BestScope BS-2010BD User manual

Bestscope International Limited
Digital Binocular Microscope
BS-2010BD
Instruction Manual
This manual is written for digital binocular microscope BS-2010BD. To ensure the safety, obtain optimum
performance and to familiarize the use of this microscope, it is recommended strongly that you study this
manual thoroughly before using the microscope and retain this manual in an easily accessible place near
the work desk for future reference.

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1. Brief introduction
Digital binocular microscope BS-2010BD is widely used in biological, pathological, bacteriological
fields for purposes such as observation, research, academic demonstration, clinic, medical inspection. It
features more comfortable and reliable operation with modern design and advanced technology.
Brightness adjusting wheel
Main switch
Fine focusing knob
Coarse focusing knob
Limit bolt
Collector
Condenser
Stage
Objective
Eyepiece
Digital Binocular head

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2. Main Specifications
1. Mechanical tube: 160mm
2. Objectives (achromatic)：
Magnification
Numerical
Aperture
Focal Length
f (mm)
Working
distance (mm)
4X
0.10
31.05
37.5
10X
0.25
17.13
7.316
40X
0.65
4.65
0.632
100X
1.25
2.906
0.198
3. Eyepiece
Category
Magnification
Focal Length f(mm)
Field of view（mm）
Plan Eyepiece
10X
24.95
Ø 18
4. Total Magnification
Eyepiece
10X
10X
10X
10X
Objective
4X
10X
40X
100X
Total
40X
100X
400X
1000X
5. Objective conjugate distance: 195mm
6. Stage: double layer stage 125mmX115mm
7. Coarse focusing range: 6.5mm
8. Condenser: Abbe Condenser, NA=1.2 with iris diaphragm
9. Diameter of filter: Ø28mm
10. Illuminator: LED Lamp, brightness adjustable
3. Outfit
Name
Specifications
Standard outfit
Body
Stand
O
Double-layer mechanical stage
O
Base (LED lamp)
O
Viewing Head
Binocular digital head
O
Condenser
NA1.2 Abbe Condenser
O
Nosepiece
Quadruple
O
Illuminator
LED lamp
O
Eyepiece
10X Plan Eyepiece
O
Objectives
4X
O
10X
O
40X
O
100X
O
Immersion oil
5ml
O
AC Adapter
USA/European/UK plug type
O
User Manual
English version
O
Package
Foam box and carton
O

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4. Structure Feature
a) Eyepiece, objective and nosepiece
10X plan eyepieces combined with 4X, 10X, 40X, 100X achromatic objectives can give the
microscope a large and clear field of view. The nosepiece has a precise frame which can locate
objectives accurately and stably. The digital binocular head can turn 360 degree freely with
comforts and conveniences.
b) Curved arm and coarse and fine focusing frame
The C-type molding of the curved arm is sprightly. The coarse focusing frame adopts dovetail
guide and move smoothly with upper limit device.
c) Base
The base, with built-in illuminator, is the foundation of whole microscope which adopts
streamline circular in consonance with the curved arm.
d) Stage
Use double-layer mechanical stage, the slide is fixed with slide clip.
e) Illumination system
The illumination system consists of illuminator, iris diaphragm and condenser.
Abbe condenser can be raised and lowered by simple operation.
5. Assembly
A) Environmental Requirement：
1. Ambient temperature: 5°C to 40°C(41°F to 104°F), Maximum R.H: 85%.
2. High Temperature will result in a mildewing, dew and even ruinous instrument.
3. Avoid placing the instrument in a dusty environment. When ending your microscope operation,
please cover it with the dust cover.
4. Lay the microscope in a plan and stable position, please.
B) Input voltage check:
Check the input voltage: be sure the input voltage which signed in the back of the microscope
is consistent with the power supply voltage, or it will bring a serious damage to the instrument.
C) Eyepiece:
Insert the eyepiece into the eyepiece tube.
D) Objective:
Adjusting the coarse focus knob until the mechanical stage reaches its lower limit position.
Screw the lowest magnification objective into the nosepiece from the left or the right side,
then revolve the nosepiece clockwise and mount other objectives in the sequence of low to high
magnification
E) Condenser:

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The condenser had been installed in the microscope before leaving factory.
F) Collector:
The collector had been installed and located before leaving factory. The screw-mounted
collector can be taken down by anticlockwise turning.
H) Filter:
Mount the filter on the iris diaphragm bracket under the condenser if necessary.
6. Operation
1. Insert the eyepiece into the eyepiece tube and revolve the objectives into the nosepiece in
sequence of magnification.
2. Place the specimen in the center of the stage, keeping the cover slip upward, and then nip it
with the specimen holder.
3. Search the image by lower magnification objective, turn the longitudinal and transversal
adjustment knobs which on the mechanical ruler to move the specimen onto the center of the
field of view. Then replace with higher magnification objective, turn the fine focusing knob to
sharpen the image.
4. When using 100X oil objective, add the immersion oil between the objective and specimen.
5. You should be aware of the cleaning procedures to be used after the use of oil. Wipe oil from
the oil immersion objective, and from any other parts in need of cleaning. Clean lenses immediately
with xylene after use. Leaving oil on the lens may lead oil to seep behind and harden on the inside of
the lens.
★To obtain a bright and clear image, it is import to adjust the illumination system. The condenser
position and the iris diaphragm size should match with objectives of different magnifications. Turn the
condenser focus knob to shift the condenser. It needs to raise the condenser when using the high
magnification objective, and to decline when using the low magnification one.
★The aperture diaphragm is used to adjust the numerical aperture instead of brightness.
Generally, setting the size of the aperture diaphragm at 70%～80% of the numerical aperture, you can
obtain a clear image with enough contrast.
★For observing a batch of specimen of the same type, use the limit bolt to locate general
position of the stage to simplify operation. In this way, the specimen can come into focus rapidly just
by turning the coarse focusing knob.
Note: specimens of the same type means they are same in the thickness of slide, cover slip and
specimen slice.
7. Maintenance
1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being damaged.
2. All the lenses have been well checked and adjusted. It is forbidden to disassemble them by user
self.
3. The nosepiece and coarse/fine focus unit have a compact and precise frame, so please don’t

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disassemble them as possibly as you can.
4. Keep the instrument clean, wipe dust regularly, apply a little non-corrosive grease in the sliding
parts and be attention to avoid contaminating the optical elements especially.
5. Place the instrument in a cool, dry position. When not using the microscope, keep it covered with
a dust helmet. Make sure the lamp socket is cool before covering the microscope.
8. Troubleshooting
Under certain conditions, performance of this unit may be adversely affected by factors other
than defects. If a problem occurs, please review the following list and take remedial action as needed.
If you cannot solve the problem after checking the entire list, please contact your local distributor for
assistance.
1) Optical part
Problem
Cause
Solution
The edge of the field of
view has shadow or
the brightness is
asymmetry
The nosepiece is not in the located
position (objective is not in the center of
the optical path)
Turn objective to the right
position (the optical light
center)
The image of the filament is not in the
center
Center it
The surface of the lens is moldy or has
contaminant (include condenser,
objective, eyepiece and collector)
Clean it up
Find dust and stain in
the field of view
There are stains on the lens (include
condenser, objective, eyepiece and
collector)
Clean it up
There are stains on the specimen
Clean it up
The position of the condenser is too low
Raise it to proper position
The image is
defocused (low
resolution \ contrast)
There is no coverslip on the specimen
Add coverslip
The coverslip is too thick or too thin
Use the standard coverslip
( 0.17mm )
The specimen is placed inversely
Reverse it back
There was oil on the dry objective (easily
happened in 40X objective)
Clean it up
There are stains on the lens (including
condenser, objective, eyepiece and
collector)
Clean it up
The oil objective didn’t immersion oil
Use immersion oil
There was bleb in the oil
Eliminate the bleb
Use an unsuitable oil
Change to the specified
one

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The size of the aperture diaphragm is too
large
Minify it
The size of the aperture diaphragm is too
small
Open it up
The position of the condenser is too low
Adjust the position
One side of the image
is dark
The condenser is not in the center of the
field of view\the condenser inclines
Re-install the condenser
The nosepiece is not in the right position
Turning it until it reaches
the “clicked” position
The specimen is floating
Fix it
The image shift during
focusing
The specimen slips on the stage
Fix it
The nosepiece is not in the right position
Turn it to the “clicked”
position
2) Mechanical part
TROUBLE
CAUSE
SOLUTION
The image can focus
when using high
magnification objective
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip
(0.17 mm)
The objective and the
specimen touch when
change objectives from
low magnification to
higher magnification
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip
(0.17 mm)
The specimen is not
easy to move
The specimen holder is not fixed
Fix it
The eyes are too tired
The brightness is not suitable
Adjust the voltage of the
lamp
3) Electrical Part
TROUBLE
CAUSE
SOLUTION
The lamp can’t light
when turning on the
switch
No power supply
Check the connection of
the power cord
The LED lamp is burnt out
Replace it
The lamp burns out
suddenly
The voltage is too high
Use the specified AC
adapter, if the problem is
not solved, contact with
the service department

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The brightness is not
enough
Use a substandard AC adapter
Use the specified AC
adapter
The voltage is too low
Add the voltage
9．The software Scopeimage 9.0
1) Installation
1. Link the digital Binocular head to a PC by the USB cable, the system will find the new device, and then
run the driver under the CD, please follow the clue to finish the installation.
Attention: please link the camera to a PC before running the driver installation; and when there
comes out warning dialog, just click ‘Continue Anyway’, as the pictures show below:
2. Install the software, follow the clue to finish the installation, after the installation, there will be a
shortcut icon on the Desktop and the Start Menu.
3. Please install the video codec ‘wmv9VCMsetup.exe’, it can compress the video files when recording,
and greatly reduce the size of the video files. If you can not do record, please check whether you have
installed the video codec already.
4. Double click the shortcut icon on the desktop to preview the images, please insure the camera has
linked with the PC, and the driver has been installed rightly, or there will come out an error hint:
Cannot preview successfully. Please check your camera.
Attention:
★Please install all the three setup files in the CD, otherwise the camera may can not work normally.
★USB2.0 is a mandatory, not optional. This camera cannot work on the computers with USB1.1 ports.
In order to ensure the stability of the connection with the USB2.0 interfaces, please insert the plug
into those interfaces in the back panel the mainframe.

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★Please use the same USB2.0 port every time. Using on a different USB2.0 port requires reinstalling or
updating the driver again to make the camera work rightly. If not, it will influence the performance of the
camera, and cause the live image displays drag, for there is another system driver, and the system set it as
default driver.
★Support operation system: Windows2000/XP/Vista/Win 7/Win 8/Win 10, 32bit & 64bit operation
system.
★Please unplug the USB cable from the computer immediately after using. It will shorten the lifetime of
the camera, sometimes may cause repair service if the camera is plugged in the computer all the time
and you do not shut down the computer for a long time.
2) Operation
ScopeImage 9.0 is image-processing software professionally designed for digital microscope. It allows you
to view, capture, edit, record, zoom, measure, and process microscope images. The main interface of the
software is as follows:

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Besides image windows, many other windows or user interface elements reside within the main window
of ScopeImage 9.0. These include menu, toolbars, status bar and control windows, providing additional
information about the images or control of the camera.
ScopeImage 9.0 mainly divides into two parts operation, one is for the video operation, and the other one
is for the image operation;
Video operation includes video preview, video control, color adjustment, video capture, ect.
Image operation includes image processing, image flip, image calibration and measurement, ect.
ScopeImage 9.0 is image-processing software professionally designed for digital microscope. It allows you
to view, capture, edit, record, zoom, measure, and process microscope images. Specific features included
are as follows:
1）Active image preview, freezing preview, fit to window preview, real preview, full screen
preview… various preview modes make you preview the active images with great ease.
2）Convenient color adjustment of the active image, containing brightness, contrast, saturation,
RGB value, white balance and so on, very convenient to adjust the color during previewing.
3）Special design color database, we can associate the parameters of the color to the database, and
can store or delete the color scheme.
4）Various image measurement tools, including line, angle, rectangle and circle, can move, delete
or set the color of the objects. And you can also burn all the objects into the image for further
using.
5）Basic image operations like other similar software are included in the Image menu. You can
adjust the image at ease.
6）Image flip function, include horizontal flip and vertical flip of the active image, and rotate 90
degree, 180 degree, 270 degree or any angle degree of the captured image.
7）Image Zoom: Contain image zoom in, zoom out, 1:1.
Attention:
When launching the software, there will be several seconds displays as black preview, please don’t worry,
it is normal, because the camera needs time to do some pretreatment, to ensure the stable working of
the camera. The same reason for the recording, when you begin to record, it will display as black preview
for several seconds, and when the images display normal, the recording begins.
Detail information about how to operate the software, please refer to the user manual in the CD.