BestScope BS-5070B User manual

BS-5070B/T
BS-5070BTR/TTR
Polarizing Microscope
User Manual
To obtain optimum performance and to familiarize yourself fully with the use of your
microscope, we recommend that you read this manual thoroughly before use. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
BS-5070B/T do not have reflected illumination system, for other parts, they are the same
as BS-5070BRT/TTR, the operation is also similar.

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Safety notice ……………………………………………………………1
1.Structure and nomenclature……………………………………………4
2. Technical parameter and specification …………………………………7
3. Configuration …………………………………………………………9
4.Assemblage …………………………………………………………10
5. Operation ……………………………………………………………13
6. Maintenance…………………………………………………………19
7. Troubleshooting………………………………………………………20

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Safety notice
1. Transportation
As microscope is a precision instrument, handle with care,
avoiding impact or abrupt movement during transportation.
Do not push or pull the microscope during using, otherwise
the precision for image will be reduced.
1.1 Hold the curve and keep the microscope in balance
1.2 Do not hold the focusing knob, eyepiece tubes and stage
as these parts are movable. Troubles maybe caused by such
handles.
Do not make specimen or filters fall off.
2
.
Working Environment
2.1 Temperature range is 0℃～40℃and the max. humidity is 85%
2.2 Altitude :<2000m
2.3 Barometric：86KPa～106KPa
2.4 Avoid high temperature and humidification otherwise there will be fog or mold on
the lens
2.5 Keep the environment breezy and cover the microscope with the dust-cover
2.6 Avoid violent vibration as the vibration will reduce the image’s quality
3. Focusing knob
Never turn the left and right focusing knob in the
adverse direction at the same time. Do not turn the
coarse focusing knob when the stage reaches max.
position. These improper operations will make
focusing structure unworkable.
4. Clamp
The clamps fix the microscope firmly during transportation.
Please remove them before using.
5. Do not take the microscope apart
as it will be damaged. Such operations might have bad effect on the performance and
make user get an electric shock or injured. Please contact with manufacturers if there
is any problem.
Never turn the left and right focusing knob in
the adverse direction at the same time
curve
clamp

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6. Use the space parts supplied by the manufactures
The bulb, fuse and electronic cord have been assembled already in the factory and
please make sure to use the spare parts of them supplied by the manufacturers.
Improper bulb, fuse and electronic cord will destroy microscope and cause fire. Please
make sure to use PE electronic cord when using extra-long electronic cord.
Spare bulbs: halogen bulb 12V 20W, 12V50W（reflect）
LED lamp 5W(transmission), 10W（reflect）
Spare fuse: 250V 1A delayed model, 5 × 20 minitype fuse
7. Mounting the bulb
Never touch the surface of bulb with your hand directly. Please use gloves or cloth
material when you mount the bulb to avoid leaving fingerprints. Fingerprints or stains
should be wiped off with a tissue moistened with a small amount of alcohol. The
fingerprints will etch on the bulb’s surface and make brightness lower and life cycle
shorter.
Please make sure the bulb’s touching points are Ok as it will not light or short circuit
if the bulb’s touching points are damaged. Insert the bulb’s legs into the bulb holder
firmly. The bulb will go out if its legs have not been inserted into the holder firmly.
Such Operation may cause short circuit or smoking. At last, please make sure if the
collector has been mounted properly
8. Temperature for illumination
There will be high temperature when the bulb is lightened, never move the collector
when the bulb is lightened. Do not touch the bulb within 30 minutes after it has been
gout out. Make sure the bulb has been be cooling enough time (at least 30 minutes) to
avoid scald.
Do not put fibre, papers and incendive things (eg. Gasoline, aether, methanol and
ethanol) close to the bulb.
9.Make sure to turn off the power supply before assembling microscope,
replacing bulb or fuse.
Notice:
The instruction you buy may not including some products mentioned in this
instruction. Safety has been considered during design while users still have the
possibility to be injured and the instruction to be damaged when improper
operation made. Please pay much more attention to read this instruction
carefully before using and keep it properly to make sure it will be got when it is
necessary.

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Mark Meaning
1.Hot surface, don_t touch with your hands
2.Read the instruction manual carefully before operating the micuscop
unproper
operation will cause hurt or dameger to the operator and instrumen
3.Switch on
O 4.Switch off

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1. Structure and nomenclature
Eve fubes fixture
Bertrand lens
focusing plate
Bertrand lens unit
Transmission/reflect
Conversion switch
Microscope body
Bertrand lens centering
screw
condenser
Revolving round stage
Li
g
htness ad
j
ustin
g
Objective
Nose
p
iece
Bertrand lens unit
clam
p
in
g
knob
Analyzer
Bertrand lens unit
Binocular head
Cross Eyepiece
Eyepiece
Com
p
ensator
Reflected illumination
system
Aperture stop

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Coarse focusing knob
moment adjusting ring screw
Fine focusing
knobscrew
Consender movement
knobscrew
Fine focusing
Knob screw
Coarse focusing
knob screw
Condenser
centering screw
Objective centering
screw
The top lens of
the condenser
Polarizer knob screw
Clamping knob screw

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Lamp house
Knob screw
Mirror selector lever
Polarizer Field stop
knob
Aperture stop
knob
Field stop
centering screw
Aperture stop
centering screw
Color filter
Lamp house
Power plug Power switch

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2. Technical parameter and specification
2.1 Optical system: infinity optical system
2.2 Objectives: stress-relieving infinity plan objectives
Magnification NA Cover glass thickness(mm)
4X 0.10 0.17
10X 0.25 0.17
20X 0.40 0.17
40X 0.65 0.17
60X 0.80 0.17
100X (OIL) 1.25 0.17
20X 0.4 0
40X 0.65 0
60X 0.8 0
100X 0.9 0
2.3 Eyepiece:
Category Magnification Field of view（mm）
Eyepiece: 10X 20
Crisscross eyepiece 10X 20
Eyepiece with scale of
crosshair
10X 20
eyepiece with gridding 10X 20
2.4 Total magnification
objective 4X 10X 20X 40X 60X 100X
eyepiece 10X
Total
magnification 40X 100X 200X 400X 600X 1000X
2.5 Nosepiece

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Centerable quintuple
2.6 Rotatable stage：
Diameterф160mm，360°scale。Minimum reading 6’by means of vernier scale
2.7 Coaxial Coarse and Fine Focusing System,
Sensitivity and Graduation of Fine Focus: 0.002mm
Focusing movement range：24mm
2.8 Illumination system:
Transmission light：Halogen lamp 12V20W
Reflected light：Halogen lamp 12V50W
2.9 Eye tubes:
Trinocular tube
2.10 Filter:
Blue, green, frosted,gray
2.11 Test plate compensator
λtest plate（first-order red）,1/4λtest plate, Quartz wedge test plate
2.12 condenser：NA=0.9

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Objective
For transmitted light For reflected light
4X
10X
20X 20X
40X 40X
60X 60X
100X 100X
3. BS-5070BTR/TTR Configuration
10X eyepiece with
scale of crosshair
camera
10X eyepiece
mesh
10X
e
y
e
p
iece
10Xcrisscross eyepiece
λ—λ/4 test plate
Quartz wedge test plate
Hexagen ring spanner
2.5mm、2mm
Centering spanner Reflected illumination system

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4. Assembly Diagram
4.1 Removing the clamps for transportation
The clamps fix the stage and focusing adjustment
firmly during transportation.
Handle the grooves beside collector and pull it out.
Remove the clamps by wrenches.
4.2 Reflected illumination system
Mount the Illumination System①into the round dovetail on the head of the
microscope body properly and use the Allen screw driver to tighten the set screw②to
fix it.
Fig 1
4.3Mounting Bertrand lens unit (Fig.1)
Mount the Bertrand lens unit③into the round dovetail on the reflection Illumination
system ①properly and use the Allen screw driver to tighten the set screw④to fix
it.
4.4Mounting trinocular viewing head (Fig.2)
Mount the trinocular viewing head⑤onto the illumination system properly and use
the Allen screw driver to tighten the bolt⑥）to fix it.
③
②
①
④
⑤
⑥
⑤

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⑦
4.5 Eyepiece
The eyepiece is inserted into the left eye lens barrel, another cross eyepiece
(positioning) alignment righteyepiece tube within the gap insertion, the installation
should be pushed down to ensure thatthe adjustable eyepiece, eye baseline cross
divisional position unchanged.
During operation, such as the need to rotate 360
degrees when the eyepiece,ake off the binocular
plastic ring⑦, then insert into the eyepiece.
4.6 Objective
Adjusting the coarse focus knob until the support device of the mechanical stage reach
its low limit position.
Insert the 10X objective into the primary hole (which without centering holes on its
sides) on the nosepiece, then push the nosepiece clockwise and install other objectives
by the sequence of low to high magnification.
4.7 Condenser
Before delivery, the condensing lens is mounted on the microscope. According to the
following steps to remove or alter the condenser:
1) The stage of adjustment to the highest position,
rotating mirror elevating handwheel will mirror
bracket falling to the appropriate position.
2) the first side of the condensing lens center
adjusting screws, can be taken out condenser
when installing the condenser, in stent, tighten
the screws.
4.8 Mounting Test Plate Compensator
Insert the optical compensator into the test plate adapter.
4.9 Filter
Transmission light：The filter has been mounted in the microscope before shipment.
By in case of need, it can be taken out the filter from the bottom of condenser.
Reflected light： Engage the optimum filter sliders for the purpose of observation in the
filter insertion slots.
4.10 Mounting Polarizer
Reflected light: Insert the polarizer into its slot and make it in the light path center.
NOTE: the polarizer and analyzer are engaged when pushed in to the second
click-stop position. To disengaged the polarizer and analyzer, and to place the empty
hole in the light path, pull the analyzer out to the first click-stop.

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Analyzer
Halogen lamp
Micrometer
Objective
λ-λ/4 test plate
Tissue slice pedestal
Polarizer
Mesh part Crisscross
part
Color filter
Polarizing
ruler
Hexagen ring
spanner
Centering
spanner
Filter
Eyepiece
Objective
Objective
Eyepiece
Eyepiece
Quartz wedge test plate
Color filter
The silkscreen
“- -”means polarization direction is north and south.
“• • ”means polarization direction is west and east.
4.11 Polarizing ruler
By in case of need, install the polarizing ruler on the revolving round stage.
Note: parts when not in use can be shown in the aluminum alloy box save
5. Operation
5.1 Illumination
Connect the power; press the main switch that on the backside of microscope body to
the “I” (on) position.
When using transmitted light illumination, press the Transmission/reflect conversion
switch to the “I” (on) position.
When using reflected light illumination, press the Transmission/reflect conversion

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switch to the “II” (on) position.
Turning the brightness adjustment knob clockwise, the voltage raise, and the
brightness strengthen; turning with the anti-direction, the voltage decline, and the
brightness weaken.
5.2 Interpupillary range
Adjust the siendentipf binocular to make sure that view field in the right and left
eyepiece is consistent. The point “·” stands for the interpupillary distance
5.3 Focus
Use the 10×objective to focus. To avoid the objective touch with the specimen, you
should raise the mechanical stage and let the specimen close to the objective, then
slowly separating them to focus. The operator can converse turn the coarse focus knob
to get the specimen down ,and search images in the 10×ocular simultaneously, then
use the fine knob to focus. At this moment, you can replace other magnification
objectives safely, and focus without the risk of destroying the specimen.
The tension of the coarse focusing knob has been adjusted before leaving factory. If it
is too loose, for example, the stage decline slightly due to self-weight, the tension
adjustment collar can be adjusted.
(When focus on the specimen by 40X or 60X objective, make sure the top lens of the
condenser is in the light path center.)
NOTE:
Slide the mirror selector lever toward the indication of the mirror for the desired

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observation method.
T: Transmission light observation
R: Reflected light observation
5.4Adjusting the optical axis
The revolving nosepiece is centerable. Before leaving the factory, the optical axis of
the objective and the center of stage has been adjusted coaxial, please do not adjust
them unless necessary.
★Make sure the 10X objective is inserted into the primary hole on the centerable
revolving nosepiece.
Perform the following adjustments if the optical axial and the center of stage is not
coaxial.
1) Focus on the specimen by 10x objective and look for an easily recognizable detail
①in the field. Move this detail in the center of the eyepiece cross lines.
2) When the stage is rotated, the detail moves in circle ②. Manipulate the two stage
centering knobs to coincide the imaginary center of the circle circumscribed by the
detail with the intersection of the eyepiece cross lines.
3) Moving the specimen only, move a new specimen
detail into the cross line center.
3) Repeat steps 2 and 3 several times until the
center of the stage rotation is in the center of
the cross lines, i.e., when rotating the stage,
the specimen stays in the center of the cross lines.
This concludes centering of the optical axis for the 10X objective, which will act as
the reference objective. Now, center the other objectives with the centerable nosepiece
by engaging the objectives into the light path one after another. (Each objective has 2
centering screw on the nosepiece.)
5.5 Orthoscopic Observation
Revolve the Bertrand lens dial to “O” position to remove the Bertrand lens from
optical path. Push the analyzer into its slot. The polarizer and the analyzer can be
①
②

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360°rotatable, turn anyone until complete extinction is obtained.
Place the specimen for orthoscopic observation.
Insert test plates for further observation test and study.
5.6 Adjusting the aperture stop axis
When using transmitted light illumination, revolve the Bertrand lens dial to “B”
position or remove the eyepiece, observe for the back focal plane round bright of
objective lens, slowly open contraction the aperture diaphragm, diaphragm and
observation of bright circle concentricity, such as offset adjustable condenser center
adjusting screw and achieve both coincidence.
5.7 Adjusting the diaphragm
The center of the aperture and field diaphragm in the reflective lighting part, have
been adjusted, before products leave factory, in general do not open. When using
transmitted light illumination, if the aperture is not in the center of view field,
adjustable aperture adjusting screws.
5.7.1 Centering the Field Iris Diaphragm (FS)
1) Slide the mirror selector lever to “R”.
2) Engage the 10X objective by rotating the revolving nosepiece, place the specimen
on the stage and adjust approximate focusing.
3) Pull out the FS knob on the reflected light illuminator to reduce the aperture iris
diaphragm a little.
4) Rotate the two FS centering screws using the Allen screwdriver to adjust so that the
field iris image becomes concentric with the field of view.
5) While pushing in the FS knob, open the field iris diaphragm until the field iris
image inscribes the field of view. If the image is found to be eccentric, adjust the
centering again.
6) Open the field iris so that its image is almost the same size as (Fig. 28 subscribes)
the field of view.

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5.7.2 Centering the Aperture Iris Diaphragm (AS)
1) Slide the mirror selector lever to “R”.
2) Engage the 10X objective by rotating the revolving nosepiece, place the specimen
on the stage and adjust approximate focusing.
3) Remove the eyepiece, look into the eyepiece sleeve and pull the AS knob, so that
the aperture is about 70%.
4) If the center of the iris diaphragm is deviated, center it by rotating the two AS
centering screws using the Allen screw driver.
With some specimens, an image with high contrast and little flare may sometimes be
obtained when the aperture is slightly closed. It is therefore recommended to also try
a slightly closed aperture.
5.8 Compensator
1) gypsum plate (λ)
In the orthogonal polarizing filter has a purple interference color.The gypsum plate is
inserted into the optical path, can make mineral film interference color is raised or
lowered a grade.For example, a mineral thin-section interference color is a gray, is
inserted into the gypsum plate, if the same radius in parallel, the interference color by
a gray into level two blue green; if the synonym radius parallel, the interference color
on a gray to level orange.In general, gypsum plate compared to the interference color
is low (level two yellow below) mineral tablets.
2) mica plate (λ/4)
In the orthogonal polarizing filter has a gray interference color.Insert the mica plate,
can make mineral tablets by GC interference colors are listed in order of ascending,
Field iris dia
p
hra
g
mima
g
e
Fieldofviewofe
y
e
p
iece The ima
g
eofa
p
erture iris dia
p
hra
gm

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descending a color sequence.For example, a mineral flakes interference color is a
purple, join the mica plate, if the radius is parallel, the interference color is elevated a
color sequence and change to a two LAN; if the synonym radius parallel, the
interference color to reduce a color change to level orange.In general, mica plate
compared to the interference color high mineral tablets.
3) quartz wedge
In the orthogonal polarizing filter was inserted between the quartz wedge, on the thin
to thick sequentially presenting a level to the four level of interference colors.In the
mine from thin to thick plate is inserted into the quartz wedge, when the same radius
are parallel, the minerals in the order of interference colour gradually increased;
synonym radius are parallel, the order of interference colour gradually reduce.
5.9 Conoscopic Observation
Use 20X to 100X objectives。
1) Engage the polarizer and analyzer for extinction position
2) Swing the condenser top lens into the light path.
3) Revolve the Bertrand lens dial to ”B” position to engage the lens into the light path.
4) Open the aperture iris diaphragm to largest size.
5) Revolve the focusing dial of the Bertrand lens to focus on the conoscopic image.
Note: If the periphery of the conoscopic image is dark, move the condenser vertically
to find the position where the periphery is brightest.
5.10 Replacement for bulb
5.10.1 The lamp bulb for reflected lighting
1) The applicable lamp bulb is the 12V50W.(PHILIPS)
2) Fully loosen the clamping screw ①at the top of the lamp housing using the
provided hexagon wrench S3.(Fig. 1)
3) Remove the lamp housing②by lifting it up.
4) Tilt the bulb socket by 90°(Fig. 2)

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5) While pushing down the bulb clamping levers, hold the halogen bulb with gloves
or a piece of gauze, insert the bulb pins into the sections as far as they will go.
Then return the lamp clamping lever gently back to the original position to clamp the
bulb. (Fig. 3)
Caution for Bulb Replacement During or RightAfter Use
When using, or soon after it is turned off the lamp, the lamp house and nearby parts
will be very hot and will cause serious burns. Please turn the main switch on “O” (off),
pull out power plug, and make sure the bulb, the lamp room and periphery are all cool.
Then, you can do your replacing.
Please insert the lamp gently, or it will be damaged by excessive extrusion.
Do not touch the Halogen bulb with your bare hands. It will shorten the service life
or cause it to burst. If you leave finger marks on the surface carelessly, clean it with a
dry soft cloth.
When using, the temperature of the lamp housing surface will be very hot, please
pay attention to the warning written on the Warning board.
5.10.2Replacement for bulb
1) The applicable lamp bulb is the 12V20W.
2) Turn off the power supply (turn the switch to “O”) and pull out the connector Wait
for 30 minutes till the bulb is cooling.