BestScope BS-5040B User manual

Polarizing Microscope
Model Number: BS-5040B/T
Instruction Manual
BS-5040B BS-5040T
To ensure the safety and obtain satisfactory performance and to familiarize yourself fully with the
use of your microscope, we recommend that you read this manual thoroughly before operation.
Retain this instruction manual in an easily accessible place near the work desk for future reference.

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CONTENT
Attentions
2
…………………………………………………………………………
1. Parts Name
3
………………………………………………………………
2. Performance Parameter
4
………………………………………………………………
3. Installation And Carry
5
………………………………………………………………
4. Operation And Use
6
………………………………………………………………
5. Maintenance
7
…………………………………………………………………………
6. Troubleshooting
9
…………………………………………………………………………
7. outfits
10
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ATTENTIONS
1. Purpose
This microscope is used only for microscopic observation, can not be used for other purposes.
Otherwise may cause the damage of the instrument.
2. Disassembly only by the professionals.
The microscope has been adjusted before shipping, Unprofessional-person should not
disassemble and remove any other parts.
If you have any questions, please contact with manufacturer or local distributor.
3. Note the input voltage if identical with operating voltage of instruction.
This instrument designed for wide input voltage (100V~240V, 50/60HZ), applicable to most
area. But if the supply voltage exceeds this range, the instrument will be seriously damaged.
4. Use in safe way, prevent burns and fire.
When the Instrument power is in working, temperature of bulb and lamp house
will rise sharply to meet the heat balance, so pay attention to anti-hot logo in case
of be burned.
Do not use alcohol, gasoline, paper and other combustibles near the instrument, to prevent a
fire! !
5. Notes on replacing the bulb.
The instrument used with 6V/20W or 6V/30W halogen lamp of G4, LED model with 3.4V/3W,
replacement should be used with same specifications of the bulb, otherwise may cause
equipment damage.
Before replacing the lamp, must turn off power switch until bulb is cool off, and unplug it in
case of electric shock.!
6. Requirements for handling and using environment
Please cut off the power and unplug the power plug before moving. Be careful not to crush
your finger when placed.
This instrument is a precision instrument, and it should be handle with care, severe shock can
cause serious damage to the inner related parts.
The required available environment for using of the equipment:
Indoor temperature: 0 ℃~ 40 ℃Maximum relative humidity: 85%
High temperature or high humidity may cause mildew, fog or dew of the optical components,
will result in the instrument not work.
7. For the protection of the environment, please properly handle the microscope packing waste
(such as: cardboard, foam, etc ).

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1. Parts Name
Quartz Wedge
Bertrand Lens
Nosepiece
Analyzer change
-over handle
Power Fuse Holde
r
Power Switch
Condenser
focusing knob
Left Coarse & Fine
focusing adjustment
knob
Body Handle
Coarse & Fine tension
adjustment knob
Centering Nosepiece
Eyepiece
Non-stress Objective
Locking Screw
Condenser
Collector
Eyepiece Tube
Body Handle
Right Coarse & Fine
Focusing Knobs
右粗微动
调焦手轮
Brightness
control knob
λ,λ/4
Compensator
Built-in Polarizer
Clamps
Analyzer driving
lever
Stand
Midbody
Center Adjustable
rotating Stage
Diopter Adjusting
Ring

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2. Performance Parameter
2.1 Magnification (〝O〞is optional)
Objective
Eyepiece
4×
10×
20×
40×
60×(O)
10×
40×
100×
200×
400×
600×
2.2 Objective (〝O〞is optional)
Objective
Field(mm)
Infinity Plan
Achromatic Objective
Numerical
Aperture
N.A.
FOVφ18
Resolution
(μm)
Working
Distance(mm)
4×
0.10
4.5
2.8
6.73
10×
0.25
1.8
1.1
4.19
20×(s)
0.40
0.9
0.69
2.14
40×(s)
0.65
0.45
0.42
0.45
60×(s) (o)
0.80
0.3
0.40
0.19
2.3 Eyepiece
Item
Magnification
FOV（mm）
Reticule cross
10×
18
Normal eyepiece
10×
18
2.4 Other Parameters:
(1) Mechanical Tube Length: 160 mm
(2) Viewing Head : Inclined 30°, Seidentopf Binocular(Trinocular) head, Interpupillary
Distance 50～75mm; Dioper Adjusting ±5 ,With antifungus device.
(3) Midbody: Analyser 360°, Division 2°30′,With locking device. Bertrand lens with center
Adjustable, Combining slip with Gypsum (λ) and Mica (λ/4) (Quartz Wedge Is Optional).
(4) Nosepiece: Quadplex, Center Adjustable.
(5) Mechanical stage: Revolving Stage, Diameter Φ158 mm, 360° Uniform Scale, Vernier
Division 6′.
(6) Focusing systems: Coaxial Coarse And Fine Focusing Knobs, Coarse stroke 22mm, up
position is optional; Fine division 2µm, Condenser up-down range 22 mm.
(7) Condenser: Abbe Condenser N.A.1.25, Adjustable Aperture, Aperture Center Can Be
Adjustable, With Polarizer.
(8) Electric components: Input voltage AC100-240V, 50/60Hz.
Output voltage DC1.2V～6V .
Halogen lamp 6V/20W, 6V/30W or 3.4V/3W LED.
Fuse 2A Φ5X20 .
(9) Filter: Blue (Amber, Green, Neutral Is Optional).

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3.Installation And Carry
3.1 Installation Process
3.2 Carry
Microscope is precision instrument, careful in carry. Shut down power supply, pull out power line.
Lock the eyepiece tube and condenser etc, no slice left on stage. Don't move nosepiece, focusing
knob, mechanical stage and eyepiece tube etc, don't make the eyepiece off. Avoid stumble into the
4. take out socket head wrench for use whenever appropriate.
5. take out main body, loosen locking screw, put the seidentopf binocular(trinocular)
head aside.
6. take out midbody, align connector to main body according to fixed direction,
locking fastening screw.
7. plug seidentopf binocular(trinocular) eyepiece tube in midbody, lock it with socket
head wrench.
8. Pull down dust cover, insert two eyepieces in tube, check if in good position.
9. Pull down nosepiece’s dust cover, take out objective and install in sequence for
use.
10. Rotate Coarse focusing knob, lower rotating stage, screwing 10X objective in
objective hole which on the nosepiece’s outer ring with yellow mark, then switching
clockwise the nosepiece, in proper order to install objective.
11. Place filter, compensator and nosepiece centering wrench on side for use.
12. Check instrument if in good condition, switch on and turn on the power, following
operation method to check it if in normal operating condition.
Installation complete!
▲During installing eyepiece, Please screw eyepiece and wrench nosepiece’s cover,
not wrench eyepiece directly.
▲When leaving factory, objective hole with yellow mark has be regarded as basic
hole, centering wrench hole has been sealed, should install 10X objective (yellow
mark line).
▲Please properly dispose packing waste after installation complete.
▲Microscope is precise instrument, so installation should be careful in case
scratching optical lens.
▲Please don’t touch eyepiece or optical lens of condenser with finger, otherwise will
effect normal operation.
1. Confirm the service environment is good for use.
2. Clean working desk, put microscope parts in the left side.
3. Dispose the packing waste properly, don’t left plastic bag or foam on the working
desk.

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chair, violent shocks and collision can cause damage.
a. Single hand carry way: with one hand holding the body, the microscope will be hold by thumb
buttoned filed from inside.
b. Two hands lift way: one hand turn back the microscope slightly by holding body handle, the
another hand holding the microscope’s forepart.
4. Operation And Use
Bright field operation process instruction
△When inspecting to confirm polarizing direction, please finding a cleavage black mica from
mineral slice and place it in center of field, then rotate stage again. Stop rotating when black
mica’s color become very dark, the direction of cleavage seam just present the polarizing
direction.
△In positive cross polarized light, if interference colours of mineral slice is first class grey ,after
insert gypsum (λ) in optical path, please change to second class blue-green if homology radius
is parallel; if synonyms radius is parallel, change first class grey to first class orange color.
△In positive cross polarized light, if interference colours of mineral slice is first class purplish
red ,after insert mica(λ/4) in optical path, please change to second class blue; if synonyms
radius is parallel, lower to first class orange color.
△Analyzer change-over handle can convert optical path, switching observation from mono
polarized light to positive cross polarized light.
△Midbody, analyzer, compensator and Bertrand lens bearing.
1.Compensator socket: can plug in gypsum (λ), Mica (λ/4) or Quartz wedge.
Turn power switch, rotating screw to adjust
brightness of Field.
Place specimen on stage, moving to the light path
until to observe position, gently press clamps,
moving to slice’s both end then fixed.
Rotate nosepiece, put objective10X in light path;
rotate Coaxial Coarse And Fine Focusing handle
wheel to focusing until legible image.(adjust
tension ring to adjust coaxial coarse and fine
focusing circle’s tension.
Polarizing direction is reseted in horizontal level.
(can through complete cleavage black mica
sheet to define polarizing direction.
When polarizer and analyzer located in dial 0°, is
orthogonal each other and FOV will be dark(no
sample),if not, should check polarizing position.
According to mineral slice interference colors, plug
in slider.
1. Switch on the power, adjust brightness.
2. Place Mineral Specimen.
3. Put 10X objective into optical path and
focus.
4. Define polarizing direction.
5.Check polarizer and analyzer is
orthogonal.
6. Use compensator slider.
Observing
7. Observe with Bertrand lens
Conoscopic observation should use high power
eyepiece, push in Bertrand lens when in
orthogonal polarization, to determine mineral’s
optical sign and optic axial angle.

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2.Bertrand lens conversion inserted block: pulling it can convert Bertrand lens in light
path to make conoscopic observation.
3.Gypsum (λ) slip (first class red), Orthogonal polarizing appear first class purplish red
interference colours.
4.Mica (λ/4) slip, Orthogonal polarizing appear first class grey colours.
5.Quartz wedge, Orthogonal polarizing appear first class to four class interference colours from
thin to thick.
5.Maintenance
5.1 Clean
(1) Don’t touch the lens with hand, Dust on lens should be cleaned by soft brush or absorbent
cotton or cleaned by absorbent cotton, lens paper with the mixture of alcohol and ether
(proportion 1:4).
(2) Alcohol and ether all are burnt easily, please take them away from fire. Be careful for turn
on and off power.
(3) Don’t clean painted metal and galvanizing metal with organic solvent such as alcohol, ether
or the mixture of the both. Silicon cloth or soft cleaning preparation is suggested to clean
it.
(4) Plastic should be cleaned by soft cloth with clear water.
5.2 Application environment
(1) Should be in cold and dry environment , without shaking ,dust and steam without
acid-base and other corrosive gas.
(2) Normal operation condition: indoor temperature: 0℃～40℃, maximum relative
humidity: 85%.
(3) High humidity area, recommend with dehydrating unit in case of mildew and frog.
5.3 Bulb Replacement
A . Replacement of halogen.
▲Please use specified bulb in case of damage or fire.
Specified bulb：halogen 6V/20W or 6V/30W, LED 3.4V/3W
LED(According to outfits）.
(1) shut off the power (switch to "○" side), unplug the power
socket;
(2) waiting about 30 minutes until lamp box is cold.
▲Please be sure that the bulb is cool, then follow by the nest operations.
(3) Lay aside the microscope reliably, unscrew the knurled thumb screw of the lamp housing
cover on the underside of base.
(4) Pull the knurled thumb screw gently outward, turn out lamp holder flap.
(5) Confirm the bulb is cold down, Pull out the old bulb carefully.

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(6) Hold a new bulb with silk cloth to avoid fingerprint and dust affect bulb brightness and
service life, and insert fully the contact pins into the bulb socket.
▲Before replace bulb, confirm if bulb connected strongly with the power cable in case of bad
contact.
▲Plug fully contact in bulb socket, or will result in short circuit.
(7) Push back the lamp holder flap, tighten the knurled thumb screw.
▲After working 10 hours，suggesting the suspension of using about half an hour for continue
using.
B. Replacement of LED
（1）Shut off the power
Turn the power switch off, and pull out power wire.
（2）Replacement of LED
In reference to the replacement of halogen,(different
luminescence way, different way of lamp holder).when replace
3W LED, pull out lamp holder and loosen screw to take off damaged lamp, then insert new
into the holes of light rack.
（3）Instrument restoration
According to reverse order, restoring the instrument, cleaning the working stage and adjust
light source for operation.
5.4 Replacement of fuse
(1) First shut off power, pull out the plug.
(2) Unscrew fuse tube socket in back of base, take out old fuse.
(3) Replace with same specification fuse，then plug in fuse tube socket again.
5.5 Not in use:
(1) Please shut off the power , covered by dust cover, keep in dry and cool place.
(2) The eyepiece and objective should be placed in dry containers (such as moistureproof
cylinder) with desiccant.
▲For better performance index, suggest checking the instrument regularly.
Please keep in touch with local agency if finding trouble need maintenance.

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6. Troubleshooting
In the period of using BS-5040 series microscopes, if there is any trouble occurs, please referring
to the following sheet listed some common troubleshooting resolve them or keep in touch with
us or local agency.
Trouble
Causation
Remedy
No bulb
Install bulb
Plug is unreliable
Check joint again
Bulb is broken
Replace bulb
Switch on but bulb dark
Fuse is broken
Replace fuse
Bulb is unstable
Insert again
Bulb is flickering or
brightness is unsteady.
Bulb is broken
Replace bulb
Bulb specification doesn’t meet the
requirement.
Replace bulb
Bulb brightness is low.
Rotate potentiometer to
adjusting brightness.
Objective isn’t in correct optical
path.
Rotate the objective in correct
position.
The size of iris aperture is too
Small.
Adjust the size of iris aperture.
Lens (objective, eyepiece,
condenser, light collector) has
dust.
Clean it
Brightness of view field
isn’t enough or is
uneven.
Position of Condenser is too low .
Adjust condenser properly.
Cover glass of specimen doesn’t
meet the requirement.
Use required thickness cover
glass (0.17mm).
Cover glass of specimen isn’t in up
direction.
Place specimen correctly.
Surface of objective lens Is dirty
(especially it is easy for the front
lens of 40× objective to dip in
immersion oil).
Clean it
Immersion oil isn’t used for
100× objective (oil)
Use immersion oil
Immersion oil doesn’t meet the
requirement.
Use immersion oil supplied by
us.
There is bubble in immersion oil.
Clear the bubble away
Size of iris aperture isn’t proper.
Adjust the size of iris aperture.
Image is not clear
(contrast or definition is
not enough)
Position of condenser is too low.
Readjust again
Objective isn’t in correct
optical path.
Make the objective in correct
position..
One side of image is
dark or image is
moving as focusing.
Specimen isn’t placed correctly.
Place specimen levelly on
stage and clip it with clamp.
Dirt and dust can be
Dirt or dust on bulb glass
Clean it

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Dirt or dust on specimen
Condenser’s front lens has dust or
dirt.
seen in Field of view.
Lens (objective, eyepiece,
condenser, light collector) has
dust.
Cover glass of specimen
doesn’t meet the requirement.
Place specimen correctly.
Objective touching
specimen as changing
low power to high
power
Cover glass is too thick.
Use required thickness cover
glass (0.17mm).
Interpupilary distance isn’t
adjusted correctly.
Adjust Interpupilary distance.
Diopter isn’t adjusted correctly
Adjust diopter
Image observed by two
eyes aren’t in
superposition entirely.
Left and right eyepiece is different.
Replace same eyepieces.
Interpupilary distance isn’t
adjusted correctly.
Adjust Interpupilary distance.
Diopter isn’t adjusted correctly.
Adjust diopter
It is easy for eyes to be
tired during observing.
Brightness isn’t enough
Adjust brightness
Outfits
Items
Specification
BS-5040B
BS-5040T
WF 10×-18mm
●
●
Eyepiece
WF 10×-18mm (Reticule 0.1mm)
●
●
4×
●
●
10×
●
●
20× (S)
●
●
40× (S)
●
●
Non-stress
Infinity
Plan Objective
60× (S)
○
○
Seidentopf Binocular
Head
Inclined 30°, Rotatable 360°, Interpupilary
Distance: 50-75mm.
●
Seidentopf Trinocular
Head
Inclined 30°, Rotatable 360°,
Interpupilary Distance: 50-75mm.
Light Distribution 20:80
●
Nosepiece
Quadplex, Center adjustable
●
●
Analyzer Unit
0-90°，It can be moved out of the optical path
for single polarizing observe.
●
●
Bertrand Lens
It can be moved out of the optical path.
●
●
λSlip
First Class Red
●
●
1/4λ Slip
●
●
Quartz Wedge
(Ⅰ-ⅣClass)
●
●
Revolving Round Stage
Rotatable 360°, Division 1°, Vernier division 6’，
can be locked.
●
●
Polarizing Attached Mechanical Stage
○
○
○
Condenser
Abbe N.A. 1.25 , Non-stress
●
●
Polarizing Unit
It can be moved out of the optical path.
●
●
Illumination
6V/20W Halogen Lamp
●
●

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6V/30W Halogen Lamp
○
○
3W-LED Lamp
○
○
Blue(Built in)
●
●
Amber
○
○
Green
○
○
Filter
Neutral
○
○
C-mount
1X, 0.47X （Focus adjustable）
○
○
Note: "●"In Table Is Standard outfits, "○" Is Optional Accessories.