BestScope BS-2094C User manual

Microscope Instruction Manual
BS-2094C/BS-2094CF
BS-2094C BS-2094CF
This instruction manual is intended for users of the Inverted Biological Microscope BS-2094C and BS-2094CF. To
ensure safety use, perform the best performance and to familiarize yourself with the use of this microscope,
please read this manual carefully before operating this product.

Contents
Before Using ……………………………………………………………………
1
Safety Symbol…………………………………………………………………
1
Safety Precautions ……………………………………………………………
1
Care and Maintenance…………………………………………………………
2
Chapter 1 Introduction …………………………………………………………
4
1.1 Technical Specifications …………………………………………………
4
1.1.1 Main Technical Specifications and Configuration……………………
4
1.1.2 Objective Parameters…………………………………………………
5
1.1.3 Electrical Parameters…………………………………………………
5
1.2 Components ………………………………………………………………
6
Chapter 2 Overview of Agencies ………………………………………………
8
Chapter 3 Regulation and Operation …………………………………………
10
3.1 Turning the Power on ……………………………………………………
10
3.2 Adjusting the Illumination Brightness……………………………………
10
3.3 Specimen Placement and Stage Knob Adjustment………………………
10
3.3.1 Mechanical Stage……………………………………………………
10
3.3.2 Pallet…………………………………………………………………
11
3.3.3 Auxiliary Stage………………………………………………………
11
3.4 Adjusting the Interpupillary Distance………………………………………
11
3.5 Focusing on the Specimens ………………………………………………
12
3.5.1 Focus Knobs…………………………………………………………
12
3.5.2 Focus Knob Tight Adjustment ………………………………………
12
3.6 Adjusting the Diascopic Illumination Aperture Diaphragm………………
13
3.7 Switching the Objective……………………………………………………
13
3.8 Operation the Phase Contrast Microscope…………………………………
14
3.9 Operating the Episcopic Illumination Microscopy………………………………
15
3.9.1 Eprscopic Illumination………………………………………………
15
3.9.2 Fluorescence Filter Cube Turret ……………………………………
15
3.9.3 Filter Cube Switching Unit …………………………………………
15
3.9.4 Fluorescence Filter Cube……………………………………………
16
3.9.5 Fluorescence LED Unit ……………………………………………
16

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3.10 Operating the LCD ……………………………………………………
17
3.10.1 The status of LCD …………………………………………………
17
3.10.2 Operating the illumination switch ………………………………
17
Chapter 4 Assembly………………………………………………………………
18
4.1 Installation diagram ……………………………………………………
18
4.2 Installation Steps ………………………………………………………
19
4.2.1 Installing the Condenser ……………………………………………
19
4.2.2 Installing the Phase Contrast Slider …………………………………
19
4.2.3 Installing the Moving Ruler …………………………………………
19
4.2.4 Installing the Objective ………………………………………………
20
4.2.5 Installing the Eyepiece ………………………………………………
20
4.2.6 Installing the Camera…………………………………………………
20
4.3 Assembly Episcopic Illumination Microscopy…………………………………
21
4.3.1 Installing the Filter Cube ……………………………………………
21
4.3.2 Installing the Fluorescence LED Unit ………………………………
21
4.3.3 Replacing the Fuse …………………………………………………
21
Chapter 5 Maintenance and Storage……………………………………………
22
5.1 Cleaning …………………………………………………………………
22
5.1.1 Cleaning the Lenses …………………………………………………
22
5.1.2 Cleaning Parts other than Lenses ……………………………………
22
5.1.3 Cleaning off Immersion Oil …………………………………………
23
5.1.4 Decontaminating this Product ………………………………………
23
5.2 Storage ……………………………………………………………………
23
Chapter 6 Troubleshooting ……………………………………………………
24
6.1 Optical System ……………………………………………………………
24
6.2 Mechanical Parts …………………………………………………………
24
6.3 electrical Parts………………………………………………………………
25

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Before Using BS-2094C
Safety Symbol
Symbol
Description
The surface is hot and cannot be touched with bare hands.
Please read this manual carefully before using the product. Incorrect
usage can cause personal injury or property damage.
The main power switch is turned on.
The main power switch is turned off.
Adjustment direction of illumination.
Ground connection.
Up and down light switch.
Light switch.
Through the CE certification.
Lever in, Bino: Trino=100:0
Lever out, Bino: Trino=20: 100
Safety Precautions
1. Be careful when opening box, prevent lens glass to stick to fingerprint, perspiration other
factors influence observation, prevent lens and other attachment drop damaged.
2. Avoid placing the microscope in place with direct sunlight, high temperature or high
humidity, dust, and strong vibration. Ensure that the stage is flat, horizontal and strong
enough.
3. If a bacterial solution or water spatters spill on the stage, objective or observing tube,
immediately unplug the power cord. Then,wipe off any solution or water with to keep the
microscope dry. Otherwise, it could damage the instrument.
4. To avoid blocking the natural convection air for cooling, make sure the left,
right, top, and rear of the microscope are at least 10 centimeters away from the wall and
other objects.

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BS-2094C
5. Grounding the machine to avoid lightning strike.
6. To ensure safety, always make sure the main switch is in "O" (disconnecting) and cut off
the power while waiting for the LED lights and the lamp room to cool completely. Input
voltage check: the input voltage indicated on the back of the microscope is consistent with
the supply voltage, otherwise the microscope will be seriously damaged.
7. Use our company dedicated wire.
8. When carrying this product, one hand should firmly hold it by gripping the recess in the
front lower part of the main body and the other holds the recess in the rear of the main
body.
★Do not hold any other parts (such as the upper part of the illumination pillar, focus knobs,
eyepiece tube, or stage) when carrying the microscope. Doing so might result in dropping or
failure of this microscope.
Care and Maintenance
1. All lenses have been calibrated, please do not disassemble by yourself.
2. The structure of objective converter and coarse and fine focus mechanism are precise,
please do not disassemble easily.
3. The instrument should be kept clean, have to regular removal of dust, special attention
should be paid to do not contaminate the optical parts when cleaning.
4. The stain of the lens, such as fingerprints and grease can be wiped with a small amount of
ethyl ether (70%) and alcohol (30%) mixed solution.
Recess in the rear
Recess in the front lower part
F
F

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5. Do not use organic solvents to clean the non-optical parts of the microscope. Please use
neutral detergents when cleaning.
6. When using, if the microscope be wet liquid, should immediately cut off the power and
dry.
7. Do not disassemble any part of the microscope, which will affect the function or reduce
the performance of the microscope.
8. The instrument should be placed indoor with cool and dry, covered the dust cover when
not in use. Cover up, must wait for the lamp room to cool down.
9. The microscope usage environmental requirements:
a) For indoor use;
b) The environment temperature range: 10 ℃~ 35 ℃;
c) Maximum relative humidity: 80% at 31 ℃, linear temperature down to 34 ℃is 70%,
60% at 37 ℃, 50% at 40 ℃.
10. Microscope to store and transport environment requirements:
a) the environment temperature range: - 40 ℃~ + 70 ℃
b) relative humidity range: 10% ~ 100%
c) atmospheric pressure range: 500 hpa ~ 1060 hpa
Because the solvent such as ether and alcohol are extremely flammable, do not operate
power switch of various electrical equipment when using, and keep away from open fire, please
ensure indoor ventilation.

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Chapter 1 Introduction BS-2094C
1.1 Technical Specifications
1.1.1 Main technical specifications and configuration
Device
Technical Specifications
BS-2094C
BS-2094CF
Eyepiece
Extra Wide Field Eyepiece 10×/22
●
●
Field Eyepiece 15×/16
○
○
Field Eyepiece 20×/12
○
○
Observation head
Seidentopf Tilting Binocular Head, adjustable 5-35° inclined,
Interpupillary Distance 48-75mm, Left side camera port
●
●
Objective
Infinity plan objective 4×
●
○
Infinity plan objective 10×
○
○
Infinity plan objective 20×
○
○
Infinity plan objective40×
○
○
Phase contrast objective 4×
○
○
Phase contrast objective 10×
●
○
Phase contrast objective 20×
●
○
Phase contrast objective 40×
●
○
Infinity plan semi-apochromatic objective 10×
—
●
Infinity plan semi-apochromatic objective 20×
—
●
Infinity plan semi-apochromatic objective 40×
—
●
Nosepiece
Quintuple Nosepiece
●
●
Focusing mechanism
up 7mm, down 1.5mm, To limit up to 18.5mm
●
●
stage
250mm×170mm
●
●
Moving stage
Y 80mm, X 128mm
●
●
Plain stage
Two plain stage
○
○
Condenser
NA=0.3
●
●
illumination
Transmitted illumination: 3W LED
●
●
Fluorescent illumination: (B, G, U), 3WLED
—
●
Fluorescent illumination: V, 3WLED
○
○
Emboss contrast slider
contrast slider
○
○
Camera mount
C-Mount 1×
●
●
C-Mount 0.7×
○
○
C-Mount 0.5×
○
○
LCD
●
●
Note: ● Standard component, ○ Choose component,―Inapplicability.

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BS-2094C
Objective Choose component
No.
Technical Specifications
1
Infinity plan semi-apochromatic objective (adjustable) 20×, 40×, 60×
2
Infinity plan semi-apochromatic contrast objective (adjustable) 20×, 40×, 60×
3
Infinity plan semi-apochromatic contrast objective 4×, 10×, 20×, 40×, 60×
1.1.2 Objective parameters
Type
BS-2094C
BS-2094CF
Magnification
4×
10×
20×
40×
10×
20×
40×
numerical aperture (N.A)
0.10
0.25
0.40
0.60
0.30
0.45
0.60
Work distance (mm)
30
10.2
12
2.2
7.4
8
3.6
Cover-glass thickness
0.17
0.17
0.17
0.17
1.2
1.2
1.2
Conjugate distance(mm)
∞
∞
∞
∞
∞
∞
∞
1.1.3 Electricity parameter
Item
BS-2094C
BS-2094CF
1
Input voltage
AC100-240V; 50/60 Hz
2
Fuse Size
T500 mA; 250 V
3
LED light
3W S-LED
4
Fluorescence LED light (B light）
—
wavelength is 485nm at 3W
5
Fluorescence LED light (G light)
—
wavelength is 520nm at 3W
6
Fluorescence LED light (U light)
—
wavelength is 360nm at 3W

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BS-2094C
1.2 Components
The microscope BS-2094C components (following diagrams) .
BS-2094C
Objective
Condenser
Contrast slider
Nosepiece
Moving stage
Stage
Eyepiece
Viewing head
Camera mount
LCD
Mainframe

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BS-2094C
The microscope BS-2094CF components (following diagrams).
BS-2094CF
Viewing head
Camera mount
LCD
Mainframe
Fluorescence
illumination
Stage
Condenser
Filter Cube turret
Fluorescent baffle
Eyepiece
Nosepiece
Objective
Moving stage
Upper lighting
shading part
Contrast slider

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Chapter 2 Overview of Agencies BS-2094C
1
Condenser field diaphragm adjustment lever
7
Brightness adjustment knob
2
Condenser adjustment knob (up & down)
8
Fine focus knob
3
Condenser centering screw
9
Coarse focus knob
4
Aperture diaphragm adjusting knob
10
Power indicator
5
Nosepiece
11
Light distribution switch
6
Power switch
BS-2094C
4
1
2
3
5
6
9
7
8
10
11

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BS-2094C
1
Stage horizontal movement knob
2
Stage vertical movement knob
3
Fluorescence cube turret
BS-2094CF
3
1
2

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Chapter 3 Regulation and Operation BS-2094C
3.1 Turning the Power on
Turn on the power and turn the main switch
on the back of the microscope body to "-"
(on).
3.2 Adjusting the Illumination Brightness
As shown in the direction of rotation, the light
source is enhanced, whereas the light source
weakened.
3.3 Specimen Placement and Stage Knob
Adjustment
Load the slide holder into a mechanical stage,
and the cover glass slides downward slowly
into the grooves of the slide plate.
Rotate the stage knob to place the sample to
the desired position.
CAUTION
Be careful when changing the objective. The
objective may collide with the specimen,
because of the short working distance of
specimen observation.
3.3.1 Moving stage
The Moving stage is mounted and used on the
plain stage. The specimen can be moved in
the X-axis and Y-axis directions by operating
the stage knobs.
A 128×80 mm microplate can be placed on
the Moving stage. Attaching the different
specimen holders enables you to observe
various types of specimens.
The upper is the vertical knob, and rotates in
the direction as shown in the figure, the stage
will move forward in the vertical direction.
The lower is the horizontal knob rotating in
the direction shown in the figure. The stage
will move to left in the horizontal direction.
Power switch

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BS-2094C
3.3.2 Holders
There are a variety of holders to choose from.
NO.
Model
1
Terasaki microplate
2
Universal holder
3
54mm Petri dish holder/ Slide holder
4
60 mm Petri dish holder/ Slide holder
5
90 mm Petri dish holder
3.3.3 Auxiliary stage
Connect the auxiliary stage to the left
and right of the stage to expand the stage
width. After connecting the auxiliary
stage, the stage width is 300 mm.
3.4 Adjusting the Interpupillary Distance
By rotating the binocular part while
looking through the eyepieces, adjust the
distance between the eyepieces so that
the right and left view fields overlap to a
single image from eyepieces.
Auxiliary stage

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BS-2094C
3.5 Focusing on the Specimens
3.5.1 Focus knob
To adjust the focus，rotate the focus knobs
on the right and left sides of the microscope
to move the objective up and down.
The figure to the right illustrates the
relationship between the rotational direction
of the focus knobs and the vertical notion of
the objective.
Focus travel: from the focal point, up to 7mm
and down to 1.5mm, and the limit can be up
to 18.5mm. (As the right figure, spin out the
limit screw for about 4 mm trip to limit)
The traveling distance of the objective for
each knob is as follows:
Rotation of knob
Distance traveled
by objective
Fine focus knob: 1 rotation
0.2 mm
Coarse focus knob: 1 rotation
37.7 mm
CAUTION
Never attempt the following operations, as
they might result in product malfunction:
◆Rotating the left and right focus knobs in
opposite directions.
◆Rotating the coarse and fine focus knobs
past their limit.
3.5.2 Focus knob tight adjustment
The focus knob become tighter, when
rotating direction of the tightness
adjustment knob is shown as figure 8.
Otherwise become more relaxed
Tightness adjustment knob
Coarse focus knob
Fine focus knob
limit screw

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BS-2094C
3.6 Adjusting the Transmitted Illumination
Aperture Diaphragm
Insert a centering telescope in the eyepiece
tube, adjust the focal length, and view the
objective pupil plane (a bright circle) and
the aperture diaphragm image. Adjust the
aperture diaphragm so that the size of the
diaphragm image is 70-80% of the size of
the objective pupil plane.
Aperture diaphragm is designed for the
adjustment of numerical aperture, not the
dimmer. Typically, adjusting the aperture
diaphragm to 70% to 80% of the pupil of the
objective will result in an appropriate
contrast and a favorable image. The iris
aperture adjusting handle is marked with
the aperture of the diaphragm.
3.7 Nosepiece
Turn the nosepiece until it clicks in place,
stays in the specified position, can switch
the objective.
Before rotating the converter, check the
objective height to prevent collision with
specimens or stage.
Normally, the objective is mounted by
rotating the nosepiece clockwise (from
above the nosepiece) increasing
magnification in turn.
CAUTION
Be careful when changing the objective.
The objective may collide with the
specimen, because of the short working
distance of specimen observation.
Aperture diaphragm
open/ close lever
Aperture diaphragm image
Objective pupil plane
70-80%
100%

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BS-2094C
3.8 Operation the Phase Contrast Microscope
Phase contrast microscopy requires phase contrast (PH) objectives and PH sliders.
If PH Slider is used, it is possible to center the annular diaphragm. Turn the centering screw of the
PH slider to merge the annular diaphragm image with the phase ring image in the objective.
Decentering the phase ring and the PH annular diaphragm
Basically, the phase ring and the PH annular diaphragm should be adjusted so that
they are concentric. However, slightly decentering them will produce a shadowing
effect, resulting in a three-dimensional image.
Annular diaphragm
Centering hold
(on the side)
Hollow position
Centering hold
(on the side)
Hexagonal
screwdriver
Centering screws
Hexagonal
screwdriver

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BS-2094C
3.9 Operating the Episcopic Illumination Microscopy (for the BS-2094CF Only)
3.9.1 Episcopic illumination (for BS-2094CF)
Changing the illumination switch to switch
between episcopic illumination LED and
diascopic illumination fluorescent LED mode, see
section 4.3.2.
Fluorescent LED illumination will turn brighter,
when rotating direction of illumination
brightness knob as right figure shown.
3.9.2 Fluorescence cube turret
The fluorescence cube turret reflects the
illumination light from the fluorescence LED unit
into the objective. This turret allows you to
install up to three filter cubes (each with a
built-in dichroic mirror) and switch the filter
cube by rotating the switching unit.
3.9.3 Filter cube switching unit
Rotating the filter cube switching unit switches
the filter cube. When the filter cube is switched,
the fluorescence LED unit is switched
automatically.
Fluorescent positions (1 to 3) and a bright-field
position ( ) are alternately allocated to the
turret. The turret clicks each time a filter cube is
brought to a fluorescent or a bright-field
position. Address of the filter cube that is in the
optical path is indicated on the right and left of
the filter cube switching unit. When switching
the filter cube, check that the turret clicks and
the address indications match the intended filter
cube.
Illumination LED
ON/OFF switch
Illumination
brightness adjuster
Filter cube mounting port
Filter cube switching unit
Address indication
for filter cube
Address indication
for filter cube

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BS-2094C
position, seen from the front of the
microscope: If you select the bright-field
position, the filter cube is removed from the
optical path. The three bright-field positions
have the same function.
Rotate the turret so that the fluorescence
positions indicated by the arrows on the left
and right are the same (all points to “1” in
the right figure), indicating that the filter
cube has been switched into the optical
path.
★One-to-one corresponding relationship
between filter cube sign, fluorescent
LED light signs and filter cube should be
paid attention to, when using. (see
section 4.3.2 for the position of
fluorescent LED lights)
3.9.4 Fluorescence Filter Cube
A filter cube consists of three types of optical
components: an excitation filter (BP filter), a
barrier filter (BA filter), and a dichroic mirror
(DM).
Referring to the following items as a guide,
select a desired combination of filter cubes
according to the characteristics of the
specimen and fluorophore.
• You can select a combination of an
excitation filter and a barrier filter even if
you are using the same excitation method.
• Excitation filters, barrier filters, and
dichroic mirrors can be purchased
separately.
• Excitation filters are exposed to strong light
during operation and will deteriorate over
time. Replace the filter as appropriate.
Dichroic mirror
SIGN

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3.9.5 Fluorescent LED Unit
The BS-2094CF allows you to mount up to
three different fluorescence LED units as the
light source for episcopic fluorescence
microscopy.
When the fluorescence cube turret is
rotated, the fluorescence LED unit is
switched automatically. Therefore, mount
the fluorescence LED unit at the same
address as the filter cube to be used.