BestScope BS-2064 Series User manual

Version No.: V1.0
BS-2064 Series Biological
Microscope
Instruction Manual
BS-2064B BS-2064T
This instruction manual is for the operation guide, troubleshooting
and maintenance to BS-2064 Series Biological Microscope. Please study
this manual thoroughly before operating, and keep it with the instrument.
The manufacturer reserves the rights to the modifications by technology
development. On the basis of operation ensured, technical specifications
may be subject to changes without notice.

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Contents BS-2064 Series
Before Use
1. Components............................................................................................................................... 1
2. Assembling................................................................................................................................. 3
2-1 Assembling Scheme ......................................................................................................... 3
2-2 Assembling Steps ............................................................................................................. 4
3. Operation................................................................................................................................... 7
3-1 Set Illuminations .............................................................................................................. 7
3-2 Place the Specimen Slide ................................................................................................. 7
3-3 Adjust Focusing................................................................................................................ 7
3-4 Adjust the Focusing Tension ............................................................................................ 8
3-5 Adjust the Diopter............................................................................................................ 8
3-6 Adjust the Interpupillary Distance ................................................................................... 8
3-7 Center the Condenser ...................................................................................................... 9
3-8 Adjust the Field Diaphragm ............................................................................................. 9
3-9 Adjust the Aperture Diaphragm .................................................................................... 10
3-10 Select the Light Path .................................................................................................... 10
3-11 Use the Eye-cap ........................................................................................................... 11
3-12 Use the Color Filter ...................................................................................................... 11
3-13 Use the Oil Objective (100X) ........................................................................................ 11
3-14 Replace the Fuse.......................................................................................................... 12
3-15 Assembling and Operation of Simple Polarizing.......................................................... 12
3-16 Assembling and Operation of Disc Phase Contrast Condenser.................................... 12
3-16-1 Centering Halo.......................................................................................................... 13
3-17 Assemble and Use the TV Device ................................................................................. 14
3-18 Assemble and Use Intermediate Optical Splitter ......................................................... 14
4. Technical Specifications............................................................................................................ 15
5. Troubleshooting ....................................................................................................................... 17

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Before Use BS-2064 Series
1. Operation Notice
1. As the microscope is a high precision instrument,
always operate it with care, and avoid physical
shake during the operation.
2. Do not expose the microscope in the sun directly,
either not in the high temperature, damp, dust or
acute shake. Make sure the worktable is flat and
horizontal. Following environment is required
when operating: Indoor temperature: 5℃～40℃,
Max relative humidity: 80%.
3. When moving the microscope, use both hands to
hold its back hand-clasping ① and the front base
②, and lay it down carefully (see Fig. 1).
★It will damage the microscope by holding the
stage, focusing knob or head when moving.
4. When working, the surface of light source will be
very hot. Make sure there is enough room around
the light source for cooling.
5. Connect the microscope to the ground to avoid
lightning strike.
6. For safety, make sure the power switch is at “O”
(OFF) and power it off before replacing the bulb
or fuse (See Fig. 2), and wait until both the bulb
and bulb holder have cooled down.
★Bulb selected only: 6V/30W Halogen bulb (philips
5761).
7. Wide voltage range is supported as 100~240V.
Additional transformer is not necessary. Make
sure the power supply voltage is in this range.
8. Use the special wire supplied by our company.
9. All the power OFF devices have been set in the
position where is easy to operate.
2. Maintenance
1. Wipe the lens gently with a soft lens tissue. Carefully wipe off oil or fingerprints with
tissue moistened with a little of 3:7 mixture of alcohol and ether or dimethylbenzene.
★Alcohol and ether is flammable. Don’t place these chemicals near to fire or fire
source. Please use them in a ventilated place when turning on/off the electric device.
2. Do not use organic solution to wipe the surface of other components. Please use the
neutral detergent if necessary.
3. If the microscope is damped by the liquid, cut off the power immediately and wipe it
Fig. 1
Fig. 2

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dry.
4. Never disassemble the microscope. It will influence its function or damage it.
5. After using, cover the microscope with a dust cover.
3. Safety Sign
Sign
Signification
The surface gets hot and don’t touch it with bare hand.
Read the introduction before use. Unsuitable operation would lead to
person hurt or instrument faulty.
|
Main switch is ON.
O
Main switch is OFF.

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1. Components BS-2064 Series
BS-2064 Series Biological Microscope Components
Eyepiece
Trinocular Head
Light Path
Selecting Pole
Inner
Hexagon
Spanner
Objective
Stage
Condenser
Up-down Knob
Condenser Center
Adjusting Screw
Field Diaphragm
Adjusting Ring
Y-axis Moving
Knob
X-axis Moving
Knob
Fine Focusing
Knob
Tension
Adjustment
Knob
Coarse
Focusing Knob

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BS-2064 Series
Diopter
Adjustment Ring
Random Upper
Limit Knob
Body
Nosepiece
Condenser
Power
Switch
Fuse
Power
Socket
Light
Source
Group
Fine
Focusing
Knob
Light
Adjustment
Knob
Coarse
Focusing
Knob
FocusArm

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2. Assembling BS-2064 Series
2-1 Assembling Scheme
Following is the Assembling Scheme to describe how to assemble the components, and the
numbers denote the assembling order.
★Before assembling, make sure there is no dust, dirt or other materials which will disturb it.
Assemble carefully and do not scrap any part or touch the glass surface.
①
②
③
④
⑤
⑥
⑦
⑧
Halogen Bulb
Light Source Group
Power Cord
CX40 Body
Stage
Condenser
Trinocular Head
Objective
Eyepiece

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BS-2064 Series
2-2 Assembling Steps
2-2-1 Assemble the Halogen Bulb
Hold the bulb ① with clean glove or tissue and insert
the pins ② into the receptacles ③ thoroughly. Make
sure the bulb is vertical (See Fig. 3).
★Don’t touch the bulb with fingers. If there is a
fingerprint left on the bulb, please wipe it with
clean soft cloth.
★Before replacing the bulb, make sure to cut off the
main power and wait for both the bulb base and
bulb cooling down.
★Bulb selected only: 6V/30W Halogen bulb (Philips
5761).
2-2-2 Assemble the Light Source Group
Align the oriented pin① and power pin② on the
light source to oriented holder③ and power
socket④, and then push light source into arm
smoothly and plug it thoroughly. (See Fig. 4)
2-2-3 Assemble the Stage
(1) Loosen the lock-screw① on the stage fully. (See
Fig. 5)
(2) From a rear area of the rounded hole center on
the base, carefully ring the two “V”buttons on the
bottom of the stage into the “V”rounded groove②,
then screw down the lock screw①.
★The stage of different size and shape is available
according to customer.
2-2-4 Assemble the Condenser
(1) Rotate the coarse focusing knob① to raise stage
to the highest. (See Fig. 6)
(2) Rotate the condenser up-down knob② to lower
the condenser bracket to the lowest.
(3) Loosen the condenser lock-screw③ fully.
(4) Swing out the front lens of condenser with the
Fig. 3
Fig. 4
Fig. 5
Fig. 6

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BS-2064 Series
scale forward. Make the lock screw of condenser in
alignment with the groove of the condenser stand.
Push the condenser into the innermost of stand.
(5) Screw down the condenser lock-screw③, and
raise the condenser to the highest position with the
condenser up-down knob②.
2-2-5 Assemble the Head
(1) Loosen the head lock-screw① fully. (See Fig. 7)
(2) From a little right position, insert the coattail
interface on the bottom of head into the hole of
middle head with a little left inclined. Keep the two
eyepiece tubes forward, and then screw down the
lock screw①.
2-2-6 Assemble the Objective
Rotate the coarse focusing knob to lower the stage.
Install the objectives into the nosepiece from the
lowest magnification to the highest in a clockwise
direction. (See Fig.8)
★Search and focus the sample by low magnification
objective (5X or 10X) when operating. Then get
change to the high magnification ones according
to the observation requirements.
★When replacing the objective, rotate the
nosepiece until it sounds “ka-da”, to make sure
the objective is in the center of the light path.
2-2-7 Assemble the Eyepiece
(1) Take down the cover of eyepiece tube①. (See
Fig.9)
(2) Insert the eyepiece②into the eyepiece tube,
until touch the bottom.
Fig.7
Fig. 8
Fig.9

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BS-2064 Series
2-2-8 Connect the Power Cord
(1) Make sure the power switch is at “O” (OFF). (See
Fig. 10)
(2) Insert one end of power cord① into the power
socket② of the microscope.
(3) Insert the other end of power cord into the power
supply socket.
★Don’t use strong force when the power cord is
bended or twisted, otherwise it will be damaged.
★Use the special wire supplied by our company. If
it’s lost or damaged, choose one with the same
specifications.
★Connect the power cord appropriately to make
sure the instrument is connected to ground.
Fig. 10

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3. Operation BS-2064 Series
3-1 Set Illuminations
(1) Put through the power and turn on the main
power switch to“—”. (see Fig. 11)
(2) Adjust the light adjustment knob①until the
illumination is comfortable for observation. Rotate
the light adjustment knob① clockwise to raise the
brightness. Rotate the light adjustment knob①
counterclockwise to lower the brightness.
★Use bulbs in the low-voltage state can extend the
bulb life.
3-2 Place the Specimen Slide
(1) Push the wrench①of the specimen holder
backwards. (See Fig. 12)
(2) Loosen the wrench①, and clamp the slide②by
the clips while the cover glass faces up.
(3) Rotate the X-axis knob④and Y-axis knob③.
Move the specimen to the center (alignment with the
center of the objective).
3-3 Adjust Focusing
(1) Put the slice on the stage, and hold it down with
the clip. Shift the 4X objective into the light path. (See
Fig. 13)
(2) Loosen the random upper limit knob①, then
observe the right eyepiece with the right eye. Rotate
the coarse focusing knob② until the image appears
in the view field, then lock the random upper limit
knob①.
★The random upper limit knob can prevent the
objective touching the slice when focusing.
★The random upper limit knob does not react
on the fine focusing knob.
(3) Rotate the fine focusing knob③ for clear details.
★When observing with the 4X or 10X objective,
Fig. 12
Fig. 13
Fig. 11

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BS-2064 Series
open both the aperture diaphragm and field
diaphragm to the maximum position, and swing
out the front condenser lens. See “3-7 Center the
Condenser”for condenser operations.
3-4 Adjust the Focusing Tension
If the handle is very heavy when focusing or the
specimen leaves the focus plane after focusing, or the
stage declines itself, please rotate the tension
adjustment knob① according to the arrowhead
pointed direction. (See Fig. 14)
3-5 Adjust the Diopter
After the image is clear in the right eyepiece, observe
the left eyepiece with the left eye. Rotate the diopter
adjustment ring① until the image is clear (Fig. 15).
There are ±5 diopters on the diopter adjustment
ring①, the value aligned with the scale is your eye’s
diopter. The dot “·”on the left side can also indicate.
★Remember your eye’s diopter, so that you can use
it next time.
★The user could select any side of the eyepieces as
a standard of reference if there is diopter
adjustment ring on both eyepiece tubes, then
adjust the other one. The eyepiece which is
selected as a standard of reference must line up
with “0” before focusing observation.
3-6 Adjust the Interpupillary Distance
When using two eyes to observe, hold the bases of
the prism and rotate them around the axis to adjust
the interpupillary distance, until there is only one field
of view. (See Fig. 16)
The dot “·” on the left eyepiece base points to the
Fig. 14
Fig. 15
Fig. 16

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BS-2064 Series
scale of the interpupillary distance indicator. The scale
value is the interpupillary distance.
Adjustable range: 50~76mm.
★Remember your eye’s interpupillary distance, so
that you can use it next time.
3-7 Center the Condenser
(1) Rotate the condenser up-down knob① to raise it
to the highest position. (See Fig. 17)
(2) Rotate the spanner② to move the front lens into
light path.
★Move the front lens of condenser into light path
when the objective is beyond 20X.
(3) Move the 20X objective into light path and focus
the specimen.
(4) Rotate the field diaphragm adjustment ring③to
put the field diaphragm to the smallest position, then
the image of field diaphragm can be observed
through eyepiece.
(5) Rotate the condenser up-down knob to adjust the
image to the clearest.
(6) Adjust the condenser center adjusting screw④to
put the image to the center of the field of view.
(7) Open the field diaphragm gradually. If the image is
in the center all the time and inscribed to the field of
view, it shows condenser has been centered correctly.
(See Fig. 18)
(8) In use, you can enlarge the field diaphragm and
make the image circumscribed to the field of view.
3-8 Adjust the Field Diaphragm
By limiting the diameter of light entering the
condenser, the field diaphragm can prevent other
light and strengthen the image contrast. When the
image is on the edge of the field of view, the objective
can perform best and obtain the clearest image.
Fig. 17
Fig. 18
Fig. 19

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BS-2064 Series
3-9 Adjust the Aperture Diaphragm
The aperture diaphragm decides the numerical
aperture of the illumination system. If the N.A. of
illumination system matches with the N.A. of the
objective, it can obtain better resolution and contrast,
and increase the depth of field.
As the contrast is usually low, it is advised to adjust
the condenser aperture diaphragm to be 70%-80% of
the N.A. of objective. The eyepiece can be taken off
when it’s necessary to observe from the tube. Adjust
the aperture diaphragm adjusting ring③ until see
the figure as shown in Fig. 19, to adjust the
proportion (see Fig. 19&20, ① is the image of
aperture diaphragm, ② is the edge of objective).
Use of scale: set the scale of condenser N.A. to the
80% value of objective④ N.A.
For example, use 40X objective (N.A. 0.65), set the
scale of aperture diaphragm to 0.65 x 0.8=0.52.
3-10 Select the Light Path
For trinocular head, the light path selecting pole①
control the light-energy ratio of binocular and
trinocular. When the light path selecting pole is
pushed to the innermost, all the light will enter the
binocular head; when it is pulled to the outmost, the
ratio of binocular and trinocular is 5:5. Usually, push
the selecting pole to the innermost for binocular
observation, and must pull the selecting pole to the
outmost for trinocular observation (TV&Photography).
(See Fig. 21)
Fig. 20
Fig. 21

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BS-2064 Series
3-11 Use the Eye-cap
(1) Turn over the eye-cap if the user is wearing glasses,
so that it can prevent the glasses touching the
eyepieces and avoid damaging to both glasses and
eyepieces.
(2) Open the eye-cap if the user doesn’t wear glasses,
so that it can prevent stray light disturbing the
observation. (See Fig. 22)
3-12 Use the Color Filter
The color filter can make the background light more
suitable and strengthen the image contrast (Fig. 23).
There are four colors of filter selectable: blue, green,
yellow and white.
★Place the rough side of filter downwards.
3-13 Use the Oil Objective (100X)
(1) Use the 4X objective to focus the specimen.
(2) Place a drop of oil①on the specimen (see Fig.
24).
(3) Rotate the nosepiece counterclockwise and rotate
the oil objective (100X) to the light path. Then use the
fine focusing knob to focus.
★Make sure there is no air bubble in the oil for fear
affect the image.
A. Move the eyepiece to examine the air bubble.
Open the aperture diaphragm and field diaphragm
fully and observe the edge of the objective from the
tube (It seems round and light).
B. Rotate nosepiece slightly and swing the oil
objective for some times to remove the air bubble.
(4) After using, wipe the front lens with a tissue
moistened with a small amount of 3:7 mixture of
Fig. 22
Fig. 23
Fig. 24

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BS-2064 Series
alcohol and ether or with dimethylbenzene. Wipe off
the oil on the specimen.
★Don’t put another objective to the light path
before the oil is wiped to avoid wetting the dry
objective.
★Too much dimethylbenzene would dissolve the
lens’s stickiness.
3-14 Replace the Fuse
Turn the main switch to “O” (OFF) before replacing
the fuse. Pull out the power cord. Then screw off the
fuse group① from the fuse base②with a “-” type
screwdriver. Install a new fuse and screw it in the fuse
base (See Fig. 25).
★Specifications of the fuse: 250V, 3.15A.
3-15 Assembling and Operation of Simple Polarizing
Simple polarizing system includes polarizer④and
360°rotatable analyzer③.
(1) Unplug the dust-cover① from the socket② of
front body, and insert the 360°rotatable analyzer③
into the socket②. (See Fig. 26)
(2) Put the polarizer④into the condenser⑤ groove.
(3) Rotate the 360° rotatable analyzer③can start
simple polarizing observation.
★When view field of eyepiece is the darkest, the
polarizer④ and 360° rotatable analyzer③are in
the polarized orthogonal state.
3-16 Assembling and Operation of Disc Phase Contrast
Condenser
Assembling of Disc Phase Contrast Condenser refers
to 2-2-4 Assemble the Condenser.
There are each magnification ring diaphragm and
bright field “BF”hole positions on the phase contrast.
Fig. 25
Fig. 26
Fig. 27

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BS-2064 Series
ring①. In phase contrast observation, ring
diaphragm magnification of phase contrast ring①
should match with the phase contrast objective, and
center the ring diaphragm. In bright field observation,
rotate the phase contrast ring①to “BF”position.
When it sounds “ka-da” in rotating, it indicates that
one diaphragm or hole is rotated into the center of
optical path (see Fig. 27).
3-16-1 Centering Halo
(1) Rotate the 10X phase contrast objective into the
light path, then rotate the ring diaphragm of phase
contrast ring① to the 10X position.
(2) Turn the aperture diaphragm lever② to the
leftmost.(Always keep the aperture diaphragm to the
maximum in phase contrast observation.)
(3) Put specimen on the slice clip and focus.
(4) Take out one observation eyepiece and replace it
with a CT (centering telescope) into the tube without
diopter adjustment.
(5) Loosen the lock screw of the centering telescope,
move the telescope tube up and down, to adjust the
image of the halo④ and phase ring⑤ to be clear,
and then lock the screw. (See Fig. 27 & 28)
(6) Adjust the phase contrast ring adjusting lever③
when observing from the centering telescope, and
center the halo④ with the phase ring⑤.
(7) Take off the CT, and insert the eyepiece, to observe
the phase contrast effect.
(8) Adjust phase contrast objectives of other
magnification and ring diaphragm according to above
steps.
★It cannot get the best phase contrast observation
effect if the halo is not centered correctly.
★Center the brightest halo to the objective phase
ring if double image appears.
Fig. 28

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BS-2064 Series
3-17 Assemble and Use the TV Device
(1) Loosen the lock screw① of trinocular head, and
take out the dust-cover②(See Fig.29).
(2) Take off the dust-cap of the TV adapter③. Insert
the TV adapter into the trinocular head as shown in
the figure and screw down the lock screw①.
(3) Loosen the lock screw④ of the TV adapter. Take
down the vidicon interface (C type) ⑤ from the TV
adapter, and screw into the CCD or vidicon. Then
install the interface into the TV adapter, and screw
down the lock screw④.
(4) For binocular observation, pull the light path
selecting pole⑥ to the outmost and observe the
image. If the image is unclear, rotate the adjustment
tube⑦until it is clear.
3-18 Assemble and Use Intermediate Optical Splitter
(1) Loose the lock-screw ① completely. (See Fig. 30)
(2) From a little right position, insert the coattail
interface on the bottom of intermediate optical
splitter ② into the hole on the stand with a little left
inclined, and keep the TV device interface on the left
side of the body. Then screw down the lock screw①.
(3) Loose the lock-screw ③ completely.
(4) Insert the middle coattail on the bottom of the
head④to the round hole of the intermediate optical
splitter from its center a little right. Keep the two
eyepiece tubes ahead. Screw down the lock-screw①.
(5) Insert the TV device ⑤ into the intermediate
optical splitter ② as shown in Fig. 31. See 3-17
Assemble and Use the TV Device for more details.
(6) For binocular observation, pull the light path
selecting pole⑥ to the outmost and observe the
image. If the image is unclear, rotate the adjustment
tube until it is clear.
Fig. 30
Fig. 31
Fig. 29

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4. Technical Specifications BS-2064 Series
4-1 Key Technical Specifications of BS-2064 Series Biological Microscope
Optical
System
Color corrected infinity optical system
Head
Gemel Binocular head, 30°inclined, 360°rotatable.
Gemel Trinocular head, 30° inclined, 360° rotatable, Splitting ratio: Binocular
Head 100%, Binocular Head/ Trinocular Head 50%/50%.
Gemel Binocular head, 30°~60°inclined adjustable, 360°rotatable.
Eyepiece
PL10X high eye-point plan eyepiece, line field of view: 22mm.
Nosepiece
Reversed quintuple nosepiece
Objective
Infinity plan achromatic objective(4X, 10X, 20X, 40X, 100X ),
Infinity plan phase contrast objective (10X, 20X, 40X, 100X )
Focus
Coaxial coarse & fine focusing system with limit-stopper & tension adjustable.
Travel rang: 30mm. Height-adjustable bracket group stage.
Fine focusing precision: 0.002mm
Stage
Double-layer mechanical moving stage. Area: 185×165mm. Moving range:
80x55mm. Accuracy: 0.1mm. Superhard oxidation metal platform, left or right
hand position is optional.
Double-layer mechanical moving stage. Area: 175×145mm. Moving range:
75x50mm. Accuracy: 0.1mm. Left or right hand position is optional.
Condenser
NA1.2/0.22 Shake-out Achromatic condenser
NA1.25 five-hole disc phase contrast condenser
NA0.9 Dry dark field condenser
NA1.25 Oil-immersed dark field condenser
Illumination
System
100-240V wide range of voltage, transmission and reflection Koehler
illuminator systems, 6V/30W Halogen bulb, filament center and brightness
continuously adjustable.
Polarizer
The analyzer vibration direction is 360°adjustable. Both the polarizer and the
analyzer can be moved out of the light path
Filter
Yellow, green, blue, and neutral filter.

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BS-2064 Series
4-2 Parameters of Objective
Type
Magnification
Numerical
Aperture
(N.A)
Operating
Range
(mm)
Conjugate
Distance
(mm)
Parfocal
Distance
(mm)
Thickness
of Cover
Glass
Magnification
Mark
(color ring)
Infinity
plan
achromatic
objective
4X
0.10
11.9
∞
45
0.17
Red
10X
0.25
12.1
∞
45
0.17
Yellow
20X
0.40
1.56
∞
45
0.17
Light green
40X(S)
0.65
0.36
∞
45
0.17
Light Blue
100X(S)(OIL)
1.25
0.18
∞
45
0.17
White
Infinity
plan phase
contrast
objective
10X
0.25
12.1
∞
45
0.17
Yellow
20X
0.40
1.56
∞
45
0.17
Light green
40X(S)
0.65
0.36
∞
45
0.17
Light blue
100X(S)(OIL)
1.25
0.18
∞
45
0.17
White