal input voltage
50 Hz or 60 HzFrequency
Instrument: 8 A
Cart: 4.5 A
Controller: 2.8 A
Monitor: 1.5 A
Maximum input current
Ventilation and Waste Collection Requirements
Return to checklist.
Ventilation Requirements
Refer to the "Laboratories" chapter in ASHRAE, 2011 Handbook - HVAC Applications for recommended
laboratory ventilation guidance.
Waste Collection Requirements
WARNING! Biohazard, Toxic Chemical Hazard. Follow local directives when
disposing of chemicals, vials and caps, and the remains of the prepared
samples, if applicable. They might contain regulated compounds and
biohazardous agents.
Environmental Requirements
Return to checklist.
•Altitude not exceeding 2000 m (6562 feet) above sea level
•An ambient temperature of 15 °C to 30 °C (59 °F to 86 °F)
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Site Requirements

•Relative humidity from 20% to 60%, non-condensing
BioSafety Requirements
The site must not be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). SCIEX does not install,
service, or repair SCIEX systems in areas designated BSL-3 or BSL-4.
Computer, Software, and Network Requirements
Return to checklist.
A computer (also referred to as a "controller") and monitor are supplied with the CESI 8000 Plus High Performance
Separation-ESI Module system.
Refer to the Site Planning Guide for the mass spectrometer for detailed requirements for the mass spectrometer
acquisition computer.
Controller Requirements
The controller and monitor are provided with the CE system.
CAUTION: Potential System Damage. Other than security software, do not install additional
software on the controller. Changes to the configured software could void the system
warranty and cause the system to stop operating. All operators require read/write access
to the C:\32Karat folder.
Printer Requirements
The system can be connected to a network or dedicated printer.
LAN Connection (Optional)
The controller can be connected to the network.
Customer-Supplied Solutions and Equipment
Return to checklist.
WARNING! Toxic Chemical Hazard. Refer to the chemical product Safety Data Sheets
and follow all of the recommended safety procedures when handling, storing, and
disposing of chemicals. For health and safety precautions, refer to the User Guide.
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Site Requirements

General Equipment
•Appropriate personal protective equipment (PPE)
•Lint-free wipes
•Pipettors (2 μL, 10 μL, 20 μL, 100 μL or 200 μL, and 1 mL) and appropriate tips
•pH meter
•Benchtop centrifuge
Glassware and Consumables
•Glass bottles (20 mL, 100 mL)
•20 mL glass vial
•0.5 mL centrifuge tube
•50 mL volumetric flask
Reagents
•Double-deionized (DDI) water (MS-grade water filtered through a 0.2 μm filter and with resistance above
18 M )
•1.0 N NaOH (Sigma PN 319511 or equivalent)
•0.1 N HCl (Sigma PN 84428 or equivalent)
•0.1 N NaOH (SCIEX PN 338424 or equivalent)
•7.5 M ammonium acetate (Sigma PN A2706)
•Glacial acetic acid (Sigma PN A6283 or equivalent)
•Methanol (Fisher PN A454 or equivalent)
Customer Preparation Requirements
Return to checklist.
After the system has been installed, an FSE will demonstrate basic system operation to up to two operators.
Operators should be available for the entire training period.
Mass Spectrometer Requirements (CESI-MS Systems
Only)
Return to checklist.
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Site Requirements

Before the Installation
Before the CESI 8000 Plus system can be installed, the mass spectrometer must be operational. To demonstrate
this, a performance test should be performed and a printout of the results attached to this document.
For some mass spectrometers, additional equipment is required, refer to the appropriate section below.
During the Installation
During the installation, a person who is familiar with the operation of the mass spectrometer should be available
to operate the system.
SCIEX Mass Spectrometers
For the OptiMS Adapter for SCIEX NanoSpray III source (PN B07363), all systems require the NanoSpray®III ion
source retrofit kit (PN 5030793), purchased from SCIEX.
Make sure that the required orifice plate for the NanoSpray®ion source is present (refer to the following table).
Orifice Plate
Part Number
NanoSpray®III Ion
Source Part Number
Model
10337315028129SCIEX Triple QuadTM 5500 system
QTRAP®5500 system
TripleTOF®4600, 5600, and 5600+ systems
10337315029154TripleTOF®6600 system
50189505028138SCIEX Triple QuadTM 6500 and 6500+ systems
QTRAP®6500 and 6500+ systems
Thermo Scientific Mass Spectrometers
For the OptiMS Adapter for Thermo Nanospray II MS Sources (PN B07366), make sure the additional required
equipment is present (refer to the following table).
Additional Required EquipmentIon SourceModel
Ion MAX adapterNanospray II ion sourceLCQ Deca XP MAX, LTQ series and the TSQ
series systems
Adapter ringNanospray II ion sourceLCQ Deca XP and LCQ Advantage XP systems
For the OptiMS Thermo MS Adapter for Nanospray Flex and Nanospray Flex NG Sources (PN B83386), no additional
equipment is required.
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Site Requirements

Bruker Mass Spectrometers
The OptiMS Bruker MS Adapter for Bruker Mass Spectrometers (PN B86099) requires the NanoElectrospray capillary
cap set (PN 212915), purchased from Bruker.
For Bruker mass spectrometers that are close to the ground, the CESI 8000 Plus system requires a low table or
stand. Refer to Height Requirements (CESI-MS Systems Only).
Waters Mass Spectrometers
No additional equipment from Waters is required for the OptiMS Waters MS Adapter for NanoLockSpray and
NanoFlow Ion Sources (PN B85211).
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Site Requirements