Biacore T100 User manual

Instrument Handbook
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Biacore T100

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BiacoreT100
Instrument Handbook
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_________________________________________________________________________Contents
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Contents
1. Introduction 1
NKN _á~ÅçêÉ∆=qNMM=ëóëíÉã= N
NKO qÜÉ=fåëíêìãÉåí=e~åÇÄççâ= O
NKP ^ëëçÅá~íÉÇ=ÇçÅìãÉåí~íáçå= O
NKQ p~ÑÉíó=áåÑçêã~íáçå= O
NKQKN o~íáåÖ=áåÑçêã~íáçå= O
NKQKO p~ÑÉíó=ä~ÄÉäë= P
NKQKP p~ÑÉíó=áåëíêìÅíáçåë= Q
NKQKQ aáëéçë~ä=éêçÅÉÇìêÉë= R
NKQKR bãÉêÖÉåÅó=ëíçé=êçìíáåÉ= R
NKQKS oÉëí~êí=éêçÅÉÇìêÉ= S
NKR `çåÑçêã~åÅÉ=ïáíÜ=ëí~åÇ~êÇë= S
NKRKN bìêçéÉ= S
NKRKO kçêíÜ=^ãÉêáÅ~= S
NKRKP bñíÉêå~ä=ÉèìáéãÉåí= S
2. Description 7
OKN _á~ÅçêÉ=qNMM=áåëíêìãÉåí= T
OKNKN _ìÑÑÉê=íê~ó=~åÇ=äÉÑí=éìãé=Åçãé~êíãÉåí= U
OKNKO t~ëíÉ=~åÇ=ï~íÉê=íê~ó=~åÇ=êáÖÜí=éìãé=Åçãé~êíãÉåí= V
OKNKP p~ãéäÉ=Åçãé~êíãÉåí= NM
OKNKQ pÉåëçê=ÅÜáé=éçêí= NN
OKO fåÇáÅ~íçêë=~åÇ=ëïáíÅÜÉë= NO
OKOKN pí~íìë=ä~ãéë= NO
OKOKO j~áåë=éçïÉê=ëïáíÅÜ= NO
OKOKP `çãéìíÉê=ÅçååÉÅíáçåë= NP
OKP iáèìáÇ=Ü~åÇäáåÖ=ëóëíÉã= NP
OKPKN lîÉêîáÉï= NP
OKPKO ^ìíçë~ãéäÉê= NQ
OKPKP jáÅêçéä~íÉë=~åÇ=ê~Åâë= NR
OKPKQ iáèìáÇ=ëìééäó=ÄäçÅâ= NT
OKPKR _ìÑÑÉê=ëÉäÉÅíçê=î~äîÉ= NU
OKPKS _ìÑÑÉê=ÇÉÖ~ëëÉê= NU
OKPKT fåíÉÖê~íÉÇ=  JcäìáÇáÅ=`~êíêáÇÖÉ=Efc`F= NV
OKPKU cäçï=ÅÉääë= NV
OKPKV p~ãéäÉ=Ü~åÇäáåÖ= OM
OKPKNM pí~åÇÄó=ãçÇÉ=OM
OKQ aÉíÉÅíáçå=ëóëíÉã= OM
OKQKN pÉåëçê=ÅÜáé= ON
OKR qÉãéÉê~íìêÉ=Åçåíêçä= ON
OKRKN ^å~äóëáë=íÉãéÉê~íìêÉ= ON
OKRKO p~ãéäÉ=Åçãé~êíãÉåí=íÉãéÉê~íìêÉ= OO
OKS pçÑíï~êÉ= OO

Contents _________________________________________________________________________
áá================================= _á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=
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3. Operation 23
PKN pí~êíáåÖ=íÜÉ=ëóëíÉã= OP
PKO mêÉé~êáåÖ=íÜÉ=ëóëíÉã=Ñçê=~=êìå= OQ
PKOKN mêÉé~êáåÖ=ÄìÑÑÉêë= OQ
PKOKO pÉííáåÖ=ìé=íÜÉ=äáèìáÇ=ëóëíÉã= OR
PKP _~ëáÅ=çéÉê~íáåÖ=éêçÅÉÇìêÉë= OT
PKPKN fåëÉêíáåÖ=íÜÉ=ëÉåëçê=ÅÜáé= OT
PKPKO fåáíá~íáåÖ=íÜÉ=äáèìáÇ=Ü~åÇäáåÖ=ëóëíÉã= OV
PKPKP pÉííáåÖ=íÜÉ=íÉãéÉê~íìêÉ= OV
PKQ mêÉé~êáåÖ=ë~ãéäÉë=~åÇ=êÉ~ÖÉåíë= PM
PKQKN bàÉÅíáåÖ=íÜÉ=ê~Åâ=íê~ó= PM
PKQKO mêÉé~êáåÖ=ë~ãéäÉë= PN
PKQKP `Ü~åÖáåÖ=êÉ~ÖÉåí=ê~Åâë= PP
PKQKQ mêÉé~êáåÖ=êÉ~ÖÉåíë= PQ
PKQKR fåëí~ääáåÖ=íÜÉ=ê~Åâ=íê~ó= PR
PKR pí~êíáåÖ=íÜÉ=êìå= PS
PKS cáåáëÜáåÖ=íÜÉ=êìå= PT
PKSKN pí~åÇÄó=ãçÇÉ= PT
PKSKO t~ëÜ=ÄìÑÑÉê=íìÄáåÖ= PT
PKSKP pÜìíÇçïå= PT
A. Installation 39
^KN dÉåÉê~ä=ÖìáÇÉäáåÉë= PV
^KO pé~ÅÉ=êÉèìáêÉãÉåíë= PV
^KP j~áåë=éçïÉê=ëìééäó= QM
^KQ eÉ~íáåÖ=~åÇ=îÉåíáä~íáçå= QM
^KR jçîáåÖ=íÜÉ=áåëíêìãÉåí=ïáíÜáå=íÜÉ=ä~Ä= QM
^KRKN pÜìííáåÖ=Ççïå=íÜÉ=ëóëíÉã= QM
^KRKO `çååÉÅíáåÖ=íç=ã~áåë=éçïÉê= QN
^KRKP `çååÉÅíáåÖ=íÜÉ=áåëíêìãÉåí=íç=íÜÉ=m`= QN
^KS fåëí~ääáåÖ=íÜÉ=ëçÑíï~êÉ= QO
B. Maintenance 43
_KN fåíêçÇìÅíáçå= QP
_KO j~áåíÉå~åÅÉ=ëìãã~êó= QQ
_KOKN rëÉê=ã~áåíÉå~åÅÉ=çéÉê~íáçåë= QQ
_KOKO rëÉê=ëÉêîáÅÉ=çéÉê~íáçåë= QR
_KOKP mêÉîÉåíáîÉ=ã~áåíÉå~åÅÉ= QR
_KP rëÉê=ã~áåíÉå~åÅÉ=çéÉê~íáçåë= QS
_KPKN j~íÉêá~äë=êÉèìáêÉÇ= QS
_KPKO `äÉ~åáåÖ=íÜÉ=áåëíêìãÉåí= QT
_KPKP `ÜÉÅâáåÖ=Ñçê=äÉ~âë= QU
_KPKQ `äÉ~åáåÖ=~åÇ=ÇáëáåÑÉÅíáåÖ=íÜÉ=Ñäçï=ëóëíÉã= RM
_KPKR kçêã~äáòáåÖ=íÜÉ=ÇÉíÉÅíçê= RO

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_KQ rëÉê=ëÉêîáÅÉ=çéÉê~íáçåë= RP
_KQKN póëíÉã=ÅÜÉÅâ= RP
_KQKO pìéÉêÅäÉ~å= RS
_KQKP léÉåáåÖ=íÜÉ=ë~ãéäÉ=Åçãé~êíãÉåí= RS
_KQKQ `äÉ~åáåÖ=íÜÉ=ë~ãéäÉ=Åçãé~êíãÉåí= RT
_KQKR `äÉ~åáåÖ=íÜÉ=åÉÉÇäÉ=~åÇ=íÜÉ=äáèìáÇ=ëìééäó=ÄäçÅâ= RU
_KQKS oÉãçîáåÖ=íÜÉ=ë~ãéäÉ=Åçãé~êíãÉåí=Äçñ= RU
_KR pÜìííáåÖ=Ççïå=íÜÉ=ëóëíÉã= SM
_KRKN pí~åÇÄó= SM
_KRKO pÜìíÇçïå= SM
C. Troubleshooting 63
`KN póëíÉãJêÉä~íÉÇ=éêçÄäÉãë= SP
`KNKN fåëíêìãÉåí=ÜóÖáÉåÉ= SP
`KNKO fåëíêìãÉåí=ÅçåëáÇÉê~íáçåë= SP
`KNKP _ìÑÑÉê=ÅçåëáÇÉê~íáçåë= SQ
`KNKQ p~ãéäÉ=ÅçåëáÇÉê~íáçåë= SQ
`KNKR cçê=ÑìêíÜÉê=ÜÉäé= SQ
`KO ^ëë~óJêÉä~íÉÇ=éêçÄäÉãë= SQ
`KOKN iáÖ~åÇ=ÅçåëáÇÉê~íáçåë= SQ
`KOKO p~ãéäÉ=ÅçåëáÇÉê~íáçåë= SR
`KOKP cçê=~ÇÇáíáçå~ä=ÜÉäé= SR
D. Specifications 67
aKN dÉåÉê~ä= ST
aKO mêçÅÉëëáåÖ=ìåáí= ST
aKP cäçï=ÅÉääë= SU
aKQ póëíÉã=ÅçåíêçääÉê=~åÇ=ëçÑíï~êÉ= SU
aKR oÉ~ÖÉåí=ê~Åâë= SU
aKS jáÅêçéä~íÉë= SV
aKT iáèìáÇ=Åçåí~áåÉêë= SV
aKTKN _ìÑÑÉê=íê~ó= SV
aKTKO t~ëíÉ=~åÇ=ï~íÉê=íê~ó= SV
aKU `ÜÉãáÅ~ä=êÉëáëí~åÅÉ= SV
E. Technical description 71
bKN aÉíÉÅíáçå=éêáåÅáéäÉ= TN
bKNKN pìêÑ~ÅÉ=éä~ëãçå=êÉëçå~åÅÉ= TN
bKNKO tÜ~í=pmo=ãÉ~ëìêÉë= TO
bKO cäçï=ëóëíÉã= TP
bKOKN _ìÑÑÉê=ÇÉÖ~ëëÉê= TQ
bKOKO `ççäáåÖ=ìåáí= TQ
Index 75

Contents _________________________________________________________________________
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______________________________________________________________________ Introduction
_á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=================== N=
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1. Introduction
_á~ÅçêÉ=qNMM=Ñêçã=_á~ÅçêÉ=áë=~=ÜáÖÜ=éÉêÑçêã~åÅÉ=ëóëíÉã=Ñçê=êÉ~äJíáãÉ=
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íÉÅÜåçäçÖó=EpmoFK=qÜÉ=ëóëíÉã=áë=áåíÉåÇÉÇ=Ñçê=êÉëÉ~êÅÜ=~åÇ=ÇÉîÉäçéãÉåí=
~ÅíáîáíáÉë=êÉèìáêáåÖ=ÑäÉñáÄáäáíó=áå=~ëë~ó=ëÉíìéK==
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êìååáåÖ=_á~ÅçêÉ=qNMM=`çåíêçä=pçÑíï~êÉK=qÜÉ=ëçÑíï~êÉ=áë=ïáò~êÇ=Ä~ëÉÇ=
Ñçê=É~ëÉ=çÑ=ìëÉI=Äìí=~Çî~åÅÉÇ=ìëÉêë=Å~å=ÇÉîÉäçé=~åÇ=ÅìëíçãáòÉ=ãÉíÜçÇë=
íç=ëìáí=íÜÉáê=åÉÉÇëK==
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Çóå~ãáÅ=ê~åÖÉK=qÜÉ=ÇÉëáÖå=çÑ=íÜÉ=Ñäçï=ÅÉääë=áë=çéíáãáòÉÇ=Ñçê=~ÅÅìê~íÉ=
êÉÑÉêÉåÅÉ=ëìÄíê~ÅíáçåK=^å=áåíÉÖê~íÉÇ=ÄìÑÑÉê=ÇÉÖ~ëëÉê=Éäáãáå~íÉë=
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Figure 1-1. The Biacore T100 system.
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1.1 Biacore®T100 system

Introduction_______________________________________________________________________
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1.4.1 Rating information
fåëíêìãÉåí=ê~íáåÖë=~êÉ=éêáåíÉÇ=çå=íÜÉ=ã~áåë=áåéìí=é~åÉä=~í=íÜÉ=êÉ~ê=çÑ=
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1.2 The Instrument Handbook
1.3 Associated documentation
1.4 Safety information

______________________________________________________________________ Introduction
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MAINS INLET
Disconnect cord
before opening
Autorange
100-240 ~ 50-60Hz
6.3A
MAINS POWER
ATTENTION
For protection against
fire hazard replace
only with same type
and rating of fuses
2xT6.3AH 250
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Figure 1-2. Rating information on the mains input panel.
1.4.2 Safety labels
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IMPORTANT INFORMATION
Read Manual before operation
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Figure 1-3. Safety label.
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Introduction_______________________________________________________________________
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1.4.3 Safety instructions
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~åÇ=âÉÉé=óçìê=Ü~åÇë=~åÇ=äççëÉ=áíÉãë=çÑ=ÅäçíÜáåÖ=Ñêçã=íÜÉ=ê~Åâ=íê~ó=
ïÜÉå=áí=ãçîÉë=áåíç=íÜÉ=áåëíêìãÉåíK=^=íáãÉê=çå=íÜÉ=ÅçãéìíÉê=ëÅêÉÉå=
áåÇáÅ~íÉë=ïÜÉå=íÜÉ=ê~Åâ=íê~ó=ïáää=ÄÉ=~ìíçã~íáÅ~ääó=ãçîÉÇ=áåíç=íÜÉ=
áåëíêìãÉåíK=
t^okfkd>= jlsfkd=m^oqp=
^äï~óë=ëÜìí=Ççïå=íÜÉ=áåëíêìãÉåí=~åÇ=ëïáíÅÜ=çÑÑ=ã~áåë=éçïÉê=ÄÉÑçêÉ=
çéÉåáåÖ=íÜÉ=ë~ãéäÉ=Åçãé~êíãÉåíK=
t^okfkd>== eb^sv=l_gb`q= =
_á~ÅçêÉ=qNMM=ïÉáÖÜë=~Äçìí=SM=âÖK=^í=äÉ~ëí=íïç=éÉçéäÉ=~êÉ=êÉèìáêÉÇ=
íç=äáÑí=íÜÉ=áåëíêìãÉåíK=

______________________________________________________________________ Introduction
_á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=================== R=
îÉêëáçå=^^=
=
=
1.4.4 Disposal procedures
cçääçï=~ééäáÅ~ÄäÉ=å~íáçå~ä=~åÇLçê=äçÅ~ä=êÉÖìä~íáçåë=Ñçê=íÜÉ=Çáëéçë~ä=çÑ=
ÅÜÉãáÅ~äë=~åÇ=çíÜÉê=ã~íÉêá~äëK=
_á~ÅçêÉ=qNMM=Åçåí~áåë=~=äáíÜáìã=Ä~Åâìé=Ä~ííÉêóI=ïÜáÅÜ=ãìëí=åçí=ÄÉ=
ÇáëéçëÉÇ=çÑ=áå=ÑáêÉK=qÜÉ=Ä~ííÉêó=ã~ó=çåäó=ÄÉ=ÅÜ~åÖÉÇ=Äó=~=_á~ÅçêÉ=
pÉêîáÅÉ=ÉåÖáåÉÉêK=
1.4.5 Emergency stop routine
`~ìíáçå>=
aç=åçí=äÉ~îÉ=íÜÉ=ëóëíÉã=áå=~å=ÉãÉêÖÉåÅó=ëíçé=ÅçåÇáíáçåK=^äï~óë=
Ñçääçï=íÜÉ=êÉëí~êí=éêçÅÉÇìêÉ=EëÉÉ=pÉÅíáçå=NKQKSF=áÑ=éçëëáÄäÉI=íç=êÉëíçêÉ=
íÜÉ=éêçÅÉëëáåÖ=ìåáí=áåíç=åçêã~ä=ÅçåÇáíáçåK==
fÑ=íÜÉêÉ=áë=íáãÉ=
qç=ëíçé=~=êìå=ìåÇÉê=ÅçåíêçääÉÇ=ÅçåÇáíáçåë=ÄÉÑçêÉ=áí=áë=ÅçãéäÉíÉI=ÅÜççëÉ=
Run:Stop Run=Ñêçã=íÜÉ=ãÉåì=Ä~êK=qÜáë=ïáää=ëíçé=íÜÉ=êìå=~í=íÜÉ=ÉåÇ=çÑ=
íÜÉ=ÅìêêÉåí=ÅóÅäÉK==
få=~å=ÉãÉêÖÉåÅó=ëáíì~íáçå=
qç=ëíçé=~=êìå=áããÉÇá~íÉäó=áå=~å=ÉãÉêÖÉåÅó=ëáíì~íáçåI=éêÉëë=Ctrl-Break=
ECtrl-PauseF=çå=íÜÉ=âÉóÄç~êÇK=
`~ìíáçå>=
aç=åçí=ìëÉ=Ctrl-Break=ìåäÉëë=óçì=åÉÉÇ=íç=ëíçé=~ää=çéÉê~íáçåë=
áããÉÇá~íÉäó=EáÑ=íÜÉêÉ=áë=~=êáëâ=çÑ=áåàìêóI=Ç~ã~ÖÉ=çê=äçëë=çÑ=î~äì~ÄäÉ=
ã~íÉêá~äFK=
få=~å=ÉãÉêÖÉåÅó=ëáíì~íáçåW=
NK= mêÉëë=Ctrl-Break=çå=íÜÉ=âÉóÄç~êÇK=qÜáë=ïáää=áããÉÇá~íÉäó=ëíçé=~ää=
ÅìêêÉåí=áåëíêìãÉåí=çéÉê~íáçåë=áåÅäìÇáåÖ=ÄìÑÑÉê=Ñäçï=~åÇ=Ç~í~=
ÅçääÉÅíáçåK=
OK= få=íÜÉ=Çá~äçÖ=Äçñ=íÜ~í=~ééÉ~êëI=ÅäáÅâ=Yes=áÑ=óçì=ï~åí=íç=ï~ëÜ=íÜÉ=
ëóëíÉã=ïáíÜ=êìååáåÖ=ÄìÑÑÉêK=vçì=ëÜçìäÇ=Çç=íÜáë=áÑ=éçëëáÄäÉK=qÜÉ=
ï~ëÜ=çéÉê~íáçå=í~âÉë=~Äçìí=P=ãáåìíÉëK=
kçíÉW== fÑ=óçì=ëìëéÉÅí=~åó=Éêêçê=áå=íÜÉ=ëóëíÉãI=Éñáí=Ñêçã=_á~ÅçêÉ=qNMM=
`çåíêçä=pçÑíï~êÉK=qìêå=çÑÑ=éçïÉê=íç=íÜÉ=áåëíêìãÉåí=ïáíÜ=íÜÉ=
MAINS POWER=ëïáíÅÜ=~í=íÜÉ=Ä~Åâ=çÑ=íÜÉ=ìåáíK= =

Introduction_______________________________________________________________________
S== = _á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=
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îÉêëáçå=^^=
=
=
1.4.6 Restart procedure
qç=êÉëí~êí=_á~ÅçêÉ=qNMMW=
NK= qìêå=çå=ã~áåë=éçïÉê=~åÇ=ÅÜÉÅâ=íÜ~í=íÜÉ=áåëíêìãÉåí=ëí~êíë=
åçêã~ääó=EëÉÉ=pÉÅíáçå=PKNFK=
OK= fÑ=íÜÉ=éêçÄäÉã=éÉêëáëíëI=êìå=íÜÉ=ëÉêîáÅÉ=íççä=System Check EëÉÉ=
pÉÅíáçå=_KQKNFK=fÑ=~åó=Éêêçê=áë=ÇÉíÉÅíÉÇ=Å~ää=óçìê=_á~ÅçêÉ=pÉêîáÅÉ=
êÉéêÉëÉåí~íáîÉK=
fÑ=óçì=åÉÉÇ=íç=ÅäÉ~å=íÜÉ=äáèìáÇ=Ü~åÇäáåÖ=ëóëíÉãI=ÉàÉÅí=íÜÉ=ëÉåëçê=ÅÜáé=
~åÇ=áåëÉêí=~=ã~áåíÉå~åÅÉ=ÅÜáéK=pÉÉ=^ééÉåÇáñ=_=Ñçê=ÑìêíÜÉê=áåëíêìÅíáçåëK=
=
1.5.1 Europe
_á~ÅçêÉ=qNMM=mêçÅÉëëáåÖ=råáí=ãÉÉíë=íÜÉ=êÉèìáêÉãÉåíë=çÑ=íÜÉ=ÑçääçïáåÖ=
ÇáêÉÅíáîÉëI=íÜêçìÖÜ=íÜÉ=êÉÑÉêÉåÅÉÇ=Ü~êãçåáòÉÇ=ëí~åÇ~êÇëW=
European directive Harmonized standard
TPLOPLbb`I=içï=îçäí~ÖÉ=ÇÉîáÅÉë= bk=SN=MNMJN=EOMMNFI==
bk=SN=MNMJNJMUN=EOMMOFI=p~ÑÉíó=
êÉèìáêÉãÉåíë=Ñçê=ÉäÉÅíêáÅ~ä=
ÉèìáéãÉåí=Ñçê=ãÉ~ëìêÉãÉåíI=
Åçåíêçä=~åÇ=ä~Äçê~íçêó=ìëÉK=
UVLPPSLbb`I=bäÉÅíêçã~ÖåÉíáÅ=
Åçãé~íáÄáäáíó=
bk=SN=POSJN=ENVVTF=~åÇ=
~ãÉåÇãÉåí=^N=ENVVUF=~åÇ=^O=
EOMMNFI=bj`=êÉèìáêÉãÉåíë=Ñçê=
`ä~ëë=_=ÉèìáéãÉåíK=
1.5.2 North America
_á~ÅçêÉ=qNMM=mêçÅÉëëáåÖ=råáí=ãÉÉíë=íÜÉ=ÑçääçïáåÖ=ë~ÑÉíó=ëí~åÇ~êÇëW=
 ri=SNMNM^JN=~åÇ=`^kL`p^J`KOOKO=kç=NMNMKNI=p~ÑÉíó=
êÉèìáêÉãÉåíë=Ñçê=ÉäÉÅíêáÅ~ä=ÉèìáéãÉåí=Ñçê=ãÉ~ëìêÉãÉåíI=Åçåíêçä=~åÇ=
ä~Äçê~íçêó=ìëÉK=
1.5.3 External equipment
^åó=ÉñíÉêå~ä=ÉèìáéãÉåí=EÉKÖK=ÅçãéìíÉêF=ÅçååÉÅíÉÇ=íç=íÜÉ=_á~ÅçêÉ=qNMM=
mêçÅÉëëáåÖ=råáí=ãìëí=Åçãéäó=ïáíÜ=íÜÉ=~ééäáÅ~ÄäÉ=fb`Lbk=ëí~åÇ~êÇëK
1.5 Conformance with standards

________________________________________________________________________Description
_á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=================== T=
îÉêëáçå=^^=
=
=
2. Description
_á~ÅçêÉ=qNMM=áåëíêìãÉåí=áë=~=éêçÅÉëëáåÖ=ìåáí=ïáíÜ=äáèìáÇ=Ü~åÇäáåÖI=
ë~ãéäÉ=Ü~åÇäáåÖ=~åÇ=ÇÉíÉÅíáçå=ëóëíÉãI=ÅçåíêçääÉÇ=Ñêçã=~=m`=êìååáåÖ=
_á~ÅçêÉ=qNMM=`çåíêçä=pçÑíï~êÉK==
_á~ÅçêÉ=qNMM=áåëíêìãÉåí=áë=ìëÉÇ=ïáíÜ=ëÉåëçê=ÅÜáéë=Ñêçã=_á~ÅçêÉK==
^=ê~åÖÉ=çÑ=ÅÜÉãáÅ~äë=~åÇ=êÉ~ÖÉåíë=ÇÉëáÖåÉÇ=Ñçê=ìëÉ=ïáíÜ=_á~ÅçêÉ=qNMM=
áë=~äëç=~î~áä~ÄäÉK=
Sample
compartment door
Left pump
compartment door
Sample
compartment
inspection window
Rack tray port
Buffer tray
=
Status lamps
Sensor chip
port
Right pump
compartment
door
Waste and
water tray
Figure 2-1. Biacore T100 instrument.
qÜÉ=êÉãçî~ÄäÉ=ëÉåëçê=ÅÜáé=áë=áåëÉêíÉÇ=áåíç=íÜÉ=ëÉåëçê=ÅÜáé=éçêí=~åÇ=
ÇçÅâë=áåíç=íÜÉ=áåëíêìãÉåíK=léíáÅ~ä=ÇÉíÉÅíáçå=çÅÅìêë=áå=Ñäçï=ÅÉääë=íÜ~í=
~êÉ=ÑçêãÉÇ=Äó=íÜÉ=ëÉåëçê=ÅÜáé=~åÇ=íÜÉ=fåíÉÖê~íÉÇ=JcäìáÇáÅ=`~êíêáÇÖÉ=
Efc`F=~ë=é~êí=çÑ=íÜÉ=ÇçÅâáåÖ=éêçÅÉëëK=
_çííäÉë=Åçåí~áåáåÖ=ÄìÑÑÉê=~êÉ=éä~ÅÉÇ=çå=íÜÉ=ÄìÑÑÉê=íê~óK=ré=íç=Ñçìê=
ÇáÑÑÉêÉåí=ÄìÑÑÉêë=Å~å=ÄÉ=ìëÉÇK=^=ÄìÑÑÉê=ëÉäÉÅíçê=î~äîÉ=ÇÉíÉêãáåÉë=ïÜáÅÜ=
ÄìÑÑÉê=Ñäçïë=çîÉê=íÜÉ=ëÉåëçê=ëìêÑ~ÅÉK=
2.1 Biacore T100 instrument

Description ________________________________________________________________________
U== = _á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=
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îÉêëáçå=^^=
=
=
^=ï~ëíÉ=ÄçííäÉ=~åÇ=~=ÄçííäÉ=Åçåí~áåáåÖ=ï~íÉê=~êÉ=éä~ÅÉÇ=çå=íÜÉ=ï~ëíÉ=
~åÇ=ï~íÉê=íê~óK=
oÉ~ÖÉåíë=~åÇ=ë~ãéäÉë=~êÉ=äç~ÇÉÇ=áåíç=~=êÉ~ÖÉåí=ê~Åâ=~åÇ=~=ãáÅêçéä~íÉI=
çê=áåíç=~=ÅçãÄáåÉÇ=ë~ãéäÉ=~åÇ=êÉ~ÖÉåí=ê~ÅâK=qÜÉ=êÉ~ÖÉåí=ê~Åâ=~åÇLçê=
ãáÅêçéä~íÉ=~êÉ=éä~ÅÉÇ=çå=~=ê~Åâ=íê~óK=qÜÉ=ê~Åâ=íê~ó=çê=íÜÉ=ë~ãéäÉ=~åÇ=
êÉ~ÖÉåí=ê~Åâ=áë=áåëÉêíÉÇ=áåíç=íÜÉ=ê~Åâ=íê~ó=éçêíK==
aìêáåÖ=~=êìåI=ë~ãéäÉë=~åÇ=êÉ~ÖÉåíë=~êÉ=~ìíçã~íáÅ~ääó=áåàÉÅíÉÇ=áåíç=íÜÉ=
ÇÉíÉÅíáçå=ìåáíK=
2.1.1 Buffer tray and left pump compartment
qÜÉ=ÄìÑÑÉê=íê~ó=çå=íÜÉ=äÉÑí=çÑ=íÜÉ=áåëíêìãÉåí=ÜçäÇë=ìé=íç=Ñçìê=ÄçííäÉë=
Ñçê=êìååáåÖ=ÄìÑÑÉêK==
qÜÉ=ÄìÑÑÉê=íê~ó=áë=ÇÉëáÖåÉÇ=íç=ÜçäÇ=ëí~åÇ~êÇ=ÄçííäÉë=íÜêÉ~ÇÉÇ=Ñçê=ëÅêÉï=
Å~éëK=cçìê=ÄçííäÉë=~åÇ=Å~éë=ïáíÜ=Ö~ëâÉíë=~êÉ=éêçîáÇÉÇ=ïáíÜ=íÜÉ=ëóëíÉãW=
çåÉ=NJäáíÉê=ÄçííäÉ=~åÇ=íÜêÉÉ=ORM=ãä=ÄçííäÉëK=
qÜÉ=äÉÑí=éìãé=Åçãé~êíãÉåí=ÜçìëÉë=~=ÄìÑÑÉê=ëÉäÉÅíçê=î~äîÉI=íïç=ëóêáåÖÉ=
éìãéë=Ñçê=ë~ãéäÉ=Ü~åÇäáåÖ=~åÇ=Ñäçï=ëóëíÉã=çéÉê~íáçåI=~åÇ=~=ÄìÑÑÉê=
ÇÉÖ~ëëÉêK=qç=çéÉå=íÜÉ=éìãé=Åçãé~êíãÉåíI=éêÉëë=çå=íÜÉ=áååÉê=ÉÇÖÉ=çÑ=
íÜÉ=ÇççêK=
Holder for unused
buffer tubes
Buffer degasser
Syringe pumps
Buffer selector valve
Buffer tray
=
Figure 2-2. Left pump compartment, opened.

________________________________________________________________________Description
_á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=================== V=
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=
=
_ìÑÑÉê=íìÄáåÖ=~åÇ=ëÉäÉÅíçê=î~äîÉ=
qÜÉ=ÄìÑÑÉê=íìÄÉëI=ã~êâÉÇ=^I=_I=`I=~åÇ=aI=~êÉ=ÅçååÉÅíÉÇ=íç=íÜÉ=áåéìíë=
çÑ=~=ÄìÑÑÉê=ëÉäÉÅíçê=î~äîÉK=qÜÉ=ëÉäÉÅíçê=î~äîÉ=ÇÉíÉêãáåÉë=ïÜáÅÜ=çÑ=íÜÉ=
ÄìÑÑÉêë=áë=ìëÉÇ=ÇìêáåÖ=~=êìåK=_ìÑÑÉê=ëÉäÉÅíáçå=áë=ÅçåíêçääÉÇ=Ñêçã=íÜÉ=
ëçÑíï~êÉÔÄìÑÑÉê=^=áë=ëÉäÉÅíÉÇ=Äó=ÇÉÑ~ìäíK=
^íí~ÅÜ=ìåìëÉÇ=ÄìÑÑÉê=íìÄÉë=íç=íÜÉ=ÜçäÇÉê=áåëáÇÉ=íÜÉ=éìãé=Åçãé~êíãÉåí=
ÇççêK=
_ìÑÑÉê=ÇÉÖ~ëëÉê=
qÜÉ=Ö~ë=ÅçåíÉåí=çÑ=íÜÉ=êìååáåÖ=ÄìÑÑÉê=áë=êÉÇìÅÉÇ=íç=~=äçï=äÉîÉä=Äó=~=
î~Åììã=ÇÉÖ~ëëÉêK=qÜáë=Éäáãáå~íÉë=íÜÉ=åÉÉÇ=íç=ÇÉÖ~ë=êìååáåÖ=ÄìÑÑÉê=
ÄÉÑçêÉ=ìëÉK=
kçíÉW= qÜÉ=ÄìÑÑÉê=íìÄáåÖ=ëÜçìäÇ=~äï~óë=ÄÉ=ÅçååÉÅíÉÇ=îá~=íÜÉ=ÄìÑÑÉê=
ÇÉÖ~ëëÉêK=aç=åçí=ÇáëÅçååÉÅí=íìÄÉë=Ñêçã=íÜÉ=ÇÉÖ~ëëÉê=ÉîÉå=áÑ=
óçì=ìëÉ=ÇÉÖ~ëëÉÇ=ÄìÑÑÉêK=
2.1.2 Waste and water tray and right pump compartment
qÜÉ=ï~ëíÉ=~åÇ=ï~íÉê=íê~ó=çå=íÜÉ=êáÖÜí=çÑ=íÜÉ=áåëíêìãÉåí=ÜçäÇë=çåÉ=
OJäáíÉê=ÄçííäÉ=Ñçê=ï~ëíÉ=ëçäìíáçåëI=~åÇ=çåÉ=RMM=ãä=ÄçííäÉ=Ñçê=ÇáëíáääÉÇ=
ï~íÉêK=
låÉ=OJäáíÉê=ÄçííäÉ=~åÇ=Å~é=ïáíÜ=íìÄÉ=ÑáííáåÖëI=~åÇ=çåÉ=RMM=ãä=ÄçííäÉ=~åÇ=
Å~é=ïáíÜ=Ö~ëâÉí=~êÉ=éêçîáÇÉÇ=ïáíÜ=íÜÉ=ëóëíÉãK=
qÜÉ=êáÖÜí=éìãé=Åçãé~êíãÉåí=ÜçìëÉë=~=éÉêáëí~äíáÅ=éìãé=Ñçê=ëìééäó=çÑ=
ÄìÑÑÉê=~åÇ=ï~íÉê=íç=íÜÉ=äáèìáÇ=ëìééäó=ÄäçÅâK=qç=çéÉå=íÜÉ=éìãé=
Åçãé~êíãÉåíI=éêÉëë=çå=íÜÉ=áååÉê=ÉÇÖÉ=çÑ=íÜÉ=ÇççêK=
=
Peristaltic pump
Waste and water tray
=
Figure 2-3. Waste and water tray and right pump compartment.

Description ________________________________________________________________________
NM== = _á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=
==
îÉêëáçå=^^=
=
=
qÜÉ=ï~ëíÉ=íìÄÉë=~êÉ=~íí~ÅÜÉÇ=ïáíÜ=íìÄÉ=ÑáííáåÖë=íç=íÜÉ=ï~ëíÉ=Å~éK=_ÉÑçêÉ=
ëí~êíáåÖ=~=êìåI=ã~âÉ=ëìêÉ=íÜ~í=íÜÉ=íìÄÉ=ÑáííáåÖë=~êÉ=íáÖÜíÉåÉÇ=~åÇ=íÜ~í=
íÜÉ=ï~ëíÉ=ÄçííäÉ=áë=ÉãéíóK=
kçíÉW= qÜÉ=ï~ëíÉ=ÄçííäÉ=~åÇ=íÜÉ=Å~é=ãìëí=ÄÉ=çÑ=íÜÉ=ë~ãÉ=íóéÉ=~åÇ=ëáòÉ=
~ë=íÜÉ=çåÉë=ÇÉäáîÉêÉÇ=ïáíÜ=íÜÉ=ëóëíÉã=íç=~îçáÇ=éêÉëëìêÉ=
ÇáëíìêÄ~åÅÉë=áå=íÜÉ=äáèìáÇ=Ü~åÇäáåÖ=ëóëíÉãK=fÑ=íÜÉ=çìíäÉí=áë=
äçïÉê=íÜ~å=íÜÉ=íçé=çÑ=íÜÉ=ÄçííäÉI=íÜÉêÉ=ïáää=ÄÉ=~=éêÉëëìêÉ=
áãÄ~ä~åÅÉ=áå=íÜÉ=Ñäçï=ëóëíÉã=~åÇ=äáèìáÇ=ã~ó=ÄÉ=ëáéÜçåÉÇ=çìí=
çÑ=íÜÉ=fc`K=
2.1.3 Sample compartment
qÜÉ=ë~ãéäÉ=Åçãé~êíãÉåí=ÜçäÇë=íÜÉ=~ìíçë~ãéäÉê=~åÇ=íÜÉ=ë~ãéäÉ=
áåàÉÅíáçå=ìåáí=EëÉÉ=pÉÅíáçå=OKPKOFK=qÜÉ=ê~Åâ=íê~ó=éçêí=çå=íÜÉ=Ñêçåí=çÑ=íÜÉ=
áåëíêìãÉåí=áë=ÅçåíêçääÉÇ=Ñêçã=íÜÉ=ëçÑíï~êÉK==
iç~ÇáåÖ=ë~ãéäÉë=~åÇ=êÉ~ÖÉåíë=
qÜÉ=ìëÉê=äç~Çë=íÜÉ=ê~Åâ=íê~óI=Å~êêóáåÖ=íÜÉ=ë~ãéäÉ=~åÇ=êÉ~ÖÉåí=ê~ÅâëI=
áåíç=íÜÉ=ê~Åâ=íê~ó=éçêí=ÄÉÑçêÉ=ëí~êíáåÖ=íÜÉ=êìåK=qÜÉêÉ=áë=åç=~ÅÅÉëë=íç=íÜÉ=
ë~ãéäÉ=Åçãé~êíãÉåí=ÇìêáåÖ=~=ïáò~êÇ=êìå=çê=ãÉíÜçÇ=êìåK=vçì=ã~ó=
~ÇÇ=ë~ãéäÉë=ÇìêáåÖ=~=ã~åì~ä=êìåK=
=
=
=
Figure 2-4. Loading the rack tray into the rack tray port.

________________________________________________________________________Description
_á~ÅçêÉ=qNMM=fåëíêìãÉåí=e~åÇÄççâ=================== NN=
îÉêëáçå=^^=
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2.1.4 Sensor chip port
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Figure 2-5. Inserting a sensor chip.
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Description ________________________________________________________________________
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2.2.1 Status lamps
Ready=EÖêÉÉåF=
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System=EêÉÇF=
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ä~ãé=äáÖÜíë=áå=çíÜÉê=ÅáêÅìãëí~åÅÉëI=íìêå=çÑÑ=íÜÉ=áåëíêìãÉåí=~åÇ=Å~ää=
óçìê=_á~ÅçêÉ=ëÉêîáÅÉ=êÉéêÉëÉåí~íáîÉK=
Temperature=EóÉääçïF=
iáÖÜíë=ëíÉ~Çáäó=ïÜÉå=íÜÉ=íÉãéÉê~íìêÉ=~í=íÜÉ=Ñäçï=ÅÉää=áë=ëí~ÄäÉ=~í=íÜÉ=
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cä~ëÜÉë=ïÜÉå=íÜÉ=íÉãéÉê~íìêÉ=áë=åçí=ëí~ÄäÉK=
Sensor chip=EÖêÉÉåF=
iáÖÜíë=ïÜÉå=~=ëÉåëçê=ÅÜáé=áë=ÇçÅâÉÇ=~åÇ=êÉ~ÇóK=
Run=EÖêÉÉåF=
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2.2.2 Mains power switch
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MAINS INLET
Disconnect cord
before opening
Autorange
100-240 ~ 50-60Hz
6.3A
MAINS POWER
ATTENTION
For protection against
fire hazard replace
only with same type
and rating of fuses
2xT6.3AH 250
Mains
inlet
Mains
switch
Mains
fuses
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Figure 2-6. Mains input panel at the rear of the instrument.
2.2 Indicators and switches
ready
run
sensor chip
temperature
system
=