BestScope BS-4000A User manual

Industry Inspection Microscope
Model Number
BS-4000A/B
Instruction Manual
This manual is written for Industry Inspecting Microscope of BS-4000A/B. For safety and for exerting the best
performance, making you familiar with the instrument entirely, it is strongly recommended that you read this
manual carefully before using the microscope.

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Contents BS-4000A/B
User Notices
3
1
Name of Components
4
2
Installation
5
2-1 Installing steps
5
3
Adjustment & Operation
10
3-1 Adjustment Set Diagram
10
3-2 Operation
11
3-3 Microscope Video
15
3-4 Microscope Photography
16
4
Technical Specifications
17
5
Trouble Shooting
18

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Use Notices BS-4000A/B
I. Safety note
1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy shaking
environment. Make sure the stage is smooth, horizontal and firm enough.
3. When moving the instrument, grip two sides of the bottom of the microscope with your two hands.
4. When running, the lamp house and nearby parts will be very hot. Please ensure there is enough cooling
room for them.
5. Make sure the instrument is earthed, to avoid lighting strike.
6. For safety, be sure the main switch is in “O”(off) state before replace the halogen lamp or the fuse, then
cut off the power, and do the operation after the lamp bulb and the lamp house completely
cool.(Designated bulb: 6V/20W Halogen Lamp)
7. Check the input voltage: be sure the input voltage which signed in the back of the microscope is
consistent with the power supply voltage, or it will bring a serious damage to the instrument.
II. Maintenance
1. All the lenses have been well checked and adjusted. It is forbidden to disassemble them yourself.
2. The nosepiece and coarse/fine focus unit have compact and precise frame, please don’t disassemble
them as possible as you can.
3. Keep the instrument clean, wipe dust regularly, and be attention to avoid contaminating the optical
elements especially.
4. The contaminations on the prism, as finger mark and oil, could be gently wiped with a piece of soft cloth
or tissue paper, gauze which has been immersed in pure alcohol or ether. (Note that the alcohol and the
ether are all burned easily, do not let them near the fire, and use them in a drafty room as possible as
you can.)
5. Don’t use organic solvent to wipe the non-optical elements, when you need to clean, use the soft
detergent, please.
6. When using, if the microscope is splash by liquid, cut off the power at once, and wipe up the moisture.
7. Do not disassemble any parts of the microscope. That will affect the function or decline the
performance of the microscope.
8. Place the instrument in a cool, dry position. After using the microscope, remember to cover it with dust
helmet. Do wait for the lamp house cooling completely before cover.
！Make sure that the input voltage is consistent with the power supply voltage, or it
will bring a serious damage to the instrument.

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1. Name of Components BS-4000A/B
Filter Board
Metallurgical
Illumination System
Eyepiece
Nosepiece
Body
Trinocular Viewing
Head (Fixed)
Stage
Objective

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2．Installation BS-4000A/B
2.1 Installing Steps
Fig.1
Fig.2
①
②
③
2.1.1 Installing the Stage (Fig.1-2)
1. Loosen the setscrew①.
2. Push the stage into the holddown groove
②and make the stage touch the retainer③.
3. Tighten the setscrew① until the stage is
installed firmly.
4. Place glass stage in the center of the
mechanical plain stage.
5. Fig.2 shows the station after Installing the
Stage.

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2.1.2 Installing the Objective (Fig.3-4)
1. Adjust the coarse focus knob①, till the mechanical
stage to the low limited place.
For ensuring the safety of the instrument during
transportation, the nosepiece is located in the
lowest position and the tension adjustment
collar②is adjusted to an appropriate tension
while leaving the factory.
2. Remove the dust cap of the nosepiece.
3. Screw down the objective to nosepiece from left or
right side, low magnification objective first. Install
all the objective form low to high magnification
following the clock hand.
According to this way to install the objective will
make it easier to change magnification in
operation.
Clean the objective frequently.
At first, use the 10X objective to looking for image,
then change another one.
Turn the objective till hear the “Kai. Kai” sound,
make sure the objective enter the objective
center.
2.1.3 Installing the Eyepiece (Fig.5)
1. Remove the cap of the eyepiece tube.
2. Insert the eyepiece into eyepiece tube until they
are against each other.
Fig.3
①
②
Fig.4
Fig.5

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BS-4000A/B
Fig.9
Fig. 10
2.1.4 Mounting the video (photography) adapter
(Fig.6-8)
Insert the video (photography) adapter tube (Fig.6)
into the trinocular viewing head (Fig.7), and screw
down the bolt to fix it, as shown in Fig.8.
2.1.5 Mounting Filters and Polarizer (Fig.9-10)
Insert the filter into the corresponding jack, as shown
in Fig.10.
For polarization observation, insert the polarizer into
the right jack.
★Standard filters include blue, yellow and green filters.
Fig.6
Fig.7
Fig.8

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Fig.13
BS-4000A/B
Fig.12
Fig.14
①
②
⑥
⑦
①
Fig.11
2.1.6 Mounting Analyzer (Fig.11)
Insert the analyzer①into the jack under the
trinocular viewing head if necessary, as shown in
Fig.11.
★Analyzer can be used alone or matched with the
polarizer.
2.1.7 Connecting the Power Cord (Fig.12-14)
★Do not force on the Power Cord. The cable and wire
are easier to be damaged when bended or wrapped.
1. Before connecting the power cord, switch the main
On-Off ① to “O”(off).
2. Plug the power cord into the socket ②on microscope
safely. Make sure be connected.
3. Plug the power cord④ into the power source
socket⑤ safely. Make sure be connected.
★Do use the supplied power cord all the time. If lost
or damaged, select the same standard cord, please.
2.1.8 Replacing the fuse (Fig.12-13)
Do remember to turn the main switch① on the state
of “O” (off) before replacing the fuse, and unplug the
power cord. Rotate the fuse kits ⑥ out of the
holder⑦ by the “--”type screwdriver, replace a new
fuse, then rotate back to the holder again.

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Fig.16
Fig.17
2.1.9 Installing and replacing the lamp (Fig.15-18)
★Please use the specified halogen Lamp 6V20W.
1. Pull out the lamp-house① as the picture show (Fig.15).
2. Hold to the bulb ①after you wrap it with gauze or
other protection materials, then depress the plugs
②fully into the jack ③ on the lamp house as the
picture show. (Fig.16)
3. Replacing the lamp when using or soon after
When using, or soon after it is turned off, the lamp, the
lamp house and nearby parts will be very hot and will
cause serious burns. Please turn the main switch on
“O” (off), pull up power plug, and make sure the bulb,
the lamp house and periphery are all cool. Then, you
can do your replacing.
Please insert the lamp gently, or it will be damaged by
excessive extrusion.
Do not touch the Halogen bulb with your hands. It will
shorten the service life or cause it to burst. If you leave
fingerprints on the surface carelessly, clean it with a dry
soft cloth.
4. Align the socket① and pins ③and align the bolt②
and the jack④. Then push the lamp-house into the
Illumination Set until covered. (Fig.17-18)
Fig.15

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3. Adjustment & Operation BS-4000A/B
3.1 Adjustment Set Diagram (Fig.19)
-+
Coarse
Focus Knob
Fine
Focus Knob
Portrait Adjustment
Knob
Lateral Adjustment
Knob
Interpupillary
Distance
Diopter Ring
Light Switch
View field
Diaphragm
Aperture
Diaphragm
Gather Lens
Adjust Lever
Stage moving Knob
Fig.19

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3.2 Operation
3.2.1 Open Lamp-House (Fig.20)
Connect the power, turn on the main switch①
(Fig.20) to “-”(on).
3.2.2 Adjusting the Brightness (Fig.21)
Turning the brightness adjustment knob①
anti-clockwise, the voltage raise, and the brightness
strengthen. Turning it clockwise, the voltage decline,
and the brightness weaken.
★Using the lamp in a low voltage condition, will
prolong the use life.
3.2.3 Adjusting the Illumination Set (Fig.22)
1. Make sure the ray brightness in view field is even,
have no filament shadow. If there is filament shadow,
please adjust the Condenser Knob③ to proper
position. Insert the Ground Glass into the
corresponding jack when using the 4×objective.
2. Adjust the View field Diaphragm① and Aperture
Diaphragm② until the two Diaphragm open size is
properly. Then you can get clear image. When in
using, you need to adjust and observe at the same
time until in best state.
★Aperture Diaphragm: The aperture diaphragm (iris
diaphragm) is designed for matching the
objective's numerical aperture, not use for
adjusting brightness. When the objective is
adjusted full of light, the Aperture Diaphragm is in
best state and the image is clear. What to be notice
is that when switch objective, the Aperture
Diaphragm size should change along with the
adjusting.
★View field Diaphragm: Used to control view field
size, to reduce Anti- cast light and Dazzled light.
When the objective is adjusted full of light, the
image is clear and in high-point state.
Fig.20
Fig.22
Fig.21
①
①
②
③
①

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3.2.4 Adjusting the Diopter (Fig.23)
1. Observe the right ocular tube with your right eye.
Turn the Coarse & Fine Focus Knob to focus the
specimen.
2. Observe the left ocular tube with your left eye. If
not in focus just adjust the Diopter Ring① to
make it in focus.
★ the range of Diopter Ring is ±5, as the value align
the reticle of the ring.
3.2.5 Adjusting the Interpupillar Distance
(Fig.24)
When observing with two eyes, hold on the left
and right prism holder②、③, turn around the
axis, adjust the interpupillar distance until the left
and right fields of view coincide completely.
3.2.6 Focusing (Fig.25)
1. Use the 10×objective focus, to avoid the objective
touch with the specimen, you should raise the
mechanical stage at first, let the specimen close to
the objective, then slowly separating them to
focus.
The operator can converse turn the coarse focus
knob① to get the specimen down ,and search
images in the 10×ocular simultaneously, then use
the fine knob② to focus. At this moment, you
can replace other magnification objectives safely,
and focus without the risk of destroying the
specimen.
If you need to fix the stage on a vertical position to
make the observation become more convenience,
take use of the locking set③.
Fig.23
Fig.24
Fig.25
①
②
③
①
②
③

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3.2.7 Placing Specimen (Fig.26-27)
1. Place the slide on the center of the mechanical
stage①.
2. Turn the lateral②and portrait③adjustment knob of
the mechanical ruler or the mechanical stage moving
knob④ to make the specimen onto the required
position.
Be careful when changing the objective. If you finish
the observation with the short working distance
objective, and want to change another one, be
careful of not letting the objective touch the
specimen.
3.2.8 Adjusting the Tension Adjustment Collar
(Fig.28)
The tightness of the tension adjustment collar has
adjusted before leaving factory, if finding it’s loosing
(the mechanical stage drop itself because of
deadweight), please turning the tension adjustment
collar① until the tightness is in order. Turn it along
the direction show in the picture, the Coarse Focus
Knob② will become tighter. Turn anti-direction will
become loosen.
If the mechanical stage drop itself, or even lose focus
just after adjusting the fine focus knob③. Mean the
tightness of the Coarse Focus Knob② is too low. You
should turn the tension adjustment collar along the
direction show in the picture to make it tighter.
Fig.26
Fig.28
②
③
①
①
②
③
Fig.27
④

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BS-4000A/B
Light Path Selecting Lever
Brightness Proportion
Application
Pushed in
100% used for binocular observation
Binocular observation
Pulled out
20% used for binocular observation,
and 80% used for video or photography
Binocular observation and television
\micrography \ video can be
performed simultaneously.
3.2.9 Switching the Light Path (Fig.29)
◎Slide the light path selector lever① by your
thumb to select the light path you need.
◎For the binocular observation, push in the lever
until you hear “clicked” .while for video or
photography, pull out the lever until it reaches
the “clicked” position.
3.2.10 Polarization Observation
1. Mounting the polarizer and analyzer, the
detail is available in section 2.1.5, 2.1.6.
2. The analyzer hand wheel can be rolled
from 0°to 90°. When the field of view
becomes the darkest, the orthogonal
polarization position is reached and
polarization observation can be
performed.
Fig.29
①

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Microscope Video and Photography BS-4000A/B
3.3 Microscope Video
3.3.1 Selecting the Light Path (Fig.30)
★Just used in the trinocular viewing tube.
Pull out the light path selecting lever, until you hear
the “clicked”.
★For the observation of dark specimen, you can focus
it through the binocular at first, then change the
light path.
3.3.2 Installing the Video Set (Fig.31)
1. Loosen the locking bolt① on the trinocular viewing
tube, and take out the dust cap②.
2. Remove the dust cover on the both ends of the video
accessories③, and revolve the CCD/CMOS set into
the screw thread end---the video adapter with C
mount.
3. Install the video accessories ③into the tri-through
port and screw down the bolt①.
3.3.3 Focus (Fig.31)
Looking through binocular, focus the specimen to get
a sharp image, and then check the image on the TV
or the computer which is connected with the
microscope video set. If it is not clear, please revolve
video adapter③until the image is sharp enough.
Fig.30
Fig.31
①
1
CCD
Video
2
3

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3.4 Microscope Photography
3.4.1 Selecting the Light Path
★Just used in the trinocular viewing tube.
The detail is available in section 3.3.1.
3.4.2Installing the Photography Set (Fig.32)
1. Loosen the locking bolt① on the trinocular viewing tube,
and take out the dust cap②.
2. Install the photography accessories③ into the tri-through
port, and screw down the locking bolts①.
3. Couple the digital photography adapter with the camera set
and insert the adapter into the accessories③.
● Before connecting the camera set with the digital
photography adapter, please remove the camera gun firstly.
Pay attention to the camera port type, please.
● To avoid disturbing from the binocular, please place the
viewfinder toward the side of the microscope when
installing the camera set.
● The magnification of photomicrograph = magnification of
objective ×magnification of photography adapter.
★When shooting the micrograph, the shutter release will
bring some impact. In order to weaken the impact and
obtain a clear image, you could select a longer time of
exposure or decrease the brightness to have some
compensation.
★This explanation is used for NiKon single-lens reflex digital
camera.
3.4.3 Focus
Do the binocular observation and focus the specimen firstly.
When in microscope photography, do use the camera
viewfinder to focus the specimen. Please refer to the user
manual of the photo attachment to obtain the details.
3.4.4 Adjusting the Color Temperature
◎When shooting the chromo photograph with the sunlight
film:
1. Mount the blue filter into the filter bracket.
2. Turn the brightness adjustment knob to the maximal limit
and you can obtain a sunlight illumination.
Fig.32
1
2
3
Camera
Digital photography adapter

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4．Technical Specifications BS-4000A/B
1. Main specifications
Optical System
Infinite Optical System
Viewing Head
Compensation Free Trinocular Head, Inclined at 30
Eyepiece
Extra Wide Field Eyepiece EW 10X/22
Nosepiece
Quadruple Nosepiece
Objective
Infinite plan Achromatic: 4×, 10×, 20×, 40×
Focusing System
Coaxial Coarse and Fine Focusing System, with a distance of 24mm
Stage
area: 300×268mm, movement range: 250×250mm
Lamp-House
Halogen Lamp 6V20W, Intensity Continuously Adjustable
Color Filter
Blue, Yellow, Green and Ground Glass
2. Objectives Specification
Magnification
Numerical Value Aperture
Diaphragm (N.A)
Working Distance
(mm)
4X
0.10
17.3
5X (optional)
0.12
15.5
10X
0.25
10.0
20X
0.40
5.8
40X
0.60
2.9
50X (optional)
0.75
0.32
100X (optional)
0.80
2.0

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5. Trouble shooting BS-4000A/B
PROBLEMS
REASON FOR PROBLEMS
SOLUTION
I. Optical Part:
1. Illumination is opening, but
the field of view is dark.
The poor contact exists in the lamp house and
the illumination system.
Ensure the contact pin and the lamp holder pin
work well
The objective is not in the center of the light
path.
Turn the nosepiece to the located position
The lamp bulb spoils
Change a new bulb
The brightness adjustment knob is set too dark
Adjust the knob in a proper position
No use the appointed lamp bulb
Use the specified halogen Lamp 6V20W
2. The edge of the field of
view has shadow or the
brightness is asymmetry
The nosepiece is not in the located position
Adjust it into the located position
The surface of the lamp become black
Change a new lamp bulb
The filament shadow not clean up
Move in Condenser adjust knob front and back
to change the focus position to clean up the
filament shadow.
The surface of the lens is moldy or has
contaminant
Clean the lens
3. Find dust and stain in the
field of view
There are stains on the specimen
Change the specimen
There are stains on the eyepiece
Clean the eyepiece
4. The image is defocus,
low-resolution
The objective damage
Mend and correct the objective (send to
factory for overhauling)
The lens of the objective and eyepiece is moldy
or have contaminant
Do cleaning
The opening of Aperture diaphragm and field
diaphragm is not proper, and too much
astigmatism.
Change the opening of the aperture diaphragm
and field diaphragm
Fine focus system is broken
Examine and repair the fine focus system(send
to factory for overhauling)
The objective is not in the center of the light
path
Turn the nosepiece to the located position
5. The image focus surface
incline(one side is clear and
the other side is faint)
The illumination light incline serious
Adjust the filament position ,let the light
distributing of the field of view become
symmetrical and bright
The specimen is not placed in required position
Put the specimen on the right position
The nosepiece is not in the located position
Turn the nosepiece in the required position
6. The eyes are
uncomfortable, the left and
right fields of view is not
coincided.
The interpupillar distance is not correct
Adjust the interpupillary distance correctly
The diopter is not right
Adjust the diopter according your sight
Can’t adapt to binocular observation
When look into the objective, do not stare at
the specimen but at the whole field of view, or
move the eyes away to see other things, then
back into the objective

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BS-4000A/B
II. Mechanical Part:
1. The coarse focus knob is
hard to run
The tension adjustment collar is too tight
Loose properly
2. The image can’t stay on
the focal plane in the process
of the observation
The tension adjustment collar is too loose
Tighten properly
III. Electric Part:
1. The lamp can’t light
No power supply
Check the power cord, and connect them exactly
The installation of the bulb is wrong
Install the bulb correctly
The bulb burn out
Change a new bulb
2. The bulb burn out in a high
frequency
Not use the specified lamp
Use the required lamp
3. The height of the
brightness is not enough
Not use a appointed lamp
Use a appointed lamp
The brightness adjustment knob is used
wrong
Adjust the brightness adjustment knob in a correct
way
4. The light glimpse
The plugboard burn out
Change a new plugboard(send to factory for
overhauling)
The power cord have a poor contact
Check the power cord, and connect them exactly