BestScope BS-6022RF User manual

Metallurgical Microscope
Model: BS-6022RF/TRF
User Manual
It is recommended strongly that you study this manual thoroughly before using the microscope.
Retain this manual in an easily accessible place near the work desk for future reference.

BestScope International Limited
1
Contents BS-6022RF/TRF
USER NOTICE…………………………………………………………… 2
1. Components………………………………………………………………3
2. Assembly……………………………………………………………………5
3. Operation………………………………………………………………… 10
4. Observation Methods ……………………………………………… 17
5. Specification …………………………………………………………… 19
6. Troubleshooting ……………………………………………………… 20

BestScope International Limited
2
User Notices BS-6022RF/TRF
I. Safety note
1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being
damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy
shaking environment. Make sure the stage is smooth, horizontal and firm enough.
3. When moving the instrument, please use two hands to grip with the two sidesofthe
microscope body.
4. When running, the lamp house and nearby parts will be very hot. Please ensure there is enough
cooling room for them.
5. Always ensure that the grounding terminal of the microscope and that of the wall outlet are
properly connected. If the proper power cord is not used, product safety performance cannot be
warranted.
6. For safety, be sure the main switch is in “O”(off) state before replace the halogen lamp or the
fuse, then cut off the power, and do the operation after the lamp bulb and the lamp house
completely cool.(Specified Lamp: G6.35 24V100W halogen lamp)
7. Use the factory supplied power cord, please.
II. Maintenance
1. All the lenses have been well checked and adjusted. It is forbidden to disassemble them
yourself.
2. The nosepiece and coarse/fine focus unit have a compact and precise frame, please don’t
disassemble them as possible as you can.
3. Keep the instrument clean, wipe dust regularly, and be attention to avoid contaminating the
optical elements especially.
4. The contaminations on the prism, as finger mark and oil, could be gently wiped with a piece of
soft cloth or tissue paper, gauze which has been immersed in pure alcohol or xylene. (note that
the alcohol and the xylene are all burned easily, do not let them near the fire, and use them
in a drafty room as possible as you can.)
5. Don’t use organic solvent to wipe the non-optical elements, when you needtoclean,usethe
soft detergent, please.
6. When using, if the microscope is splash by liquid, cut off the power at once, and wipe up the
moisture.
7. Do not disassemble any parts of the microscope. That will affect the function or decline the
performance of the microscope.
8. Place the instrument in a cool, dry position. After using the microscope, remember to cover it

BestScope International Limited
3
with dust helmet. Do wait for the lamp house cooling completely before cover.
III. Electrical Parameters:
1. Voltage Rating: 100-240V AC, 50/60HZ
2. Power Rating: 200VA
3. Fuse specs.50T T5AL250V
4. Lamp: 24V100W

BestScope International Limited
4
1. Name of Components BS-6022RF/TRF
Components Name of BS-6022TRF
Mirror Selector Lever
Li
g
ht Path Selecto
r
Leve
r
Analyzer Insertion Slot
ND Filter Lever
Polarizer Insertion Slot
Aperture Diaphragm Lever
Field Dia
p
hra
gm
Leve
r
Main Switch
Brightness Adjustment Knob
Right Fine
Focus Knob
Mechanical Stage
Portrait Adjustment Knob
Lateral Adjustment
Knob
Lamp housing
Trinocular Viewing Unit
Filter Insertion Slot (The
filter should be inserted
fro
m
the left
)
Right Coarse
Focus Knob

BestScope International Limited
5
BS-6022RF/TRF
Components Name of BS-6022RF
Trinocular Viewing Unit
Li
g
ht Path Selecto
r
Leve
r
Aperture Diaphragm Lever
Filter Insertion Slot (The
filter should be inserted
fro
m
the left
)
Analyzer Insertion Slot
Lamp housing
Mechanical Stage
ND Filter Lever
Mirror Selector Lever
Field Dia
p
hra
gm
Leve
r
Main Switch
Polarizer Insertion Slot
Lateral Adjustment
Knob Brightness Adjustment KnobPortrait Adjustment
Knob
Right Fine Focus Knob
Right Coarse Focus Knob

BestScope International Limited
6
2．Installation BS-6022RF/TRF
2-1 Installation Diagram
The following figure shows the installation sequence of the components take BS-6022TRF for
example. The number in the figure show the installation steps.
Before installing, be sure every components is clean, do not score any parts or glass surface.
Keep well with the supplied hexagon wrench. When changing the components, you will need
it again.
Video Port
Trinocular Viewing Unit
10 ×Wide Field
Eyepiece
Lamp
housing
Reflected Light Brightfield/Darkfield
Illuminator
Infinite Plan Objective
Mechanical Stage Support
Device Microscope Body
①
⑤
⑥
③
⑦
④
②
⑧
Lamp
housing

BestScope International Limited
7
2-2 Installation Steps BS-6022RF/TRF
2-2-1 Installing the Mechanical Stage
Support Device
1. Before installing the device, be sure to adjust
the coarse focus knob ①.Maketheguide
board ②down to the lowest position, so you
can install the mechanical stage support
device easily.
2. Hold on the mechanical stage support device
(Fig. 2), place it from the top of the guide
board (Fig.1), let the device (Fig.2) falling
free until it reach the limit bolt. Use the
hexagon wrench screw down the locking
block③, make the stage support device and
the guide board fixed together(Fig.3).
Fig.1, Fig.2 show the installation sequence
of the components which take
BS-6022TRF for example. For
BS-6022RF, there are two limit bolt
holes with different height on the guide
board ②to meet with sample which
have different thickness: the upside hole
is for sample with 0 ～30mm thickness,
the underside hole is for sample with
20～50mm thickness.
The mechanical stage ⑤have been
adjusted horizontally and fixed together
before leaving factory. Do not
disassembly unless necessary, that may
affect the observation precision of the
instrument.
Fig.1
②
①
Fig.2
⑤③
④
limit
bolt

BestScope International Limited
8
BS-6022RF/TRF
2-2-2 Installing the reflected light
brightfield/darkfield illuminator
1. Installing the reflected light illuminator
(Fig.4) on the head of
microscope body(Fig. 5), and turn to a
proper position, then screw down the bolt
⑥to fix it.
2-2-3 Installing the Video Port
1. Insert the video port (Fig.6) into the
illuminator (Fig. 4), then screw down
the bolt to fix it.
2-2-4 Installing the Objective
1. Adjusting the coarse focus knob until the
support device of the mechanical stage
reach its low limit position.
2. Wresting the lowest magnification
objective onto the nosepiece from the
left or the right side, then push the
nosepiece clockwise, then place other
objectives by the sequence of low to
high magnification (Fig.7).
Installing objective this way will make
the change of magnification to be easier
while in using.
Clean the objective regularly, the
objective of the inversed microscope
is very sensitive to dust.
When replacing the objective, slowly
turning the nosepiece until you hear
“clicked”, that means the objective
enter the required position--the light
path center.
Fig.4
⑦
⑧
Fig.6
⑨
Fig.7

BestScope International Limited
9
BS-6022RF/TRF
2-2-5 Installing the Video Port (optional)
Insert the video port (Fig. 8) into the trinocular
unit (Fig. 6), then screw down the bolt ⑨to
fix it.
2-2-6 Installing the Eyepiece
Insert the eyepiece (Fig.9) into the eyepiece
tube until they are against each other.
2-2-7 Installing the Lamp Housing
1. Pushing the lamp holder (Fig.10)into the
illumination kits(Fig. 4) gently until they are
against each other, turn to a proper position, then
screw down the bolt ⑧to fix it.
2. Insert the power plug①into the power jack of
the microscope②, and screw down the bolt to fix
it. The result in show in Fig. 12.
◎When using transmitted light illuminator,
remove the dust cap first, then pushing the lamp
holder (Fig.10)into the jack on the back of the
microscope (Fig.11) gently and turn to a proper
position, then screw down the bolt ④. Insert the
power plug ①into the power jack of the
microscope③, and screw down the bolt to fix it.
Fig.8
Fig.9
Fig.10
①
Fig.11
②
③
④
Fig.12

BestScope International Limited
10
BS-6022RF/TRF
Fig.13
①
②
Fig.14
③④
Fig.15
2-2-8 Attaching the Halogen Bulb
The applicable lamp bulb is the 24V100WHAL.
1. Fully loosen the clamping screw ①at the
top of the lamp housing using the provided
hexagon wrench.
2. Remove the lamp housing ②by lifting it
up.
3. Tilt the bulb socket by 90°(Fig. 14)
4. While pushing down the bulb clamping
levers, hold the halogen bulb with gloves or a
piece of gauze, insert the bulb pins into
the sections as far as they will go.
Then return the lamp clamping lever gently
back to the original position to clamp the bulb.
Caution for Bulb Replacement Durig or
Right After Use
When using, or soon after it is turned off the
lamp, the lamp house and nearby parts will be
very hot and will cause serious burns. Please
turn the main switch on “O” (off), pull out
power plug, and make sure the bulb, the lamp
room and periphery are all cool. Then, you can
do your replacing.
Please insert the lamp gently, or it will be
damaged by excessive extrusion.
DonottouchtheHalogenbulbwithyour
bare hands. It will shorten the service life
or cause it to burst. If you leave finger
marks on the surface carelessly, clean it
with a dry soft cloth.
When using, the temperature of the
lamp housing surface will be very hot,
please pay attention to the warning
written on the Warning board.

BestScope International Limited
11
3. Operation BS-6022RF/TRF
3-1 Turning on the Lamp
Connect the power, press the main switch
①to the “I”(on) position.
◎When using transmitted light illumination,
fixing the lamp holder into the jack on the back
of the microscope (Fig.11) ,and insert the power
plug into the power jack of the microscope (③in
Fig.11).Then press the main switch ①to the
“‖”(on) position.
3-2 Adjust Brightness
Turning the brightness adjustment knob ②
clockwise, the voltage raise, and the
brightness strengthen; turning with the
anti-direction, the voltage decline, and the
brightness weaken.
Using the lamp in a low voltage condition,
will prolong the use life.
3-3 Adjust the Tension Adjustment
Collar with Spanner
The tightness of the tension adjustment
collar has adjusted before leaving factory, if
finding it’s loosing (the mechanical stage
drop itself because of deadweight), please
turning the tension adjustment collar ③
until the tightness is in order.
Fig.16
①②
③
Fig.17
spanner

BestScope International Limited
12
BS-6022RF/TRF
3-4 Placing Specimen
1. Fix the specimen ②on the sample board ①
by plasticine ③,besurethatthespecimen
surface is parallel to the sample board . It is
conveniently to use smoothing presses to
flatten the specimen（Fig. 19）
2. Place the slide on the mechanical stage. Use the
stage clips to clamp the slide gently.
3. Turn the portrait and lateral adjustment knob of
the mechanical ruler, move the specimen onto the
required position.
Be careful when changing the objective. If
you finish the observation with the short working
distance objective, and want to change another r
one, be careful of not letting the objective touch
the specimen.
3-5 Adjusting the Inter-pupillar Distance
The inter-pupillar distance range: 48mm ～
75mm. When observing with two eyes, hold on
the left and right prism holder, turn around the
axis(Fig.28), adjust the inter-pupillar distance
until the left and right fields of view coincide
completely.
3-6 Adjusting the Diopter
The right ocular tube is fixed. So by turning
the left diopter ring after the right ocular focus on
the specimen, the operator who’s left and right
eye has different eyesight can obtain a
comfortable focus position with both eyes.
图20
Fig.20
图18
Fig.18
①
②
③
Fig.19
Smoothing
presses
Diopter
adjustment ring
Fig.21

BestScope International Limited
13
BS-6022RF/TRF
3-7 Focus
When not using the video set
1. Push in the light path selector lever ①
completely, then observe with both eyes. Use the
10×objective focus, to avoid the objective touch
with the specimen, you should raise the
mechanical stage at first, let the specimen close
to the objective, then slowly separating them to
focus.
2. The operator can converse turn the coarse
focus knob to get the specimen down ,and search
images in the 10×ocular simultaneously, then use
the fine knob to focus. At this moment, you can
replace other magnification objectives safely, and
focus without the risk of destroying the
specimen.
When using the video set
Pull out the light path selector lever ①,
observe with both eyes, when the image is sharp,
you can see the pictures directly on the video
screen which connected by the microphotograph
system through the video mount.
If you need to fix the stage on a vertical
position to make the observation become
more convenience, take use of the locking
set②.
①
Fig.22
Fig.23
②

BestScope International Limited
14
BS-6022RF/TRF
Fig.24
Fig.26
②
④
Usable Filters Applications
Color temperature
conversion filter
Turns the illumination light into daylight.
Used in general observations and color
photography.
Green filter Enhanced contrast in monochrome
observation. Used in monochrome
photography
Yellow filter Contrast filter for observation of
semiconductor wafers
Frost filter Reduces irregularity in the illumination
field, but also reduces the brightness
ND25 Adjusts the brightness of the light source.
(Transmittance: 25%)
ND6 Adjusts the brightness of the light source.
(Transmittance: 6%
3-8 Usin
g
the ND Filter Knob
1. The ND Filter is interlocked with brightfield
(BF) light path switching so it can be engaged
or disengaged according to the mirror selector
lever ①.The ND filter makes it possible to
reduce the glare when darkfield(DF)is switch
to brightfield(BF).
Releasing interlocking
◎The ND filter knob has been interlocked at the
factory. If brightness is not enough during
brightfield, DIC or other observation, the
interlocking can be released.
1. Loosen the screw interlocking the ND filter by
inserting the Allen screwdriver into the hole
②on the left side of the reflected light
illuminator.
2. Now the interlocking is released and the ND
filter knob (Fig. 17) is active. Pull the lever
out to disengage the ND filter from the light
path.
3-9 Usin
g
the Filters
◎Engage the optimum filter sliders for the
purpose of observation in the two filter
insertion slots ④. Be sure to engage from the
left side.
◎The first click position is the idle position and
the second click engages the filter in the light
path.
Fig.25
Fig.25

BestScope International Limited
15
BS-6022RF/TRF
3-10 Selecting the Light Path of the
trinocular Tube
1. Slide the mirror selector lever ①toward the
indication of the mirror for the desired
observation method.
BF: Reflected light brightfield observation
DF: Reflected light darkfield observation
◎Be sure to slide the mirror selector lever
until it contacts the stopper.
3-11 Centering the Field Iris
Diaphragm (FS)
1. Slide the mirror selector lever ①to “BF”.
2. Engage the 10X objective by rotating the
revolving nosepiece, place the specimen on
the stage and adjust approximate focusing.
3. Pull out the FS knob ④on the reflected
light illuminator to reduce the aperture iris
diaphragm a little.
4. Rotate the two FS centering screws②using
the Allen screwdriver to adjust so that the
field iris image becomes concentric with
the field of view.
5. While pushing in the FS knob ④,openthe
field iris diaphragm until the field iris
image inscribes the field of view. If the
image is found to be eccentric, adjust the
centering again.
6. Open the field iris so that its image is almost
the same size as (Fig. 28 subscribes) the
field of view.
Using the Field Iris Diaphragm
●In reflected light brightfield observation
The field iris diaphragm adjusts the illuminated
area to obtain an image with high contrast.
According to the objective in use, adjust the FS
knob ④of the reflected light illuminator until
the iris image circumscribes the field of view to
block unnecessary light.
●In reflected light darkfield observation
The field iris diaphragm must be opened by
pushing in the FS knob.
Fig.28
Field iris diaphragm image
Field o
f
view of e
y
e
p
iece
①
②③
④
⑤
Fig.27

BestScope International Limited
16
BS-6022RF/TRF
3-12 Centering the Aperture Iris Diaphragm
(AS)
1. Slide the mirror selector lever to “BF”.
2. Engage the 10X objective by rotating the
revolving nosepiece, place the specimen on
the stage and adjust approximate focusing.
3. Remove the eyepiece, look into the eyepiece
sleeve and pull the AS knob ⑤,sothatthe
aperture is about 70%.
4. If the center of the iris diaphragm is deviated,
center it by rotating the two
AS centering screws( ③in Fig. 27) using the
Allen screwdriver.
Using the Aperture Iris Diaphragm
●In reflected light brightfield observation,
optimum observation is generally possible by
settingtheaperturetobetween70%and80%of
the aperture number of the objective(figure 29).
●In reflected light darkfield observation,the
aperture must be fully opened by pushing in the
AS knob.
◎With some specimens, an image with high
contrast and little flare may sometimes be
obtained when the aperture is slightly closed. It
is therefore recommended to also try a slightly
closed aperture.
3-13 Setting the Analyzer and Polarizer
1. Insert the polarizer(Fig. 30) into the polarizer
Insertion Slot ③with the surface printing with
Silk Screen towards you, then push the polarizer
into the light path.
2. Remove the cover, then put the analyzer
(Fig.30 in the insertion slot(②in Fig.31).
3. Rotate the analyzer rotating dial ①to find the
position where the field of view is darkest.
4. When the analyzer and polarizer are coupled
by using the coupling plate | provided with
the polarizer and tightening the clamping
knobs on it, the analyzer and polarizer can be
engaged or disengaged in the light path
together（Fig.31）.
Fig.29
The image of aperture iris diaphragm
①
Fig.30
Polarize
r
Coupling plate
Analyzer
Fig.31
②
④
③

BestScope International Limited
17
BS-6022RF∕TR
3-14 Adjusting the Swing out Condenser (Fig.
32)
Thecenterofthecondenserandthelightaxesof
the objective are coaxial. It has been adjusted
before leaving factory, so the user needn’t to adjust
them by self.
The highest position of the condenser has been
adjusted too. It also needn’t any user’s operation.
Turn the condenser focus knob to shift the
condenser. It needs to raise the condenser when
using the high magnification objective, and to
decline when using the low magnification one.
1. Using the Swing out Condenser
When using the low magnification objective, turn
out the condenser, and let it away from the light
path. While using the high magnification
objective, turn it into the light path.
2. Adjusting the Aperture Diaphragm
The aperture diaphragm is designed for the
adjustment of the numerical aperture , not for the
brightness. Generally, reducing the diaphragm
opening to 70- 80% of the N.A. value of the
respective objective will provide an image of
acceptable quality. If you want to observe the
image of the aperture diaphragm, remove one
eyepiece and look through the tube. You will see a
dark circle encroaching on the bottom of the tube.
3-15 Adjusting the Field Diaphragm (Fig.
33)
The control for the field diaphragm is a ring used
for adjusting the area of field diaphragm. When
using, turn the ring to reduce the field diaphragm,
look into the field, if the diaphragm image is
faintness, do the follow steps: first, turn the
condenser focus knob, shift the condenser holder
to the position where the observed image of the
field of view is sharp; then open the field
diaphragm, let the image full of the field of view,
reduce the mixed light, improving the quality of
the image.
Fig.32
Fig.33

BestScope International Limited
18
Page
－－－－
－－－－
－－－－－－－－－－－－－
－－－－－－－－－－－－－
－－－－－－－－－－－－－
－－－－－－－－－－－－－
－－－－－－－－－－－－－
－－－－－－－－－－－－－
－－－－－
－－－－
－－－－－
－－－－－－
－－－－－－－－－－－－－
ND filter knob (P. 13)
Light path selector knob (P. 14)
Main switch (P. 10)
Stage plate (P. 11)
Revolving nosepiece
Coarse/fine adjustment knobs (P. 12)
Binocular tube (P. 11)
Diopter adjustment ring (P. 11)
AS knob (P. 15)
FS knob (P. 14)
Revolving nosepiece
Coarse/fine adjustment knobs
Brightness adjustment knob (P. 10)
Brightness adjustment knob (P. 10)
4. Observation Methods BS-6022RF/TRF
4-1 Reflected Light Brightfield/Darkfield Observation
◎The following flow shows the basic operating procedure for reflected light brightfield or darkfield
observation.
(Controls Used)
Select the brightfield (BF) or darkfield (DF) observation. －－－－－Mirror selector lever (P. 14)
Set the main switch to “ I ”
(
ON
)
.
Check interlockin
g
of the ND filte
r
Disengage the analyzer, polarizer,
filter
,
etc. fro
m
the li
g
ht
p
ath.
Select the light path
Engage the desired objective in the light
p
ath and
b
rin
g
the s
p
ecimen in focus.
Place the specimen on the stage.
En
g
a
g
ethe10Xob
j
ective in the li
g
ht
p
ath.
Brin
g
the s
p
ecimen in focus.
Adjust the interpupillary distance.
Ad
j
ust the dio
p
ter.
Ad
j
ust the
b
ri
g
htness.
Insert the required filters
Adjust the aperture iris diaphragm and
field iris diaphragm.
◎Open both iris diaphragms in case of
DF observation.
Ad
j
ust the
b
ri
g
htness.
Start observation.
Filter insertion slot (P. 13)

BestScope International Limited
19
BS-6022RF/TRF
4-2 Reflected Light Simplified Polarized Light Observation
1. Setting the Analyzer and Polarizer (Fig. 15).
2. Place the specimen on the stage and adjust the focus by moving the stage up or down.
Now simplified polarized light
Observation can be started.
3. Adjust the field iris diaphragm so that its image circumscribes the fieldofview.
4. The contrast may sometimes be enhanced by closing the aperture iris diaphragm slightly.
4-3 Transmitted Light Observation
1. Installing the lamp housing 。（ see page 8 :Installing the lamp housing when using
transmitted light illuminator）.
2. Installing objective.
3. Installing the swing out condenser.
4. Set the main switch to “Ⅱ” (ON).
5. Fix the specimen on the stage, adjust the focus to begin observation.
◎At the Transmitted Light Observation, the method of condenser ,field iris diagram
adjusting please refer to section 3-14 and 3-15.
4-4 DIC Observation (Difference Interference Contrast Observation)
1. Setting the Nomarski slider
Loosen the mounting knob ①on the front of the DIC revolving nosepiece, insert the
DIC slider②and clamp by tightening the mounting knob.
2. Observation method
2.1 Place the specimen on the stage and adjust the focus by moving the stage upor
down.
2.2 Adjust the field iris diaphragm so that its image circumscribes in the field of view.
①
②
③
3. Adjust the DIC attachment
3.1 Adjust the background contrast by turning the
slider movement knob ③on the DIC prism as
described below.
3.2 When the slider movement knob on the DIC prism
is turned, the interference color at the background
varies continuously from the gray sensitive color
to magenta sensitive color (from -100 to 600 nm).
Set the interference color which can provide best
contrast with respect to the specimen.
Selecting a dark background color enables dark field-type observation.
Selecting a gray background color enables 3D-looking observation with high
contrast thanks to the highest sensibility of the gray sensitive color.
Selecting a magenta sensitive color allows even small phase variation to beobservedas
a change in color.
*Since the DIC observation has a high detection sensitivity, take care against
contamination on the specimen surface.