BestScope BS-6024RF User manual

Scientific Research Metallographic
Microscope
Model Number: BS-6024RF/TRF, 6025RF/TRF
Instruction Manual
BS-6024RF/TRF BS-6025RF/TRF
This manual is written for Scientific Research Metallographic Microscope of BS-6024RF/TRF, 6025RF/TRF. To ensure
the safety, obtain optimum performance and to familiarize yourself fully with the microscope, it’s strongly
recommended that you read this manual carefully before operating this microscope.

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Content
User Notice...............................................................................................................2
1. Components Name ...............................................................................................4
2. Structure...............................................................................................................5
3. Adjustment and Operation....................................................................................7
4. Installation ..........................................................................................................16
4-1 Installation diagram .......................................................................................16
4-2 Installation Steps............................................................................................17
5. Technical specifications.......................................................................................21
6. Trouble Shooting.................................................................................................24
6.1 Optical system................................................................................................24
6.2 Mechanical parts............................................................................................26
6.3 Electrical parts................................................................................................26

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User Notice
I. Safety Symbols
The following signs are on the microscope. Figure out what these symbols meanings and always use the
microscope in the safest way.
II. Safety Precautions
1. Be careful when moving the microscope, to avoid the lens glass sticked by fingerprints and sweat stains
for the sake of observation, to avoid the lenses dropped or damaged.
2. Do keep the microscope out of direct sunlight, high temperature or humidity, dusty and easy shaking
environment. Make sure the stage is smooth, horizontal and firm enough.
3. Hook into the upper slot behind the rack by one hand, and hold the front bottom of the rack by the other hand
when moving the microscope.
4. If the carrier, objective or viewing tube is spilled with bacteria or solution, you should immediately pull out the
power wire and dry the solution or water to ensure that the microscope is dry. Otherwise, it is possible to
damage the instrument.
5. Be sure to keep at least 10cm away from the left, right, top, rear of the microscope from walls and other objects,
so as not to plug natural convection air, which is used for cooling.
6. Make sure the instrument is earthed, to avoid lightning strike.
7. is used to ensure safety, you should make sure the main switch is in "O" (off) state and cut off the power
supply before replacing the bulb or the fuse. Check the input voltage: Be sure the input voltage which is signed
in the back of the microscope is consistent with the power supply voltage, or it will bring a serious damage to
the instrument.
8. Use the special wire provided by our company.
Symbol
Meaning
Main power switch is on
Main power switch is off
Ground connection
/
Upper / Lower lighting
Button press for upper lighting, button do not press for lower lighting
Trash bin
F.S-Diaphragm, --Aperture diaphragm opening direction, -Narrow direction
The switch of Light off while people leave
Photograph
Wrench used here
Light intensity direction, weaken to the left, strengthen to the right
CE certification passed

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III. Maintenance
1. All the lenses have been adjusted properly; Please do not dismount them by yourself.
2. The nosepiece, coarse and fine focusing parts are so delicate that it is forbidden to disassemble them carelessly
by yourself.
3. Keep the instrument clean, and do not pollute the optical elements when wiping away the dust on the instrument.
The objective lenses should be cleaned once every other month by professional.
4. We can clean the contaminations such as fingerprints and oil smudges on the prism by using the Lens paper and
solution, which is mixed by ether(70%) and alcohol(30%).
Noting that alcohol and ether are highly flammable, do keep them away from the fire or potential
sources of electrical sparks, and use them in a drafty room as possible as you can.
5. Do not attempt to use organic solvents to clean the microscope components other than the glass components.
Using neutral detergent to clean them.
6. You should cut off the power immediately and dry it if the microscope is wetted by the liquid when using the
microscope.
7. Do not try to disassemble any part so as not to affect functionality or lower the performance of the microscope.
8. The instrument should be kept in a cool, dry place and should be covered with a dust shield when not be used.
Cover the microscope after the lamp cooling down.
9. Working environmental requirements:
a) Indoor use; b) Temperature range:10℃~35℃;
c) Maximum relative humidity: when the temperature reached 31 ℃ is 80%, linear reduction of temperature
to 34℃ is 70%, when the temperature reached 37℃ is 60%, when the temperature reached 40℃ is 50%.
10. Storage and transportation requirements:
a) Temperature range: -40℃~+70℃ b) relative humidity range: 10%~100%
c) Atmospheric pressure range: 500hpa～1060hpa
warning:
Please operate the microscope in the manner specified in this manual, Otherwise, not only user's safety will be
endangered but also the microscope will be damaged. The microscope shall always be operated in accordance with
this instruction.
◎ This microscope will not cause radiation, electromagnetic interference and other phenomena to the
surrounding environment. It meets CE&UL certification standard.
The following symbols are used in this instruction to highlight text:
means failure to observe the warning in this instruction will result in personal injury to the
operator and/or damage to the instrument (including objects near the instrument)
★means failure to follow this instruction will cause instrument damage.
◎means annotation (for operation and maintenance)

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1. Components Name
The main difference between BS-6024RF/TRF and BS-6025RF/TRF is that BS-6024RF/TRF is manual microscope, BS-
6025RF/TRF has motorized nosepiece. BS-6024RF and BS-6025RF only have reflected illumination, BS-6024TRF and
BS-6025TRF have reflected and transmitted illumination, we will mainly introduce the operation of BS-6025TRF,
other models can refer to BS-6025TRF.
Eyepiece
Reflected Illuminator
Trinocular Viewing Head
Objective
Nosepiece
Stage
Condenser
Microscope base
BS-6025TRF

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2. Structure
1. Function shortcut keys
2. Filter switch lever
3. Coarse and fine lifting hand-wheel
4. Focusing upper position locking hand-wheel
5. Condenser height adjustment hand-wheel
6. Upper and lower lighting switch button
7. brightness control button
8. Long distance condenser
9. "ECO" infrared induction device
10. Objective switch key area
11. Stage movement hand wheel for X-direction
12. Stage movement hand wheel for Y-direction
13. Condenser diaphragm adjusting ring
14. Extended function board fastening screw
15. Extended function board
16. Excitation light label
17. Rotating spectroscope assembly room
18. Check partial slot
19. The partial slot
20. ND filter slots
* Manual version does not have this function

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BS-6024, 6025
1. Switching lever for optical path observation
2. Aperture switch button
3. Viewing diaphragm
4. Aperture diaphragm
5. Fastening screw for condenser
6. Condenser height adjustment hand-wheel
7. Photograph button
8. Field aperture size adjustment knob
9. Fine focusing hand wheel
10. Coarse focusing hand wheel
11. Lamp room fastening screw
12. Earthing screw
13. Lamp room socket
14. Lamp room plug
15. USB interface
16. Universal wrench

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3. Adjustment and Operation
Fig.1
Fig.2
3-1 Turn on the power (Fig.1)
Turn on the power, change the main switch on the
back of microscope (as shown by the Fig.1) into "-"
(power on) state.
3-2 Light intensity adjustment and upper & lower
light source switch (Fig.2)
As shown by the arrow in Fig.2, The light fades as the
button rotates. Instead, the light enhances.
As shown in Fig.2, when pushing the switch bar of up
and down light source, the upper light source is
lighting, the symbol is ; when pulling out
the switch bar of up and down light source, the
down light source is lighting, the symbol is
.
3-3 Light source socket (Fig.3)
As shown in Fig.3, there are red dots in the socket
and plug, the direction should be adjusted before
the plug is inserted into the socket, and red dots
should be inserted in alignment. When hearing
"click", it means ok.
Noting screen prints on the left and right socket
corresponds to switch levers of up and down light
source. As shown by the Fig: means light
socket of up light source, which corresponds to the
pushed state of switch bar for up and down light
source; means light socket of down light
source, which corresponds to the pulled state of
switch bar for up and down light source.
switch bar
of up and
down light
source
Dimmer hand-wheel
Red dot
Fig.3
When changing switch levers of up and down
light source, the statement of which is
different from the statement shown by the fig,
please check the plug and make sure the plug
is in the socket.

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3-4 Specimen preparation and placement (Fig.4, Fig5)
1. The specimen (Num② in Fig.4) for observation is fixed
on the sample plate (Num① in Fig.4) by use of silly putty
(Num③ in Fig.4), and ensure the specimen surface is
parallel to the sample plate. You can use the nipping
machine to press the sample. (as shown in Fig.5).
2. Place the sample plate of the fixed
specimen on the microscope stage.
Be careful when replacing the objectives, after the
specimen was observed with a short-range objective lens,
the objective may collide with the specimen when it is
need to replace the objective.
3-5 Pupillary distance adjustment (Fig.6)
The distance range between the pupils is 47mm～78mm.
When observing, we adjust the distance between the
pupils by holding the right and left prism eyepiece tube,
rotating around the axis until both left and right field of
view combined and feel comfortable.
Fig.6
Fig.4
①
②
③
Nipping
machine
Fig.5

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3-6 Three position light-path switch (Fig.7)
Select the desired light path and light intensity ratio as shown by Fig.7
Diagram
Push-pull rod Operation
Eye: Camera light ratio
Completely pushed
100: 0
Middle
20: 80
Completely pulled
0: 100
3-7 Focusing (Fig.8, Fig.9)
1. When no cameras are used:
Push-pull rod (as shown in Fig.7) is completely pushed. When binocular
observing, you can use 10× objective to focus. To prevent the specimen
from colliding with the objective lens, The mechanical stage should be
raised, which brings the specimen close to the objective, then slowly
reverse rotation of the coarse focusing wheel so that the specimen could
be down, meanwhile, you can search the image in the 10× eyepiece, finally
you can focus to clear with the fine focus hand-wheel. At that time even if
you convert to other multiplier objective, the specimen can reach the same
focus without any damage.
2. When Photographic devices are used:
Push-pull rod (as shown in Fig. 7) is completely pushed. Making binocular
observations, when the image is clear, then pulled it out, then it can be
observed by the camera system.
 The fine focusing hand wheels on both sides are held by magnets for easy
removal. All you need is the arrow shown in the Fig.9, Press one end of
the hand wheel inward to make the other curl, then gently pull off the
end of the cock, so hand-wheel is easily detached. Assembly also needs
only hand wheel center alignment put in. This function makes the two
fine-tuning hand wheels conveniently inter changeable, to avoid fine-
tuning hand-wheel and platform handle collision.
3-8 Adjust the tightness of the focusing hand-wheel (Fig.9)
The rotation direction is shown in the Fig.9, the more rotation, the tighter
the focusing hand-wheel is. On the contrary, the less rotation, the less tight
the focusing hand-wheel is.
Light-path
switch sign
Push-pull
rod
Fig.7
Fig.8
Fig.9
Tension adjustment ring

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3-9 Focusing upper limit (Fig.10)
⚫Actually, if you want to restrict locking of stage height (The upper limit of focus)
as appropriate, just turn the locking hand-wheel in the direction of the arrow (as
shown in Fig.10) at the corresponding upper limit position.
3-10 Electric condenser adjustment (Fig.11, 12)
⚫The center of the condenser should be coaxial with the optical axis of the
objective lens, the product has been adjusted before delivery, there is no need
to adjust the center again.
⚫The top position of the condenser, the product has been adjusted before
delivery, users don't need to adjust it by themselves.
⚫Turn the focusing hand-wheel of the condenser①, the condenser moves up and
down, make the aperture image of the field clear at each magnification.
Focus the lens in the middle position:
1. Turn the focusing hand wheel of the condenser①, Raise the condenser to the
highest position.
2. Focus the sample with 10x objective lens.
3. Rotate aperture ring②, narrow the field stop image to make it visible in the field
of view.
4. Turn the focusing hand wheel of the condenser①, focus the image of the field
aperture.
5. Move the field diaphragm to the center of the field with the universal adjusting
wrench turning the center of the field with the center screws ③ to the center
of the condenser.
6. Gradually open the field aperture, if the image of the field diaphragm is in the
center and in the field of view, which means the condenser has been correctly
aligned.
7. In practical application, slightly increase the aperture of the field of view to make
its image just cut out of the field of view.
8. Aperture diaphragm④ adjustment. Aperture diaphragm is designed for the
adjustment of numerical aperture, not for brightness control. Usually, When the
aperture diaphragm is opened to 70 to 80% of the exit pupil of the objective lens,
good image with enough contrast can be obtained, adjust the aperture
diaphragm with the scale mark on the condenser lens in accordance to objective
lens magnification.
9. Adjustment of aperture size in field of view.
During operation, rotate the aperture of the field diaphragm②, narrow the field
diaphragm and observe the field of view, if the aperture looks blurry, you can
turn the focusing hand wheel of condenser, lift condenser holder to make the
image clear, then turn the field diaphragm, make it just adjust to fill the eyepiece
field of view to reduce the noise of light and improve image quality.
②
Fig.10
Fig.11
Fig.12
locking
hand-wheel

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3-11 Use of extended feature boards (Fig.13)
As shown in Fig.13, a slot equipped with extended function
board is reserved on the nosepiece. When the microscope
lens is delivered, it is inserted into the slot with ordinary
light and dark field board. If you want to use other
extension function board, you just need to unscrew the
serial number ① and loosen it, pull out the standard light
and shade field board, and then insert the extension
function board into the slot and tighten the screw of serial
number①.
3-12 Computer control signal interface and camera
function key (Fig.14, 15)
When the external digital camera and microscope
computer control signal output interface (as shown in
Fig.15) supported by our company are all connected to the
computer, NOVA Basic software is running on the computer
at the same time. After capturing the real-time shooting
image of the camera, press the camera function button in
Fig.14 to take photos and record the images captured by
the current camera immediately (If you want to know the
detailed computer operation, please refer to related
software operation instructions)
Note: The camera function key is changed to ECO in the
manual version. ECO function: Power will be cut off
automatically after 15 minutes without operator's
presence.
camera
function
key
Computer control signal
interface
Fig.13
Fig.14
Fig.15
①

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3-13 Fuse replacement (Fig.16, 17)
As shown in Fig.16, firstly, open the drawer of the fuse box
with a universal adjustment wrench, then use a universal
wrench to gently push the fuse through the square hole
under the fuse drawer.
 The glass in the middle of the fuse is thin. When you open
the fuse box drawer and push out the fuse, please handle
it carefully and gently.
3-14 Key areas (manual version does not have this
function) (Fig.18)
As shown in Fig.18, the conversion process of objective lens
only requires the corresponding keys to enable the
microscope to perform the objective transformation
automatically. By default, the order from left to right is 5x,
10x, 20x, 50x, 100x, or the order of multiplier needs to be
confirmed by itself, which can be marked with the multiplier
tag attached randomly.
There are two modes of switching: free and directional.
Free mode: All the keys of the objective lens are valid, and
the key corresponding to the objective lens currently
observed is in a green light state.
Directional mode: You can only switch between the two
selected objective lenses, and the buttons of the other
objective lenses are invalid. The key corresponding to the
currently observed objective lens will be green light, while
the key corresponding to the to-be-switched objective lens
will be yellow light.
Preset method of two objective lenses in directional
switching mode:
Press the "function switch" button to keep it from loosening.
At this time, the indicator light of the objective lens switch
button is completely off. Then press the conversion button
corresponding to the two objective lenses you need to
direct, and the indicator light of the two objective keys you
press lights up. Finally, release the function switch.
Fig.16
Fig.17
Fig.18
Objective
Change key
Function
switch key
ECO function

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3-15 Additional function keys (manual version does not have this
function) (Fig. 19)
Figure 19 shows two function keys on each side of the microscope,
the functions corresponding to the function keys can be customized
on the computer through software. By default, the upper function
key is switched clockwise and the lower function key is switched
counterclockwise.
3-16 Use the ND filter rod (Fig.20)
As shown in figure 20, when the rod is pushed in, the filter is in use
state. When the rod is pushed out, the filter is in empty state. LBD is a
color temperature filter. ND25 is a color filter with a light
transmittance of 25%, and ND6 is a color filter with a light
transmittance of 6%.
3-17 Color filter use (Fig.21)
◎ Insert the filter rack most suitable for observation purposes into the
filter slot (Num①, Fig.21), and be sure to insert it from the left.
◎ The first sound "click" means the empty position, and the second, the
filter enters the light path.
Color filter
Applications
Color temperature
piece
Convert lighting to sunlight for general
observation and color photography
Green filter
Increase contrast for black-and-white
photography
Yellow filter
Increase contrast when semi-conductor wafer
is observed
Frosted sheet
Uniform light, but reduced light intensity
ND6
Adjust light intensity (transmittance: 6%)
ND25
Adjust light intensity (transmittance: 25%)
Fig.19
Fig.20
①
Fig.21
Upper function keys
Lower function keys
Right function
keys
Left function
keys
ND filter rod

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②
③
①
3-18 Reflective optical path choice (Fig.22)
Rotate the splitter chamber (Num.④, Fig.22) to the spectroscope
assembly room according to the requirements of the required
observation path.
BF1: Reflection field observations (transmittance: 6%)
BF2: Reflection light field observations
DF: Reflection light dark field observations
When rotating the spectroscope assembly, it must be complete.
* It is recommended to use "DF" for dark field observation. For light
field observation, please switch to "BF2", in case the light is too
bright to damage the eyes.
3-19 Diaphragm against mid-field view (Fig.23, 24)
1． Put the light brake lever on the "●" position.
2． Rotate the spectroscope assembly chamber (turntable) to the BF position.
3． Put the light brake lever on the " O " position and let the light go.
4． Rotate the objective to transfer the 10x objective into the light path, The
sample is then placed on the stage and the image is roughly focused.
5． Pull the lever ③ out of the field diaphragm on the lighting device until
the aperture diaphragm is at its minimum diameter.
6． Use a hexagonal wrench to insert the two field diaphragm into the
center screw②, adjust the image to the aperture to be located in the
center of the field of view (Fig.23 shows the adjustment process).
7． Push the pull rod③ along the aperture of the field and open the aperture
until the image of the aperture and the perimeter of the field of view are
inscribed. If the image is not correctly aligned, re-align until it is
centered.
8． Open the wide field of view diaphragm so that the image is exactly
connected to the field of view (c in Fig. 23).
● In the light field of reflected light
The field stop adjusts the lighting area to produce high-contrast images.
According to the objective lens used, Adjust the aperture knob of the field
(Num③, Fig22) of view of the reflecting illuminator until the aperture
image is outside the field of view, to screen out unnecessary light.
● In the light-dark field of reflected light
The diaphragm knob must be pushed forward to fully open the
diaphragm.
Fig.22
④
Fig.23
a
b
c

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3-20 Aperture diaphragm use (Fig.24, 25)
1． Move the light brake lever to the "●" position and close the light
path.
2． Rotate the spectroscope assembly chamber (turntable) to the BF
position.
3． Move the light brake lever to the " O " position and let the light go.
4． Turn the objective lens nosepiece so that the 10x objective lens enters
the light path, and then place the sample on the stage to roughly focus
the image.
5． Move the white board with the "cross" line until the "cross" is in the
middle of the field of view.
6． Turn the objective to transfer the empty position into the light path.
7． Push the aperture diaphragm out of the pull rod ③ until the aperture
diaphragm is at its minimum diameter.
8． Push the pull rod ② through the field diaphragm until it reaches the
minimum diameter of the field diaphragm. Meanwhile, the aperture
diaphragm image can be seen on the opposite central plate.
9． Insert the hex wrench into two aperture diaphragm alignment
screws④, the aperture image adjusted to aperture matches the
"cross" image.
●In the light field of reflected light
The aperture size is set between 70% and 80% of the numerical
aperture of the objective lens (As shown in Fig.25), this position is
generally the best viewing position.
●In the light-dark field of reflected light
You must push the aperture diaphragm lever (Num ③ in Fig. 24) to
open the aperture diaphragm.
◎For some samples, close the aperture diaphragm slightly, sometimes to
get both high contrast and low glare images. Therefore, it is
recommended that close the aperture diaphragm slightly.
3-21 Use the polarizer and analyzer (Fig.26)
1. The polarizer (Fig.27) with a screen print face forward, insert it into
the polarizer slot (Num.②Fig.26), and then move the polarizer into
the light path.
2. After the dust cover is removed, the analyzer (Fig.27) is pushed into
the slot (Num.① Fig.26).
3. Turn the polarizer rotor (Num.③ Fig.26) to find the darkest position
in the field of view.
Fig.24
③
Fig.25
Fig.26
②
①
③
Fig.27
Aperture Diaphragm Image
Analyzer
Polarizer

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4. Installation
4-1 Installation diagram
The following figure shows the installation sequence of each component, and the figure shows the installation
steps.
★Before installing, make sure all parts are free of dust and dirt. Do not scratch any part or glass surface.
★Keep the Allen wrench provided. You will also need it when changing parts.

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4-2 Installation Steps
Fig.1
Fig.2
4-2-1 Mechanical carrier stage bracket installation
(Fig.1, 2)
 Rotate the coarse focusing wheel so that the dovetail of
the frame rises until the limit screw exposure end face
as shown in the figure, then install the stage bracket
according to the path of serial number "C" in Fig.1. Align
the dovetail groove of the stage bracket with the
dovetail bulge of the rack in Fig.1, slide down naturally
until lower level screw is limited. Then the screw of
num1 in Fig.2 is screwed inward with a wrench.
The position adjustment of mechanical carrier stage
bracket can be adjusted by adjusting the position of limit
screw (As shown in the figure, the limit screw is installed
at B. When using the metallographic objective lens, the
limit screw can be installed at A).
4-2-2 Mechanical carrier stage equipment
installation (Fig.3)
 The mechanical carrier stage is installed along the path
of ramp in Fig.3. Firstly, the hole center of the stage is
roughly aligned with the center of the support circle,
and then the stage is placed on the positioning ring of
the support.
 After the stage is placed stable, tighten the screw of Ⅱ
with universal wrench in Fig.3, so that the stage can't
rotate and shake.
Fig.3
A
B
C

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4-2-3 Nosepiece installation (Fig.4)
Insert the slit into the dovetail groove of the microscope as
shown by Num③ in figure 5 and push it to the bottom, then
tighten the screw of Ⅲwith universal wrench to fasten.
4-2-4 Metallographic device installation (Fig.5)
The metallographic device shall be inserted into the head of
the microscope body in accordance with the serial number in
figure 5 and transferred to the appropriate position, then
tighten the screw with hexagon wrench to fasten.
4-2-5 Install observation head and C-mount of
photographic accessories 0.5X/1X (Fig.6)
1. Install the trinocular observation head.
The observation head is loaded into the circular dovetail of
the microscope according to the path (Num⑤) shown in
ordinal shape in figure 6, then tighten the screw of Ⅴwith
universal wrench to fasten observation head.
2. Install the interface of photo accessories 0.5x / 1x C-
mount (optional).
The interface of photographic attachment 0.5x / 1x C-
mount is loaded into the interface of the three-item
attachment of observation header according to the path
specified in the serial number ⑥ in figure 6, then tighten
the screw of Ⅵwith universal wrench to fasten photo
attachments.
 During the installation of the observation head, always
hold the observation head with one hand to prevent it
from falling and breaking.
 When the observation head is not in use and the
photographic accessories are not installed, please cover
the interface of the camera lens and the interface of the
accessory of the trinocular tube with the corresponding
dust cover to prevent dust intake.
Fig.4
④
Fig.5
Fig.6
Ⅳ
⑥
Ⅴ
Ⅵ
0.5x/1x C-mount
Trinocular tube
⑤

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4-2-6 Eyepiece installation (Fig.7)
Insert the eyepiece into the viewing eyepiece tube to the
side according to the path of Num ⑦ as shown by Fig.7.
4-2-7 Objective lenses installation (Fig.8)
1. Adjust the coarse focusing hand-wheel until the mechanical
carrier stage bracket is lowered to its lower limit.
2. The objective lens with the lowest magnification rate is
twisted to the nosepiece from the left or right side, then
Push the nosepiece clockwise, Install other objective
lenses in order from low to high magnification (as shown
in Fig.8).
 It becomes easy to change the magnification rate during
use by this objective installation method.
 Clean the objective glasses regularly, the objective lens is
very sensitive to dust.
 When operating, the samples were first searched and
focused using a 5x objective lens, then the objective lens
with other magnification was changed to observe
according to the needs.
 When rotating the objective lens, we can’t ensure that the
required lens enters the optical center until we hear the
"Clicking" voice.
4-2-8 Long distance condenser installation (Fig.9)
Adjust the small bracket to the lowest position, then screw
out the lower part of the condenser. The upper part is
inserted into the small bracket according to the path shown
in Figure 9, until the screw of serial number VIII is placed in
the slot, the upper part is pushed to the button, then screw
the lower and upper portion of the condenser, Finally the
bolt of Num Ⅶ is screwed according to the direction as
shown in Fig.9 to tighten the long-range condenser.
Fig.7