BestScope BS-7000B User manual

Inverted Fluorescent Microscope
BS-7000B
Instruction Manual
This manual is written for Inverted Fluorescence Microscope BS-7000B. For safety and keeping
the best performance, making you familiar with the instrument entirely, it is strongly
recommended that you read this manual carefully before using the microscope.

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User Notices................................................................................................................................ 2
1. Components Name................................................................................................................ 4
2. Installation................................................................................................................................5
2.1 Installing diagram.......................................................................................................... 5
2.2 Installment steps............................................................................................................6
3. Adjustment and operation....................................................................................................11
3.1 Lamp adjustment for fluorescence observation......................................................11
3.2 Reflected observing illumination adjustment.......................................................... 17
4. Microscope photography and video.................................................................................. 19
4.1 Microscope video........................................................................................................ 19
4.2 Microscope photography............................................................................................20
5. Outfit....................................................................................................................................... 21
5.1 Specification.................................................................................................................21
5.2 Objective Specifications............................................................................................. 22
6. Troubleshooting.................................................................................................................... 22

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User Notices
Safety Note
1. The epi-fluorescent attachment is a precise instrument. Open the box carefully, and avoid
dropping the accessories to ground and causing damage to them.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and vibrations.
3. Make sure that the lamp(mercury lamp) is installed correctly and all cords are connected firmly.
4. Do not open the lamp housing while it is turned on, lamp housing parts are extremely hot and
may cause burns if touched. Users can open the lamp housing at least 10 minutes after it has
been turned off.
5. Always be sure to ground (earth) the equipment.
6. Verify that the voltage and the frequency of the AC mains outlet match the setting of the voltage
switch and the frequency switch on the rear of the power supply unit.
7. Always use the power cord provided and make sure that the main switch is moved to “O”(OFF)
before connecting the power cord plug to the wall outlet.
8. To prevent any hazard, always turn the main switch on the power supply unit to “O” (OFF), unplug
the power cord plug from the mains outlet before replacing the lamp or the fuse, and wait for at
least 10 minutes before replacing the lamp. (Be sure to use a GCQ-100 mercury lamp.)
9. To prevent obstruction of the air flow, it is important to leave enough space around and above the
lamp housing.
Safety Symbol
Symbol
Meaning
The surface is very hot, not touch by your hands
Before using, please read the instruction carefully, improper operation will result in
bodily injure or instruction malfunction.
The main switch on
The main switch off

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Maintenance and Storage
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges,
wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%).
Since solvents such as ether and alcohol are highly flammable, they must be handled
carefully. Be sure to keep these chemicals away from open flames or potential sources of
electrical sparks. For example, electrical equipment that is being switched on or off. Also
remember to always use these chemicals only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the non-optical component of the equipment. To
clean these, use a lint-free, soft cloth lightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the power supply unit as malfunction or damage may occur.
4. In order not to impair the safety of the equipment, replace the lamp when the counter of NFP-1
indicates “100.00” hours. To prevent any hazard, always turn the main switch on the power
supply unit to “O” (OFF), unplug the power cord plug from the mains outlet, and wait for at least
10 minutes before replacing the lamp. High-pressure gas is sealed within the mercury lamp.
Thus, if it is continued to be used after its service life expectancy, the glass tube may deform and
may sometimes rupture.

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1. Components Name
Trinocular tube head Phase contrast slider Transmitted illumination
Reflected fluorescence units
Condenser
Illumination bracket
Eyepiec
Stage inserted plate
Stage
Body
Nosepiece
Objectives
。4×
。10×
。20×
。40×
Fluorescence power supplier
Protection barrier
B excitation, G excitation
Lamp house

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2. Installation
2.1 Installing diagram
The following Figure shows the installation sequence of the components. The number in the Figure
shows the installation steps.
*Before installing, be sure every components is clean, do not score any parts or glass surface.
*Keep well with the supplied hexagon wrench. When changing the components, you will need it again.
①
②
④
③
⑤
⑥
⑦
⑧
Condenser
Phase contrast slider
Transmitted Lamp house
Eyepiece
Trinocular head
Objectives
Glass stage
Power supply unit
Mechanical ruler
Microscope Body
Auxiliary Stage
⑨
⑩
⑾
⑿
Lever
Protection barrier
Mercury lamp
house

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2.2 Installment steps
2.2.1 Installing trinocular head(Fig 1)
Loosen the setscrew ③on the microscope body②
and insert the trinocular Viewing Head ①into the body
correctly, tight the setscrew ③.
2.2.2 Installing eyepiece(Fig 2)
Insert the eyepiece④into tube until they are against.
2.2.3 Installing condenser set(Fig3)
Install the condenser into the right direction(Fig3).
Fig 1
Fig 2
②
①
③
④
Fig 3

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Fig 4
A
2.2.4 Installing lamp house (Fig4, Fig5)
Insert the plug A on the lamp house into hole A of
power cord, then insert the plug B on the lamp house
into the B hole of the condenser till there are against.
(like Fig 6).
2.2.5 Installing phase contrast plate (Fig6,
Fig7)
1. Keep the slider face(the surface which has number
10-20) up towards. Each light ring or hole has its own
located position, so you need to move them until you
hear the “clicked” to ensure the ring or the hole reach
the center of the light path(show as Fig 7).
2. Turn the aperture diaphragm lever ①to adjust
aperture. Turn the diaphragm to a Max. aperture when
do phase contrast observation.
● The phase contrast ring has been centered
beforehand, so it needn’t to be adjusted when in the
use. If the ring is not in the center, you could adjust
by the centering bolt.
● The 10X/20X phase contrast ring is worked with the
10X, 20X phase contrast objective, while the opening
hole is used for bright field objective.
A
B
A
B
Fig 5
Fig 6
Notices
Environment Requirement:
1. Temperature: 0℃～40℃, max humidity: 85%.
2. High temperature and moisture will damage
instrument and affect performance.
3. Keep the instrument away from the dust environment,
and take the dust cover when no using.
4. Put the instrument on table without vibration.
Fig 7
①
Replace lamp
1. Turn the switch to off position when need to
replace the lamp. Pull out the lamp house and
then the lamp after it is cool down completely.
2. Insert the new lamp softly to prevent damage.
3. Please do not touch the lamp by bare hands, in
order not to reduce lamp working life or cause
explosion. Clean the fingerprint by wiping slightly
with soft cloth which is moistened with ethyl
alcohol.

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Fig 8
③
②
①
2.2.6 Installing the objective (Fig8, Fig9)
1. Turning the coarse focusing knob①like the figure shows
till the nosepiece get to its lowest position.
★For ensuring the safety of the instrument during
transportation, the nosepiece has been adjusted to
the lowest position and the tension adjustment
collar②has been adjusted to a appropriate tight
tension while leaving the factory.
2. Screw the lowest magnification objective on to the
nosepiece, then turn the nosepiece clockwise, mount
other objectives according the magnification sequence
from low to high.
◎Mount objective like this way will make the change of
magnification to be very easy in using.
◎The objectives also can be installed through the stage
opening.
★Clean the objective regularly, the objective used in the
inverted microscope is very sensitive to dust.
★Do cover all the unused holes on nosepiece with dust
caps③, to prevent the dust and contamination entering
inside.
★When operating, use the low magnification objective (4X
or 10X) to search and focus the specimen at first, then
use the higher magnification objectives if necessary.
★When change the objective, slowly turning the nosepiece
until you hear “clicked”, that means the objective enter
into the right position—center of the light path.
2.2.7 Mounting the Mercury lamp (Fig10, Fig 11)
1．Loosen the lamp socket clamping screw ①, and remove
the lamp socket. (Fig.10)
2．After removing the foam backstop②, securely insert
the + pole (the thick head) of the mercury lamp③to the
lower terminal first and then the – pole(the thin head) to
the upper terminal, then tighten the two clamping
screws④.
3．Close the lamp socket with lamp into the original position
and tighten the socket clamping screw①.
Fig 10
Fig 9
①

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Fig 13
●Be sure to use a 100W HBO ultrahigh pressure spherical
mercury lamp.
●Be sure to mount positive pole(the wide head) before the
other , or the damage to the lamp may occur.
●Never subject the lamp to excessive force when mounting the
Mercury lamp.
●Be careful and avoid leaving fingerprints or dirt on the mercury
lamp. Attached stain may cause distortion in glass which
could result in a ruptured lamp. If stained, wipe it a way gently
with clean gauze.
★To prevent any hazard, always turn the main switch on the
power supply unit to “O” (OFF), unplug the power cord from
the mains outlet, and wait for at least 10 minutes before
replacing the lamp.
2.2.8 Assembly of the Fluorescent Attachment ,
Cable and Cord Connections (Fig 12, 13)
1. Mount the lamp housing into the other end of the fluorescent
attachment and fix it with two screws④.
2．Plug the connector ⑤from the lamp housing securely into the
connector on the power supply unit and make sure the cord
is correctly connected. (Make sure that the main switch⑥of
the power supply is set to “O” (OFF) before connecting
cables)
3. Connect the power cord which along with the microscope into
connector on the power supply unit and connect the other
end of the cord to the mains jack.
● Verify the voltage and the frequency of the AC mains outlet
match the microscope pre-set voltage and frequency, which
is marked on the rear of the power supply units. Improper
input voltage and frequency may degrade lamp performance,
or in the worst case (although very rare), cause the lamp to
explode.
● It is better to use the power cord provided by us and the same
type power cord should be used if you lose or damage the old
one.
2.2.9 Fuse Replacement (Fig 12, 13)
1. Set the main switch to “O” (OFF) and unplug the power cord
before replacing fuses.
2. Using a flat-blade screwdriver, remove each of the fuses
holders⑦by tuning it anti-clockwise and pulling out.
3 Replace both fuses with new ones.
● Always use the designated fuses (2pcs 8A). Make sure the
voltage of the fuse match the voltage of the AC mains outlet.
② ③
Fig 12
Fig 11
④
⑥
⑤
⑦

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Fig 14
①
Fig 15
Fig 16
2.2.10 Installing the stage lengthen splint and
the mechanical ruler (Fig 14, 15)
◎Stage lengthen splint(auxiliary stage) can be
installed in either side of the stage to enlarge
the work surface. But you can’t install the
mechanical ruler together at same side.
◎Generally, the mechanical ruler will be installed
in the right side for comfortable adjustment.
1. Installing the stage lengthen splint
First, Screw the fixed bolt①on to the splint,
then mount it on to the stage from right or left
below, screwing down it until it stay firm.
2. Installing the mechanical ruler
Please install the ruler like the way of the stage
splint.
2.2.11 Installing protection barrier, glass
plate, lever (Fig 16)
1. Install the protection barrier on the condenser by
tightening the screw②.
2. Placing the glass plate to the right position.
3. Screw the lever③to the filter blocks box under
the nosepiece.
② ③

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3. Adjustment and operation
3.1 Lamp adjustment for fluorescence observation
3.1.1 Connecting power
Set the main switch of the power supply unit to “I” (ON). It will stabilize in 5 to 10 minutes after
ignition.
●Some mercury lamps may not ignite the first time the power is turned on due to variance in
production. If this occurs, set the main switch to “1” (ON), then press the TRIGGER button on the
front panel of the power supply no more than 4 seconds, repeat as necessary if the mercury lamp
still not on.
●To avoid shortening the lamp life, do not turn the lamp off within 15 minutes after ignition.
● The lamp cannot be re-ignited in 10mimutes after turning off, that is, until the mercury
vapor inside it has cooled down and become to liquid.
● Ensure that the hour counter is reset to “000.00” after replacement of the lamp. And you
can insert a thin object such as a mechanical pencil tip into the reset hole on the front panel
of the power supply unit to press the internal switch.
3.1.2 Function of button (Fig 1)
①Hour counter
②Ammeter
③Excitation button(TRIGGER)
④Reset button
⑤Power switch
3.1.3 Adjusting the diopter (Fig 2)
1. Look into the right ocular by your right eye,
then revolving the coarse focus knob to focus
on the specimen.
2. Then use your left eye to look into the left
ocular. If the image is not sharp, just use the
diopter adjustment ring①to adjust please.
★There are ±5 diopter in the adjustment
ring. The number which the reticle on the
eyepiece holder pointed is your eye’s diopter
graduation.
Fig 1
① ②
④ ⑤
③
on
Fig 2

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3.1.4 Adjusting the interpupillar distance (Fig3,
4)
When observing with two eyes, hold on the left
and right prism holder, turn around the axis, adjusting the
interpupillary distance until the left and right fields of view
coincide completely.
★The reticle on the interpupillary distance
indicator③,pointed by the spot “.” ②on the eyepiece
tube, shows the scale of the interpupillary distance.
(Fig.2)
The range of the interpupillary distance: 48～75mm.
3.1.5 Switching the light path (Fig 4)
◎Pulling out the light path selector lever④by your
finger, select the light path you needed.
◎when in the binocular observation, pushing in the lever
until you heard a “clicked”. While in video or
photography, pulling out the lever until it reached the
“clicked” position.
3.1.6 Mounting Auxiliary Stage (Fig 5)
1. When using mechanical ruler. Position the specimen
by moving the X,Y knob(120mmх78mm)
2. Use the standard specimen cover(1.2mm)for best
observation.
● Carefully replacing objectives. Or else the objective
may touch the inserted glass plate.
Fig 3
Fig 4
④
Fig 5
① ②

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Fig 7
② ①
Fig 8
⑤ ⑦
④
⑥
Fig 6
③
3.1.7 Centering the mercury lamp (Fig.6-8)
◎Before proceeding to center the mercury lamp, turn on
the power supplier, wait for the brightness of the lamp to
stabilize. When centering the mercury lamp, to protect
users’ eyes, please use the excitation light protective
shield (protection barrier).
1．Move the opening hole of nosepiece in to the light path.
Or remove one objective and turn the opening hole to
the light path.
2．Move the lever ①on the fluorescent unit, make the
green or blue excitation filter unit into the light path. If
U/V excitation filter unit is used, be sure to use the
protective shield.
3．Clockwise rotate the field diaphragm adjustment knob
②to open the diaphragm.
4．Place a piece of white paper on the stage and push the
filter slide ③into the light path, make the mercury lamp
filament projected on white paper.
5．If the mercury lamp filament is not projected onto white
paper, turn the condenser knob ⑤, make the mercury
lamp filament projected on the white paper. If the lamp
is not centered, adjust the filament knob④. (A)
6．Turn the lamp filament centering knob ④, adjust the left
and right halves of the filament to the center. (B)
7．Use the rear focus knob ⑥, adjust the mirror at rear of
the lamp housing, adjust the left and right to same
focus. (C)
8．Turn the filament centering knob ④to make the
filament image and mirror image overlap. (D)
◎Turn the collector adjusting knob⑤to make the field of
view as best as possible.
◎Maintain this condition until the next time the lamp is
replaced.

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Note:
● When the hour counter indicates “100.0”, set the main switch to “o” (OFF) for safety, wait for more than
10 minutes, then replace the mercury lamp after making sure that the lamp housing has cooled down.
A mercury lamp seals high-pressure gas inside. If the lamp is used beyond its service life, stress may
accumulate inside the lamp, and in the worst (but very rare) case, the lamp could explode.
● After replacing with a new lamp, reset the hour counter, be sure to press the reset switch until “000.00”
is displayed.

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Centering the mirror reflected image (Fig.7)
★The mirror reflected image has been centered before
leaving the factory. Please do not adjust the knob⑦
if not necessary. Only when the lamp has been
centered precisely, can the knob⑦be adjusted.
Note: once the knob is adjusted, the reflected mirror
cannot be reconverted to the status when leaving the
factory.
Knob control: (Fig.7):
1．The middle knob⑥is the mirror reflected image
focusing knob which can sharpen the reflected image,
it can adjust the mirror front and back.
2．The knobs at both sides⑦can adjust the up/down or
left/right position of the mirror reflected image.
3.1.8 Centering the Field Iris Diaphragm (Fig 9, 10)
1. Move the 10× objective in the light path, and place the
specimen on the stage and focus the microscope to
get a approximate image.
2. Adjust the the field iris diaphragm lever②
anti-clockwise until the diaphragm comes into the
smallest.
3. Use the hexangular wrench to adjust the two field iris
diaphragm centering screws③alternately to move the
image of the diaphragm to the center. (a of Fig.10
shows the adjustment of diaphragm)
4. After adjusting the aperture, clockwise rotate the field
diaphragm adjustment lever ②, open to biggest
aperture, can more clearly determine whether the
aperture is centered (b of Fig.10).
5. Enlarge the diaphragm until it just circumscribes the
field of view (c of Fig.10).
Fig 9
②
① ③ ④
Adjusting the field iris diaphragm
The field diaphragm adjusts the diameter of the illuminating
beam to obtain good image contrast.
Keeping the field diaphragm stopped down to the smallest
required area for each observation makes it possible to
prevent color fading of areas outside the observation target
region.
According to the objective in use, adjust the diaphragm image
using the field diaphragm lever so that the field of view is
circumscribed by the field diaphragm to exclude stray light.
Fig 10
c
b
a

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● To recognize the aperture diaphragm, you could remove the eyepiece if necessary (You also could
insert in the center telescope), then looked into the viewing tube, you might see a field of view like the Fig.
12 shows. The proportion could be changed by dialing the aperture adjustment lever according your need.
(①is the image of the aperture diaphragm, ②is the edge of the objective)
● Generally, when observing the colored specimen, you need to set the size of the condenser aperture
diaphragm at 70% ～80% of the numerical aperture which marked in the objective, but if observing the
bacterium specimen which not colored, you could turn the aperture diaphragm lever at the direction of
“ ”(clockwise).
3.1.9 Using color filters (Fig. 11)
◎Select the appropriate color filters according your
need, it became more effective to observe or
photography the specimen. Especially, we
suggest using the LBD color filter, which can
compensate more neutral colors.
◎You could pile up a group of color filters to the filter
holder, if you ensure they are level and the whole
thickness is less than 11mm.
Color filter
Meaning
IF550
Single contrast color filter (green)
(used for the phase contrast microscopy)
LBD
Color temperature transit color filter (blue)
(used for bright field observation and micro
photography)
3.1.10 Using the aperture diaphragm (Fig 12)
◎When in the bright field observation, the aperture
diaphragm control the numerical aperture of the
illumination system. Only when the numerical
aperture of the objective and the illumination
system are matching, you can obtain the higher
image resolution and contrast, and the increased
depth of field, too.
Fig 11
Fig 12
①
②
70-80%
30-20%

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3.2 Transmitted illumination adjustment
spanner
3.2.1 Turn on power, adjusting brightness(Fig 13)
Connect the power, turn on the main switch①(shown on
the Fig.13) which on the bottom side of the base to
“－”(on). Turning the brightness adjustment knob
clockwise②, the voltage raise, and the brightness
strengthen; whereas turning at the contrary direction, the
voltage decline, and the brightness weaken.
◎Using the lamp in a low voltage condition, will prolong the
service life.
3.2.2 Adjusting the Tension Adjustment Collar
(Fig14)
The tension of the coarse focusing knob ②
has already been adjusted properly before
leaving factory.
◎How to adjust tension of the coarse focusing knob?
Turn the tension adjustment collar ①with the plastic
spanner. While revolving at the direction of the arrow in the
Fig. 14, the tension of the coarse focusing knob②is
increasing, and if at the contrary direction, the tension will
decline.
3.2.3 The centering ring (Fig 15, 16)
★Usually you do not need the operation of centering.
If necessary, please follow the following steps:
1. Place the specimen on the stage and focus it.
2. Take out the eyepiece, replace it with the CT ( the
centering telescope), and inserted it into the viewing
tube without diopter adjustment.
3. Make sure the matched phase contrast objective and
phase light ring (in the phase contrast slider) have
been in the center of the light path.
Fig 13
②
①
Fig 14
③
②
①
Fig 15
③
Fig 16

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4. Using the CT to look the light ring’s image①and the phase contrast ring’s image②, if the light ring’s
image is not sharp, please shifting the CT’s ocular until you can see a clear image of the light ring②.
5. Adjusting the bolts of the two centering holes③in the phase contrast slider by the screwdriver ③until
the light ring center and the phase contrast center are coincided.
6. The 10X and the 20X phase contrast objective use the same light ring on the phase contrast slider.
So you need to check the coincidence of the light ring center and the phase contrast center when
changing the objective. If having departure, you ought to center again.
★If the light ring is centering incorrectly, you will fail to obtain the best viewing effect of the
microscopy.
★After removing or replacing a thick specimen, the light ring and the phase contrast ring are
likely to deviate each other, which will result in a decline of the image contrast. So if happened,
please repeat the steps as above.
★If the container or the cover flip which used to place the specimen is not flat, it maybe need to
repeat the centering steps for obtaining a more contrast effect. Please center the light ring by
the phase contrast objective, according to the sequence of low to high magnification.

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4. Microscope photography and video
4.1 Microscope video
Fig 1
4.1.1 Selecting the light path (Fig.1)
★just used in the trinocular observation
1. Pulling out the light path selector lever, until you
heard the “clicked”.
★In the dark specimen observation, you can make the
focus by both eyes at first, then change the light path.
4.1.2 Installing the video set (Fig.2)
1. Loosen the locking bolt①on the trinocular viewing
tube, and take out the dust cap②.
2. Remove the dust cover on the both ends of the video
accessories③, and revolve the screw head end into
the CCD/CMOS port.
3. Install the accessories into the tri-through port, and
screw down the bolt①.
4.1.3 Focus (Fig.2)
Doing a binocular observation at 20% brightness,
look the image on the video or the computer which
connected with the microscope video system when
the image is sharp. If it is not in focus, please turning
the revolving video connected tube④until the image
is sharp enough.
Fig 2
Video